Journal: iScience

Article Title: Thrombospondin1 mimics rapidly relieve depression via Shank3 dependent uncoupling between dopamine D1 and D2 receptors

doi: 10.1016/j.isci.2023.106488

Figure Lengend Snippet: Key resources table

Article Snippet: D1R , Sigma-Aldrich , Cat# D2944, RRID: AB_1840787.

Techniques: Recombinant, Magnetic Beads, Real-time Polymerase Chain Reaction, Software

Journal: Molecular Pain

Article Title: Expression of the dopaminergic D1 and D2 receptors in the anterior cingulate cortex in a model of neuropathic pain

doi: 10.1186/1744-8069-7-97

Figure Lengend Snippet: A. Semiquantitative mRNA levels for D1R in cg1 . The graph depicts a decrease in all groups when compared to control. Low, medium and high AS groups show 92 ± 2.4, 52 ± 1.3 and 62 ± 0.6%, respectively considering the control group as 100%. The lowest mRNA level is in the medium AS group. Image B shows mRNA levels for D2R in the same region with 79 ± 1.2, 120 ± 1.5 and 129 ± 1.6% for groups with low, medium and high AS, respectively. Notice that there is a progressive increase in mRNA levels, as the autotomy behaviour increases (n = 4 in all groups). All percentages are significantly different when compared to control (one-way ANOVA, Bonferroni post hoc *p < 0.05). At the top of each frame, there is an example of the electrophoresis agarose gel showing the control oligonucleotide (upper band) and the cDNA of D1R's in (A) and D2R's in (B) (lower band).

Article Snippet: Then, the membranes were incubated in the primary Ab Goat anti D1R (Santa Cruz, cat. sc-1434) dil. 1:5000 in the same solution at 4 ° C overnight with stirring CTE.

Techniques: Electrophoresis, Agarose Gel Electrophoresis

Journal: Molecular Pain

Article Title: Expression of the dopaminergic D1 and D2 receptors in the anterior cingulate cortex in a model of neuropathic pain

doi: 10.1186/1744-8069-7-97

Figure Lengend Snippet: A. This figure shows semiquantitative mRNA levels for D1R in cg2 . Notice an increase in all groups when compared to control. Low, medium and high AS groups show 121 ± 1.1, 269 ± 3.3 and 207 ± 1%, respectively considering the control group as 100%. The greatest increase is in the medium AS group. Image B shows mRNA levels for D2R in the same region with 71 ± 2.6, 44 ± 1.8 and 71 ± 0.8% for groups with low, medium and high AS, respectively. Also, notice that the lowest value is for the medium AS group (n = 4 in all groups). All percentages are significantly different when compared to control (one-way ANOVA, Bonferroni post hoc *p < 0.05). At the top of each frame, there is an example of the electrophoresis agarose gel showing the control oligonucleotide (upper band) and the cDNA of D1R's in (A) and D2R's in (B) (lower band).

Article Snippet: Then, the membranes were incubated in the primary Ab Goat anti D1R (Santa Cruz, cat. sc-1434) dil. 1:5000 in the same solution at 4 ° C overnight with stirring CTE.

Techniques: Electrophoresis, Agarose Gel Electrophoresis

Journal: Molecular Pain

Article Title: Expression of the dopaminergic D1 and D2 receptors in the anterior cingulate cortex in a model of neuropathic pain

doi: 10.1186/1744-8069-7-97

Figure Lengend Snippet: Immuno Dot performed for D1R in control animals in the cerebral cortex . This control was done in order to validate the experimental procedure for dopamine D1 receptors in the ACC and the specificity of the antibody. The (A) column shows the blocking D1R peptide without primary antibody, (B) shows the blocking D1R peptide with D1R antibody and (C) the result in the cerebral cortex of control animals. The concentrations shown are the ones with positivity; the results were negative from 10 -3 .

Article Snippet: Then, the membranes were incubated in the primary Ab Goat anti D1R (Santa Cruz, cat. sc-1434) dil. 1:5000 in the same solution at 4 ° C overnight with stirring CTE.

Techniques: Blocking Assay