Journal: Nature Communications
Article Title: Alcohol exposure disrupts mu opioid receptor-mediated long-term depression at insular cortex inputs to dorsolateral striatum
Figure Lengend Snippet: Anterior insular MORs are necessary to induce mOP-LTD in the DLS. a Schematic figure of coronal brain slice showing the recording of EPSCs evoked by focal electric stimulation in the DLS of MOR-flox mice. An AAV vector encoding for cre-recombinase (AAV.hSyn.cre) was injected 8 weeks prior to recordings. Coronal brain slice showing the infection of anterior insular cortex and dorsal striatal terminal expression (bar scale = 1000 μm). b Representative electrically evoked synaptic traces and a recording time course from the DLS before and after DAMGO (0.3 μM, 5 min) showing the loss of mOP-LTD mediated by anterior insular MORs ( P = 0.136, t 5 = 1.775, n = 6 from 4 mice). c , d Representative electrically evoked synaptic traces and a recording time course from the DLS before and after DAMGO (0.3 μM, 5 min) and high-frequency stimulation (HFS) coupled with depolarization (4 pulses of 100 Hz, 10 s inter-pulse interval). DAMGO application was incapable of producing mOP-LTD in the DLS of anterior insula MOR knockout mice. However, HFS induced a strong reduction of eEPSCs in the DLS, indicating intact eCB-LTD (baseline vs DAMGO: P = 0.262, t 3 = 1.3.78; baseline vs HFS: P = 0.0082, t 3 = 6.259; DAMGO vs HFS: P = 0.0467, t 3 = 3.273; n = 4 from 3 mice). Data analyzed with Student’s paired t tests. Data represent mean ± SEM
Article Snippet: Reagents The MOR agonist DAMGO (H-2535, Bachem), MOR antagonist CTAP (H-3698, Bachem), delta opioid receptor agonist [D-Pen2 ,D-Pen5 ]-enkephalin (DPDPE; H-2905m, Bachem), α7 nicotinic acetylcholine receptor antagonist methyllycaconitine citrate (MLA; 1029, Tocris), AMPA antagonist NBQX (0373, Tocris), tetrodotoxin citrate (ARCD-0640, ARC Inc.), EtOH (E7148, Sigma-Aldrich), and GABAA receptor antagonist picrotoxin (P1675, Sigma-Aldrich) were used.
Techniques: Slice Preparation, Mouse Assay, Plasmid Preparation, Injection, Infection, Expressing, Knock-Out