dephosphorylation Search Results


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  • 95
    New England Biolabs quick dephosphorylation kit
    Quick Dephosphorylation Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher t4 polynucleotide kinase
    T4 Polynucleotide Kinase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 9937 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Cell Signaling Technology Inc dephosphorylation
    Dephosphorylation, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Millipore casein
    Effects of ABA on the protein phosphorylation activities of SnRK2s and casein kinases in Arabidopsis . Gels contain <t>α-casein</t> ( left panel ) and histone ( right panel ) as substrates. Total protein extracts from control and ABA-treated seedlings were
    Casein, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 2804 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher dephosphorylation
    Effects of ABA on the protein phosphorylation activities of SnRK2s and casein kinases in Arabidopsis . Gels contain <t>α-casein</t> ( left panel ) and histone ( right panel ) as substrates. Total protein extracts from control and ABA-treated seedlings were
    Dephosphorylation, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 161 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Millipore bovine casein
    Effects of ABA on the protein phosphorylation activities of SnRK2s and casein kinases in Arabidopsis . Gels contain <t>α-casein</t> ( left panel ) and histone ( right panel ) as substrates. Total protein extracts from control and ABA-treated seedlings were
    Bovine Casein, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 87 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Thermo Fisher dephosphorylation buffer
    RNA-binding activity of p45, p39, and p26 depends on their phosphorylation status. Two micrograms of liver nuclear extract from untreated or CTL-injected mice was treated with 0.5 and 1.0 U of CIAP (alk. phos.) prior to addition of 40 fmol of 32 P-labeled in vitro transcript RNA.B. The <t>dephosphorylation</t> reaction was performed in 20 μl of 1× reaction buffer for 30 min at 37°C. The binding reaction was performed and analyzed as described in Materials and Methods.
    Dephosphorylation Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Epitope Biotech tau dephosphorylation
    RNA-binding activity of p45, p39, and p26 depends on their phosphorylation status. Two micrograms of liver nuclear extract from untreated or CTL-injected mice was treated with 0.5 and 1.0 U of CIAP (alk. phos.) prior to addition of 40 fmol of 32 P-labeled in vitro transcript RNA.B. The <t>dephosphorylation</t> reaction was performed in 20 μl of 1× reaction buffer for 30 min at 37°C. The binding reaction was performed and analyzed as described in Materials and Methods.
    Tau Dephosphorylation, supplied by Epitope Biotech, used in various techniques. Bioz Stars score: 91/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    GE Healthcare alkaline phosphatase dephosphorylates ecf substrate
    RNA-binding activity of p45, p39, and p26 depends on their phosphorylation status. Two micrograms of liver nuclear extract from untreated or CTL-injected mice was treated with 0.5 and 1.0 U of CIAP (alk. phos.) prior to addition of 40 fmol of 32 P-labeled in vitro transcript RNA.B. The <t>dephosphorylation</t> reaction was performed in 20 μl of 1× reaction buffer for 30 min at 37°C. The binding reaction was performed and analyzed as described in Materials and Methods.
    Alkaline Phosphatase Dephosphorylates Ecf Substrate, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 96/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    New England Biolabs lambda protein phosphatase assay dephosphorylation
    RNA-binding activity of p45, p39, and p26 depends on their phosphorylation status. Two micrograms of liver nuclear extract from untreated or CTL-injected mice was treated with 0.5 and 1.0 U of CIAP (alk. phos.) prior to addition of 40 fmol of 32 P-labeled in vitro transcript RNA.B. The <t>dephosphorylation</t> reaction was performed in 20 μl of 1× reaction buffer for 30 min at 37°C. The binding reaction was performed and analyzed as described in Materials and Methods.
    Lambda Protein Phosphatase Assay Dephosphorylation, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs t4 polynucleotide kinase
    RNA-binding activity of p45, p39, and p26 depends on their phosphorylation status. Two micrograms of liver nuclear extract from untreated or CTL-injected mice was treated with 0.5 and 1.0 U of CIAP (alk. phos.) prior to addition of 40 fmol of 32 P-labeled in vitro transcript RNA.B. The <t>dephosphorylation</t> reaction was performed in 20 μl of 1× reaction buffer for 30 min at 37°C. The binding reaction was performed and analyzed as described in Materials and Methods.
    T4 Polynucleotide Kinase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 29387 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Merck KGaA bovine dephosphorylated mbp
    RNA-binding activity of p45, p39, and p26 depends on their phosphorylation status. Two micrograms of liver nuclear extract from untreated or CTL-injected mice was treated with 0.5 and 1.0 U of CIAP (alk. phos.) prior to addition of 40 fmol of 32 P-labeled in vitro transcript RNA.B. The <t>dephosphorylation</t> reaction was performed in 20 μl of 1× reaction buffer for 30 min at 37°C. The binding reaction was performed and analyzed as described in Materials and Methods.
    Bovine Dephosphorylated Mbp, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Valiant plasmid dephosphorylation
    RNA-binding activity of p45, p39, and p26 depends on their phosphorylation status. Two micrograms of liver nuclear extract from untreated or CTL-injected mice was treated with 0.5 and 1.0 U of CIAP (alk. phos.) prior to addition of 40 fmol of 32 P-labeled in vitro transcript RNA.B. The <t>dephosphorylation</t> reaction was performed in 20 μl of 1× reaction buffer for 30 min at 37°C. The binding reaction was performed and analyzed as described in Materials and Methods.
    Plasmid Dephosphorylation, supplied by Valiant, used in various techniques. Bioz Stars score: 92/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Merck KGaA mouse anti active dephosphorylated β catenin
    RNA-binding activity of p45, p39, and p26 depends on their phosphorylation status. Two micrograms of liver nuclear extract from untreated or CTL-injected mice was treated with 0.5 and 1.0 U of CIAP (alk. phos.) prior to addition of 40 fmol of 32 P-labeled in vitro transcript RNA.B. The <t>dephosphorylation</t> reaction was performed in 20 μl of 1× reaction buffer for 30 min at 37°C. The binding reaction was performed and analyzed as described in Materials and Methods.
    Mouse Anti Active Dephosphorylated β Catenin, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher dephosphorylated expression vector pcdna 3
    RNA-binding activity of p45, p39, and p26 depends on their phosphorylation status. Two micrograms of liver nuclear extract from untreated or CTL-injected mice was treated with 0.5 and 1.0 U of CIAP (alk. phos.) prior to addition of 40 fmol of 32 P-labeled in vitro transcript RNA.B. The <t>dephosphorylation</t> reaction was performed in 20 μl of 1× reaction buffer for 30 min at 37°C. The binding reaction was performed and analyzed as described in Materials and Methods.
    Dephosphorylated Expression Vector Pcdna 3, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher dephosphorylated pcdna3 vector
    RNA-binding activity of p45, p39, and p26 depends on their phosphorylation status. Two micrograms of liver nuclear extract from untreated or CTL-injected mice was treated with 0.5 and 1.0 U of CIAP (alk. phos.) prior to addition of 40 fmol of 32 P-labeled in vitro transcript RNA.B. The <t>dephosphorylation</t> reaction was performed in 20 μl of 1× reaction buffer for 30 min at 37°C. The binding reaction was performed and analyzed as described in Materials and Methods.
    Dephosphorylated Pcdna3 Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Promega dephosphorylation buffer
    HPLC-MS traces of the (a) mixture of nucleosides used as a standard and (b) digestion results of transcript 5 . Digestion of 1–2 nmol of transcript was carried out using S1 nuclease for 2 h at 37 °C and followed by <t>dephosphorylation</t> with alkaline phosphatase for 2 h at 37 °C. The ribonucleoside mixture obtained was analyzed by reverse-phase analytical HPLC, using a mobile phase of 0–6% acetonitrile (0.1% formic acid) in water (0.1% formic acid) over 12 min; flow rate 1 mL/min.
    Dephosphorylation Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher dephosphorylation inhibitors
    HPLC-MS traces of the (a) mixture of nucleosides used as a standard and (b) digestion results of transcript 5 . Digestion of 1–2 nmol of transcript was carried out using S1 nuclease for 2 h at 37 °C and followed by <t>dephosphorylation</t> with alkaline phosphatase for 2 h at 37 °C. The ribonucleoside mixture obtained was analyzed by reverse-phase analytical HPLC, using a mobile phase of 0–6% acetonitrile (0.1% formic acid) in water (0.1% formic acid) over 12 min; flow rate 1 mL/min.
    Dephosphorylation Inhibitors, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore dephosphorylation
    HPLC-MS traces of the (a) mixture of nucleosides used as a standard and (b) digestion results of transcript 5 . Digestion of 1–2 nmol of transcript was carried out using S1 nuclease for 2 h at 37 °C and followed by <t>dephosphorylation</t> with alkaline phosphatase for 2 h at 37 °C. The ribonucleoside mixture obtained was analyzed by reverse-phase analytical HPLC, using a mobile phase of 0–6% acetonitrile (0.1% formic acid) in water (0.1% formic acid) over 12 min; flow rate 1 mL/min.
    Dephosphorylation, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 82 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Syntaxin dephosphorylation
    HPLC-MS traces of the (a) mixture of nucleosides used as a standard and (b) digestion results of transcript 5 . Digestion of 1–2 nmol of transcript was carried out using S1 nuclease for 2 h at 37 °C and followed by <t>dephosphorylation</t> with alkaline phosphatase for 2 h at 37 °C. The ribonucleoside mixture obtained was analyzed by reverse-phase analytical HPLC, using a mobile phase of 0–6% acetonitrile (0.1% formic acid) in water (0.1% formic acid) over 12 min; flow rate 1 mL/min.
    Dephosphorylation, supplied by Syntaxin, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Glycoform major dephosphorylated o los glycoform
    Negative-ion MALDI time-of-flight mass spectra of <t>dephosphorylated</t> O <t>-LOS</t> from H. influenzae stains R2866 (A) and R3392 (B). Note the differences between the two panels in peaks C (representing a <t>glycoform</t> with three hexoses and four heptoses) and D (four
    Major Dephosphorylated O Los Glycoform, supplied by Glycoform, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher xmni ecorv digested dephosphorylated pentr1a
    Negative-ion MALDI time-of-flight mass spectra of <t>dephosphorylated</t> O <t>-LOS</t> from H. influenzae stains R2866 (A) and R3392 (B). Note the differences between the two panels in peaks C (representing a <t>glycoform</t> with three hexoses and four heptoses) and D (four
    Xmni Ecorv Digested Dephosphorylated Pentr1a, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Effects of ABA on the protein phosphorylation activities of SnRK2s and casein kinases in Arabidopsis . Gels contain α-casein ( left panel ) and histone ( right panel ) as substrates. Total protein extracts from control and ABA-treated seedlings were

    Journal: Methods in molecular biology (Clifton, N.J.)

    Article Title: Assessing Kinase Activity in Plants with In-Gel Kinase Assays

    doi: 10.1007/978-1-4939-3115-6_15

    Figure Lengend Snippet: Effects of ABA on the protein phosphorylation activities of SnRK2s and casein kinases in Arabidopsis . Gels contain α-casein ( left panel ) and histone ( right panel ) as substrates. Total protein extracts from control and ABA-treated seedlings were

    Article Snippet: Store as 1-mL aliquots at −20 °C. α-Casein solution 10 mg/mL: Dissolve 100 mg dephosphorylated α-casein (Sigma-Aldrich, St Louis, MO, USA, C-8032) in 10 mL distilled water.

    Techniques:

    Pairwise normalization developed for label-free quantitative phosphoproteomics. ( a ) HeLa cells with different treatments were subjected to cell lysis, spiking  α -casein standard, and tryptic digestion. Peptides with and without TiO 2  phosphopeptide enrichment were analyzed by LC-MS/MS. Peptides were identified by Mascot database search, followed by phosphorylation site validation by phosphoRS. Phosphopeptide identification was supplemented by SimSpectraST spectral library search. Following label-free quantification, peptide abundance was normalized with different methods, including the pairwise normalization for TiO 2  data developed in this study. ( b ) The principle of the pairwise normalization method. Fifty-two phosphopeptides were quantified in both the non-enriched digests and TiO 2 -enriched samples (i.e. 52 digest-TiO 2  pairs). Abundance profiles of two hypothetical phosphopeptides are illustrated as examples. An abundance ratio was calculated by pairwise comparison (digest/TiO 2 ) for each phosphopeptide. Eleven pairs were excluded as outliers (see the criteria in  Supplementary Fig. 3 ). The median of normalized abundance ratios was then calculated for the remaining 41 pairs and used as a pairwise normalization factor for the TiO 2  data. The TiO 2  data were pre-normalized with the global centering method, whereas the digest data were normalized with the global centering or quantile centering method (i.e. global pairwise and quantile pairwise, respectively).

    Journal: Scientific Reports

    Article Title: Label-free quantitative phosphoproteomics with novel pairwise abundance normalization reveals synergistic RAS and CIP2A signaling

    doi: 10.1038/srep13099

    Figure Lengend Snippet: Pairwise normalization developed for label-free quantitative phosphoproteomics. ( a ) HeLa cells with different treatments were subjected to cell lysis, spiking α -casein standard, and tryptic digestion. Peptides with and without TiO 2 phosphopeptide enrichment were analyzed by LC-MS/MS. Peptides were identified by Mascot database search, followed by phosphorylation site validation by phosphoRS. Phosphopeptide identification was supplemented by SimSpectraST spectral library search. Following label-free quantification, peptide abundance was normalized with different methods, including the pairwise normalization for TiO 2 data developed in this study. ( b ) The principle of the pairwise normalization method. Fifty-two phosphopeptides were quantified in both the non-enriched digests and TiO 2 -enriched samples (i.e. 52 digest-TiO 2 pairs). Abundance profiles of two hypothetical phosphopeptides are illustrated as examples. An abundance ratio was calculated by pairwise comparison (digest/TiO 2 ) for each phosphopeptide. Eleven pairs were excluded as outliers (see the criteria in Supplementary Fig. 3 ). The median of normalized abundance ratios was then calculated for the remaining 41 pairs and used as a pairwise normalization factor for the TiO 2 data. The TiO 2 data were pre-normalized with the global centering method, whereas the digest data were normalized with the global centering or quantile centering method (i.e. global pairwise and quantile pairwise, respectively).

    Article Snippet: Digestion and TiO2 phosphopeptide enrichment The lysates (1 mg protein) were diluted to 200 μl with a buffer containing 8 M urea and 50 mM Tris-HCl pH 8.5, after spiking in 10 μl of 1 μg/μl bovine α-casein (Sigma-Aldrich).

    Techniques: Lysis, Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry

    RNA-binding activity of p45, p39, and p26 depends on their phosphorylation status. Two micrograms of liver nuclear extract from untreated or CTL-injected mice was treated with 0.5 and 1.0 U of CIAP (alk. phos.) prior to addition of 40 fmol of 32 P-labeled in vitro transcript RNA.B. The dephosphorylation reaction was performed in 20 μl of 1× reaction buffer for 30 min at 37°C. The binding reaction was performed and analyzed as described in Materials and Methods.

    Journal: Journal of Virology

    Article Title: La Autoantigen Specifically Recognizes a Predicted Stem-Loop in Hepatitis B Virus RNA †

    doi:

    Figure Lengend Snippet: RNA-binding activity of p45, p39, and p26 depends on their phosphorylation status. Two micrograms of liver nuclear extract from untreated or CTL-injected mice was treated with 0.5 and 1.0 U of CIAP (alk. phos.) prior to addition of 40 fmol of 32 P-labeled in vitro transcript RNA.B. The dephosphorylation reaction was performed in 20 μl of 1× reaction buffer for 30 min at 37°C. The binding reaction was performed and analyzed as described in Materials and Methods.

    Article Snippet: In the dephosphorylation reaction, 5 to 10 μg of nuclear extract was treated with 0.5 to 3 U of calf intestine alkaline phosphatase (CIAP) (Ambion, Austin, Tex.) for 30 min at 37°C in 20 μl of 1× dephosphorylation buffer (Ambion).

    Techniques: RNA Binding Assay, Activity Assay, CTL Assay, Injection, Mouse Assay, Labeling, In Vitro, De-Phosphorylation Assay, Binding Assay

    HPLC-MS traces of the (a) mixture of nucleosides used as a standard and (b) digestion results of transcript 5 . Digestion of 1–2 nmol of transcript was carried out using S1 nuclease for 2 h at 37 °C and followed by dephosphorylation with alkaline phosphatase for 2 h at 37 °C. The ribonucleoside mixture obtained was analyzed by reverse-phase analytical HPLC, using a mobile phase of 0–6% acetonitrile (0.1% formic acid) in water (0.1% formic acid) over 12 min; flow rate 1 mL/min.

    Journal: Journal of the American Chemical Society

    Article Title: Isomorphic Emissive GTP Surrogate Facilitates Initiation and Elongation of in Vitro Transcription Reactions

    doi: 10.1021/ja5039227

    Figure Lengend Snippet: HPLC-MS traces of the (a) mixture of nucleosides used as a standard and (b) digestion results of transcript 5 . Digestion of 1–2 nmol of transcript was carried out using S1 nuclease for 2 h at 37 °C and followed by dephosphorylation with alkaline phosphatase for 2 h at 37 °C. The ribonucleoside mixture obtained was analyzed by reverse-phase analytical HPLC, using a mobile phase of 0–6% acetonitrile (0.1% formic acid) in water (0.1% formic acid) over 12 min; flow rate 1 mL/min.

    Article Snippet: The reaction was further treated with alkaline phosphatase and dephosphorylation buffer (Promega) for 2 h at 37 °C.

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry, De-Phosphorylation Assay, Flow Cytometry

    Negative-ion MALDI time-of-flight mass spectra of dephosphorylated O -LOS from H. influenzae stains R2866 (A) and R3392 (B). Note the differences between the two panels in peaks C (representing a glycoform with three hexoses and four heptoses) and D (four

    Journal: Infection and Immunity

    Article Title: Role of lgtC in Resistance of Nontypeable Haemophilus influenzae Strain R2866 to Human Serum ▿

    doi: 10.1128/IAI.00722-06

    Figure Lengend Snippet: Negative-ion MALDI time-of-flight mass spectra of dephosphorylated O -LOS from H. influenzae stains R2866 (A) and R3392 (B). Note the differences between the two panels in peaks C (representing a glycoform with three hexoses and four heptoses) and D (four

    Article Snippet: The major dephosphorylated O-LOS glycoform (peak C in Fig. ) in strain R3392 had an abundant deprotonated molecular ion, [M-H]− at m/z 2,267.3, consistent with the structure lipid A′-KDO-Hep4 -Hex3 , where lipid A′ refers to the di-N-acyl-dephosphorylated form.

    Techniques: