cyclin d1 Search Results


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  • 93
    Addgene inc plasmids 11181
    Plasmids 11181, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasmids 11181/product/Addgene inc
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    96
    Santa Cruz Biotechnology anti cyclin d1
    Anti Cyclin D1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cyclin d1/product/Santa Cruz Biotechnology
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    93
    Addgene inc pcmv ccnd1
    miR-193b is methylated in cancer samples and has inverse expression pattern compared to <t>CCND1</t> . (A) miR-193b methylation in clinical samples (BPH, PC, and CRPC) was assessed by MethylMiner-qPCR. miR-193b and CCND1 expressions were studied by miRNA and mRNA microarray (B) in prostate cancer cell lines and (C) xenograft samples. Spearman correlation coefficiencies are given. BPH, benign prostate hyperplasias; CRPC, castration-resistant tumors; PC, prostate cancer.
    Pcmv Ccnd1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcmv ccnd1/product/Addgene inc
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    96
    Cell Signaling Technology Inc 217 ccnd1
    miR-193b is methylated in cancer samples and has inverse expression pattern compared to <t>CCND1</t> . (A) miR-193b methylation in clinical samples (BPH, PC, and CRPC) was assessed by MethylMiner-qPCR. miR-193b and CCND1 expressions were studied by miRNA and mRNA microarray (B) in prostate cancer cell lines and (C) xenograft samples. Spearman correlation coefficiencies are given. BPH, benign prostate hyperplasias; CRPC, castration-resistant tumors; PC, prostate cancer.
    217 Ccnd1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/217 ccnd1/product/Cell Signaling Technology Inc
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    93
    Cell Signaling Technology Inc p cyclin d1
    Repeated low-dose NaAsO 2 exposure induced phosphorylated protein expression of GSK-3β/cyclinD1, p21 and p27 was regulated by AKT. Protein expression was detected by the Western blot analysis. (A) Images are the representative results of three separate experiments. Quantitative graphs ( n = 3) showed the relative intensity of the target proteins compare with β-actin, including p -AKT (B) , p -GSK-3β (C) , total <t>cyclin</t> <t>D1</t> (D) , p -cyclin D1 (E) , p -p21 (F) , p -p27 (G) , total p21 (H) and total p27 (I) . NaAsO 2 exposure induced increased expression of p -AKT, p -GSK-3β, total cyclin D1, p -p21, and p -p27, but decreased expression of p -cyclin D1, total p21, and total p27. Treatment of MK2206 significantly reversed the expression of all of the above proteins. Significant difference was defined as p less than 0.05. a, vs. the corresponding 0 μM group; b, vs. the corresponding 0.05 μM group; c, vs. the MK2206(-) group of the same NaAsO 2 concentration.
    P Cyclin D1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p cyclin d1/product/Cell Signaling Technology Inc
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    Image Search Results


    miR-193b is methylated in cancer samples and has inverse expression pattern compared to CCND1 . (A) miR-193b methylation in clinical samples (BPH, PC, and CRPC) was assessed by MethylMiner-qPCR. miR-193b and CCND1 expressions were studied by miRNA and mRNA microarray (B) in prostate cancer cell lines and (C) xenograft samples. Spearman correlation coefficiencies are given. BPH, benign prostate hyperplasias; CRPC, castration-resistant tumors; PC, prostate cancer.

    Journal: Cancer Medicine

    Article Title: Epigenetically altered miR-193b targets cyclin D1 in prostate cancer

    doi: 10.1002/cam4.486

    Figure Lengend Snippet: miR-193b is methylated in cancer samples and has inverse expression pattern compared to CCND1 . (A) miR-193b methylation in clinical samples (BPH, PC, and CRPC) was assessed by MethylMiner-qPCR. miR-193b and CCND1 expressions were studied by miRNA and mRNA microarray (B) in prostate cancer cell lines and (C) xenograft samples. Spearman correlation coefficiencies are given. BPH, benign prostate hyperplasias; CRPC, castration-resistant tumors; PC, prostate cancer.

    Article Snippet: For the Cyclin D1 rescue and cell cycle experiment, 22Rv1 cells were cotransfected with 10 μ g of plasmid, pCMV-CCND1 lacking a 3’UTR (Plasmid 19927; Addgene, Cambridge, MA), or the control plasmid pCMV6-AC-GFP (OriGene Technologies, Rockville, MD) along with either pre-miR-193b or pre-miR-scramble at a final concentration of 10 nmol/L, using jetPRIME transfection reagent (Polyplus-transfection).

    Techniques: Methylation, Expressing, Microarray

    miR-193b overexpression reduces the expression of CCND1 , the level of Cyclin D1 protein and phosphorylation level of retinoblastoma (RB). 22Rv1 prostate cancer cells were transfected with pre-miR-193b or pre-miR-control. (A) Expression levels of CCND1 mRNA was measured using q-RT-PCR. Western blot analysis was used to detect protein expression of (B) Cyclin D1 from total proteins and (C) phosphorylation level of RB protein from nuclear protein fraction. Actin and fibrillarin antibodies were used as loading controls.

    Journal: Cancer Medicine

    Article Title: Epigenetically altered miR-193b targets cyclin D1 in prostate cancer

    doi: 10.1002/cam4.486

    Figure Lengend Snippet: miR-193b overexpression reduces the expression of CCND1 , the level of Cyclin D1 protein and phosphorylation level of retinoblastoma (RB). 22Rv1 prostate cancer cells were transfected with pre-miR-193b or pre-miR-control. (A) Expression levels of CCND1 mRNA was measured using q-RT-PCR. Western blot analysis was used to detect protein expression of (B) Cyclin D1 from total proteins and (C) phosphorylation level of RB protein from nuclear protein fraction. Actin and fibrillarin antibodies were used as loading controls.

    Article Snippet: For the Cyclin D1 rescue and cell cycle experiment, 22Rv1 cells were cotransfected with 10 μ g of plasmid, pCMV-CCND1 lacking a 3’UTR (Plasmid 19927; Addgene, Cambridge, MA), or the control plasmid pCMV6-AC-GFP (OriGene Technologies, Rockville, MD) along with either pre-miR-193b or pre-miR-scramble at a final concentration of 10 nmol/L, using jetPRIME transfection reagent (Polyplus-transfection).

    Techniques: Over Expression, Expressing, Transfection, Reverse Transcription Polymerase Chain Reaction, Western Blot

    miR-193b targets CCND1 3’UTR. (A) Luciferase experiment was performed in 22Rv1 cells cotransfected with pSGG-plasmid containing CCND1 3’UTR, Renilla luciferase plasmid, and pre-miR-193b or pre-miR-control. Values were normalized against Renilla luciferase activity. The means of four experiments ±SEM are shown. * P -value <0.05 (B) Western blot analysis of pRB in 22Rv1 cells transfected with pCMV-CCND1 plasmid lacking CCND1 3’UTR together with miR-scramble or miR-193b. Fibrillarin antibody was used as loading control for nuclear proteins.

    Journal: Cancer Medicine

    Article Title: Epigenetically altered miR-193b targets cyclin D1 in prostate cancer

    doi: 10.1002/cam4.486

    Figure Lengend Snippet: miR-193b targets CCND1 3’UTR. (A) Luciferase experiment was performed in 22Rv1 cells cotransfected with pSGG-plasmid containing CCND1 3’UTR, Renilla luciferase plasmid, and pre-miR-193b or pre-miR-control. Values were normalized against Renilla luciferase activity. The means of four experiments ±SEM are shown. * P -value <0.05 (B) Western blot analysis of pRB in 22Rv1 cells transfected with pCMV-CCND1 plasmid lacking CCND1 3’UTR together with miR-scramble or miR-193b. Fibrillarin antibody was used as loading control for nuclear proteins.

    Article Snippet: For the Cyclin D1 rescue and cell cycle experiment, 22Rv1 cells were cotransfected with 10 μ g of plasmid, pCMV-CCND1 lacking a 3’UTR (Plasmid 19927; Addgene, Cambridge, MA), or the control plasmid pCMV6-AC-GFP (OriGene Technologies, Rockville, MD) along with either pre-miR-193b or pre-miR-scramble at a final concentration of 10 nmol/L, using jetPRIME transfection reagent (Polyplus-transfection).

    Techniques: Luciferase, Plasmid Preparation, Activity Assay, Western Blot, Transfection

    Association of clinicopathological variables with  Cyclin D1  expression

    Journal: Cancer Medicine

    Article Title: Epigenetically altered miR-193b targets cyclin D1 in prostate cancer

    doi: 10.1002/cam4.486

    Figure Lengend Snippet: Association of clinicopathological variables with Cyclin D1 expression

    Article Snippet: For the Cyclin D1 rescue and cell cycle experiment, 22Rv1 cells were cotransfected with 10 μ g of plasmid, pCMV-CCND1 lacking a 3’UTR (Plasmid 19927; Addgene, Cambridge, MA), or the control plasmid pCMV6-AC-GFP (OriGene Technologies, Rockville, MD) along with either pre-miR-193b or pre-miR-scramble at a final concentration of 10 nmol/L, using jetPRIME transfection reagent (Polyplus-transfection).

    Techniques: Expressing

    Repeated low-dose NaAsO 2 exposure induced phosphorylated protein expression of GSK-3β/cyclinD1, p21 and p27 was regulated by AKT. Protein expression was detected by the Western blot analysis. (A) Images are the representative results of three separate experiments. Quantitative graphs ( n = 3) showed the relative intensity of the target proteins compare with β-actin, including p -AKT (B) , p -GSK-3β (C) , total cyclin D1 (D) , p -cyclin D1 (E) , p -p21 (F) , p -p27 (G) , total p21 (H) and total p27 (I) . NaAsO 2 exposure induced increased expression of p -AKT, p -GSK-3β, total cyclin D1, p -p21, and p -p27, but decreased expression of p -cyclin D1, total p21, and total p27. Treatment of MK2206 significantly reversed the expression of all of the above proteins. Significant difference was defined as p less than 0.05. a, vs. the corresponding 0 μM group; b, vs. the corresponding 0.05 μM group; c, vs. the MK2206(-) group of the same NaAsO 2 concentration.

    Journal: Frontiers in Pharmacology

    Article Title: Akt Regulated Phosphorylation of GSK-3β/Cyclin D1, p21 and p27 Contributes to Cell Proliferation Through Cell Cycle Progression From G1 to S/G2M Phase in Low-Dose Arsenite Exposed HaCat Cells

    doi: 10.3389/fphar.2019.01176

    Figure Lengend Snippet: Repeated low-dose NaAsO 2 exposure induced phosphorylated protein expression of GSK-3β/cyclinD1, p21 and p27 was regulated by AKT. Protein expression was detected by the Western blot analysis. (A) Images are the representative results of three separate experiments. Quantitative graphs ( n = 3) showed the relative intensity of the target proteins compare with β-actin, including p -AKT (B) , p -GSK-3β (C) , total cyclin D1 (D) , p -cyclin D1 (E) , p -p21 (F) , p -p27 (G) , total p21 (H) and total p27 (I) . NaAsO 2 exposure induced increased expression of p -AKT, p -GSK-3β, total cyclin D1, p -p21, and p -p27, but decreased expression of p -cyclin D1, total p21, and total p27. Treatment of MK2206 significantly reversed the expression of all of the above proteins. Significant difference was defined as p less than 0.05. a, vs. the corresponding 0 μM group; b, vs. the corresponding 0.05 μM group; c, vs. the MK2206(-) group of the same NaAsO 2 concentration.

    Article Snippet: Antibodies against p -Akt (Ser473, #9271), Akt (#4685S), GSK-3β (#5676), p -cyclin D1 (Thr286, #3300), p21 (#2947), p27 (#3686), and MMP9 (#13667) were purchased from Cell Signaling Technology, USA.

    Techniques: Expressing, Western Blot, Concentration Assay