deoxyribonuclease i Thermo Fisher Search Results


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  • 99
    Millipore dnase i
    Dnase I, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 25071 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs dnasei
    Dnasei, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1367 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher deoxyribonuclease i dnase i
    Deoxyribonuclease I Dnase I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher deoxyribonuclease i
    Deoxyribonuclease I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1478 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher alexafluor488 dnase i
    Alexafluor488 Dnase I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 75/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher dnase i alexa 594
    A. F-/G-actin ratio in populations of cells subjected to 10% or 30% strain and stained after fixation at different times of stretching with phalloidin Alexa 647 for F-actin and <t>DNase-I</t> <t>Alexa</t> 594 for G-actin, measured as the ratio between total fluorescence intensities in the far red to red channels. (10% Strain, n = 2 independent experiments, 0min: 214 cells, 10min: 223 cells, 20 min: 246 cells, 35min: 248 cells. 30% Strain, n = 2 independent experiments, 0min: 310 cells, 10min: 331 cells, 20min: 280 cells, 45min: 175 cells). B. Mean value of the proportion of MRTF-A-GFP in the nucleus of cells and median value of the SiR-actin intensity relative to the initial fluorescence level as a function of time, in cells stretched by 10% vs control (10% strain: n = 3 independent experiments, 93 cells; control: n = 2 independent experiments, 124 cells). C. Same measurements for cells stretched by 30% vs control (30% strain: n = 4 independent experiments; 108 cells. Control n = 2, 53 cells). D. Confocal microscopy images of myoblasts in which F-actin was labelled with SiR-actin after fixation. Top: un-stretched control cell; top left: basal layer showing aligned actin stress fibers (z = 0); top right: apical actin with un-organized actin structure (z = 2.25 μm). Middle: cells after 5 min of 10% strain; middle left: basal layer with aligned actin stress fibers (z = 0), middle right: organized actin cap with aligned stress fibers above the nucleus (z = 2 μm). The contour of the nuclei was measured from the DAPI signal and drawn on the images. Bottom: cells after 5 min of 30% strain; bottom left: the cells have partly detached from the stretched substrate (z = 0), bottom right: organized actin cap with aligned stress fibers above the nucleus (z = 1.75 μm). E. Schematic view of a cell, showing the apical and basal levels that were imaged. F. Mean number of stress fibers above the nucleus rescaled by the size of the nucleus (width in μm in the direction perpendicular to the stress fibers). Control: 38 cells; 10% strain—t = 5 min: 66 cells, 10% strain—t = 15 min: 55 cells, 30% strain—t = 5min: 50 cells, 30% strain—t = 25 min: 41 cells.
    Dnase I Alexa 594, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 81/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher dnase i
    A. F-/G-actin ratio in populations of cells subjected to 10% or 30% strain and stained after fixation at different times of stretching with phalloidin Alexa 647 for F-actin and <t>DNase-I</t> <t>Alexa</t> 594 for G-actin, measured as the ratio between total fluorescence intensities in the far red to red channels. (10% Strain, n = 2 independent experiments, 0min: 214 cells, 10min: 223 cells, 20 min: 246 cells, 35min: 248 cells. 30% Strain, n = 2 independent experiments, 0min: 310 cells, 10min: 331 cells, 20min: 280 cells, 45min: 175 cells). B. Mean value of the proportion of MRTF-A-GFP in the nucleus of cells and median value of the SiR-actin intensity relative to the initial fluorescence level as a function of time, in cells stretched by 10% vs control (10% strain: n = 3 independent experiments, 93 cells; control: n = 2 independent experiments, 124 cells). C. Same measurements for cells stretched by 30% vs control (30% strain: n = 4 independent experiments; 108 cells. Control n = 2, 53 cells). D. Confocal microscopy images of myoblasts in which F-actin was labelled with SiR-actin after fixation. Top: un-stretched control cell; top left: basal layer showing aligned actin stress fibers (z = 0); top right: apical actin with un-organized actin structure (z = 2.25 μm). Middle: cells after 5 min of 10% strain; middle left: basal layer with aligned actin stress fibers (z = 0), middle right: organized actin cap with aligned stress fibers above the nucleus (z = 2 μm). The contour of the nuclei was measured from the DAPI signal and drawn on the images. Bottom: cells after 5 min of 30% strain; bottom left: the cells have partly detached from the stretched substrate (z = 0), bottom right: organized actin cap with aligned stress fibers above the nucleus (z = 1.75 μm). E. Schematic view of a cell, showing the apical and basal levels that were imaged. F. Mean number of stress fibers above the nucleus rescaled by the size of the nucleus (width in μm in the direction perpendicular to the stress fibers). Control: 38 cells; 10% strain—t = 5 min: 66 cells, 10% strain—t = 15 min: 55 cells, 30% strain—t = 5min: 50 cells, 30% strain—t = 25 min: 41 cells.
    Dnase I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 53370 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher deoxyribonuclease i enzyme
    A. F-/G-actin ratio in populations of cells subjected to 10% or 30% strain and stained after fixation at different times of stretching with phalloidin Alexa 647 for F-actin and <t>DNase-I</t> <t>Alexa</t> 594 for G-actin, measured as the ratio between total fluorescence intensities in the far red to red channels. (10% Strain, n = 2 independent experiments, 0min: 214 cells, 10min: 223 cells, 20 min: 246 cells, 35min: 248 cells. 30% Strain, n = 2 independent experiments, 0min: 310 cells, 10min: 331 cells, 20min: 280 cells, 45min: 175 cells). B. Mean value of the proportion of MRTF-A-GFP in the nucleus of cells and median value of the SiR-actin intensity relative to the initial fluorescence level as a function of time, in cells stretched by 10% vs control (10% strain: n = 3 independent experiments, 93 cells; control: n = 2 independent experiments, 124 cells). C. Same measurements for cells stretched by 30% vs control (30% strain: n = 4 independent experiments; 108 cells. Control n = 2, 53 cells). D. Confocal microscopy images of myoblasts in which F-actin was labelled with SiR-actin after fixation. Top: un-stretched control cell; top left: basal layer showing aligned actin stress fibers (z = 0); top right: apical actin with un-organized actin structure (z = 2.25 μm). Middle: cells after 5 min of 10% strain; middle left: basal layer with aligned actin stress fibers (z = 0), middle right: organized actin cap with aligned stress fibers above the nucleus (z = 2 μm). The contour of the nuclei was measured from the DAPI signal and drawn on the images. Bottom: cells after 5 min of 30% strain; bottom left: the cells have partly detached from the stretched substrate (z = 0), bottom right: organized actin cap with aligned stress fibers above the nucleus (z = 1.75 μm). E. Schematic view of a cell, showing the apical and basal levels that were imaged. F. Mean number of stress fibers above the nucleus rescaled by the size of the nucleus (width in μm in the direction perpendicular to the stress fibers). Control: 38 cells; 10% strain—t = 5 min: 66 cells, 10% strain—t = 15 min: 55 cells, 30% strain—t = 5min: 50 cells, 30% strain—t = 25 min: 41 cells.
    Deoxyribonuclease I Enzyme, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher deoxyribonuclease i dnase i rnase free reagent
    A. F-/G-actin ratio in populations of cells subjected to 10% or 30% strain and stained after fixation at different times of stretching with phalloidin Alexa 647 for F-actin and <t>DNase-I</t> <t>Alexa</t> 594 for G-actin, measured as the ratio between total fluorescence intensities in the far red to red channels. (10% Strain, n = 2 independent experiments, 0min: 214 cells, 10min: 223 cells, 20 min: 246 cells, 35min: 248 cells. 30% Strain, n = 2 independent experiments, 0min: 310 cells, 10min: 331 cells, 20min: 280 cells, 45min: 175 cells). B. Mean value of the proportion of MRTF-A-GFP in the nucleus of cells and median value of the SiR-actin intensity relative to the initial fluorescence level as a function of time, in cells stretched by 10% vs control (10% strain: n = 3 independent experiments, 93 cells; control: n = 2 independent experiments, 124 cells). C. Same measurements for cells stretched by 30% vs control (30% strain: n = 4 independent experiments; 108 cells. Control n = 2, 53 cells). D. Confocal microscopy images of myoblasts in which F-actin was labelled with SiR-actin after fixation. Top: un-stretched control cell; top left: basal layer showing aligned actin stress fibers (z = 0); top right: apical actin with un-organized actin structure (z = 2.25 μm). Middle: cells after 5 min of 10% strain; middle left: basal layer with aligned actin stress fibers (z = 0), middle right: organized actin cap with aligned stress fibers above the nucleus (z = 2 μm). The contour of the nuclei was measured from the DAPI signal and drawn on the images. Bottom: cells after 5 min of 30% strain; bottom left: the cells have partly detached from the stretched substrate (z = 0), bottom right: organized actin cap with aligned stress fibers above the nucleus (z = 1.75 μm). E. Schematic view of a cell, showing the apical and basal levels that were imaged. F. Mean number of stress fibers above the nucleus rescaled by the size of the nucleus (width in μm in the direction perpendicular to the stress fibers). Control: 38 cells; 10% strain—t = 5 min: 66 cells, 10% strain—t = 15 min: 55 cells, 30% strain—t = 5min: 50 cells, 30% strain—t = 25 min: 41 cells.
    Deoxyribonuclease I Dnase I Rnase Free Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 82/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher trypsin deoxyribonuclease i
    A. F-/G-actin ratio in populations of cells subjected to 10% or 30% strain and stained after fixation at different times of stretching with phalloidin Alexa 647 for F-actin and <t>DNase-I</t> <t>Alexa</t> 594 for G-actin, measured as the ratio between total fluorescence intensities in the far red to red channels. (10% Strain, n = 2 independent experiments, 0min: 214 cells, 10min: 223 cells, 20 min: 246 cells, 35min: 248 cells. 30% Strain, n = 2 independent experiments, 0min: 310 cells, 10min: 331 cells, 20min: 280 cells, 45min: 175 cells). B. Mean value of the proportion of MRTF-A-GFP in the nucleus of cells and median value of the SiR-actin intensity relative to the initial fluorescence level as a function of time, in cells stretched by 10% vs control (10% strain: n = 3 independent experiments, 93 cells; control: n = 2 independent experiments, 124 cells). C. Same measurements for cells stretched by 30% vs control (30% strain: n = 4 independent experiments; 108 cells. Control n = 2, 53 cells). D. Confocal microscopy images of myoblasts in which F-actin was labelled with SiR-actin after fixation. Top: un-stretched control cell; top left: basal layer showing aligned actin stress fibers (z = 0); top right: apical actin with un-organized actin structure (z = 2.25 μm). Middle: cells after 5 min of 10% strain; middle left: basal layer with aligned actin stress fibers (z = 0), middle right: organized actin cap with aligned stress fibers above the nucleus (z = 2 μm). The contour of the nuclei was measured from the DAPI signal and drawn on the images. Bottom: cells after 5 min of 30% strain; bottom left: the cells have partly detached from the stretched substrate (z = 0), bottom right: organized actin cap with aligned stress fibers above the nucleus (z = 1.75 μm). E. Schematic view of a cell, showing the apical and basal levels that were imaged. F. Mean number of stress fibers above the nucleus rescaled by the size of the nucleus (width in μm in the direction perpendicular to the stress fibers). Control: 38 cells; 10% strain—t = 5 min: 66 cells, 10% strain—t = 15 min: 55 cells, 30% strain—t = 5min: 50 cells, 30% strain—t = 25 min: 41 cells.
    Trypsin Deoxyribonuclease I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 70/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher dnase i buffer
    A. F-/G-actin ratio in populations of cells subjected to 10% or 30% strain and stained after fixation at different times of stretching with phalloidin Alexa 647 for F-actin and <t>DNase-I</t> <t>Alexa</t> 594 for G-actin, measured as the ratio between total fluorescence intensities in the far red to red channels. (10% Strain, n = 2 independent experiments, 0min: 214 cells, 10min: 223 cells, 20 min: 246 cells, 35min: 248 cells. 30% Strain, n = 2 independent experiments, 0min: 310 cells, 10min: 331 cells, 20min: 280 cells, 45min: 175 cells). B. Mean value of the proportion of MRTF-A-GFP in the nucleus of cells and median value of the SiR-actin intensity relative to the initial fluorescence level as a function of time, in cells stretched by 10% vs control (10% strain: n = 3 independent experiments, 93 cells; control: n = 2 independent experiments, 124 cells). C. Same measurements for cells stretched by 30% vs control (30% strain: n = 4 independent experiments; 108 cells. Control n = 2, 53 cells). D. Confocal microscopy images of myoblasts in which F-actin was labelled with SiR-actin after fixation. Top: un-stretched control cell; top left: basal layer showing aligned actin stress fibers (z = 0); top right: apical actin with un-organized actin structure (z = 2.25 μm). Middle: cells after 5 min of 10% strain; middle left: basal layer with aligned actin stress fibers (z = 0), middle right: organized actin cap with aligned stress fibers above the nucleus (z = 2 μm). The contour of the nuclei was measured from the DAPI signal and drawn on the images. Bottom: cells after 5 min of 30% strain; bottom left: the cells have partly detached from the stretched substrate (z = 0), bottom right: organized actin cap with aligned stress fibers above the nucleus (z = 1.75 μm). E. Schematic view of a cell, showing the apical and basal levels that were imaged. F. Mean number of stress fibers above the nucleus rescaled by the size of the nucleus (width in μm in the direction perpendicular to the stress fibers). Control: 38 cells; 10% strain—t = 5 min: 66 cells, 10% strain—t = 15 min: 55 cells, 30% strain—t = 5min: 50 cells, 30% strain—t = 25 min: 41 cells.
    Dnase I Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 424 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MACHEREY NAGEL dnase i digestion
    A. F-/G-actin ratio in populations of cells subjected to 10% or 30% strain and stained after fixation at different times of stretching with phalloidin Alexa 647 for F-actin and <t>DNase-I</t> <t>Alexa</t> 594 for G-actin, measured as the ratio between total fluorescence intensities in the far red to red channels. (10% Strain, n = 2 independent experiments, 0min: 214 cells, 10min: 223 cells, 20 min: 246 cells, 35min: 248 cells. 30% Strain, n = 2 independent experiments, 0min: 310 cells, 10min: 331 cells, 20min: 280 cells, 45min: 175 cells). B. Mean value of the proportion of MRTF-A-GFP in the nucleus of cells and median value of the SiR-actin intensity relative to the initial fluorescence level as a function of time, in cells stretched by 10% vs control (10% strain: n = 3 independent experiments, 93 cells; control: n = 2 independent experiments, 124 cells). C. Same measurements for cells stretched by 30% vs control (30% strain: n = 4 independent experiments; 108 cells. Control n = 2, 53 cells). D. Confocal microscopy images of myoblasts in which F-actin was labelled with SiR-actin after fixation. Top: un-stretched control cell; top left: basal layer showing aligned actin stress fibers (z = 0); top right: apical actin with un-organized actin structure (z = 2.25 μm). Middle: cells after 5 min of 10% strain; middle left: basal layer with aligned actin stress fibers (z = 0), middle right: organized actin cap with aligned stress fibers above the nucleus (z = 2 μm). The contour of the nuclei was measured from the DAPI signal and drawn on the images. Bottom: cells after 5 min of 30% strain; bottom left: the cells have partly detached from the stretched substrate (z = 0), bottom right: organized actin cap with aligned stress fibers above the nucleus (z = 1.75 μm). E. Schematic view of a cell, showing the apical and basal levels that were imaged. F. Mean number of stress fibers above the nucleus rescaled by the size of the nucleus (width in μm in the direction perpendicular to the stress fibers). Control: 38 cells; 10% strain—t = 5 min: 66 cells, 10% strain—t = 15 min: 55 cells, 30% strain—t = 5min: 50 cells, 30% strain—t = 25 min: 41 cells.
    Dnase I Digestion, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 89/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher dnase i stock
    A. F-/G-actin ratio in populations of cells subjected to 10% or 30% strain and stained after fixation at different times of stretching with phalloidin Alexa 647 for F-actin and <t>DNase-I</t> <t>Alexa</t> 594 for G-actin, measured as the ratio between total fluorescence intensities in the far red to red channels. (10% Strain, n = 2 independent experiments, 0min: 214 cells, 10min: 223 cells, 20 min: 246 cells, 35min: 248 cells. 30% Strain, n = 2 independent experiments, 0min: 310 cells, 10min: 331 cells, 20min: 280 cells, 45min: 175 cells). B. Mean value of the proportion of MRTF-A-GFP in the nucleus of cells and median value of the SiR-actin intensity relative to the initial fluorescence level as a function of time, in cells stretched by 10% vs control (10% strain: n = 3 independent experiments, 93 cells; control: n = 2 independent experiments, 124 cells). C. Same measurements for cells stretched by 30% vs control (30% strain: n = 4 independent experiments; 108 cells. Control n = 2, 53 cells). D. Confocal microscopy images of myoblasts in which F-actin was labelled with SiR-actin after fixation. Top: un-stretched control cell; top left: basal layer showing aligned actin stress fibers (z = 0); top right: apical actin with un-organized actin structure (z = 2.25 μm). Middle: cells after 5 min of 10% strain; middle left: basal layer with aligned actin stress fibers (z = 0), middle right: organized actin cap with aligned stress fibers above the nucleus (z = 2 μm). The contour of the nuclei was measured from the DAPI signal and drawn on the images. Bottom: cells after 5 min of 30% strain; bottom left: the cells have partly detached from the stretched substrate (z = 0), bottom right: organized actin cap with aligned stress fibers above the nucleus (z = 1.75 μm). E. Schematic view of a cell, showing the apical and basal levels that were imaged. F. Mean number of stress fibers above the nucleus rescaled by the size of the nucleus (width in μm in the direction perpendicular to the stress fibers). Control: 38 cells; 10% strain—t = 5 min: 66 cells, 10% strain—t = 15 min: 55 cells, 30% strain—t = 5min: 50 cells, 30% strain—t = 25 min: 41 cells.
    Dnase I Stock, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher turbo dnase i
    A. F-/G-actin ratio in populations of cells subjected to 10% or 30% strain and stained after fixation at different times of stretching with phalloidin Alexa 647 for F-actin and <t>DNase-I</t> <t>Alexa</t> 594 for G-actin, measured as the ratio between total fluorescence intensities in the far red to red channels. (10% Strain, n = 2 independent experiments, 0min: 214 cells, 10min: 223 cells, 20 min: 246 cells, 35min: 248 cells. 30% Strain, n = 2 independent experiments, 0min: 310 cells, 10min: 331 cells, 20min: 280 cells, 45min: 175 cells). B. Mean value of the proportion of MRTF-A-GFP in the nucleus of cells and median value of the SiR-actin intensity relative to the initial fluorescence level as a function of time, in cells stretched by 10% vs control (10% strain: n = 3 independent experiments, 93 cells; control: n = 2 independent experiments, 124 cells). C. Same measurements for cells stretched by 30% vs control (30% strain: n = 4 independent experiments; 108 cells. Control n = 2, 53 cells). D. Confocal microscopy images of myoblasts in which F-actin was labelled with SiR-actin after fixation. Top: un-stretched control cell; top left: basal layer showing aligned actin stress fibers (z = 0); top right: apical actin with un-organized actin structure (z = 2.25 μm). Middle: cells after 5 min of 10% strain; middle left: basal layer with aligned actin stress fibers (z = 0), middle right: organized actin cap with aligned stress fibers above the nucleus (z = 2 μm). The contour of the nuclei was measured from the DAPI signal and drawn on the images. Bottom: cells after 5 min of 30% strain; bottom left: the cells have partly detached from the stretched substrate (z = 0), bottom right: organized actin cap with aligned stress fibers above the nucleus (z = 1.75 μm). E. Schematic view of a cell, showing the apical and basal levels that were imaged. F. Mean number of stress fibers above the nucleus rescaled by the size of the nucleus (width in μm in the direction perpendicular to the stress fibers). Control: 38 cells; 10% strain—t = 5 min: 66 cells, 10% strain—t = 15 min: 55 cells, 30% strain—t = 5min: 50 cells, 30% strain—t = 25 min: 41 cells.
    Turbo Dnase I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1621 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher dnase i inhibitor
    A. F-/G-actin ratio in populations of cells subjected to 10% or 30% strain and stained after fixation at different times of stretching with phalloidin Alexa 647 for F-actin and <t>DNase-I</t> <t>Alexa</t> 594 for G-actin, measured as the ratio between total fluorescence intensities in the far red to red channels. (10% Strain, n = 2 independent experiments, 0min: 214 cells, 10min: 223 cells, 20 min: 246 cells, 35min: 248 cells. 30% Strain, n = 2 independent experiments, 0min: 310 cells, 10min: 331 cells, 20min: 280 cells, 45min: 175 cells). B. Mean value of the proportion of MRTF-A-GFP in the nucleus of cells and median value of the SiR-actin intensity relative to the initial fluorescence level as a function of time, in cells stretched by 10% vs control (10% strain: n = 3 independent experiments, 93 cells; control: n = 2 independent experiments, 124 cells). C. Same measurements for cells stretched by 30% vs control (30% strain: n = 4 independent experiments; 108 cells. Control n = 2, 53 cells). D. Confocal microscopy images of myoblasts in which F-actin was labelled with SiR-actin after fixation. Top: un-stretched control cell; top left: basal layer showing aligned actin stress fibers (z = 0); top right: apical actin with un-organized actin structure (z = 2.25 μm). Middle: cells after 5 min of 10% strain; middle left: basal layer with aligned actin stress fibers (z = 0), middle right: organized actin cap with aligned stress fibers above the nucleus (z = 2 μm). The contour of the nuclei was measured from the DAPI signal and drawn on the images. Bottom: cells after 5 min of 30% strain; bottom left: the cells have partly detached from the stretched substrate (z = 0), bottom right: organized actin cap with aligned stress fibers above the nucleus (z = 1.75 μm). E. Schematic view of a cell, showing the apical and basal levels that were imaged. F. Mean number of stress fibers above the nucleus rescaled by the size of the nucleus (width in μm in the direction perpendicular to the stress fibers). Control: 38 cells; 10% strain—t = 5 min: 66 cells, 10% strain—t = 15 min: 55 cells, 30% strain—t = 5min: 50 cells, 30% strain—t = 25 min: 41 cells.
    Dnase I Inhibitor, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    A. F-/G-actin ratio in populations of cells subjected to 10% or 30% strain and stained after fixation at different times of stretching with phalloidin Alexa 647 for F-actin and <t>DNase-I</t> <t>Alexa</t> 594 for G-actin, measured as the ratio between total fluorescence intensities in the far red to red channels. (10% Strain, n = 2 independent experiments, 0min: 214 cells, 10min: 223 cells, 20 min: 246 cells, 35min: 248 cells. 30% Strain, n = 2 independent experiments, 0min: 310 cells, 10min: 331 cells, 20min: 280 cells, 45min: 175 cells). B. Mean value of the proportion of MRTF-A-GFP in the nucleus of cells and median value of the SiR-actin intensity relative to the initial fluorescence level as a function of time, in cells stretched by 10% vs control (10% strain: n = 3 independent experiments, 93 cells; control: n = 2 independent experiments, 124 cells). C. Same measurements for cells stretched by 30% vs control (30% strain: n = 4 independent experiments; 108 cells. Control n = 2, 53 cells). D. Confocal microscopy images of myoblasts in which F-actin was labelled with SiR-actin after fixation. Top: un-stretched control cell; top left: basal layer showing aligned actin stress fibers (z = 0); top right: apical actin with un-organized actin structure (z = 2.25 μm). Middle: cells after 5 min of 10% strain; middle left: basal layer with aligned actin stress fibers (z = 0), middle right: organized actin cap with aligned stress fibers above the nucleus (z = 2 μm). The contour of the nuclei was measured from the DAPI signal and drawn on the images. Bottom: cells after 5 min of 30% strain; bottom left: the cells have partly detached from the stretched substrate (z = 0), bottom right: organized actin cap with aligned stress fibers above the nucleus (z = 1.75 μm). E. Schematic view of a cell, showing the apical and basal levels that were imaged. F. Mean number of stress fibers above the nucleus rescaled by the size of the nucleus (width in μm in the direction perpendicular to the stress fibers). Control: 38 cells; 10% strain—t = 5 min: 66 cells, 10% strain—t = 15 min: 55 cells, 30% strain—t = 5min: 50 cells, 30% strain—t = 25 min: 41 cells.
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    The spatial distribution of actin in RBC ghosts revealed by fluorescent probes of phalloidin-rhodamine and DNase <t>I–FITC.</t> The spatial distribution of the fluorescent probes was analyzed via a three-dimensional optical sectioning by a confocal inverted laser scanning microscope. ( A ) Optical sections ( a–h , 0.6 μm apart) of RBC ghost labeled by phalloidin-rhodamine (4.4 μM). Each square of the gallery of images possesses a dimension of 9.5 μm × 9.5 μm. ( B ) The distribution of phalloidin-rhodamine and DNase I–FITC in RBC ghosts in an optical section taken at the middle (half thickness) of the RBC ghost. Inset , The separate distribution of phalloidin-rhodamine ( red ) and <t>DNase</t> I ( green ) in a RBC ghost that was labeled with both phalloidin-rhodamine and DNase I–FITC. Bars: ( A ) 5 μm; ( B ) 10 μm.
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    The spatial distribution of actin in RBC ghosts revealed by fluorescent probes of phalloidin-rhodamine and DNase <t>I–FITC.</t> The spatial distribution of the fluorescent probes was analyzed via a three-dimensional optical sectioning by a confocal inverted laser scanning microscope. ( A ) Optical sections ( a–h , 0.6 μm apart) of RBC ghost labeled by phalloidin-rhodamine (4.4 μM). Each square of the gallery of images possesses a dimension of 9.5 μm × 9.5 μm. ( B ) The distribution of phalloidin-rhodamine and DNase I–FITC in RBC ghosts in an optical section taken at the middle (half thickness) of the RBC ghost. Inset , The separate distribution of phalloidin-rhodamine ( red ) and <t>DNase</t> I ( green ) in a RBC ghost that was labeled with both phalloidin-rhodamine and DNase I–FITC. Bars: ( A ) 5 μm; ( B ) 10 μm.
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    Image Search Results


    A. F-/G-actin ratio in populations of cells subjected to 10% or 30% strain and stained after fixation at different times of stretching with phalloidin Alexa 647 for F-actin and DNase-I Alexa 594 for G-actin, measured as the ratio between total fluorescence intensities in the far red to red channels. (10% Strain, n = 2 independent experiments, 0min: 214 cells, 10min: 223 cells, 20 min: 246 cells, 35min: 248 cells. 30% Strain, n = 2 independent experiments, 0min: 310 cells, 10min: 331 cells, 20min: 280 cells, 45min: 175 cells). B. Mean value of the proportion of MRTF-A-GFP in the nucleus of cells and median value of the SiR-actin intensity relative to the initial fluorescence level as a function of time, in cells stretched by 10% vs control (10% strain: n = 3 independent experiments, 93 cells; control: n = 2 independent experiments, 124 cells). C. Same measurements for cells stretched by 30% vs control (30% strain: n = 4 independent experiments; 108 cells. Control n = 2, 53 cells). D. Confocal microscopy images of myoblasts in which F-actin was labelled with SiR-actin after fixation. Top: un-stretched control cell; top left: basal layer showing aligned actin stress fibers (z = 0); top right: apical actin with un-organized actin structure (z = 2.25 μm). Middle: cells after 5 min of 10% strain; middle left: basal layer with aligned actin stress fibers (z = 0), middle right: organized actin cap with aligned stress fibers above the nucleus (z = 2 μm). The contour of the nuclei was measured from the DAPI signal and drawn on the images. Bottom: cells after 5 min of 30% strain; bottom left: the cells have partly detached from the stretched substrate (z = 0), bottom right: organized actin cap with aligned stress fibers above the nucleus (z = 1.75 μm). E. Schematic view of a cell, showing the apical and basal levels that were imaged. F. Mean number of stress fibers above the nucleus rescaled by the size of the nucleus (width in μm in the direction perpendicular to the stress fibers). Control: 38 cells; 10% strain—t = 5 min: 66 cells, 10% strain—t = 15 min: 55 cells, 30% strain—t = 5min: 50 cells, 30% strain—t = 25 min: 41 cells.

    Journal: PLoS ONE

    Article Title: The nature and intensity of mechanical stimulation drive different dynamics of MRTF-A nuclear redistribution after actin remodeling in myoblasts

    doi: 10.1371/journal.pone.0214385

    Figure Lengend Snippet: A. F-/G-actin ratio in populations of cells subjected to 10% or 30% strain and stained after fixation at different times of stretching with phalloidin Alexa 647 for F-actin and DNase-I Alexa 594 for G-actin, measured as the ratio between total fluorescence intensities in the far red to red channels. (10% Strain, n = 2 independent experiments, 0min: 214 cells, 10min: 223 cells, 20 min: 246 cells, 35min: 248 cells. 30% Strain, n = 2 independent experiments, 0min: 310 cells, 10min: 331 cells, 20min: 280 cells, 45min: 175 cells). B. Mean value of the proportion of MRTF-A-GFP in the nucleus of cells and median value of the SiR-actin intensity relative to the initial fluorescence level as a function of time, in cells stretched by 10% vs control (10% strain: n = 3 independent experiments, 93 cells; control: n = 2 independent experiments, 124 cells). C. Same measurements for cells stretched by 30% vs control (30% strain: n = 4 independent experiments; 108 cells. Control n = 2, 53 cells). D. Confocal microscopy images of myoblasts in which F-actin was labelled with SiR-actin after fixation. Top: un-stretched control cell; top left: basal layer showing aligned actin stress fibers (z = 0); top right: apical actin with un-organized actin structure (z = 2.25 μm). Middle: cells after 5 min of 10% strain; middle left: basal layer with aligned actin stress fibers (z = 0), middle right: organized actin cap with aligned stress fibers above the nucleus (z = 2 μm). The contour of the nuclei was measured from the DAPI signal and drawn on the images. Bottom: cells after 5 min of 30% strain; bottom left: the cells have partly detached from the stretched substrate (z = 0), bottom right: organized actin cap with aligned stress fibers above the nucleus (z = 1.75 μm). E. Schematic view of a cell, showing the apical and basal levels that were imaged. F. Mean number of stress fibers above the nucleus rescaled by the size of the nucleus (width in μm in the direction perpendicular to the stress fibers). Control: 38 cells; 10% strain—t = 5 min: 66 cells, 10% strain—t = 15 min: 55 cells, 30% strain—t = 5min: 50 cells, 30% strain—t = 25 min: 41 cells.

    Article Snippet: Immunostaining Cells were fixed in a 4% paraformaldehyde solution for 20 min and stained with phalloidin Alexa Fluor 647 or 488 at 0.026 nmol/l, DNase-I Alexa 594 at 0.16 nmol/l and DAPI at 1 μg/ml (all from Life Technologies) for 30 min at room temperature or overnight at 4°C, after permeabilization with 0.5% Triton X-100 in PBS and saturation.

    Techniques: Staining, Fluorescence, Confocal Microscopy

    Effect of calpain-like silencing during PCD. (A) DNA fragmentation in calpain-like silenced trophozoites after PCD induction by G418. Confocal microscopy analysis of trophozoites showing the TUNEL staining, samples were counter-stained with DAPI. (A) Negative control, untreated trophozoites; (B) Positive control, trophozoites treated with 20 μg of DNase I endonuclease for 30 min; (C) Trophozoites after a 9-h incubation with 10 μg/ml of G418; (D,E) Trophozoites pre-incubated for 24 h with NRS (D) or calpain-like (E) siRNAs sequences and then 9 h with 10 μg/ml G418; (B) Densitometric analysis of (A). (C) Effect of calpain-like silencing on the cell viability of E. histolytica incubated with G418; (A) Negative control, untreated trophozoites; (B) Trophozoites after a 9-h incubation with 10 μg/ml of G418; (C,D) Trophozoites pre-incubated for 24 h with NRS (C) or calpain-like (D) siRNAs sequences and then 9 h with 10 μg/ml G418. *indicate statistically significant difference: P

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: A Calpain-Like Protein Is Involved in the Execution Phase of Programmed Cell Death of Entamoeba histolytica

    doi: 10.3389/fcimb.2018.00339

    Figure Lengend Snippet: Effect of calpain-like silencing during PCD. (A) DNA fragmentation in calpain-like silenced trophozoites after PCD induction by G418. Confocal microscopy analysis of trophozoites showing the TUNEL staining, samples were counter-stained with DAPI. (A) Negative control, untreated trophozoites; (B) Positive control, trophozoites treated with 20 μg of DNase I endonuclease for 30 min; (C) Trophozoites after a 9-h incubation with 10 μg/ml of G418; (D,E) Trophozoites pre-incubated for 24 h with NRS (D) or calpain-like (E) siRNAs sequences and then 9 h with 10 μg/ml G418; (B) Densitometric analysis of (A). (C) Effect of calpain-like silencing on the cell viability of E. histolytica incubated with G418; (A) Negative control, untreated trophozoites; (B) Trophozoites after a 9-h incubation with 10 μg/ml of G418; (C,D) Trophozoites pre-incubated for 24 h with NRS (C) or calpain-like (D) siRNAs sequences and then 9 h with 10 μg/ml G418. *indicate statistically significant difference: P

    Article Snippet: As a positive control, trophozoites were treated with 20 mg/ml DNase I endonuclease (Invitrogen) for 30 min, and non-treated trophozoites were used as a negative control.

    Techniques: Confocal Microscopy, TUNEL Assay, Staining, Negative Control, Positive Control, Incubation

    Enzymatic resistance of phosphorothioate-modified Au-TDNNs. (A, B) Fluorescence time graph depicting terminal-modified Au-TDNN and overall-modified Au-TDNN degradation by DNase I. (C, D) Fluorescence time graph depicting terminal-modified Au-TDNN and overall-modified Au-TDNN degradation by Exo III.

    Journal: Theranostics

    Article Title: High-Discrimination Factor Nanosensor Based on Tetrahedral DNA Nanostructures and Gold Nanoparticles for Detection of MiRNA-21 in Live Cells

    doi: 10.7150/thno.23852

    Figure Lengend Snippet: Enzymatic resistance of phosphorothioate-modified Au-TDNNs. (A, B) Fluorescence time graph depicting terminal-modified Au-TDNN and overall-modified Au-TDNN degradation by DNase I. (C, D) Fluorescence time graph depicting terminal-modified Au-TDNN and overall-modified Au-TDNN degradation by Exo III.

    Article Snippet: For both human DNase I (Thermo Scientific) and exonuclease III (New England Biolabs) digestion, a common concentration of 3 U/mL was used to digest 2 nM Au-TDNNs samples in PBS.

    Techniques: Modification, Fluorescence

    The spatial distribution of actin in RBC ghosts revealed by fluorescent probes of phalloidin-rhodamine and DNase I–FITC. The spatial distribution of the fluorescent probes was analyzed via a three-dimensional optical sectioning by a confocal inverted laser scanning microscope. ( A ) Optical sections ( a–h , 0.6 μm apart) of RBC ghost labeled by phalloidin-rhodamine (4.4 μM). Each square of the gallery of images possesses a dimension of 9.5 μm × 9.5 μm. ( B ) The distribution of phalloidin-rhodamine and DNase I–FITC in RBC ghosts in an optical section taken at the middle (half thickness) of the RBC ghost. Inset , The separate distribution of phalloidin-rhodamine ( red ) and DNase I ( green ) in a RBC ghost that was labeled with both phalloidin-rhodamine and DNase I–FITC. Bars: ( A ) 5 μm; ( B ) 10 μm.

    Journal: The Journal of Cell Biology

    Article Title: Mechanical Fluctuations of the Membrane-Skeleton Are Dependent on F-Actin ATPase in Human Erythrocytes

    doi:

    Figure Lengend Snippet: The spatial distribution of actin in RBC ghosts revealed by fluorescent probes of phalloidin-rhodamine and DNase I–FITC. The spatial distribution of the fluorescent probes was analyzed via a three-dimensional optical sectioning by a confocal inverted laser scanning microscope. ( A ) Optical sections ( a–h , 0.6 μm apart) of RBC ghost labeled by phalloidin-rhodamine (4.4 μM). Each square of the gallery of images possesses a dimension of 9.5 μm × 9.5 μm. ( B ) The distribution of phalloidin-rhodamine and DNase I–FITC in RBC ghosts in an optical section taken at the middle (half thickness) of the RBC ghost. Inset , The separate distribution of phalloidin-rhodamine ( red ) and DNase I ( green ) in a RBC ghost that was labeled with both phalloidin-rhodamine and DNase I–FITC. Bars: ( A ) 5 μm; ( B ) 10 μm.

    Article Snippet: The RBC ghosts were washed twice with a KCl solution, and then incubated with 4.4 μM phalloidin-rhodamine (Molecular Probes, Inc., Eugene, OR) alone or with a combination of 4.4 μM phalloidin-rhodamine and 35 nM DNase I–FITC (Molecular Probes, Inc.).

    Techniques: Laser-Scanning Microscopy, Labeling