Journal: Frontiers in Cardiovascular Medicine

Article Title: Loss of Function in Dopamine D3 Receptor Attenuates Left Ventricular Cardiac Fibroblast Migration and Proliferation in vitro

doi: 10.3389/fcvm.2021.732282

Figure Lengend Snippet: The expression and distribution of dopamine receptors, D1R and D3R in mouse hearts: (A) Representative photomicrographs showing immunofluorescence staining of D1R and D3R which co-localized with vimentin, a cardiac fibroblast-specific marker, in the heart tissue sections. No primary antibody control sections were incubated with the antibody diluent alone and no primary antibody, followed by incubation with secondary antibodies and DAPI (the nuclear stain), which confirms the antibody specificity of D1R and D3R. (B) Gene expression was determined using TaqMan primers by real-time qPCR. The normalized -dCT values indicate that there is relatively higher expression of D1R compared to D3R in cardiac tissue. (C) Receptor protein expression determined by western blot using receptor specific antibodies as described in methods section.

Article Snippet: After electrophoresis, proteins were transferred and immunoblotting was performed for dopamine receptors: D1R (1:500, ab78021 Abcam); and D3R (1:5000, ab42114 Abcam) ( ).

Techniques: Expressing, Immunofluorescence, Staining, Marker, Incubation, Western Blot

Journal: Frontiers in Cardiovascular Medicine

Article Title: Loss of Function in Dopamine D3 Receptor Attenuates Left Ventricular Cardiac Fibroblast Migration and Proliferation in vitro

doi: 10.3389/fcvm.2021.732282

Figure Lengend Snippet: The expression and distribution of dopamine receptors, D1R and D3R in mouse primary cardiac fibroblasts: (A) Representative photomicrographs showing immunofluorescence staining of D1R and D3R which co-localized with cardiac fibroblasts specific marker vimentin staining in the isolated WT and D3KO primary cardiac fibroblasts in culture. Cardiac fibroblast expression of D1R and D3R is not lost when primary cells are maintained in cell culture. As expected, there is no observable staining for D3R in D3KO cardiac fibroblasts. No primary antibody control images show the specificity of the primary antibodies used. (B) Gene expression determined using TaqMan primers by real-time qPCR. The normalized -dCT values indicate that there is relatively higher expression D1R compared to D3R in cardiac fibroblasts. (C) Receptor protein expression determined by western blot using receptor specific antibodies as described in methods section.

Article Snippet: After electrophoresis, proteins were transferred and immunoblotting was performed for dopamine receptors: D1R (1:500, ab78021 Abcam); and D3R (1:5000, ab42114 Abcam) ( ).

Techniques: Expressing, Immunofluorescence, Staining, Marker, Isolation, Cell Culture, Western Blot