depc-treated water Search Results


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  • 95
    Qiagen rnase free depc treated water
    Rnase Free Depc Treated Water, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnase free depc treated water/product/Qiagen
    Average 95 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    rnase free depc treated water - by Bioz Stars, 2020-02
    95/100 stars
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    95
    Millipore diethyl pyrocarbonate
    Inhibition of the malate-aspartate shuttle restores the ability of lactate to activate NF-κB in oxidative Cal27 tumor cells . (A) The basal, maximal and OXPHOS-dependent oxygen consumption rates of HeLa and Cal27 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (B) Representative western blots showing Nox1, Nox2, Nox4 expression in Cal27 cells. (C) Cal27 cells were treated for the indicated amounts of time with vehicle (control, C), 1 mM of diethyl <t>pyrocarbonate</t> (DEPC, D), 10 mM Lactate (L), the combination of DEPC and lactate (DL), or TNFα (20 ng/ml), after which p-Ser536-p65 and total p65 were detected in cell lysates. Representative western blots are shown. (D) Cal27 cells were treated as in (C) except that NF-κB activity was determined using a dual luciferase reporter assay 1, 2, and 3 h after treatment ( n = 4). (E) The basal, maximal and OXPHOS-dependent oxygen consumption rate of SiHa and MCF7 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (F) Representative western blots showing Nox1, Nox2, Nox4 expression in MCF7 cells. (G) MCF7 cells were treated for 3 h with vehicle (control, C), 1 mM of DEPC (D), 10 mM of L-Lactate (L) or combination (DL), after which p-Ser536-p65, total p65 and β-actin were detected in cell lysates. A 1-h treatment with TNFα was used as positive control. Representative western blots are shown. (H) Same as in (G) except that NF-κB activity was determined using a dual luciferase reporter assay ( n = 4). All data represent means ± SEM. * P
    Diethyl Pyrocarbonate, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 548 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/diethyl pyrocarbonate/product/Millipore
    Average 95 stars, based on 548 article reviews
    Price from $9.99 to $1999.99
    diethyl pyrocarbonate - by Bioz Stars, 2020-02
    95/100 stars
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    90
    Thermo Fisher ultrapure depc treated water
    Inhibition of the malate-aspartate shuttle restores the ability of lactate to activate NF-κB in oxidative Cal27 tumor cells . (A) The basal, maximal and OXPHOS-dependent oxygen consumption rates of HeLa and Cal27 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (B) Representative western blots showing Nox1, Nox2, Nox4 expression in Cal27 cells. (C) Cal27 cells were treated for the indicated amounts of time with vehicle (control, C), 1 mM of diethyl <t>pyrocarbonate</t> (DEPC, D), 10 mM Lactate (L), the combination of DEPC and lactate (DL), or TNFα (20 ng/ml), after which p-Ser536-p65 and total p65 were detected in cell lysates. Representative western blots are shown. (D) Cal27 cells were treated as in (C) except that NF-κB activity was determined using a dual luciferase reporter assay 1, 2, and 3 h after treatment ( n = 4). (E) The basal, maximal and OXPHOS-dependent oxygen consumption rate of SiHa and MCF7 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (F) Representative western blots showing Nox1, Nox2, Nox4 expression in MCF7 cells. (G) MCF7 cells were treated for 3 h with vehicle (control, C), 1 mM of DEPC (D), 10 mM of L-Lactate (L) or combination (DL), after which p-Ser536-p65, total p65 and β-actin were detected in cell lysates. A 1-h treatment with TNFα was used as positive control. Representative western blots are shown. (H) Same as in (G) except that NF-κB activity was determined using a dual luciferase reporter assay ( n = 4). All data represent means ± SEM. * P
    Ultrapure Depc Treated Water, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 135 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ultrapure depc treated water/product/Thermo Fisher
    Average 90 stars, based on 135 article reviews
    Price from $9.99 to $1999.99
    ultrapure depc treated water - by Bioz Stars, 2020-02
    90/100 stars
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    95
    Millipore depc treated water
    Inhibition of the malate-aspartate shuttle restores the ability of lactate to activate NF-κB in oxidative Cal27 tumor cells . (A) The basal, maximal and OXPHOS-dependent oxygen consumption rates of HeLa and Cal27 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (B) Representative western blots showing Nox1, Nox2, Nox4 expression in Cal27 cells. (C) Cal27 cells were treated for the indicated amounts of time with vehicle (control, C), 1 mM of diethyl <t>pyrocarbonate</t> (DEPC, D), 10 mM Lactate (L), the combination of DEPC and lactate (DL), or TNFα (20 ng/ml), after which p-Ser536-p65 and total p65 were detected in cell lysates. Representative western blots are shown. (D) Cal27 cells were treated as in (C) except that NF-κB activity was determined using a dual luciferase reporter assay 1, 2, and 3 h after treatment ( n = 4). (E) The basal, maximal and OXPHOS-dependent oxygen consumption rate of SiHa and MCF7 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (F) Representative western blots showing Nox1, Nox2, Nox4 expression in MCF7 cells. (G) MCF7 cells were treated for 3 h with vehicle (control, C), 1 mM of DEPC (D), 10 mM of L-Lactate (L) or combination (DL), after which p-Ser536-p65, total p65 and β-actin were detected in cell lysates. A 1-h treatment with TNFα was used as positive control. Representative western blots are shown. (H) Same as in (G) except that NF-κB activity was determined using a dual luciferase reporter assay ( n = 4). All data represent means ± SEM. * P
    Depc Treated Water, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 357 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/depc treated water/product/Millipore
    Average 95 stars, based on 357 article reviews
    Price from $9.99 to $1999.99
    depc treated water - by Bioz Stars, 2020-02
    95/100 stars
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    94
    Sangon Biotech depc treated water
    Inhibition of the malate-aspartate shuttle restores the ability of lactate to activate NF-κB in oxidative Cal27 tumor cells . (A) The basal, maximal and OXPHOS-dependent oxygen consumption rates of HeLa and Cal27 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (B) Representative western blots showing Nox1, Nox2, Nox4 expression in Cal27 cells. (C) Cal27 cells were treated for the indicated amounts of time with vehicle (control, C), 1 mM of diethyl <t>pyrocarbonate</t> (DEPC, D), 10 mM Lactate (L), the combination of DEPC and lactate (DL), or TNFα (20 ng/ml), after which p-Ser536-p65 and total p65 were detected in cell lysates. Representative western blots are shown. (D) Cal27 cells were treated as in (C) except that NF-κB activity was determined using a dual luciferase reporter assay 1, 2, and 3 h after treatment ( n = 4). (E) The basal, maximal and OXPHOS-dependent oxygen consumption rate of SiHa and MCF7 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (F) Representative western blots showing Nox1, Nox2, Nox4 expression in MCF7 cells. (G) MCF7 cells were treated for 3 h with vehicle (control, C), 1 mM of DEPC (D), 10 mM of L-Lactate (L) or combination (DL), after which p-Ser536-p65, total p65 and β-actin were detected in cell lysates. A 1-h treatment with TNFα was used as positive control. Representative western blots are shown. (H) Same as in (G) except that NF-κB activity was determined using a dual luciferase reporter assay ( n = 4). All data represent means ± SEM. * P
    Depc Treated Water, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 94/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/depc treated water/product/Sangon Biotech
    Average 94 stars, based on 40 article reviews
    Price from $9.99 to $1999.99
    depc treated water - by Bioz Stars, 2020-02
    94/100 stars
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    90
    Bioneer Corporation depc treated water
    Inhibition of the malate-aspartate shuttle restores the ability of lactate to activate NF-κB in oxidative Cal27 tumor cells . (A) The basal, maximal and OXPHOS-dependent oxygen consumption rates of HeLa and Cal27 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (B) Representative western blots showing Nox1, Nox2, Nox4 expression in Cal27 cells. (C) Cal27 cells were treated for the indicated amounts of time with vehicle (control, C), 1 mM of diethyl <t>pyrocarbonate</t> (DEPC, D), 10 mM Lactate (L), the combination of DEPC and lactate (DL), or TNFα (20 ng/ml), after which p-Ser536-p65 and total p65 were detected in cell lysates. Representative western blots are shown. (D) Cal27 cells were treated as in (C) except that NF-κB activity was determined using a dual luciferase reporter assay 1, 2, and 3 h after treatment ( n = 4). (E) The basal, maximal and OXPHOS-dependent oxygen consumption rate of SiHa and MCF7 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (F) Representative western blots showing Nox1, Nox2, Nox4 expression in MCF7 cells. (G) MCF7 cells were treated for 3 h with vehicle (control, C), 1 mM of DEPC (D), 10 mM of L-Lactate (L) or combination (DL), after which p-Ser536-p65, total p65 and β-actin were detected in cell lysates. A 1-h treatment with TNFα was used as positive control. Representative western blots are shown. (H) Same as in (G) except that NF-κB activity was determined using a dual luciferase reporter assay ( n = 4). All data represent means ± SEM. * P
    Depc Treated Water, supplied by Bioneer Corporation, used in various techniques. Bioz Stars score: 90/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/depc treated water/product/Bioneer Corporation
    Average 90 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    depc treated water - by Bioz Stars, 2020-02
    90/100 stars
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    93
    Fisher Scientific depc treated water
    Inhibition of the malate-aspartate shuttle restores the ability of lactate to activate NF-κB in oxidative Cal27 tumor cells . (A) The basal, maximal and OXPHOS-dependent oxygen consumption rates of HeLa and Cal27 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (B) Representative western blots showing Nox1, Nox2, Nox4 expression in Cal27 cells. (C) Cal27 cells were treated for the indicated amounts of time with vehicle (control, C), 1 mM of diethyl <t>pyrocarbonate</t> (DEPC, D), 10 mM Lactate (L), the combination of DEPC and lactate (DL), or TNFα (20 ng/ml), after which p-Ser536-p65 and total p65 were detected in cell lysates. Representative western blots are shown. (D) Cal27 cells were treated as in (C) except that NF-κB activity was determined using a dual luciferase reporter assay 1, 2, and 3 h after treatment ( n = 4). (E) The basal, maximal and OXPHOS-dependent oxygen consumption rate of SiHa and MCF7 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (F) Representative western blots showing Nox1, Nox2, Nox4 expression in MCF7 cells. (G) MCF7 cells were treated for 3 h with vehicle (control, C), 1 mM of DEPC (D), 10 mM of L-Lactate (L) or combination (DL), after which p-Ser536-p65, total p65 and β-actin were detected in cell lysates. A 1-h treatment with TNFα was used as positive control. Representative western blots are shown. (H) Same as in (G) except that NF-κB activity was determined using a dual luciferase reporter assay ( n = 4). All data represent means ± SEM. * P
    Depc Treated Water, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 93/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/depc treated water/product/Fisher Scientific
    Average 93 stars, based on 40 article reviews
    Price from $9.99 to $1999.99
    depc treated water - by Bioz Stars, 2020-02
    93/100 stars
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    90
    Amresco depc treated water
    Inhibition of the malate-aspartate shuttle restores the ability of lactate to activate NF-κB in oxidative Cal27 tumor cells . (A) The basal, maximal and OXPHOS-dependent oxygen consumption rates of HeLa and Cal27 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (B) Representative western blots showing Nox1, Nox2, Nox4 expression in Cal27 cells. (C) Cal27 cells were treated for the indicated amounts of time with vehicle (control, C), 1 mM of diethyl <t>pyrocarbonate</t> (DEPC, D), 10 mM Lactate (L), the combination of DEPC and lactate (DL), or TNFα (20 ng/ml), after which p-Ser536-p65 and total p65 were detected in cell lysates. Representative western blots are shown. (D) Cal27 cells were treated as in (C) except that NF-κB activity was determined using a dual luciferase reporter assay 1, 2, and 3 h after treatment ( n = 4). (E) The basal, maximal and OXPHOS-dependent oxygen consumption rate of SiHa and MCF7 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (F) Representative western blots showing Nox1, Nox2, Nox4 expression in MCF7 cells. (G) MCF7 cells were treated for 3 h with vehicle (control, C), 1 mM of DEPC (D), 10 mM of L-Lactate (L) or combination (DL), after which p-Ser536-p65, total p65 and β-actin were detected in cell lysates. A 1-h treatment with TNFα was used as positive control. Representative western blots are shown. (H) Same as in (G) except that NF-κB activity was determined using a dual luciferase reporter assay ( n = 4). All data represent means ± SEM. * P
    Depc Treated Water, supplied by Amresco, used in various techniques. Bioz Stars score: 90/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/depc treated water/product/Amresco
    Average 90 stars, based on 26 article reviews
    Price from $9.99 to $1999.99
    depc treated water - by Bioz Stars, 2020-02
    90/100 stars
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    99
    Thermo Fisher depc treated water
    Inhibition of the malate-aspartate shuttle restores the ability of lactate to activate NF-κB in oxidative Cal27 tumor cells . (A) The basal, maximal and OXPHOS-dependent oxygen consumption rates of HeLa and Cal27 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (B) Representative western blots showing Nox1, Nox2, Nox4 expression in Cal27 cells. (C) Cal27 cells were treated for the indicated amounts of time with vehicle (control, C), 1 mM of diethyl <t>pyrocarbonate</t> (DEPC, D), 10 mM Lactate (L), the combination of DEPC and lactate (DL), or TNFα (20 ng/ml), after which p-Ser536-p65 and total p65 were detected in cell lysates. Representative western blots are shown. (D) Cal27 cells were treated as in (C) except that NF-κB activity was determined using a dual luciferase reporter assay 1, 2, and 3 h after treatment ( n = 4). (E) The basal, maximal and OXPHOS-dependent oxygen consumption rate of SiHa and MCF7 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (F) Representative western blots showing Nox1, Nox2, Nox4 expression in MCF7 cells. (G) MCF7 cells were treated for 3 h with vehicle (control, C), 1 mM of DEPC (D), 10 mM of L-Lactate (L) or combination (DL), after which p-Ser536-p65, total p65 and β-actin were detected in cell lysates. A 1-h treatment with TNFα was used as positive control. Representative western blots are shown. (H) Same as in (G) except that NF-κB activity was determined using a dual luciferase reporter assay ( n = 4). All data represent means ± SEM. * P
    Depc Treated Water, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2943 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/depc treated water/product/Thermo Fisher
    Average 99 stars, based on 2943 article reviews
    Price from $9.99 to $1999.99
    depc treated water - by Bioz Stars, 2020-02
    99/100 stars
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    94
    TaKaRa depc treated water
    Inhibition of the malate-aspartate shuttle restores the ability of lactate to activate NF-κB in oxidative Cal27 tumor cells . (A) The basal, maximal and OXPHOS-dependent oxygen consumption rates of HeLa and Cal27 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (B) Representative western blots showing Nox1, Nox2, Nox4 expression in Cal27 cells. (C) Cal27 cells were treated for the indicated amounts of time with vehicle (control, C), 1 mM of diethyl <t>pyrocarbonate</t> (DEPC, D), 10 mM Lactate (L), the combination of DEPC and lactate (DL), or TNFα (20 ng/ml), after which p-Ser536-p65 and total p65 were detected in cell lysates. Representative western blots are shown. (D) Cal27 cells were treated as in (C) except that NF-κB activity was determined using a dual luciferase reporter assay 1, 2, and 3 h after treatment ( n = 4). (E) The basal, maximal and OXPHOS-dependent oxygen consumption rate of SiHa and MCF7 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (F) Representative western blots showing Nox1, Nox2, Nox4 expression in MCF7 cells. (G) MCF7 cells were treated for 3 h with vehicle (control, C), 1 mM of DEPC (D), 10 mM of L-Lactate (L) or combination (DL), after which p-Ser536-p65, total p65 and β-actin were detected in cell lysates. A 1-h treatment with TNFα was used as positive control. Representative western blots are shown. (H) Same as in (G) except that NF-κB activity was determined using a dual luciferase reporter assay ( n = 4). All data represent means ± SEM. * P
    Depc Treated Water, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 124 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/depc treated water/product/TaKaRa
    Average 94 stars, based on 124 article reviews
    Price from $9.99 to $1999.99
    depc treated water - by Bioz Stars, 2020-02
    94/100 stars
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    90
    Carl Roth GmbH depc treated water
    Inhibition of the malate-aspartate shuttle restores the ability of lactate to activate NF-κB in oxidative Cal27 tumor cells . (A) The basal, maximal and OXPHOS-dependent oxygen consumption rates of HeLa and Cal27 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (B) Representative western blots showing Nox1, Nox2, Nox4 expression in Cal27 cells. (C) Cal27 cells were treated for the indicated amounts of time with vehicle (control, C), 1 mM of diethyl <t>pyrocarbonate</t> (DEPC, D), 10 mM Lactate (L), the combination of DEPC and lactate (DL), or TNFα (20 ng/ml), after which p-Ser536-p65 and total p65 were detected in cell lysates. Representative western blots are shown. (D) Cal27 cells were treated as in (C) except that NF-κB activity was determined using a dual luciferase reporter assay 1, 2, and 3 h after treatment ( n = 4). (E) The basal, maximal and OXPHOS-dependent oxygen consumption rate of SiHa and MCF7 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (F) Representative western blots showing Nox1, Nox2, Nox4 expression in MCF7 cells. (G) MCF7 cells were treated for 3 h with vehicle (control, C), 1 mM of DEPC (D), 10 mM of L-Lactate (L) or combination (DL), after which p-Ser536-p65, total p65 and β-actin were detected in cell lysates. A 1-h treatment with TNFα was used as positive control. Representative western blots are shown. (H) Same as in (G) except that NF-κB activity was determined using a dual luciferase reporter assay ( n = 4). All data represent means ± SEM. * P
    Depc Treated Water, supplied by Carl Roth GmbH, used in various techniques. Bioz Stars score: 90/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/depc treated water/product/Carl Roth GmbH
    Average 90 stars, based on 26 article reviews
    Price from $9.99 to $1999.99
    depc treated water - by Bioz Stars, 2020-02
    90/100 stars
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    94
    Santa Cruz Biotechnology depc treated water
    Inhibition of the malate-aspartate shuttle restores the ability of lactate to activate NF-κB in oxidative Cal27 tumor cells . (A) The basal, maximal and OXPHOS-dependent oxygen consumption rates of HeLa and Cal27 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (B) Representative western blots showing Nox1, Nox2, Nox4 expression in Cal27 cells. (C) Cal27 cells were treated for the indicated amounts of time with vehicle (control, C), 1 mM of diethyl <t>pyrocarbonate</t> (DEPC, D), 10 mM Lactate (L), the combination of DEPC and lactate (DL), or TNFα (20 ng/ml), after which p-Ser536-p65 and total p65 were detected in cell lysates. Representative western blots are shown. (D) Cal27 cells were treated as in (C) except that NF-κB activity was determined using a dual luciferase reporter assay 1, 2, and 3 h after treatment ( n = 4). (E) The basal, maximal and OXPHOS-dependent oxygen consumption rate of SiHa and MCF7 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (F) Representative western blots showing Nox1, Nox2, Nox4 expression in MCF7 cells. (G) MCF7 cells were treated for 3 h with vehicle (control, C), 1 mM of DEPC (D), 10 mM of L-Lactate (L) or combination (DL), after which p-Ser536-p65, total p65 and β-actin were detected in cell lysates. A 1-h treatment with TNFα was used as positive control. Representative western blots are shown. (H) Same as in (G) except that NF-κB activity was determined using a dual luciferase reporter assay ( n = 4). All data represent means ± SEM. * P
    Depc Treated Water, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/depc treated water/product/Santa Cruz Biotechnology
    Average 94 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    depc treated water - by Bioz Stars, 2020-02
    94/100 stars
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    92
    G Biosciences depc treated water
    Inhibition of the malate-aspartate shuttle restores the ability of lactate to activate NF-κB in oxidative Cal27 tumor cells . (A) The basal, maximal and OXPHOS-dependent oxygen consumption rates of HeLa and Cal27 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (B) Representative western blots showing Nox1, Nox2, Nox4 expression in Cal27 cells. (C) Cal27 cells were treated for the indicated amounts of time with vehicle (control, C), 1 mM of diethyl <t>pyrocarbonate</t> (DEPC, D), 10 mM Lactate (L), the combination of DEPC and lactate (DL), or TNFα (20 ng/ml), after which p-Ser536-p65 and total p65 were detected in cell lysates. Representative western blots are shown. (D) Cal27 cells were treated as in (C) except that NF-κB activity was determined using a dual luciferase reporter assay 1, 2, and 3 h after treatment ( n = 4). (E) The basal, maximal and OXPHOS-dependent oxygen consumption rate of SiHa and MCF7 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (F) Representative western blots showing Nox1, Nox2, Nox4 expression in MCF7 cells. (G) MCF7 cells were treated for 3 h with vehicle (control, C), 1 mM of DEPC (D), 10 mM of L-Lactate (L) or combination (DL), after which p-Ser536-p65, total p65 and β-actin were detected in cell lysates. A 1-h treatment with TNFα was used as positive control. Representative western blots are shown. (H) Same as in (G) except that NF-κB activity was determined using a dual luciferase reporter assay ( n = 4). All data represent means ± SEM. * P
    Depc Treated Water, supplied by G Biosciences, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/depc treated water/product/G Biosciences
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    depc treated water - by Bioz Stars, 2020-02
    92/100 stars
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    90
    Biological Industries Inc depc treated water
    Inhibition of the malate-aspartate shuttle restores the ability of lactate to activate NF-κB in oxidative Cal27 tumor cells . (A) The basal, maximal and OXPHOS-dependent oxygen consumption rates of HeLa and Cal27 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (B) Representative western blots showing Nox1, Nox2, Nox4 expression in Cal27 cells. (C) Cal27 cells were treated for the indicated amounts of time with vehicle (control, C), 1 mM of diethyl <t>pyrocarbonate</t> (DEPC, D), 10 mM Lactate (L), the combination of DEPC and lactate (DL), or TNFα (20 ng/ml), after which p-Ser536-p65 and total p65 were detected in cell lysates. Representative western blots are shown. (D) Cal27 cells were treated as in (C) except that NF-κB activity was determined using a dual luciferase reporter assay 1, 2, and 3 h after treatment ( n = 4). (E) The basal, maximal and OXPHOS-dependent oxygen consumption rate of SiHa and MCF7 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (F) Representative western blots showing Nox1, Nox2, Nox4 expression in MCF7 cells. (G) MCF7 cells were treated for 3 h with vehicle (control, C), 1 mM of DEPC (D), 10 mM of L-Lactate (L) or combination (DL), after which p-Ser536-p65, total p65 and β-actin were detected in cell lysates. A 1-h treatment with TNFα was used as positive control. Representative western blots are shown. (H) Same as in (G) except that NF-κB activity was determined using a dual luciferase reporter assay ( n = 4). All data represent means ± SEM. * P
    Depc Treated Water, supplied by Biological Industries Inc, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/depc treated water/product/Biological Industries Inc
    Average 90 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    depc treated water - by Bioz Stars, 2020-02
    90/100 stars
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    94
    BioExpress depc treated water
    Inhibition of the malate-aspartate shuttle restores the ability of lactate to activate NF-κB in oxidative Cal27 tumor cells . (A) The basal, maximal and OXPHOS-dependent oxygen consumption rates of HeLa and Cal27 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (B) Representative western blots showing Nox1, Nox2, Nox4 expression in Cal27 cells. (C) Cal27 cells were treated for the indicated amounts of time with vehicle (control, C), 1 mM of diethyl <t>pyrocarbonate</t> (DEPC, D), 10 mM Lactate (L), the combination of DEPC and lactate (DL), or TNFα (20 ng/ml), after which p-Ser536-p65 and total p65 were detected in cell lysates. Representative western blots are shown. (D) Cal27 cells were treated as in (C) except that NF-κB activity was determined using a dual luciferase reporter assay 1, 2, and 3 h after treatment ( n = 4). (E) The basal, maximal and OXPHOS-dependent oxygen consumption rate of SiHa and MCF7 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (F) Representative western blots showing Nox1, Nox2, Nox4 expression in MCF7 cells. (G) MCF7 cells were treated for 3 h with vehicle (control, C), 1 mM of DEPC (D), 10 mM of L-Lactate (L) or combination (DL), after which p-Ser536-p65, total p65 and β-actin were detected in cell lysates. A 1-h treatment with TNFα was used as positive control. Representative western blots are shown. (H) Same as in (G) except that NF-κB activity was determined using a dual luciferase reporter assay ( n = 4). All data represent means ± SEM. * P
    Depc Treated Water, supplied by BioExpress, used in various techniques. Bioz Stars score: 94/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/depc treated water/product/BioExpress
    Average 94 stars, based on 30 article reviews
    Price from $9.99 to $1999.99
    depc treated water - by Bioz Stars, 2020-02
    94/100 stars
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    90
    Severn Biotech Limited depc treated water
    Inhibition of the malate-aspartate shuttle restores the ability of lactate to activate NF-κB in oxidative Cal27 tumor cells . (A) The basal, maximal and OXPHOS-dependent oxygen consumption rates of HeLa and Cal27 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (B) Representative western blots showing Nox1, Nox2, Nox4 expression in Cal27 cells. (C) Cal27 cells were treated for the indicated amounts of time with vehicle (control, C), 1 mM of diethyl <t>pyrocarbonate</t> (DEPC, D), 10 mM Lactate (L), the combination of DEPC and lactate (DL), or TNFα (20 ng/ml), after which p-Ser536-p65 and total p65 were detected in cell lysates. Representative western blots are shown. (D) Cal27 cells were treated as in (C) except that NF-κB activity was determined using a dual luciferase reporter assay 1, 2, and 3 h after treatment ( n = 4). (E) The basal, maximal and OXPHOS-dependent oxygen consumption rate of SiHa and MCF7 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (F) Representative western blots showing Nox1, Nox2, Nox4 expression in MCF7 cells. (G) MCF7 cells were treated for 3 h with vehicle (control, C), 1 mM of DEPC (D), 10 mM of L-Lactate (L) or combination (DL), after which p-Ser536-p65, total p65 and β-actin were detected in cell lysates. A 1-h treatment with TNFα was used as positive control. Representative western blots are shown. (H) Same as in (G) except that NF-κB activity was determined using a dual luciferase reporter assay ( n = 4). All data represent means ± SEM. * P
    Depc Treated Water, supplied by Severn Biotech Limited, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 1 article reviews
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    depc treated water - by Bioz Stars, 2020-02
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    93
    Nacalai depc treated water
    Inhibition of the malate-aspartate shuttle restores the ability of lactate to activate NF-κB in oxidative Cal27 tumor cells . (A) The basal, maximal and OXPHOS-dependent oxygen consumption rates of HeLa and Cal27 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (B) Representative western blots showing Nox1, Nox2, Nox4 expression in Cal27 cells. (C) Cal27 cells were treated for the indicated amounts of time with vehicle (control, C), 1 mM of diethyl <t>pyrocarbonate</t> (DEPC, D), 10 mM Lactate (L), the combination of DEPC and lactate (DL), or TNFα (20 ng/ml), after which p-Ser536-p65 and total p65 were detected in cell lysates. Representative western blots are shown. (D) Cal27 cells were treated as in (C) except that NF-κB activity was determined using a dual luciferase reporter assay 1, 2, and 3 h after treatment ( n = 4). (E) The basal, maximal and OXPHOS-dependent oxygen consumption rate of SiHa and MCF7 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (F) Representative western blots showing Nox1, Nox2, Nox4 expression in MCF7 cells. (G) MCF7 cells were treated for 3 h with vehicle (control, C), 1 mM of DEPC (D), 10 mM of L-Lactate (L) or combination (DL), after which p-Ser536-p65, total p65 and β-actin were detected in cell lysates. A 1-h treatment with TNFα was used as positive control. Representative western blots are shown. (H) Same as in (G) except that NF-κB activity was determined using a dual luciferase reporter assay ( n = 4). All data represent means ± SEM. * P
    Depc Treated Water, supplied by Nacalai, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    depc treated water - by Bioz Stars, 2020-02
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    90
    Quality Biological Inc depc treated water
    Inhibition of the malate-aspartate shuttle restores the ability of lactate to activate NF-κB in oxidative Cal27 tumor cells . (A) The basal, maximal and OXPHOS-dependent oxygen consumption rates of HeLa and Cal27 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (B) Representative western blots showing Nox1, Nox2, Nox4 expression in Cal27 cells. (C) Cal27 cells were treated for the indicated amounts of time with vehicle (control, C), 1 mM of diethyl <t>pyrocarbonate</t> (DEPC, D), 10 mM Lactate (L), the combination of DEPC and lactate (DL), or TNFα (20 ng/ml), after which p-Ser536-p65 and total p65 were detected in cell lysates. Representative western blots are shown. (D) Cal27 cells were treated as in (C) except that NF-κB activity was determined using a dual luciferase reporter assay 1, 2, and 3 h after treatment ( n = 4). (E) The basal, maximal and OXPHOS-dependent oxygen consumption rate of SiHa and MCF7 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (F) Representative western blots showing Nox1, Nox2, Nox4 expression in MCF7 cells. (G) MCF7 cells were treated for 3 h with vehicle (control, C), 1 mM of DEPC (D), 10 mM of L-Lactate (L) or combination (DL), after which p-Ser536-p65, total p65 and β-actin were detected in cell lysates. A 1-h treatment with TNFα was used as positive control. Representative western blots are shown. (H) Same as in (G) except that NF-κB activity was determined using a dual luciferase reporter assay ( n = 4). All data represent means ± SEM. * P
    Depc Treated Water, supplied by Quality Biological Inc, used in various techniques. Bioz Stars score: 90/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    depc treated water - by Bioz Stars, 2020-02
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    90
    CinnaGen Co depc treated water
    Inhibition of the malate-aspartate shuttle restores the ability of lactate to activate NF-κB in oxidative Cal27 tumor cells . (A) The basal, maximal and OXPHOS-dependent oxygen consumption rates of HeLa and Cal27 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (B) Representative western blots showing Nox1, Nox2, Nox4 expression in Cal27 cells. (C) Cal27 cells were treated for the indicated amounts of time with vehicle (control, C), 1 mM of diethyl <t>pyrocarbonate</t> (DEPC, D), 10 mM Lactate (L), the combination of DEPC and lactate (DL), or TNFα (20 ng/ml), after which p-Ser536-p65 and total p65 were detected in cell lysates. Representative western blots are shown. (D) Cal27 cells were treated as in (C) except that NF-κB activity was determined using a dual luciferase reporter assay 1, 2, and 3 h after treatment ( n = 4). (E) The basal, maximal and OXPHOS-dependent oxygen consumption rate of SiHa and MCF7 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (F) Representative western blots showing Nox1, Nox2, Nox4 expression in MCF7 cells. (G) MCF7 cells were treated for 3 h with vehicle (control, C), 1 mM of DEPC (D), 10 mM of L-Lactate (L) or combination (DL), after which p-Ser536-p65, total p65 and β-actin were detected in cell lysates. A 1-h treatment with TNFα was used as positive control. Representative western blots are shown. (H) Same as in (G) except that NF-κB activity was determined using a dual luciferase reporter assay ( n = 4). All data represent means ± SEM. * P
    Depc Treated Water, supplied by CinnaGen Co, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    depc treated water - by Bioz Stars, 2020-02
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    90
    iNtRON Biotechnology depc treated water
    Inhibition of the malate-aspartate shuttle restores the ability of lactate to activate NF-κB in oxidative Cal27 tumor cells . (A) The basal, maximal and OXPHOS-dependent oxygen consumption rates of HeLa and Cal27 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (B) Representative western blots showing Nox1, Nox2, Nox4 expression in Cal27 cells. (C) Cal27 cells were treated for the indicated amounts of time with vehicle (control, C), 1 mM of diethyl <t>pyrocarbonate</t> (DEPC, D), 10 mM Lactate (L), the combination of DEPC and lactate (DL), or TNFα (20 ng/ml), after which p-Ser536-p65 and total p65 were detected in cell lysates. Representative western blots are shown. (D) Cal27 cells were treated as in (C) except that NF-κB activity was determined using a dual luciferase reporter assay 1, 2, and 3 h after treatment ( n = 4). (E) The basal, maximal and OXPHOS-dependent oxygen consumption rate of SiHa and MCF7 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (F) Representative western blots showing Nox1, Nox2, Nox4 expression in MCF7 cells. (G) MCF7 cells were treated for 3 h with vehicle (control, C), 1 mM of DEPC (D), 10 mM of L-Lactate (L) or combination (DL), after which p-Ser536-p65, total p65 and β-actin were detected in cell lysates. A 1-h treatment with TNFα was used as positive control. Representative western blots are shown. (H) Same as in (G) except that NF-κB activity was determined using a dual luciferase reporter assay ( n = 4). All data represent means ± SEM. * P
    Depc Treated Water, supplied by iNtRON Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    depc treated water - by Bioz Stars, 2020-02
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    90
    Beyotime depc treated water
    Inhibition of the malate-aspartate shuttle restores the ability of lactate to activate NF-κB in oxidative Cal27 tumor cells . (A) The basal, maximal and OXPHOS-dependent oxygen consumption rates of HeLa and Cal27 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (B) Representative western blots showing Nox1, Nox2, Nox4 expression in Cal27 cells. (C) Cal27 cells were treated for the indicated amounts of time with vehicle (control, C), 1 mM of diethyl <t>pyrocarbonate</t> (DEPC, D), 10 mM Lactate (L), the combination of DEPC and lactate (DL), or TNFα (20 ng/ml), after which p-Ser536-p65 and total p65 were detected in cell lysates. Representative western blots are shown. (D) Cal27 cells were treated as in (C) except that NF-κB activity was determined using a dual luciferase reporter assay 1, 2, and 3 h after treatment ( n = 4). (E) The basal, maximal and OXPHOS-dependent oxygen consumption rate of SiHa and MCF7 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (F) Representative western blots showing Nox1, Nox2, Nox4 expression in MCF7 cells. (G) MCF7 cells were treated for 3 h with vehicle (control, C), 1 mM of DEPC (D), 10 mM of L-Lactate (L) or combination (DL), after which p-Ser536-p65, total p65 and β-actin were detected in cell lysates. A 1-h treatment with TNFα was used as positive control. Representative western blots are shown. (H) Same as in (G) except that NF-κB activity was determined using a dual luciferase reporter assay ( n = 4). All data represent means ± SEM. * P
    Depc Treated Water, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    depc treated water - by Bioz Stars, 2020-02
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    94
    Meridian Life Science depc treated water
    Inhibition of the malate-aspartate shuttle restores the ability of lactate to activate NF-κB in oxidative Cal27 tumor cells . (A) The basal, maximal and OXPHOS-dependent oxygen consumption rates of HeLa and Cal27 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (B) Representative western blots showing Nox1, Nox2, Nox4 expression in Cal27 cells. (C) Cal27 cells were treated for the indicated amounts of time with vehicle (control, C), 1 mM of diethyl <t>pyrocarbonate</t> (DEPC, D), 10 mM Lactate (L), the combination of DEPC and lactate (DL), or TNFα (20 ng/ml), after which p-Ser536-p65 and total p65 were detected in cell lysates. Representative western blots are shown. (D) Cal27 cells were treated as in (C) except that NF-κB activity was determined using a dual luciferase reporter assay 1, 2, and 3 h after treatment ( n = 4). (E) The basal, maximal and OXPHOS-dependent oxygen consumption rate of SiHa and MCF7 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (F) Representative western blots showing Nox1, Nox2, Nox4 expression in MCF7 cells. (G) MCF7 cells were treated for 3 h with vehicle (control, C), 1 mM of DEPC (D), 10 mM of L-Lactate (L) or combination (DL), after which p-Ser536-p65, total p65 and β-actin were detected in cell lysates. A 1-h treatment with TNFα was used as positive control. Representative western blots are shown. (H) Same as in (G) except that NF-κB activity was determined using a dual luciferase reporter assay ( n = 4). All data represent means ± SEM. * P
    Depc Treated Water, supplied by Meridian Life Science, used in various techniques. Bioz Stars score: 94/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    depc treated water - by Bioz Stars, 2020-02
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    95
    Thermo Fisher nuclease free water not depc treated
    Inhibition of the malate-aspartate shuttle restores the ability of lactate to activate NF-κB in oxidative Cal27 tumor cells . (A) The basal, maximal and OXPHOS-dependent oxygen consumption rates of HeLa and Cal27 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (B) Representative western blots showing Nox1, Nox2, Nox4 expression in Cal27 cells. (C) Cal27 cells were treated for the indicated amounts of time with vehicle (control, C), 1 mM of diethyl <t>pyrocarbonate</t> (DEPC, D), 10 mM Lactate (L), the combination of DEPC and lactate (DL), or TNFα (20 ng/ml), after which p-Ser536-p65 and total p65 were detected in cell lysates. Representative western blots are shown. (D) Cal27 cells were treated as in (C) except that NF-κB activity was determined using a dual luciferase reporter assay 1, 2, and 3 h after treatment ( n = 4). (E) The basal, maximal and OXPHOS-dependent oxygen consumption rate of SiHa and MCF7 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (F) Representative western blots showing Nox1, Nox2, Nox4 expression in MCF7 cells. (G) MCF7 cells were treated for 3 h with vehicle (control, C), 1 mM of DEPC (D), 10 mM of L-Lactate (L) or combination (DL), after which p-Ser536-p65, total p65 and β-actin were detected in cell lysates. A 1-h treatment with TNFα was used as positive control. Representative western blots are shown. (H) Same as in (G) except that NF-κB activity was determined using a dual luciferase reporter assay ( n = 4). All data represent means ± SEM. * P
    Nuclease Free Water Not Depc Treated, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 521 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nuclease free water not depc treated - by Bioz Stars, 2020-02
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    91
    Applichem depc diethylpyrocarbonate treated water
    Inhibition of the malate-aspartate shuttle restores the ability of lactate to activate NF-κB in oxidative Cal27 tumor cells . (A) The basal, maximal and OXPHOS-dependent oxygen consumption rates of HeLa and Cal27 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (B) Representative western blots showing Nox1, Nox2, Nox4 expression in Cal27 cells. (C) Cal27 cells were treated for the indicated amounts of time with vehicle (control, C), 1 mM of diethyl <t>pyrocarbonate</t> (DEPC, D), 10 mM Lactate (L), the combination of DEPC and lactate (DL), or TNFα (20 ng/ml), after which p-Ser536-p65 and total p65 were detected in cell lysates. Representative western blots are shown. (D) Cal27 cells were treated as in (C) except that NF-κB activity was determined using a dual luciferase reporter assay 1, 2, and 3 h after treatment ( n = 4). (E) The basal, maximal and OXPHOS-dependent oxygen consumption rate of SiHa and MCF7 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (F) Representative western blots showing Nox1, Nox2, Nox4 expression in MCF7 cells. (G) MCF7 cells were treated for 3 h with vehicle (control, C), 1 mM of DEPC (D), 10 mM of L-Lactate (L) or combination (DL), after which p-Ser536-p65, total p65 and β-actin were detected in cell lysates. A 1-h treatment with TNFα was used as positive control. Representative western blots are shown. (H) Same as in (G) except that NF-κB activity was determined using a dual luciferase reporter assay ( n = 4). All data represent means ± SEM. * P
    Depc Diethylpyrocarbonate Treated Water, supplied by Applichem, used in various techniques. Bioz Stars score: 91/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    depc diethylpyrocarbonate treated water - by Bioz Stars, 2020-02
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    86
    Thermo Fisher depc diethylpyrocarbonate treated water
    Inhibition of the malate-aspartate shuttle restores the ability of lactate to activate NF-κB in oxidative Cal27 tumor cells . (A) The basal, maximal and OXPHOS-dependent oxygen consumption rates of HeLa and Cal27 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (B) Representative western blots showing Nox1, Nox2, Nox4 expression in Cal27 cells. (C) Cal27 cells were treated for the indicated amounts of time with vehicle (control, C), 1 mM of diethyl <t>pyrocarbonate</t> (DEPC, D), 10 mM Lactate (L), the combination of DEPC and lactate (DL), or TNFα (20 ng/ml), after which p-Ser536-p65 and total p65 were detected in cell lysates. Representative western blots are shown. (D) Cal27 cells were treated as in (C) except that NF-κB activity was determined using a dual luciferase reporter assay 1, 2, and 3 h after treatment ( n = 4). (E) The basal, maximal and OXPHOS-dependent oxygen consumption rate of SiHa and MCF7 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (F) Representative western blots showing Nox1, Nox2, Nox4 expression in MCF7 cells. (G) MCF7 cells were treated for 3 h with vehicle (control, C), 1 mM of DEPC (D), 10 mM of L-Lactate (L) or combination (DL), after which p-Ser536-p65, total p65 and β-actin were detected in cell lysates. A 1-h treatment with TNFα was used as positive control. Representative western blots are shown. (H) Same as in (G) except that NF-κB activity was determined using a dual luciferase reporter assay ( n = 4). All data represent means ± SEM. * P
    Depc Diethylpyrocarbonate Treated Water, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    87
    Keygen Biotech diethylpyrocarbonate depc treated water
    Inhibition of the malate-aspartate shuttle restores the ability of lactate to activate NF-κB in oxidative Cal27 tumor cells . (A) The basal, maximal and OXPHOS-dependent oxygen consumption rates of HeLa and Cal27 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (B) Representative western blots showing Nox1, Nox2, Nox4 expression in Cal27 cells. (C) Cal27 cells were treated for the indicated amounts of time with vehicle (control, C), 1 mM of diethyl <t>pyrocarbonate</t> (DEPC, D), 10 mM Lactate (L), the combination of DEPC and lactate (DL), or TNFα (20 ng/ml), after which p-Ser536-p65 and total p65 were detected in cell lysates. Representative western blots are shown. (D) Cal27 cells were treated as in (C) except that NF-κB activity was determined using a dual luciferase reporter assay 1, 2, and 3 h after treatment ( n = 4). (E) The basal, maximal and OXPHOS-dependent oxygen consumption rate of SiHa and MCF7 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (F) Representative western blots showing Nox1, Nox2, Nox4 expression in MCF7 cells. (G) MCF7 cells were treated for 3 h with vehicle (control, C), 1 mM of DEPC (D), 10 mM of L-Lactate (L) or combination (DL), after which p-Ser536-p65, total p65 and β-actin were detected in cell lysates. A 1-h treatment with TNFα was used as positive control. Representative western blots are shown. (H) Same as in (G) except that NF-κB activity was determined using a dual luciferase reporter assay ( n = 4). All data represent means ± SEM. * P
    Diethylpyrocarbonate Depc Treated Water, supplied by Keygen Biotech, used in various techniques. Bioz Stars score: 87/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Millipore diethylpyrocarbonate depc treated water
    Inhibition of the malate-aspartate shuttle restores the ability of lactate to activate NF-κB in oxidative Cal27 tumor cells . (A) The basal, maximal and OXPHOS-dependent oxygen consumption rates of HeLa and Cal27 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (B) Representative western blots showing Nox1, Nox2, Nox4 expression in Cal27 cells. (C) Cal27 cells were treated for the indicated amounts of time with vehicle (control, C), 1 mM of diethyl <t>pyrocarbonate</t> (DEPC, D), 10 mM Lactate (L), the combination of DEPC and lactate (DL), or TNFα (20 ng/ml), after which p-Ser536-p65 and total p65 were detected in cell lysates. Representative western blots are shown. (D) Cal27 cells were treated as in (C) except that NF-κB activity was determined using a dual luciferase reporter assay 1, 2, and 3 h after treatment ( n = 4). (E) The basal, maximal and OXPHOS-dependent oxygen consumption rate of SiHa and MCF7 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (F) Representative western blots showing Nox1, Nox2, Nox4 expression in MCF7 cells. (G) MCF7 cells were treated for 3 h with vehicle (control, C), 1 mM of DEPC (D), 10 mM of L-Lactate (L) or combination (DL), after which p-Ser536-p65, total p65 and β-actin were detected in cell lysates. A 1-h treatment with TNFα was used as positive control. Representative western blots are shown. (H) Same as in (G) except that NF-κB activity was determined using a dual luciferase reporter assay ( n = 4). All data represent means ± SEM. * P
    Diethylpyrocarbonate Depc Treated Water, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    diethylpyrocarbonate depc treated water - by Bioz Stars, 2020-02
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    78
    Fisher Scientific depc treated nuclease free water
    Inhibition of the malate-aspartate shuttle restores the ability of lactate to activate NF-κB in oxidative Cal27 tumor cells . (A) The basal, maximal and OXPHOS-dependent oxygen consumption rates of HeLa and Cal27 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (B) Representative western blots showing Nox1, Nox2, Nox4 expression in Cal27 cells. (C) Cal27 cells were treated for the indicated amounts of time with vehicle (control, C), 1 mM of diethyl <t>pyrocarbonate</t> (DEPC, D), 10 mM Lactate (L), the combination of DEPC and lactate (DL), or TNFα (20 ng/ml), after which p-Ser536-p65 and total p65 were detected in cell lysates. Representative western blots are shown. (D) Cal27 cells were treated as in (C) except that NF-κB activity was determined using a dual luciferase reporter assay 1, 2, and 3 h after treatment ( n = 4). (E) The basal, maximal and OXPHOS-dependent oxygen consumption rate of SiHa and MCF7 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (F) Representative western blots showing Nox1, Nox2, Nox4 expression in MCF7 cells. (G) MCF7 cells were treated for 3 h with vehicle (control, C), 1 mM of DEPC (D), 10 mM of L-Lactate (L) or combination (DL), after which p-Ser536-p65, total p65 and β-actin were detected in cell lysates. A 1-h treatment with TNFα was used as positive control. Representative western blots are shown. (H) Same as in (G) except that NF-κB activity was determined using a dual luciferase reporter assay ( n = 4). All data represent means ± SEM. * P
    Depc Treated Nuclease Free Water, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 78/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    depc treated nuclease free water - by Bioz Stars, 2020-02
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    90
    Beijing Solarbio Science diethylpyrocarbonate depc treated water
    Inhibition of the malate-aspartate shuttle restores the ability of lactate to activate NF-κB in oxidative Cal27 tumor cells . (A) The basal, maximal and OXPHOS-dependent oxygen consumption rates of HeLa and Cal27 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (B) Representative western blots showing Nox1, Nox2, Nox4 expression in Cal27 cells. (C) Cal27 cells were treated for the indicated amounts of time with vehicle (control, C), 1 mM of diethyl <t>pyrocarbonate</t> (DEPC, D), 10 mM Lactate (L), the combination of DEPC and lactate (DL), or TNFα (20 ng/ml), after which p-Ser536-p65 and total p65 were detected in cell lysates. Representative western blots are shown. (D) Cal27 cells were treated as in (C) except that NF-κB activity was determined using a dual luciferase reporter assay 1, 2, and 3 h after treatment ( n = 4). (E) The basal, maximal and OXPHOS-dependent oxygen consumption rate of SiHa and MCF7 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (F) Representative western blots showing Nox1, Nox2, Nox4 expression in MCF7 cells. (G) MCF7 cells were treated for 3 h with vehicle (control, C), 1 mM of DEPC (D), 10 mM of L-Lactate (L) or combination (DL), after which p-Ser536-p65, total p65 and β-actin were detected in cell lysates. A 1-h treatment with TNFα was used as positive control. Representative western blots are shown. (H) Same as in (G) except that NF-κB activity was determined using a dual luciferase reporter assay ( n = 4). All data represent means ± SEM. * P
    Diethylpyrocarbonate Depc Treated Water, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/diethylpyrocarbonate depc treated water/product/Beijing Solarbio Science
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    diethylpyrocarbonate depc treated water - by Bioz Stars, 2020-02
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    92
    Sangon Biotech diethylpyrocarbonate depc treated water
    Inhibition of the malate-aspartate shuttle restores the ability of lactate to activate NF-κB in oxidative Cal27 tumor cells . (A) The basal, maximal and OXPHOS-dependent oxygen consumption rates of HeLa and Cal27 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (B) Representative western blots showing Nox1, Nox2, Nox4 expression in Cal27 cells. (C) Cal27 cells were treated for the indicated amounts of time with vehicle (control, C), 1 mM of diethyl <t>pyrocarbonate</t> (DEPC, D), 10 mM Lactate (L), the combination of DEPC and lactate (DL), or TNFα (20 ng/ml), after which p-Ser536-p65 and total p65 were detected in cell lysates. Representative western blots are shown. (D) Cal27 cells were treated as in (C) except that NF-κB activity was determined using a dual luciferase reporter assay 1, 2, and 3 h after treatment ( n = 4). (E) The basal, maximal and OXPHOS-dependent oxygen consumption rate of SiHa and MCF7 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (F) Representative western blots showing Nox1, Nox2, Nox4 expression in MCF7 cells. (G) MCF7 cells were treated for 3 h with vehicle (control, C), 1 mM of DEPC (D), 10 mM of L-Lactate (L) or combination (DL), after which p-Ser536-p65, total p65 and β-actin were detected in cell lysates. A 1-h treatment with TNFα was used as positive control. Representative western blots are shown. (H) Same as in (G) except that NF-κB activity was determined using a dual luciferase reporter assay ( n = 4). All data represent means ± SEM. * P
    Diethylpyrocarbonate Depc Treated Water, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/diethylpyrocarbonate depc treated water/product/Sangon Biotech
    Average 92 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    diethylpyrocarbonate depc treated water - by Bioz Stars, 2020-02
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    92
    iNtRON Biotechnology diethylpyrocarbonate depc treated water
    Inhibition of the malate-aspartate shuttle restores the ability of lactate to activate NF-κB in oxidative Cal27 tumor cells . (A) The basal, maximal and OXPHOS-dependent oxygen consumption rates of HeLa and Cal27 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (B) Representative western blots showing Nox1, Nox2, Nox4 expression in Cal27 cells. (C) Cal27 cells were treated for the indicated amounts of time with vehicle (control, C), 1 mM of diethyl <t>pyrocarbonate</t> (DEPC, D), 10 mM Lactate (L), the combination of DEPC and lactate (DL), or TNFα (20 ng/ml), after which p-Ser536-p65 and total p65 were detected in cell lysates. Representative western blots are shown. (D) Cal27 cells were treated as in (C) except that NF-κB activity was determined using a dual luciferase reporter assay 1, 2, and 3 h after treatment ( n = 4). (E) The basal, maximal and OXPHOS-dependent oxygen consumption rate of SiHa and MCF7 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (F) Representative western blots showing Nox1, Nox2, Nox4 expression in MCF7 cells. (G) MCF7 cells were treated for 3 h with vehicle (control, C), 1 mM of DEPC (D), 10 mM of L-Lactate (L) or combination (DL), after which p-Ser536-p65, total p65 and β-actin were detected in cell lysates. A 1-h treatment with TNFα was used as positive control. Representative western blots are shown. (H) Same as in (G) except that NF-κB activity was determined using a dual luciferase reporter assay ( n = 4). All data represent means ± SEM. * P
    Diethylpyrocarbonate Depc Treated Water, supplied by iNtRON Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/diethylpyrocarbonate depc treated water/product/iNtRON Biotechnology
    Average 92 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    diethylpyrocarbonate depc treated water - by Bioz Stars, 2020-02
    92/100 stars
      Buy from Supplier

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    Inhibition of the malate-aspartate shuttle restores the ability of lactate to activate NF-κB in oxidative Cal27 tumor cells . (A) The basal, maximal and OXPHOS-dependent oxygen consumption rates of HeLa and Cal27 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (B) Representative western blots showing Nox1, Nox2, Nox4 expression in Cal27 cells. (C) Cal27 cells were treated for the indicated amounts of time with vehicle (control, C), 1 mM of diethyl pyrocarbonate (DEPC, D), 10 mM Lactate (L), the combination of DEPC and lactate (DL), or TNFα (20 ng/ml), after which p-Ser536-p65 and total p65 were detected in cell lysates. Representative western blots are shown. (D) Cal27 cells were treated as in (C) except that NF-κB activity was determined using a dual luciferase reporter assay 1, 2, and 3 h after treatment ( n = 4). (E) The basal, maximal and OXPHOS-dependent oxygen consumption rate of SiHa and MCF7 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (F) Representative western blots showing Nox1, Nox2, Nox4 expression in MCF7 cells. (G) MCF7 cells were treated for 3 h with vehicle (control, C), 1 mM of DEPC (D), 10 mM of L-Lactate (L) or combination (DL), after which p-Ser536-p65, total p65 and β-actin were detected in cell lysates. A 1-h treatment with TNFα was used as positive control. Representative western blots are shown. (H) Same as in (G) except that NF-κB activity was determined using a dual luciferase reporter assay ( n = 4). All data represent means ± SEM. * P

    Journal: Frontiers in Pharmacology

    Article Title: Lactate does not activate NF-κB in oxidative tumor cells

    doi: 10.3389/fphar.2015.00228

    Figure Lengend Snippet: Inhibition of the malate-aspartate shuttle restores the ability of lactate to activate NF-κB in oxidative Cal27 tumor cells . (A) The basal, maximal and OXPHOS-dependent oxygen consumption rates of HeLa and Cal27 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (B) Representative western blots showing Nox1, Nox2, Nox4 expression in Cal27 cells. (C) Cal27 cells were treated for the indicated amounts of time with vehicle (control, C), 1 mM of diethyl pyrocarbonate (DEPC, D), 10 mM Lactate (L), the combination of DEPC and lactate (DL), or TNFα (20 ng/ml), after which p-Ser536-p65 and total p65 were detected in cell lysates. Representative western blots are shown. (D) Cal27 cells were treated as in (C) except that NF-κB activity was determined using a dual luciferase reporter assay 1, 2, and 3 h after treatment ( n = 4). (E) The basal, maximal and OXPHOS-dependent oxygen consumption rate of SiHa and MCF7 human tumor cells were determined using Seahorse bioanalysis ( n ≥6). (F) Representative western blots showing Nox1, Nox2, Nox4 expression in MCF7 cells. (G) MCF7 cells were treated for 3 h with vehicle (control, C), 1 mM of DEPC (D), 10 mM of L-Lactate (L) or combination (DL), after which p-Ser536-p65, total p65 and β-actin were detected in cell lysates. A 1-h treatment with TNFα was used as positive control. Representative western blots are shown. (H) Same as in (G) except that NF-κB activity was determined using a dual luciferase reporter assay ( n = 4). All data represent means ± SEM. * P

    Article Snippet: Where indicated, cells in fresh medium were treated with human TNFα (Miltenyi), sodium L -lactate (Sigma), N -acetyl-L -cysteine (NAC, Sigma), H2 O2 (Sigma), rotenone (Sigma) or diethyl pyrocarbonate (DEPC, Sigma).

    Techniques: Inhibition, Western Blot, Expressing, Activity Assay, Luciferase, Reporter Assay, Positive Control

    Inhibition of the malate-aspartate shuttle restores the ability of lactate to activate NF-κB in oxidative HeLa tumor cells . (A,B) NF-κB activity was determined using a dual luciferase reporter assay. HeLa ( A , N = 3; n = 12), SiHa ( B , N = 3; n = 12) cells were treated for the indicated amounts of time with vehicle (control), 10 mM of lactate, 1 mM of diethyl pyrocarbonate (DEPC, a pharmacological inhibitor of the mitochondrial glutamate-aspartate antiporter) (Samartsev et al., 1997 ), 10 mM of N -acetyl- L -cysteine (NAC) or combinations thereof. All data represent means ± SEM. * P

    Journal: Frontiers in Pharmacology

    Article Title: Lactate does not activate NF-κB in oxidative tumor cells

    doi: 10.3389/fphar.2015.00228

    Figure Lengend Snippet: Inhibition of the malate-aspartate shuttle restores the ability of lactate to activate NF-κB in oxidative HeLa tumor cells . (A,B) NF-κB activity was determined using a dual luciferase reporter assay. HeLa ( A , N = 3; n = 12), SiHa ( B , N = 3; n = 12) cells were treated for the indicated amounts of time with vehicle (control), 10 mM of lactate, 1 mM of diethyl pyrocarbonate (DEPC, a pharmacological inhibitor of the mitochondrial glutamate-aspartate antiporter) (Samartsev et al., 1997 ), 10 mM of N -acetyl- L -cysteine (NAC) or combinations thereof. All data represent means ± SEM. * P

    Article Snippet: Where indicated, cells in fresh medium were treated with human TNFα (Miltenyi), sodium L -lactate (Sigma), N -acetyl-L -cysteine (NAC, Sigma), H2 O2 (Sigma), rotenone (Sigma) or diethyl pyrocarbonate (DEPC, Sigma).

    Techniques: Inhibition, Activity Assay, Luciferase, Reporter Assay

    Model showing that the oxidative use of NADH opposes NF-κB activation by lactate in oxidative tumor cells . In tumors, endothelial cells ( top ) and oxidative tumor cells ( bottom ) take up lactate, a process facilitated by the passive lactate-proton symporter monocarboxylate transporter 1 (MCT1). Both cell types convert lactate + NAD + to pyruvate + NADH + H + intracellularly (the lactate dehydrogenase B [LDHB] reaction). Pyruvate competes with α-ketoglutarate (αKG) to inhibit prolylhydroxylases (PHDs), resulting e.g., in the stabilization of hypoxia-inducible factor-1 subunit α (HIF-1α) (Vegran et al., 2011 ; De Saedeleer et al., 2012 ; Sonveaux et al., 2012 ). Endothelial cells do not use lactate-derived pyruvate and NADH as oxidative fuels (Sonveaux et al., 2012 ), thus further rendering NADH available to fuel NAD(P)H oxidases (Noxs) (Vegran et al., 2011 ). Together with pyruvate-mediated PHD inhibition, the production of reactive oxygen species (ROS) by Nox accounts for lactate-induced activation of nuclear factor-κB (NF-κB) in these cells. Comparatively, oxidative tumor cells use lactate (Sonveaux et al., 2008 ) and NADH (this study) as oxidative fuels. It renders these cells insensitive to lactate-induced NF-κB activation. NADH influx in mitochondria is controlled by the malate-aspartate shuttle gated by the oxoglutarate carrier (OGC, a malate-αKG exchanger) and by the glutamate-aspartate antiporter (GAA). Consequently, inhibiting the malate-aspartate shuttle can restore the ability of lactate to activate NF-κB in oxidative tumor cells, as illustrated in this study with HeLa cells treated by GAA inhibitor diethyl pyrocarbonate (DEPC). Other abbreviations: AST, aspartate aminotransferase; ETC, electron transport chain; IκBα, inhibitor and Cal27 of NF-κB α; Ikkα/β, inhibitor of NF-κB kinase α/β; MDH, malate dehydrogenase; MPC, mitochondrial pyruvate carrier; OXPHOS, oxidative phosphorylation.

    Journal: Frontiers in Pharmacology

    Article Title: Lactate does not activate NF-κB in oxidative tumor cells

    doi: 10.3389/fphar.2015.00228

    Figure Lengend Snippet: Model showing that the oxidative use of NADH opposes NF-κB activation by lactate in oxidative tumor cells . In tumors, endothelial cells ( top ) and oxidative tumor cells ( bottom ) take up lactate, a process facilitated by the passive lactate-proton symporter monocarboxylate transporter 1 (MCT1). Both cell types convert lactate + NAD + to pyruvate + NADH + H + intracellularly (the lactate dehydrogenase B [LDHB] reaction). Pyruvate competes with α-ketoglutarate (αKG) to inhibit prolylhydroxylases (PHDs), resulting e.g., in the stabilization of hypoxia-inducible factor-1 subunit α (HIF-1α) (Vegran et al., 2011 ; De Saedeleer et al., 2012 ; Sonveaux et al., 2012 ). Endothelial cells do not use lactate-derived pyruvate and NADH as oxidative fuels (Sonveaux et al., 2012 ), thus further rendering NADH available to fuel NAD(P)H oxidases (Noxs) (Vegran et al., 2011 ). Together with pyruvate-mediated PHD inhibition, the production of reactive oxygen species (ROS) by Nox accounts for lactate-induced activation of nuclear factor-κB (NF-κB) in these cells. Comparatively, oxidative tumor cells use lactate (Sonveaux et al., 2008 ) and NADH (this study) as oxidative fuels. It renders these cells insensitive to lactate-induced NF-κB activation. NADH influx in mitochondria is controlled by the malate-aspartate shuttle gated by the oxoglutarate carrier (OGC, a malate-αKG exchanger) and by the glutamate-aspartate antiporter (GAA). Consequently, inhibiting the malate-aspartate shuttle can restore the ability of lactate to activate NF-κB in oxidative tumor cells, as illustrated in this study with HeLa cells treated by GAA inhibitor diethyl pyrocarbonate (DEPC). Other abbreviations: AST, aspartate aminotransferase; ETC, electron transport chain; IκBα, inhibitor and Cal27 of NF-κB α; Ikkα/β, inhibitor of NF-κB kinase α/β; MDH, malate dehydrogenase; MPC, mitochondrial pyruvate carrier; OXPHOS, oxidative phosphorylation.

    Article Snippet: Where indicated, cells in fresh medium were treated with human TNFα (Miltenyi), sodium L -lactate (Sigma), N -acetyl-L -cysteine (NAC, Sigma), H2 O2 (Sigma), rotenone (Sigma) or diethyl pyrocarbonate (DEPC, Sigma).

    Techniques: Activation Assay, Derivative Assay, Inhibition, AST Assay