cyp2d6 gene Search Results


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  • 93
    Thermo Fisher gene exp cyp2d6 hs02576167 m1
    Identification of genetic variants of <t>CYP2D6</t> with mutations in putative targeting signals. (A and B) Total RNA was extracted from human liver samples and the first 600 nucleotides of the coding region were amplified by RT-PCR. The amplified DNA was cloned and sequenced. (A) Schematic representations of point mutations identified in the human liver samples. (B) Schematic representations of splice variants identified. The deleted or skipped exon regions are shown as hatched boxes.
    Gene Exp Cyp2d6 Hs02576167 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher gene exp cyp2d6 hs03043790 g1
    Effect of β-NF and EtOH treatment on cytochrome P450 (CYP) isoforms’ expression in undifferentiated SH-SY5Y cells. ( a – d ) The graphs show the relative protein levels quantification by Western blot for the <t>CYP2D6</t> ( a ), 2E1 ( b ), 1A1 ( c ), and 3A4 ( d ). Data was acquired by measuring the fluorescent intensity per pixel on each band. The relative amount of protein normalised with β-actin as a housekeeping protein for each condition was plotted as fold-increase and compared to the control, which was given a value of 1. Columns represent the mean ± SEM of at least three different experiments. Statistical significance was analyzed by one-way ANOVA followed by a Tukey post-test (* p
    Gene Exp Cyp2d6 Hs03043790 G1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Xenobiotics cyp2d6 gene
    Effect of β-NF and EtOH treatment on cytochrome P450 (CYP) isoforms’ expression in undifferentiated SH-SY5Y cells. ( a – d ) The graphs show the relative protein levels quantification by Western blot for the <t>CYP2D6</t> ( a ), 2E1 ( b ), 1A1 ( c ), and 3A4 ( d ). Data was acquired by measuring the fluorescent intensity per pixel on each band. The relative amount of protein normalised with β-actin as a housekeeping protein for each condition was plotted as fold-increase and compared to the control, which was given a value of 1. Columns represent the mean ± SEM of at least three different experiments. Statistical significance was analyzed by one-way ANOVA followed by a Tukey post-test (* p
    Cyp2d6 Gene, supplied by Xenobiotics, used in various techniques. Bioz Stars score: 88/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Luminex cyp2d6 gene
    Effect of β-NF and EtOH treatment on cytochrome P450 (CYP) isoforms’ expression in undifferentiated SH-SY5Y cells. ( a – d ) The graphs show the relative protein levels quantification by Western blot for the <t>CYP2D6</t> ( a ), 2E1 ( b ), 1A1 ( c ), and 3A4 ( d ). Data was acquired by measuring the fluorescent intensity per pixel on each band. The relative amount of protein normalised with β-actin as a housekeeping protein for each condition was plotted as fold-increase and compared to the control, which was given a value of 1. Columns represent the mean ± SEM of at least three different experiments. Statistical significance was analyzed by one-way ANOVA followed by a Tukey post-test (* p
    Cyp2d6 Gene, supplied by Luminex, used in various techniques. Bioz Stars score: 88/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher cyp2d6 genes
    CpG island of CYP2E1 and <t>CYP2D6</t> gene. Note: ( A ) CYP2E1 gene CpG island; ( B ) CYP2D6 gene CpG island.
    Cyp2d6 Genes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Genewiz cyp2d6 10 genes
    CpG island of CYP2E1 and <t>CYP2D6</t> gene. Note: ( A ) CYP2E1 gene CpG island; ( B ) CYP2D6 gene CpG island.
    Cyp2d6 10 Genes, supplied by Genewiz, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher cyp2d6 gene boundary
    CpG island of CYP2E1 and <t>CYP2D6</t> gene. Note: ( A ) CYP2E1 gene CpG island; ( B ) CYP2D6 gene CpG island.
    Cyp2d6 Gene Boundary, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    4Gene cyp2d6 4 gene polymorphism
    CpG island of CYP2E1 and <t>CYP2D6</t> gene. Note: ( A ) CYP2E1 gene CpG island; ( B ) CYP2D6 gene CpG island.
    Cyp2d6 4 Gene Polymorphism, supplied by 4Gene, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    4Gene duplicated cyp2d6 4 gene
    CYP2D6*17 (1023C > T) TaqMan Assay Genotype Results. (Panels A and B) show the scatter plots for TaqMan assays C__2222771_40 (original assay) and C__2222771_A0 (alternative assay). Numbered subjects correspond to cases 1–9 and 21–23 ( Tables 1 and 2 ). Cases 1–9 were identified as 1023 T/T with the original assay (panel A), but genotyped accurately as 1023 C/T with the alternative assay (panel B). Additional samples of known genotypes were analyzed as references to allow cluster formation in the scatter plots; none of these controls carry a <t>CYP2D6*4</t> .
    Duplicated Cyp2d6 4 Gene, supplied by 4Gene, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    4Gene cyp2d6 4 gene duplication
    CYP2D6*17 (1023C > T) TaqMan Assay Genotype Results. (Panels A and B) show the scatter plots for TaqMan assays C__2222771_40 (original assay) and C__2222771_A0 (alternative assay). Numbered subjects correspond to cases 1–9 and 21–23 ( Tables 1 and 2 ). Cases 1–9 were identified as 1023 T/T with the original assay (panel A), but genotyped accurately as 1023 C/T with the alternative assay (panel B). Additional samples of known genotypes were analyzed as references to allow cluster formation in the scatter plots; none of these controls carry a <t>CYP2D6*4</t> .
    Cyp2d6 4 Gene Duplication, supplied by 4Gene, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher copy number variation cyp2d6 hs00010001 cn
    PCR fragment analysis. The PCR products for amplicons F–J were used in fragment analysis on the Agilent Technologies DNA 7500 Kit to show the presence or absence of the CYP2D7 gene, <t>CYP2D6</t> genes, or recombinant allele. Fragments A–E are not shown as these were primarily useful for the generation of amplicons used in allele-specific primer extension assay reactions and sequencing reactions. Genotypes tested are shown at the top of each lane. The ladder in the left column shows the size in base pairs of the molecular weight marker. The uppermost purple band in all sample lanes represents a marker depicting the maximum size that the kit can detect reliably. (a) PCR fragment F analysis detects CYP2D7 exon 9 through the region 3′ of rep 6. The * 68 +* 4-like and * 4N +* 4-like arrangements did not produce a product because the * 4-like allele did not have the exon 9 conversion present. The sample containing the * 5 /* 5 genotype and those with single * 36 , * 61 , and * 63 alleles generated an amplicon. The size of the product is consistent with the presence of the CYP2D7 -derived spacer sequence which was confirmed by DNA sequencing. (b) PCR fragment G analysis that detects CYP2D7/CYP2D6 genes. Only samples with * 13 and * 16 generated an amplicon. (c) PCR fragment H analysis detects DNA spanning from the CYP2D7 promoter to CYP2D6 promoter regions. The presence or absence of 1.6 kb spacer sequence can be visualized based on the amplicon size. Genotype * 5 /* 5 lacks the CYP2D6 promoter region, so does not generate an amplicon. Genotype * 13 +* 2A /* 5 lacks the 1.6 kb spacer sequence, so the amplicon is smaller than the products generated by the other genotypes tested. (d) PCR fragment I analysis generates an amplicon spanning from CYP2D8 3′region to a unique region in CYP2D6 intron 3. The * 13 +* 2A -containing sample generated an amplicon because CYP2D7 was absent. No amplicon was detected in * 16 samples because the crossover region in * 16 occurs after exon 7, which eliminates the reverse primer binding site for the amplicons. Normal gene arrangements and typical duplication arrangements do not generate a product because the size of the amplicons is too great. (e) PCR fragment J analysis generates amplicons spanning from the CYP2D8 3′ region to the CYP2D6 -specific trailing sequences. Amplicons are generated for the * 13 +* 2A -containing and * 16 -containing samples because CYP2D7 is absent in these samples. Normal gene arrangements and typical duplication arrangements do not generate a product because the size of the amplicons is too great.
    Copy Number Variation Cyp2d6 Hs00010001 Cn, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 97 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    98
    Thermo Fisher gene exp loc101929829 hs00164385 m1
    PCR fragment analysis. The PCR products for amplicons F–J were used in fragment analysis on the Agilent Technologies DNA 7500 Kit to show the presence or absence of the CYP2D7 gene, <t>CYP2D6</t> genes, or recombinant allele. Fragments A–E are not shown as these were primarily useful for the generation of amplicons used in allele-specific primer extension assay reactions and sequencing reactions. Genotypes tested are shown at the top of each lane. The ladder in the left column shows the size in base pairs of the molecular weight marker. The uppermost purple band in all sample lanes represents a marker depicting the maximum size that the kit can detect reliably. (a) PCR fragment F analysis detects CYP2D7 exon 9 through the region 3′ of rep 6. The * 68 +* 4-like and * 4N +* 4-like arrangements did not produce a product because the * 4-like allele did not have the exon 9 conversion present. The sample containing the * 5 /* 5 genotype and those with single * 36 , * 61 , and * 63 alleles generated an amplicon. The size of the product is consistent with the presence of the CYP2D7 -derived spacer sequence which was confirmed by DNA sequencing. (b) PCR fragment G analysis that detects CYP2D7/CYP2D6 genes. Only samples with * 13 and * 16 generated an amplicon. (c) PCR fragment H analysis detects DNA spanning from the CYP2D7 promoter to CYP2D6 promoter regions. The presence or absence of 1.6 kb spacer sequence can be visualized based on the amplicon size. Genotype * 5 /* 5 lacks the CYP2D6 promoter region, so does not generate an amplicon. Genotype * 13 +* 2A /* 5 lacks the 1.6 kb spacer sequence, so the amplicon is smaller than the products generated by the other genotypes tested. (d) PCR fragment I analysis generates an amplicon spanning from CYP2D8 3′region to a unique region in CYP2D6 intron 3. The * 13 +* 2A -containing sample generated an amplicon because CYP2D7 was absent. No amplicon was detected in * 16 samples because the crossover region in * 16 occurs after exon 7, which eliminates the reverse primer binding site for the amplicons. Normal gene arrangements and typical duplication arrangements do not generate a product because the size of the amplicons is too great. (e) PCR fragment J analysis generates amplicons spanning from the CYP2D8 3′ region to the CYP2D6 -specific trailing sequences. Amplicons are generated for the * 13 +* 2A -containing and * 16 -containing samples because CYP2D7 is absent in these samples. Normal gene arrangements and typical duplication arrangements do not generate a product because the size of the amplicons is too great.
    Gene Exp Loc101929829 Hs00164385 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    fluidigm cyp2d6 allele frequencies
    The cytochrome P450 ( CYP ) 2D6 activity scores associated with tamoxifen metabolites and metabolic ratios. Activity scores for <t>CYP2D6</t> were associated with steady‐state plasma concentrations of ( a ) endoxifen and ( b ) 4‐hydroxytamoxifen (4‐OH‐Tam). The CYP2D6 activity scores were also associated with metabolic ratios of ( c ) endoxifen/tamoxifen, ( d ) 4‐OH‐Tam/tamoxifen, ( e ) endoxifen/4‐OH‐Tam, and ( f ) endoxifen/N‐desmethyltamoxifen ( N ‐dm‐Tam). Genotype‐phenotype associations with P
    Cyp2d6 Allele Frequencies, supplied by fluidigm, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Luminex tag it mutation detection kit p450 cyp2d6 version 1
    The cytochrome P450 ( CYP ) 2D6 activity scores associated with tamoxifen metabolites and metabolic ratios. Activity scores for <t>CYP2D6</t> were associated with steady‐state plasma concentrations of ( a ) endoxifen and ( b ) 4‐hydroxytamoxifen (4‐OH‐Tam). The CYP2D6 activity scores were also associated with metabolic ratios of ( c ) endoxifen/tamoxifen, ( d ) 4‐OH‐Tam/tamoxifen, ( e ) endoxifen/4‐OH‐Tam, and ( f ) endoxifen/N‐desmethyltamoxifen ( N ‐dm‐Tam). Genotype‐phenotype associations with P
    Tag It Mutation Detection Kit P450 Cyp2d6 Version 1, supplied by Luminex, used in various techniques. Bioz Stars score: 85/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher cyp2d6 taqman snp genotyping assays
    The cytochrome P450 ( CYP ) 2D6 activity scores associated with tamoxifen metabolites and metabolic ratios. Activity scores for <t>CYP2D6</t> were associated with steady‐state plasma concentrations of ( a ) endoxifen and ( b ) 4‐hydroxytamoxifen (4‐OH‐Tam). The CYP2D6 activity scores were also associated with metabolic ratios of ( c ) endoxifen/tamoxifen, ( d ) 4‐OH‐Tam/tamoxifen, ( e ) endoxifen/4‐OH‐Tam, and ( f ) endoxifen/N‐desmethyltamoxifen ( N ‐dm‐Tam). Genotype‐phenotype associations with P
    Cyp2d6 Taqman Snp Genotyping Assays, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher dmet plus microarray
    The cytochrome P450 ( CYP ) 2D6 activity scores associated with tamoxifen metabolites and metabolic ratios. Activity scores for <t>CYP2D6</t> were associated with steady‐state plasma concentrations of ( a ) endoxifen and ( b ) 4‐hydroxytamoxifen (4‐OH‐Tam). The CYP2D6 activity scores were also associated with metabolic ratios of ( c ) endoxifen/tamoxifen, ( d ) 4‐OH‐Tam/tamoxifen, ( e ) endoxifen/4‐OH‐Tam, and ( f ) endoxifen/N‐desmethyltamoxifen ( N ‐dm‐Tam). Genotype‐phenotype associations with P
    Dmet Plus Microarray, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher snp ndufa6 as1 c 32407245 60
    The cytochrome P450 ( CYP ) 2D6 activity scores associated with tamoxifen metabolites and metabolic ratios. Activity scores for <t>CYP2D6</t> were associated with steady‐state plasma concentrations of ( a ) endoxifen and ( b ) 4‐hydroxytamoxifen (4‐OH‐Tam). The CYP2D6 activity scores were also associated with metabolic ratios of ( c ) endoxifen/tamoxifen, ( d ) 4‐OH‐Tam/tamoxifen, ( e ) endoxifen/4‐OH‐Tam, and ( f ) endoxifen/N‐desmethyltamoxifen ( N ‐dm‐Tam). Genotype‐phenotype associations with P
    Snp Ndufa6 As1 C 32407245 60, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Xenobiotics cytochrome p450
    The cytochrome P450 ( CYP ) 2D6 activity scores associated with tamoxifen metabolites and metabolic ratios. Activity scores for <t>CYP2D6</t> were associated with steady‐state plasma concentrations of ( a ) endoxifen and ( b ) 4‐hydroxytamoxifen (4‐OH‐Tam). The CYP2D6 activity scores were also associated with metabolic ratios of ( c ) endoxifen/tamoxifen, ( d ) 4‐OH‐Tam/tamoxifen, ( e ) endoxifen/4‐OH‐Tam, and ( f ) endoxifen/N‐desmethyltamoxifen ( N ‐dm‐Tam). Genotype‐phenotype associations with P
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    Thermo Fisher 7500 real time pcr system
    The cytochrome P450 ( CYP ) 2D6 activity scores associated with tamoxifen metabolites and metabolic ratios. Activity scores for <t>CYP2D6</t> were associated with steady‐state plasma concentrations of ( a ) endoxifen and ( b ) 4‐hydroxytamoxifen (4‐OH‐Tam). The CYP2D6 activity scores were also associated with metabolic ratios of ( c ) endoxifen/tamoxifen, ( d ) 4‐OH‐Tam/tamoxifen, ( e ) endoxifen/4‐OH‐Tam, and ( f ) endoxifen/N‐desmethyltamoxifen ( N ‐dm‐Tam). Genotype‐phenotype associations with P
    7500 Real Time Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 54115 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Xenobiotics variable dna methylation
    Heat maps of the <t>DNA</t> methylation profiles of the representative 6 CYP and 2 control genes by cluster analysis. The top of the map indicates samples from 20 normal adult livers (L1 to L81, NLA and NL2), 1 fetal liver (NLF), 1 adult small intestine (NSI), and 3 hepatoma cell lines. The right of the map indicates the target ID and gene name ( <t>CYP1A2</t> , CYP1B1 , CYP2C9 , CYP2C19 , CYP2D6 , CYP3A4 , ACTB , and BMP4 ) examined for individual CpG sites. The representative methylation profiles of individual genes were selected by excluding profiles showing the similar patterns each other. Therefore, the hierarchical similarity tree was not shown in the map. Higher DNA methylation levels are shown in red , while lower DNA methylation levels are shown in black
    Variable Dna Methylation, supplied by Xenobiotics, used in various techniques. Bioz Stars score: 88/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    SinaClon BioScience genomic dna purification kit
    Heat maps of the <t>DNA</t> methylation profiles of the representative 6 CYP and 2 control genes by cluster analysis. The top of the map indicates samples from 20 normal adult livers (L1 to L81, NLA and NL2), 1 fetal liver (NLF), 1 adult small intestine (NSI), and 3 hepatoma cell lines. The right of the map indicates the target ID and gene name ( <t>CYP1A2</t> , CYP1B1 , CYP2C9 , CYP2C19 , CYP2D6 , CYP3A4 , ACTB , and BMP4 ) examined for individual CpG sites. The representative methylation profiles of individual genes were selected by excluding profiles showing the similar patterns each other. Therefore, the hierarchical similarity tree was not shown in the map. Higher DNA methylation levels are shown in red , while lower DNA methylation levels are shown in black
    Genomic Dna Purification Kit, supplied by SinaClon BioScience, used in various techniques. Bioz Stars score: 92/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PharmGenomics dna chip
    Heat maps of the <t>DNA</t> methylation profiles of the representative 6 CYP and 2 control genes by cluster analysis. The top of the map indicates samples from 20 normal adult livers (L1 to L81, NLA and NL2), 1 fetal liver (NLF), 1 adult small intestine (NSI), and 3 hepatoma cell lines. The right of the map indicates the target ID and gene name ( <t>CYP1A2</t> , CYP1B1 , CYP2C9 , CYP2C19 , CYP2D6 , CYP3A4 , ACTB , and BMP4 ) examined for individual CpG sites. The representative methylation profiles of individual genes were selected by excluding profiles showing the similar patterns each other. Therefore, the hierarchical similarity tree was not shown in the map. Higher DNA methylation levels are shown in red , while lower DNA methylation levels are shown in black
    Dna Chip, supplied by PharmGenomics, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher taqman drug metabolism genotyping assay mix
    Heat maps of the <t>DNA</t> methylation profiles of the representative 6 CYP and 2 control genes by cluster analysis. The top of the map indicates samples from 20 normal adult livers (L1 to L81, NLA and NL2), 1 fetal liver (NLF), 1 adult small intestine (NSI), and 3 hepatoma cell lines. The right of the map indicates the target ID and gene name ( <t>CYP1A2</t> , CYP1B1 , CYP2C9 , CYP2C19 , CYP2D6 , CYP3A4 , ACTB , and BMP4 ) examined for individual CpG sites. The representative methylation profiles of individual genes were selected by excluding profiles showing the similar patterns each other. Therefore, the hierarchical similarity tree was not shown in the map. Higher DNA methylation levels are shown in red , while lower DNA methylation levels are shown in black
    Taqman Drug Metabolism Genotyping Assay Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher snp cyp2d7 c 32407252 30
    Heat maps of the <t>DNA</t> methylation profiles of the representative 6 CYP and 2 control genes by cluster analysis. The top of the map indicates samples from 20 normal adult livers (L1 to L81, NLA and NL2), 1 fetal liver (NLF), 1 adult small intestine (NSI), and 3 hepatoma cell lines. The right of the map indicates the target ID and gene name ( <t>CYP1A2</t> , CYP1B1 , CYP2C9 , CYP2C19 , CYP2D6 , CYP3A4 , ACTB , and BMP4 ) examined for individual CpG sites. The representative methylation profiles of individual genes were selected by excluding profiles showing the similar patterns each other. Therefore, the hierarchical similarity tree was not shown in the map. Higher DNA methylation levels are shown in red , while lower DNA methylation levels are shown in black
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    Image Search Results


    Identification of genetic variants of CYP2D6 with mutations in putative targeting signals. (A and B) Total RNA was extracted from human liver samples and the first 600 nucleotides of the coding region were amplified by RT-PCR. The amplified DNA was cloned and sequenced. (A) Schematic representations of point mutations identified in the human liver samples. (B) Schematic representations of splice variants identified. The deleted or skipped exon regions are shown as hatched boxes.

    Journal: Molecular genetics and metabolism

    Article Title: Identification of Genetic Variants of Human Cytochrome P450 2D6 with Impaired Mitochondrial Targeting

    doi: 10.1016/j.ymgme.2009.08.009

    Figure Lengend Snippet: Identification of genetic variants of CYP2D6 with mutations in putative targeting signals. (A and B) Total RNA was extracted from human liver samples and the first 600 nucleotides of the coding region were amplified by RT-PCR. The amplified DNA was cloned and sequenced. (A) Schematic representations of point mutations identified in the human liver samples. (B) Schematic representations of splice variants identified. The deleted or skipped exon regions are shown as hatched boxes.

    Article Snippet: A TaqMan gene expression assay that was designed to detect just the exon 3-skipped splice variant form (Hs02576167_m1, Applied Biosystems) was used.

    Techniques: Amplification, Reverse Transcription Polymerase Chain Reaction, Clone Assay

    Effect of β-NF and EtOH treatment on cytochrome P450 (CYP) isoforms’ expression in undifferentiated SH-SY5Y cells. ( a – d ) The graphs show the relative protein levels quantification by Western blot for the CYP2D6 ( a ), 2E1 ( b ), 1A1 ( c ), and 3A4 ( d ). Data was acquired by measuring the fluorescent intensity per pixel on each band. The relative amount of protein normalised with β-actin as a housekeeping protein for each condition was plotted as fold-increase and compared to the control, which was given a value of 1. Columns represent the mean ± SEM of at least three different experiments. Statistical significance was analyzed by one-way ANOVA followed by a Tukey post-test (* p

    Journal: International Journal of Molecular Sciences

    Article Title: β-Naphtoflavone and Ethanol Induce Cytochrome P450 and Protect towards MPP+ Toxicity in Human Neuroblastoma SH-SY5Y Cells

    doi: 10.3390/ijms19113369

    Figure Lengend Snippet: Effect of β-NF and EtOH treatment on cytochrome P450 (CYP) isoforms’ expression in undifferentiated SH-SY5Y cells. ( a – d ) The graphs show the relative protein levels quantification by Western blot for the CYP2D6 ( a ), 2E1 ( b ), 1A1 ( c ), and 3A4 ( d ). Data was acquired by measuring the fluorescent intensity per pixel on each band. The relative amount of protein normalised with β-actin as a housekeeping protein for each condition was plotted as fold-increase and compared to the control, which was given a value of 1. Columns represent the mean ± SEM of at least three different experiments. Statistical significance was analyzed by one-way ANOVA followed by a Tukey post-test (* p

    Article Snippet: The following probes for CYP isoforms were used, CYP1A1 (Life Technologies, Assay ID Hs01054797_g1, # 4331182), CYP2D6 (Life Technologies, Assay ID Hs03043790_g1, # 4331182), and CYP2E1 (Life Technologies, Assay ID Hs00559368_m1, # 4453320).

    Techniques: Expressing, Western Blot

    mRNA levels of CYP2D6, 2E1, and 1A1 in SH-SY5Y cells treated with β-naphtoflavone (β-NF) and EtOH. The relative mRNA levels were measured by qRT-PCR for CYP 2D6 ( a ), CYP 2E1 ( b ), and CYP 1A1 ( c ). The results represent the mean ± SEM of at least three different experiments. Each column represents the fold increase calculated after a ΔΔ C t analysis of each treatment compared with controls and normalised with rRNA 18S as a housekeeping gene. Controls of each isoform were always taken as 1-fold increase. * p

    Journal: International Journal of Molecular Sciences

    Article Title: β-Naphtoflavone and Ethanol Induce Cytochrome P450 and Protect towards MPP+ Toxicity in Human Neuroblastoma SH-SY5Y Cells

    doi: 10.3390/ijms19113369

    Figure Lengend Snippet: mRNA levels of CYP2D6, 2E1, and 1A1 in SH-SY5Y cells treated with β-naphtoflavone (β-NF) and EtOH. The relative mRNA levels were measured by qRT-PCR for CYP 2D6 ( a ), CYP 2E1 ( b ), and CYP 1A1 ( c ). The results represent the mean ± SEM of at least three different experiments. Each column represents the fold increase calculated after a ΔΔ C t analysis of each treatment compared with controls and normalised with rRNA 18S as a housekeeping gene. Controls of each isoform were always taken as 1-fold increase. * p

    Article Snippet: The following probes for CYP isoforms were used, CYP1A1 (Life Technologies, Assay ID Hs01054797_g1, # 4331182), CYP2D6 (Life Technologies, Assay ID Hs03043790_g1, # 4331182), and CYP2E1 (Life Technologies, Assay ID Hs00559368_m1, # 4453320).

    Techniques: Quantitative RT-PCR

    Immunostaining of CYP2D6 in SH-SY5Y cells after β-NF and EtOH treatment. Representative immunofluorescence images of each experimental condition. Column 1 represents mitochondrial stain (red), column 2 represents ER-Green Fluorescent Protein (GFP) stain (green), and column 3 reports the CYP2D6 staining (blue). Column 4 represents the merge between CYP2D6 and mitochondria channel, column 5 represents the merge between CYP2D6 and ER-GFP, and column 6 represents the merge of the three channels. R Values: Pearson’s correlation coefficient. N/A: Not determined. Scale bars: 20 µm (control and EtOH) and 40 µm (β-NF).

    Journal: International Journal of Molecular Sciences

    Article Title: β-Naphtoflavone and Ethanol Induce Cytochrome P450 and Protect towards MPP+ Toxicity in Human Neuroblastoma SH-SY5Y Cells

    doi: 10.3390/ijms19113369

    Figure Lengend Snippet: Immunostaining of CYP2D6 in SH-SY5Y cells after β-NF and EtOH treatment. Representative immunofluorescence images of each experimental condition. Column 1 represents mitochondrial stain (red), column 2 represents ER-Green Fluorescent Protein (GFP) stain (green), and column 3 reports the CYP2D6 staining (blue). Column 4 represents the merge between CYP2D6 and mitochondria channel, column 5 represents the merge between CYP2D6 and ER-GFP, and column 6 represents the merge of the three channels. R Values: Pearson’s correlation coefficient. N/A: Not determined. Scale bars: 20 µm (control and EtOH) and 40 µm (β-NF).

    Article Snippet: The following probes for CYP isoforms were used, CYP1A1 (Life Technologies, Assay ID Hs01054797_g1, # 4331182), CYP2D6 (Life Technologies, Assay ID Hs03043790_g1, # 4331182), and CYP2E1 (Life Technologies, Assay ID Hs00559368_m1, # 4453320).

    Techniques: Immunostaining, Immunofluorescence, Staining

    CpG island of CYP2E1 and CYP2D6 gene. Note: ( A ) CYP2E1 gene CpG island; ( B ) CYP2D6 gene CpG island.

    Journal: International Journal of Environmental Research and Public Health

    Article Title: Correlation of CpG Island Methylation of the Cytochrome P450 2E1/2D6 Genes with Liver Injury Induced by Anti-Tuberculosis Drugs: A Nested Case-Control Study

    doi: 10.3390/ijerph13080776

    Figure Lengend Snippet: CpG island of CYP2E1 and CYP2D6 gene. Note: ( A ) CYP2E1 gene CpG island; ( B ) CYP2D6 gene CpG island.

    Article Snippet: Analysis of Gene Polymorphism PCR primers for the CYP2E1 and CYP2D6 genes were selected based on previous studies [ , , ] and synthesized by Invitrogen Beijing.

    Techniques:

    CYP2D6*17 (1023C > T) TaqMan Assay Genotype Results. (Panels A and B) show the scatter plots for TaqMan assays C__2222771_40 (original assay) and C__2222771_A0 (alternative assay). Numbered subjects correspond to cases 1–9 and 21–23 ( Tables 1 and 2 ). Cases 1–9 were identified as 1023 T/T with the original assay (panel A), but genotyped accurately as 1023 C/T with the alternative assay (panel B). Additional samples of known genotypes were analyzed as references to allow cluster formation in the scatter plots; none of these controls carry a CYP2D6*4 .

    Journal: Scientific Reports

    Article Title: SNP genotyping using TaqMan® technology: the CYP2D6*17 assay conundrum

    doi: 10.1038/srep09257

    Figure Lengend Snippet: CYP2D6*17 (1023C > T) TaqMan Assay Genotype Results. (Panels A and B) show the scatter plots for TaqMan assays C__2222771_40 (original assay) and C__2222771_A0 (alternative assay). Numbered subjects correspond to cases 1–9 and 21–23 ( Tables 1 and 2 ). Cases 1–9 were identified as 1023 T/T with the original assay (panel A), but genotyped accurately as 1023 C/T with the alternative assay (panel B). Additional samples of known genotypes were analyzed as references to allow cluster formation in the scatter plots; none of these controls carry a CYP2D6*4 .

    Article Snippet: Partial resequencing of the duplicated CYP2D6*4 gene of cases 21–23 also determined absence of the 974C > A, 984A > G and 997C > G SNP; cases 24 and 25 were not available for sequence analysis.

    Techniques: TaqMan Assay

    CYP2D6*4 subvariants. The top panel provides details about the SNVs including rs numbers, positions relative to the numbering system on which the allele nomenclature is based (M33388) and the widely accepted CYP2D6*1 reference sequence AY545216, the nature of the SNV, and sequence context. The presence of the intron 1 conversion event is characterized by multiple SNPs (rs1080995, rs267608284, rs108996, rs74644586, rs75276289, rs28695233, rs149744965, rs56011157). The middle ‘checkerboard’ panel shows SNVs relative to the AY545216 reference. Variation is highlighted by colored boxes. Gray boxes denote sequence errors in M33388. For general information, sequence differences to Genome Build GRCh37 that harbors a CYP2D6*2 , is shown. Lines *4A through *4N depict a graphical representation of the P450 Nomenclature Database 20 for these alleles. Note that *4A-K lacks any annotations for intronic regions. Sequence variations found in cases 1 and 21 that match with CYP2D6*4A and *4D are highlighted in red and blue, respectively. Intronic SNPs in the cases are shown in light red and blue as these are not represented in the nomenclature-defined variants. Cases were sequenced between the SNPs shown in yellow. The bottom panels provide location of SNV regarding to exon and intron and consequence of SNV.

    Journal: Scientific Reports

    Article Title: SNP genotyping using TaqMan® technology: the CYP2D6*17 assay conundrum

    doi: 10.1038/srep09257

    Figure Lengend Snippet: CYP2D6*4 subvariants. The top panel provides details about the SNVs including rs numbers, positions relative to the numbering system on which the allele nomenclature is based (M33388) and the widely accepted CYP2D6*1 reference sequence AY545216, the nature of the SNV, and sequence context. The presence of the intron 1 conversion event is characterized by multiple SNPs (rs1080995, rs267608284, rs108996, rs74644586, rs75276289, rs28695233, rs149744965, rs56011157). The middle ‘checkerboard’ panel shows SNVs relative to the AY545216 reference. Variation is highlighted by colored boxes. Gray boxes denote sequence errors in M33388. For general information, sequence differences to Genome Build GRCh37 that harbors a CYP2D6*2 , is shown. Lines *4A through *4N depict a graphical representation of the P450 Nomenclature Database 20 for these alleles. Note that *4A-K lacks any annotations for intronic regions. Sequence variations found in cases 1 and 21 that match with CYP2D6*4A and *4D are highlighted in red and blue, respectively. Intronic SNPs in the cases are shown in light red and blue as these are not represented in the nomenclature-defined variants. Cases were sequenced between the SNPs shown in yellow. The bottom panels provide location of SNV regarding to exon and intron and consequence of SNV.

    Article Snippet: Partial resequencing of the duplicated CYP2D6*4 gene of cases 21–23 also determined absence of the 974C > A, 984A > G and 997C > G SNP; cases 24 and 25 were not available for sequence analysis.

    Techniques: Sequencing

    Real-time amplification for the original and alternate CYP2D6*17 TaqMan assays. Fluorescent signals detected for individuals with CYP2D6*4/*4 and CYP2D6*1/*1 genotypes. (Panels A and C) represents the original TaqMan assay (C__222771_40), (panels B and D) the alternate assay C__2222771_A0). The green curves indicate amplification from the ‘C’ allele (wild-type/reference, 1023C or CYP2D6*17 ) while the green curve represents the ‘T’ allele (variant, 1023 T, CYP2D6*17 or other variant haplotype containing this SNP).

    Journal: Scientific Reports

    Article Title: SNP genotyping using TaqMan® technology: the CYP2D6*17 assay conundrum

    doi: 10.1038/srep09257

    Figure Lengend Snippet: Real-time amplification for the original and alternate CYP2D6*17 TaqMan assays. Fluorescent signals detected for individuals with CYP2D6*4/*4 and CYP2D6*1/*1 genotypes. (Panels A and C) represents the original TaqMan assay (C__222771_40), (panels B and D) the alternate assay C__2222771_A0). The green curves indicate amplification from the ‘C’ allele (wild-type/reference, 1023C or CYP2D6*17 ) while the green curve represents the ‘T’ allele (variant, 1023 T, CYP2D6*17 or other variant haplotype containing this SNP).

    Article Snippet: Partial resequencing of the duplicated CYP2D6*4 gene of cases 21–23 also determined absence of the 974C > A, 984A > G and 997C > G SNP; cases 24 and 25 were not available for sequence analysis.

    Techniques: Amplification, TaqMan Assay, Variant Assay

    CYP2D6*17 (1023C > T) TaqMan Assay Genotype Results. (Panels A and B) show the scatter plots for TaqMan assays C__2222771_40 (original assay) and C__2222771_A0 (alternative assay). Numbered subjects correspond to cases 1–9 and 21–23 ( Tables 1 and 2 ). Cases 1–9 were identified as 1023 T/T with the original assay (panel A), but genotyped accurately as 1023 C/T with the alternative assay (panel B). Additional samples of known genotypes were analyzed as references to allow cluster formation in the scatter plots; none of these controls carry a CYP2D6*4 .

    Journal: Scientific Reports

    Article Title: SNP genotyping using TaqMan® technology: the CYP2D6*17 assay conundrum

    doi: 10.1038/srep09257

    Figure Lengend Snippet: CYP2D6*17 (1023C > T) TaqMan Assay Genotype Results. (Panels A and B) show the scatter plots for TaqMan assays C__2222771_40 (original assay) and C__2222771_A0 (alternative assay). Numbered subjects correspond to cases 1–9 and 21–23 ( Tables 1 and 2 ). Cases 1–9 were identified as 1023 T/T with the original assay (panel A), but genotyped accurately as 1023 C/T with the alternative assay (panel B). Additional samples of known genotypes were analyzed as references to allow cluster formation in the scatter plots; none of these controls carry a CYP2D6*4 .

    Article Snippet: Cases 21–25 carried a CYP2D6*4 gene duplication; four were typed heterozygous for 1023C > T similar to cases 19 and 20, while one was homozygous.

    Techniques: TaqMan Assay

    CYP2D6*4 subvariants. The top panel provides details about the SNVs including rs numbers, positions relative to the numbering system on which the allele nomenclature is based (M33388) and the widely accepted CYP2D6*1 reference sequence AY545216, the nature of the SNV, and sequence context. The presence of the intron 1 conversion event is characterized by multiple SNPs (rs1080995, rs267608284, rs108996, rs74644586, rs75276289, rs28695233, rs149744965, rs56011157). The middle ‘checkerboard’ panel shows SNVs relative to the AY545216 reference. Variation is highlighted by colored boxes. Gray boxes denote sequence errors in M33388. For general information, sequence differences to Genome Build GRCh37 that harbors a CYP2D6*2 , is shown. Lines *4A through *4N depict a graphical representation of the P450 Nomenclature Database 20 for these alleles. Note that *4A-K lacks any annotations for intronic regions. Sequence variations found in cases 1 and 21 that match with CYP2D6*4A and *4D are highlighted in red and blue, respectively. Intronic SNPs in the cases are shown in light red and blue as these are not represented in the nomenclature-defined variants. Cases were sequenced between the SNPs shown in yellow. The bottom panels provide location of SNV regarding to exon and intron and consequence of SNV.

    Journal: Scientific Reports

    Article Title: SNP genotyping using TaqMan® technology: the CYP2D6*17 assay conundrum

    doi: 10.1038/srep09257

    Figure Lengend Snippet: CYP2D6*4 subvariants. The top panel provides details about the SNVs including rs numbers, positions relative to the numbering system on which the allele nomenclature is based (M33388) and the widely accepted CYP2D6*1 reference sequence AY545216, the nature of the SNV, and sequence context. The presence of the intron 1 conversion event is characterized by multiple SNPs (rs1080995, rs267608284, rs108996, rs74644586, rs75276289, rs28695233, rs149744965, rs56011157). The middle ‘checkerboard’ panel shows SNVs relative to the AY545216 reference. Variation is highlighted by colored boxes. Gray boxes denote sequence errors in M33388. For general information, sequence differences to Genome Build GRCh37 that harbors a CYP2D6*2 , is shown. Lines *4A through *4N depict a graphical representation of the P450 Nomenclature Database 20 for these alleles. Note that *4A-K lacks any annotations for intronic regions. Sequence variations found in cases 1 and 21 that match with CYP2D6*4A and *4D are highlighted in red and blue, respectively. Intronic SNPs in the cases are shown in light red and blue as these are not represented in the nomenclature-defined variants. Cases were sequenced between the SNPs shown in yellow. The bottom panels provide location of SNV regarding to exon and intron and consequence of SNV.

    Article Snippet: Cases 21–25 carried a CYP2D6*4 gene duplication; four were typed heterozygous for 1023C > T similar to cases 19 and 20, while one was homozygous.

    Techniques: Sequencing

    Real-time amplification for the original and alternate CYP2D6*17 TaqMan assays. Fluorescent signals detected for individuals with CYP2D6*4/*4 and CYP2D6*1/*1 genotypes. (Panels A and C) represents the original TaqMan assay (C__222771_40), (panels B and D) the alternate assay C__2222771_A0). The green curves indicate amplification from the ‘C’ allele (wild-type/reference, 1023C or CYP2D6*17 ) while the green curve represents the ‘T’ allele (variant, 1023 T, CYP2D6*17 or other variant haplotype containing this SNP).

    Journal: Scientific Reports

    Article Title: SNP genotyping using TaqMan® technology: the CYP2D6*17 assay conundrum

    doi: 10.1038/srep09257

    Figure Lengend Snippet: Real-time amplification for the original and alternate CYP2D6*17 TaqMan assays. Fluorescent signals detected for individuals with CYP2D6*4/*4 and CYP2D6*1/*1 genotypes. (Panels A and C) represents the original TaqMan assay (C__222771_40), (panels B and D) the alternate assay C__2222771_A0). The green curves indicate amplification from the ‘C’ allele (wild-type/reference, 1023C or CYP2D6*17 ) while the green curve represents the ‘T’ allele (variant, 1023 T, CYP2D6*17 or other variant haplotype containing this SNP).

    Article Snippet: Cases 21–25 carried a CYP2D6*4 gene duplication; four were typed heterozygous for 1023C > T similar to cases 19 and 20, while one was homozygous.

    Techniques: Amplification, TaqMan Assay, Variant Assay

    PCR fragment analysis. The PCR products for amplicons F–J were used in fragment analysis on the Agilent Technologies DNA 7500 Kit to show the presence or absence of the CYP2D7 gene, CYP2D6 genes, or recombinant allele. Fragments A–E are not shown as these were primarily useful for the generation of amplicons used in allele-specific primer extension assay reactions and sequencing reactions. Genotypes tested are shown at the top of each lane. The ladder in the left column shows the size in base pairs of the molecular weight marker. The uppermost purple band in all sample lanes represents a marker depicting the maximum size that the kit can detect reliably. (a) PCR fragment F analysis detects CYP2D7 exon 9 through the region 3′ of rep 6. The * 68 +* 4-like and * 4N +* 4-like arrangements did not produce a product because the * 4-like allele did not have the exon 9 conversion present. The sample containing the * 5 /* 5 genotype and those with single * 36 , * 61 , and * 63 alleles generated an amplicon. The size of the product is consistent with the presence of the CYP2D7 -derived spacer sequence which was confirmed by DNA sequencing. (b) PCR fragment G analysis that detects CYP2D7/CYP2D6 genes. Only samples with * 13 and * 16 generated an amplicon. (c) PCR fragment H analysis detects DNA spanning from the CYP2D7 promoter to CYP2D6 promoter regions. The presence or absence of 1.6 kb spacer sequence can be visualized based on the amplicon size. Genotype * 5 /* 5 lacks the CYP2D6 promoter region, so does not generate an amplicon. Genotype * 13 +* 2A /* 5 lacks the 1.6 kb spacer sequence, so the amplicon is smaller than the products generated by the other genotypes tested. (d) PCR fragment I analysis generates an amplicon spanning from CYP2D8 3′region to a unique region in CYP2D6 intron 3. The * 13 +* 2A -containing sample generated an amplicon because CYP2D7 was absent. No amplicon was detected in * 16 samples because the crossover region in * 16 occurs after exon 7, which eliminates the reverse primer binding site for the amplicons. Normal gene arrangements and typical duplication arrangements do not generate a product because the size of the amplicons is too great. (e) PCR fragment J analysis generates amplicons spanning from the CYP2D8 3′ region to the CYP2D6 -specific trailing sequences. Amplicons are generated for the * 13 +* 2A -containing and * 16 -containing samples because CYP2D7 is absent in these samples. Normal gene arrangements and typical duplication arrangements do not generate a product because the size of the amplicons is too great.

    Journal: Pharmacogenetics and genomics

    Article Title: CYP2D6: novel genomic structures and alleles

    doi: 10.1097/FPC.0b013e3283317b95

    Figure Lengend Snippet: PCR fragment analysis. The PCR products for amplicons F–J were used in fragment analysis on the Agilent Technologies DNA 7500 Kit to show the presence or absence of the CYP2D7 gene, CYP2D6 genes, or recombinant allele. Fragments A–E are not shown as these were primarily useful for the generation of amplicons used in allele-specific primer extension assay reactions and sequencing reactions. Genotypes tested are shown at the top of each lane. The ladder in the left column shows the size in base pairs of the molecular weight marker. The uppermost purple band in all sample lanes represents a marker depicting the maximum size that the kit can detect reliably. (a) PCR fragment F analysis detects CYP2D7 exon 9 through the region 3′ of rep 6. The * 68 +* 4-like and * 4N +* 4-like arrangements did not produce a product because the * 4-like allele did not have the exon 9 conversion present. The sample containing the * 5 /* 5 genotype and those with single * 36 , * 61 , and * 63 alleles generated an amplicon. The size of the product is consistent with the presence of the CYP2D7 -derived spacer sequence which was confirmed by DNA sequencing. (b) PCR fragment G analysis that detects CYP2D7/CYP2D6 genes. Only samples with * 13 and * 16 generated an amplicon. (c) PCR fragment H analysis detects DNA spanning from the CYP2D7 promoter to CYP2D6 promoter regions. The presence or absence of 1.6 kb spacer sequence can be visualized based on the amplicon size. Genotype * 5 /* 5 lacks the CYP2D6 promoter region, so does not generate an amplicon. Genotype * 13 +* 2A /* 5 lacks the 1.6 kb spacer sequence, so the amplicon is smaller than the products generated by the other genotypes tested. (d) PCR fragment I analysis generates an amplicon spanning from CYP2D8 3′region to a unique region in CYP2D6 intron 3. The * 13 +* 2A -containing sample generated an amplicon because CYP2D7 was absent. No amplicon was detected in * 16 samples because the crossover region in * 16 occurs after exon 7, which eliminates the reverse primer binding site for the amplicons. Normal gene arrangements and typical duplication arrangements do not generate a product because the size of the amplicons is too great. (e) PCR fragment J analysis generates amplicons spanning from the CYP2D8 3′ region to the CYP2D6 -specific trailing sequences. Amplicons are generated for the * 13 +* 2A -containing and * 16 -containing samples because CYP2D7 is absent in these samples. Normal gene arrangements and typical duplication arrangements do not generate a product because the size of the amplicons is too great.

    Article Snippet: The gene copy number was determined on samples with duplication signals by the CYP2D6 ASPE kit by using the TaqMan CYP-2D6 Gene Copy Number Assay (Applied Biosystems, Foster City, California, USA, Assay ID Hs00010001_cn) per product literature.

    Techniques: Polymerase Chain Reaction, Recombinant, Primer Extension Assay, Sequencing, Molecular Weight, Marker, Generated, Amplification, Derivative Assay, DNA Sequencing, Binding Assay

    ]. Chromosomes are shown in black. Blocks in the chromosomes depict CYP2D6 and CYP2D7 . (a) Interchromosomal recombination occurs between two independent homologous chromosomes during meiosis. (b) Intrachromosomal recombination occurs between sister chromatids of the same chromosome. (c) Intrachromatidic recombination involves recombination between low copy repeats on the same chromatid. Episomal DNA circles can undergo reinsertion after DNA replication at the excision site or at different loci on the same chromatid.

    Journal: Pharmacogenetics and genomics

    Article Title: CYP2D6: novel genomic structures and alleles

    doi: 10.1097/FPC.0b013e3283317b95

    Figure Lengend Snippet: ]. Chromosomes are shown in black. Blocks in the chromosomes depict CYP2D6 and CYP2D7 . (a) Interchromosomal recombination occurs between two independent homologous chromosomes during meiosis. (b) Intrachromosomal recombination occurs between sister chromatids of the same chromosome. (c) Intrachromatidic recombination involves recombination between low copy repeats on the same chromatid. Episomal DNA circles can undergo reinsertion after DNA replication at the excision site or at different loci on the same chromatid.

    Article Snippet: The gene copy number was determined on samples with duplication signals by the CYP2D6 ASPE kit by using the TaqMan CYP-2D6 Gene Copy Number Assay (Applied Biosystems, Foster City, California, USA, Assay ID Hs00010001_cn) per product literature.

    Techniques:

    The cytochrome P450 ( CYP ) 2D6 activity scores associated with tamoxifen metabolites and metabolic ratios. Activity scores for CYP2D6 were associated with steady‐state plasma concentrations of ( a ) endoxifen and ( b ) 4‐hydroxytamoxifen (4‐OH‐Tam). The CYP2D6 activity scores were also associated with metabolic ratios of ( c ) endoxifen/tamoxifen, ( d ) 4‐OH‐Tam/tamoxifen, ( e ) endoxifen/4‐OH‐Tam, and ( f ) endoxifen/N‐desmethyltamoxifen ( N ‐dm‐Tam). Genotype‐phenotype associations with P

    Journal: Clinical and Translational Science

    Article Title: Cytochrome P450 Genetic Variation Associated with Tamoxifen Biotransformation in American Indian and Alaska Native People

    doi: 10.1111/cts.12542

    Figure Lengend Snippet: The cytochrome P450 ( CYP ) 2D6 activity scores associated with tamoxifen metabolites and metabolic ratios. Activity scores for CYP2D6 were associated with steady‐state plasma concentrations of ( a ) endoxifen and ( b ) 4‐hydroxytamoxifen (4‐OH‐Tam). The CYP2D6 activity scores were also associated with metabolic ratios of ( c ) endoxifen/tamoxifen, ( d ) 4‐OH‐Tam/tamoxifen, ( e ) endoxifen/4‐OH‐Tam, and ( f ) endoxifen/N‐desmethyltamoxifen ( N ‐dm‐Tam). Genotype‐phenotype associations with P

    Article Snippet: Supplementary Table S2 CYP2D6 allele frequencies in SCF population by Fluidigm assay (n = 726 chromosomes) Click here for additional data file.

    Techniques: Activity Assay

    Heat maps of the DNA methylation profiles of the representative 6 CYP and 2 control genes by cluster analysis. The top of the map indicates samples from 20 normal adult livers (L1 to L81, NLA and NL2), 1 fetal liver (NLF), 1 adult small intestine (NSI), and 3 hepatoma cell lines. The right of the map indicates the target ID and gene name ( CYP1A2 , CYP1B1 , CYP2C9 , CYP2C19 , CYP2D6 , CYP3A4 , ACTB , and BMP4 ) examined for individual CpG sites. The representative methylation profiles of individual genes were selected by excluding profiles showing the similar patterns each other. Therefore, the hierarchical similarity tree was not shown in the map. Higher DNA methylation levels are shown in red , while lower DNA methylation levels are shown in black

    Journal: Clinical Epigenetics

    Article Title: Analysis of DNA methylation landscape reveals the roles of DNA methylation in the regulation of drug metabolizing enzymes

    doi: 10.1186/s13148-015-0136-7

    Figure Lengend Snippet: Heat maps of the DNA methylation profiles of the representative 6 CYP and 2 control genes by cluster analysis. The top of the map indicates samples from 20 normal adult livers (L1 to L81, NLA and NL2), 1 fetal liver (NLF), 1 adult small intestine (NSI), and 3 hepatoma cell lines. The right of the map indicates the target ID and gene name ( CYP1A2 , CYP1B1 , CYP2C9 , CYP2C19 , CYP2D6 , CYP3A4 , ACTB , and BMP4 ) examined for individual CpG sites. The representative methylation profiles of individual genes were selected by excluding profiles showing the similar patterns each other. Therefore, the hierarchical similarity tree was not shown in the map. Higher DNA methylation levels are shown in red , while lower DNA methylation levels are shown in black

    Article Snippet: In contrast, DMEs with highly variable DNA methylation (including CYP1A2 , CYP2C19 , and CYP2D6 ) catalyze the metabolism of many xenobiotics, including drugs used in the clinical setting.

    Techniques: DNA Methylation Assay, Methylation

    Representative results of correlation analysis. A significant inverse correlation between DNA methylation level ( β value) and mRNA expression level was detected for the CYP2C19 ( a ) and GSTA4 ( c ) genes ( p

    Journal: Clinical Epigenetics

    Article Title: Analysis of DNA methylation landscape reveals the roles of DNA methylation in the regulation of drug metabolizing enzymes

    doi: 10.1186/s13148-015-0136-7

    Figure Lengend Snippet: Representative results of correlation analysis. A significant inverse correlation between DNA methylation level ( β value) and mRNA expression level was detected for the CYP2C19 ( a ) and GSTA4 ( c ) genes ( p

    Article Snippet: In contrast, DMEs with highly variable DNA methylation (including CYP1A2 , CYP2C19 , and CYP2D6 ) catalyze the metabolism of many xenobiotics, including drugs used in the clinical setting.

    Techniques: DNA Methylation Assay, Expressing