Journal: Neurology® Neuroimmunology & Neuroinflammation
Article Title: Varicella zoster virus–infected cerebrovascular cells produce a proinflammatory environment
Figure Lengend Snippet: HBVAFs secrete the highest level of cytokines among vascular and lung cells Cell culture supernatants from mock-infected HBVAFs, HPNCs, HBVSMCs, and HFLs were harvested at 72 hpi and analyzed for IL-1β, IL-1α, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12p70, IL-12/23p40, IL-13, IL-15, IL-16, IL-17a, IFNγ, TNF-α, TNF-β, Eotaxin-1, Eotaxin-3, IP-10, MCP-1, MCP-4, MDC, MIP-1α, MIP-1β, TARC, GM-CSF, TGF-β, and VEGF-A levels. Only significant fold-change in HBVAFs cytokine levels compared with HPNCs (red), HBVSMCs (blue), or HFLs (green) were plotted. Bar graphs represent mean ± SEM fold-change in cytokine levels from triplicate experiments. (a) p
Article Snippet: Conditioned supernatant from mock- and VZV-infected human brain vascular adventitial fibroblasts (HBVAFs), human perineurial cells (HPNCs), human brain vascular smooth muscle cells (HBVSMCs), and human fetal lung fibroblasts (HFLs) were collected at 72 hours postinfection and analyzed for levels of 30 proinflammatory cytokines using the Meso Scale Discovery Multiplex ELISA platform.
Techniques: Cell Culture, Infection