cy3-conjugated streptavidin Search Results


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  • 99
    Thermo Fisher cy 3 conjugated streptavidin
    Cy 3 Conjugated Streptavidin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cy 3 conjugated streptavidin/product/Thermo Fisher
    Average 99 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
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    96
    Millipore cy3 conjugated streptavidin
    Chemotherapy treatment increases ABCC2 expression in ovarian cancer cells. A . qRT-PCR for ABCC2 expression. Ovarian cancer cells treated with LD 50 CBP for 72 hr. OVCAR-5 cells also treated in presence or absence of HA oligomers (HYA oligo, 250 μg/ml). Relative gene expression was determined by calibration against no CBP control and normalized to the house keeping gene β-actin using the 2 -∆∆CT method. Data represents mean ± SEM from 3 independent experiments performed in triplicate. B . OVCAR-5 treated with LD 50 CBP for 72 hr. Fixed cells were incubated with biotinylated HABP and ABCC2 mouse monoclonal antibody and visualized with <t>Cy3-conjugated</t> <t>streptavidin</t> (red) and FITC-conjugated donkey anti-mouse immunoglobulins (green) respectively. Nuclei are counterstained with DAPI dye (blue).
    Cy3 Conjugated Streptavidin, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 417 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    BioLegend cy3 conjugated streptavidin
    Chemotherapy treatment increases ABCC2 expression in ovarian cancer cells. A . qRT-PCR for ABCC2 expression. Ovarian cancer cells treated with LD 50 CBP for 72 hr. OVCAR-5 cells also treated in presence or absence of HA oligomers (HYA oligo, 250 μg/ml). Relative gene expression was determined by calibration against no CBP control and normalized to the house keeping gene β-actin using the 2 -∆∆CT method. Data represents mean ± SEM from 3 independent experiments performed in triplicate. B . OVCAR-5 treated with LD 50 CBP for 72 hr. Fixed cells were incubated with biotinylated HABP and ABCC2 mouse monoclonal antibody and visualized with <t>Cy3-conjugated</t> <t>streptavidin</t> (red) and FITC-conjugated donkey anti-mouse immunoglobulins (green) respectively. Nuclei are counterstained with DAPI dye (blue).
    Cy3 Conjugated Streptavidin, supplied by BioLegend, used in various techniques. Bioz Stars score: 94/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cy3 conjugated streptavidin/product/BioLegend
    Average 94 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
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    99
    Thermo Fisher cy3 conjugated streptavidin
    In vivo imaging of fibrinogen leakage in 5XFAD mice. a , Fluorescent Aβ probe methoxy-X04-positive amyloid plaque in a cortical section from a 3-month-old 5XFAD mouse co-labeled for fibrinogen (red) and CD11b (green). Scale bar: 20 μm. n = 4 mice. Representative images are shown. b , In vivo two-photon imaging of cortex from Thy1- YFP:5XFAD mice at 11 months of age shows Alexa594-conjugated fibrinogen (administered intravenously) (red), a methoxy-X04-positive Aβ plaque (blue), and dystrophic neurites (green, swollen green structures). Arrowheads indicate fibrinogen (red) surrounding a Aβ plaque (blue) at areas of neuritic loss. Arrows indicate fibrinogen extravasation at areas of dystrophic neurites (yellow) in proximity to a plaque (blue). Scale bars: 10 μm. (n = 4 Thy1- YFP : 5XFAD mice and n = 2 Thy1- YFP mice. Representative images are shown. c , 5B8 in vivo target engagement in the brain of 5XFAD mice. Brain sections of 5XFAD i.p.-injected with biotinylated 5B8 were stained with <t>Cy3-streptavidin</t> (red), anti-fibrinogen (green), and methoxy-X04-positive Aβ plaque (blue). Orthogonal views of the y/z and x/z planes show localization of fibrinogen and 5B8 antibody around amyloid plaques. Scale bar, 10 μm. n =3 mice Representative images are shown.
    Cy3 Conjugated Streptavidin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 292 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cy3 conjugated streptavidin/product/Thermo Fisher
    Average 99 stars, based on 292 article reviews
    Price from $9.99 to $1999.99
    cy3 conjugated streptavidin - by Bioz Stars, 2020-07
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    89
    Thermo Fisher streptavidin cy3 conjugate
    In vivo imaging of fibrinogen leakage in 5XFAD mice. a , Fluorescent Aβ probe methoxy-X04-positive amyloid plaque in a cortical section from a 3-month-old 5XFAD mouse co-labeled for fibrinogen (red) and CD11b (green). Scale bar: 20 μm. n = 4 mice. Representative images are shown. b , In vivo two-photon imaging of cortex from Thy1- YFP:5XFAD mice at 11 months of age shows Alexa594-conjugated fibrinogen (administered intravenously) (red), a methoxy-X04-positive Aβ plaque (blue), and dystrophic neurites (green, swollen green structures). Arrowheads indicate fibrinogen (red) surrounding a Aβ plaque (blue) at areas of neuritic loss. Arrows indicate fibrinogen extravasation at areas of dystrophic neurites (yellow) in proximity to a plaque (blue). Scale bars: 10 μm. (n = 4 Thy1- YFP : 5XFAD mice and n = 2 Thy1- YFP mice. Representative images are shown. c , 5B8 in vivo target engagement in the brain of 5XFAD mice. Brain sections of 5XFAD i.p.-injected with biotinylated 5B8 were stained with <t>Cy3-streptavidin</t> (red), anti-fibrinogen (green), and methoxy-X04-positive Aβ plaque (blue). Orthogonal views of the y/z and x/z planes show localization of fibrinogen and 5B8 antibody around amyloid plaques. Scale bar, 10 μm. n =3 mice Representative images are shown.
    Streptavidin Cy3 Conjugate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 77 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    GE Healthcare cy3 conjugated to streptavidin
    The S-phase fraction of cells increases with loss of an extra 3p21 copy . Living cells of the MCHs #3/KH3 and Δ3/KH39 were incubated with BrdU in final concentration 30 μg/ml during 1 hour followed by fixation with methanol: acetic acid (3:1). FISH with biotin-labeled 3p21-specific RP6-123i13 probe and centromere-specific probe was performed to analyze the #3/KH3 and the Δ3/KH39 respectively. The probe signal was developed using <t>Cy3-conjugated</t> <t>streptavidin</t> (red), while FITC-conjugated anti BrdU antibodies (Roche Molecular Biochemicals Mannheim, Germany) identified cells in S-phase (green). Percentage of S-phase cells in subpopulations of cells with different 3p21 copy numbers in the #3/KH39 and with different chr3 copy number (counted by number of centromeres) in the Δ3/KH39 was calculated in more than 100 nuclei. The calculation was repeated three times when number of cells allowed. The average values and standard deviations are shown.
    Cy3 Conjugated To Streptavidin, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Cedarlane cy3 conjugated streptavidin
    The S-phase fraction of cells increases with loss of an extra 3p21 copy . Living cells of the MCHs #3/KH3 and Δ3/KH39 were incubated with BrdU in final concentration 30 μg/ml during 1 hour followed by fixation with methanol: acetic acid (3:1). FISH with biotin-labeled 3p21-specific RP6-123i13 probe and centromere-specific probe was performed to analyze the #3/KH3 and the Δ3/KH39 respectively. The probe signal was developed using <t>Cy3-conjugated</t> <t>streptavidin</t> (red), while FITC-conjugated anti BrdU antibodies (Roche Molecular Biochemicals Mannheim, Germany) identified cells in S-phase (green). Percentage of S-phase cells in subpopulations of cells with different 3p21 copy numbers in the #3/KH39 and with different chr3 copy number (counted by number of centromeres) in the Δ3/KH39 was calculated in more than 100 nuclei. The calculation was repeated three times when number of cells allowed. The average values and standard deviations are shown.
    Cy3 Conjugated Streptavidin, supplied by Cedarlane, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Roche cy3 conjugated streptavidin
    The S-phase fraction of cells increases with loss of an extra 3p21 copy . Living cells of the MCHs #3/KH3 and Δ3/KH39 were incubated with BrdU in final concentration 30 μg/ml during 1 hour followed by fixation with methanol: acetic acid (3:1). FISH with biotin-labeled 3p21-specific RP6-123i13 probe and centromere-specific probe was performed to analyze the #3/KH3 and the Δ3/KH39 respectively. The probe signal was developed using <t>Cy3-conjugated</t> <t>streptavidin</t> (red), while FITC-conjugated anti BrdU antibodies (Roche Molecular Biochemicals Mannheim, Germany) identified cells in S-phase (green). Percentage of S-phase cells in subpopulations of cells with different 3p21 copy numbers in the #3/KH39 and with different chr3 copy number (counted by number of centromeres) in the Δ3/KH39 was calculated in more than 100 nuclei. The calculation was repeated three times when number of cells allowed. The average values and standard deviations are shown.
    Cy3 Conjugated Streptavidin, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cy3 conjugated streptavidin/product/Roche
    Average 92 stars, based on 20 article reviews
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    90
    Roche streptavidin conjugated to cy3
    The S-phase fraction of cells increases with loss of an extra 3p21 copy . Living cells of the MCHs #3/KH3 and Δ3/KH39 were incubated with BrdU in final concentration 30 μg/ml during 1 hour followed by fixation with methanol: acetic acid (3:1). FISH with biotin-labeled 3p21-specific RP6-123i13 probe and centromere-specific probe was performed to analyze the #3/KH3 and the Δ3/KH39 respectively. The probe signal was developed using <t>Cy3-conjugated</t> <t>streptavidin</t> (red), while FITC-conjugated anti BrdU antibodies (Roche Molecular Biochemicals Mannheim, Germany) identified cells in S-phase (green). Percentage of S-phase cells in subpopulations of cells with different 3p21 copy numbers in the #3/KH39 and with different chr3 copy number (counted by number of centromeres) in the Δ3/KH39 was calculated in more than 100 nuclei. The calculation was repeated three times when number of cells allowed. The average values and standard deviations are shown.
    Streptavidin Conjugated To Cy3, supplied by Roche, used in various techniques. Bioz Stars score: 90/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Cytocell streptavidin conjugated to cy3
    The S-phase fraction of cells increases with loss of an extra 3p21 copy . Living cells of the MCHs #3/KH3 and Δ3/KH39 were incubated with BrdU in final concentration 30 μg/ml during 1 hour followed by fixation with methanol: acetic acid (3:1). FISH with biotin-labeled 3p21-specific RP6-123i13 probe and centromere-specific probe was performed to analyze the #3/KH3 and the Δ3/KH39 respectively. The probe signal was developed using <t>Cy3-conjugated</t> <t>streptavidin</t> (red), while FITC-conjugated anti BrdU antibodies (Roche Molecular Biochemicals Mannheim, Germany) identified cells in S-phase (green). Percentage of S-phase cells in subpopulations of cells with different 3p21 copy numbers in the #3/KH39 and with different chr3 copy number (counted by number of centromeres) in the Δ3/KH39 was calculated in more than 100 nuclei. The calculation was repeated three times when number of cells allowed. The average values and standard deviations are shown.
    Streptavidin Conjugated To Cy3, supplied by Cytocell, used in various techniques. Bioz Stars score: 88/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin conjugated to cy3/product/Cytocell
    Average 88 stars, based on 16 article reviews
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    Image Search Results


    Chemotherapy treatment increases ABCC2 expression in ovarian cancer cells. A . qRT-PCR for ABCC2 expression. Ovarian cancer cells treated with LD 50 CBP for 72 hr. OVCAR-5 cells also treated in presence or absence of HA oligomers (HYA oligo, 250 μg/ml). Relative gene expression was determined by calibration against no CBP control and normalized to the house keeping gene β-actin using the 2 -∆∆CT method. Data represents mean ± SEM from 3 independent experiments performed in triplicate. B . OVCAR-5 treated with LD 50 CBP for 72 hr. Fixed cells were incubated with biotinylated HABP and ABCC2 mouse monoclonal antibody and visualized with Cy3-conjugated streptavidin (red) and FITC-conjugated donkey anti-mouse immunoglobulins (green) respectively. Nuclei are counterstained with DAPI dye (blue).

    Journal: BMC Cancer

    Article Title: Chemotherapy-induced hyaluronan production: a novel chemoresistance mechanism in ovarian cancer

    doi: 10.1186/1471-2407-13-476

    Figure Lengend Snippet: Chemotherapy treatment increases ABCC2 expression in ovarian cancer cells. A . qRT-PCR for ABCC2 expression. Ovarian cancer cells treated with LD 50 CBP for 72 hr. OVCAR-5 cells also treated in presence or absence of HA oligomers (HYA oligo, 250 μg/ml). Relative gene expression was determined by calibration against no CBP control and normalized to the house keeping gene β-actin using the 2 -∆∆CT method. Data represents mean ± SEM from 3 independent experiments performed in triplicate. B . OVCAR-5 treated with LD 50 CBP for 72 hr. Fixed cells were incubated with biotinylated HABP and ABCC2 mouse monoclonal antibody and visualized with Cy3-conjugated streptavidin (red) and FITC-conjugated donkey anti-mouse immunoglobulins (green) respectively. Nuclei are counterstained with DAPI dye (blue).

    Article Snippet: Negative controls included only Cy3-conjugated streptavidin and FITC-conjugated AffiniPure donkey anti-mouse and also Streptomyces hyaluronidase (30 min RT, 10 U/ml, Sigma-Aldrich) treatment for the HA staining.

    Techniques: Expressing, Quantitative RT-PCR, Incubation

    Vasohibin suppresses tumor growth and tumor angiogenesis. ( A ) The synthesis of vasohibin protein in LLC cells. Cell extracts were prepared from mock or vasohibin transfectants (Vh-bulk) for Western blotting. Clone 16 and clone 19 were vasohibin-producing clones. ( B ) Effect of vasohibin on the proliferation of LLC cells in vitro. Proliferation of mock transfectants, vasohibin transfectants, clone 16, and clone 19 was determined. ( C ) Effect of secreted vasohibin from LLC cells on the migration of HUVECs. Mock or vasohibin transfectants were plated on the lower compartment of a modified Boyden chamber and the migration of HUVECs toward the lower chamber of the Transwell insert was analyzed. Values are expressed as mean ± SD of 4 samples. ( D ) Effect of vasohibin gene transfection on the growth of LLC cells in vivo. BDF1 mice were inoculated intradermally with LLC cells. Tumor volume was determined consecutively. ( E ) Effect of vasohibin gene transfection on tumor angiogenesis. Paraffin sections were prepared from tumors for the immunostaining of CD31; sections obtained on day 8 after inoculation are shown. Visualization with a DAKO LSAB+/HRP kit is shown at left, and that with streptavidin-Cy3 conjugate on the right. Yellow lines trace vascular lumens. Scale bars: 50 μm. ( F ) Quantitative analysis of tumor vascular area. Total vascular area per field was determined using NIH Image and compared. Values are expressed as mean ± SD of 6 random fields. * P

    Journal: Journal of Clinical Investigation

    Article Title: Vasohibin as an endothelium-derived negative feedback regulator of angiogenesis

    doi: 10.1172/JCI200421152

    Figure Lengend Snippet: Vasohibin suppresses tumor growth and tumor angiogenesis. ( A ) The synthesis of vasohibin protein in LLC cells. Cell extracts were prepared from mock or vasohibin transfectants (Vh-bulk) for Western blotting. Clone 16 and clone 19 were vasohibin-producing clones. ( B ) Effect of vasohibin on the proliferation of LLC cells in vitro. Proliferation of mock transfectants, vasohibin transfectants, clone 16, and clone 19 was determined. ( C ) Effect of secreted vasohibin from LLC cells on the migration of HUVECs. Mock or vasohibin transfectants were plated on the lower compartment of a modified Boyden chamber and the migration of HUVECs toward the lower chamber of the Transwell insert was analyzed. Values are expressed as mean ± SD of 4 samples. ( D ) Effect of vasohibin gene transfection on the growth of LLC cells in vivo. BDF1 mice were inoculated intradermally with LLC cells. Tumor volume was determined consecutively. ( E ) Effect of vasohibin gene transfection on tumor angiogenesis. Paraffin sections were prepared from tumors for the immunostaining of CD31; sections obtained on day 8 after inoculation are shown. Visualization with a DAKO LSAB+/HRP kit is shown at left, and that with streptavidin-Cy3 conjugate on the right. Yellow lines trace vascular lumens. Scale bars: 50 μm. ( F ) Quantitative analysis of tumor vascular area. Total vascular area per field was determined using NIH Image and compared. Values are expressed as mean ± SD of 6 random fields. * P

    Article Snippet: Labeling was visualized using a streptavidin-Cy3 conjugate (Sigma-Aldrich) at a dilution of 1:100.

    Techniques: Western Blot, Clone Assay, In Vitro, Migration, Modification, Transfection, In Vivo, Mouse Assay, Immunostaining

    In situ hybridization to a pachytene cell from the line double monosomic for H ch ac and 6H ch S. Double FISH signals were observed using H. chilense genomic DNA detected with streptavidin–Cy3 (magenta) and a telomere repeat sequence probe detected with FITC (green). Blue DAPI staining shows wheat chromosomes. The acrocentric chromosome H ch ac and the 6H ch S chromosome pair along one of their distal parts. The centromeric part of the 6H ch S is indicated by an arrow.

    Journal: Journal of Experimental Botany

    Article Title: Fertility of CMS wheat is restored by two Rf loci located on a recombined acrocentric chromosome

    doi: 10.1093/jxb/eru388

    Figure Lengend Snippet: In situ hybridization to a pachytene cell from the line double monosomic for H ch ac and 6H ch S. Double FISH signals were observed using H. chilense genomic DNA detected with streptavidin–Cy3 (magenta) and a telomere repeat sequence probe detected with FITC (green). Blue DAPI staining shows wheat chromosomes. The acrocentric chromosome H ch ac and the 6H ch S chromosome pair along one of their distal parts. The centromeric part of the 6H ch S is indicated by an arrow.

    Article Snippet: Digoxigenin- and biotin-labelled probes were detected with antidigoxigenin-FITC (Roche Corporate) and streptavidin–Cy3 conjugates (Sigma, St Louis, MO, USA), respectively.

    Techniques: In Situ Hybridization, Fluorescence In Situ Hybridization, Sequencing, Staining

    Meiotic pairing analysis of the acrocentric chromosome with 1H ch and 1H ch S chromosomes. (A) In situ hybridization to a pachytene cell of the double monosomic H ch ac-1H ch line. Double FISH signals were observed using H. chilense genomic DNA detected with streptavidin–Cy3 (magenta) and a telomere repeat sequence probe detected with FITC (green). The acrocentric chromosome H ch ac is perfectly paired with the 1H ch chromosome except for one of its distal parts. (B) Meiotic anaphase I of a plant double monosomic for H ch ac and 1H ch S stained with carmine. Tension is observed (indicated by an arrow) between the short arm of the acrocentic chromosome and the 1H ch S as a result of their pairing.

    Journal: Journal of Experimental Botany

    Article Title: Fertility of CMS wheat is restored by two Rf loci located on a recombined acrocentric chromosome

    doi: 10.1093/jxb/eru388

    Figure Lengend Snippet: Meiotic pairing analysis of the acrocentric chromosome with 1H ch and 1H ch S chromosomes. (A) In situ hybridization to a pachytene cell of the double monosomic H ch ac-1H ch line. Double FISH signals were observed using H. chilense genomic DNA detected with streptavidin–Cy3 (magenta) and a telomere repeat sequence probe detected with FITC (green). The acrocentric chromosome H ch ac is perfectly paired with the 1H ch chromosome except for one of its distal parts. (B) Meiotic anaphase I of a plant double monosomic for H ch ac and 1H ch S stained with carmine. Tension is observed (indicated by an arrow) between the short arm of the acrocentic chromosome and the 1H ch S as a result of their pairing.

    Article Snippet: Digoxigenin- and biotin-labelled probes were detected with antidigoxigenin-FITC (Roche Corporate) and streptavidin–Cy3 conjugates (Sigma, St Louis, MO, USA), respectively.

    Techniques: In Situ Hybridization, Fluorescence In Situ Hybridization, Sequencing, Staining

    In situ hybridization to root-tip metaphase cells from restored line T749. (A) Double FISH signals using H. chilense genomic DNA detected with streptavidin–Cy3 (magenta) and a telomere repeat sequence probe detected with FITC (green). Blue DAPI staining shows wheat chromosomes. The acrocentric chromosome H ch ac displays magenta colour indicating its pure barley origin. Telomere sequences can be observed at both ends of the H ch ac chromosome. (B) FISH signal using the repetitive probe pAs1 detected with FITC. pAs1 shows a characteristic hybridization pattern in H. chilense that allows for the identification of the different chromosome arms. The H ch ac chromosome shows hybridization sites in both arms.

    Journal: Journal of Experimental Botany

    Article Title: Fertility of CMS wheat is restored by two Rf loci located on a recombined acrocentric chromosome

    doi: 10.1093/jxb/eru388

    Figure Lengend Snippet: In situ hybridization to root-tip metaphase cells from restored line T749. (A) Double FISH signals using H. chilense genomic DNA detected with streptavidin–Cy3 (magenta) and a telomere repeat sequence probe detected with FITC (green). Blue DAPI staining shows wheat chromosomes. The acrocentric chromosome H ch ac displays magenta colour indicating its pure barley origin. Telomere sequences can be observed at both ends of the H ch ac chromosome. (B) FISH signal using the repetitive probe pAs1 detected with FITC. pAs1 shows a characteristic hybridization pattern in H. chilense that allows for the identification of the different chromosome arms. The H ch ac chromosome shows hybridization sites in both arms.

    Article Snippet: Digoxigenin- and biotin-labelled probes were detected with antidigoxigenin-FITC (Roche Corporate) and streptavidin–Cy3 conjugates (Sigma, St Louis, MO, USA), respectively.

    Techniques: In Situ Hybridization, Fluorescence In Situ Hybridization, Sequencing, Staining, Hybridization

    In vivo imaging of fibrinogen leakage in 5XFAD mice. a , Fluorescent Aβ probe methoxy-X04-positive amyloid plaque in a cortical section from a 3-month-old 5XFAD mouse co-labeled for fibrinogen (red) and CD11b (green). Scale bar: 20 μm. n = 4 mice. Representative images are shown. b , In vivo two-photon imaging of cortex from Thy1- YFP:5XFAD mice at 11 months of age shows Alexa594-conjugated fibrinogen (administered intravenously) (red), a methoxy-X04-positive Aβ plaque (blue), and dystrophic neurites (green, swollen green structures). Arrowheads indicate fibrinogen (red) surrounding a Aβ plaque (blue) at areas of neuritic loss. Arrows indicate fibrinogen extravasation at areas of dystrophic neurites (yellow) in proximity to a plaque (blue). Scale bars: 10 μm. (n = 4 Thy1- YFP : 5XFAD mice and n = 2 Thy1- YFP mice. Representative images are shown. c , 5B8 in vivo target engagement in the brain of 5XFAD mice. Brain sections of 5XFAD i.p.-injected with biotinylated 5B8 were stained with Cy3-streptavidin (red), anti-fibrinogen (green), and methoxy-X04-positive Aβ plaque (blue). Orthogonal views of the y/z and x/z planes show localization of fibrinogen and 5B8 antibody around amyloid plaques. Scale bar, 10 μm. n =3 mice Representative images are shown.

    Journal: Nature immunology

    Article Title: Fibrin-targeting immunotherapy protects against neuroinflammation and neurodegeneration

    doi: 10.1038/s41590-018-0232-x

    Figure Lengend Snippet: In vivo imaging of fibrinogen leakage in 5XFAD mice. a , Fluorescent Aβ probe methoxy-X04-positive amyloid plaque in a cortical section from a 3-month-old 5XFAD mouse co-labeled for fibrinogen (red) and CD11b (green). Scale bar: 20 μm. n = 4 mice. Representative images are shown. b , In vivo two-photon imaging of cortex from Thy1- YFP:5XFAD mice at 11 months of age shows Alexa594-conjugated fibrinogen (administered intravenously) (red), a methoxy-X04-positive Aβ plaque (blue), and dystrophic neurites (green, swollen green structures). Arrowheads indicate fibrinogen (red) surrounding a Aβ plaque (blue) at areas of neuritic loss. Arrows indicate fibrinogen extravasation at areas of dystrophic neurites (yellow) in proximity to a plaque (blue). Scale bars: 10 μm. (n = 4 Thy1- YFP : 5XFAD mice and n = 2 Thy1- YFP mice. Representative images are shown. c , 5B8 in vivo target engagement in the brain of 5XFAD mice. Brain sections of 5XFAD i.p.-injected with biotinylated 5B8 were stained with Cy3-streptavidin (red), anti-fibrinogen (green), and methoxy-X04-positive Aβ plaque (blue). Orthogonal views of the y/z and x/z planes show localization of fibrinogen and 5B8 antibody around amyloid plaques. Scale bar, 10 μm. n =3 mice Representative images are shown.

    Article Snippet: Sections were fixed with 4% PFA for 10 min and biotinylated 5B8 was detected using Cy3-conjugated streptavidin (1:100; Invitrogen) for 30 min at 25 ˚C.

    Techniques: In Vivo Imaging, Mouse Assay, Labeling, In Vivo, Imaging, Injection, Staining

    The S-phase fraction of cells increases with loss of an extra 3p21 copy . Living cells of the MCHs #3/KH3 and Δ3/KH39 were incubated with BrdU in final concentration 30 μg/ml during 1 hour followed by fixation with methanol: acetic acid (3:1). FISH with biotin-labeled 3p21-specific RP6-123i13 probe and centromere-specific probe was performed to analyze the #3/KH3 and the Δ3/KH39 respectively. The probe signal was developed using Cy3-conjugated streptavidin (red), while FITC-conjugated anti BrdU antibodies (Roche Molecular Biochemicals Mannheim, Germany) identified cells in S-phase (green). Percentage of S-phase cells in subpopulations of cells with different 3p21 copy numbers in the #3/KH39 and with different chr3 copy number (counted by number of centromeres) in the Δ3/KH39 was calculated in more than 100 nuclei. The calculation was repeated three times when number of cells allowed. The average values and standard deviations are shown.

    Journal: BMC Cancer

    Article Title: Mandatory chromosomal segment balance in aneuploid tumor cells

    doi: 10.1186/1471-2407-7-21

    Figure Lengend Snippet: The S-phase fraction of cells increases with loss of an extra 3p21 copy . Living cells of the MCHs #3/KH3 and Δ3/KH39 were incubated with BrdU in final concentration 30 μg/ml during 1 hour followed by fixation with methanol: acetic acid (3:1). FISH with biotin-labeled 3p21-specific RP6-123i13 probe and centromere-specific probe was performed to analyze the #3/KH3 and the Δ3/KH39 respectively. The probe signal was developed using Cy3-conjugated streptavidin (red), while FITC-conjugated anti BrdU antibodies (Roche Molecular Biochemicals Mannheim, Germany) identified cells in S-phase (green). Percentage of S-phase cells in subpopulations of cells with different 3p21 copy numbers in the #3/KH39 and with different chr3 copy number (counted by number of centromeres) in the Δ3/KH39 was calculated in more than 100 nuclei. The calculation was repeated three times when number of cells allowed. The average values and standard deviations are shown.

    Article Snippet: The biotin-labeled probe hybridization was detected with Cy3 conjugated streptavidin (Amersham Biosciences, GE Healthcare Worldwide), the digoxigenin-labeled probe hybridization – using FITC conjugated anti-digoxigenin antibodies (Roche Molecular Biochemicals Mannheim).

    Techniques: Incubation, Concentration Assay, Fluorescence In Situ Hybridization, Labeling