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Image Search Results
Journal: Bioengineered
Article Title: Melatonin alleviates lipopolysaccharide (LPS) / adenosine triphosphate (ATP)-induced pyroptosis in rat alveolar Type II cells (RLE-6TN) through nuclear factor erythroid 2-related factor 2 (Nrf2)-driven reactive oxygen species (ROS) downregulation
doi: 10.1080/21655979.2021.2018981
Figure Lengend Snippet: Nrf2 activation is triggered through melatonin . (a and b) Corresponding immunoblots and the relative proteomic concentrations for nuclear Nrf2, cytoplasmic Nrf2, HO-1 and NQO1, and protein quantitative histogram shown on the right and below. (c) Immunofluorescence staining of nuclear Nrf2. (d) Representative immunoblots and relative protein levels of GSDMD-N and Caspase-1 P20, protein quantitative histogram shown on the right. (e) Determination of ROS production (scale bar: 500 µm). (f) Fluorescence microscopy images of DAPI (blue) and PI (red) (scale bar: 500 µm). (g) The OD ratio of PI/DAPI. (h) Determination of LDH release. (i) SiNrf2 downregulated proteomic expression of nuclear Nrf2. (j) SiNrf2 inhibited NLRP3 inflammasome activation. (k) Knockdown of Nrf2 increased the degree of PI-positive cells and (l) ROS production. Datasets represented as mean ± SD (n = 3) per group. * P < 0.05, ** P < 0.01, ns, P > 0.05, compared to the control group. # P < 0.05, NS, P > 0.05, compared to the LPS/ATP group. $ P < 0.05, in comparison to LPS+ATP+Mel group. & P < 0.05, compared to LPS+ATP+Mel+NC group.
Article Snippet: Eventually,
Techniques: Activation Assay, Western Blot, Immunofluorescence, Staining, Fluorescence, Microscopy, Expressing, Knockdown, Control, Comparison