Journal: The Journal of Neuroscience
Article Title: Glutamate Activates AMPA Receptor Conductance in the Developing Schwann Cells of the Mammalian Peripheral Nerves
Figure Lengend Snippet: Only SCs without response to glutamate are involved in myelination of axons. A , Example of a current response to a series of depolarizing voltage steps applied from V hold = −80 mV (blue dotted line) with an increment of +10 mV for a type 1 cell filled with Alexa Fluor-568 ( D ) and stained for MBP ( G , J ). The currents are overlaid and the capacitive transients are blanked for clarity. Red arrow indicates the time point when a voltage step started. B , As in A but for a type 2 cell. C , As in A but for a type 4 cell. Note the difference between the scale bars in A – C . D – F , Type 1, type 2, and type 4 cells filled with Alexa Fluor-568 during patch-clamp recordings in the sciatic nerve slice (red, single confocal plane). Arrowheads point to the processes of the cells. G – I , Postrecording immunohistochemistry for MBP (green; Cy5 in G and H or Alexa Fluor-488 in I ). The confocal plane and the position of the arrowheads are the same as in D – F . J – L , Overlay of red and green channels shown in D – I . Note that the recorded cells of type 1 and type 2 are negative for MBP, whereas the recorded cell of type 4 is positive for MBP. Gray double-ended arrow indicates the orientation of the axons in the nerve.
Article Snippet: The following secondary antibodies were used: goat anti-rat Alexa Fluor-488 (Invitrogen, 1:500) and goat anti-rat biotin-SP-conjugated (1:200; Dianova), followed by application of streptavidin-Cy5 (1:200; Biomol/Rockland), goat anti-rabbit Alexa Fluor-488 (Invitrogen, 1:500), and goat anti-rabbit Cy5 (1:200; Biomol/Rockland).
Techniques: Staining, Patch Clamp, Immunohistochemistry