damgo Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Millipore damgo
    Effect of <t>β-FNA</t> on <t>DAMGO-stimulated</t> [ 35 H]GTP-γ-S binding in the lateral amygdala
    Damgo, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 385 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/damgo/product/Millipore
    Average 99 stars, based on 385 article reviews
    Price from $9.99 to $1999.99
    damgo - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    94
    Tocris damgo
    Selective and non-specific binding for MOR and KOR autoradiography. 1A) Selective binding of MOR using [ 3 <t>H]DAMGO</t> blocking DOR and KOR by co-incubating with <t>DPDPE</t> and U69,593, respectively 1B) Non-specific binding of MOR with [ 3 H]DAMGO and co-incubating
    Damgo, supplied by Tocris, used in various techniques. Bioz Stars score: 94/100, based on 218 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/damgo/product/Tocris
    Average 94 stars, based on 218 article reviews
    Price from $9.99 to $1999.99
    damgo - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    damgo  (Abcam)
    91
    Abcam damgo
    Sex differences in behavioral consequences of activating μ -opioid receptor (MOR) in the locus coeruleus (LC). (a and b) Effects of ACSF and <t>DAMGO</t> ( D <t>-Ala2,</t> N -MePhe4, Gly-ol]-enkephalin; 3 and 10 pg) bilaterally infused into the LC of male (a) and female (b) rats on performance in the operant strategy set-shifting task. The bars represent the mean number of trials necessary to reach the criterion for side discrimination, side reversal and shift to light stages of the task. Vertical lines represent SEM. The number of subjects is indicated in the graph legend. Asterisks above the bars indicate that both DAMGO doses were associated with increased trials to reach criterion compared to ACSF ( p
    Damgo, supplied by Abcam, used in various techniques. Bioz Stars score: 91/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/damgo/product/Abcam
    Average 91 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    damgo - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    92
    PerkinElmer damgo
    Sex differences in behavioral consequences of activating μ -opioid receptor (MOR) in the locus coeruleus (LC). (a and b) Effects of ACSF and <t>DAMGO</t> ( D <t>-Ala2,</t> N -MePhe4, Gly-ol]-enkephalin; 3 and 10 pg) bilaterally infused into the LC of male (a) and female (b) rats on performance in the operant strategy set-shifting task. The bars represent the mean number of trials necessary to reach the criterion for side discrimination, side reversal and shift to light stages of the task. Vertical lines represent SEM. The number of subjects is indicated in the graph legend. Asterisks above the bars indicate that both DAMGO doses were associated with increased trials to reach criterion compared to ACSF ( p
    Damgo, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 92/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/damgo/product/PerkinElmer
    Average 92 stars, based on 69 article reviews
    Price from $9.99 to $1999.99
    damgo - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    92
    GE Healthcare damgo
    Sex differences in behavioral consequences of activating μ -opioid receptor (MOR) in the locus coeruleus (LC). (a and b) Effects of ACSF and <t>DAMGO</t> ( D <t>-Ala2,</t> N -MePhe4, Gly-ol]-enkephalin; 3 and 10 pg) bilaterally infused into the LC of male (a) and female (b) rats on performance in the operant strategy set-shifting task. The bars represent the mean number of trials necessary to reach the criterion for side discrimination, side reversal and shift to light stages of the task. Vertical lines represent SEM. The number of subjects is indicated in the graph legend. Asterisks above the bars indicate that both DAMGO doses were associated with increased trials to reach criterion compared to ACSF ( p
    Damgo, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/damgo/product/GE Healthcare
    Average 92 stars, based on 31 article reviews
    Price from $9.99 to $1999.99
    damgo - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    92
    Peninsula Laboratories damgo
    Sex differences in behavioral consequences of activating μ -opioid receptor (MOR) in the locus coeruleus (LC). (a and b) Effects of ACSF and <t>DAMGO</t> ( D <t>-Ala2,</t> N -MePhe4, Gly-ol]-enkephalin; 3 and 10 pg) bilaterally infused into the LC of male (a) and female (b) rats on performance in the operant strategy set-shifting task. The bars represent the mean number of trials necessary to reach the criterion for side discrimination, side reversal and shift to light stages of the task. Vertical lines represent SEM. The number of subjects is indicated in the graph legend. Asterisks above the bars indicate that both DAMGO doses were associated with increased trials to reach criterion compared to ACSF ( p
    Damgo, supplied by Peninsula Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/damgo/product/Peninsula Laboratories
    Average 92 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    damgo - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    92
    R&D Systems damgo
    Effect of <t>DAMGO,</t> loperamide and eluxadoline on G-protein activation. (A–C) Membranes (10 μg) from spinal cords of WT, μOR −/− and δOR −/− mice were subjected to a [ 35 <t>S]GTPγS</t> binding assay using DAMGO (A), loperamide (B), and eluxadoline (C) (0–10 μM final concentration) as described in Section 2. (D–G) Membranes (20 μg) from the ileum of WT mice were subjected to a [ 35 S]GTPγS binding assay using DAMGO (D and G), loperamide (E and G), and eluxadoline (F and G) (0–10 μM final concentration) in the presence or absence of TIPPψ (10 nM final concentration) as described in Section 2. (G) Represents E max (% of basal) obtained with 10 μM final concentration of DAMGO (±10 nM final concentration of TIPPψ), eluxadoline or loperamide. Basal values determined in the absence of the agonist were taken as 100%. Results are the mean ± S.E.M. n = 3–9. n.d., Not determined. * p
    Damgo, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/damgo/product/R&D Systems
    Average 92 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    damgo - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    92
    Phoenix Pharmaceuticals damgo
    <t>Endothelin</t> effects on channel mutants A , diagrams depict channel mutants: Kir3.4 (S143T) parent, N-terminal truncation, and chimeras. We produced the Kir3.4(S143T) using a cDNA template for cRNA coding a Kir3.4(S143T) having a truncated (1–57) N terminus. We used Kir3 chimeras: Kir3.4(S143T)-(1–338)/Kir3.1-(333–501) and Kir3.4(S143T)-(1–249)/Kir3.1-(244–501). B , oocytes were injected with 1 ng of MOR and 1 ng of HETAR, and either 1 ng of Kir3.4(S143T), 1 ng of Kir3.4(S143T)-(Δ1–57), 1 ng of Kir3.4(S143T)-(1–338)/Kir3.1-(333–501), or 1 ng of Kir3.4(S143T)-(1–249)/Kir3.1-(244–501). The percent inhibition of the second mu opioid response after Et-1 pretreatment is shown. The bar graph summarizes the effects of Et-1 on the <t>DAMGO</t> activation of MOR compared with untreated controls from the same batch. Data are means ± S.E. from four to seven oocytes and two to three independent experiments.
    Damgo, supplied by Phoenix Pharmaceuticals, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/damgo/product/Phoenix Pharmaceuticals
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    damgo - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    89
    American Radiolabeled Chemicals Inc h damgo
    <t>Endothelin</t> effects on channel mutants A , diagrams depict channel mutants: Kir3.4 (S143T) parent, N-terminal truncation, and chimeras. We produced the Kir3.4(S143T) using a cDNA template for cRNA coding a Kir3.4(S143T) having a truncated (1–57) N terminus. We used Kir3 chimeras: Kir3.4(S143T)-(1–338)/Kir3.1-(333–501) and Kir3.4(S143T)-(1–249)/Kir3.1-(244–501). B , oocytes were injected with 1 ng of MOR and 1 ng of HETAR, and either 1 ng of Kir3.4(S143T), 1 ng of Kir3.4(S143T)-(Δ1–57), 1 ng of Kir3.4(S143T)-(1–338)/Kir3.1-(333–501), or 1 ng of Kir3.4(S143T)-(1–249)/Kir3.1-(244–501). The percent inhibition of the second mu opioid response after Et-1 pretreatment is shown. The bar graph summarizes the effects of Et-1 on the <t>DAMGO</t> activation of MOR compared with untreated controls from the same batch. Data are means ± S.E. from four to seven oocytes and two to three independent experiments.
    H Damgo, supplied by American Radiolabeled Chemicals Inc, used in various techniques. Bioz Stars score: 89/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/h damgo/product/American Radiolabeled Chemicals Inc
    Average 89 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    h damgo - by Bioz Stars, 2020-08
    89/100 stars
      Buy from Supplier

    88
    Quantum Dot Inc nanoruby damgo
    <t>Endothelin</t> effects on channel mutants A , diagrams depict channel mutants: Kir3.4 (S143T) parent, N-terminal truncation, and chimeras. We produced the Kir3.4(S143T) using a cDNA template for cRNA coding a Kir3.4(S143T) having a truncated (1–57) N terminus. We used Kir3 chimeras: Kir3.4(S143T)-(1–338)/Kir3.1-(333–501) and Kir3.4(S143T)-(1–249)/Kir3.1-(244–501). B , oocytes were injected with 1 ng of MOR and 1 ng of HETAR, and either 1 ng of Kir3.4(S143T), 1 ng of Kir3.4(S143T)-(Δ1–57), 1 ng of Kir3.4(S143T)-(1–338)/Kir3.1-(333–501), or 1 ng of Kir3.4(S143T)-(1–249)/Kir3.1-(244–501). The percent inhibition of the second mu opioid response after Et-1 pretreatment is shown. The bar graph summarizes the effects of Et-1 on the <t>DAMGO</t> activation of MOR compared with untreated controls from the same batch. Data are means ± S.E. from four to seven oocytes and two to three independent experiments.
    Nanoruby Damgo, supplied by Quantum Dot Inc, used in various techniques. Bioz Stars score: 88/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nanoruby damgo/product/Quantum Dot Inc
    Average 88 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    nanoruby damgo - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    89
    PerkinElmer h damgo
    Opioid receptor antagonist and agonist modulation MOR- or DOR-specific ligand binding in NK cells. Shown are representative saturation curves ( A, C, E, and G ) and the B max ( B, D, F, and H ) of MOR-specific ligand [ 3 <t>H]DAMGO</t> or DOR-specific ligand [ 3 <t>H]naltrindole.</t>
    H Damgo, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 89/100, based on 76 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/h damgo/product/PerkinElmer
    Average 89 stars, based on 76 article reviews
    Price from $9.99 to $1999.99
    h damgo - by Bioz Stars, 2020-08
    89/100 stars
      Buy from Supplier

    89
    GE Healthcare h damgo
    Opioid receptor antagonist and agonist modulation MOR- or DOR-specific ligand binding in NK cells. Shown are representative saturation curves ( A, C, E, and G ) and the B max ( B, D, F, and H ) of MOR-specific ligand [ 3 <t>H]DAMGO</t> or DOR-specific ligand [ 3 <t>H]naltrindole.</t>
    H Damgo, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 89/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/h damgo/product/GE Healthcare
    Average 89 stars, based on 34 article reviews
    Price from $9.99 to $1999.99
    h damgo - by Bioz Stars, 2020-08
    89/100 stars
      Buy from Supplier

    92
    Bachem damgo
    Relative efficacy values for <t>DAMGO,</t> <t>etorphine,</t> endomorphin-2, and morphine for five MOPr signaling outputs. Values refer to efficacy relative to that of DAMGO, which was set to 1.00 for each output. The values for [ 35 S]GTPγS binding and arrestin-3
    Damgo, supplied by Bachem, used in various techniques. Bioz Stars score: 92/100, based on 70 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/damgo/product/Bachem
    Average 92 stars, based on 70 article reviews
    Price from $9.99 to $1999.99
    damgo - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    93
    Hello Bio Inc damgo
    Relative efficacy values for <t>DAMGO,</t> <t>etorphine,</t> endomorphin-2, and morphine for five MOPr signaling outputs. Values refer to efficacy relative to that of DAMGO, which was set to 1.00 for each output. The values for [ 35 S]GTPγS binding and arrestin-3
    Damgo, supplied by Hello Bio Inc, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/damgo/product/Hello Bio Inc
    Average 93 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    damgo - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    92
    Sanofi damgo
    Activation of PKC promotes a selective <t>MOR</t> phosphorylation at T370. (A) HEK239 cells stably expressing MOR were either not exposed (−) or exposed to 1 nM, 10 nM, 100 nM, 1 μM or 10 μM PMA for 30 min. Cells were lysed and immunoblotted with anti-pS363, anti-pT370, anti-pS375, anti-pT376 and anti-pT379 antibodies. Blots were stripped and reprobed with the phosphorylation-independent anti-MOR antibody {UMB-3} to confirm equal loading of the gel. Note that PMA stimulates a dose-dependent phosphorylation of T370. S363 is constitutively phosphorylated. (B) Cells were stably exposed to 10 μM <t>DAMGO</t> or 1 μM PMA for 0, 2, 5, 10, 20 or 30 min, and then immunoblotted with anti-pS375 or anti-pT370 antibodies. Blots were stripped and reprobed with the phosphorylation-independent anti-MOR antibody {UMB-3}. Representative results from one of four independent experiments per condition are shown. The position of molecular mass markers is indicated on the left (in kDa). (C) MOR-and T370A-expressing HEK293 cells were either not exposed (−) or exposed to 10 μM DAMGO or 1 μM PMA for 30 min. Cells were then stained with anti-HA antibody, processed for immunofluorescence and examined by confocal microscopy. Note that PMA induces internalization of wild-type MOR but not of the T370A mutant. Representative images from one of three independent experiments performed in duplicate are shown. Scale bar: 20 μm.
    Damgo, supplied by Sanofi, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/damgo/product/Sanofi
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    damgo - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    96
    Millipore reagents damgo
    Activation of PKC promotes a selective <t>MOR</t> phosphorylation at T370. (A) HEK239 cells stably expressing MOR were either not exposed (−) or exposed to 1 nM, 10 nM, 100 nM, 1 μM or 10 μM PMA for 30 min. Cells were lysed and immunoblotted with anti-pS363, anti-pT370, anti-pS375, anti-pT376 and anti-pT379 antibodies. Blots were stripped and reprobed with the phosphorylation-independent anti-MOR antibody {UMB-3} to confirm equal loading of the gel. Note that PMA stimulates a dose-dependent phosphorylation of T370. S363 is constitutively phosphorylated. (B) Cells were stably exposed to 10 μM <t>DAMGO</t> or 1 μM PMA for 0, 2, 5, 10, 20 or 30 min, and then immunoblotted with anti-pS375 or anti-pT370 antibodies. Blots were stripped and reprobed with the phosphorylation-independent anti-MOR antibody {UMB-3}. Representative results from one of four independent experiments per condition are shown. The position of molecular mass markers is indicated on the left (in kDa). (C) MOR-and T370A-expressing HEK293 cells were either not exposed (−) or exposed to 10 μM DAMGO or 1 μM PMA for 30 min. Cells were then stained with anti-HA antibody, processed for immunofluorescence and examined by confocal microscopy. Note that PMA induces internalization of wild-type MOR but not of the T370A mutant. Representative images from one of three independent experiments performed in duplicate are shown. Scale bar: 20 μm.
    Reagents Damgo, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reagents damgo/product/Millipore
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    reagents damgo - by Bioz Stars, 2020-08
    96/100 stars
      Buy from Supplier

    93
    Millipore antibodies damgo
    Activation of PKC promotes a selective <t>MOR</t> phosphorylation at T370. (A) HEK239 cells stably expressing MOR were either not exposed (−) or exposed to 1 nM, 10 nM, 100 nM, 1 μM or 10 μM PMA for 30 min. Cells were lysed and immunoblotted with anti-pS363, anti-pT370, anti-pS375, anti-pT376 and anti-pT379 antibodies. Blots were stripped and reprobed with the phosphorylation-independent anti-MOR antibody {UMB-3} to confirm equal loading of the gel. Note that PMA stimulates a dose-dependent phosphorylation of T370. S363 is constitutively phosphorylated. (B) Cells were stably exposed to 10 μM <t>DAMGO</t> or 1 μM PMA for 0, 2, 5, 10, 20 or 30 min, and then immunoblotted with anti-pS375 or anti-pT370 antibodies. Blots were stripped and reprobed with the phosphorylation-independent anti-MOR antibody {UMB-3}. Representative results from one of four independent experiments per condition are shown. The position of molecular mass markers is indicated on the left (in kDa). (C) MOR-and T370A-expressing HEK293 cells were either not exposed (−) or exposed to 10 μM DAMGO or 1 μM PMA for 30 min. Cells were then stained with anti-HA antibody, processed for immunofluorescence and examined by confocal microscopy. Note that PMA induces internalization of wild-type MOR but not of the T370A mutant. Representative images from one of three independent experiments performed in duplicate are shown. Scale bar: 20 μm.
    Antibodies Damgo, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies damgo/product/Millipore
    Average 93 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    antibodies damgo - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    85
    Millipore damgo acetate salt
    Activation of PKC promotes a selective <t>MOR</t> phosphorylation at T370. (A) HEK239 cells stably expressing MOR were either not exposed (−) or exposed to 1 nM, 10 nM, 100 nM, 1 μM or 10 μM PMA for 30 min. Cells were lysed and immunoblotted with anti-pS363, anti-pT370, anti-pS375, anti-pT376 and anti-pT379 antibodies. Blots were stripped and reprobed with the phosphorylation-independent anti-MOR antibody {UMB-3} to confirm equal loading of the gel. Note that PMA stimulates a dose-dependent phosphorylation of T370. S363 is constitutively phosphorylated. (B) Cells were stably exposed to 10 μM <t>DAMGO</t> or 1 μM PMA for 0, 2, 5, 10, 20 or 30 min, and then immunoblotted with anti-pS375 or anti-pT370 antibodies. Blots were stripped and reprobed with the phosphorylation-independent anti-MOR antibody {UMB-3}. Representative results from one of four independent experiments per condition are shown. The position of molecular mass markers is indicated on the left (in kDa). (C) MOR-and T370A-expressing HEK293 cells were either not exposed (−) or exposed to 10 μM DAMGO or 1 μM PMA for 30 min. Cells were then stained with anti-HA antibody, processed for immunofluorescence and examined by confocal microscopy. Note that PMA induces internalization of wild-type MOR but not of the T370A mutant. Representative images from one of three independent experiments performed in duplicate are shown. Scale bar: 20 μm.
    Damgo Acetate Salt, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/damgo acetate salt/product/Millipore
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    damgo acetate salt - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    94
    Millipore drugs damgo
    Activation of PKC promotes a selective <t>MOR</t> phosphorylation at T370. (A) HEK239 cells stably expressing MOR were either not exposed (−) or exposed to 1 nM, 10 nM, 100 nM, 1 μM or 10 μM PMA for 30 min. Cells were lysed and immunoblotted with anti-pS363, anti-pT370, anti-pS375, anti-pT376 and anti-pT379 antibodies. Blots were stripped and reprobed with the phosphorylation-independent anti-MOR antibody {UMB-3} to confirm equal loading of the gel. Note that PMA stimulates a dose-dependent phosphorylation of T370. S363 is constitutively phosphorylated. (B) Cells were stably exposed to 10 μM <t>DAMGO</t> or 1 μM PMA for 0, 2, 5, 10, 20 or 30 min, and then immunoblotted with anti-pS375 or anti-pT370 antibodies. Blots were stripped and reprobed with the phosphorylation-independent anti-MOR antibody {UMB-3}. Representative results from one of four independent experiments per condition are shown. The position of molecular mass markers is indicated on the left (in kDa). (C) MOR-and T370A-expressing HEK293 cells were either not exposed (−) or exposed to 10 μM DAMGO or 1 μM PMA for 30 min. Cells were then stained with anti-HA antibody, processed for immunofluorescence and examined by confocal microscopy. Note that PMA induces internalization of wild-type MOR but not of the T370A mutant. Representative images from one of three independent experiments performed in duplicate are shown. Scale bar: 20 μm.
    Drugs Damgo, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/drugs damgo/product/Millipore
    Average 94 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    drugs damgo - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    85
    PerkinElmer 3h damgo
    Activation of PKC promotes a selective <t>MOR</t> phosphorylation at T370. (A) HEK239 cells stably expressing MOR were either not exposed (−) or exposed to 1 nM, 10 nM, 100 nM, 1 μM or 10 μM PMA for 30 min. Cells were lysed and immunoblotted with anti-pS363, anti-pT370, anti-pS375, anti-pT376 and anti-pT379 antibodies. Blots were stripped and reprobed with the phosphorylation-independent anti-MOR antibody {UMB-3} to confirm equal loading of the gel. Note that PMA stimulates a dose-dependent phosphorylation of T370. S363 is constitutively phosphorylated. (B) Cells were stably exposed to 10 μM <t>DAMGO</t> or 1 μM PMA for 0, 2, 5, 10, 20 or 30 min, and then immunoblotted with anti-pS375 or anti-pT370 antibodies. Blots were stripped and reprobed with the phosphorylation-independent anti-MOR antibody {UMB-3}. Representative results from one of four independent experiments per condition are shown. The position of molecular mass markers is indicated on the left (in kDa). (C) MOR-and T370A-expressing HEK293 cells were either not exposed (−) or exposed to 10 μM DAMGO or 1 μM PMA for 30 min. Cells were then stained with anti-HA antibody, processed for immunofluorescence and examined by confocal microscopy. Note that PMA induces internalization of wild-type MOR but not of the T370A mutant. Representative images from one of three independent experiments performed in duplicate are shown. Scale bar: 20 μm.
    3h Damgo, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 85/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/3h damgo/product/PerkinElmer
    Average 85 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    3h damgo - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    Image Search Results


    Effect of β-FNA on DAMGO-stimulated [ 35 H]GTP-γ-S binding in the lateral amygdala

    Journal: Anesthesiology

    Article Title: Involvement of the Lateral Amygdala in the Antiallodynic and Reinforcing Effects of Heroin in Rats after Peripheral Nerve Injury

    doi: 10.1097/ALN.0b013e318209aba7

    Figure Lengend Snippet: Effect of β-FNA on DAMGO-stimulated [ 35 H]GTP-γ-S binding in the lateral amygdala

    Article Snippet: Isoflurane was purchased from Halocarbon Products (North Augusta, SC). β-funaltrexamine and DAMGO were purchased from Sigma-Aldrich (St. Louis, MO) and solutions were prepared fresh as needed in sterile 0.9% w/v sodium chloride or assay buffer.

    Techniques: Binding Assay

    Selective and non-specific binding for MOR and KOR autoradiography. 1A) Selective binding of MOR using [ 3 H]DAMGO blocking DOR and KOR by co-incubating with DPDPE and U69,593, respectively 1B) Non-specific binding of MOR with [ 3 H]DAMGO and co-incubating

    Journal: Neuroscience

    Article Title: μ and κ Opioid receptor distribution in the monogamous titi monkey (Callicebus cupreus): Implications for social behavior and endocrine functioning

    doi: 10.1016/j.neuroscience.2015.01.023

    Figure Lengend Snippet: Selective and non-specific binding for MOR and KOR autoradiography. 1A) Selective binding of MOR using [ 3 H]DAMGO blocking DOR and KOR by co-incubating with DPDPE and U69,593, respectively 1B) Non-specific binding of MOR with [ 3 H]DAMGO and co-incubating

    Article Snippet: Sections were dipped in Tris buffer (50mM Tris base; pH 7.4) for 60 minutes at room temperature then incubated for 90 minutes in 3.0 nM [3 H]U69,593 (PerkinElmer, Inc., Boston, MA) and co-incubated with 400nM of DPDPE and 400nM DAMGO (Tocris Bioscience, Minneapolis, MN) at room temperature to block DOR and MOR, respectively.

    Techniques: Binding Assay, Autoradiography, Blocking Assay

    Spinal μ-opioid receptor (MOR) activation reduces nociceptive responses in all ages. (A) Typical electromyographic (EMG) traces in postnatal day (P)10, P21, and adult rats during baseline (without any stimulation), predrug (a typical threshold response before application of drug) and postdrug (A: DAMGO 30 ng; B: CTOP 120 ng) periods. The application of MOR opioid agonist DAMGO onto the spinal cord produced antinociceptive responses in all ages tested. Spinal reflex excitability (C) was decreased when compared to age-matched saline and predrug responses in all ages, both saline and CTOP had no significant effect. Mechanical threshold (D) was increased when DAMGO was administered in P10 and P21 rats. ++ , ++++ P

    Journal: Pain

    Article Title: Postnatal maturation of endogenous opioid systems within the periaqueductal grey and spinal dorsal horn of the rat

    doi: 10.1016/j.pain.2013.09.022

    Figure Lengend Snippet: Spinal μ-opioid receptor (MOR) activation reduces nociceptive responses in all ages. (A) Typical electromyographic (EMG) traces in postnatal day (P)10, P21, and adult rats during baseline (without any stimulation), predrug (a typical threshold response before application of drug) and postdrug (A: DAMGO 30 ng; B: CTOP 120 ng) periods. The application of MOR opioid agonist DAMGO onto the spinal cord produced antinociceptive responses in all ages tested. Spinal reflex excitability (C) was decreased when compared to age-matched saline and predrug responses in all ages, both saline and CTOP had no significant effect. Mechanical threshold (D) was increased when DAMGO was administered in P10 and P21 rats. ++ , ++++ P

    Article Snippet: Drugs DAMGO (MOR-agonist, 30 ng; Tocris, Abingdon, Oxon, UK) and CTOP (MOR-antagonist, 100 ng; Tocris) were administered at doses determined from previously published studies in adult brainstem .

    Techniques: Activation Assay, Produced

    Recruitment of β-arrestin2 by activation of μ-opioid receptors. Stably transfected HEK293 cells coexpressing μ-opioid receptors and β-arrestin2 were pretreated for 60 min with DAMGO or PnPP-19 at the indicated concentrations. The group “DAMGO + PnPP-19” represents prior incubation of the cells with 10 μM of PnPP-19 for 30 min. β-arrestin2 recruitment was quantified by high content imaging complementation assay as described in Materials and Methods ( n = 5).

    Journal: Toxins

    Article Title: The Peptide PnPP-19, a Spider Toxin Derivative, Activates μ-Opioid Receptors and Modulates Calcium Channels

    doi: 10.3390/toxins10010043

    Figure Lengend Snippet: Recruitment of β-arrestin2 by activation of μ-opioid receptors. Stably transfected HEK293 cells coexpressing μ-opioid receptors and β-arrestin2 were pretreated for 60 min with DAMGO or PnPP-19 at the indicated concentrations. The group “DAMGO + PnPP-19” represents prior incubation of the cells with 10 μM of PnPP-19 for 30 min. β-arrestin2 recruitment was quantified by high content imaging complementation assay as described in Materials and Methods ( n = 5).

    Article Snippet: Plates were kept in a humidified incubator at 37 °C filled with 5% CO2 for 24 h. HEK293T were stimulated with the selective opioid agonist DAMGO (Tocris, Minneapolis, MN, USA) or the synthetic peptide PnPP-19 in HEPES-buffered saline solution (HBSS) including 0.1% v / v BSA (10−10 M–10−4 M) for 60 min at 37 °C.

    Techniques: Activation Assay, Stable Transfection, Transfection, Incubation, Imaging

    Modulation of spinal reflex amplitudes (SRAs) by the MOR-preferring agonist, DAMGO. (A) In WT, DAMGO induced a significant decrease in SRA (to 66.6 ± 4.5% of control, n = 15). WT: * p

    Journal: Frontiers in Neural Circuits

    Article Title: Dopamine D3 receptor dysfunction prevents anti-nociceptive effects of morphine in the spinal cord

    doi: 10.3389/fncir.2014.00062

    Figure Lengend Snippet: Modulation of spinal reflex amplitudes (SRAs) by the MOR-preferring agonist, DAMGO. (A) In WT, DAMGO induced a significant decrease in SRA (to 66.6 ± 4.5% of control, n = 15). WT: * p

    Article Snippet: SRAs were obtained before (control conditions) and during application of morphine sulfate (Sigma, 1 μM), the D3 receptor-preferring antagonist, nafadotride (Tocris, 20 μM), or the specific MOR agonist DAMGO (Tocris, 10 μM).

    Techniques:

    Sex differences in behavioral consequences of activating μ -opioid receptor (MOR) in the locus coeruleus (LC). (a and b) Effects of ACSF and DAMGO ( D -Ala2, N -MePhe4, Gly-ol]-enkephalin; 3 and 10 pg) bilaterally infused into the LC of male (a) and female (b) rats on performance in the operant strategy set-shifting task. The bars represent the mean number of trials necessary to reach the criterion for side discrimination, side reversal and shift to light stages of the task. Vertical lines represent SEM. The number of subjects is indicated in the graph legend. Asterisks above the bars indicate that both DAMGO doses were associated with increased trials to reach criterion compared to ACSF ( p

    Journal: Neuropsychopharmacology

    Article Title: Sex Differences in μ-Opioid Receptor Regulation of the Rat Locus Coeruleus and Their Cognitive Consequences

    doi: 10.1038/npp.2016.252

    Figure Lengend Snippet: Sex differences in behavioral consequences of activating μ -opioid receptor (MOR) in the locus coeruleus (LC). (a and b) Effects of ACSF and DAMGO ( D -Ala2, N -MePhe4, Gly-ol]-enkephalin; 3 and 10 pg) bilaterally infused into the LC of male (a) and female (b) rats on performance in the operant strategy set-shifting task. The bars represent the mean number of trials necessary to reach the criterion for side discrimination, side reversal and shift to light stages of the task. Vertical lines represent SEM. The number of subjects is indicated in the graph legend. Asterisks above the bars indicate that both DAMGO doses were associated with increased trials to reach criterion compared to ACSF ( p

    Article Snippet: Double-barrel glass micropipettes were used for simultaneous recording and microinfusion of DAMGO ([D -Ala2, N -MePhe4, Gly-ol]-enkephalin; Abcam, Cambridge, MA), a synthetic opioid peptide with high MOR specificity.

    Techniques:

    Regional specificity of DAMGO ( D -Ala2, N -MePhe4, Gly-ol]-enkephalin) effects on strategy shifting. (a) Photomicrograph of a Neutral Red counterstained section through the LC showing histological verification of the injection site from a representative animal that was injected with DAMGO. The arrowhead points to the LC and the arrow points to the dye, which is localized to the LC. Cb, cerebellum; V, ventricle. (b) Plots of accurate (circles) and missed (squares) injection sites for DAMGO (3 pg) for males (black) and females (red). DAMGO effects from these cases were used for the graphs in c and d. (c) Comparison of the effects of DAMGO (3 pg) microinfused into the LC of male rats (in, n =8), outside of the LC (out, n =5) and ACSF ( n =9) on performance in different components of the OSST. The bars indicate the number of trials necessary to reach the criterion for each stage. Vertical lines represent SEM. Asterisks indicate a significant difference compared with both the ACSF and DAMGO out groups ( p

    Journal: Neuropsychopharmacology

    Article Title: Sex Differences in μ-Opioid Receptor Regulation of the Rat Locus Coeruleus and Their Cognitive Consequences

    doi: 10.1038/npp.2016.252

    Figure Lengend Snippet: Regional specificity of DAMGO ( D -Ala2, N -MePhe4, Gly-ol]-enkephalin) effects on strategy shifting. (a) Photomicrograph of a Neutral Red counterstained section through the LC showing histological verification of the injection site from a representative animal that was injected with DAMGO. The arrowhead points to the LC and the arrow points to the dye, which is localized to the LC. Cb, cerebellum; V, ventricle. (b) Plots of accurate (circles) and missed (squares) injection sites for DAMGO (3 pg) for males (black) and females (red). DAMGO effects from these cases were used for the graphs in c and d. (c) Comparison of the effects of DAMGO (3 pg) microinfused into the LC of male rats (in, n =8), outside of the LC (out, n =5) and ACSF ( n =9) on performance in different components of the OSST. The bars indicate the number of trials necessary to reach the criterion for each stage. Vertical lines represent SEM. Asterisks indicate a significant difference compared with both the ACSF and DAMGO out groups ( p

    Article Snippet: Double-barrel glass micropipettes were used for simultaneous recording and microinfusion of DAMGO ([D -Ala2, N -MePhe4, Gly-ol]-enkephalin; Abcam, Cambridge, MA), a synthetic opioid peptide with high MOR specificity.

    Techniques: Injection

    Dose-related inhibition of locus coeruleus (LC) neuronal discharge rate by DAMGO ( D -Ala2, N -MePhe4, Gly-ol]-enkephalin) in male and female rats. (a and b) Line graphs show the time course of DAMGO effects on LC discharge rate. The abscissae indicate time (s) before and after DAMGO, which was administered at time=0. The ordinates indicate LC discharge rate expressed as a percentage of the baseline rate before DAMGO. For 0.1 pg: males ( n =6 cells/3 rats), females ( n =3 cells/3 rats); for 1 pg: males ( n =7 cells/6 rats), females ( n =6 cells/4 rats); for 10 pg: males ( n =7 cells/5 rats), females ( n =7 cells/5 rats); for 30 pg females ( n =10 cells/6 rats). (c and d) Representative ratemeter records from a single locus coeruleus neuron of a (c) male and (d) female rat before and after DAMGO 10 pg microinfusion into the LC (indicated by the bars above the traces).

    Journal: Neuropsychopharmacology

    Article Title: Sex Differences in μ-Opioid Receptor Regulation of the Rat Locus Coeruleus and Their Cognitive Consequences

    doi: 10.1038/npp.2016.252

    Figure Lengend Snippet: Dose-related inhibition of locus coeruleus (LC) neuronal discharge rate by DAMGO ( D -Ala2, N -MePhe4, Gly-ol]-enkephalin) in male and female rats. (a and b) Line graphs show the time course of DAMGO effects on LC discharge rate. The abscissae indicate time (s) before and after DAMGO, which was administered at time=0. The ordinates indicate LC discharge rate expressed as a percentage of the baseline rate before DAMGO. For 0.1 pg: males ( n =6 cells/3 rats), females ( n =3 cells/3 rats); for 1 pg: males ( n =7 cells/6 rats), females ( n =6 cells/4 rats); for 10 pg: males ( n =7 cells/5 rats), females ( n =7 cells/5 rats); for 30 pg females ( n =10 cells/6 rats). (c and d) Representative ratemeter records from a single locus coeruleus neuron of a (c) male and (d) female rat before and after DAMGO 10 pg microinfusion into the LC (indicated by the bars above the traces).

    Article Snippet: Double-barrel glass micropipettes were used for simultaneous recording and microinfusion of DAMGO ([D -Ala2, N -MePhe4, Gly-ol]-enkephalin; Abcam, Cambridge, MA), a synthetic opioid peptide with high MOR specificity.

    Techniques: Inhibition

    Effect of DAMGO, loperamide and eluxadoline on G-protein activation. (A–C) Membranes (10 μg) from spinal cords of WT, μOR −/− and δOR −/− mice were subjected to a [ 35 S]GTPγS binding assay using DAMGO (A), loperamide (B), and eluxadoline (C) (0–10 μM final concentration) as described in Section 2. (D–G) Membranes (20 μg) from the ileum of WT mice were subjected to a [ 35 S]GTPγS binding assay using DAMGO (D and G), loperamide (E and G), and eluxadoline (F and G) (0–10 μM final concentration) in the presence or absence of TIPPψ (10 nM final concentration) as described in Section 2. (G) Represents E max (% of basal) obtained with 10 μM final concentration of DAMGO (±10 nM final concentration of TIPPψ), eluxadoline or loperamide. Basal values determined in the absence of the agonist were taken as 100%. Results are the mean ± S.E.M. n = 3–9. n.d., Not determined. * p

    Journal: Biochemical pharmacology

    Article Title: Molecular characterization of eluxadoline as a potential ligand targeting mu-delta opioid receptor heteromers

    doi: 10.1016/j.bcp.2014.09.015

    Figure Lengend Snippet: Effect of DAMGO, loperamide and eluxadoline on G-protein activation. (A–C) Membranes (10 μg) from spinal cords of WT, μOR −/− and δOR −/− mice were subjected to a [ 35 S]GTPγS binding assay using DAMGO (A), loperamide (B), and eluxadoline (C) (0–10 μM final concentration) as described in Section 2. (D–G) Membranes (20 μg) from the ileum of WT mice were subjected to a [ 35 S]GTPγS binding assay using DAMGO (D and G), loperamide (E and G), and eluxadoline (F and G) (0–10 μM final concentration) in the presence or absence of TIPPψ (10 nM final concentration) as described in Section 2. (G) Represents E max (% of basal) obtained with 10 μM final concentration of DAMGO (±10 nM final concentration of TIPPψ), eluxadoline or loperamide. Basal values determined in the absence of the agonist were taken as 100%. Results are the mean ± S.E.M. n = 3–9. n.d., Not determined. * p

    Article Snippet: Membranes (10 or 20 μg) were subjected to a [35 S]GTPγS binding assay using DAMGO (R & D Systems, Minneapolis, USA), loperamide (Toronto Research Chemicals Inc., Ontario, Canada), eluxadoline (Furiex, Morrisville, NC, USA) (0–10 μM final concentration) in the presence or absence of TIPPψ (10 nM final concentration) (a gift from Dr. Peter Schiller, Institut de Reserches Cliniques de Montreal, Montreal, ON, Canada) as described previously [ ].

    Techniques: Activation Assay, Mouse Assay, GTPγS Binding Assay, Concentration Assay

    Effect of DAMGO, loperamide and eluxadoline on β-arrestin recruitment. Cells (5000/well) expressing either μ βgal OR (A–C) or μ βgal OR-δOR (D–F) were treated with either DAMGO (A and D), loperamide (B and E), eluxadoline (C and F) (0–10 μM final concentration) in the absence or presence of the δOR antagonist, TIPPψ (10 nM final concentration) for 60 min at 37 °C and β-arrestin recruitment was measured as described in Section 2. Results are the mean ± S.E.M. n = 4–12. * p

    Journal: Biochemical pharmacology

    Article Title: Molecular characterization of eluxadoline as a potential ligand targeting mu-delta opioid receptor heteromers

    doi: 10.1016/j.bcp.2014.09.015

    Figure Lengend Snippet: Effect of DAMGO, loperamide and eluxadoline on β-arrestin recruitment. Cells (5000/well) expressing either μ βgal OR (A–C) or μ βgal OR-δOR (D–F) were treated with either DAMGO (A and D), loperamide (B and E), eluxadoline (C and F) (0–10 μM final concentration) in the absence or presence of the δOR antagonist, TIPPψ (10 nM final concentration) for 60 min at 37 °C and β-arrestin recruitment was measured as described in Section 2. Results are the mean ± S.E.M. n = 4–12. * p

    Article Snippet: Membranes (10 or 20 μg) were subjected to a [35 S]GTPγS binding assay using DAMGO (R & D Systems, Minneapolis, USA), loperamide (Toronto Research Chemicals Inc., Ontario, Canada), eluxadoline (Furiex, Morrisville, NC, USA) (0–10 μM final concentration) in the presence or absence of TIPPψ (10 nM final concentration) (a gift from Dr. Peter Schiller, Institut de Reserches Cliniques de Montreal, Montreal, ON, Canada) as described previously [ ].

    Techniques: Expressing, Concentration Assay

    Endothelin effects on channel mutants A , diagrams depict channel mutants: Kir3.4 (S143T) parent, N-terminal truncation, and chimeras. We produced the Kir3.4(S143T) using a cDNA template for cRNA coding a Kir3.4(S143T) having a truncated (1–57) N terminus. We used Kir3 chimeras: Kir3.4(S143T)-(1–338)/Kir3.1-(333–501) and Kir3.4(S143T)-(1–249)/Kir3.1-(244–501). B , oocytes were injected with 1 ng of MOR and 1 ng of HETAR, and either 1 ng of Kir3.4(S143T), 1 ng of Kir3.4(S143T)-(Δ1–57), 1 ng of Kir3.4(S143T)-(1–338)/Kir3.1-(333–501), or 1 ng of Kir3.4(S143T)-(1–249)/Kir3.1-(244–501). The percent inhibition of the second mu opioid response after Et-1 pretreatment is shown. The bar graph summarizes the effects of Et-1 on the DAMGO activation of MOR compared with untreated controls from the same batch. Data are means ± S.E. from four to seven oocytes and two to three independent experiments.

    Journal: The Journal of biological chemistry

    Article Title: Eicosanoids Inhibit the G-protein-gated Inwardly Rectifying Potassium Channel (Kir3) at the Na+/PIP2 Gating Site *

    doi: 10.1074/jbc.M010097200

    Figure Lengend Snippet: Endothelin effects on channel mutants A , diagrams depict channel mutants: Kir3.4 (S143T) parent, N-terminal truncation, and chimeras. We produced the Kir3.4(S143T) using a cDNA template for cRNA coding a Kir3.4(S143T) having a truncated (1–57) N terminus. We used Kir3 chimeras: Kir3.4(S143T)-(1–338)/Kir3.1-(333–501) and Kir3.4(S143T)-(1–249)/Kir3.1-(244–501). B , oocytes were injected with 1 ng of MOR and 1 ng of HETAR, and either 1 ng of Kir3.4(S143T), 1 ng of Kir3.4(S143T)-(Δ1–57), 1 ng of Kir3.4(S143T)-(1–338)/Kir3.1-(333–501), or 1 ng of Kir3.4(S143T)-(1–249)/Kir3.1-(244–501). The percent inhibition of the second mu opioid response after Et-1 pretreatment is shown. The bar graph summarizes the effects of Et-1 on the DAMGO activation of MOR compared with untreated controls from the same batch. Data are means ± S.E. from four to seven oocytes and two to three independent experiments.

    Article Snippet: Endothelin-1 and DAMGO were obtained from Phoenix Pharmaceuticals, Belmont, CA and were stored at −20 °C until use.

    Techniques: Produced, Injection, Inhibition, Activation Assay

    Opioid receptor antagonist and agonist modulation MOR- or DOR-specific ligand binding in NK cells. Shown are representative saturation curves ( A, C, E, and G ) and the B max ( B, D, F, and H ) of MOR-specific ligand [ 3 H]DAMGO or DOR-specific ligand [ 3 H]naltrindole.

    Journal: The Journal of Biological Chemistry

    Article Title: Opiate Antagonist Prevents ?- and ?-Opiate Receptor Dimerization to Facilitate Ability of Agonist to Control Ethanol-altered Natural Killer Cell Functions and Mammary Tumor Growth *

    doi: 10.1074/jbc.M112.347583

    Figure Lengend Snippet: Opioid receptor antagonist and agonist modulation MOR- or DOR-specific ligand binding in NK cells. Shown are representative saturation curves ( A, C, E, and G ) and the B max ( B, D, F, and H ) of MOR-specific ligand [ 3 H]DAMGO or DOR-specific ligand [ 3 H]naltrindole.

    Article Snippet: Spleens of experimental animals were dissociated, and erythrocytes were removed by a 5-s hypotonic shock with sterile distilled water, and splenocytes were isolated and incubated at a concentration of 1–4 × 106 /well with various concentrations of [3 H]naltrindole or [3 H]DAMGO (PerkinElmer Life Sciences) in 96-well plates for 4.5 h at room temperature in Ca2+ - and Mg2+ -deficient Hanks' balanced salt solution.

    Techniques: Ligand Binding Assay

    Relative efficacy values for DAMGO, etorphine, endomorphin-2, and morphine for five MOPr signaling outputs. Values refer to efficacy relative to that of DAMGO, which was set to 1.00 for each output. The values for [ 35 S]GTPγS binding and arrestin-3

    Journal: Molecular Pharmacology

    Article Title: Endomorphin-2: A Biased Agonist at the ?-Opioid Receptor

    doi: 10.1124/mol.112.078659

    Figure Lengend Snippet: Relative efficacy values for DAMGO, etorphine, endomorphin-2, and morphine for five MOPr signaling outputs. Values refer to efficacy relative to that of DAMGO, which was set to 1.00 for each output. The values for [ 35 S]GTPγS binding and arrestin-3

    Article Snippet: Morphine hydrochloride was obtained from Mcfarlan Smith (Edinburgh, UK), etorphine from Research Triangle Institute (Research Triangle Park, NC), and DAMGO from Bachem (Bubendorf, Switzerland).

    Techniques: Binding Assay

    Concentration-response curves for activation of the GIRK current in rat LC neurons by DAMGO, etorphine, and endomorphin-2. In individual LC neurons, concentration-response curves for DAMGO ( n = 3–5) (A), etorphine ( n = 3–8) (B),and endomorphin-2

    Journal: Molecular Pharmacology

    Article Title: Endomorphin-2: A Biased Agonist at the ?-Opioid Receptor

    doi: 10.1124/mol.112.078659

    Figure Lengend Snippet: Concentration-response curves for activation of the GIRK current in rat LC neurons by DAMGO, etorphine, and endomorphin-2. In individual LC neurons, concentration-response curves for DAMGO ( n = 3–5) (A), etorphine ( n = 3–8) (B),and endomorphin-2

    Article Snippet: Morphine hydrochloride was obtained from Mcfarlan Smith (Edinburgh, UK), etorphine from Research Triangle Institute (Research Triangle Park, NC), and DAMGO from Bachem (Bubendorf, Switzerland).

    Techniques: Concentration Assay, Activation Assay

    Activation of PKC promotes a selective MOR phosphorylation at T370. (A) HEK239 cells stably expressing MOR were either not exposed (−) or exposed to 1 nM, 10 nM, 100 nM, 1 μM or 10 μM PMA for 30 min. Cells were lysed and immunoblotted with anti-pS363, anti-pT370, anti-pS375, anti-pT376 and anti-pT379 antibodies. Blots were stripped and reprobed with the phosphorylation-independent anti-MOR antibody {UMB-3} to confirm equal loading of the gel. Note that PMA stimulates a dose-dependent phosphorylation of T370. S363 is constitutively phosphorylated. (B) Cells were stably exposed to 10 μM DAMGO or 1 μM PMA for 0, 2, 5, 10, 20 or 30 min, and then immunoblotted with anti-pS375 or anti-pT370 antibodies. Blots were stripped and reprobed with the phosphorylation-independent anti-MOR antibody {UMB-3}. Representative results from one of four independent experiments per condition are shown. The position of molecular mass markers is indicated on the left (in kDa). (C) MOR-and T370A-expressing HEK293 cells were either not exposed (−) or exposed to 10 μM DAMGO or 1 μM PMA for 30 min. Cells were then stained with anti-HA antibody, processed for immunofluorescence and examined by confocal microscopy. Note that PMA induces internalization of wild-type MOR but not of the T370A mutant. Representative images from one of three independent experiments performed in duplicate are shown. Scale bar: 20 μm.

    Journal: British Journal of Pharmacology

    Article Title: Heterologous regulation of agonist-independent μ-opioid receptor phosphorylation by protein kinase C

    doi: 10.1111/bph.12546

    Figure Lengend Snippet: Activation of PKC promotes a selective MOR phosphorylation at T370. (A) HEK239 cells stably expressing MOR were either not exposed (−) or exposed to 1 nM, 10 nM, 100 nM, 1 μM or 10 μM PMA for 30 min. Cells were lysed and immunoblotted with anti-pS363, anti-pT370, anti-pS375, anti-pT376 and anti-pT379 antibodies. Blots were stripped and reprobed with the phosphorylation-independent anti-MOR antibody {UMB-3} to confirm equal loading of the gel. Note that PMA stimulates a dose-dependent phosphorylation of T370. S363 is constitutively phosphorylated. (B) Cells were stably exposed to 10 μM DAMGO or 1 μM PMA for 0, 2, 5, 10, 20 or 30 min, and then immunoblotted with anti-pS375 or anti-pT370 antibodies. Blots were stripped and reprobed with the phosphorylation-independent anti-MOR antibody {UMB-3}. Representative results from one of four independent experiments per condition are shown. The position of molecular mass markers is indicated on the left (in kDa). (C) MOR-and T370A-expressing HEK293 cells were either not exposed (−) or exposed to 10 μM DAMGO or 1 μM PMA for 30 min. Cells were then stained with anti-HA antibody, processed for immunofluorescence and examined by confocal microscopy. Note that PMA induces internalization of wild-type MOR but not of the T370A mutant. Representative images from one of three independent experiments performed in duplicate are shown. Scale bar: 20 μm.

    Article Snippet: The following MOR agonists were obtained from commercial suppliers: morphine (Merck Pharma, Darmstadt, Germany), DAMGO (Sanofi-Aventis, Frankfurt, Germany) and etonitazene (Novartis, Basel, Switzerland).

    Techniques: Activation Assay, Stable Transfection, Expressing, Staining, Immunofluorescence, Confocal Microscopy, Mutagenesis