dbco s s peg3 biotin Search Results


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  • 99
    Qiagen qiaquick nucleotide removal kit
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    Click Chemistry Tools dbco s s peg3 biotin conjugate
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    Click Chemistry Tools dbco s s peg3 biotin
    (a) Schematic for the enrichment of O -GlcNAc peptides with chemoenzymatic labeling, copper-free click chemistry, and reductant-cleavable <t>DBCO–S–S–PEG3–biotin.</t> (b,c) The structures of Reagent 1 (i.e., DBCO–S–S–PEG3–biotin) and Reagent 2 (i.e., APTA).
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    Click Chemistry Tools disulfide biotin linker
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    Thermo Fisher phosphine peg3 biotin
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    Thermo Fisher phosphate buffered saline pbs
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    Millipore magnesium chloride
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    Qiagen minelute pcr purification kit
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    Millipore trizma base tris
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    Fisher Scientific dimethyl sulfoxide dmso
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    Qiagen qiaquick pcr purification kit
    (a) Schematic for the enrichment of O -GlcNAc peptides with chemoenzymatic labeling, copper-free click chemistry, and reductant-cleavable <t>DBCO–S–S–PEG3–biotin.</t> (b,c) The structures of Reagent 1 (i.e., DBCO–S–S–PEG3–biotin) and Reagent 2 (i.e., APTA).
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    KeraFAST dbco cy5
    Replacing the C1–OAc of Ac 4 ManAz with a cleavable ether bond–enabled controlled metabolic cell labeling in vitro and in vivo ( a ) Synthetic route of dormant Ac 3 ManAz derivatives including Ac 3 ManAzEt and UV-responsive Ac 3 ManAzNB. ( b ) Schematic illustration of UV-irradiation-activated metabolic labeling of Ac 3 ManAzNB and subsequent detection of the expressed azido groups by <t>DBCO–Cy5</t> via click chemistry. ( c ) Confocal laser scanning microscopy (CLSM) images of LS174T colon cancer cells after incubation with Ac 4 ManAz (50 μM), Ac 3 ManAzEt (50 μM), Ac 3 ManAzNB (50 μM) − UV, Ac 3 ManAzNB (50 μM) + UV or PBS for 72 h and labeling with DBCO–Cy5 (50 μM, red) for 1 h. The cell nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI, blue). Scale bars, 10 μm. ( d , e ) Ac 4 ManAz, Ac 3 ManAzEt or Ac 3 ManAzNB (25 mM, 20 μl) was injected intratumorally (i.t.) to the left LS174T tumors of athymic nude mice once daily for three consecutive days (days 1–3), and the right LS174T tumors were injected i.t. with PBS as controls. DBCO–Cy5 (5 mg/kg) was i.v. injected on day 4. ( d ) In vivo fluorescence imaging of mice from different groups at 48 h p.i. of DBCO–Cy5. Tumors are shown by yellow arrows. ( e ) Ex vivo Cy5 fluorescence intensity of the tumor tissues harvested at 48 h p.i. of DBCO–Cy5. Data are presented as mean ± s.e.m. ( n = 3) and analyzed by one-way ANOV A (Fisher; 0.01
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    Click Chemistry Tools dibenzylcyclooctyne cy5 5
    Replacing the C1–OAc of Ac 4 ManAz with a cleavable ether bond–enabled controlled metabolic cell labeling in vitro and in vivo ( a ) Synthetic route of dormant Ac 3 ManAz derivatives including Ac 3 ManAzEt and UV-responsive Ac 3 ManAzNB. ( b ) Schematic illustration of UV-irradiation-activated metabolic labeling of Ac 3 ManAzNB and subsequent detection of the expressed azido groups by <t>DBCO–Cy5</t> via click chemistry. ( c ) Confocal laser scanning microscopy (CLSM) images of LS174T colon cancer cells after incubation with Ac 4 ManAz (50 μM), Ac 3 ManAzEt (50 μM), Ac 3 ManAzNB (50 μM) − UV, Ac 3 ManAzNB (50 μM) + UV or PBS for 72 h and labeling with DBCO–Cy5 (50 μM, red) for 1 h. The cell nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI, blue). Scale bars, 10 μm. ( d , e ) Ac 4 ManAz, Ac 3 ManAzEt or Ac 3 ManAzNB (25 mM, 20 μl) was injected intratumorally (i.t.) to the left LS174T tumors of athymic nude mice once daily for three consecutive days (days 1–3), and the right LS174T tumors were injected i.t. with PBS as controls. DBCO–Cy5 (5 mg/kg) was i.v. injected on day 4. ( d ) In vivo fluorescence imaging of mice from different groups at 48 h p.i. of DBCO–Cy5. Tumors are shown by yellow arrows. ( e ) Ex vivo Cy5 fluorescence intensity of the tumor tissues harvested at 48 h p.i. of DBCO–Cy5. Data are presented as mean ± s.e.m. ( n = 3) and analyzed by one-way ANOV A (Fisher; 0.01
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    Thermo Fisher glycogen
    Replacing the C1–OAc of Ac 4 ManAz with a cleavable ether bond–enabled controlled metabolic cell labeling in vitro and in vivo ( a ) Synthetic route of dormant Ac 3 ManAz derivatives including Ac 3 ManAzEt and UV-responsive Ac 3 ManAzNB. ( b ) Schematic illustration of UV-irradiation-activated metabolic labeling of Ac 3 ManAzNB and subsequent detection of the expressed azido groups by <t>DBCO–Cy5</t> via click chemistry. ( c ) Confocal laser scanning microscopy (CLSM) images of LS174T colon cancer cells after incubation with Ac 4 ManAz (50 μM), Ac 3 ManAzEt (50 μM), Ac 3 ManAzNB (50 μM) − UV, Ac 3 ManAzNB (50 μM) + UV or PBS for 72 h and labeling with DBCO–Cy5 (50 μM, red) for 1 h. The cell nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI, blue). Scale bars, 10 μm. ( d , e ) Ac 4 ManAz, Ac 3 ManAzEt or Ac 3 ManAzNB (25 mM, 20 μl) was injected intratumorally (i.t.) to the left LS174T tumors of athymic nude mice once daily for three consecutive days (days 1–3), and the right LS174T tumors were injected i.t. with PBS as controls. DBCO–Cy5 (5 mg/kg) was i.v. injected on day 4. ( d ) In vivo fluorescence imaging of mice from different groups at 48 h p.i. of DBCO–Cy5. Tumors are shown by yellow arrows. ( e ) Ex vivo Cy5 fluorescence intensity of the tumor tissues harvested at 48 h p.i. of DBCO–Cy5. Data are presented as mean ± s.e.m. ( n = 3) and analyzed by one-way ANOV A (Fisher; 0.01
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    Eppendorf AG thermomixer
    Replacing the C1–OAc of Ac 4 ManAz with a cleavable ether bond–enabled controlled metabolic cell labeling in vitro and in vivo ( a ) Synthetic route of dormant Ac 3 ManAz derivatives including Ac 3 ManAzEt and UV-responsive Ac 3 ManAzNB. ( b ) Schematic illustration of UV-irradiation-activated metabolic labeling of Ac 3 ManAzNB and subsequent detection of the expressed azido groups by <t>DBCO–Cy5</t> via click chemistry. ( c ) Confocal laser scanning microscopy (CLSM) images of LS174T colon cancer cells after incubation with Ac 4 ManAz (50 μM), Ac 3 ManAzEt (50 μM), Ac 3 ManAzNB (50 μM) − UV, Ac 3 ManAzNB (50 μM) + UV or PBS for 72 h and labeling with DBCO–Cy5 (50 μM, red) for 1 h. The cell nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI, blue). Scale bars, 10 μm. ( d , e ) Ac 4 ManAz, Ac 3 ManAzEt or Ac 3 ManAzNB (25 mM, 20 μl) was injected intratumorally (i.t.) to the left LS174T tumors of athymic nude mice once daily for three consecutive days (days 1–3), and the right LS174T tumors were injected i.t. with PBS as controls. DBCO–Cy5 (5 mg/kg) was i.v. injected on day 4. ( d ) In vivo fluorescence imaging of mice from different groups at 48 h p.i. of DBCO–Cy5. Tumors are shown by yellow arrows. ( e ) Ex vivo Cy5 fluorescence intensity of the tumor tissues harvested at 48 h p.i. of DBCO–Cy5. Data are presented as mean ± s.e.m. ( n = 3) and analyzed by one-way ANOV A (Fisher; 0.01
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    Image Search Results


    (a) Schematic for the enrichment of O -GlcNAc peptides with chemoenzymatic labeling, copper-free click chemistry, and reductant-cleavable DBCO–S–S–PEG3–biotin. (b,c) The structures of Reagent 1 (i.e., DBCO–S–S–PEG3–biotin) and Reagent 2 (i.e., APTA).

    Journal: Analytical chemistry

    Article Title: O‑GlcNAc Site Mapping by Using a Combination of Chemoenzymatic Labeling, Copper-Free Click Chemistry, Reductive Cleavage, and Electron-Transfer Dissociation Mass Spectrometry

    doi: 10.1021/acs.analchem.8b05688

    Figure Lengend Snippet: (a) Schematic for the enrichment of O -GlcNAc peptides with chemoenzymatic labeling, copper-free click chemistry, and reductant-cleavable DBCO–S–S–PEG3–biotin. (b,c) The structures of Reagent 1 (i.e., DBCO–S–S–PEG3–biotin) and Reagent 2 (i.e., APTA).

    Article Snippet: Copper-free click chemistry was performed in 15 μ L of PBS and 4 μ L of 1 mM DBCO–S–S–PEG3–biotin (predissolved in DMSO; Click Chemistry Tools LLC) for 2 h at room temperature.

    Techniques: Labeling

    Replacing the C1–OAc of Ac 4 ManAz with a cleavable ether bond–enabled controlled metabolic cell labeling in vitro and in vivo ( a ) Synthetic route of dormant Ac 3 ManAz derivatives including Ac 3 ManAzEt and UV-responsive Ac 3 ManAzNB. ( b ) Schematic illustration of UV-irradiation-activated metabolic labeling of Ac 3 ManAzNB and subsequent detection of the expressed azido groups by DBCO–Cy5 via click chemistry. ( c ) Confocal laser scanning microscopy (CLSM) images of LS174T colon cancer cells after incubation with Ac 4 ManAz (50 μM), Ac 3 ManAzEt (50 μM), Ac 3 ManAzNB (50 μM) − UV, Ac 3 ManAzNB (50 μM) + UV or PBS for 72 h and labeling with DBCO–Cy5 (50 μM, red) for 1 h. The cell nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI, blue). Scale bars, 10 μm. ( d , e ) Ac 4 ManAz, Ac 3 ManAzEt or Ac 3 ManAzNB (25 mM, 20 μl) was injected intratumorally (i.t.) to the left LS174T tumors of athymic nude mice once daily for three consecutive days (days 1–3), and the right LS174T tumors were injected i.t. with PBS as controls. DBCO–Cy5 (5 mg/kg) was i.v. injected on day 4. ( d ) In vivo fluorescence imaging of mice from different groups at 48 h p.i. of DBCO–Cy5. Tumors are shown by yellow arrows. ( e ) Ex vivo Cy5 fluorescence intensity of the tumor tissues harvested at 48 h p.i. of DBCO–Cy5. Data are presented as mean ± s.e.m. ( n = 3) and analyzed by one-way ANOV A (Fisher; 0.01

    Journal: Nature chemical biology

    Article Title: Selective in vivo metabolic cell-labeling-mediated cancer targeting

    doi: 10.1038/nchembio.2297

    Figure Lengend Snippet: Replacing the C1–OAc of Ac 4 ManAz with a cleavable ether bond–enabled controlled metabolic cell labeling in vitro and in vivo ( a ) Synthetic route of dormant Ac 3 ManAz derivatives including Ac 3 ManAzEt and UV-responsive Ac 3 ManAzNB. ( b ) Schematic illustration of UV-irradiation-activated metabolic labeling of Ac 3 ManAzNB and subsequent detection of the expressed azido groups by DBCO–Cy5 via click chemistry. ( c ) Confocal laser scanning microscopy (CLSM) images of LS174T colon cancer cells after incubation with Ac 4 ManAz (50 μM), Ac 3 ManAzEt (50 μM), Ac 3 ManAzNB (50 μM) − UV, Ac 3 ManAzNB (50 μM) + UV or PBS for 72 h and labeling with DBCO–Cy5 (50 μM, red) for 1 h. The cell nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI, blue). Scale bars, 10 μm. ( d , e ) Ac 4 ManAz, Ac 3 ManAzEt or Ac 3 ManAzNB (25 mM, 20 μl) was injected intratumorally (i.t.) to the left LS174T tumors of athymic nude mice once daily for three consecutive days (days 1–3), and the right LS174T tumors were injected i.t. with PBS as controls. DBCO–Cy5 (5 mg/kg) was i.v. injected on day 4. ( d ) In vivo fluorescence imaging of mice from different groups at 48 h p.i. of DBCO–Cy5. Tumors are shown by yellow arrows. ( e ) Ex vivo Cy5 fluorescence intensity of the tumor tissues harvested at 48 h p.i. of DBCO–Cy5. Data are presented as mean ± s.e.m. ( n = 3) and analyzed by one-way ANOV A (Fisher; 0.01

    Article Snippet: DBCO–Cy3 and DBCO–Cy5 were purchased from KeraFAST, Inc (Boston, MA, USA).

    Techniques: Labeling, In Vitro, In Vivo, Irradiation, Confocal Laser Scanning Microscopy, Incubation, Staining, Injection, Mouse Assay, Fluorescence, Imaging, Ex Vivo

    DCL-AAM-mediated cancer-selective labeling in vitro ( a ) Schematic illustration of HDAC/CT SL-induced degradation of DCL-AAM. HDAC removes the N 6 -acetyl group of the lysine residue first, which is followed by cleavage of the C 1 -amide bond by CT SL and self-cleavage of (4-aminophenyl) (phenyl)methanol (PL) to release Ac 3 ManAzOH. ( b , c ) CLSM images of LS174T and 4T1 cells ( b ) and MCF-10A breast basal epithelial cells ( c ) after incubation with DCL-AAM (50 μM), DCL-AAM (50 μM) + TSA (1 μM), DCL-AAM (50 μM) + Z-FY-CHO (50 μM), or PBS for 72 h and treatment with DBCO–Cy5 (50 μM, red) for 1 h. The cell nuclei were stained with DAPI (blue). Scale bars, 10 μm. ( d ) Average Cy5 fluorescence intensity of LS174T, 4T1 and MCF-10A cells following the same treatment as described in b and c . Data are presented as mean ± s.e.m. ( n = 6) and analyzed by one-way ANOV A (Fisher; 0.01

    Journal: Nature chemical biology

    Article Title: Selective in vivo metabolic cell-labeling-mediated cancer targeting

    doi: 10.1038/nchembio.2297

    Figure Lengend Snippet: DCL-AAM-mediated cancer-selective labeling in vitro ( a ) Schematic illustration of HDAC/CT SL-induced degradation of DCL-AAM. HDAC removes the N 6 -acetyl group of the lysine residue first, which is followed by cleavage of the C 1 -amide bond by CT SL and self-cleavage of (4-aminophenyl) (phenyl)methanol (PL) to release Ac 3 ManAzOH. ( b , c ) CLSM images of LS174T and 4T1 cells ( b ) and MCF-10A breast basal epithelial cells ( c ) after incubation with DCL-AAM (50 μM), DCL-AAM (50 μM) + TSA (1 μM), DCL-AAM (50 μM) + Z-FY-CHO (50 μM), or PBS for 72 h and treatment with DBCO–Cy5 (50 μM, red) for 1 h. The cell nuclei were stained with DAPI (blue). Scale bars, 10 μm. ( d ) Average Cy5 fluorescence intensity of LS174T, 4T1 and MCF-10A cells following the same treatment as described in b and c . Data are presented as mean ± s.e.m. ( n = 6) and analyzed by one-way ANOV A (Fisher; 0.01

    Article Snippet: DBCO–Cy3 and DBCO–Cy5 were purchased from KeraFAST, Inc (Boston, MA, USA).

    Techniques: Labeling, In Vitro, Confocal Laser Scanning Microscopy, Incubation, Staining, Fluorescence

    DCL-AAM mediated cancer-selective labeling in vivo ( a–c ) DCL-AAM (60 mg/kg), Ac 4 ManAz (40 mg/kg) or PB S was i.v. injected into athymic nude mice bearing subcutaneous LS174T tumors once daily for 3 d. At 24 h after the last injection, the metabolic expression of azido groups in tumor tissues was analyzed by western blotting ( a ) or by monitoring the biodistribution of i.v.-injected DBCO–Cy5 (5 mg/kg) ( b , c ). ( a ) Western blotting analysis of tissues harvested from mice treated with Ac 4 ManAz, DCL-AAM or PBS. Tissue lysates were incubated with DBCO–Cy3 for 1 h at 37 °C before the gel was run. Protein bands were visualized using ImageQuant LAS 4010 system. ( b ) Ex vivo fluorescence imaging of tissues harvested from mice treated with DCL-AAM or PBS at 48 h p.i. of DBCO–Cy5. ( c ) Average Cy5 fluorescence intensity of tissues ( n = 3) from ( b ). ( d ) Normalized Cy5 fluorescence intensity of LS174T tumor tissues (20 sections per tumor, n = 3) harvested and sectioned from mice at 8, 24 and 48 h p.i. of DCL-AAM (60 mg/kg), respectively. Tumor tissue sections were labeled with DBCO–Cy5 for 30 min. ( e ) Normalized Cy5 fluorescence intensity of LS174T tumor tissues (20 sections per tumor, n = 3) harvested from mice with 0, 1, 2 and 3 DCL-AAM injections (60 mg/kg, 24 h interval). Tumor tissue sections were labeled with DBCO–Cy5 for 30 min. All the numerical data are presented as mean ± s.e.m. and analyzed by one-way ANOV A (Fisher; 0.01

    Journal: Nature chemical biology

    Article Title: Selective in vivo metabolic cell-labeling-mediated cancer targeting

    doi: 10.1038/nchembio.2297

    Figure Lengend Snippet: DCL-AAM mediated cancer-selective labeling in vivo ( a–c ) DCL-AAM (60 mg/kg), Ac 4 ManAz (40 mg/kg) or PB S was i.v. injected into athymic nude mice bearing subcutaneous LS174T tumors once daily for 3 d. At 24 h after the last injection, the metabolic expression of azido groups in tumor tissues was analyzed by western blotting ( a ) or by monitoring the biodistribution of i.v.-injected DBCO–Cy5 (5 mg/kg) ( b , c ). ( a ) Western blotting analysis of tissues harvested from mice treated with Ac 4 ManAz, DCL-AAM or PBS. Tissue lysates were incubated with DBCO–Cy3 for 1 h at 37 °C before the gel was run. Protein bands were visualized using ImageQuant LAS 4010 system. ( b ) Ex vivo fluorescence imaging of tissues harvested from mice treated with DCL-AAM or PBS at 48 h p.i. of DBCO–Cy5. ( c ) Average Cy5 fluorescence intensity of tissues ( n = 3) from ( b ). ( d ) Normalized Cy5 fluorescence intensity of LS174T tumor tissues (20 sections per tumor, n = 3) harvested and sectioned from mice at 8, 24 and 48 h p.i. of DCL-AAM (60 mg/kg), respectively. Tumor tissue sections were labeled with DBCO–Cy5 for 30 min. ( e ) Normalized Cy5 fluorescence intensity of LS174T tumor tissues (20 sections per tumor, n = 3) harvested from mice with 0, 1, 2 and 3 DCL-AAM injections (60 mg/kg, 24 h interval). Tumor tissue sections were labeled with DBCO–Cy5 for 30 min. All the numerical data are presented as mean ± s.e.m. and analyzed by one-way ANOV A (Fisher; 0.01

    Article Snippet: DBCO–Cy3 and DBCO–Cy5 were purchased from KeraFAST, Inc (Boston, MA, USA).

    Techniques: Labeling, In Vivo, Injection, Mouse Assay, Expressing, Western Blot, Incubation, Ex Vivo, Fluorescence, Imaging