cytokeratin 5 krt5 rabbit polyclonal antibody Search Results


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  • 93
    Thermo Fisher rabbit polyclonal krt5
    Rabbit Polyclonal Krt5, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Covance cytokeratin 5 krt5 rabbit polyclonal antibody
    Cytokeratin 5 Krt5 Rabbit Polyclonal Antibody, supplied by Covance, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    BioLegend rabbit anti krt5
    Mutant Kras expression in Dll1-positive cells leads to skin lesions at various sites. (A) Images of Dll1 CreERT2-ires-EGFP ; Kras LSL-G12D bi-transgenic mice, following tamoxifen injection. (B) Low (left) and high (right) magnification images after Hematoxylin/Eosin staining of skin lesions from mouth/lips of the above transgenic mice. (C) CLSM images of mouth/lips lesions, indicating cytokeratin expression patterns. (D) CLSM images indicating the existence of rare cells in the skin of mouth/lips areas of transgenic mice. GFP-expressing cells, indicating Dll1 CreERT2-ires-EGFP -dependent recombination, are located both within the basal <t>KRT5-positive</t> compartment, as well as in more superficial cells. In all fluorescence images DAPI was used as nuclear counterstain.
    Rabbit Anti Krt5, supplied by BioLegend, used in various techniques. Bioz Stars score: 96/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Covance keratin 5 k5 rabbit polyclonal
    Mutant Kras expression in Dll1-positive cells leads to skin lesions at various sites. (A) Images of Dll1 CreERT2-ires-EGFP ; Kras LSL-G12D bi-transgenic mice, following tamoxifen injection. (B) Low (left) and high (right) magnification images after Hematoxylin/Eosin staining of skin lesions from mouth/lips of the above transgenic mice. (C) CLSM images of mouth/lips lesions, indicating cytokeratin expression patterns. (D) CLSM images indicating the existence of rare cells in the skin of mouth/lips areas of transgenic mice. GFP-expressing cells, indicating Dll1 CreERT2-ires-EGFP -dependent recombination, are located both within the basal <t>KRT5-positive</t> compartment, as well as in more superficial cells. In all fluorescence images DAPI was used as nuclear counterstain.
    Keratin 5 K5 Rabbit Polyclonal, supplied by Covance, used in various techniques. Bioz Stars score: 85/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Covance goat anti rabbit cytokeratin 5 k5 polyclonal antibody prb 160b polyclonal antibody prb 160b igg
    Mutant Kras expression in Dll1-positive cells leads to skin lesions at various sites. (A) Images of Dll1 CreERT2-ires-EGFP ; Kras LSL-G12D bi-transgenic mice, following tamoxifen injection. (B) Low (left) and high (right) magnification images after Hematoxylin/Eosin staining of skin lesions from mouth/lips of the above transgenic mice. (C) CLSM images of mouth/lips lesions, indicating cytokeratin expression patterns. (D) CLSM images indicating the existence of rare cells in the skin of mouth/lips areas of transgenic mice. GFP-expressing cells, indicating Dll1 CreERT2-ires-EGFP -dependent recombination, are located both within the basal <t>KRT5-positive</t> compartment, as well as in more superficial cells. In all fluorescence images DAPI was used as nuclear counterstain.
    Goat Anti Rabbit Cytokeratin 5 K5 Polyclonal Antibody Prb 160b Polyclonal Antibody Prb 160b Igg, supplied by Covance, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Biorbyt keratin 5 antibody
    Mutant Kras expression in Dll1-positive cells leads to skin lesions at various sites. (A) Images of Dll1 CreERT2-ires-EGFP ; Kras LSL-G12D bi-transgenic mice, following tamoxifen injection. (B) Low (left) and high (right) magnification images after Hematoxylin/Eosin staining of skin lesions from mouth/lips of the above transgenic mice. (C) CLSM images of mouth/lips lesions, indicating cytokeratin expression patterns. (D) CLSM images indicating the existence of rare cells in the skin of mouth/lips areas of transgenic mice. GFP-expressing cells, indicating Dll1 CreERT2-ires-EGFP -dependent recombination, are located both within the basal <t>KRT5-positive</t> compartment, as well as in more superficial cells. In all fluorescence images DAPI was used as nuclear counterstain.
    Keratin 5 Antibody, supplied by Biorbyt, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Covance polyclonal rabbit anti mouse cytokeratin 5
    Mutant Kras expression in Dll1-positive cells leads to skin lesions at various sites. (A) Images of Dll1 CreERT2-ires-EGFP ; Kras LSL-G12D bi-transgenic mice, following tamoxifen injection. (B) Low (left) and high (right) magnification images after Hematoxylin/Eosin staining of skin lesions from mouth/lips of the above transgenic mice. (C) CLSM images of mouth/lips lesions, indicating cytokeratin expression patterns. (D) CLSM images indicating the existence of rare cells in the skin of mouth/lips areas of transgenic mice. GFP-expressing cells, indicating Dll1 CreERT2-ires-EGFP -dependent recombination, are located both within the basal <t>KRT5-positive</t> compartment, as well as in more superficial cells. In all fluorescence images DAPI was used as nuclear counterstain.
    Polyclonal Rabbit Anti Mouse Cytokeratin 5, supplied by Covance, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Mutant Kras expression in Dll1-positive cells leads to skin lesions at various sites. (A) Images of Dll1 CreERT2-ires-EGFP ; Kras LSL-G12D bi-transgenic mice, following tamoxifen injection. (B) Low (left) and high (right) magnification images after Hematoxylin/Eosin staining of skin lesions from mouth/lips of the above transgenic mice. (C) CLSM images of mouth/lips lesions, indicating cytokeratin expression patterns. (D) CLSM images indicating the existence of rare cells in the skin of mouth/lips areas of transgenic mice. GFP-expressing cells, indicating Dll1 CreERT2-ires-EGFP -dependent recombination, are located both within the basal KRT5-positive compartment, as well as in more superficial cells. In all fluorescence images DAPI was used as nuclear counterstain.

    Journal: Translational Oncology

    Article Title: Dll1 Marks Cells of Origin of Ras-Induced Cancer in Mouse Squamous Epithelia

    doi: 10.1016/j.tranon.2018.07.011

    Figure Lengend Snippet: Mutant Kras expression in Dll1-positive cells leads to skin lesions at various sites. (A) Images of Dll1 CreERT2-ires-EGFP ; Kras LSL-G12D bi-transgenic mice, following tamoxifen injection. (B) Low (left) and high (right) magnification images after Hematoxylin/Eosin staining of skin lesions from mouth/lips of the above transgenic mice. (C) CLSM images of mouth/lips lesions, indicating cytokeratin expression patterns. (D) CLSM images indicating the existence of rare cells in the skin of mouth/lips areas of transgenic mice. GFP-expressing cells, indicating Dll1 CreERT2-ires-EGFP -dependent recombination, are located both within the basal KRT5-positive compartment, as well as in more superficial cells. In all fluorescence images DAPI was used as nuclear counterstain.

    Article Snippet: Catalogue numbers and dilutions of the antibodies herein used are given as following: chicken anti-Krt14 (Biolegend #906001, 1:250), rabbit anti-Krt5 (Biolegend #905501, 1:1000), guinea pig anti-Krt10 (Progen Biotechnik #GP-K10, 1:200), rat anti-Troma I (DSHB #AB531826, 1:100), rabbit anti-Calbindin (Swant #CB38a, 1:500), sheep anti-Dll1 (R & D Systems #AF5026) and rabbit anti-E-cadherin (Cell Signaling Technology #3195S, 1:200).

    Techniques: Mutagenesis, Expressing, Transgenic Assay, Mouse Assay, Injection, Staining, Confocal Laser Scanning Microscopy, Fluorescence

    IBV survivor cell populations are predominantly composed of cells expressing ciliated cell markers. a Tracheas were collected from Mock and Mal/04-Cre infected mice at 2 and 14 DPI then opened longitudinally and flattened to image the surface area of the whole-mount tracheal epithelium. Scale bar = 25 μm. b Quantification of apical surface area covered by tdTomato+ survivor cells from whole-mount trachea images. n = 4, 12 images per group, respectively, Student’s T -test. c Flow cytometry of 14 DPI tdTomato- (uninfected cells) and 14 DPI tdTomato+ (survivor cells) from the lungs of Mal/04-Cre infected mice, where cells are stained with CD24 (ciliated), SSEA-1 (secretory), GSIB4 (basal) cell surface markers. d Quantification of flow cytometry. n = 3 mice per group. e Cross-sectioned microscopy of murine tracheal epithelial cells from mice 14 days post-infection with Mal/04-Cre stained with KRT5 to label basal stem cells and SSEA-1 to label secretory cells. f Cross-sectioned microscopy of murine tracheal epithelial cells from mice 14 days post-infection with Mal/04-Cre stained with FOXJ1 to label ciliated cells. g Quantification of tdTomato+ survivor cells stained with cell-specific markers: KRT5, SSEA-1, and FOXJ1 compiled from two independent experiments. Data are presented as the number of marker+ cells out of total tdTomato+ cells and the percentage was calculated for marker+ cells out of total tdTomato+ cells imaged. S.E.M was plotted to indicate variability around the mean for tested samples, * p ≤ 0.05, ** p ≤ 0.001, ns not significant

    Journal: Nature Communications

    Article Title: Non-lytic clearance of influenza B virus from infected cells preserves epithelial barrier function

    doi: 10.1038/s41467-019-08617-z

    Figure Lengend Snippet: IBV survivor cell populations are predominantly composed of cells expressing ciliated cell markers. a Tracheas were collected from Mock and Mal/04-Cre infected mice at 2 and 14 DPI then opened longitudinally and flattened to image the surface area of the whole-mount tracheal epithelium. Scale bar = 25 μm. b Quantification of apical surface area covered by tdTomato+ survivor cells from whole-mount trachea images. n = 4, 12 images per group, respectively, Student’s T -test. c Flow cytometry of 14 DPI tdTomato- (uninfected cells) and 14 DPI tdTomato+ (survivor cells) from the lungs of Mal/04-Cre infected mice, where cells are stained with CD24 (ciliated), SSEA-1 (secretory), GSIB4 (basal) cell surface markers. d Quantification of flow cytometry. n = 3 mice per group. e Cross-sectioned microscopy of murine tracheal epithelial cells from mice 14 days post-infection with Mal/04-Cre stained with KRT5 to label basal stem cells and SSEA-1 to label secretory cells. f Cross-sectioned microscopy of murine tracheal epithelial cells from mice 14 days post-infection with Mal/04-Cre stained with FOXJ1 to label ciliated cells. g Quantification of tdTomato+ survivor cells stained with cell-specific markers: KRT5, SSEA-1, and FOXJ1 compiled from two independent experiments. Data are presented as the number of marker+ cells out of total tdTomato+ cells and the percentage was calculated for marker+ cells out of total tdTomato+ cells imaged. S.E.M was plotted to indicate variability around the mean for tested samples, * p ≤ 0.05, ** p ≤ 0.001, ns not significant

    Article Snippet: Samples were then stained with Actub (Abcam, clone EPR16772, cat ab179484, 1:1000), Krt5 (Biolegend, clone Poly19055, cat 905501, 1:500), FoxJ1 (eBioscience, clone 2A5, cat 14-9965, 1:500), SSEA-1 (Biolegend, clone MC-480, cat 125611, 1:250) ZO-1 (ThermoFisher, clone ZO-1-1A12, cat 33-9100, 1:500), CD24 (Abcam, clone M1/69, cat ab64064, 1:250) (Supplementary Table ).

    Techniques: Expressing, Infection, Mouse Assay, Flow Cytometry, Cytometry, Staining, Microscopy, Marker

    Loss of Jag ligands results in expansion of the embryonic basal cells and multiciliated cells in the E18.5 extrapulmonary airways (trachea). IF of basal cells ( A : Krt5, B: p63) and multiciliated cells ( A : beta-tubulin 4; B: Foxj1) in control and double Jag1 cnull ; Jag2 cnull tracheas. ( C ) Morphometric analysis of basal cells (top: p63+) and multiciliated cells (bottom: Foxj1 +) in single and double Jag mutant tracheas. Sections of whole trachea were counted for p63+ and DAPI+ cells and the percentage of p63+ cells was determined in airway epithelium. Graphs are mean ± SEM; **p

    Journal: eLife

    Article Title: Jagged and Delta-like ligands control distinct events during airway progenitor cell differentiation

    doi: 10.7554/eLife.50487

    Figure Lengend Snippet: Loss of Jag ligands results in expansion of the embryonic basal cells and multiciliated cells in the E18.5 extrapulmonary airways (trachea). IF of basal cells ( A : Krt5, B: p63) and multiciliated cells ( A : beta-tubulin 4; B: Foxj1) in control and double Jag1 cnull ; Jag2 cnull tracheas. ( C ) Morphometric analysis of basal cells (top: p63+) and multiciliated cells (bottom: Foxj1 +) in single and double Jag mutant tracheas. Sections of whole trachea were counted for p63+ and DAPI+ cells and the percentage of p63+ cells was determined in airway epithelium. Graphs are mean ± SEM; **p

    Article Snippet: Antibodies used were: anti-β-tubulin IV (Abcam #ab11315, 1:100), anti-Ascl1 (Thermo Fisher #14-5794-82, 1:100), anti-CC10 (Santa Cruz sc9772, 1:150), anti-Cgrp (Sigma Aldrich #C8198, 1:2500), anti-Foxj1 (Thermo Fisher #14-9965-80, 1:50), anti-Ki67 (Cell Signaling #9129, 1:100), anti-Krt5 (Biolegend #905501, 1:500), anti-Krt8 (Abcam #ab107115, 1:500); anti-N1ICD (Cell Signaling #4147, 1:100), anti-p63 (Santa Cruz #sc8343, 1:400), anti-Scgb3a2 (R and D Systems #AF3465, 1:100), anti-SSEA1 (EMD Millipore #MAB4301, 1:300).

    Techniques: Mutagenesis