Journal: Scientific Reports
Article Title: eIF5A inhibition influences T cell dynamics in the pancreatic microenvironment of the humanized mouse model of Type 1 Diabetes
Figure Lengend Snippet: Differential expression of elF5A, Hypusinated elF5A and DHPS in CD4 and CD8 T cells. (A) Western blot of CD4 and CD8 lymphocytes isolated from pancreatic lymph nodes (LN) and spleen (S). All the bands present in male/female samples were extracted from the respective single blot with the same exposure time. (B) DHPS, Hypusine, and elF5A were less expressed in CD8 T cells of pancreatic lymph nodes whereas the expression of these proteins were higher at splenic CD8. Note: elF5A protein expression was significant (P
Article Snippet: The blot was blocked with 5% nonfat dry milk/3% bovine serum albumin (BSA) and further incubated with primary antibodies against mice DHPS (Cat#ab202133, Abcam, Cambridge, United Kingdom), Hypusinated elF5A (Cat#ABS1064, Millipore Sigma, Burlington, MA), elF5A (Cat#ab137561, Abcam, Cambridge, United Kingdom) and α/β-Tubulin (Cat#2148 S, Cell Signaling, Danvers, MA).
Techniques: Expressing, Western Blot, Isolation