Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
Article Title: Knockdown of MON1B Exerts Anti-Tumor Effects in Colon Cancer In Vitro
Figure Lengend Snippet: MON1B interference inhibited cell proliferation and migration of colon cancer cells via inhibiting the NF-κB pathway. ( A–C ) The mRNA levels of NF-κB p65, IκB, and CXCR-4 were measured by RT-qPCR. ( D ) The protein levels of NF-κB p65, IκB, and CXCR-4 were measured by Western blot. * P
Article Snippet: After that, the membrane was blocked with 5% non-fat dry milk for 1 h at 37°C and probed with specific primary antibodies overnight at 4°C, including: rabbit anti-MON1B (Novus, NBP1-92131, 1: 2000), anti-MMP2 (Abcam, ab92536, 1: 1000), anti-MMP9 (Abcam, ab38898, 1: 1000), anti-TIMP-2 (Abcam, ab180630, 1: 1000), anti-MTA-1 (Abcam, ab, 1: 1000), anti-NF-κB (Abcam, ab16502, 1: 1000), anti-IκB (Abcam, ab32518, 1: 1000), anti-CXCR-4 (Abcam, ab181020, 1: 1000), and anti-β-actin (Abcam, ab8227, 1: 2000).
Techniques: Migration, Quantitative RT-PCR, Western Blot