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  • 96
    Thermo Fisher dh5 α competent cells
    Dh5 α Competent Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 98 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa dh 5α
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    tiangen biotech co dh 5α
    Dh 5α, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 92/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Toyobo dh 5α
    Selection of Rzs that suppress Tm-induced cell death from a randomized library. ( A ) Schematic representation of the selection system. ( B ) Number of transformants after each round of selection. Vectors isolated from surviving cells were introduced into <t>DH5α.</t> ( C ) Individual Rzs, selected from the randomized library, were examined for their ability to protect cells against Tm-mediated apoptosis. SK-N-SH cells that expressed selected Rzs were exposed to 2 μg/ml Tm for 24 h. Apoptosis was assessed by TUNEL staining. ( D ) The activation of caspase-3 depended on the activation of PKR. Cells were treated with Tm for 24 h or untreated (control), and then caspase-3 activity was measured. Cells treated with z-VAD-fmk were examined as controls.
    Dh 5α, supplied by Toyobo, used in various techniques. Bioz Stars score: 88/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher max efficient dh 5α competent cells
    Selection of Rzs that suppress Tm-induced cell death from a randomized library. ( A ) Schematic representation of the selection system. ( B ) Number of transformants after each round of selection. Vectors isolated from surviving cells were introduced into <t>DH5α.</t> ( C ) Individual Rzs, selected from the randomized library, were examined for their ability to protect cells against Tm-mediated apoptosis. SK-N-SH cells that expressed selected Rzs were exposed to 2 μg/ml Tm for 24 h. Apoptosis was assessed by TUNEL staining. ( D ) The activation of caspase-3 depended on the activation of PKR. Cells were treated with Tm for 24 h or untreated (control), and then caspase-3 activity was measured. Cells treated with z-VAD-fmk were examined as controls.
    Max Efficient Dh 5α Competent Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Genentech dh5α
    Selection of Rzs that suppress Tm-induced cell death from a randomized library. ( A ) Schematic representation of the selection system. ( B ) Number of transformants after each round of selection. Vectors isolated from surviving cells were introduced into <t>DH5α.</t> ( C ) Individual Rzs, selected from the randomized library, were examined for their ability to protect cells against Tm-mediated apoptosis. SK-N-SH cells that expressed selected Rzs were exposed to 2 μg/ml Tm for 24 h. Apoptosis was assessed by TUNEL staining. ( D ) The activation of caspase-3 depended on the activation of PKR. Cells were treated with Tm for 24 h or untreated (control), and then caspase-3 activity was measured. Cells treated with z-VAD-fmk were examined as controls.
    Dh5α, supplied by Genentech, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene dh5α
    Identification of a small molecule that inhibits RctB-dependent replication. A) Schematic of the RctB-dependent oriCII -bearing plasmid, pYB289, used in the high throughput screen for small molecule inhibitors of RctB. B) Structure of vibrepin, one of the compounds identified in the screen. C–G) growth curves of E. coli <t>DH5α</t> harboring no plasmid, pYB289 (encoding wt RctB), pYB340 (encoding RctB[L365I]), pYB344 (encoding RctB [P516Q]) and pWSK129 (a non- oriCII -based plasmid). Thick black lines represent growth in the presence of vibrepin (16 µg/ml) and gray lines represent growth in the presence of DMSO. Representative growth curves (average of triplicate wells from a plate reader) from 3 or more independent experiments are presented. The higher initial optical density of the cultures containing vibrepin is due to the incomplete solubility of this compound in LB media at 16 µg/ml. This is apparent in H) which shows the optical density generated by vibrepin (thick line), or DMSO (thin gray line) without the addition of cells.
    Dh5α, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher dh5α
    E. coli <t>DH5α</t> cells, an E. coli K-12 strain, expressing the wild-type E. coli TyrRS from plasmid pEcYS (filled circles) and those expressing iodoTyrRS- ec from plasmid pEcIYS (open circles) were grown in the presence of 3-bromo- l -tyrosine (0.5 mg/ml) ( A ) and 3-azido- l -tyrosine (0.3 mg/ml) ( B ). FT3 cells expressing iodoTyrRS- ec were grown in the absence (filled circles) and presence (open circles) of 3-bromo- l -tyrosine ( C ). The growth was monitored upon the addition of the non-natural amino acid to the growth media.
    Dh5α, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 823 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher electromax dh5 α e cells
    E. coli <t>DH5α</t> cells, an E. coli K-12 strain, expressing the wild-type E. coli TyrRS from plasmid pEcYS (filled circles) and those expressing iodoTyrRS- ec from plasmid pEcIYS (open circles) were grown in the presence of 3-bromo- l -tyrosine (0.5 mg/ml) ( A ) and 3-azido- l -tyrosine (0.3 mg/ml) ( B ). FT3 cells expressing iodoTyrRS- ec were grown in the absence (filled circles) and presence (open circles) of 3-bromo- l -tyrosine ( C ). The growth was monitored upon the addition of the non-natural amino acid to the growth media.
    Electromax Dh5 α E Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies dh5α
    E. coli <t>DH5α</t> cells, an E. coli K-12 strain, expressing the wild-type E. coli TyrRS from plasmid pEcYS (filled circles) and those expressing iodoTyrRS- ec from plasmid pEcIYS (open circles) were grown in the presence of 3-bromo- l -tyrosine (0.5 mg/ml) ( A ) and 3-azido- l -tyrosine (0.3 mg/ml) ( B ). FT3 cells expressing iodoTyrRS- ec were grown in the absence (filled circles) and presence (open circles) of 3-bromo- l -tyrosine ( C ). The growth was monitored upon the addition of the non-natural amino acid to the growth media.
    Dh5α, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore dh5α
    E. coli <t>DH5α</t> cells, an E. coli K-12 strain, expressing the wild-type E. coli TyrRS from plasmid pEcYS (filled circles) and those expressing iodoTyrRS- ec from plasmid pEcIYS (open circles) were grown in the presence of 3-bromo- l -tyrosine (0.5 mg/ml) ( A ) and 3-azido- l -tyrosine (0.3 mg/ml) ( B ). FT3 cells expressing iodoTyrRS- ec were grown in the absence (filled circles) and presence (open circles) of 3-bromo- l -tyrosine ( C ). The growth was monitored upon the addition of the non-natural amino acid to the growth media.
    Dh5α, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 133 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    RBC Bioscience dh5α
    E. coli <t>DH5α</t> cells, an E. coli K-12 strain, expressing the wild-type E. coli TyrRS from plasmid pEcYS (filled circles) and those expressing iodoTyrRS- ec from plasmid pEcIYS (open circles) were grown in the presence of 3-bromo- l -tyrosine (0.5 mg/ml) ( A ) and 3-azido- l -tyrosine (0.3 mg/ml) ( B ). FT3 cells expressing iodoTyrRS- ec were grown in the absence (filled circles) and presence (open circles) of 3-bromo- l -tyrosine ( C ). The growth was monitored upon the addition of the non-natural amino acid to the growth media.
    Dh5α, supplied by RBC Bioscience, used in various techniques. Bioz Stars score: 91/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck & Co dh5α
    E. coli <t>DH5α</t> cells, an E. coli K-12 strain, expressing the wild-type E. coli TyrRS from plasmid pEcYS (filled circles) and those expressing iodoTyrRS- ec from plasmid pEcIYS (open circles) were grown in the presence of 3-bromo- l -tyrosine (0.5 mg/ml) ( A ) and 3-azido- l -tyrosine (0.3 mg/ml) ( B ). FT3 cells expressing iodoTyrRS- ec were grown in the absence (filled circles) and presence (open circles) of 3-bromo- l -tyrosine ( C ). The growth was monitored upon the addition of the non-natural amino acid to the growth media.
    Dh5α, supplied by Merck & Co, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Beyotime dh5α
    E. coli <t>DH5α</t> cells, an E. coli K-12 strain, expressing the wild-type E. coli TyrRS from plasmid pEcYS (filled circles) and those expressing iodoTyrRS- ec from plasmid pEcIYS (open circles) were grown in the presence of 3-bromo- l -tyrosine (0.5 mg/ml) ( A ) and 3-azido- l -tyrosine (0.3 mg/ml) ( B ). FT3 cells expressing iodoTyrRS- ec were grown in the absence (filled circles) and presence (open circles) of 3-bromo- l -tyrosine ( C ). The growth was monitored upon the addition of the non-natural amino acid to the growth media.
    Dh5α, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson dh5α
    E. coli <t>DH5α</t> cells, an E. coli K-12 strain, expressing the wild-type E. coli TyrRS from plasmid pEcYS (filled circles) and those expressing iodoTyrRS- ec from plasmid pEcIYS (open circles) were grown in the presence of 3-bromo- l -tyrosine (0.5 mg/ml) ( A ) and 3-azido- l -tyrosine (0.3 mg/ml) ( B ). FT3 cells expressing iodoTyrRS- ec were grown in the absence (filled circles) and presence (open circles) of 3-bromo- l -tyrosine ( C ). The growth was monitored upon the addition of the non-natural amino acid to the growth media.
    Dh5α, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Genewiz dh5α
    E. coli <t>DH5α</t> cells, an E. coli K-12 strain, expressing the wild-type E. coli TyrRS from plasmid pEcYS (filled circles) and those expressing iodoTyrRS- ec from plasmid pEcIYS (open circles) were grown in the presence of 3-bromo- l -tyrosine (0.5 mg/ml) ( A ) and 3-azido- l -tyrosine (0.3 mg/ml) ( B ). FT3 cells expressing iodoTyrRS- ec were grown in the absence (filled circles) and presence (open circles) of 3-bromo- l -tyrosine ( C ). The growth was monitored upon the addition of the non-natural amino acid to the growth media.
    Dh5α, supplied by Genewiz, used in various techniques. Bioz Stars score: 90/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Sangon Biotech dh5α
    E. coli <t>DH5α</t> cells, an E. coli K-12 strain, expressing the wild-type E. coli TyrRS from plasmid pEcYS (filled circles) and those expressing iodoTyrRS- ec from plasmid pEcIYS (open circles) were grown in the presence of 3-bromo- l -tyrosine (0.5 mg/ml) ( A ) and 3-azido- l -tyrosine (0.3 mg/ml) ( B ). FT3 cells expressing iodoTyrRS- ec were grown in the absence (filled circles) and presence (open circles) of 3-bromo- l -tyrosine ( C ). The growth was monitored upon the addition of the non-natural amino acid to the growth media.
    Dh5α, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 90/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Selection of Rzs that suppress Tm-induced cell death from a randomized library. ( A ) Schematic representation of the selection system. ( B ) Number of transformants after each round of selection. Vectors isolated from surviving cells were introduced into DH5α. ( C ) Individual Rzs, selected from the randomized library, were examined for their ability to protect cells against Tm-mediated apoptosis. SK-N-SH cells that expressed selected Rzs were exposed to 2 μg/ml Tm for 24 h. Apoptosis was assessed by TUNEL staining. ( D ) The activation of caspase-3 depended on the activation of PKR. Cells were treated with Tm for 24 h or untreated (control), and then caspase-3 activity was measured. Cells treated with z-VAD-fmk were examined as controls.

    Journal: The EMBO Journal

    Article Title: An RNA-dependent protein kinase is involved in tunicamycin-induced apoptosis and Alzheimer's disease

    doi: 10.1038/sj.emboj.7600049

    Figure Lengend Snippet: Selection of Rzs that suppress Tm-induced cell death from a randomized library. ( A ) Schematic representation of the selection system. ( B ) Number of transformants after each round of selection. Vectors isolated from surviving cells were introduced into DH5α. ( C ) Individual Rzs, selected from the randomized library, were examined for their ability to protect cells against Tm-mediated apoptosis. SK-N-SH cells that expressed selected Rzs were exposed to 2 μg/ml Tm for 24 h. Apoptosis was assessed by TUNEL staining. ( D ) The activation of caspase-3 depended on the activation of PKR. Cells were treated with Tm for 24 h or untreated (control), and then caspase-3 activity was measured. Cells treated with z-VAD-fmk were examined as controls.

    Article Snippet: For the first screening, transfected cells were exposed to Tm for 24 h. For the second screening, cells were transfected with candidate plasmids from the first screening and collected after incubation with Tm for 48 h. Expression vectors harboring Rzs were isolated from surviving cells and used to transform DH5α (TOYOBO, Tokyo, Japan).

    Techniques: Selection, Isolation, TUNEL Assay, Staining, Activation Assay, Activity Assay

    Identification of a small molecule that inhibits RctB-dependent replication. A) Schematic of the RctB-dependent oriCII -bearing plasmid, pYB289, used in the high throughput screen for small molecule inhibitors of RctB. B) Structure of vibrepin, one of the compounds identified in the screen. C–G) growth curves of E. coli DH5α harboring no plasmid, pYB289 (encoding wt RctB), pYB340 (encoding RctB[L365I]), pYB344 (encoding RctB [P516Q]) and pWSK129 (a non- oriCII -based plasmid). Thick black lines represent growth in the presence of vibrepin (16 µg/ml) and gray lines represent growth in the presence of DMSO. Representative growth curves (average of triplicate wells from a plate reader) from 3 or more independent experiments are presented. The higher initial optical density of the cultures containing vibrepin is due to the incomplete solubility of this compound in LB media at 16 µg/ml. This is apparent in H) which shows the optical density generated by vibrepin (thick line), or DMSO (thin gray line) without the addition of cells.

    Journal: PLoS Pathogens

    Article Title: Targeting the Replication Initiator of the Second Vibrio Chromosome: Towards Generation of Vibrionaceae-Specific Antimicrobial Agents

    doi: 10.1371/journal.ppat.1000663

    Figure Lengend Snippet: Identification of a small molecule that inhibits RctB-dependent replication. A) Schematic of the RctB-dependent oriCII -bearing plasmid, pYB289, used in the high throughput screen for small molecule inhibitors of RctB. B) Structure of vibrepin, one of the compounds identified in the screen. C–G) growth curves of E. coli DH5α harboring no plasmid, pYB289 (encoding wt RctB), pYB340 (encoding RctB[L365I]), pYB344 (encoding RctB [P516Q]) and pWSK129 (a non- oriCII -based plasmid). Thick black lines represent growth in the presence of vibrepin (16 µg/ml) and gray lines represent growth in the presence of DMSO. Representative growth curves (average of triplicate wells from a plate reader) from 3 or more independent experiments are presented. The higher initial optical density of the cultures containing vibrepin is due to the incomplete solubility of this compound in LB media at 16 µg/ml. This is apparent in H) which shows the optical density generated by vibrepin (thick line), or DMSO (thin gray line) without the addition of cells.

    Article Snippet: We tested the sensitivity of DH5α harboring several of these mutant oriCII plasmids to vibrepin and found that plasmids A-52 and A-57 containing rctB [L365I] and [P516Q] respectively confer resistance to the compound. rctA was deleted from A-52 and A-57, yielding pYB340 and pYB344 respectively, using the QuickChange XL Site Directed Mutagenesis Kit (Stratagene).

    Techniques: Plasmid Preparation, High Throughput Screening Assay, Solubility, Generated

    Influence of vibrepin on an E. coli strain bearing an RctB-dependent plasmid in the absence of kanamycin. A–C) DH5α (A), DH5α/pYB289 (B), or DH5α/pYB376 (C) were incubated in LB media in test tubes with either vibrepin (16 µg/ml, thick black lines) or DMSO (gray lines). OD 600 nm and colony forming units (CFU) were determined at the indicated times. Representative growth curves from 3 or more independent experiments are presented. D) The relative amount of the oriCII -based plasmid pYB289 in DH5α after 4 hr of treatment with vibrepin (16 µg/ml) or DMSO. The amount of pYB289 relative to chromosomal DNA was determined before (t = 0) and 4 hrs after treatment (t = 4) using Southern hybridization. The relative amount of pYB289 at t = 0 was set as 1; the mean and standard deviations after 4 hr were calculated from 3 independent experiments.

    Journal: PLoS Pathogens

    Article Title: Targeting the Replication Initiator of the Second Vibrio Chromosome: Towards Generation of Vibrionaceae-Specific Antimicrobial Agents

    doi: 10.1371/journal.ppat.1000663

    Figure Lengend Snippet: Influence of vibrepin on an E. coli strain bearing an RctB-dependent plasmid in the absence of kanamycin. A–C) DH5α (A), DH5α/pYB289 (B), or DH5α/pYB376 (C) were incubated in LB media in test tubes with either vibrepin (16 µg/ml, thick black lines) or DMSO (gray lines). OD 600 nm and colony forming units (CFU) were determined at the indicated times. Representative growth curves from 3 or more independent experiments are presented. D) The relative amount of the oriCII -based plasmid pYB289 in DH5α after 4 hr of treatment with vibrepin (16 µg/ml) or DMSO. The amount of pYB289 relative to chromosomal DNA was determined before (t = 0) and 4 hrs after treatment (t = 4) using Southern hybridization. The relative amount of pYB289 at t = 0 was set as 1; the mean and standard deviations after 4 hr were calculated from 3 independent experiments.

    Article Snippet: We tested the sensitivity of DH5α harboring several of these mutant oriCII plasmids to vibrepin and found that plasmids A-52 and A-57 containing rctB [L365I] and [P516Q] respectively confer resistance to the compound. rctA was deleted from A-52 and A-57, yielding pYB340 and pYB344 respectively, using the QuickChange XL Site Directed Mutagenesis Kit (Stratagene).

    Techniques: Plasmid Preparation, Incubation, Hybridization

    E. coli DH5α cells, an E. coli K-12 strain, expressing the wild-type E. coli TyrRS from plasmid pEcYS (filled circles) and those expressing iodoTyrRS- ec from plasmid pEcIYS (open circles) were grown in the presence of 3-bromo- l -tyrosine (0.5 mg/ml) ( A ) and 3-azido- l -tyrosine (0.3 mg/ml) ( B ). FT3 cells expressing iodoTyrRS- ec were grown in the absence (filled circles) and presence (open circles) of 3-bromo- l -tyrosine ( C ). The growth was monitored upon the addition of the non-natural amino acid to the growth media.

    Journal: Nucleic Acids Research

    Article Title: Functional replacement of the endogenous tyrosyl-tRNA synthetase-tRNATyr pair by the archaeal tyrosine pair in Escherichia coli for genetic code expansion

    doi: 10.1093/nar/gkq080

    Figure Lengend Snippet: E. coli DH5α cells, an E. coli K-12 strain, expressing the wild-type E. coli TyrRS from plasmid pEcYS (filled circles) and those expressing iodoTyrRS- ec from plasmid pEcIYS (open circles) were grown in the presence of 3-bromo- l -tyrosine (0.5 mg/ml) ( A ) and 3-azido- l -tyrosine (0.3 mg/ml) ( B ). FT3 cells expressing iodoTyrRS- ec were grown in the absence (filled circles) and presence (open circles) of 3-bromo- l -tyrosine ( C ). The growth was monitored upon the addition of the non-natural amino acid to the growth media.

    Article Snippet: Strains, growth conditions, growth rate, reagents and non-natural amino acids Escherichia coli strains TOP10, BL21(DE3) and DH5α were purchased from Invitrogen, Novagen and Toyobo (Tokyo, Japan), respectively.

    Techniques: Expressing, Plasmid Preparation