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  • 88
    Thermo Fisher dh5αmcr
    (A) Expression of the PvpA protein in E. coli under T7 promoter control. E. coli <t>DH5αMCR</t> cells harboring the recombinant plasmid pKPV.2 were subjected to selective induction of the T7 promoter. An SDS-PAGE autoradiograph of [ 35 S]methionine-labeled proteins expressed in E. coli (left panel) or a Western blot analysis of the same proteins as depicted in the left panel with MAb 1E5 (right panel) is shown. Cells in both panels were used without induction (lanes 1), with induction of the T7 promoter (lanes 2), or with induction in the presence of rifampin (lanes 3). A labeled arrow indicates the recombinant PvpA 55-kDa product expressed in E. coli . (B) Amino acid sequence and structural features of the PvpA protein from M. gallisepticum strain R. Amino acid residues are numbered on the right. A broken line marks the putative PvpA signal peptide. Labeled arrows show the position and direction of two directly repeated amino acid sequences (DR-1 and DR-2). A repeated motif consisting of four amino acids, PRPX (X is Met, Gln, or Asn), which appears 14 times within the PvpA C-terminal region is highlighted by boldface. The fourth codon, within a tract of five glutamine residues and in which a nonsense mutation has occurred, is marked by an arrowhead. Sixty proline residues within the C-terminal region are marked by asterisks.
    Dh5αmcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher escherichia coli dh5αmcr
    (A) Expression of the PvpA protein in E. coli under T7 promoter control. E. coli <t>DH5αMCR</t> cells harboring the recombinant plasmid pKPV.2 were subjected to selective induction of the T7 promoter. An SDS-PAGE autoradiograph of [ 35 S]methionine-labeled proteins expressed in E. coli (left panel) or a Western blot analysis of the same proteins as depicted in the left panel with MAb 1E5 (right panel) is shown. Cells in both panels were used without induction (lanes 1), with induction of the T7 promoter (lanes 2), or with induction in the presence of rifampin (lanes 3). A labeled arrow indicates the recombinant PvpA 55-kDa product expressed in E. coli . (B) Amino acid sequence and structural features of the PvpA protein from M. gallisepticum strain R. Amino acid residues are numbered on the right. A broken line marks the putative PvpA signal peptide. Labeled arrows show the position and direction of two directly repeated amino acid sequences (DR-1 and DR-2). A repeated motif consisting of four amino acids, PRPX (X is Met, Gln, or Asn), which appears 14 times within the PvpA C-terminal region is highlighted by boldface. The fourth codon, within a tract of five glutamine residues and in which a nonsense mutation has occurred, is marked by an arrowhead. Sixty proline residues within the C-terminal region are marked by asterisks.
    Escherichia Coli Dh5αmcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Thermo Fisher dh5αmcr competent cells
    (A) Expression of the PvpA protein in E. coli under T7 promoter control. E. coli <t>DH5αMCR</t> cells harboring the recombinant plasmid pKPV.2 were subjected to selective induction of the T7 promoter. An SDS-PAGE autoradiograph of [ 35 S]methionine-labeled proteins expressed in E. coli (left panel) or a Western blot analysis of the same proteins as depicted in the left panel with MAb 1E5 (right panel) is shown. Cells in both panels were used without induction (lanes 1), with induction of the T7 promoter (lanes 2), or with induction in the presence of rifampin (lanes 3). A labeled arrow indicates the recombinant PvpA 55-kDa product expressed in E. coli . (B) Amino acid sequence and structural features of the PvpA protein from M. gallisepticum strain R. Amino acid residues are numbered on the right. A broken line marks the putative PvpA signal peptide. Labeled arrows show the position and direction of two directly repeated amino acid sequences (DR-1 and DR-2). A repeated motif consisting of four amino acids, PRPX (X is Met, Gln, or Asn), which appears 14 times within the PvpA C-terminal region is highlighted by boldface. The fourth codon, within a tract of five glutamine residues and in which a nonsense mutation has occurred, is marked by an arrowhead. Sixty proline residues within the C-terminal region are marked by asterisks.
    Dh5αmcr Competent Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher reca muntant escherichia coli strains dh5αmcr
    (A) Expression of the PvpA protein in E. coli under T7 promoter control. E. coli <t>DH5αMCR</t> cells harboring the recombinant plasmid pKPV.2 were subjected to selective induction of the T7 promoter. An SDS-PAGE autoradiograph of [ 35 S]methionine-labeled proteins expressed in E. coli (left panel) or a Western blot analysis of the same proteins as depicted in the left panel with MAb 1E5 (right panel) is shown. Cells in both panels were used without induction (lanes 1), with induction of the T7 promoter (lanes 2), or with induction in the presence of rifampin (lanes 3). A labeled arrow indicates the recombinant PvpA 55-kDa product expressed in E. coli . (B) Amino acid sequence and structural features of the PvpA protein from M. gallisepticum strain R. Amino acid residues are numbered on the right. A broken line marks the putative PvpA signal peptide. Labeled arrows show the position and direction of two directly repeated amino acid sequences (DR-1 and DR-2). A repeated motif consisting of four amino acids, PRPX (X is Met, Gln, or Asn), which appears 14 times within the PvpA C-terminal region is highlighted by boldface. The fourth codon, within a tract of five glutamine residues and in which a nonsense mutation has occurred, is marked by an arrowhead. Sixty proline residues within the C-terminal region are marked by asterisks.
    Reca Muntant Escherichia Coli Strains Dh5αmcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    (A) Expression of the PvpA protein in E. coli under T7 promoter control. E. coli DH5αMCR cells harboring the recombinant plasmid pKPV.2 were subjected to selective induction of the T7 promoter. An SDS-PAGE autoradiograph of [ 35 S]methionine-labeled proteins expressed in E. coli (left panel) or a Western blot analysis of the same proteins as depicted in the left panel with MAb 1E5 (right panel) is shown. Cells in both panels were used without induction (lanes 1), with induction of the T7 promoter (lanes 2), or with induction in the presence of rifampin (lanes 3). A labeled arrow indicates the recombinant PvpA 55-kDa product expressed in E. coli . (B) Amino acid sequence and structural features of the PvpA protein from M. gallisepticum strain R. Amino acid residues are numbered on the right. A broken line marks the putative PvpA signal peptide. Labeled arrows show the position and direction of two directly repeated amino acid sequences (DR-1 and DR-2). A repeated motif consisting of four amino acids, PRPX (X is Met, Gln, or Asn), which appears 14 times within the PvpA C-terminal region is highlighted by boldface. The fourth codon, within a tract of five glutamine residues and in which a nonsense mutation has occurred, is marked by an arrowhead. Sixty proline residues within the C-terminal region are marked by asterisks.

    Journal: Infection and Immunity

    Article Title: Molecular Characterization of the Mycoplasma gallisepticum pvpA Gene Which Encodes a Putative Variable Cytadhesin Protein

    doi:

    Figure Lengend Snippet: (A) Expression of the PvpA protein in E. coli under T7 promoter control. E. coli DH5αMCR cells harboring the recombinant plasmid pKPV.2 were subjected to selective induction of the T7 promoter. An SDS-PAGE autoradiograph of [ 35 S]methionine-labeled proteins expressed in E. coli (left panel) or a Western blot analysis of the same proteins as depicted in the left panel with MAb 1E5 (right panel) is shown. Cells in both panels were used without induction (lanes 1), with induction of the T7 promoter (lanes 2), or with induction in the presence of rifampin (lanes 3). A labeled arrow indicates the recombinant PvpA 55-kDa product expressed in E. coli . (B) Amino acid sequence and structural features of the PvpA protein from M. gallisepticum strain R. Amino acid residues are numbered on the right. A broken line marks the putative PvpA signal peptide. Labeled arrows show the position and direction of two directly repeated amino acid sequences (DR-1 and DR-2). A repeated motif consisting of four amino acids, PRPX (X is Met, Gln, or Asn), which appears 14 times within the PvpA C-terminal region is highlighted by boldface. The fourth codon, within a tract of five glutamine residues and in which a nonsense mutation has occurred, is marked by an arrowhead. Sixty proline residues within the C-terminal region are marked by asterisks.

    Article Snippet: The Escherichia coli strains used were DH5αMCR (Gibco BRL Life Technologies, Inc., Gaithersburg, Md.) and Y1090 (Promega, Madison, Wis.).

    Techniques: Expressing, Recombinant, Plasmid Preparation, SDS Page, Autoradiography, Labeling, Western Blot, Sequencing, Mutagenesis