cytokeratin antibody Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Thermo Fisher monoclonal anti rat keratin
    Monoclonal Anti Rat Keratin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal anti rat keratin/product/Thermo Fisher
    Average 93 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    monoclonal anti rat keratin - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    94
    Millipore cytokeratin antibodies
    Capture of SK-BR-3 and T47D cells with the cmHsp70.1 mAb-functionalized CellCollector® system. (A) SK-BR-3 cells were stained with CFSE (green) and incubated with the cmHsp70.1 mAb-functionalized CellCollector® system. Captured cells were analyzed with a fluorescence microscope. Scale bar 100 μm. (B) SK-BR-3 or T47D cells were incubated with the cmHsp70.1 mAb-functionalized CellCollector® system for 30 min, fixed and stained with <t>cytokeratin</t> antibodies (CK-FITC, green) and Hoechst 33342 (DAPI, blue). Scale bar 25 μm.
    Cytokeratin Antibodies, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cytokeratin antibodies/product/Millipore
    Average 94 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    cytokeratin antibodies - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    94
    Cell Signaling Technology Inc cytokeratin antibody
    p95HER2 immunofluorescence. Immunofluorescence assay for detection of p95HER2 (top = p95HER2 negative case, bottom = p95HER2+ case). HER2 intracellular domain staining (red) colocalizes with <t>cytokeratin</t> staining (green) only in p95HER2+ tumors. Colocalization appears as a yellow (red and green merged) signal.
    Cytokeratin Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cytokeratin antibody/product/Cell Signaling Technology Inc
    Average 94 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    cytokeratin antibody - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    94
    Abcam anti keratin
    p95HER2 immunofluorescence. Immunofluorescence assay for detection of p95HER2 (top = p95HER2 negative case, bottom = p95HER2+ case). HER2 intracellular domain staining (red) colocalizes with <t>cytokeratin</t> staining (green) only in p95HER2+ tumors. Colocalization appears as a yellow (red and green merged) signal.
    Anti Keratin, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti keratin/product/Abcam
    Average 94 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    anti keratin - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    94
    Abcam anti keratin 36 antibody
    p95HER2 immunofluorescence. Immunofluorescence assay for detection of p95HER2 (top = p95HER2 negative case, bottom = p95HER2+ case). HER2 intracellular domain staining (red) colocalizes with <t>cytokeratin</t> staining (green) only in p95HER2+ tumors. Colocalization appears as a yellow (red and green merged) signal.
    Anti Keratin 36 Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti keratin 36 antibody/product/Abcam
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti keratin 36 antibody - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    93
    Thermo Fisher cytokeratin
    Plantar incision induced the TNFα expression in keratinocytes in margin incision skin in both C57BL/6J and KK mice. Representative confocal images of <t>cytokeratin</t> (green) and TNFα (red) immunoreactivity in the margin incision skin. Colocalization (merge) is shown in yellow/orange. The left columns of both groups are shown in lower magnification. The right columns of both groups are shown in higher magnification. Scale bars: 50 μm (lower magnification) and 20 μm (higher magnification). In C57BL/6J mice, the co-expression of cytokeratin and TNFα began to increase at one day after incision ( P
    Cytokeratin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 395 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cytokeratin/product/Thermo Fisher
    Average 93 stars, based on 395 article reviews
    Price from $9.99 to $1999.99
    cytokeratin - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    86
    Agilent technologies cyto keratin antibody
    <t>Cytokeratin</t> expression in ovine OSE. Immunohistochemistry for (A) intact OSE cells ( in vivo ) and (B) isolated OSE cells ( in vitro ), at 600× magnification.
    Cyto Keratin Antibody, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cyto keratin antibody/product/Agilent technologies
    Average 86 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    cyto keratin antibody - by Bioz Stars, 2020-08
    86/100 stars
      Buy from Supplier

    94
    Boster Bio keratin monoclonal antibodies
    <t>Cytokeratin</t> expression in ovine OSE. Immunohistochemistry for (A) intact OSE cells ( in vivo ) and (B) isolated OSE cells ( in vitro ), at 600× magnification.
    Keratin Monoclonal Antibodies, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/keratin monoclonal antibodies/product/Boster Bio
    Average 94 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    keratin monoclonal antibodies - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    85
    Covance keratin 10 antibody
    Abnormal eyelid epithelial differentiation in Fgfr2 CKO and Smad4 CKO mice. ( A-C ) Immunostaining for keratin 14 (K14) in frontal sections from wild-type (A), Fgfr2 CKO (B) and Smad4 CKO (C) embryos at E15.0. In wild-type eyelids (A), K14 staining was weak in the palpebral conjunctiva and was not detected in the bulbar conjunctiva (arrows). In Fgfr2 CKO (B) and Smad4 CKO (C) eyelids, K14 staining was strong and uniform in the palpebral and bulbar conjunctiva (arrows). ( D-F ) Immunostaining for <t>keratin</t> 10 (K10) in frontal sections from wild type (D), Fgfr2 CKO (E) and Smad4 CKO (F) embryos at E17.5. In wild-type eyelids (D), K10 staining is observed only in the epidermis; it is negative in the conjunctiva (arrow). In Fgfr2 CKO (E), K10 is found in epidermis and a few cells of the palpebral conjunctiva, although the majority of conjunctival cells do not express K10 (arrow). In Smad4 CKO (F), K10 is detected in the epidermis and in the majority of conjunctival cells (arrow). ( G-I ) Immunostaining for keratin 4 (K4) in frontal sections from wild-type (G), Fgfr2 CKO (H) and Smad4 CKO (I) embryos at E17.5. In wild-type eyelids (G), expression of K4 is continuous in the conjunctiva (arrow). In Fgfr2 CKO (H), K4 is found in most conjunctival cells (arrow). In Smad4 CKO (I), K4 is only detected in a few conjunctival cells; most conjunctival cells do not express K4 (arrow). Scale bars: 100 μm.
    Keratin 10 Antibody, supplied by Covance, used in various techniques. Bioz Stars score: 85/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/keratin 10 antibody/product/Covance
    Average 85 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    keratin 10 antibody - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    90
    Nichirei keratin cytokeratin
    Abnormal eyelid epithelial differentiation in Fgfr2 CKO and Smad4 CKO mice. ( A-C ) Immunostaining for keratin 14 (K14) in frontal sections from wild-type (A), Fgfr2 CKO (B) and Smad4 CKO (C) embryos at E15.0. In wild-type eyelids (A), K14 staining was weak in the palpebral conjunctiva and was not detected in the bulbar conjunctiva (arrows). In Fgfr2 CKO (B) and Smad4 CKO (C) eyelids, K14 staining was strong and uniform in the palpebral and bulbar conjunctiva (arrows). ( D-F ) Immunostaining for <t>keratin</t> 10 (K10) in frontal sections from wild type (D), Fgfr2 CKO (E) and Smad4 CKO (F) embryos at E17.5. In wild-type eyelids (D), K10 staining is observed only in the epidermis; it is negative in the conjunctiva (arrow). In Fgfr2 CKO (E), K10 is found in epidermis and a few cells of the palpebral conjunctiva, although the majority of conjunctival cells do not express K10 (arrow). In Smad4 CKO (F), K10 is detected in the epidermis and in the majority of conjunctival cells (arrow). ( G-I ) Immunostaining for keratin 4 (K4) in frontal sections from wild-type (G), Fgfr2 CKO (H) and Smad4 CKO (I) embryos at E17.5. In wild-type eyelids (G), expression of K4 is continuous in the conjunctiva (arrow). In Fgfr2 CKO (H), K4 is found in most conjunctival cells (arrow). In Smad4 CKO (I), K4 is only detected in a few conjunctival cells; most conjunctival cells do not express K4 (arrow). Scale bars: 100 μm.
    Keratin Cytokeratin, supplied by Nichirei, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/keratin cytokeratin/product/Nichirei
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    keratin cytokeratin - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

    85
    Agilent technologies anti keratin antibody
    Abnormal eyelid epithelial differentiation in Fgfr2 CKO and Smad4 CKO mice. ( A-C ) Immunostaining for keratin 14 (K14) in frontal sections from wild-type (A), Fgfr2 CKO (B) and Smad4 CKO (C) embryos at E15.0. In wild-type eyelids (A), K14 staining was weak in the palpebral conjunctiva and was not detected in the bulbar conjunctiva (arrows). In Fgfr2 CKO (B) and Smad4 CKO (C) eyelids, K14 staining was strong and uniform in the palpebral and bulbar conjunctiva (arrows). ( D-F ) Immunostaining for <t>keratin</t> 10 (K10) in frontal sections from wild type (D), Fgfr2 CKO (E) and Smad4 CKO (F) embryos at E17.5. In wild-type eyelids (D), K10 staining is observed only in the epidermis; it is negative in the conjunctiva (arrow). In Fgfr2 CKO (E), K10 is found in epidermis and a few cells of the palpebral conjunctiva, although the majority of conjunctival cells do not express K10 (arrow). In Smad4 CKO (F), K10 is detected in the epidermis and in the majority of conjunctival cells (arrow). ( G-I ) Immunostaining for keratin 4 (K4) in frontal sections from wild-type (G), Fgfr2 CKO (H) and Smad4 CKO (I) embryos at E17.5. In wild-type eyelids (G), expression of K4 is continuous in the conjunctiva (arrow). In Fgfr2 CKO (H), K4 is found in most conjunctival cells (arrow). In Smad4 CKO (I), K4 is only detected in a few conjunctival cells; most conjunctival cells do not express K4 (arrow). Scale bars: 100 μm.
    Anti Keratin Antibody, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti keratin antibody/product/Agilent technologies
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    anti keratin antibody - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    89
    Immunotec anti keratin
    Abnormal eyelid epithelial differentiation in Fgfr2 CKO and Smad4 CKO mice. ( A-C ) Immunostaining for keratin 14 (K14) in frontal sections from wild-type (A), Fgfr2 CKO (B) and Smad4 CKO (C) embryos at E15.0. In wild-type eyelids (A), K14 staining was weak in the palpebral conjunctiva and was not detected in the bulbar conjunctiva (arrows). In Fgfr2 CKO (B) and Smad4 CKO (C) eyelids, K14 staining was strong and uniform in the palpebral and bulbar conjunctiva (arrows). ( D-F ) Immunostaining for <t>keratin</t> 10 (K10) in frontal sections from wild type (D), Fgfr2 CKO (E) and Smad4 CKO (F) embryos at E17.5. In wild-type eyelids (D), K10 staining is observed only in the epidermis; it is negative in the conjunctiva (arrow). In Fgfr2 CKO (E), K10 is found in epidermis and a few cells of the palpebral conjunctiva, although the majority of conjunctival cells do not express K10 (arrow). In Smad4 CKO (F), K10 is detected in the epidermis and in the majority of conjunctival cells (arrow). ( G-I ) Immunostaining for keratin 4 (K4) in frontal sections from wild-type (G), Fgfr2 CKO (H) and Smad4 CKO (I) embryos at E17.5. In wild-type eyelids (G), expression of K4 is continuous in the conjunctiva (arrow). In Fgfr2 CKO (H), K4 is found in most conjunctival cells (arrow). In Smad4 CKO (I), K4 is only detected in a few conjunctival cells; most conjunctival cells do not express K4 (arrow). Scale bars: 100 μm.
    Anti Keratin, supplied by Immunotec, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti keratin/product/Immunotec
    Average 89 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti keratin - by Bioz Stars, 2020-08
    89/100 stars
      Buy from Supplier

    85
    Covance antibodies against keratins k1
    Abnormal eyelid epithelial differentiation in Fgfr2 CKO and Smad4 CKO mice. ( A-C ) Immunostaining for keratin 14 (K14) in frontal sections from wild-type (A), Fgfr2 CKO (B) and Smad4 CKO (C) embryos at E15.0. In wild-type eyelids (A), K14 staining was weak in the palpebral conjunctiva and was not detected in the bulbar conjunctiva (arrows). In Fgfr2 CKO (B) and Smad4 CKO (C) eyelids, K14 staining was strong and uniform in the palpebral and bulbar conjunctiva (arrows). ( D-F ) Immunostaining for <t>keratin</t> 10 (K10) in frontal sections from wild type (D), Fgfr2 CKO (E) and Smad4 CKO (F) embryos at E17.5. In wild-type eyelids (D), K10 staining is observed only in the epidermis; it is negative in the conjunctiva (arrow). In Fgfr2 CKO (E), K10 is found in epidermis and a few cells of the palpebral conjunctiva, although the majority of conjunctival cells do not express K10 (arrow). In Smad4 CKO (F), K10 is detected in the epidermis and in the majority of conjunctival cells (arrow). ( G-I ) Immunostaining for keratin 4 (K4) in frontal sections from wild-type (G), Fgfr2 CKO (H) and Smad4 CKO (I) embryos at E17.5. In wild-type eyelids (G), expression of K4 is continuous in the conjunctiva (arrow). In Fgfr2 CKO (H), K4 is found in most conjunctival cells (arrow). In Smad4 CKO (I), K4 is only detected in a few conjunctival cells; most conjunctival cells do not express K4 (arrow). Scale bars: 100 μm.
    Antibodies Against Keratins K1, supplied by Covance, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies against keratins k1/product/Covance
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    antibodies against keratins k1 - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    89
    Santa Cruz Biotechnology anti keratin
    Abnormal eyelid epithelial differentiation in Fgfr2 CKO and Smad4 CKO mice. ( A-C ) Immunostaining for keratin 14 (K14) in frontal sections from wild-type (A), Fgfr2 CKO (B) and Smad4 CKO (C) embryos at E15.0. In wild-type eyelids (A), K14 staining was weak in the palpebral conjunctiva and was not detected in the bulbar conjunctiva (arrows). In Fgfr2 CKO (B) and Smad4 CKO (C) eyelids, K14 staining was strong and uniform in the palpebral and bulbar conjunctiva (arrows). ( D-F ) Immunostaining for <t>keratin</t> 10 (K10) in frontal sections from wild type (D), Fgfr2 CKO (E) and Smad4 CKO (F) embryos at E17.5. In wild-type eyelids (D), K10 staining is observed only in the epidermis; it is negative in the conjunctiva (arrow). In Fgfr2 CKO (E), K10 is found in epidermis and a few cells of the palpebral conjunctiva, although the majority of conjunctival cells do not express K10 (arrow). In Smad4 CKO (F), K10 is detected in the epidermis and in the majority of conjunctival cells (arrow). ( G-I ) Immunostaining for keratin 4 (K4) in frontal sections from wild-type (G), Fgfr2 CKO (H) and Smad4 CKO (I) embryos at E17.5. In wild-type eyelids (G), expression of K4 is continuous in the conjunctiva (arrow). In Fgfr2 CKO (H), K4 is found in most conjunctival cells (arrow). In Smad4 CKO (I), K4 is only detected in a few conjunctival cells; most conjunctival cells do not express K4 (arrow). Scale bars: 100 μm.
    Anti Keratin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 89/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti keratin/product/Santa Cruz Biotechnology
    Average 89 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    anti keratin - by Bioz Stars, 2020-08
    89/100 stars
      Buy from Supplier

    93
    Millipore anti keratin 10
    SIG-1191 increases AQP3 gene expression and protein levels in Reconstructed Human Epidermis (RHE). EpiDerm-FT™ air–liquid interface cultures were topically treated with 0.25–0.5% (w/v) of SIG-1191 for 24 h. a AQP3 gene expression was analyzed by qPCR normalized to GAPDH a control housekeeping gene. The data represent the mean ± SEM of a representative experiment. b Haematoxylin and eosin (H E) staining and immunohistochemistry (IHC) of EpiDerm-FT™ tissues. Immunohistochemistry was performed with anti-aquaporin-3 ( green ), <t>anti-keratin-10</t> ( red ), anti-rabbit Alexa-488, anti-mouseAlexa-594 antibodies. Sections were counterstained with 2-(4-amidinophenyl)-1H-indole-6-carboxamidine (DAPI). Merged micrograph shows overlaying K10 and AQP3 antibodies with DAPI staining. No background fluorescence was observed in the absence of the primary antibody (not shown). Original magnification: ×400
    Anti Keratin 10, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti keratin 10/product/Millipore
    Average 93 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    anti keratin 10 - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    85
    Progen Biotechnik keratin k6
    SIG-1191 increases AQP3 gene expression and protein levels in Reconstructed Human Epidermis (RHE). EpiDerm-FT™ air–liquid interface cultures were topically treated with 0.25–0.5% (w/v) of SIG-1191 for 24 h. a AQP3 gene expression was analyzed by qPCR normalized to GAPDH a control housekeeping gene. The data represent the mean ± SEM of a representative experiment. b Haematoxylin and eosin (H E) staining and immunohistochemistry (IHC) of EpiDerm-FT™ tissues. Immunohistochemistry was performed with anti-aquaporin-3 ( green ), <t>anti-keratin-10</t> ( red ), anti-rabbit Alexa-488, anti-mouseAlexa-594 antibodies. Sections were counterstained with 2-(4-amidinophenyl)-1H-indole-6-carboxamidine (DAPI). Merged micrograph shows overlaying K10 and AQP3 antibodies with DAPI staining. No background fluorescence was observed in the absence of the primary antibody (not shown). Original magnification: ×400
    Keratin K6, supplied by Progen Biotechnik, used in various techniques. Bioz Stars score: 85/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/keratin k6/product/Progen Biotechnik
    Average 85 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    keratin k6 - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    95
    Abcam cytokeratin
    Human periodontal ligament cells at passage three stained (A) positive for vimentin and (B) negative for <t>cytokeratin.</t> Blue indicates DAPI staining of nuclei (magnification ×200).
    Cytokeratin, supplied by Abcam, used in various techniques. Bioz Stars score: 95/100, based on 402 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cytokeratin/product/Abcam
    Average 95 stars, based on 402 article reviews
    Price from $9.99 to $1999.99
    cytokeratin - by Bioz Stars, 2020-08
    95/100 stars
      Buy from Supplier

    89
    Acris Antibodies anti keratin
    Human periodontal ligament cells at passage three stained (A) positive for vimentin and (B) negative for <t>cytokeratin.</t> Blue indicates DAPI staining of nuclei (magnification ×200).
    Anti Keratin, supplied by Acris Antibodies, used in various techniques. Bioz Stars score: 89/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti keratin/product/Acris Antibodies
    Average 89 stars, based on 30 article reviews
    Price from $9.99 to $1999.99
    anti keratin - by Bioz Stars, 2020-08
    89/100 stars
      Buy from Supplier

    90
    Zhongshan Golden Bridge Company anti keratin
    Human periodontal ligament cells at passage three stained (A) positive for vimentin and (B) negative for <t>cytokeratin.</t> Blue indicates DAPI staining of nuclei (magnification ×200).
    Anti Keratin, supplied by Zhongshan Golden Bridge Company, used in various techniques. Bioz Stars score: 90/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti keratin/product/Zhongshan Golden Bridge Company
    Average 90 stars, based on 28 article reviews
    Price from $9.99 to $1999.99
    anti keratin - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

    Image Search Results


    Capture of SK-BR-3 and T47D cells with the cmHsp70.1 mAb-functionalized CellCollector® system. (A) SK-BR-3 cells were stained with CFSE (green) and incubated with the cmHsp70.1 mAb-functionalized CellCollector® system. Captured cells were analyzed with a fluorescence microscope. Scale bar 100 μm. (B) SK-BR-3 or T47D cells were incubated with the cmHsp70.1 mAb-functionalized CellCollector® system for 30 min, fixed and stained with cytokeratin antibodies (CK-FITC, green) and Hoechst 33342 (DAPI, blue). Scale bar 25 μm.

    Journal: Frontiers in Oncology

    Article Title: Membrane Hsp70—A Novel Target for the Isolation of Circulating Tumor Cells After Epithelial-to-Mesenchymal Transition

    doi: 10.3389/fonc.2018.00497

    Figure Lengend Snippet: Capture of SK-BR-3 and T47D cells with the cmHsp70.1 mAb-functionalized CellCollector® system. (A) SK-BR-3 cells were stained with CFSE (green) and incubated with the cmHsp70.1 mAb-functionalized CellCollector® system. Captured cells were analyzed with a fluorescence microscope. Scale bar 100 μm. (B) SK-BR-3 or T47D cells were incubated with the cmHsp70.1 mAb-functionalized CellCollector® system for 30 min, fixed and stained with cytokeratin antibodies (CK-FITC, green) and Hoechst 33342 (DAPI, blue). Scale bar 25 μm.

    Article Snippet: Cells were stained with a cocktail of cytokeratin antibodies at RT for 30 min in the dark: CK7 (LP5K, Millipore, Billerica, USA), CK19 (A53-B/A2, Exbio, Vestec, Czech Republic), and panCK ( – , , , , ) (C11, Exbio, Vestec, Czech Republic).

    Techniques: Staining, Incubation, Fluorescence, Microscopy

    Capture of CTCs from the EDTA blood of patients using EpCAM mAb- or cmHsp70.1 mAb-functionalized magnetic beads. Representative views of singular (A) and clustered (B) CTCs derived from EDTA blood (7.5 ml, each) of patients with squamous cell carcinoma of the head and neck (SCCHN) and non-small cell lung carcinoma (NSCLC) bound to the EpCAM mAb- and cmHsp70.1 mAb-functionalized beads: brightfield, DAPI (blue), FITC-labeled cytokeratin antibody (CK-FITC, green), PE-labeled EpCAM (EpCAM-PE, red), overlay derived thereof. Scale bar 20 μm.

    Journal: Frontiers in Oncology

    Article Title: Membrane Hsp70—A Novel Target for the Isolation of Circulating Tumor Cells After Epithelial-to-Mesenchymal Transition

    doi: 10.3389/fonc.2018.00497

    Figure Lengend Snippet: Capture of CTCs from the EDTA blood of patients using EpCAM mAb- or cmHsp70.1 mAb-functionalized magnetic beads. Representative views of singular (A) and clustered (B) CTCs derived from EDTA blood (7.5 ml, each) of patients with squamous cell carcinoma of the head and neck (SCCHN) and non-small cell lung carcinoma (NSCLC) bound to the EpCAM mAb- and cmHsp70.1 mAb-functionalized beads: brightfield, DAPI (blue), FITC-labeled cytokeratin antibody (CK-FITC, green), PE-labeled EpCAM (EpCAM-PE, red), overlay derived thereof. Scale bar 20 μm.

    Article Snippet: Cells were stained with a cocktail of cytokeratin antibodies at RT for 30 min in the dark: CK7 (LP5K, Millipore, Billerica, USA), CK19 (A53-B/A2, Exbio, Vestec, Czech Republic), and panCK ( – , , , , ) (C11, Exbio, Vestec, Czech Republic).

    Techniques: Magnetic Beads, Derivative Assay, Labeling

    Percentage of cytokeratin-staining ductal cells positive for Ki67. LND, lean non-diabetic ( n = 9); OND, obese non-diabetic ( n = 11); LD, Lean with type 2 diabetes ( n = 12); OD, obese with type 2 diabetes ( n = 13); CP, chronic pancreatitis in tissue sample ( n = 7); AdCa, adenocarcinoma present in tissue sample ( n = 2). Data are presented as mean ± SEM. * p

    Journal: Diabetologia

    Article Title: Pancreatic duct replication is increased with obesity and type 2 diabetes in humans

    doi: 10.1007/s00125-009-1556-8

    Figure Lengend Snippet: Percentage of cytokeratin-staining ductal cells positive for Ki67. LND, lean non-diabetic ( n = 9); OND, obese non-diabetic ( n = 11); LD, Lean with type 2 diabetes ( n = 12); OD, obese with type 2 diabetes ( n = 13); CP, chronic pancreatitis in tissue sample ( n = 7); AdCa, adenocarcinoma present in tissue sample ( n = 2). Data are presented as mean ± SEM. * p

    Article Snippet: Sections, 4 μm, were cut from the paraffin blocks and stained for haematoxylin/eosin and double-stained for Ki67 (anti-human Ki67 monoclonal antibody MIB-1, 1/25 [Dako, Carpinteria, CA, USA]) and cytokeratin (monoclonal anti-pancytokeratin, 1/50 [Sigma-Aldrich, St Louis, MO, USA]) by immunohistochemistry.

    Techniques: Staining

    Percentage of cytokeratin-staining ductal cells positive for Ki67. LND, lean non-diabetic ( n = 9); OND, obese non-diabetic ( n = 11); LD, lean with type 2 diabetes ( n = 12); OD, obese with type 2 diabetes ( n = 13). Data are presented as mean ± SEM. * p

    Journal: Diabetologia

    Article Title: Pancreatic duct replication is increased with obesity and type 2 diabetes in humans

    doi: 10.1007/s00125-009-1556-8

    Figure Lengend Snippet: Percentage of cytokeratin-staining ductal cells positive for Ki67. LND, lean non-diabetic ( n = 9); OND, obese non-diabetic ( n = 11); LD, lean with type 2 diabetes ( n = 12); OD, obese with type 2 diabetes ( n = 13). Data are presented as mean ± SEM. * p

    Article Snippet: Sections, 4 μm, were cut from the paraffin blocks and stained for haematoxylin/eosin and double-stained for Ki67 (anti-human Ki67 monoclonal antibody MIB-1, 1/25 [Dako, Carpinteria, CA, USA]) and cytokeratin (monoclonal anti-pancytokeratin, 1/50 [Sigma-Aldrich, St Louis, MO, USA]) by immunohistochemistry.

    Techniques: Staining

    p95HER2 immunofluorescence. Immunofluorescence assay for detection of p95HER2 (top = p95HER2 negative case, bottom = p95HER2+ case). HER2 intracellular domain staining (red) colocalizes with cytokeratin staining (green) only in p95HER2+ tumors. Colocalization appears as a yellow (red and green merged) signal.

    Journal: Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer

    Article Title: p95HER2 Truncated Form in Resected Non-small Cell Lung Cancer

    doi: 10.1097/JTO.0b013e318249e13f

    Figure Lengend Snippet: p95HER2 immunofluorescence. Immunofluorescence assay for detection of p95HER2 (top = p95HER2 negative case, bottom = p95HER2+ case). HER2 intracellular domain staining (red) colocalizes with cytokeratin staining (green) only in p95HER2+ tumors. Colocalization appears as a yellow (red and green merged) signal.

    Article Snippet: After rinse with PBS, the sections were incubated with Alexa Fluor 555 antimouse IgG (Cell Signaling Technology, Beverly, MA) diluted 1:500 for 30 minutes to detect HER2 antibodies and with Alexa Fluor 488 antirabbit IgG diluted 1:500 for 30 minutes to detect cytokeratin antibody (Cell Signaling Technology).

    Techniques: Immunofluorescence, Staining

    Plantar incision induced the TNFα expression in keratinocytes in margin incision skin in both C57BL/6J and KK mice. Representative confocal images of cytokeratin (green) and TNFα (red) immunoreactivity in the margin incision skin. Colocalization (merge) is shown in yellow/orange. The left columns of both groups are shown in lower magnification. The right columns of both groups are shown in higher magnification. Scale bars: 50 μm (lower magnification) and 20 μm (higher magnification). In C57BL/6J mice, the co-expression of cytokeratin and TNFα began to increase at one day after incision ( P

    Journal: Molecular Pain

    Article Title: Persistent proliferation of keratinocytes and prolonged expression of pronociceptive inflammatory mediators might be associated with the postoperative pain in KK mice

    doi: 10.1177/1744806920927284

    Figure Lengend Snippet: Plantar incision induced the TNFα expression in keratinocytes in margin incision skin in both C57BL/6J and KK mice. Representative confocal images of cytokeratin (green) and TNFα (red) immunoreactivity in the margin incision skin. Colocalization (merge) is shown in yellow/orange. The left columns of both groups are shown in lower magnification. The right columns of both groups are shown in higher magnification. Scale bars: 50 μm (lower magnification) and 20 μm (higher magnification). In C57BL/6J mice, the co-expression of cytokeratin and TNFα began to increase at one day after incision ( P

    Article Snippet: AntibodiesThe sources of primary antibodies in this experiment were as follows: Cytokeratin (clone AE1/AE3) (ma-1316, 1:100, Thermo Fisher Scientific, Waltham, MA); IL-1β (ab2105, 1:100, Abcam Systems, Cambridge, UK), TNF-α(ab6671, 1:100, Abcam Systems, Cambridge, UK), Proliferating cell nuclear antigen (PCNA) (GB11010, servicebio, 1:200, Wuhan, China).

    Techniques: Expressing, Mouse Assay

    The plantar incision induced the IL1β expression in keratinocytes in margin incision skin in both C57BL/6J mice and KK mice. Representative confocal images of cytokeratin (green) and IL1β(red) immunoreactivity in the margin incision skin. Colocalization (merge) is shown in yellow/orange. The left columns of both groups are shown in lower magnification. The right columns of both groups are shown in higher magnification. Scale bars: 50 μm (lower magnification) and 20 μm (higher magnification). In C57BL/6J mice, the co-expression of cytokeratin and IL1β gradually increased and reached the peak at three days after incision ( P

    Journal: Molecular Pain

    Article Title: Persistent proliferation of keratinocytes and prolonged expression of pronociceptive inflammatory mediators might be associated with the postoperative pain in KK mice

    doi: 10.1177/1744806920927284

    Figure Lengend Snippet: The plantar incision induced the IL1β expression in keratinocytes in margin incision skin in both C57BL/6J mice and KK mice. Representative confocal images of cytokeratin (green) and IL1β(red) immunoreactivity in the margin incision skin. Colocalization (merge) is shown in yellow/orange. The left columns of both groups are shown in lower magnification. The right columns of both groups are shown in higher magnification. Scale bars: 50 μm (lower magnification) and 20 μm (higher magnification). In C57BL/6J mice, the co-expression of cytokeratin and IL1β gradually increased and reached the peak at three days after incision ( P

    Article Snippet: AntibodiesThe sources of primary antibodies in this experiment were as follows: Cytokeratin (clone AE1/AE3) (ma-1316, 1:100, Thermo Fisher Scientific, Waltham, MA); IL-1β (ab2105, 1:100, Abcam Systems, Cambridge, UK), TNF-α(ab6671, 1:100, Abcam Systems, Cambridge, UK), Proliferating cell nuclear antigen (PCNA) (GB11010, servicebio, 1:200, Wuhan, China).

    Techniques: Expressing, Mouse Assay

    The differential proliferation of keratinocytes in the margin skin of the incision in C57BL/6J and KK mice. Representative confocal images of cytokeratin (a keratinocyte marker, green) and PCNA (red) immunoreactivity in the margin incision skin. Colocalization (merge) is shown in yellow/orange. Scale bars: 50 μm. In C57BL/6J mice, the epidermal thickness increased at one day after incision ( P

    Journal: Molecular Pain

    Article Title: Persistent proliferation of keratinocytes and prolonged expression of pronociceptive inflammatory mediators might be associated with the postoperative pain in KK mice

    doi: 10.1177/1744806920927284

    Figure Lengend Snippet: The differential proliferation of keratinocytes in the margin skin of the incision in C57BL/6J and KK mice. Representative confocal images of cytokeratin (a keratinocyte marker, green) and PCNA (red) immunoreactivity in the margin incision skin. Colocalization (merge) is shown in yellow/orange. Scale bars: 50 μm. In C57BL/6J mice, the epidermal thickness increased at one day after incision ( P

    Article Snippet: AntibodiesThe sources of primary antibodies in this experiment were as follows: Cytokeratin (clone AE1/AE3) (ma-1316, 1:100, Thermo Fisher Scientific, Waltham, MA); IL-1β (ab2105, 1:100, Abcam Systems, Cambridge, UK), TNF-α(ab6671, 1:100, Abcam Systems, Cambridge, UK), Proliferating cell nuclear antigen (PCNA) (GB11010, servicebio, 1:200, Wuhan, China).

    Techniques: Mouse Assay, Marker

    Cytokeratin expression in ovine OSE. Immunohistochemistry for (A) intact OSE cells ( in vivo ) and (B) isolated OSE cells ( in vitro ), at 600× magnification.

    Journal: Saudi Journal of Biological Sciences

    Article Title: In vitro regulation of sheep ovarian surface epithelium (OSE) proliferation by local ovarian factors

    doi: 10.1016/j.sjbs.2012.03.003

    Figure Lengend Snippet: Cytokeratin expression in ovine OSE. Immunohistochemistry for (A) intact OSE cells ( in vivo ) and (B) isolated OSE cells ( in vitro ), at 600× magnification.

    Article Snippet: Cells were fixed in ice-cold 80% methanol, air-dried then washed in PBS, followed by incubation for 20 min with 10% normal horse serum and further 30 min with cytokeratin antibody (1:50 mouse monoclonal cytokeratin Clone MNF116 Dako Cytomation, Denmark A/S) at room temperature.

    Techniques: Expressing, Immunohistochemistry, In Vivo, Isolation, In Vitro

    Abnormal eyelid epithelial differentiation in Fgfr2 CKO and Smad4 CKO mice. ( A-C ) Immunostaining for keratin 14 (K14) in frontal sections from wild-type (A), Fgfr2 CKO (B) and Smad4 CKO (C) embryos at E15.0. In wild-type eyelids (A), K14 staining was weak in the palpebral conjunctiva and was not detected in the bulbar conjunctiva (arrows). In Fgfr2 CKO (B) and Smad4 CKO (C) eyelids, K14 staining was strong and uniform in the palpebral and bulbar conjunctiva (arrows). ( D-F ) Immunostaining for keratin 10 (K10) in frontal sections from wild type (D), Fgfr2 CKO (E) and Smad4 CKO (F) embryos at E17.5. In wild-type eyelids (D), K10 staining is observed only in the epidermis; it is negative in the conjunctiva (arrow). In Fgfr2 CKO (E), K10 is found in epidermis and a few cells of the palpebral conjunctiva, although the majority of conjunctival cells do not express K10 (arrow). In Smad4 CKO (F), K10 is detected in the epidermis and in the majority of conjunctival cells (arrow). ( G-I ) Immunostaining for keratin 4 (K4) in frontal sections from wild-type (G), Fgfr2 CKO (H) and Smad4 CKO (I) embryos at E17.5. In wild-type eyelids (G), expression of K4 is continuous in the conjunctiva (arrow). In Fgfr2 CKO (H), K4 is found in most conjunctival cells (arrow). In Smad4 CKO (I), K4 is only detected in a few conjunctival cells; most conjunctival cells do not express K4 (arrow). Scale bars: 100 μm.

    Journal: Development (Cambridge, England)

    Article Title: FGF-regulated BMP signaling is required for eyelid closure and to specify conjunctival epithelial cell fate

    doi: 10.1242/dev.034082

    Figure Lengend Snippet: Abnormal eyelid epithelial differentiation in Fgfr2 CKO and Smad4 CKO mice. ( A-C ) Immunostaining for keratin 14 (K14) in frontal sections from wild-type (A), Fgfr2 CKO (B) and Smad4 CKO (C) embryos at E15.0. In wild-type eyelids (A), K14 staining was weak in the palpebral conjunctiva and was not detected in the bulbar conjunctiva (arrows). In Fgfr2 CKO (B) and Smad4 CKO (C) eyelids, K14 staining was strong and uniform in the palpebral and bulbar conjunctiva (arrows). ( D-F ) Immunostaining for keratin 10 (K10) in frontal sections from wild type (D), Fgfr2 CKO (E) and Smad4 CKO (F) embryos at E17.5. In wild-type eyelids (D), K10 staining is observed only in the epidermis; it is negative in the conjunctiva (arrow). In Fgfr2 CKO (E), K10 is found in epidermis and a few cells of the palpebral conjunctiva, although the majority of conjunctival cells do not express K10 (arrow). In Smad4 CKO (F), K10 is detected in the epidermis and in the majority of conjunctival cells (arrow). ( G-I ) Immunostaining for keratin 4 (K4) in frontal sections from wild-type (G), Fgfr2 CKO (H) and Smad4 CKO (I) embryos at E17.5. In wild-type eyelids (G), expression of K4 is continuous in the conjunctiva (arrow). In Fgfr2 CKO (H), K4 is found in most conjunctival cells (arrow). In Smad4 CKO (I), K4 is only detected in a few conjunctival cells; most conjunctival cells do not express K4 (arrow). Scale bars: 100 μm.

    Article Snippet: The keratin 14 and keratin 10 antibody was from Covance Research Products (Denver, PA, USA).

    Techniques: Mouse Assay, Immunostaining, Staining, Expressing

    Summary of the role of BMP signaling and its interaction with the other signaling pathways known or proposed to function in mouse eyelid development. ( A ) In eyelids that are deficient in BMP signaling, the conjunctival epithelial cells express epidermis-specific keratin 10 and form hair follicles near that lid margin. ( B ) The network of known or proposed signaling pathways controlling eyelid development. Observations made in this study are indicated by blue arrows, previous findings by orange arrows and findings confirmed in this study by green arrows. Our model suggests that epidermal cell fate is the default pathway and that BMP signaling is required for prospective conjunctival epithelial cells to suppress the epidermal differentiation pathway and become conjunctival cells. BMP signaling is not required to initiate the migrat ion of periderm cells at the lid margins, but is required for the expansion of these cells across the corneal surface. During this process, BMP signaling is required for the expression of Foxc1 and Foxc2, and for the full activation (phosphorylation) of c-Jun. BMP-dependent formation of active R-Smad-Smad4 complexes is required for the translocation of p-c-Jun into the nuclei of periderm cells, where it has been reported to increase the expression of the epidermal growth factor receptor.

    Journal: Development (Cambridge, England)

    Article Title: FGF-regulated BMP signaling is required for eyelid closure and to specify conjunctival epithelial cell fate

    doi: 10.1242/dev.034082

    Figure Lengend Snippet: Summary of the role of BMP signaling and its interaction with the other signaling pathways known or proposed to function in mouse eyelid development. ( A ) In eyelids that are deficient in BMP signaling, the conjunctival epithelial cells express epidermis-specific keratin 10 and form hair follicles near that lid margin. ( B ) The network of known or proposed signaling pathways controlling eyelid development. Observations made in this study are indicated by blue arrows, previous findings by orange arrows and findings confirmed in this study by green arrows. Our model suggests that epidermal cell fate is the default pathway and that BMP signaling is required for prospective conjunctival epithelial cells to suppress the epidermal differentiation pathway and become conjunctival cells. BMP signaling is not required to initiate the migrat ion of periderm cells at the lid margins, but is required for the expansion of these cells across the corneal surface. During this process, BMP signaling is required for the expression of Foxc1 and Foxc2, and for the full activation (phosphorylation) of c-Jun. BMP-dependent formation of active R-Smad-Smad4 complexes is required for the translocation of p-c-Jun into the nuclei of periderm cells, where it has been reported to increase the expression of the epidermal growth factor receptor.

    Article Snippet: The keratin 14 and keratin 10 antibody was from Covance Research Products (Denver, PA, USA).

    Techniques: Expressing, Activation Assay, Translocation Assay

    TRAIL expressing mice develop less preneoplastic lesions compared to wild-type animals . (A) Representative images of the histologic structure of the lesions provoqued by the treatment. DMBA/TPA treated mice develop three different types of lesions: hamartomas, trichofolliculomas and papillomas, the two first being benign whereas the last one is a preneoplastic lesion. Bar, 400 μm. (B) Representative immunofluorescence images of hamartoma and papilloma lesions stained with Keratin 1 and α6 integrin. Bar, 100 μm. (C) Percentage of benign versus preneoplastic lesions in TRAIL transgenic mice compared to wild-type mice. The difference observed between wild-type and TRAIL transgenic lesions is highly significant (P

    Journal: Molecular Cancer

    Article Title: Targeted expression of tumor necrosis factor-related apoptosis-inducing ligand TRAIL in skin protects mice against chemical carcinogenesis

    doi: 10.1186/1476-4598-10-34

    Figure Lengend Snippet: TRAIL expressing mice develop less preneoplastic lesions compared to wild-type animals . (A) Representative images of the histologic structure of the lesions provoqued by the treatment. DMBA/TPA treated mice develop three different types of lesions: hamartomas, trichofolliculomas and papillomas, the two first being benign whereas the last one is a preneoplastic lesion. Bar, 400 μm. (B) Representative immunofluorescence images of hamartoma and papilloma lesions stained with Keratin 1 and α6 integrin. Bar, 100 μm. (C) Percentage of benign versus preneoplastic lesions in TRAIL transgenic mice compared to wild-type mice. The difference observed between wild-type and TRAIL transgenic lesions is highly significant (P

    Article Snippet: Keratin 1 antibody (Covance Dabco) was diluted at 1/500e and α6-integrin antibody (BD Pharmingen) was diluted at 1/200e .

    Techniques: Expressing, Mouse Assay, Immunofluorescence, Staining, Transgenic Assay

    SIG-1191 increases AQP3 gene expression and protein levels in Reconstructed Human Epidermis (RHE). EpiDerm-FT™ air–liquid interface cultures were topically treated with 0.25–0.5% (w/v) of SIG-1191 for 24 h. a AQP3 gene expression was analyzed by qPCR normalized to GAPDH a control housekeeping gene. The data represent the mean ± SEM of a representative experiment. b Haematoxylin and eosin (H E) staining and immunohistochemistry (IHC) of EpiDerm-FT™ tissues. Immunohistochemistry was performed with anti-aquaporin-3 ( green ), anti-keratin-10 ( red ), anti-rabbit Alexa-488, anti-mouseAlexa-594 antibodies. Sections were counterstained with 2-(4-amidinophenyl)-1H-indole-6-carboxamidine (DAPI). Merged micrograph shows overlaying K10 and AQP3 antibodies with DAPI staining. No background fluorescence was observed in the absence of the primary antibody (not shown). Original magnification: ×400

    Journal: Archives of Dermatological Research

    Article Title: N-Acetylglutaminoyl-S-farnesyl-l-cysteine (SIG-1191): an anti-inflammatory molecule that increases the expression of the aquaglyceroporin, aquaporin-3, in human keratinocytes

    doi: 10.1007/s00403-016-1708-x

    Figure Lengend Snippet: SIG-1191 increases AQP3 gene expression and protein levels in Reconstructed Human Epidermis (RHE). EpiDerm-FT™ air–liquid interface cultures were topically treated with 0.25–0.5% (w/v) of SIG-1191 for 24 h. a AQP3 gene expression was analyzed by qPCR normalized to GAPDH a control housekeeping gene. The data represent the mean ± SEM of a representative experiment. b Haematoxylin and eosin (H E) staining and immunohistochemistry (IHC) of EpiDerm-FT™ tissues. Immunohistochemistry was performed with anti-aquaporin-3 ( green ), anti-keratin-10 ( red ), anti-rabbit Alexa-488, anti-mouseAlexa-594 antibodies. Sections were counterstained with 2-(4-amidinophenyl)-1H-indole-6-carboxamidine (DAPI). Merged micrograph shows overlaying K10 and AQP3 antibodies with DAPI staining. No background fluorescence was observed in the absence of the primary antibody (not shown). Original magnification: ×400

    Article Snippet: Immunohistochemistry (IHC) was performed using anti-aquaporin-3 (AbCam; Cambridge, UK) and anti-keratin-10 (EMD Millipore; Billerica, MA).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Staining, Immunohistochemistry, Fluorescence

    Human periodontal ligament cells at passage three stained (A) positive for vimentin and (B) negative for cytokeratin. Blue indicates DAPI staining of nuclei (magnification ×200).

    Journal: Experimental and Therapeutic Medicine

    Article Title: Salidroside promotes human periodontal ligament cell proliferation and osteocalcin secretion via ERK1/2 and PI3K/Akt signaling pathways

    doi: 10.3892/etm.2018.6006

    Figure Lengend Snippet: Human periodontal ligament cells at passage three stained (A) positive for vimentin and (B) negative for cytokeratin. Blue indicates DAPI staining of nuclei (magnification ×200).

    Article Snippet: Next, hPDLCs were incubated with primary antibodies against vimentin (ab8978, 1/1000; Abcam Inc, Cambridge, MA, USA) and cytokeratin (1:200; cat. no. ab9377; Abcam, Cambridge, MA, USA) at 4°C for 12 h, and subsequently incubated with horseradish peroxidase (HRP)-conjugated secondary antibody (1:200; cat. no. ab191866; Abcam) for 1 h at room temperature.

    Techniques: Staining