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  • 89
    Becton Dickinson cxcr 4
    IFN-γ down-regulates <t>CXCR-4</t> and CCR-5 expression by monocytic cells. (a) PBMCs (1 × 10 6 /ml) from 10 HIV-negative, six HIV-infected and 10 HIV-negative cord blood donors were incubated with IFN-γ (1 ng/ml) for 24 h, and the effects on cell surface CXCR-4 and CCR-5 expression by CD14 + monocytes were determined by flow cytometry. (b) PBMCs (1 × 10 6 /ml) from a representative HIV-negative individual were incubated with various concentrations of IFN-γ for 24 h followed by analysis of cell surface CXCR-4 and CCR-5 expression on CD14 + monocytes by flow cytometry. (c)Representative flow cytometry histograms of the effect of IFN-γ on the expression of CXCR-4 and CCR-5 by purified monocytes. CD14 + purified monocytes (2 × 10 5 /ml) isolated from a HIV-negative individual, an HIV-negative cord blood donor and an HIV-positive adult were stimulated with IFN-γ (1 ng/ml) for 24 h followed by determination of cell surface CXCR-4 and CCR-5 expression by CD14 + monocytes by flow cytometry. Shaded and unshaded histogram represent chemokine receptor expression in unstimulated and IFN-γ-stimulated cells, respectively.
    Cxcr 4, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 89/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher cxcr 4
    IFN-γ down-regulates <t>CXCR-4</t> and CCR-5 expression by monocytic cells. (a) PBMCs (1 × 10 6 /ml) from 10 HIV-negative, six HIV-infected and 10 HIV-negative cord blood donors were incubated with IFN-γ (1 ng/ml) for 24 h, and the effects on cell surface CXCR-4 and CCR-5 expression by CD14 + monocytes were determined by flow cytometry. (b) PBMCs (1 × 10 6 /ml) from a representative HIV-negative individual were incubated with various concentrations of IFN-γ for 24 h followed by analysis of cell surface CXCR-4 and CCR-5 expression on CD14 + monocytes by flow cytometry. (c)Representative flow cytometry histograms of the effect of IFN-γ on the expression of CXCR-4 and CCR-5 by purified monocytes. CD14 + purified monocytes (2 × 10 5 /ml) isolated from a HIV-negative individual, an HIV-negative cord blood donor and an HIV-positive adult were stimulated with IFN-γ (1 ng/ml) for 24 h followed by determination of cell surface CXCR-4 and CCR-5 expression by CD14 + monocytes by flow cytometry. Shaded and unshaded histogram represent chemokine receptor expression in unstimulated and IFN-γ-stimulated cells, respectively.
    Cxcr 4, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology cxcr 4 4g10
    IFN-γ down-regulates <t>CXCR-4</t> and CCR-5 expression by monocytic cells. (a) PBMCs (1 × 10 6 /ml) from 10 HIV-negative, six HIV-infected and 10 HIV-negative cord blood donors were incubated with IFN-γ (1 ng/ml) for 24 h, and the effects on cell surface CXCR-4 and CCR-5 expression by CD14 + monocytes were determined by flow cytometry. (b) PBMCs (1 × 10 6 /ml) from a representative HIV-negative individual were incubated with various concentrations of IFN-γ for 24 h followed by analysis of cell surface CXCR-4 and CCR-5 expression on CD14 + monocytes by flow cytometry. (c)Representative flow cytometry histograms of the effect of IFN-γ on the expression of CXCR-4 and CCR-5 by purified monocytes. CD14 + purified monocytes (2 × 10 5 /ml) isolated from a HIV-negative individual, an HIV-negative cord blood donor and an HIV-positive adult were stimulated with IFN-γ (1 ng/ml) for 24 h followed by determination of cell surface CXCR-4 and CCR-5 expression by CD14 + monocytes by flow cytometry. Shaded and unshaded histogram represent chemokine receptor expression in unstimulated and IFN-γ-stimulated cells, respectively.
    Cxcr 4 4g10, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    R&D Systems cxcr 4
    Zoledronic acid (ZOL) inhibits the chemotactic effect induced by SDF-1 on MDA-MB-231 cells – Partial reversion by geranylgeraniol (GGOH). Confluent MDA-MB-231 cells were treated for 18 h with 5 μ g ml −1 exoenzyme or 10 μ M FTI-277 or 10 μ M GGTI-298 or 1 μ M ZOL in the presence or absence of GGOH and farnesol (FOH) and seeded into the upper chamber of Transwell coated with Matrigel, and a gradient of SDF-1 (100 ng ml −1 ) was established by placing the chemokine in the lower chamber. Then the invasion was measured as described in Figure 2 . The inhibitory effect of ZOL was compared with that of C3 exoenzyme and of neutralising antibody against <t>CXCR-4.</t> Results are expressed as the percentage (as compared to control) of the mean±s.e.m. of five independent experiments. Significant difference from nontreatment control ( * ); from ZOL-treated cells ( # ) ( *,# P
    Cxcr 4, supplied by R&D Systems, used in various techniques. Bioz Stars score: 89/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam anti cxcr 4
    MON1B interference inhibited cell proliferation and migration of colon cancer cells via inhibiting the NF-κB pathway. ( A–C ) The mRNA levels of NF-κB p65, IκB, and <t>CXCR-4</t> were measured by RT-qPCR. ( D ) The protein levels of NF-κB p65, IκB, and CXCR-4 were measured by Western blot. * P
    Anti Cxcr 4, supplied by Abcam, used in various techniques. Bioz Stars score: 95/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology receptor cxcr 4
    MON1B interference inhibited cell proliferation and migration of colon cancer cells via inhibiting the NF-κB pathway. ( A–C ) The mRNA levels of NF-κB p65, IκB, and <t>CXCR-4</t> were measured by RT-qPCR. ( D ) The protein levels of NF-κB p65, IκB, and CXCR-4 were measured by Western blot. * P
    Receptor Cxcr 4, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam cxcr 4 antibody
    Flow cytometry showing C-X-C chemokine receptor type 4 <t>(CXCR-4)</t> surface expression in mesenchymal stem cells (MSCs) labelled with anti-CXCR-4 antibody, from a) young, b) adult control, and c) ovariectomized (OVX) rats. Young MSCs have the highest expression of CXCR-4 (87%), followed by MSCs from adult control (32%); MSCs from OVX rats (19%) had the lowest expression of CXCR-4 (n = 3). In a), the red histogram shows the secondary background control and the green histogram shows the CXCR-4 expression in young MSCs. In figures b) and c), the green histogram demonstrates the background expression and the red histogram shows the CXCR-4 expression. FITC, fluorescein isothiocyanate.
    Cxcr 4 Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher cxcr 4 pe cy7
    Flow cytometry showing C-X-C chemokine receptor type 4 <t>(CXCR-4)</t> surface expression in mesenchymal stem cells (MSCs) labelled with anti-CXCR-4 antibody, from a) young, b) adult control, and c) ovariectomized (OVX) rats. Young MSCs have the highest expression of CXCR-4 (87%), followed by MSCs from adult control (32%); MSCs from OVX rats (19%) had the lowest expression of CXCR-4 (n = 3). In a), the red histogram shows the secondary background control and the green histogram shows the CXCR-4 expression in young MSCs. In figures b) and c), the green histogram demonstrates the background expression and the red histogram shows the CXCR-4 expression. FITC, fluorescein isothiocyanate.
    Cxcr 4 Pe Cy7, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology cxcr 4
    Knockout of Notch3 reduces progenitor cells and worse cardiac function in post-MI. A and B. Western blot analysis of the expression of <t>CXCR-4</t> and SDF-1α expression revealed that the expression of CXCR4 was significantly reduced in the ischemic
    Cxcr 4, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 89/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    AnaSpec anti cxcr 4
    Chemokine receptor expression byB16 melanoma cell lines. Immunoblot analysis results demonstrate that B16-F1, B16α 4 +, and B16α 4 - express similar amounts of the chemokine receptors CCR-10, CCR-7, and <t>CXCR-4.</t> CXCR-3 expression was slightly
    Anti Cxcr 4, supplied by AnaSpec, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson anti cxcr 4
    Chemokine receptor expression byB16 melanoma cell lines. Immunoblot analysis results demonstrate that B16-F1, B16α 4 +, and B16α 4 - express similar amounts of the chemokine receptors CCR-10, CCR-7, and <t>CXCR-4.</t> CXCR-3 expression was slightly
    Anti Cxcr 4, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 88/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Pharmingen anti cxcr 4
    Northern blot analysis of <t>CXCR-4</t> and CCR-5 mRNA expression in freshly isolated thymocytes. Three thymocyte preparations from different subjects (lanes 1, 2 and 3) were analysed. The CXCR-4 and CCR-5 messages revealed in a representative freshly isolated PBL sample is also shown. Autoradiographs for CCR-5 were obtained after 5-day exposure.
    Anti Cxcr 4, supplied by Pharmingen, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam cxcr 4
    Northern blot analysis of <t>CXCR-4</t> and CCR-5 mRNA expression in freshly isolated thymocytes. Three thymocyte preparations from different subjects (lanes 1, 2 and 3) were analysed. The CXCR-4 and CCR-5 messages revealed in a representative freshly isolated PBL sample is also shown. Autoradiographs for CCR-5 were obtained after 5-day exposure.
    Cxcr 4, supplied by Abcam, used in various techniques. Bioz Stars score: 89/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam rabbit anti cxcr 4
    hsa-miR-9 regulates the expression of <t>CXCR-4</t>
    Rabbit Anti Cxcr 4, supplied by Abcam, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore cxcr 4
    hsa-miR-9 regulates the expression of <t>CXCR-4</t>
    Cxcr 4, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    R&D Systems cxcr 4 apc
    hsa-miR-9 regulates the expression of <t>CXCR-4</t>
    Cxcr 4 Apc, supplied by R&D Systems, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    R&D Systems mouse anti cxcr 4
    Differential distribution of Orai1 and TRPM7. A. Confocal images of migrating activated T cells stained for Orai1 (green) together with either CD44 (red) or <t>CXCR-4</t> (red). Yellow areas in the merge images indicate colocalization. Scale bar = 5 µm. The average correlation coefficients for the uropod (U, n = 21) and the leading-edge (L, n = 22) from 2 donors are shown in the right panel. B. Confocal images of migrating activated T cells that were fixed and stained for TRPM7 (green) together with either CD44 (red) or CXCR-4 (red). Correlation between two proteins is indicated by yellow areas in the merge images. Scale bar = 5 µm. The average correlation coefficients for the uropod (U, n = 29) and the leading-edge (L, n = 12) from 2 donors are shown in the right panel.
    Mouse Anti Cxcr 4, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson cxcr 4 pe
    EPO administration after myocardial infarction increased homing of EGFP + BMCs into ischaemic myocardium in GFP-transgenic mice. (A) Bar graph representing the percentage of myocardial EGFP + cell populations (subclassified by CD31, c-kit, <t>CXCR-4,</t> Sca-1) of infarcted control mice (white bar) or EPO-treated mice (black bar). All data represent mean ± S.E.M. ( n = 8). (B) Bar graph representing the fold-increase of EGFP + subpopulations in the hearts of infarcted control mice (white bar) or EPO-treated mice (black bar). The represented value is the ratio of the mean of control mice and the mean of EPO-treated mice.
    Cxcr 4 Pe, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 88/100, based on 132 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Capralogics protein cxcr 4
    EPO administration after myocardial infarction increased homing of EGFP + BMCs into ischaemic myocardium in GFP-transgenic mice. (A) Bar graph representing the percentage of myocardial EGFP + cell populations (subclassified by CD31, c-kit, <t>CXCR-4,</t> Sca-1) of infarcted control mice (white bar) or EPO-treated mice (black bar). All data represent mean ± S.E.M. ( n = 8). (B) Bar graph representing the fold-increase of EGFP + subpopulations in the hearts of infarcted control mice (white bar) or EPO-treated mice (black bar). The represented value is the ratio of the mean of control mice and the mean of EPO-treated mice.
    Protein Cxcr 4, supplied by Capralogics, used in various techniques. Bioz Stars score: 85/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad anti cxcr 4 mab
    EPO administration after myocardial infarction increased homing of EGFP + BMCs into ischaemic myocardium in GFP-transgenic mice. (A) Bar graph representing the percentage of myocardial EGFP + cell populations (subclassified by CD31, c-kit, <t>CXCR-4,</t> Sca-1) of infarcted control mice (white bar) or EPO-treated mice (black bar). All data represent mean ± S.E.M. ( n = 8). (B) Bar graph representing the fold-increase of EGFP + subpopulations in the hearts of infarcted control mice (white bar) or EPO-treated mice (black bar). The represented value is the ratio of the mean of control mice and the mean of EPO-treated mice.
    Anti Cxcr 4 Mab, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    R&D Systems anti cxcr 4 fitc
    EPO administration after myocardial infarction increased homing of EGFP + BMCs into ischaemic myocardium in GFP-transgenic mice. (A) Bar graph representing the percentage of myocardial EGFP + cell populations (subclassified by CD31, c-kit, <t>CXCR-4,</t> Sca-1) of infarcted control mice (white bar) or EPO-treated mice (black bar). All data represent mean ± S.E.M. ( n = 8). (B) Bar graph representing the fold-increase of EGFP + subpopulations in the hearts of infarcted control mice (white bar) or EPO-treated mice (black bar). The represented value is the ratio of the mean of control mice and the mean of EPO-treated mice.
    Anti Cxcr 4 Fitc, supplied by R&D Systems, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology cxc chemokine receptor cxcr 4
    EPO administration after myocardial infarction increased homing of EGFP + BMCs into ischaemic myocardium in GFP-transgenic mice. (A) Bar graph representing the percentage of myocardial EGFP + cell populations (subclassified by CD31, c-kit, <t>CXCR-4,</t> Sca-1) of infarcted control mice (white bar) or EPO-treated mice (black bar). All data represent mean ± S.E.M. ( n = 8). (B) Bar graph representing the fold-increase of EGFP + subpopulations in the hearts of infarcted control mice (white bar) or EPO-treated mice (black bar). The represented value is the ratio of the mean of control mice and the mean of EPO-treated mice.
    Cxc Chemokine Receptor Cxcr 4, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Capralogics anti cxcr 4
    EPO administration after myocardial infarction increased homing of EGFP + BMCs into ischaemic myocardium in GFP-transgenic mice. (A) Bar graph representing the percentage of myocardial EGFP + cell populations (subclassified by CD31, c-kit, <t>CXCR-4,</t> Sca-1) of infarcted control mice (white bar) or EPO-treated mice (black bar). All data represent mean ± S.E.M. ( n = 8). (B) Bar graph representing the fold-increase of EGFP + subpopulations in the hearts of infarcted control mice (white bar) or EPO-treated mice (black bar). The represented value is the ratio of the mean of control mice and the mean of EPO-treated mice.
    Anti Cxcr 4, supplied by Capralogics, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson cd184 cxcr 4 apc
    EPO administration after myocardial infarction increased homing of EGFP + BMCs into ischaemic myocardium in GFP-transgenic mice. (A) Bar graph representing the percentage of myocardial EGFP + cell populations (subclassified by CD31, c-kit, <t>CXCR-4,</t> Sca-1) of infarcted control mice (white bar) or EPO-treated mice (black bar). All data represent mean ± S.E.M. ( n = 8). (B) Bar graph representing the fold-increase of EGFP + subpopulations in the hearts of infarcted control mice (white bar) or EPO-treated mice (black bar). The represented value is the ratio of the mean of control mice and the mean of EPO-treated mice.
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    4Gene cxcr 4 gene
    EPO administration after myocardial infarction increased homing of EGFP + BMCs into ischaemic myocardium in GFP-transgenic mice. (A) Bar graph representing the percentage of myocardial EGFP + cell populations (subclassified by CD31, c-kit, <t>CXCR-4,</t> Sca-1) of infarcted control mice (white bar) or EPO-treated mice (black bar). All data represent mean ± S.E.M. ( n = 8). (B) Bar graph representing the fold-increase of EGFP + subpopulations in the hearts of infarcted control mice (white bar) or EPO-treated mice (black bar). The represented value is the ratio of the mean of control mice and the mean of EPO-treated mice.
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    Image Search Results


    IFN-γ down-regulates CXCR-4 and CCR-5 expression by monocytic cells. (a) PBMCs (1 × 10 6 /ml) from 10 HIV-negative, six HIV-infected and 10 HIV-negative cord blood donors were incubated with IFN-γ (1 ng/ml) for 24 h, and the effects on cell surface CXCR-4 and CCR-5 expression by CD14 + monocytes were determined by flow cytometry. (b) PBMCs (1 × 10 6 /ml) from a representative HIV-negative individual were incubated with various concentrations of IFN-γ for 24 h followed by analysis of cell surface CXCR-4 and CCR-5 expression on CD14 + monocytes by flow cytometry. (c)Representative flow cytometry histograms of the effect of IFN-γ on the expression of CXCR-4 and CCR-5 by purified monocytes. CD14 + purified monocytes (2 × 10 5 /ml) isolated from a HIV-negative individual, an HIV-negative cord blood donor and an HIV-positive adult were stimulated with IFN-γ (1 ng/ml) for 24 h followed by determination of cell surface CXCR-4 and CCR-5 expression by CD14 + monocytes by flow cytometry. Shaded and unshaded histogram represent chemokine receptor expression in unstimulated and IFN-γ-stimulated cells, respectively.

    Journal: Clinical and Experimental Immunology

    Article Title: Down-regulation of CXCR-4 and CCR-5 expression by interferon-? is associated with inhibition of chemotaxis and human immunodeficiency virus (HIV) replication but not HIV entry into human monocytes

    doi: 10.1111/j.1365-2249.2004.02495.x

    Figure Lengend Snippet: IFN-γ down-regulates CXCR-4 and CCR-5 expression by monocytic cells. (a) PBMCs (1 × 10 6 /ml) from 10 HIV-negative, six HIV-infected and 10 HIV-negative cord blood donors were incubated with IFN-γ (1 ng/ml) for 24 h, and the effects on cell surface CXCR-4 and CCR-5 expression by CD14 + monocytes were determined by flow cytometry. (b) PBMCs (1 × 10 6 /ml) from a representative HIV-negative individual were incubated with various concentrations of IFN-γ for 24 h followed by analysis of cell surface CXCR-4 and CCR-5 expression on CD14 + monocytes by flow cytometry. (c)Representative flow cytometry histograms of the effect of IFN-γ on the expression of CXCR-4 and CCR-5 by purified monocytes. CD14 + purified monocytes (2 × 10 5 /ml) isolated from a HIV-negative individual, an HIV-negative cord blood donor and an HIV-positive adult were stimulated with IFN-γ (1 ng/ml) for 24 h followed by determination of cell surface CXCR-4 and CCR-5 expression by CD14 + monocytes by flow cytometry. Shaded and unshaded histogram represent chemokine receptor expression in unstimulated and IFN-γ-stimulated cells, respectively.

    Article Snippet: The validity of comparisons of CXCR-4 and CCR-5 expression between different patients and populations was ensured with the use of Calbrite™ Beads (Becton Dickinson, Franklin Lakes, NJ, USA).

    Techniques: Expressing, Infection, Incubation, Flow Cytometry, Cytometry, Purification, Isolation

    The effect of IFN-γ on the transcription of mRNA encoding CXCR-4 and CCR-5. (a) Purified, CD14 + non-activated monocytes (5 × 10 5 /ml) isolated from HIV-negative adults were incubated with IFN-γ (1 ng/ml) for the indicated lengths of time. At various times following stimulation, RNA was isolated from these cells and subjected to RT-PCR analysis using primers specific for RNA encoding β-actin and either CXCR4 or CCR5. Amplified products were electrophoresed and analysed by densitometry using β-actin as an internal control. (b) Purified monocytes from three HIV-negative individuals and three cord blood donors were stimulated with either IL-2 or IFN-γ for 8 h. RNA isolated from these cells was subjected to semiquantitative RT-PCR analysis for CXCR-4 and CCR-5 expression. The results of one representative individual are shown (M = MW markers). The mean ratios of CXCR-4/β-actin and CCR-5/β-actin as determined by densitometric analysis from three individuals are shown.

    Journal: Clinical and Experimental Immunology

    Article Title: Down-regulation of CXCR-4 and CCR-5 expression by interferon-? is associated with inhibition of chemotaxis and human immunodeficiency virus (HIV) replication but not HIV entry into human monocytes

    doi: 10.1111/j.1365-2249.2004.02495.x

    Figure Lengend Snippet: The effect of IFN-γ on the transcription of mRNA encoding CXCR-4 and CCR-5. (a) Purified, CD14 + non-activated monocytes (5 × 10 5 /ml) isolated from HIV-negative adults were incubated with IFN-γ (1 ng/ml) for the indicated lengths of time. At various times following stimulation, RNA was isolated from these cells and subjected to RT-PCR analysis using primers specific for RNA encoding β-actin and either CXCR4 or CCR5. Amplified products were electrophoresed and analysed by densitometry using β-actin as an internal control. (b) Purified monocytes from three HIV-negative individuals and three cord blood donors were stimulated with either IL-2 or IFN-γ for 8 h. RNA isolated from these cells was subjected to semiquantitative RT-PCR analysis for CXCR-4 and CCR-5 expression. The results of one representative individual are shown (M = MW markers). The mean ratios of CXCR-4/β-actin and CCR-5/β-actin as determined by densitometric analysis from three individuals are shown.

    Article Snippet: The validity of comparisons of CXCR-4 and CCR-5 expression between different patients and populations was ensured with the use of Calbrite™ Beads (Becton Dickinson, Franklin Lakes, NJ, USA).

    Techniques: Purification, Isolation, Incubation, Reverse Transcription Polymerase Chain Reaction, Amplification, Expressing

    Zoledronic acid (ZOL) inhibits the chemotactic effect induced by SDF-1 on MDA-MB-231 cells – Partial reversion by geranylgeraniol (GGOH). Confluent MDA-MB-231 cells were treated for 18 h with 5 μ g ml −1 exoenzyme or 10 μ M FTI-277 or 10 μ M GGTI-298 or 1 μ M ZOL in the presence or absence of GGOH and farnesol (FOH) and seeded into the upper chamber of Transwell coated with Matrigel, and a gradient of SDF-1 (100 ng ml −1 ) was established by placing the chemokine in the lower chamber. Then the invasion was measured as described in Figure 2 . The inhibitory effect of ZOL was compared with that of C3 exoenzyme and of neutralising antibody against CXCR-4. Results are expressed as the percentage (as compared to control) of the mean±s.e.m. of five independent experiments. Significant difference from nontreatment control ( * ); from ZOL-treated cells ( # ) ( *,# P

    Journal: British Journal of Cancer

    Article Title: New insights into the actions of bisphosphonate zoledronic acid in breast cancer cells by dual RhoA-dependent and -independent effects

    doi: 10.1038/sj.bjc.6600925

    Figure Lengend Snippet: Zoledronic acid (ZOL) inhibits the chemotactic effect induced by SDF-1 on MDA-MB-231 cells – Partial reversion by geranylgeraniol (GGOH). Confluent MDA-MB-231 cells were treated for 18 h with 5 μ g ml −1 exoenzyme or 10 μ M FTI-277 or 10 μ M GGTI-298 or 1 μ M ZOL in the presence or absence of GGOH and farnesol (FOH) and seeded into the upper chamber of Transwell coated with Matrigel, and a gradient of SDF-1 (100 ng ml −1 ) was established by placing the chemokine in the lower chamber. Then the invasion was measured as described in Figure 2 . The inhibitory effect of ZOL was compared with that of C3 exoenzyme and of neutralising antibody against CXCR-4. Results are expressed as the percentage (as compared to control) of the mean±s.e.m. of five independent experiments. Significant difference from nontreatment control ( * ); from ZOL-treated cells ( # ) ( *,# P

    Article Snippet: Recently, a strong cell-surface expression of CXCR-4, the SDF-1 receptor, was described on the aggressive MDA-MB-231 breast-cancer cell line ( ).

    Techniques: Multiple Displacement Amplification

    MON1B interference inhibited cell proliferation and migration of colon cancer cells via inhibiting the NF-κB pathway. ( A–C ) The mRNA levels of NF-κB p65, IκB, and CXCR-4 were measured by RT-qPCR. ( D ) The protein levels of NF-κB p65, IκB, and CXCR-4 were measured by Western blot. * P

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: Knockdown of MON1B Exerts Anti-Tumor Effects in Colon Cancer In Vitro

    doi: 10.12659/MSM.911002

    Figure Lengend Snippet: MON1B interference inhibited cell proliferation and migration of colon cancer cells via inhibiting the NF-κB pathway. ( A–C ) The mRNA levels of NF-κB p65, IκB, and CXCR-4 were measured by RT-qPCR. ( D ) The protein levels of NF-κB p65, IκB, and CXCR-4 were measured by Western blot. * P

    Article Snippet: After that, the membrane was blocked with 5% non-fat dry milk for 1 h at 37°C and probed with specific primary antibodies overnight at 4°C, including: rabbit anti-MON1B (Novus, NBP1-92131, 1: 2000), anti-MMP2 (Abcam, ab92536, 1: 1000), anti-MMP9 (Abcam, ab38898, 1: 1000), anti-TIMP-2 (Abcam, ab180630, 1: 1000), anti-MTA-1 (Abcam, ab, 1: 1000), anti-NF-κB (Abcam, ab16502, 1: 1000), anti-IκB (Abcam, ab32518, 1: 1000), anti-CXCR-4 (Abcam, ab181020, 1: 1000), and anti-β-actin (Abcam, ab8227, 1: 2000).

    Techniques: Migration, Quantitative RT-PCR, Western Blot

    Flow cytometry showing C-X-C chemokine receptor type 4 (CXCR-4) surface expression in mesenchymal stem cells (MSCs) labelled with anti-CXCR-4 antibody, from a) young, b) adult control, and c) ovariectomized (OVX) rats. Young MSCs have the highest expression of CXCR-4 (87%), followed by MSCs from adult control (32%); MSCs from OVX rats (19%) had the lowest expression of CXCR-4 (n = 3). In a), the red histogram shows the secondary background control and the green histogram shows the CXCR-4 expression in young MSCs. In figures b) and c), the green histogram demonstrates the background expression and the red histogram shows the CXCR-4 expression. FITC, fluorescein isothiocyanate.

    Journal: Bone & Joint Research

    Article Title: The influence of age and osteoporosis on bone marrow stem cells from rats

    doi: 10.1302/2046-3758.74.BJR-2017-0302.R1

    Figure Lengend Snippet: Flow cytometry showing C-X-C chemokine receptor type 4 (CXCR-4) surface expression in mesenchymal stem cells (MSCs) labelled with anti-CXCR-4 antibody, from a) young, b) adult control, and c) ovariectomized (OVX) rats. Young MSCs have the highest expression of CXCR-4 (87%), followed by MSCs from adult control (32%); MSCs from OVX rats (19%) had the lowest expression of CXCR-4 (n = 3). In a), the red histogram shows the secondary background control and the green histogram shows the CXCR-4 expression in young MSCs. In figures b) and c), the green histogram demonstrates the background expression and the red histogram shows the CXCR-4 expression. FITC, fluorescein isothiocyanate.

    Article Snippet: Cell aliquots were incubated with primary CXCR-4 antibody (Abcam) for 30 minutes at room temperature.

    Techniques: Flow Cytometry, Cytometry, Expressing

    Knockout of Notch3 reduces progenitor cells and worse cardiac function in post-MI. A and B. Western blot analysis of the expression of CXCR-4 and SDF-1α expression revealed that the expression of CXCR4 was significantly reduced in the ischemic

    Journal: International journal of cardiology

    Article Title: Notch3 deficiency impairs coronary microvascular maturation and reduces cardiac recovery after myocardial ischemia

    doi: 10.1016/j.ijcard.2017.01.096

    Figure Lengend Snippet: Knockout of Notch3 reduces progenitor cells and worse cardiac function in post-MI. A and B. Western blot analysis of the expression of CXCR-4 and SDF-1α expression revealed that the expression of CXCR4 was significantly reduced in the ischemic

    Article Snippet: The Polyvinylidene difluoride membranes were probed with antibodies specific to cleaved Notch3, angiopoietin-1 (ANG1), CXCR-4, SDF-1α and vascular endothelial growth factor (VEGF) (Santa Cruz, CA , USA).

    Techniques: Knock-Out, Western Blot, Expressing

    Chemokine receptor expression byB16 melanoma cell lines. Immunoblot analysis results demonstrate that B16-F1, B16α 4 +, and B16α 4 - express similar amounts of the chemokine receptors CCR-10, CCR-7, and CXCR-4. CXCR-3 expression was slightly

    Journal: Neoplasia (New York, N.Y.)

    Article Title: Constitutive Expression of the ?4 Integrin Correlates with Tumorigenicity and Lymph Node Metastasis of the B16 Murine Melanoma 1

    doi:

    Figure Lengend Snippet: Chemokine receptor expression byB16 melanoma cell lines. Immunoblot analysis results demonstrate that B16-F1, B16α 4 +, and B16α 4 - express similar amounts of the chemokine receptors CCR-10, CCR-7, and CXCR-4. CXCR-3 expression was slightly

    Article Snippet: The following antibodies were used in our study: anti-CD49d (integrin α4 ) (553154), anti-CD29 (integrin β1) (553715), anti-vascular endothelial growth factor receptor 3 (VEGFR-3) (552857), anti-CD16/CD32 (553142), anti-VCAM-1 (550547) (BD PharMingen, San Diego, CA); anti-VCAM-1 (sc-31048) and anti-CXCR-3 (sc-133121) from Santa Cruz Biotechnology (Santa Cruz, CA); anti-CXCR-4 (54007; AnaSpec, Fremont, CA); anti-CC-chemokine receptor-7 (CCR-7, 2059-1; Epitomics, Inc, Burlingame, CA); anti-CCR-10 (GTX21661; GeneTex, Inc, Irvine, CA); anti-Prox1 (11-002; AngioBio, Del Mar, CA); anti-β-actin (A5441; Sigma-Aldrich, St. Louis, MO); anti-lymphatic vessel endothelial hyaluronic acid receptor 1 (LYVE-1, 10350PA50S; Fitzgerald Industries, Concord, MA); and anti-VCAM-1 (MCA2297; Serotec, Raleigh, NC).

    Techniques: Expressing

    Northern blot analysis of CXCR-4 and CCR-5 mRNA expression in freshly isolated thymocytes. Three thymocyte preparations from different subjects (lanes 1, 2 and 3) were analysed. The CXCR-4 and CCR-5 messages revealed in a representative freshly isolated PBL sample is also shown. Autoradiographs for CCR-5 were obtained after 5-day exposure.

    Journal: Clinical and Experimental Immunology

    Article Title: Expression and functional activity of CXCR-4 and CCR-5 chemokine receptors in human thymocytes

    doi: 10.1046/j.1365-2249.2002.01775.x

    Figure Lengend Snippet: Northern blot analysis of CXCR-4 and CCR-5 mRNA expression in freshly isolated thymocytes. Three thymocyte preparations from different subjects (lanes 1, 2 and 3) were analysed. The CXCR-4 and CCR-5 messages revealed in a representative freshly isolated PBL sample is also shown. Autoradiographs for CCR-5 were obtained after 5-day exposure.

    Article Snippet: Three-colour immunofluorescence was carried out with the following MoAbs: anti-CD4 ECD (Coulter), anti-CD8 PE (Becton-Dickinson, San Jose, CA, USA), anti-CCR-5 (clone 2D7, PharMingen, San Diego, CA, USA) or anti-CXCR-4 (clone 12G5, PharMingen), in combination with an anti-mouse FITC MoAb (Dako, Glostrup, Denmark).

    Techniques: Northern Blot, Expressing, Isolation

    Percentage expression (a) and surface density (b) of CXCR-4 and CCR-5 co-receptors in different thymocyte subsets. (a) The results represent the relative percentage of CD4 + CD8 – , CD4 + CD8 + and CD4 – CD8 + cells also expressing CXCR-4 (hatched columns) or CCR-5 (open columns). Mean values of six consecutive experiments were reported; the bars indicate the s.d. (b) CXCR-4 (hatched columns), CCR-5 (open columns) and CD4 (closed columns) antibody-binding sites (ABS)/cell were calculated in CD4 + CD8 – , CD4 + CD8 + and CD4 – CD8 + thymocytes. Mean values of six consecutive experiments were reported; the bars indicate the s.d. * P = 0·02 compared to CD4 + CD8 – thymocytes; ** P = 0·02 compared to CD4 + CD8 + thymocytes; *** P

    Journal: Clinical and Experimental Immunology

    Article Title: Expression and functional activity of CXCR-4 and CCR-5 chemokine receptors in human thymocytes

    doi: 10.1046/j.1365-2249.2002.01775.x

    Figure Lengend Snippet: Percentage expression (a) and surface density (b) of CXCR-4 and CCR-5 co-receptors in different thymocyte subsets. (a) The results represent the relative percentage of CD4 + CD8 – , CD4 + CD8 + and CD4 – CD8 + cells also expressing CXCR-4 (hatched columns) or CCR-5 (open columns). Mean values of six consecutive experiments were reported; the bars indicate the s.d. (b) CXCR-4 (hatched columns), CCR-5 (open columns) and CD4 (closed columns) antibody-binding sites (ABS)/cell were calculated in CD4 + CD8 – , CD4 + CD8 + and CD4 – CD8 + thymocytes. Mean values of six consecutive experiments were reported; the bars indicate the s.d. * P = 0·02 compared to CD4 + CD8 – thymocytes; ** P = 0·02 compared to CD4 + CD8 + thymocytes; *** P

    Article Snippet: Three-colour immunofluorescence was carried out with the following MoAbs: anti-CD4 ECD (Coulter), anti-CD8 PE (Becton-Dickinson, San Jose, CA, USA), anti-CCR-5 (clone 2D7, PharMingen, San Diego, CA, USA) or anti-CXCR-4 (clone 12G5, PharMingen), in combination with an anti-mouse FITC MoAb (Dako, Glostrup, Denmark).

    Techniques: Expressing, Binding Assay

    Cytofluorographic analysis of CD3 and CXCR-4/CCR-5 expression in CD4 – CD8 – (double-negative) thymocytes. Unfractionated thymocytes were depleted in CD4 + and CD8 + cells, and the resulting double-negative population was analysed for CD3 and co-receptor expression: (a) anti-CD3 and anti-CXCR-4; (b) anti-CD3 and anti-CCR-5 by two-colour immunofluorescence. One representative experiment of three consecutive experiments is shown; the numbers indicate the percentage of cells included in each section of the plot.

    Journal: Clinical and Experimental Immunology

    Article Title: Expression and functional activity of CXCR-4 and CCR-5 chemokine receptors in human thymocytes

    doi: 10.1046/j.1365-2249.2002.01775.x

    Figure Lengend Snippet: Cytofluorographic analysis of CD3 and CXCR-4/CCR-5 expression in CD4 – CD8 – (double-negative) thymocytes. Unfractionated thymocytes were depleted in CD4 + and CD8 + cells, and the resulting double-negative population was analysed for CD3 and co-receptor expression: (a) anti-CD3 and anti-CXCR-4; (b) anti-CD3 and anti-CCR-5 by two-colour immunofluorescence. One representative experiment of three consecutive experiments is shown; the numbers indicate the percentage of cells included in each section of the plot.

    Article Snippet: Three-colour immunofluorescence was carried out with the following MoAbs: anti-CD4 ECD (Coulter), anti-CD8 PE (Becton-Dickinson, San Jose, CA, USA), anti-CCR-5 (clone 2D7, PharMingen, San Diego, CA, USA) or anti-CXCR-4 (clone 12G5, PharMingen), in combination with an anti-mouse FITC MoAb (Dako, Glostrup, Denmark).

    Techniques: Expressing, Immunofluorescence

    hsa-miR-9 regulates the expression of CXCR-4

    Journal: Journal of thrombosis and haemostasis : JTH

    Article Title: MiR-9 contributes to the Developmental Differences in CXCR-4 Expression in Human Megakaryocytes

    doi: 10.1111/jth.12469

    Figure Lengend Snippet: hsa-miR-9 regulates the expression of CXCR-4

    Article Snippet: CXCR-4 protein levels were determined by Western Blot using a rabbit anti-CXCR-4 (Abcam 2074) and a rabbit anti-beta-actin (Sigma) antibodies.

    Techniques: Expressing

    Differential distribution of Orai1 and TRPM7. A. Confocal images of migrating activated T cells stained for Orai1 (green) together with either CD44 (red) or CXCR-4 (red). Yellow areas in the merge images indicate colocalization. Scale bar = 5 µm. The average correlation coefficients for the uropod (U, n = 21) and the leading-edge (L, n = 22) from 2 donors are shown in the right panel. B. Confocal images of migrating activated T cells that were fixed and stained for TRPM7 (green) together with either CD44 (red) or CXCR-4 (red). Correlation between two proteins is indicated by yellow areas in the merge images. Scale bar = 5 µm. The average correlation coefficients for the uropod (U, n = 29) and the leading-edge (L, n = 12) from 2 donors are shown in the right panel.

    Journal: PLoS ONE

    Article Title: KCa3.1 and TRPM7 Channels at the Uropod Regulate Migration of Activated Human T Cells

    doi: 10.1371/journal.pone.0043859

    Figure Lengend Snippet: Differential distribution of Orai1 and TRPM7. A. Confocal images of migrating activated T cells stained for Orai1 (green) together with either CD44 (red) or CXCR-4 (red). Yellow areas in the merge images indicate colocalization. Scale bar = 5 µm. The average correlation coefficients for the uropod (U, n = 21) and the leading-edge (L, n = 22) from 2 donors are shown in the right panel. B. Confocal images of migrating activated T cells that were fixed and stained for TRPM7 (green) together with either CD44 (red) or CXCR-4 (red). Correlation between two proteins is indicated by yellow areas in the merge images. Scale bar = 5 µm. The average correlation coefficients for the uropod (U, n = 29) and the leading-edge (L, n = 12) from 2 donors are shown in the right panel.

    Article Snippet: The following antibodies were used: mouse anti-CD44 (R & D Systems, #BBA10), mouse anti-CXCR-4 (R & D Systems, #FAB172B), rabbit anti-HA (Sigma Aldrich #H6908), rabbit extracellular anti-Kv1.3 (Millipore, #AB5589), rabbit extracellular anti-Orai1 (Alomone labs, #ACC-060), rabbit intracellular anti-TRPM4 (Aviva Systems Biology, #ARP35268_P050), and goat intracellular anti-TRPM7 (Abcam, #ab729).

    Techniques: Staining

    Differential localization of Kv1.3 and KCa3.1 in migrating T cells. A. Distribution of Kv1.3 and KCa3.1 at the uropod. T cells were transiently transfected with either YFP-KCa3.1 or GFP-Kv1.3 (green) and stained with anti-CD44 antibody (uropod; red) without permeabilization. Yellow areas in the merge images indicate colocalization. Scale bar = 5 µm. B. Distribution of Kv1.3 and KCa3.1 at the leading-edge. T cells, transfected with either YFP-KCa3.1 or GFP-Kv1.3 (green) and stained with anti-CXCR-4 antibody (leading-edge; red) without permeabilization, were analyzed by confocal microscopy. Colocalization between the two proteins is indicated by yellow areas in the merge images. Scale bar = 5 µm. C. Correlation coefficients for KCa3.1 and Kv1.3 localization in the uropod (U) and leading-edge (L). The data are the average of n = 15 cells for KCa3.1 at the U and n = 8 at the L, and n = 16 for Kv1.3 at the U and n = 11 at the U from 2 healthy individuals. Statistical significance was established by one way ANOVA. D. Localization of native Kv1.3 in the leading-edge. T cells from one healthy individual were fixed and stained with extracellular anti-Kv1.3 antibody (green) together with antibodies either against CD44 (red; left) or CXCR-4 (red, right). Yellow colors in the merge images indicate strong correlation. Scale bar = 5 µm. E. Average Correlation coefficients of native Kv1.3 with the leading-edge (L) (n = 9) and the uropod (U) markers (n = 11).

    Journal: PLoS ONE

    Article Title: KCa3.1 and TRPM7 Channels at the Uropod Regulate Migration of Activated Human T Cells

    doi: 10.1371/journal.pone.0043859

    Figure Lengend Snippet: Differential localization of Kv1.3 and KCa3.1 in migrating T cells. A. Distribution of Kv1.3 and KCa3.1 at the uropod. T cells were transiently transfected with either YFP-KCa3.1 or GFP-Kv1.3 (green) and stained with anti-CD44 antibody (uropod; red) without permeabilization. Yellow areas in the merge images indicate colocalization. Scale bar = 5 µm. B. Distribution of Kv1.3 and KCa3.1 at the leading-edge. T cells, transfected with either YFP-KCa3.1 or GFP-Kv1.3 (green) and stained with anti-CXCR-4 antibody (leading-edge; red) without permeabilization, were analyzed by confocal microscopy. Colocalization between the two proteins is indicated by yellow areas in the merge images. Scale bar = 5 µm. C. Correlation coefficients for KCa3.1 and Kv1.3 localization in the uropod (U) and leading-edge (L). The data are the average of n = 15 cells for KCa3.1 at the U and n = 8 at the L, and n = 16 for Kv1.3 at the U and n = 11 at the U from 2 healthy individuals. Statistical significance was established by one way ANOVA. D. Localization of native Kv1.3 in the leading-edge. T cells from one healthy individual were fixed and stained with extracellular anti-Kv1.3 antibody (green) together with antibodies either against CD44 (red; left) or CXCR-4 (red, right). Yellow colors in the merge images indicate strong correlation. Scale bar = 5 µm. E. Average Correlation coefficients of native Kv1.3 with the leading-edge (L) (n = 9) and the uropod (U) markers (n = 11).

    Article Snippet: The following antibodies were used: mouse anti-CD44 (R & D Systems, #BBA10), mouse anti-CXCR-4 (R & D Systems, #FAB172B), rabbit anti-HA (Sigma Aldrich #H6908), rabbit extracellular anti-Kv1.3 (Millipore, #AB5589), rabbit extracellular anti-Orai1 (Alomone labs, #ACC-060), rabbit intracellular anti-TRPM4 (Aviva Systems Biology, #ARP35268_P050), and goat intracellular anti-TRPM7 (Abcam, #ab729).

    Techniques: Transfection, Staining, Confocal Microscopy

    EPO administration after myocardial infarction increased homing of EGFP + BMCs into ischaemic myocardium in GFP-transgenic mice. (A) Bar graph representing the percentage of myocardial EGFP + cell populations (subclassified by CD31, c-kit, CXCR-4, Sca-1) of infarcted control mice (white bar) or EPO-treated mice (black bar). All data represent mean ± S.E.M. ( n = 8). (B) Bar graph representing the fold-increase of EGFP + subpopulations in the hearts of infarcted control mice (white bar) or EPO-treated mice (black bar). The represented value is the ratio of the mean of control mice and the mean of EPO-treated mice.

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Migration of bone marrow-derived cells and improved perfusion after treatment with erythropoietin in a murine model of myocardial infarction

    doi: 10.1111/j.1582-4934.2011.01286.x

    Figure Lengend Snippet: EPO administration after myocardial infarction increased homing of EGFP + BMCs into ischaemic myocardium in GFP-transgenic mice. (A) Bar graph representing the percentage of myocardial EGFP + cell populations (subclassified by CD31, c-kit, CXCR-4, Sca-1) of infarcted control mice (white bar) or EPO-treated mice (black bar). All data represent mean ± S.E.M. ( n = 8). (B) Bar graph representing the fold-increase of EGFP + subpopulations in the hearts of infarcted control mice (white bar) or EPO-treated mice (black bar). The represented value is the ratio of the mean of control mice and the mean of EPO-treated mice.

    Article Snippet: Cells from BM, PB and heart were incubated for 40 minutes in the dark at 4°C with the phycoerythrin (PE) conjugated monoclonal antibodies: CD31-PE, c-kit-PE, CXCR-4-PE, Sca-1-PE (all from BD Pharmingen, Heidelberg, Germany).

    Techniques: Transgenic Assay, Mouse Assay

    EPO administration after myocardial infarction increased mobilization of EGFP + BMCs from BM into peripheral blood in GFP-transgenic mice. (A) Representative FACS analyses of EGFP + cells in bone marrow (left) and peripheral blood (right) of wild-type mice 10 weeks after BM-transplantation showing successful BM replacement. (B) Bar graph representing the percentage of EGFP + cell populations (subclassified by CD31, c-kit, CXCR-4, Sca-1) in peripheral blood of infarcted control mice (white bar) or EPO-treated mice (black bar). All data represent mean ± S.E.M. ( n = 8).

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Migration of bone marrow-derived cells and improved perfusion after treatment with erythropoietin in a murine model of myocardial infarction

    doi: 10.1111/j.1582-4934.2011.01286.x

    Figure Lengend Snippet: EPO administration after myocardial infarction increased mobilization of EGFP + BMCs from BM into peripheral blood in GFP-transgenic mice. (A) Representative FACS analyses of EGFP + cells in bone marrow (left) and peripheral blood (right) of wild-type mice 10 weeks after BM-transplantation showing successful BM replacement. (B) Bar graph representing the percentage of EGFP + cell populations (subclassified by CD31, c-kit, CXCR-4, Sca-1) in peripheral blood of infarcted control mice (white bar) or EPO-treated mice (black bar). All data represent mean ± S.E.M. ( n = 8).

    Article Snippet: Cells from BM, PB and heart were incubated for 40 minutes in the dark at 4°C with the phycoerythrin (PE) conjugated monoclonal antibodies: CD31-PE, c-kit-PE, CXCR-4-PE, Sca-1-PE (all from BD Pharmingen, Heidelberg, Germany).

    Techniques: Transgenic Assay, Mouse Assay, FACS, Transplantation Assay