Journal: Journal of Ovarian Research
Article Title: A protocol to isolate and qualify purified human preantral follicles in cases of acute leukemia, for future clinical applications
Figure Lengend Snippet: Viability assay performed on isolated follicles using the fluorescent probes calcein AM and ethidium homodimer-1. a and d . Isolated follicles after using collagenase NB6; b and e . Isolated follicles after using Liberase DH; c and f . Isolated follicles after using collagenase IA; green: live follicles; red: dead follicles; scale bar = 100 μm
Article Snippet: They underwent mechanical and enzymatic dissociation using either collagenase IA routinely used in our laboratory (200 IU/ml final, Sigma Aldrich), a GMP grade purified collagenase NB6 (200 IU/ml final, Serva Electrophoresis GMbH; Coger, France), or Liberase DH (0.28 Wünsch U/ml, Roche, France).
Techniques: Viability Assay, Isolation, IA