datp-14-biotin Thermo Fisher Search Results


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  • 98
    Thermo Fisher biotin 14 datp
    Schematic illustration of the principle of antarctic thermal-sensitive uracil-DNA-glycosylase-supplemented polymerase spiral reaction (ATSU-PSR) technique for eliminating carryover contamination. Two steps are needed for ATSU-PSR technique for removing carryover contamination. During the first stage, all PSR complicons labeled with dUTP in the presence of Bst 2.0 enzyme and dUTP. During the second stage, all subsequent ATSU-PSR amplifications are digested using ATSU, which specifically cleave carryover contaminants by removing uracil in amplicons from previous reactions. Importantly, ATSU is heat inactivated during the PSR amplification stage (63°C), and the digested contaminants are degraded, ensuring that only the target templates are amplified. In addition, three components, including fluorescein isothiocyanate (FITC)-labeled primer, <t>biotin-14-dCTP,</t> and <t>biotin-14-dATP,</t> are added into ATSU-PSR mixtures for forming the biotin- and FITC-attached duplex products.
    Biotin 14 Datp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 2156 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher datp biotin
    Schematic illustration of the principle of antarctic thermal-sensitive uracil-DNA-glycosylase-supplemented polymerase spiral reaction (ATSU-PSR) technique for eliminating carryover contamination. Two steps are needed for ATSU-PSR technique for removing carryover contamination. During the first stage, all PSR complicons labeled with dUTP in the presence of Bst 2.0 enzyme and dUTP. During the second stage, all subsequent ATSU-PSR amplifications are digested using ATSU, which specifically cleave carryover contaminants by removing uracil in amplicons from previous reactions. Importantly, ATSU is heat inactivated during the PSR amplification stage (63°C), and the digested contaminants are degraded, ensuring that only the target templates are amplified. In addition, three components, including fluorescein isothiocyanate (FITC)-labeled primer, <t>biotin-14-dCTP,</t> and <t>biotin-14-dATP,</t> are added into ATSU-PSR mixtures for forming the biotin- and FITC-attached duplex products.
    Datp Biotin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Thermo Fisher biotin
    Experimental scheme and oligonucleotide sequences. ( A ) Principle behind the joining reaction. By exposing complementary sequences, vaccinia DNA polymerase promotes duplex joint formation. ( B ) Sequences of the oligonucleotide substrates used in this paper. Homologous sequences are shown in boldface. The nucleotides shown in lower case are incorporated post-synthetically using reverse transcriptase, cidofovir diphosphate or N 4 -modified <t>biotin-14-dCTP</t> (‘x’), and dATP or dTTP. Note that the primer–template design, in FC3, FC4 and FC5, prevents extension from the other 3′ end by this fill-in reaction. The 32 P label is indicated with an asterisk (‘*’).
    Biotin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 2927 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher biotin 14 ctp
    Experimental scheme and oligonucleotide sequences. ( A ) Principle behind the joining reaction. By exposing complementary sequences, vaccinia DNA polymerase promotes duplex joint formation. ( B ) Sequences of the oligonucleotide substrates used in this paper. Homologous sequences are shown in boldface. The nucleotides shown in lower case are incorporated post-synthetically using reverse transcriptase, cidofovir diphosphate or N 4 -modified <t>biotin-14-dCTP</t> (‘x’), and dATP or dTTP. Note that the primer–template design, in FC3, FC4 and FC5, prevents extension from the other 3′ end by this fill-in reaction. The 32 P label is indicated with an asterisk (‘*’).
    Biotin 14 Ctp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 392 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Thermo Fisher biotin 11 datp
    Experimental scheme and oligonucleotide sequences. ( A ) Principle behind the joining reaction. By exposing complementary sequences, vaccinia DNA polymerase promotes duplex joint formation. ( B ) Sequences of the oligonucleotide substrates used in this paper. Homologous sequences are shown in boldface. The nucleotides shown in lower case are incorporated post-synthetically using reverse transcriptase, cidofovir diphosphate or N 4 -modified <t>biotin-14-dCTP</t> (‘x’), and dATP or dTTP. Note that the primer–template design, in FC3, FC4 and FC5, prevents extension from the other 3′ end by this fill-in reaction. The 32 P label is indicated with an asterisk (‘*’).
    Biotin 11 Datp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher dctp ddctp biotin
    Experimental scheme and oligonucleotide sequences. ( A ) Principle behind the joining reaction. By exposing complementary sequences, vaccinia DNA polymerase promotes duplex joint formation. ( B ) Sequences of the oligonucleotide substrates used in this paper. Homologous sequences are shown in boldface. The nucleotides shown in lower case are incorporated post-synthetically using reverse transcriptase, cidofovir diphosphate or N 4 -modified <t>biotin-14-dCTP</t> (‘x’), and dATP or dTTP. Note that the primer–template design, in FC3, FC4 and FC5, prevents extension from the other 3′ end by this fill-in reaction. The 32 P label is indicated with an asterisk (‘*’).
    Dctp Ddctp Biotin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Schematic illustration of the principle of antarctic thermal-sensitive uracil-DNA-glycosylase-supplemented polymerase spiral reaction (ATSU-PSR) technique for eliminating carryover contamination. Two steps are needed for ATSU-PSR technique for removing carryover contamination. During the first stage, all PSR complicons labeled with dUTP in the presence of Bst 2.0 enzyme and dUTP. During the second stage, all subsequent ATSU-PSR amplifications are digested using ATSU, which specifically cleave carryover contaminants by removing uracil in amplicons from previous reactions. Importantly, ATSU is heat inactivated during the PSR amplification stage (63°C), and the digested contaminants are degraded, ensuring that only the target templates are amplified. In addition, three components, including fluorescein isothiocyanate (FITC)-labeled primer, biotin-14-dCTP, and biotin-14-dATP, are added into ATSU-PSR mixtures for forming the biotin- and FITC-attached duplex products.

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: Simultaneous Nucleic Acids Detection and Elimination of Carryover Contamination With Nanoparticles-Based Biosensor- and Antarctic Thermal Sensitive Uracil-DNA-Glycosylase-Supplemented Polymerase Spiral Reaction

    doi: 10.3389/fbioe.2019.00401

    Figure Lengend Snippet: Schematic illustration of the principle of antarctic thermal-sensitive uracil-DNA-glycosylase-supplemented polymerase spiral reaction (ATSU-PSR) technique for eliminating carryover contamination. Two steps are needed for ATSU-PSR technique for removing carryover contamination. During the first stage, all PSR complicons labeled with dUTP in the presence of Bst 2.0 enzyme and dUTP. During the second stage, all subsequent ATSU-PSR amplifications are digested using ATSU, which specifically cleave carryover contaminants by removing uracil in amplicons from previous reactions. Importantly, ATSU is heat inactivated during the PSR amplification stage (63°C), and the digested contaminants are degraded, ensuring that only the target templates are amplified. In addition, three components, including fluorescein isothiocyanate (FITC)-labeled primer, biotin-14-dCTP, and biotin-14-dATP, are added into ATSU-PSR mixtures for forming the biotin- and FITC-attached duplex products.

    Article Snippet: Biotin-14-dCTP and biotin-14-dATP were obtained from Thermo Scientific Co., Ltd. (Shanghai, China).

    Techniques: Labeling, Amplification

    Outline of nanoparticle-based biosensor-supplemented polymerase spiral reaction assay (NB-PSR). (A) Outline of PSR with Ft* primer, biotin-14-dCTP, and biotin-14-dATP. (B) The detailed structure of NB. (C) The schematic illustration of the principle of NB for visualization of PSR products. (D) Interpretation of the results: (I) positive (two red bands, including test line and control line, appeared on the visual region of NB); (II) negative (only the control line region showed a red band).

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: Simultaneous Nucleic Acids Detection and Elimination of Carryover Contamination With Nanoparticles-Based Biosensor- and Antarctic Thermal Sensitive Uracil-DNA-Glycosylase-Supplemented Polymerase Spiral Reaction

    doi: 10.3389/fbioe.2019.00401

    Figure Lengend Snippet: Outline of nanoparticle-based biosensor-supplemented polymerase spiral reaction assay (NB-PSR). (A) Outline of PSR with Ft* primer, biotin-14-dCTP, and biotin-14-dATP. (B) The detailed structure of NB. (C) The schematic illustration of the principle of NB for visualization of PSR products. (D) Interpretation of the results: (I) positive (two red bands, including test line and control line, appeared on the visual region of NB); (II) negative (only the control line region showed a red band).

    Article Snippet: Biotin-14-dCTP and biotin-14-dATP were obtained from Thermo Scientific Co., Ltd. (Shanghai, China).

    Techniques:

    Experimental scheme and oligonucleotide sequences. ( A ) Principle behind the joining reaction. By exposing complementary sequences, vaccinia DNA polymerase promotes duplex joint formation. ( B ) Sequences of the oligonucleotide substrates used in this paper. Homologous sequences are shown in boldface. The nucleotides shown in lower case are incorporated post-synthetically using reverse transcriptase, cidofovir diphosphate or N 4 -modified biotin-14-dCTP (‘x’), and dATP or dTTP. Note that the primer–template design, in FC3, FC4 and FC5, prevents extension from the other 3′ end by this fill-in reaction. The 32 P label is indicated with an asterisk (‘*’).

    Journal: Nucleic Acids Research

    Article Title: Duplex strand joining reactions catalyzed by vaccinia virus DNA polymerase

    doi: 10.1093/nar/gkl1015

    Figure Lengend Snippet: Experimental scheme and oligonucleotide sequences. ( A ) Principle behind the joining reaction. By exposing complementary sequences, vaccinia DNA polymerase promotes duplex joint formation. ( B ) Sequences of the oligonucleotide substrates used in this paper. Homologous sequences are shown in boldface. The nucleotides shown in lower case are incorporated post-synthetically using reverse transcriptase, cidofovir diphosphate or N 4 -modified biotin-14-dCTP (‘x’), and dATP or dTTP. Note that the primer–template design, in FC3, FC4 and FC5, prevents extension from the other 3′ end by this fill-in reaction. The 32 P label is indicated with an asterisk (‘*’).

    Article Snippet: Biotinylated oligonucleotides were prepared in the same way except we substituted 40 μM N4 -modified biotin-14-dCTP (Invitrogen) for cidofovir diphosphate.

    Techniques: Modification