Journal: BMC Cancer
Article Title: Relevance of cyclin D1b expression and CCND1 polymorphism in the pathogenesis of multiple myeloma and mantle cell lymphoma
Figure Lengend Snippet: Cyclin D1a and cyclin D1b protein expression . A. Whole cell extracts were obtained from CD19+ cells of MCL patients, separated on SDS-PAGE and sequentially immunoblotted with anti-cyclin D1a (sc-718) or anti-cyclin D1b (R3) Abs. For loading controls, blots were also revealed with anti-β-tubulin Ab (sc-9104, not shown). B. Proteins were obtained from MCL cell lines and analyzed as described in A except that we used DCS-6 anti-cyclin D1 Ab which recognizes both isoforms.
Article Snippet: The DCS-6 monoclonal anti-cyclin D1 antibody (Ab, Pharmingen BD Biosciences n°556070) is directed against the entire cyclin D1 molecule and recognizes both isoforms; the sc-718 anti-cyclin D1 Ab (Santa Cruz Biotechnologies) is directed against the C-terminal part of the protein and is specific for cyclin D1a, the R3 Ab is directed against the C-terminal part of cyclin D1b and is specific for this isoform [ ].
Techniques: Expressing, SDS Page