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  • 85
    Bio-Rad dgge denaturing gradient gel electrophoresis analysis
    Dgge Denaturing Gradient Gel Electrophoresis Analysis, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 85/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad dgge analysis dgge
    <t>PCR-DGGE</t> profiles of bacterial cultures after sequential subculturing under different DON concentrations. Passage/cycle numbers and DON concentrations in the utilized media are indicated above each panel. Numbers and positions of absent or weakened DNA bands in correspondence to previous enrichment step are indicated below each panel. Alphabetical letters with arrows indicate the positions of DNA bands that disappeared or (were attenuated) by the next enrichment/subculturing step.
    Dgge Analysis Dgge, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 85/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Illumina Inc dgge analysis
    <t>DGGE</t> analysis of selected samples. Panel A shows representative PCR amplicons from 3 of 20 clinical samples (Subjects 40, 48 and 89) were electrophoresed on a denaturing gradient gel. Bands were excised, sequenced and identified as in the Materials and Methods . Bands are labeled as follows: le = Leptotrichia amnionii ; in = Lactobacillus iners ; ga = Gardnerella vaginalis ; cr = Lactobacillus crispatus ; pr = Prevotella amnii (also named P. amniotica ). Panel B shows a Venn diagram of the organisms identified by <t>Illumina</t> sequencing of the V6 rRNA region and by sequencing DGGE bands amplified from the V3 rRNA region.
    Dgge Analysis, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 92/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega dgge analysis
    <t>DGGE</t> analysis of selected samples. Panel A shows representative PCR amplicons from 3 of 20 clinical samples (Subjects 40, 48 and 89) were electrophoresed on a denaturing gradient gel. Bands were excised, sequenced and identified as in the Materials and Methods . Bands are labeled as follows: le = Leptotrichia amnionii ; in = Lactobacillus iners ; ga = Gardnerella vaginalis ; cr = Lactobacillus crispatus ; pr = Prevotella amnii (also named P. amniotica ). Panel B shows a Venn diagram of the organisms identified by <t>Illumina</t> sequencing of the V6 rRNA region and by sequencing DGGE bands amplified from the V3 rRNA region.
    Dgge Analysis, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 113 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad dgge fingerprint analysis fpquest
    <t>DGGE</t> analysis of selected samples. Panel A shows representative PCR amplicons from 3 of 20 clinical samples (Subjects 40, 48 and 89) were electrophoresed on a denaturing gradient gel. Bands were excised, sequenced and identified as in the Materials and Methods . Bands are labeled as follows: le = Leptotrichia amnionii ; in = Lactobacillus iners ; ga = Gardnerella vaginalis ; cr = Lactobacillus crispatus ; pr = Prevotella amnii (also named P. amniotica ). Panel B shows a Venn diagram of the organisms identified by <t>Illumina</t> sequencing of the V6 rRNA region and by sequencing DGGE bands amplified from the V3 rRNA region.
    Dgge Fingerprint Analysis Fpquest, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Danisco dgge analysis
    <t>DGGE</t> analysis of selected samples. Panel A shows representative PCR amplicons from 3 of 20 clinical samples (Subjects 40, 48 and 89) were electrophoresed on a denaturing gradient gel. Bands were excised, sequenced and identified as in the Materials and Methods . Bands are labeled as follows: le = Leptotrichia amnionii ; in = Lactobacillus iners ; ga = Gardnerella vaginalis ; cr = Lactobacillus crispatus ; pr = Prevotella amnii (also named P. amniotica ). Panel B shows a Venn diagram of the organisms identified by <t>Illumina</t> sequencing of the V6 rRNA region and by sequencing DGGE bands amplified from the V3 rRNA region.
    Dgge Analysis, supplied by Danisco, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    DSMZ dgge analysis
    <t>DGGE</t> analysis of selected samples. Panel A shows representative PCR amplicons from 3 of 20 clinical samples (Subjects 40, 48 and 89) were electrophoresed on a denaturing gradient gel. Bands were excised, sequenced and identified as in the Materials and Methods . Bands are labeled as follows: le = Leptotrichia amnionii ; in = Lactobacillus iners ; ga = Gardnerella vaginalis ; cr = Lactobacillus crispatus ; pr = Prevotella amnii (also named P. amniotica ). Panel B shows a Venn diagram of the organisms identified by <t>Illumina</t> sequencing of the V6 rRNA region and by sequencing DGGE bands amplified from the V3 rRNA region.
    Dgge Analysis, supplied by DSMZ, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Biozym dgge gel analysis
    <t>DGGE</t> analysis of selected samples. Panel A shows representative PCR amplicons from 3 of 20 clinical samples (Subjects 40, 48 and 89) were electrophoresed on a denaturing gradient gel. Bands were excised, sequenced and identified as in the Materials and Methods . Bands are labeled as follows: le = Leptotrichia amnionii ; in = Lactobacillus iners ; ga = Gardnerella vaginalis ; cr = Lactobacillus crispatus ; pr = Prevotella amnii (also named P. amniotica ). Panel B shows a Venn diagram of the organisms identified by <t>Illumina</t> sequencing of the V6 rRNA region and by sequencing DGGE bands amplified from the V3 rRNA region.
    Dgge Gel Analysis, supplied by Biozym, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad dgge analysis pcr amplicons
    <t>DGGE</t> analysis of selected samples. Panel A shows representative PCR amplicons from 3 of 20 clinical samples (Subjects 40, 48 and 89) were electrophoresed on a denaturing gradient gel. Bands were excised, sequenced and identified as in the Materials and Methods . Bands are labeled as follows: le = Leptotrichia amnionii ; in = Lactobacillus iners ; ga = Gardnerella vaginalis ; cr = Lactobacillus crispatus ; pr = Prevotella amnii (also named P. amniotica ). Panel B shows a Venn diagram of the organisms identified by <t>Illumina</t> sequencing of the V6 rRNA region and by sequencing DGGE bands amplified from the V3 rRNA region.
    Dgge Analysis Pcr Amplicons, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 85/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad dcode mutation analysis system
    <t>DGGE</t> analysis of selected samples. Panel A shows representative PCR amplicons from 3 of 20 clinical samples (Subjects 40, 48 and 89) were electrophoresed on a denaturing gradient gel. Bands were excised, sequenced and identified as in the Materials and Methods . Bands are labeled as follows: le = Leptotrichia amnionii ; in = Lactobacillus iners ; ga = Gardnerella vaginalis ; cr = Lactobacillus crispatus ; pr = Prevotella amnii (also named P. amniotica ). Panel B shows a Venn diagram of the organisms identified by <t>Illumina</t> sequencing of the V6 rRNA region and by sequencing DGGE bands amplified from the V3 rRNA region.
    Dcode Mutation Analysis System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad gradient gel electrophoresis dgge analysis
    Denaturing gradient gel electrophoresis analysis of <t>thaumarchaeal</t> ( a , c ) and bacterial ( b ) amoA genes during DNA-SIP microcosms of HZ soil incubated with 13 C-CO 2 or 12 C-CO 2 in the presence or absence of DCD. Thaumrchaeal amoA gene PCR products of twenty fractions of genomic DNA from individual centrifuge tube were subjected to <t>DGGE</t> for changes of different thaumarchaeal communitys for 15 or 30 days ( b ). Eight arrows marked the bands in heavy fraction around a buoyant density of 1.73 g ml −1 excised for sequencing.
    Gradient Gel Electrophoresis Dgge Analysis, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen dgge analysis a qiaamp dna stool mini kit
    Denaturing gradient gel electrophoresis analysis of <t>thaumarchaeal</t> ( a , c ) and bacterial ( b ) amoA genes during DNA-SIP microcosms of HZ soil incubated with 13 C-CO 2 or 12 C-CO 2 in the presence or absence of DCD. Thaumrchaeal amoA gene PCR products of twenty fractions of genomic DNA from individual centrifuge tube were subjected to <t>DGGE</t> for changes of different thaumarchaeal communitys for 15 or 30 days ( b ). Eight arrows marked the bands in heavy fraction around a buoyant density of 1.73 g ml −1 excised for sequencing.
    Dgge Analysis A Qiaamp Dna Stool Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    PCR-DGGE profiles of bacterial cultures after sequential subculturing under different DON concentrations. Passage/cycle numbers and DON concentrations in the utilized media are indicated above each panel. Numbers and positions of absent or weakened DNA bands in correspondence to previous enrichment step are indicated below each panel. Alphabetical letters with arrows indicate the positions of DNA bands that disappeared or (were attenuated) by the next enrichment/subculturing step.

    Journal: Toxins

    Article Title: Aerobic De-Epoxydation of Trichothecene Mycotoxins by a Soil Bacterial Consortium Isolated Using In Situ Soil Enrichment

    doi: 10.3390/toxins8100277

    Figure Lengend Snippet: PCR-DGGE profiles of bacterial cultures after sequential subculturing under different DON concentrations. Passage/cycle numbers and DON concentrations in the utilized media are indicated above each panel. Numbers and positions of absent or weakened DNA bands in correspondence to previous enrichment step are indicated below each panel. Alphabetical letters with arrows indicate the positions of DNA bands that disappeared or (were attenuated) by the next enrichment/subculturing step.

    Article Snippet: The DGGE analysis of PCR amplicons was performed using Bio-Rad DCode Universal Mutation Detection System (Bio-Rad, Hercules, CA, USA) as described previously [ ].

    Techniques: Polymerase Chain Reaction, Denaturing Gradient Gel Electrophoresis, Subculturing Assay

    DGGE profiles revealed microbial diversity in the cecum content and fecal pellets of mice exposed for 8 weeks to Cd and Pb salts via their drinking water. The figure shows DGGE gels of the V5-V6 hypervariable 16S rDNA region, illustrating the microbiota’s composition in the cecum and the feces of 4 mice treated (or not) with 20 mg L -1 (ppm) of Cd or 100 mg L -1 (ppm) of Pb.

    Journal: BMC Pharmacology & Toxicology

    Article Title: Ecotoxicology inside the gut: impact of heavy metals on the mouse microbiome

    doi: 10.1186/2050-6511-14-62

    Figure Lengend Snippet: DGGE profiles revealed microbial diversity in the cecum content and fecal pellets of mice exposed for 8 weeks to Cd and Pb salts via their drinking water. The figure shows DGGE gels of the V5-V6 hypervariable 16S rDNA region, illustrating the microbiota’s composition in the cecum and the feces of 4 mice treated (or not) with 20 mg L -1 (ppm) of Cd or 100 mg L -1 (ppm) of Pb.

    Article Snippet: The resulting 16S rRNA amplicons were analyzed by DGGE fingerprinting analysis (the D-Code System from Bio-Rad, Nazareth, Belgium) using 35% to 70% denaturing gels, as previously described [ ].

    Techniques: Denaturing Gradient Gel Electrophoresis, Mouse Assay

    DGGE analysis of selected samples. Panel A shows representative PCR amplicons from 3 of 20 clinical samples (Subjects 40, 48 and 89) were electrophoresed on a denaturing gradient gel. Bands were excised, sequenced and identified as in the Materials and Methods . Bands are labeled as follows: le = Leptotrichia amnionii ; in = Lactobacillus iners ; ga = Gardnerella vaginalis ; cr = Lactobacillus crispatus ; pr = Prevotella amnii (also named P. amniotica ). Panel B shows a Venn diagram of the organisms identified by Illumina sequencing of the V6 rRNA region and by sequencing DGGE bands amplified from the V3 rRNA region.

    Journal: PLoS ONE

    Article Title: Microbiome Profiling by Illumina Sequencing of Combinatorial Sequence-Tagged PCR Products

    doi: 10.1371/journal.pone.0015406

    Figure Lengend Snippet: DGGE analysis of selected samples. Panel A shows representative PCR amplicons from 3 of 20 clinical samples (Subjects 40, 48 and 89) were electrophoresed on a denaturing gradient gel. Bands were excised, sequenced and identified as in the Materials and Methods . Bands are labeled as follows: le = Leptotrichia amnionii ; in = Lactobacillus iners ; ga = Gardnerella vaginalis ; cr = Lactobacillus crispatus ; pr = Prevotella amnii (also named P. amniotica ). Panel B shows a Venn diagram of the organisms identified by Illumina sequencing of the V6 rRNA region and by sequencing DGGE bands amplified from the V3 rRNA region.

    Article Snippet: In two cases, one shown in -lane 89, a distinct band was excised and sequenced that had an Illumina abundance of between 2–3%. shows that a total of 8 organisms were detected through DGGE analysis, compared to 59 organisms detected through Illumina analysis in the same 20 samples, and that the organisms identified by DGGE analysis were a strict subset of those identified by Illumina sequencing.

    Techniques: Denaturing Gradient Gel Electrophoresis, Polymerase Chain Reaction, Labeling, Sequencing, Amplification

    Denaturing gradient gel electrophoresis analysis of thaumarchaeal ( a , c ) and bacterial ( b ) amoA genes during DNA-SIP microcosms of HZ soil incubated with 13 C-CO 2 or 12 C-CO 2 in the presence or absence of DCD. Thaumrchaeal amoA gene PCR products of twenty fractions of genomic DNA from individual centrifuge tube were subjected to DGGE for changes of different thaumarchaeal communitys for 15 or 30 days ( b ). Eight arrows marked the bands in heavy fraction around a buoyant density of 1.73 g ml −1 excised for sequencing.

    Journal: The ISME Journal

    Article Title: Ammonia-oxidizing archaea have more important role than ammonia-oxidizing bacteria in ammonia oxidation of strongly acidic soils

    doi: 10.1038/ismej.2011.168

    Figure Lengend Snippet: Denaturing gradient gel electrophoresis analysis of thaumarchaeal ( a , c ) and bacterial ( b ) amoA genes during DNA-SIP microcosms of HZ soil incubated with 13 C-CO 2 or 12 C-CO 2 in the presence or absence of DCD. Thaumrchaeal amoA gene PCR products of twenty fractions of genomic DNA from individual centrifuge tube were subjected to DGGE for changes of different thaumarchaeal communitys for 15 or 30 days ( b ). Eight arrows marked the bands in heavy fraction around a buoyant density of 1.73 g ml −1 excised for sequencing.

    Article Snippet: Denaturing gradient gel electrophoresis (DGGE) analysis of thaumarchaeal and bacterial ammonia oxidizers was performed using a DCode Universal Mutation Detection System (Bio-Rad Laboratories).

    Techniques: Denaturing Gradient Gel Electrophoresis, Incubation, Polymerase Chain Reaction, Sequencing

    Phylogenetic analysis of archaeal 16S rRNA genes ( a ) retrieved from 13 C-CO 2 DNA-SIP heavy fractions (HF) and light fractions (LF) of HZ soil microcosms incubated for 30 days, and thaumarchaeal amoA genes ( b ) derived from eight DGGE bands in the heavy fractions around a buoyant density of 1.73 g ml −1 from the HZ soil incubated for 30 days. The sequences for the HF and LF were highlighted in red and blue, respectively. HF-Archaea-clone-3-(19) indicates that 19 clones showed above 98% similarities to the clone-3. Bootstrap values ( > 50%) are indicated at branch points. The scale bar represents 2% and 5% nucleic acid sequence divergence for archaeal 16S rRNA genes and thaumarchael amoA genes, respectively. The letters M and S represent Marine Lineage and Soil and Sediment Lineage, respectively.

    Journal: The ISME Journal

    Article Title: Ammonia-oxidizing archaea have more important role than ammonia-oxidizing bacteria in ammonia oxidation of strongly acidic soils

    doi: 10.1038/ismej.2011.168

    Figure Lengend Snippet: Phylogenetic analysis of archaeal 16S rRNA genes ( a ) retrieved from 13 C-CO 2 DNA-SIP heavy fractions (HF) and light fractions (LF) of HZ soil microcosms incubated for 30 days, and thaumarchaeal amoA genes ( b ) derived from eight DGGE bands in the heavy fractions around a buoyant density of 1.73 g ml −1 from the HZ soil incubated for 30 days. The sequences for the HF and LF were highlighted in red and blue, respectively. HF-Archaea-clone-3-(19) indicates that 19 clones showed above 98% similarities to the clone-3. Bootstrap values ( > 50%) are indicated at branch points. The scale bar represents 2% and 5% nucleic acid sequence divergence for archaeal 16S rRNA genes and thaumarchael amoA genes, respectively. The letters M and S represent Marine Lineage and Soil and Sediment Lineage, respectively.

    Article Snippet: Denaturing gradient gel electrophoresis (DGGE) analysis of thaumarchaeal and bacterial ammonia oxidizers was performed using a DCode Universal Mutation Detection System (Bio-Rad Laboratories).

    Techniques: Incubation, Derivative Assay, Denaturing Gradient Gel Electrophoresis, Clone Assay, Sequencing