Article Title: CXCR2 and CXCL4 regulate survival and self-renewal of hematopoietic stem/progenitor cells
Figure Lengend Snippet: Inhibition of Cxcl4 reduces colony formation in vitro and Cxcl4 −/− HSCs show a reduction in engraftment in secondary BM transplantation assays. (A) WT BM cells enriched for c-Kit + were transduced with a Cxcl4 -shRNA vector or control, and
Article Snippet: Cells were fixed and permeabilized with 4% formaldehyde and 0.25% Triton X-100, blocked in 10% goat or donkey serum (Sigma-Aldrich) and incubated overnight in primary antibodies against CXCL1 (1:100, sc-1374; Santa Cruz, Heidelberg, Germany), CXCL2 (1:100, sc-1375; Santa Cruz), CXCL4 (1:100, sc-73638; Santa Cruz), CXCL6 (1:100, sc-5813; Santa Cruz), CXCL8 (1:100, MAB208; R & D Systems, Abingdon, United Kingdom), CXCR2 (1:100, sc-682; Santa Cruz), or immunoglobulin G (AB-108-C; R & D Systems).
Techniques: Inhibition, In Vitro, Transplantation Assay, Transduction, shRNA, Plasmid Preparation