Journal: International Journal of Oral Science
Article Title: Upregulation of epidermal growth factor receptor 4 in oral leukoplakia
Figure Lengend Snippet: Protein expression profiles of EGFR family in NOM, OLP and LP. Routine staining for HE and immunohistochemical analysis for the expression of ErbB1, ErbB2, ErbB3 and ErbB4 in NOM, OLP and LP tissues using commercially available monoclonal antibodies and the Envision technique using DAB as an indicator agent. Expression of ErbB1 and ErbB4 was detected in all tissue specimens, while those of ErbB2 and ErbB3 were slightly expressed in NOM, and LP. Magnifications: ×40 ( A , B and C ) and ×100 ( a - o ).* indicates basal cell layer; ⋆ indicates prickle cell layer; ★ indicates keratinized layer. DAB, 3,3′-diaminobenzidine tetrahydrochloride; EGFR, epidermal growth factor receptor; HE, hematoxylin and eosin; LP, leukoplakia; NOM, normal oral mucosa; OLP, oral lichen planus.
Article Snippet: Using commercially available monoclonal or polyclonal antibodies and following the ENVISION technique (K4003; Dako, Copenhagen, Denmark) with 3,3′-diaminobenzidine tetrahydrochloride as the indicator agent, immunohistochemical analyses were performed for the expressions of the following EGFR family: ErbB1 (k1492; Dako, Copenhagen, Denmark), ErbB2 (427041; Nichirei, Tokyo, Japan), ErbB3 (ab15136; Abcam PLC, Cambridge, UK) and ErbB4 (sc-283; Santa Cruz Biotechnology, Santa Cruz, CA, USA).
Techniques: Expressing, Staining, Immunohistochemistry