Journal: Molecules

Article Title: Structural Optimization and Improving Antitumor Potential of Moreollic Acid from Gamboge

doi: 10.3390/molecules27020482

Figure Lengend Snippet: TH12-10 blocks S phase entry. ( A ) Immunoblot analysis of Cyclin D1, CDK4, CDK6, and p-Rb in SW620 and DLD1 cells treated with indicated concentrations of TH12-10 for 48 h. ( B ) Densitometry analysis of indicated proteins as described in ( A ) was performed using Image J software. ( C ) Flow cytometric analysis for cell-cycle distribution in DLD1 cells treated with TH12-10 or vehicle and then with nocodazole for 48 h. ( D ) Quantitative analysis of DLD1 cells in G1 phase as described in ( C ). All data are presented as means ± SD of three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001. N.S., not significant. Differences were tested using one-way ANOVA ( B ) or the unpaired t-test with Welch’s correction ( D ).

Article Snippet: Antibodies used for the immunoblot analysis in this study were as follows: Cyclin D1 (55506T, Cell Signaling Technology, Danvers, MA, USA), CDK4 (ab199728, Abcam, Cambridge, UK), CDK6 (13331T, Cell Signaling Technology, Danvers, MA, USA), phospho-Rb (Ser807/811) (8516S, Cell Signaling Technology, Danvers, MA, USA), and β-actin (sc-47778, Santa Cruz Biotechnology, CA, USA)

Techniques: Western Blot, Software

Journal: Molecules

Article Title: Structural Optimization and Improving Antitumor Potential of Moreollic Acid from Gamboge

doi: 10.3390/molecules27020482

Figure Lengend Snippet: The contribution of TH12-10 to the cell cycle. ( A ) Western blotting shows Cyclin D1, CDK4, CDK6, and p-Rb levels in NCM460 cells treated with TH12-10 at indicated concentrations for 48 h. ( B ) Densitometry analysis of indicated proteins as described in ( A ) was conducted and presented. ( C ) Flow cytometric analysis for cell-cycle distribution in NCM460 cells treated with TH12-10 or vehicle and then with nocodazole for a total of 48 h. ( D ) Quantitative analysis of NCM460 cells in G1 phase as described in ( C ). All data are presented as means ± SD of three independent experiments. N.S., not significant. Differences were tested using one-way ANOVA ( B ) or the unpaired t-test with Welch’s correction ( D ).

Article Snippet: Antibodies used for the immunoblot analysis in this study were as follows: Cyclin D1 (55506T, Cell Signaling Technology, Danvers, MA, USA), CDK4 (ab199728, Abcam, Cambridge, UK), CDK6 (13331T, Cell Signaling Technology, Danvers, MA, USA), phospho-Rb (Ser807/811) (8516S, Cell Signaling Technology, Danvers, MA, USA), and β-actin (sc-47778, Santa Cruz Biotechnology, CA, USA)

Techniques: Western Blot