Journal: Pathogens

Article Title: CRISPR- cas3 of Salmonella Upregulates Bacterial Biofilm Formation and Virulence to Host Cells by Targeting Quorum-Sensing Systems

doi: 10.3390/pathogens9010053

Figure Lengend Snippet: Comparison of the expression level of some key genes acquired from RNA-Seq and RT-qPCR, including cas operon genes ( A ), lsr ( LuxS-regulated ) operon genes ( B ), and some of the SPI-1-T3SS genes ( Salmonella pathogenicity island 1 genes related to the type three secretion system) ( C ).

Article Snippet: E. coli DH 5α competence cells were purchased from Beijing TransGen Biotech Co., Ltd. (Beijing, China).

Techniques: Comparison, Expressing, RNA Sequencing, Quantitative RT-PCR

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Targeting the Nonmevalonate Pathway in Burkholderia cenocepacia Increases Susceptibility to Certain β-Lactam Antibiotics

doi: 10.1128/AAC.02607-17

Figure Lengend Snippet: Bacterial strains and plasmids

Article Snippet: DH5α λpir , λpir, for propagation of suicide plasmid pSC200 and its derivatives , Biomedal.

Techniques: Plasmid Preparation, Control, Transformation Assay

Journal: Microbial Cell Factories

Article Title: Enhanced fluorescent properties of an OmpT site deleted mutant of Green Fluorescent Protein

doi: 10.1186/1475-2859-9-26

Figure Lengend Snippet: GFP expression and activity in two different types of E. coli hosts . (A) GFP expression from pBAD24M-GFPuv clone at 37°C. Lane 1: Protein molecular weight marker (14 to 97 kDa); lane 2, GFP expression in DH5α cells; lane 3, GFP expression in BL21 cells. (B) GFP fluorescence units of pBAD24M-GFPuv at 37°C. Sample 1, pBAD24M-GFPuv in DH5α cells; Sample 2, pBAD24M-pGFPuv in BL21 cells.

Article Snippet: Escherichia coli K12 DH5α, LE392, JM109 were purchased from Bangalore Genei Pvt Ltd, Bangalore, India while BL21 was from Stratagene, USA respectively.

Techniques: Expressing, Activity Assay, Molecular Weight, Marker, Fluorescence

Journal: Microbial Cell Factories

Article Title: Enhanced fluorescent properties of an OmpT site deleted mutant of Green Fluorescent Protein

doi: 10.1186/1475-2859-9-26

Figure Lengend Snippet: Effect of induction temperature on GFP expression and fluorescence from various GFP constructs . (A) SDS-PAGE profile of GFP expressed from pBAD24M-GFP (WT), SDM I and SDM II clones in E. coli K12 DH5α cells at 30 and 37°C. Note the enhanced expression of GFP at 30°C for all the clones in comparison to 37°C. Lanes 1, 3, 5 represent GFP expressed at 30°C from WT, SDM I and SDM II clones respectively while lanes 2, 4, 6 represent GFP expressed from WT, SDM I and SDM II at 37°C respectively. (B) Fluorescence activity units of GFP mutants. Sample 1: pBAD24M-GFPuv; Sample 2: SDM I; Sample 3: SDM II. Empty bars represent 30°C induced samples while filled bars represent 37°C induced samples. GFP activity units obtained at 37°C was calculated taking GFP activity units achieved at 30°C as 100%.

Article Snippet: Escherichia coli K12 DH5α, LE392, JM109 were purchased from Bangalore Genei Pvt Ltd, Bangalore, India while BL21 was from Stratagene, USA respectively.

Techniques: Expressing, Fluorescence, Construct, SDS Page, Clone Assay, Comparison, Activity Assay

Journal: Microbial Cell Factories

Article Title: Enhanced fluorescent properties of an OmpT site deleted mutant of Green Fluorescent Protein

doi: 10.1186/1475-2859-9-26

Figure Lengend Snippet: Fluorescence data of GFP WT and SDM II in BL21, DH5α, JM109 and LE392 at 37°C . The relative fluorescence values with ± SEM are presented with the fluorescence of GFP obtained in BL21 defined as 100 (arbitrary unit). Sample 1: BL21; sample 2: DH5α; sample 3: JM109; sample 4: LE392. Filled bars represent WT while the empty bars represent SDM II GFP mutant.

Article Snippet: Escherichia coli K12 DH5α, LE392, JM109 were purchased from Bangalore Genei Pvt Ltd, Bangalore, India while BL21 was from Stratagene, USA respectively.

Techniques: Fluorescence, Mutagenesis

Journal: Microbial Cell Factories

Article Title: Enhanced fluorescent properties of an OmpT site deleted mutant of Green Fluorescent Protein

doi: 10.1186/1475-2859-9-26

Figure Lengend Snippet: Fermentation studies of GFP expression . (A) Growth profiles of pBAD24M-GFP, SDM I and SDM II at 30°C in DH5α cells in a bioreactor. The arrow indicates the point of induction. The growth was monitored at A 600 nm and DO levels were indicative of effective viable cells. (B) Fluorescence of the GFP samples of harvest samples of pBAD24M-GFPuv (lane 1), SDM I (lane 2) and SDM II (lane 3) under uv light after loading on SDS-PAGE without sample boiling. Arrow indicates the fluorescent protein.

Article Snippet: Escherichia coli K12 DH5α, LE392, JM109 were purchased from Bangalore Genei Pvt Ltd, Bangalore, India while BL21 was from Stratagene, USA respectively.

Techniques: Expressing, Fluorescence, SDS Page

Journal: Microbial Cell Factories

Article Title: Enhanced fluorescent properties of an OmpT site deleted mutant of Green Fluorescent Protein

doi: 10.1186/1475-2859-9-26

Figure Lengend Snippet: Quantitation of active GFP molecules obtained from different clones of GFPuv from pBAD24M-GFP constructs in DH5α cell free extracts at 30°C.

Article Snippet: Escherichia coli K12 DH5α, LE392, JM109 were purchased from Bangalore Genei Pvt Ltd, Bangalore, India while BL21 was from Stratagene, USA respectively.

Techniques: Quantitation Assay, Clone Assay, Construct, Activity Assay