dh10b Thermo Fisher Search Results


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  • 90
    Thermo Fisher electro max dh10btm cells
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    Annexin V staining demonstrates the apoptotic effect of bacterially delivered violacein at a MOI of 25 at 10 h post-infection. ( a ) 7% of HeLa S3 cells treated with a control invasive <t>DH10B</t> showed annexin V staining (right panel); ( b ) 33% of HeLa S3 cells treated with bacterially delivered violacein showed annexin V staining. Scale bars represent 100 μm.
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    Annexin V staining demonstrates the apoptotic effect of bacterially delivered violacein at a MOI of 25 at 10 h post-infection. ( a ) 7% of HeLa S3 cells treated with a control invasive <t>DH10B</t> showed annexin V staining (right panel); ( b ) 33% of HeLa S3 cells treated with bacterially delivered violacein showed annexin V staining. Scale bars represent 100 μm.
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    Annexin V staining demonstrates the apoptotic effect of bacterially delivered violacein at a MOI of 25 at 10 h post-infection. ( a ) 7% of HeLa S3 cells treated with a control invasive <t>DH10B</t> showed annexin V staining (right panel); ( b ) 33% of HeLa S3 cells treated with bacterially delivered violacein showed annexin V staining. Scale bars represent 100 μm.
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    Annexin V staining demonstrates the apoptotic effect of bacterially delivered violacein at a MOI of 25 at 10 h post-infection. ( a ) 7% of HeLa S3 cells treated with a control invasive <t>DH10B</t> showed annexin V staining (right panel); ( b ) 33% of HeLa S3 cells treated with bacterially delivered violacein showed annexin V staining. Scale bars represent 100 μm.
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    Annexin V staining demonstrates the apoptotic effect of bacterially delivered violacein at a MOI of 25 at 10 h post-infection. ( a ) 7% of HeLa S3 cells treated with a control invasive <t>DH10B</t> showed annexin V staining (right panel); ( b ) 33% of HeLa S3 cells treated with bacterially delivered violacein showed annexin V staining. Scale bars represent 100 μm.
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    Annexin V staining demonstrates the apoptotic effect of bacterially delivered violacein at a MOI of 25 at 10 h post-infection. ( a ) 7% of HeLa S3 cells treated with a control invasive <t>DH10B</t> showed annexin V staining (right panel); ( b ) 33% of HeLa S3 cells treated with bacterially delivered violacein showed annexin V staining. Scale bars represent 100 μm.
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    Annexin V staining demonstrates the apoptotic effect of bacterially delivered violacein at a MOI of 25 at 10 h post-infection. ( a ) 7% of HeLa S3 cells treated with a control invasive <t>DH10B</t> showed annexin V staining (right panel); ( b ) 33% of HeLa S3 cells treated with bacterially delivered violacein showed annexin V staining. Scale bars represent 100 μm.
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    Annexin V staining demonstrates the apoptotic effect of bacterially delivered violacein at a MOI of 25 at 10 h post-infection. ( a ) 7% of HeLa S3 cells treated with a control invasive <t>DH10B</t> showed annexin V staining (right panel); ( b ) 33% of HeLa S3 cells treated with bacterially delivered violacein showed annexin V staining. Scale bars represent 100 μm.
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    Annexin V staining demonstrates the apoptotic effect of bacterially delivered violacein at a MOI of 25 at 10 h post-infection. ( a ) 7% of HeLa S3 cells treated with a control invasive <t>DH10B</t> showed annexin V staining (right panel); ( b ) 33% of HeLa S3 cells treated with bacterially delivered violacein showed annexin V staining. Scale bars represent 100 μm.
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    Annexin V staining demonstrates the apoptotic effect of bacterially delivered violacein at a MOI of 25 at 10 h post-infection. ( a ) 7% of HeLa S3 cells treated with a control invasive <t>DH10B</t> showed annexin V staining (right panel); ( b ) 33% of HeLa S3 cells treated with bacterially delivered violacein showed annexin V staining. Scale bars represent 100 μm.
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    Annexin V staining demonstrates the apoptotic effect of bacterially delivered violacein at a MOI of 25 at 10 h post-infection. ( a ) 7% of HeLa S3 cells treated with a control invasive <t>DH10B</t> showed annexin V staining (right panel); ( b ) 33% of HeLa S3 cells treated with bacterially delivered violacein showed annexin V staining. Scale bars represent 100 μm.
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    Annexin V staining demonstrates the apoptotic effect of bacterially delivered violacein at a MOI of 25 at 10 h post-infection. ( a ) 7% of HeLa S3 cells treated with a control invasive <t>DH10B</t> showed annexin V staining (right panel); ( b ) 33% of HeLa S3 cells treated with bacterially delivered violacein showed annexin V staining. Scale bars represent 100 μm.
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    Annexin V staining demonstrates the apoptotic effect of bacterially delivered violacein at a MOI of 25 at 10 h post-infection. ( a ) 7% of HeLa S3 cells treated with a control invasive <t>DH10B</t> showed annexin V staining (right panel); ( b ) 33% of HeLa S3 cells treated with bacterially delivered violacein showed annexin V staining. Scale bars represent 100 μm.
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    Annexin V staining demonstrates the apoptotic effect of bacterially delivered violacein at a MOI of 25 at 10 h post-infection. ( a ) 7% of HeLa S3 cells treated with a control invasive <t>DH10B</t> showed annexin V staining (right panel); ( b ) 33% of HeLa S3 cells treated with bacterially delivered violacein showed annexin V staining. Scale bars represent 100 μm.
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    Annexin V staining demonstrates the apoptotic effect of bacterially delivered violacein at a MOI of 25 at 10 h post-infection. ( a ) 7% of HeLa S3 cells treated with a control invasive <t>DH10B</t> showed annexin V staining (right panel); ( b ) 33% of HeLa S3 cells treated with bacterially delivered violacein showed annexin V staining. Scale bars represent 100 μm.
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    Annexin V staining demonstrates the apoptotic effect of bacterially delivered violacein at a MOI of 25 at 10 h post-infection. ( a ) 7% of HeLa S3 cells treated with a control invasive <t>DH10B</t> showed annexin V staining (right panel); ( b ) 33% of HeLa S3 cells treated with bacterially delivered violacein showed annexin V staining. Scale bars represent 100 μm.
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    Annexin V staining demonstrates the apoptotic effect of bacterially delivered violacein at a MOI of 25 at 10 h post-infection. ( a ) 7% of HeLa S3 cells treated with a control invasive <t>DH10B</t> showed annexin V staining (right panel); ( b ) 33% of HeLa S3 cells treated with bacterially delivered violacein showed annexin V staining. Scale bars represent 100 μm.
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    Annexin V staining demonstrates the apoptotic effect of bacterially delivered violacein at a MOI of 25 at 10 h post-infection. ( a ) 7% of HeLa S3 cells treated with a control invasive <t>DH10B</t> showed annexin V staining (right panel); ( b ) 33% of HeLa S3 cells treated with bacterially delivered violacein showed annexin V staining. Scale bars represent 100 μm.
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    mTn5 transposon mutagenesis using an IPTG-controlled conditional suicide plasmid. a Diagram of plasmid pSNC-mTn5. pSNC-mTn5 is a derivative of pMMB-repF that contains a Kan R -tagged mTn5, a lac promoter-controlled hyperactive transposase gene ( tnp H ), and a sacB counter selection marker (with its own promoter). OE and IE are outside and inside ends of the mTn5. b Plasmid and transposon retention frequencies in E. coli <t>DH10B.</t> A “+” symbol for IPTG indicates that the inducer was added to the liquid culture, and a “+” symbol for Suc, Cam, and Kan indicates that the chemicals were added to the plates. Black columns represent plasmid retention frequencies, and the blue column represents Tn5 retention frequency. Results were average of three independent experiments, and bars represent mean ± SD (*p
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    Growth curves of <t>DH10B</t> bacteria expressing wild-type Vpu (top) or the A18H mutant (middle and bottom) as a function of different rimantadine concentrations. The bottom panel is an expansion of the middle panel.
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    Growth curves of <t>DH10B</t> bacteria expressing wild-type Vpu (top) or the A18H mutant (middle and bottom) as a function of different rimantadine concentrations. The bottom panel is an expansion of the middle panel.
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    Growth curves of <t>DH10B</t> bacteria expressing wild-type Vpu (top) or the A18H mutant (middle and bottom) as a function of different rimantadine concentrations. The bottom panel is an expansion of the middle panel.
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    Growth curves of <t>DH10B</t> bacteria expressing wild-type Vpu (top) or the A18H mutant (middle and bottom) as a function of different rimantadine concentrations. The bottom panel is an expansion of the middle panel.
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    Growth curves of <t>DH10B</t> bacteria expressing wild-type Vpu (top) or the A18H mutant (middle and bottom) as a function of different rimantadine concentrations. The bottom panel is an expansion of the middle panel.
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    Growth curves of <t>DH10B</t> bacteria expressing wild-type Vpu (top) or the A18H mutant (middle and bottom) as a function of different rimantadine concentrations. The bottom panel is an expansion of the middle panel.
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    Growth curves of <t>DH10B</t> bacteria expressing wild-type Vpu (top) or the A18H mutant (middle and bottom) as a function of different rimantadine concentrations. The bottom panel is an expansion of the middle panel.
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    Image Search Results


    Annexin V staining demonstrates the apoptotic effect of bacterially delivered violacein at a MOI of 25 at 10 h post-infection. ( a ) 7% of HeLa S3 cells treated with a control invasive DH10B showed annexin V staining (right panel); ( b ) 33% of HeLa S3 cells treated with bacterially delivered violacein showed annexin V staining. Scale bars represent 100 μm.

    Journal: Micromachines

    Article Title: A Droplet Microfluidics Based Platform for Mining Metagenomic Libraries for Natural Compounds

    doi: 10.3390/mi8080230

    Figure Lengend Snippet: Annexin V staining demonstrates the apoptotic effect of bacterially delivered violacein at a MOI of 25 at 10 h post-infection. ( a ) 7% of HeLa S3 cells treated with a control invasive DH10B showed annexin V staining (right panel); ( b ) 33% of HeLa S3 cells treated with bacterially delivered violacein showed annexin V staining. Scale bars represent 100 μm.

    Article Snippet: We dosed a suspension of HeLa S3 cells with violacein producing invasive DH10B in droplets to confirm if a drug producing operon could be detected and at least partially enriched above background in one cycle of cell sorting.

    Techniques: Staining, Infection

    Site-directed transposition in human cells. ( A ) Schematic overview of plasmid-based assay for investigating site-directed transposition. Transposition was initiated by transfecting human HeLa cells with a plasmid encoding chimeric or unfused HSB5 transposase, together with a donor plasmid encoding a bleomycin-marked (zeo r ) transposon and a counter-selectable chloramphenicol-resistance (cam r ) gene. These plasmids were co-delivered with an ampicillin-resistant (amp r ) target plasmid containing five tandem DBD recognition sequences and allowed to undergo transposition. Low-molecular weight plasmid DNA fractions were isolated 2 days later and transformed into DH10B E. coli . Replication of the R6K origin-containing donor plasmid is strictly dependent on expression of the pir1 gene product, which is absent in this bacterial strain. Amp r /zeo r bacterial colonies were patched onto LB-cam r plates to screen for inter-plasmid transposition events specific for the target plasmid (i.e. cam s ). Both pooled and clonal amp r /zeo r /cam s populations of bacteria were amplified, plasmid DNA isolated and the locations of transposon insertions relative to the target sites determined by restriction site analyses and DNA sequence analyses, respectively. ( B ) Target plasmid features. Positions of BglI and BglII restriction endonuclease recognition sites are shown, as are the sizes for each resulting DNA fragment. ( C ) Southern blot analysis of targeted integration. For each experimental condition, 500 ng of plasmid DNA isolated from pooled amp r /zeo r /cam s bacterial colonies ( n = 43–51) was treated with BglI-BglII restriction enzymes. Samples were resolved on an agarose gel, transferred to nitrocellulose, hybridized to a 32 P-radiolabelled probe corresponding to the left SB transposon inverted repeat, and resulting bands visualized upon autoradiography. In one instance, excess E2C DNA-binding domain was co-expressed with transposase protein to determine whether associated proteins could inhibit SB target site DNA capture. The lower band intensity under each experimental condition represents a qualitative assessment of the relative frequency of transposition of the 1.35 kb zeo r -marked element into the 443 bp targeting window. ( D ) Targeted transposition frequencies. Recombinant target DNA molecules were isolated from individual amp r /zeo r /cam s colonies and sequenced using an internal transposon-specific primer. Bars denote the percentage of total integrations occurring within the 443-bp targeting window, whereas the numbers in parentheses denote the actual number of integrations analyzed in each group. ( E ) Flexing model for targeted transposition. Multiple changes in both DNA- and protein conformation are necessary to complete a fullcycle of transposition. A protein that remains too tightly bound to DNA, such as hE2C-L5-SB to the canonical e2c site, cannot efficiently catalyze these reactions. In the case of the mutant e2c site, however, only fingers 1 through 3 of hE2C-L5-SB retain the capacity for DNA-binding. This would essentially improve the flexibility of the transposase domain, which might enhance the acquisition and/or manipulation of neighboring target sites.

    Journal: Nucleic Acids Research

    Article Title: Site-directed transposon integration in human cells

    doi: 10.1093/nar/gkm089

    Figure Lengend Snippet: Site-directed transposition in human cells. ( A ) Schematic overview of plasmid-based assay for investigating site-directed transposition. Transposition was initiated by transfecting human HeLa cells with a plasmid encoding chimeric or unfused HSB5 transposase, together with a donor plasmid encoding a bleomycin-marked (zeo r ) transposon and a counter-selectable chloramphenicol-resistance (cam r ) gene. These plasmids were co-delivered with an ampicillin-resistant (amp r ) target plasmid containing five tandem DBD recognition sequences and allowed to undergo transposition. Low-molecular weight plasmid DNA fractions were isolated 2 days later and transformed into DH10B E. coli . Replication of the R6K origin-containing donor plasmid is strictly dependent on expression of the pir1 gene product, which is absent in this bacterial strain. Amp r /zeo r bacterial colonies were patched onto LB-cam r plates to screen for inter-plasmid transposition events specific for the target plasmid (i.e. cam s ). Both pooled and clonal amp r /zeo r /cam s populations of bacteria were amplified, plasmid DNA isolated and the locations of transposon insertions relative to the target sites determined by restriction site analyses and DNA sequence analyses, respectively. ( B ) Target plasmid features. Positions of BglI and BglII restriction endonuclease recognition sites are shown, as are the sizes for each resulting DNA fragment. ( C ) Southern blot analysis of targeted integration. For each experimental condition, 500 ng of plasmid DNA isolated from pooled amp r /zeo r /cam s bacterial colonies ( n = 43–51) was treated with BglI-BglII restriction enzymes. Samples were resolved on an agarose gel, transferred to nitrocellulose, hybridized to a 32 P-radiolabelled probe corresponding to the left SB transposon inverted repeat, and resulting bands visualized upon autoradiography. In one instance, excess E2C DNA-binding domain was co-expressed with transposase protein to determine whether associated proteins could inhibit SB target site DNA capture. The lower band intensity under each experimental condition represents a qualitative assessment of the relative frequency of transposition of the 1.35 kb zeo r -marked element into the 443 bp targeting window. ( D ) Targeted transposition frequencies. Recombinant target DNA molecules were isolated from individual amp r /zeo r /cam s colonies and sequenced using an internal transposon-specific primer. Bars denote the percentage of total integrations occurring within the 443-bp targeting window, whereas the numbers in parentheses denote the actual number of integrations analyzed in each group. ( E ) Flexing model for targeted transposition. Multiple changes in both DNA- and protein conformation are necessary to complete a fullcycle of transposition. A protein that remains too tightly bound to DNA, such as hE2C-L5-SB to the canonical e2c site, cannot efficiently catalyze these reactions. In the case of the mutant e2c site, however, only fingers 1 through 3 of hE2C-L5-SB retain the capacity for DNA-binding. This would essentially improve the flexibility of the transposase domain, which might enhance the acquisition and/or manipulation of neighboring target sites.

    Article Snippet: PCR products were ligated to the pGEM-T easy vector (Promega), transformed into DH10B cells (Invitrogen) and analyzed using the IR-1 sequencing primer.

    Techniques: Plasmid Preparation, Chick Chorioallantoic Membrane Assay, Molecular Weight, Isolation, Transformation Assay, Expressing, Amplification, Sequencing, Southern Blot, Agarose Gel Electrophoresis, Autoradiography, Binding Assay, Recombinant, Mutagenesis

    A typical chimeric-GFP solubility screening experiment. ( A ) white light image, ( B ) transilluminating UV (365 nm) image of an agar plate used to select GFP-positive colonies in the solubility screen. Electrocompetent DH10B cells were transformed with a

    Journal: Protein Science : A Publication of the Protein Society

    Article Title: A green fluorescent protein solubility screen in E. coli reveals domain boundaries of the GTP-binding domain in the P element transposase

    doi: 10.1002/pro.499

    Figure Lengend Snippet: A typical chimeric-GFP solubility screening experiment. ( A ) white light image, ( B ) transilluminating UV (365 nm) image of an agar plate used to select GFP-positive colonies in the solubility screen. Electrocompetent DH10B cells were transformed with a

    Article Snippet: 2–5 μL of each ligation was transformed into 40 μL electrocompetent DH10B cells (Invitrogen), diluted into 1 mL SOC medium (Invitrogen), then plated onto 5 selective agar plates.

    Techniques: Solubility, Transformation Assay

    Temperature-sensitivity of Escherichia coli strains transformed with bicistronic PB1-PA expression vectors. (A) Representation of the TaV2A sequences of 1a, 1a(Met), and 1 ∗ a(Met) ORF vectors. The N-terminal sequence of PA (downstream region) and modified TaV2A residues [1 ∗ a(Met), upstream region] are indicated in red (uppercase letters). A putative SD sequence is indicated in blue, and nucleotide substitutions in the 1 ∗ a(Met) ORF are indicated in red (lowercase letters). (B) Colony forming assay of transformants at three different temperatures. Mach1, DH10B, and HB101 strains of E. coli were transformed with original phCMV-1a(Met) or -1 ∗ a(Met) in which the putative SD sequence was disrupted by nucleotide substitution. After recovery in SOC medium, cells were spread on LB agar plates containing kanamycin and incubated at 25, 30, or 37°C for 24 h.

    Journal: Frontiers in Microbiology

    Article Title: Polycistronic Expression of the Influenza A Virus RNA-Dependent RNA Polymerase by Using the Thosea asigna Virus 2A-Like Self-Processing Sequence

    doi: 10.3389/fmicb.2016.00288

    Figure Lengend Snippet: Temperature-sensitivity of Escherichia coli strains transformed with bicistronic PB1-PA expression vectors. (A) Representation of the TaV2A sequences of 1a, 1a(Met), and 1 ∗ a(Met) ORF vectors. The N-terminal sequence of PA (downstream region) and modified TaV2A residues [1 ∗ a(Met), upstream region] are indicated in red (uppercase letters). A putative SD sequence is indicated in blue, and nucleotide substitutions in the 1 ∗ a(Met) ORF are indicated in red (lowercase letters). (B) Colony forming assay of transformants at three different temperatures. Mach1, DH10B, and HB101 strains of E. coli were transformed with original phCMV-1a(Met) or -1 ∗ a(Met) in which the putative SD sequence was disrupted by nucleotide substitution. After recovery in SOC medium, cells were spread on LB agar plates containing kanamycin and incubated at 25, 30, or 37°C for 24 h.

    Article Snippet: Escherichia coli Mach1 or DH10B strain (Life Technologies, USA) was routinely used for transformation with expression vectors.

    Techniques: Transformation Assay, Expressing, Sequencing, Modification, Incubation

    mTn5 transposon mutagenesis using an IPTG-controlled conditional suicide plasmid. a Diagram of plasmid pSNC-mTn5. pSNC-mTn5 is a derivative of pMMB-repF that contains a Kan R -tagged mTn5, a lac promoter-controlled hyperactive transposase gene ( tnp H ), and a sacB counter selection marker (with its own promoter). OE and IE are outside and inside ends of the mTn5. b Plasmid and transposon retention frequencies in E. coli DH10B. A “+” symbol for IPTG indicates that the inducer was added to the liquid culture, and a “+” symbol for Suc, Cam, and Kan indicates that the chemicals were added to the plates. Black columns represent plasmid retention frequencies, and the blue column represents Tn5 retention frequency. Results were average of three independent experiments, and bars represent mean ± SD (*p

    Journal: BMC Microbiology

    Article Title: Efficient transposon mutagenesis mediated by an IPTG-controlled conditional suicide plasmid

    doi: 10.1186/s12866-018-1319-0

    Figure Lengend Snippet: mTn5 transposon mutagenesis using an IPTG-controlled conditional suicide plasmid. a Diagram of plasmid pSNC-mTn5. pSNC-mTn5 is a derivative of pMMB-repF that contains a Kan R -tagged mTn5, a lac promoter-controlled hyperactive transposase gene ( tnp H ), and a sacB counter selection marker (with its own promoter). OE and IE are outside and inside ends of the mTn5. b Plasmid and transposon retention frequencies in E. coli DH10B. A “+” symbol for IPTG indicates that the inducer was added to the liquid culture, and a “+” symbol for Suc, Cam, and Kan indicates that the chemicals were added to the plates. Black columns represent plasmid retention frequencies, and the blue column represents Tn5 retention frequency. Results were average of three independent experiments, and bars represent mean ± SD (*p

    Article Snippet: E. coli DH10B was purchased from Invitrogen.

    Techniques: Mutagenesis, Plasmid Preparation, Selection, Marker, Chick Chorioallantoic Membrane Assay

    Generation of a P. aeruginosa insertion library with pSNC-mTn5ME. a Diagram of pSNC-mTn5ME, a derivative of pSNC-mTn5 that has MEs instead of OE and IE at the termini of mTn5. b Plasmid and transposon retention frequencies in E. coli DH10B. Results were average of three independent experiments, and bars represent mean ± SD (* p

    Journal: BMC Microbiology

    Article Title: Efficient transposon mutagenesis mediated by an IPTG-controlled conditional suicide plasmid

    doi: 10.1186/s12866-018-1319-0

    Figure Lengend Snippet: Generation of a P. aeruginosa insertion library with pSNC-mTn5ME. a Diagram of pSNC-mTn5ME, a derivative of pSNC-mTn5 that has MEs instead of OE and IE at the termini of mTn5. b Plasmid and transposon retention frequencies in E. coli DH10B. Results were average of three independent experiments, and bars represent mean ± SD (* p

    Article Snippet: E. coli DH10B was purchased from Invitrogen.

    Techniques: Plasmid Preparation

    IPTG-controlled conditional suicide plasmids. a Plasmid pMMB208 and its conditional-suicide derivatives. pMMB208 contains an RSF1010 oriV for replication and an oriT for conjugation. Genes repA , mobA / repB and repC encode proteins required for plasmid replication, and repF encodes a transcription repressor that binds promoter P4. pMMB208 also has Cam R and lacI Q genes and a P tac promoter. Plasmid pMMB- repF is a derivative of pMMB208 that has a second copy of the repF gene inserted downstream of P tac . Plasmids pMMB- repA K42A and pMMB- repA D139A have a dominant-negative repA mutant gene, either K42A or D139A, inserted downstream of P tac . b Amount of E. coli DH10B cells retaining the indicated plasmids after 24 h growth in the absence of antibiotics, either with or without IPTG induction. Results were average of three independent experiments, and bars represent mean ± SD (standard deviation). * p

    Journal: BMC Microbiology

    Article Title: Efficient transposon mutagenesis mediated by an IPTG-controlled conditional suicide plasmid

    doi: 10.1186/s12866-018-1319-0

    Figure Lengend Snippet: IPTG-controlled conditional suicide plasmids. a Plasmid pMMB208 and its conditional-suicide derivatives. pMMB208 contains an RSF1010 oriV for replication and an oriT for conjugation. Genes repA , mobA / repB and repC encode proteins required for plasmid replication, and repF encodes a transcription repressor that binds promoter P4. pMMB208 also has Cam R and lacI Q genes and a P tac promoter. Plasmid pMMB- repF is a derivative of pMMB208 that has a second copy of the repF gene inserted downstream of P tac . Plasmids pMMB- repA K42A and pMMB- repA D139A have a dominant-negative repA mutant gene, either K42A or D139A, inserted downstream of P tac . b Amount of E. coli DH10B cells retaining the indicated plasmids after 24 h growth in the absence of antibiotics, either with or without IPTG induction. Results were average of three independent experiments, and bars represent mean ± SD (standard deviation). * p

    Article Snippet: E. coli DH10B was purchased from Invitrogen.

    Techniques: Plasmid Preparation, Conjugation Assay, Chick Chorioallantoic Membrane Assay, Dominant Negative Mutation, Mutagenesis, Standard Deviation

    Growth curves of DH10B bacteria expressing wild-type Vpu (top) or the A18H mutant (middle and bottom) as a function of different rimantadine concentrations. The bottom panel is an expansion of the middle panel.

    Journal: PLoS ONE

    Article Title: Bacteria-Based Analysis of HIV-1 Vpu Channel Activity

    doi: 10.1371/journal.pone.0105387

    Figure Lengend Snippet: Growth curves of DH10B bacteria expressing wild-type Vpu (top) or the A18H mutant (middle and bottom) as a function of different rimantadine concentrations. The bottom panel is an expansion of the middle panel.

    Article Snippet: DH10B bacteria cells were purchased from Invitrogen (distributed by Rhenium, Modi'in Israel).

    Techniques: Expressing, Mutagenesis

    Maximal growth rate of DH10B bacteria expressing the Vpu A18H mutant as a function of different rimantadine concentrations. Fitting according to the Monod equation (solid line), to the experimental data (circles).

    Journal: PLoS ONE

    Article Title: Bacteria-Based Analysis of HIV-1 Vpu Channel Activity

    doi: 10.1371/journal.pone.0105387

    Figure Lengend Snippet: Maximal growth rate of DH10B bacteria expressing the Vpu A18H mutant as a function of different rimantadine concentrations. Fitting according to the Monod equation (solid line), to the experimental data (circles).

    Article Snippet: DH10B bacteria cells were purchased from Invitrogen (distributed by Rhenium, Modi'in Israel).

    Techniques: Expressing, Mutagenesis

    Top. Growth curves of DH10B bacteria expressing MBP-Vpu chimera as a function of different HMA concentrations. The bottom panel depicts a toxicity analysis of HMA and rimantadine (Rim.) on bacteria that express MBP without a channel attached to it.

    Journal: PLoS ONE

    Article Title: Bacteria-Based Analysis of HIV-1 Vpu Channel Activity

    doi: 10.1371/journal.pone.0105387

    Figure Lengend Snippet: Top. Growth curves of DH10B bacteria expressing MBP-Vpu chimera as a function of different HMA concentrations. The bottom panel depicts a toxicity analysis of HMA and rimantadine (Rim.) on bacteria that express MBP without a channel attached to it.

    Article Snippet: DH10B bacteria cells were purchased from Invitrogen (distributed by Rhenium, Modi'in Israel).

    Techniques: Expressing

    Growth curves of DH10B bacteria expressing two different Vpu channels: wild-type (a) and the A18H mutant (b) as a function of inducer concentration (IPTG). Both channels are expressed as a chimeric construct fused to the C-terminus of the maltose binding protein (MBP). Panel c, as a control, depicts the same experiment but with MBP without a channel attached to it. Panel d presets a Western blot analysis (using anti-MBP antibody) of chimeric protein expression at different concentrations of the IPTG inducer. An empty plasmid, as a negative control is presented to show expression of native MBP protein in the bacteria (labeled blank).

    Journal: PLoS ONE

    Article Title: Bacteria-Based Analysis of HIV-1 Vpu Channel Activity

    doi: 10.1371/journal.pone.0105387

    Figure Lengend Snippet: Growth curves of DH10B bacteria expressing two different Vpu channels: wild-type (a) and the A18H mutant (b) as a function of inducer concentration (IPTG). Both channels are expressed as a chimeric construct fused to the C-terminus of the maltose binding protein (MBP). Panel c, as a control, depicts the same experiment but with MBP without a channel attached to it. Panel d presets a Western blot analysis (using anti-MBP antibody) of chimeric protein expression at different concentrations of the IPTG inducer. An empty plasmid, as a negative control is presented to show expression of native MBP protein in the bacteria (labeled blank).

    Article Snippet: DH10B bacteria cells were purchased from Invitrogen (distributed by Rhenium, Modi'in Israel).

    Techniques: Expressing, Mutagenesis, Concentration Assay, Construct, Binding Assay, Western Blot, Plasmid Preparation, Negative Control, Labeling