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  • 93
    Millipore hela dh cells
    <t>Cx26</t> immunostaining performed in <t>Hela</t> DH transfectants overexpressing Cx26WT (top), and Cx26C169Y (bottom) proteins. Through focus confocal image sequence (z-stack) taken at 0.5 µm intervals of Hela DH cells expressing Cx26WT ( A – D ) and Cx26C169Y ( F – I ) proteins and their respective maximal projection rendering ( E and J ). Yellow arrow points to representative gap junction plaque, whereas red arrows indicate immunoreaction signals at the cell plasma membrane level, which are most likely due to unpaired connexons. Scale bar, 10 µm.
    Hela Dh Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Tocris dh 97
    Effects of melatonin receptor antagonists on the action of 5-MCA-NAT. (A) Prazosin alone caused a transient increase in intraocular pressure. (B) Graded doses of prazosin, <t>DH-97</t> and 4-P-PDOT were applied as described in Methods. The maximal reduction in intraocular pressure due to 5-MCA-NAT was 35±2% ( n =8). Values represent the mean±s.e.mean of eight independent experiments.
    Dh 97, supplied by Tocris, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Bruker Corporation g4 phoenix dh
    Effects of melatonin receptor antagonists on the action of 5-MCA-NAT. (A) Prazosin alone caused a transient increase in intraocular pressure. (B) Graded doses of prazosin, <t>DH-97</t> and 4-P-PDOT were applied as described in Methods. The maximal reduction in intraocular pressure due to 5-MCA-NAT was 35±2% ( n =8). Values represent the mean±s.e.mean of eight independent experiments.
    G4 Phoenix Dh, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    TaKaRa dh 5α
    Effects of melatonin receptor antagonists on the action of 5-MCA-NAT. (A) Prazosin alone caused a transient increase in intraocular pressure. (B) Graded doses of prazosin, <t>DH-97</t> and 4-P-PDOT were applied as described in Methods. The maximal reduction in intraocular pressure due to 5-MCA-NAT was 35±2% ( n =8). Values represent the mean±s.e.mean of eight independent experiments.
    Dh 5α, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Millar Inc marchadier dh
    Effects of melatonin receptor antagonists on the action of 5-MCA-NAT. (A) Prazosin alone caused a transient increase in intraocular pressure. (B) Graded doses of prazosin, <t>DH-97</t> and 4-P-PDOT were applied as described in Methods. The maximal reduction in intraocular pressure due to 5-MCA-NAT was 35±2% ( n =8). Values represent the mean±s.e.mean of eight independent experiments.
    Marchadier Dh, supplied by Millar Inc, used in various techniques. Bioz Stars score: 88/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Drucker Diagnostics wasserman dh
    Effects of melatonin receptor antagonists on the action of 5-MCA-NAT. (A) Prazosin alone caused a transient increase in intraocular pressure. (B) Graded doses of prazosin, <t>DH-97</t> and 4-P-PDOT were applied as described in Methods. The maximal reduction in intraocular pressure due to 5-MCA-NAT was 35±2% ( n =8). Values represent the mean±s.e.mean of eight independent experiments.
    Wasserman Dh, supplied by Drucker Diagnostics, used in various techniques. Bioz Stars score: 89/100, based on 86 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    SEARLE Company williams dh
    Effects of melatonin receptor antagonists on the action of 5-MCA-NAT. (A) Prazosin alone caused a transient increase in intraocular pressure. (B) Graded doses of prazosin, <t>DH-97</t> and 4-P-PDOT were applied as described in Methods. The maximal reduction in intraocular pressure due to 5-MCA-NAT was 35±2% ( n =8). Values represent the mean±s.e.mean of eight independent experiments.
    Williams Dh, supplied by SEARLE Company, used in various techniques. Bioz Stars score: 88/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Matreya LLC c18 dhs
    Effects of melatonin receptor antagonists on the action of 5-MCA-NAT. (A) Prazosin alone caused a transient increase in intraocular pressure. (B) Graded doses of prazosin, <t>DH-97</t> and 4-P-PDOT were applied as described in Methods. The maximal reduction in intraocular pressure due to 5-MCA-NAT was 35±2% ( n =8). Values represent the mean±s.e.mean of eight independent experiments.
    C18 Dhs, supplied by Matreya LLC, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Toyobo dh 5α
    Effects of melatonin receptor antagonists on the action of 5-MCA-NAT. (A) Prazosin alone caused a transient increase in intraocular pressure. (B) Graded doses of prazosin, <t>DH-97</t> and 4-P-PDOT were applied as described in Methods. The maximal reduction in intraocular pressure due to 5-MCA-NAT was 35±2% ( n =8). Values represent the mean±s.e.mean of eight independent experiments.
    Dh 5α, supplied by Toyobo, used in various techniques. Bioz Stars score: 88/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Biolink Corporation dh biolink
    Effects of melatonin receptor antagonists on the action of 5-MCA-NAT. (A) Prazosin alone caused a transient increase in intraocular pressure. (B) Graded doses of prazosin, <t>DH-97</t> and 4-P-PDOT were applied as described in Methods. The maximal reduction in intraocular pressure due to 5-MCA-NAT was 35±2% ( n =8). Values represent the mean±s.e.mean of eight independent experiments.
    Dh Biolink, supplied by Biolink Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Preiser Scientific williamson dh
    Effects of melatonin receptor antagonists on the action of 5-MCA-NAT. (A) Prazosin alone caused a transient increase in intraocular pressure. (B) Graded doses of prazosin, <t>DH-97</t> and 4-P-PDOT were applied as described in Methods. The maximal reduction in intraocular pressure due to 5-MCA-NAT was 35±2% ( n =8). Values represent the mean±s.e.mean of eight independent experiments.
    Williamson Dh, supplied by Preiser Scientific, used in various techniques. Bioz Stars score: 88/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Ocean Optics dh 2000
    Effects of melatonin receptor antagonists on the action of 5-MCA-NAT. (A) Prazosin alone caused a transient increase in intraocular pressure. (B) Graded doses of prazosin, <t>DH-97</t> and 4-P-PDOT were applied as described in Methods. The maximal reduction in intraocular pressure due to 5-MCA-NAT was 35±2% ( n =8). Values represent the mean±s.e.mean of eight independent experiments.
    Dh 2000, supplied by Ocean Optics, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    SI Manufacturing smith dh
    Effects of melatonin receptor antagonists on the action of 5-MCA-NAT. (A) Prazosin alone caused a transient increase in intraocular pressure. (B) Graded doses of prazosin, <t>DH-97</t> and 4-P-PDOT were applied as described in Methods. The maximal reduction in intraocular pressure due to 5-MCA-NAT was 35±2% ( n =8). Values represent the mean±s.e.mean of eight independent experiments.
    Smith Dh, supplied by SI Manufacturing, used in various techniques. Bioz Stars score: 91/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Sanofi dh artem
    Effects of melatonin receptor antagonists on the action of 5-MCA-NAT. (A) Prazosin alone caused a transient increase in intraocular pressure. (B) Graded doses of prazosin, <t>DH-97</t> and 4-P-PDOT were applied as described in Methods. The maximal reduction in intraocular pressure due to 5-MCA-NAT was 35±2% ( n =8). Values represent the mean±s.e.mean of eight independent experiments.
    Dh Artem, supplied by Sanofi, used in various techniques. Bioz Stars score: 88/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    tiangen biotech co dh 5α
    Effects of melatonin receptor antagonists on the action of 5-MCA-NAT. (A) Prazosin alone caused a transient increase in intraocular pressure. (B) Graded doses of prazosin, <t>DH-97</t> and 4-P-PDOT were applied as described in Methods. The maximal reduction in intraocular pressure due to 5-MCA-NAT was 35±2% ( n =8). Values represent the mean±s.e.mean of eight independent experiments.
    Dh 5α, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 92/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Roche liberase dh
    Viability assay performed on isolated follicles using the fluorescent probes calcein AM and ethidium homodimer-1. a and d . Isolated follicles after using collagenase NB6; b and e . Isolated follicles after using <t>Liberase</t> DH; c and f . Isolated follicles after using collagenase IA; green: live follicles; red: dead follicles; scale bar = 100 μm
    Liberase Dh, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 575 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Trinity Biotech o donnell dh
    Viability assay performed on isolated follicles using the fluorescent probes calcein AM and ethidium homodimer-1. a and d . Isolated follicles after using collagenase NB6; b and e . Isolated follicles after using <t>Liberase</t> DH; c and f . Isolated follicles after using collagenase IA; green: live follicles; red: dead follicles; scale bar = 100 μm
    O Donnell Dh, supplied by Trinity Biotech, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Millipore lignoceroyl dhs
    Viability assay performed on isolated follicles using the fluorescent probes calcein AM and ethidium homodimer-1. a and d . Isolated follicles after using collagenase NB6; b and e . Isolated follicles after using <t>Liberase</t> DH; c and f . Isolated follicles after using collagenase IA; green: live follicles; red: dead follicles; scale bar = 100 μm
    Lignoceroyl Dhs, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    ICF Macro measure dhs
    Viability assay performed on isolated follicles using the fluorescent probes calcein AM and ethidium homodimer-1. a and d . Isolated follicles after using collagenase NB6; b and e . Isolated follicles after using <t>Liberase</t> DH; c and f . Isolated follicles after using collagenase IA; green: live follicles; red: dead follicles; scale bar = 100 μm
    Measure Dhs, supplied by ICF Macro, used in various techniques. Bioz Stars score: 90/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher dh 5α e
    Viability assay performed on isolated follicles using the fluorescent probes calcein AM and ethidium homodimer-1. a and d . Isolated follicles after using collagenase NB6; b and e . Isolated follicles after using <t>Liberase</t> DH; c and f . Isolated follicles after using collagenase IA; green: live follicles; red: dead follicles; scale bar = 100 μm
    Dh 5α E, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Lyncee Tec dh t 1001
    Viability assay performed on isolated follicles using the fluorescent probes calcein AM and ethidium homodimer-1. a and d . Isolated follicles after using collagenase NB6; b and e . Isolated follicles after using <t>Liberase</t> DH; c and f . Isolated follicles after using collagenase IA; green: live follicles; red: dead follicles; scale bar = 100 μm
    Dh T 1001, supplied by Lyncee Tec, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Millipore dh β e
    Viability assay performed on isolated follicles using the fluorescent probes calcein AM and ethidium homodimer-1. a and d . Isolated follicles after using collagenase NB6; b and e . Isolated follicles after using <t>Liberase</t> DH; c and f . Isolated follicles after using collagenase IA; green: live follicles; red: dead follicles; scale bar = 100 μm
    Dh β E, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Anton Paar domed dhs 900
    Viability assay performed on isolated follicles using the fluorescent probes calcein AM and ethidium homodimer-1. a and d . Isolated follicles after using collagenase NB6; b and e . Isolated follicles after using <t>Liberase</t> DH; c and f . Isolated follicles after using collagenase IA; green: live follicles; red: dead follicles; scale bar = 100 μm
    Domed Dhs 900, supplied by Anton Paar, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Cx26 immunostaining performed in Hela DH transfectants overexpressing Cx26WT (top), and Cx26C169Y (bottom) proteins. Through focus confocal image sequence (z-stack) taken at 0.5 µm intervals of Hela DH cells expressing Cx26WT ( A – D ) and Cx26C169Y ( F – I ) proteins and their respective maximal projection rendering ( E and J ). Yellow arrow points to representative gap junction plaque, whereas red arrows indicate immunoreaction signals at the cell plasma membrane level, which are most likely due to unpaired connexons. Scale bar, 10 µm.

    Journal: Human Molecular Genetics

    Article Title: The p.Cys169Tyr variant of connexin 26 is not a polymorphism

    doi: 10.1093/hmg/ddv026

    Figure Lengend Snippet: Cx26 immunostaining performed in Hela DH transfectants overexpressing Cx26WT (top), and Cx26C169Y (bottom) proteins. Through focus confocal image sequence (z-stack) taken at 0.5 µm intervals of Hela DH cells expressing Cx26WT ( A – D ) and Cx26C169Y ( F – I ) proteins and their respective maximal projection rendering ( E and J ). Yellow arrow points to representative gap junction plaque, whereas red arrows indicate immunoreaction signals at the cell plasma membrane level, which are most likely due to unpaired connexons. Scale bar, 10 µm.

    Article Snippet: Cx26 immunoreactivity in Hela cells Hela DH cells (Sigma, cat. 96112022) were grown in DMEM (Gibco, catalog no. 41965-039) supplemented with 10% FBS (Gibco, catalog no. 10106169), plated on glass coverslips.

    Techniques: Immunostaining, Sequencing, Expressing

    Effects of melatonin receptor antagonists on the action of 5-MCA-NAT. (A) Prazosin alone caused a transient increase in intraocular pressure. (B) Graded doses of prazosin, DH-97 and 4-P-PDOT were applied as described in Methods. The maximal reduction in intraocular pressure due to 5-MCA-NAT was 35±2% ( n =8). Values represent the mean±s.e.mean of eight independent experiments.

    Journal: British Journal of Pharmacology

    Article Title: Ocular hypotensive effects of melatonin receptor agonists in the rabbit: further evidence for an MT3 receptor

    doi: 10.1038/sj.bjp.0705118

    Figure Lengend Snippet: Effects of melatonin receptor antagonists on the action of 5-MCA-NAT. (A) Prazosin alone caused a transient increase in intraocular pressure. (B) Graded doses of prazosin, DH-97 and 4-P-PDOT were applied as described in Methods. The maximal reduction in intraocular pressure due to 5-MCA-NAT was 35±2% ( n =8). Values represent the mean±s.e.mean of eight independent experiments.

    Article Snippet: 2-Phe-melatonin, 6-Cl-melatonin, 2-I-melatonin, 5-MCA-NAT, N-acetyltryptamine, prazosin, DH-97 and 4-P-PDOT, yohimbine and (±)-1- [2,3 - (dihydro - 7 - methyl - 1 H -inden-4-yl)oxy]-3 - [(1-methylethyl) amino]-2-butanol hydrochloride (ICI 118,551) were from Tocris (Bristol, U.K.).

    Techniques:

    Viability assay performed on isolated follicles using the fluorescent probes calcein AM and ethidium homodimer-1. a and d . Isolated follicles after using collagenase NB6; b and e . Isolated follicles after using Liberase DH; c and f . Isolated follicles after using collagenase IA; green: live follicles; red: dead follicles; scale bar = 100 μm

    Journal: Journal of Ovarian Research

    Article Title: A protocol to isolate and qualify purified human preantral follicles in cases of acute leukemia, for future clinical applications

    doi: 10.1186/s13048-017-0376-6

    Figure Lengend Snippet: Viability assay performed on isolated follicles using the fluorescent probes calcein AM and ethidium homodimer-1. a and d . Isolated follicles after using collagenase NB6; b and e . Isolated follicles after using Liberase DH; c and f . Isolated follicles after using collagenase IA; green: live follicles; red: dead follicles; scale bar = 100 μm

    Article Snippet: They underwent mechanical and enzymatic dissociation using either collagenase IA routinely used in our laboratory (200 IU/ml final, Sigma Aldrich), a GMP grade purified collagenase NB6 (200 IU/ml final, Serva Electrophoresis GMbH; Coger, France), or Liberase DH (0.28 Wünsch U/ml, Roche, France).

    Techniques: Viability Assay, Isolation, IA