cut-off molecular weight Search Results


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  • 99
    Millipore molecular weight cutoff
    Characterisation of the ultracentrifugal filters and the HA ELISA-like assay used for determining sHA concentrations in TIF. ( A ) A digest of <t>HMW-HA</t> containing a mixture of HA fragments was prepared (HA digest) and centrifuged through a 10 kDa <t>MWCO</t> ultracentrifugal filter (10 kDa). Fluorophore-assisted Carbohydrate electrophoresis analysis was used to detect the HA fragments before and after centrifugation. Purified HA fragments of defined size (13–15 disaccharides) were used as reference standards. The numbers on the right of the figure indicate the length of the indicated HA oligosaccharides. Ds: disaccharides. ( B ) A digest of HMW-HA containing a mixture of HA fragments was prepared and centrifuged through a 10-kDa MWCO ultracentrifugal filter (sHA mix). The HA ELISA-like assay was used to quantify the concentration of the sHA mix in comparison to an equivalent amount of undigested HMW-HA. ( C ) Hyaluronic acid fragments of defined size were purified from a HA digest using size-exclusion chromatography. Equivalent molarities (250 n M ) of HA fragments of defined length were analysed using the HA ELISA-like assay. The apparent concentration of the samples as measured in the ELISA-like assay is presented. The size of the HA oligosaccharides measured is indicated in disaccharides. Buffer served as a control.
    Molecular Weight Cutoff, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 726 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore molecular weight cut off
    Characterisation of the ultracentrifugal filters and the HA ELISA-like assay used for determining sHA concentrations in TIF. ( A ) A digest of <t>HMW-HA</t> containing a mixture of HA fragments was prepared (HA digest) and centrifuged through a 10 kDa <t>MWCO</t> ultracentrifugal filter (10 kDa). Fluorophore-assisted Carbohydrate electrophoresis analysis was used to detect the HA fragments before and after centrifugation. Purified HA fragments of defined size (13–15 disaccharides) were used as reference standards. The numbers on the right of the figure indicate the length of the indicated HA oligosaccharides. Ds: disaccharides. ( B ) A digest of HMW-HA containing a mixture of HA fragments was prepared and centrifuged through a 10-kDa MWCO ultracentrifugal filter (sHA mix). The HA ELISA-like assay was used to quantify the concentration of the sHA mix in comparison to an equivalent amount of undigested HMW-HA. ( C ) Hyaluronic acid fragments of defined size were purified from a HA digest using size-exclusion chromatography. Equivalent molarities (250 n M ) of HA fragments of defined length were analysed using the HA ELISA-like assay. The apparent concentration of the samples as measured in the ELISA-like assay is presented. The size of the HA oligosaccharides measured is indicated in disaccharides. Buffer served as a control.
    Molecular Weight Cut Off, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1045 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher molecular weight cut off
    Characterisation of the ultracentrifugal filters and the HA ELISA-like assay used for determining sHA concentrations in TIF. ( A ) A digest of <t>HMW-HA</t> containing a mixture of HA fragments was prepared (HA digest) and centrifuged through a 10 kDa <t>MWCO</t> ultracentrifugal filter (10 kDa). Fluorophore-assisted Carbohydrate electrophoresis analysis was used to detect the HA fragments before and after centrifugation. Purified HA fragments of defined size (13–15 disaccharides) were used as reference standards. The numbers on the right of the figure indicate the length of the indicated HA oligosaccharides. Ds: disaccharides. ( B ) A digest of HMW-HA containing a mixture of HA fragments was prepared and centrifuged through a 10-kDa MWCO ultracentrifugal filter (sHA mix). The HA ELISA-like assay was used to quantify the concentration of the sHA mix in comparison to an equivalent amount of undigested HMW-HA. ( C ) Hyaluronic acid fragments of defined size were purified from a HA digest using size-exclusion chromatography. Equivalent molarities (250 n M ) of HA fragments of defined length were analysed using the HA ELISA-like assay. The apparent concentration of the samples as measured in the ELISA-like assay is presented. The size of the HA oligosaccharides measured is indicated in disaccharides. Buffer served as a control.
    Molecular Weight Cut Off, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 267 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Merck KGaA molecular weight cut off
    Characterisation of the ultracentrifugal filters and the HA ELISA-like assay used for determining sHA concentrations in TIF. ( A ) A digest of <t>HMW-HA</t> containing a mixture of HA fragments was prepared (HA digest) and centrifuged through a 10 kDa <t>MWCO</t> ultracentrifugal filter (10 kDa). Fluorophore-assisted Carbohydrate electrophoresis analysis was used to detect the HA fragments before and after centrifugation. Purified HA fragments of defined size (13–15 disaccharides) were used as reference standards. The numbers on the right of the figure indicate the length of the indicated HA oligosaccharides. Ds: disaccharides. ( B ) A digest of HMW-HA containing a mixture of HA fragments was prepared and centrifuged through a 10-kDa MWCO ultracentrifugal filter (sHA mix). The HA ELISA-like assay was used to quantify the concentration of the sHA mix in comparison to an equivalent amount of undigested HMW-HA. ( C ) Hyaluronic acid fragments of defined size were purified from a HA digest using size-exclusion chromatography. Equivalent molarities (250 n M ) of HA fragments of defined length were analysed using the HA ELISA-like assay. The apparent concentration of the samples as measured in the ELISA-like assay is presented. The size of the HA oligosaccharides measured is indicated in disaccharides. Buffer served as a control.
    Molecular Weight Cut Off, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 188 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Spectrum Labs molecular weight cut off
    Characterisation of the ultracentrifugal filters and the HA ELISA-like assay used for determining sHA concentrations in TIF. ( A ) A digest of <t>HMW-HA</t> containing a mixture of HA fragments was prepared (HA digest) and centrifuged through a 10 kDa <t>MWCO</t> ultracentrifugal filter (10 kDa). Fluorophore-assisted Carbohydrate electrophoresis analysis was used to detect the HA fragments before and after centrifugation. Purified HA fragments of defined size (13–15 disaccharides) were used as reference standards. The numbers on the right of the figure indicate the length of the indicated HA oligosaccharides. Ds: disaccharides. ( B ) A digest of HMW-HA containing a mixture of HA fragments was prepared and centrifuged through a 10-kDa MWCO ultracentrifugal filter (sHA mix). The HA ELISA-like assay was used to quantify the concentration of the sHA mix in comparison to an equivalent amount of undigested HMW-HA. ( C ) Hyaluronic acid fragments of defined size were purified from a HA digest using size-exclusion chromatography. Equivalent molarities (250 n M ) of HA fragments of defined length were analysed using the HA ELISA-like assay. The apparent concentration of the samples as measured in the ELISA-like assay is presented. The size of the HA oligosaccharides measured is indicated in disaccharides. Buffer served as a control.
    Molecular Weight Cut Off, supplied by Spectrum Labs, used in various techniques. Bioz Stars score: 92/100, based on 147 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Sartorius AG molecular weight cut off
    Characterisation of the ultracentrifugal filters and the HA ELISA-like assay used for determining sHA concentrations in TIF. ( A ) A digest of <t>HMW-HA</t> containing a mixture of HA fragments was prepared (HA digest) and centrifuged through a 10 kDa <t>MWCO</t> ultracentrifugal filter (10 kDa). Fluorophore-assisted Carbohydrate electrophoresis analysis was used to detect the HA fragments before and after centrifugation. Purified HA fragments of defined size (13–15 disaccharides) were used as reference standards. The numbers on the right of the figure indicate the length of the indicated HA oligosaccharides. Ds: disaccharides. ( B ) A digest of HMW-HA containing a mixture of HA fragments was prepared and centrifuged through a 10-kDa MWCO ultracentrifugal filter (sHA mix). The HA ELISA-like assay was used to quantify the concentration of the sHA mix in comparison to an equivalent amount of undigested HMW-HA. ( C ) Hyaluronic acid fragments of defined size were purified from a HA digest using size-exclusion chromatography. Equivalent molarities (250 n M ) of HA fragments of defined length were analysed using the HA ELISA-like assay. The apparent concentration of the samples as measured in the ELISA-like assay is presented. The size of the HA oligosaccharides measured is indicated in disaccharides. Buffer served as a control.
    Molecular Weight Cut Off, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 92/100, based on 180 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    GE Healthcare molecular weight cut off
    Characterisation of the ultracentrifugal filters and the HA ELISA-like assay used for determining sHA concentrations in TIF. ( A ) A digest of <t>HMW-HA</t> containing a mixture of HA fragments was prepared (HA digest) and centrifuged through a 10 kDa <t>MWCO</t> ultracentrifugal filter (10 kDa). Fluorophore-assisted Carbohydrate electrophoresis analysis was used to detect the HA fragments before and after centrifugation. Purified HA fragments of defined size (13–15 disaccharides) were used as reference standards. The numbers on the right of the figure indicate the length of the indicated HA oligosaccharides. Ds: disaccharides. ( B ) A digest of HMW-HA containing a mixture of HA fragments was prepared and centrifuged through a 10-kDa MWCO ultracentrifugal filter (sHA mix). The HA ELISA-like assay was used to quantify the concentration of the sHA mix in comparison to an equivalent amount of undigested HMW-HA. ( C ) Hyaluronic acid fragments of defined size were purified from a HA digest using size-exclusion chromatography. Equivalent molarities (250 n M ) of HA fragments of defined length were analysed using the HA ELISA-like assay. The apparent concentration of the samples as measured in the ELISA-like assay is presented. The size of the HA oligosaccharides measured is indicated in disaccharides. Buffer served as a control.
    Molecular Weight Cut Off, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 95/100, based on 146 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher molecular weight cutoff mwco
    Characterisation of the ultracentrifugal filters and the HA ELISA-like assay used for determining sHA concentrations in TIF. ( A ) A digest of <t>HMW-HA</t> containing a mixture of HA fragments was prepared (HA digest) and centrifuged through a 10 kDa <t>MWCO</t> ultracentrifugal filter (10 kDa). Fluorophore-assisted Carbohydrate electrophoresis analysis was used to detect the HA fragments before and after centrifugation. Purified HA fragments of defined size (13–15 disaccharides) were used as reference standards. The numbers on the right of the figure indicate the length of the indicated HA oligosaccharides. Ds: disaccharides. ( B ) A digest of HMW-HA containing a mixture of HA fragments was prepared and centrifuged through a 10-kDa MWCO ultracentrifugal filter (sHA mix). The HA ELISA-like assay was used to quantify the concentration of the sHA mix in comparison to an equivalent amount of undigested HMW-HA. ( C ) Hyaluronic acid fragments of defined size were purified from a HA digest using size-exclusion chromatography. Equivalent molarities (250 n M ) of HA fragments of defined length were analysed using the HA ELISA-like assay. The apparent concentration of the samples as measured in the ELISA-like assay is presented. The size of the HA oligosaccharides measured is indicated in disaccharides. Buffer served as a control.
    Molecular Weight Cutoff Mwco, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 76 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore 10 kda molecular weight cutoff
    Characterisation of the ultracentrifugal filters and the HA ELISA-like assay used for determining sHA concentrations in TIF. ( A ) A digest of <t>HMW-HA</t> containing a mixture of HA fragments was prepared (HA digest) and centrifuged through a 10 kDa <t>MWCO</t> ultracentrifugal filter (10 kDa). Fluorophore-assisted Carbohydrate electrophoresis analysis was used to detect the HA fragments before and after centrifugation. Purified HA fragments of defined size (13–15 disaccharides) were used as reference standards. The numbers on the right of the figure indicate the length of the indicated HA oligosaccharides. Ds: disaccharides. ( B ) A digest of HMW-HA containing a mixture of HA fragments was prepared and centrifuged through a 10-kDa MWCO ultracentrifugal filter (sHA mix). The HA ELISA-like assay was used to quantify the concentration of the sHA mix in comparison to an equivalent amount of undigested HMW-HA. ( C ) Hyaluronic acid fragments of defined size were purified from a HA digest using size-exclusion chromatography. Equivalent molarities (250 n M ) of HA fragments of defined length were analysed using the HA ELISA-like assay. The apparent concentration of the samples as measured in the ELISA-like assay is presented. The size of the HA oligosaccharides measured is indicated in disaccharides. Buffer served as a control.
    10 Kda Molecular Weight Cutoff, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 105 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Spectrum Labs molecular weight cutoff mwco
    Characterisation of the ultracentrifugal filters and the HA ELISA-like assay used for determining sHA concentrations in TIF. ( A ) A digest of <t>HMW-HA</t> containing a mixture of HA fragments was prepared (HA digest) and centrifuged through a 10 kDa <t>MWCO</t> ultracentrifugal filter (10 kDa). Fluorophore-assisted Carbohydrate electrophoresis analysis was used to detect the HA fragments before and after centrifugation. Purified HA fragments of defined size (13–15 disaccharides) were used as reference standards. The numbers on the right of the figure indicate the length of the indicated HA oligosaccharides. Ds: disaccharides. ( B ) A digest of HMW-HA containing a mixture of HA fragments was prepared and centrifuged through a 10-kDa MWCO ultracentrifugal filter (sHA mix). The HA ELISA-like assay was used to quantify the concentration of the sHA mix in comparison to an equivalent amount of undigested HMW-HA. ( C ) Hyaluronic acid fragments of defined size were purified from a HA digest using size-exclusion chromatography. Equivalent molarities (250 n M ) of HA fragments of defined length were analysed using the HA ELISA-like assay. The apparent concentration of the samples as measured in the ELISA-like assay is presented. The size of the HA oligosaccharides measured is indicated in disaccharides. Buffer served as a control.
    Molecular Weight Cutoff Mwco, supplied by Spectrum Labs, used in various techniques. Bioz Stars score: 91/100, based on 108 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Thermo Fisher molecular weight cut off dialysis cassettes
    Characterisation of the ultracentrifugal filters and the HA ELISA-like assay used for determining sHA concentrations in TIF. ( A ) A digest of <t>HMW-HA</t> containing a mixture of HA fragments was prepared (HA digest) and centrifuged through a 10 kDa <t>MWCO</t> ultracentrifugal filter (10 kDa). Fluorophore-assisted Carbohydrate electrophoresis analysis was used to detect the HA fragments before and after centrifugation. Purified HA fragments of defined size (13–15 disaccharides) were used as reference standards. The numbers on the right of the figure indicate the length of the indicated HA oligosaccharides. Ds: disaccharides. ( B ) A digest of HMW-HA containing a mixture of HA fragments was prepared and centrifuged through a 10-kDa MWCO ultracentrifugal filter (sHA mix). The HA ELISA-like assay was used to quantify the concentration of the sHA mix in comparison to an equivalent amount of undigested HMW-HA. ( C ) Hyaluronic acid fragments of defined size were purified from a HA digest using size-exclusion chromatography. Equivalent molarities (250 n M ) of HA fragments of defined length were analysed using the HA ELISA-like assay. The apparent concentration of the samples as measured in the ELISA-like assay is presented. The size of the HA oligosaccharides measured is indicated in disaccharides. Buffer served as a control.
    Molecular Weight Cut Off Dialysis Cassettes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Spectrum Labs molecular weight cutoff dialysis tubing
    Characterisation of the ultracentrifugal filters and the HA ELISA-like assay used for determining sHA concentrations in TIF. ( A ) A digest of <t>HMW-HA</t> containing a mixture of HA fragments was prepared (HA digest) and centrifuged through a 10 kDa <t>MWCO</t> ultracentrifugal filter (10 kDa). Fluorophore-assisted Carbohydrate electrophoresis analysis was used to detect the HA fragments before and after centrifugation. Purified HA fragments of defined size (13–15 disaccharides) were used as reference standards. The numbers on the right of the figure indicate the length of the indicated HA oligosaccharides. Ds: disaccharides. ( B ) A digest of HMW-HA containing a mixture of HA fragments was prepared and centrifuged through a 10-kDa MWCO ultracentrifugal filter (sHA mix). The HA ELISA-like assay was used to quantify the concentration of the sHA mix in comparison to an equivalent amount of undigested HMW-HA. ( C ) Hyaluronic acid fragments of defined size were purified from a HA digest using size-exclusion chromatography. Equivalent molarities (250 n M ) of HA fragments of defined length were analysed using the HA ELISA-like assay. The apparent concentration of the samples as measured in the ELISA-like assay is presented. The size of the HA oligosaccharides measured is indicated in disaccharides. Buffer served as a control.
    Molecular Weight Cutoff Dialysis Tubing, supplied by Spectrum Labs, used in various techniques. Bioz Stars score: 89/100, based on 104 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Sartorius AG molecular weight cutoff filter
    Characterisation of the ultracentrifugal filters and the HA ELISA-like assay used for determining sHA concentrations in TIF. ( A ) A digest of <t>HMW-HA</t> containing a mixture of HA fragments was prepared (HA digest) and centrifuged through a 10 kDa <t>MWCO</t> ultracentrifugal filter (10 kDa). Fluorophore-assisted Carbohydrate electrophoresis analysis was used to detect the HA fragments before and after centrifugation. Purified HA fragments of defined size (13–15 disaccharides) were used as reference standards. The numbers on the right of the figure indicate the length of the indicated HA oligosaccharides. Ds: disaccharides. ( B ) A digest of HMW-HA containing a mixture of HA fragments was prepared and centrifuged through a 10-kDa MWCO ultracentrifugal filter (sHA mix). The HA ELISA-like assay was used to quantify the concentration of the sHA mix in comparison to an equivalent amount of undigested HMW-HA. ( C ) Hyaluronic acid fragments of defined size were purified from a HA digest using size-exclusion chromatography. Equivalent molarities (250 n M ) of HA fragments of defined length were analysed using the HA ELISA-like assay. The apparent concentration of the samples as measured in the ELISA-like assay is presented. The size of the HA oligosaccharides measured is indicated in disaccharides. Buffer served as a control.
    Molecular Weight Cutoff Filter, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 90/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Thermo Fisher 10 kda molecular weight cut off
    Characterisation of the ultracentrifugal filters and the HA ELISA-like assay used for determining sHA concentrations in TIF. ( A ) A digest of <t>HMW-HA</t> containing a mixture of HA fragments was prepared (HA digest) and centrifuged through a 10 kDa <t>MWCO</t> ultracentrifugal filter (10 kDa). Fluorophore-assisted Carbohydrate electrophoresis analysis was used to detect the HA fragments before and after centrifugation. Purified HA fragments of defined size (13–15 disaccharides) were used as reference standards. The numbers on the right of the figure indicate the length of the indicated HA oligosaccharides. Ds: disaccharides. ( B ) A digest of HMW-HA containing a mixture of HA fragments was prepared and centrifuged through a 10-kDa MWCO ultracentrifugal filter (sHA mix). The HA ELISA-like assay was used to quantify the concentration of the sHA mix in comparison to an equivalent amount of undigested HMW-HA. ( C ) Hyaluronic acid fragments of defined size were purified from a HA digest using size-exclusion chromatography. Equivalent molarities (250 n M ) of HA fragments of defined length were analysed using the HA ELISA-like assay. The apparent concentration of the samples as measured in the ELISA-like assay is presented. The size of the HA oligosaccharides measured is indicated in disaccharides. Buffer served as a control.
    10 Kda Molecular Weight Cut Off, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore molecular weight cutoff membrane
    Characterisation of the ultracentrifugal filters and the HA ELISA-like assay used for determining sHA concentrations in TIF. ( A ) A digest of <t>HMW-HA</t> containing a mixture of HA fragments was prepared (HA digest) and centrifuged through a 10 kDa <t>MWCO</t> ultracentrifugal filter (10 kDa). Fluorophore-assisted Carbohydrate electrophoresis analysis was used to detect the HA fragments before and after centrifugation. Purified HA fragments of defined size (13–15 disaccharides) were used as reference standards. The numbers on the right of the figure indicate the length of the indicated HA oligosaccharides. Ds: disaccharides. ( B ) A digest of HMW-HA containing a mixture of HA fragments was prepared and centrifuged through a 10-kDa MWCO ultracentrifugal filter (sHA mix). The HA ELISA-like assay was used to quantify the concentration of the sHA mix in comparison to an equivalent amount of undigested HMW-HA. ( C ) Hyaluronic acid fragments of defined size were purified from a HA digest using size-exclusion chromatography. Equivalent molarities (250 n M ) of HA fragments of defined length were analysed using the HA ELISA-like assay. The apparent concentration of the samples as measured in the ELISA-like assay is presented. The size of the HA oligosaccharides measured is indicated in disaccharides. Buffer served as a control.
    Molecular Weight Cutoff Membrane, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 215 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Spectrum Labs molecular weight cutoff membrane
    Characterisation of the ultracentrifugal filters and the HA ELISA-like assay used for determining sHA concentrations in TIF. ( A ) A digest of <t>HMW-HA</t> containing a mixture of HA fragments was prepared (HA digest) and centrifuged through a 10 kDa <t>MWCO</t> ultracentrifugal filter (10 kDa). Fluorophore-assisted Carbohydrate electrophoresis analysis was used to detect the HA fragments before and after centrifugation. Purified HA fragments of defined size (13–15 disaccharides) were used as reference standards. The numbers on the right of the figure indicate the length of the indicated HA oligosaccharides. Ds: disaccharides. ( B ) A digest of HMW-HA containing a mixture of HA fragments was prepared and centrifuged through a 10-kDa MWCO ultracentrifugal filter (sHA mix). The HA ELISA-like assay was used to quantify the concentration of the sHA mix in comparison to an equivalent amount of undigested HMW-HA. ( C ) Hyaluronic acid fragments of defined size were purified from a HA digest using size-exclusion chromatography. Equivalent molarities (250 n M ) of HA fragments of defined length were analysed using the HA ELISA-like assay. The apparent concentration of the samples as measured in the ELISA-like assay is presented. The size of the HA oligosaccharides measured is indicated in disaccharides. Buffer served as a control.
    Molecular Weight Cutoff Membrane, supplied by Spectrum Labs, used in various techniques. Bioz Stars score: 93/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore molecular weight cutoff filter
    Characterisation of the ultracentrifugal filters and the HA ELISA-like assay used for determining sHA concentrations in TIF. ( A ) A digest of <t>HMW-HA</t> containing a mixture of HA fragments was prepared (HA digest) and centrifuged through a 10 kDa <t>MWCO</t> ultracentrifugal filter (10 kDa). Fluorophore-assisted Carbohydrate electrophoresis analysis was used to detect the HA fragments before and after centrifugation. Purified HA fragments of defined size (13–15 disaccharides) were used as reference standards. The numbers on the right of the figure indicate the length of the indicated HA oligosaccharides. Ds: disaccharides. ( B ) A digest of HMW-HA containing a mixture of HA fragments was prepared and centrifuged through a 10-kDa MWCO ultracentrifugal filter (sHA mix). The HA ELISA-like assay was used to quantify the concentration of the sHA mix in comparison to an equivalent amount of undigested HMW-HA. ( C ) Hyaluronic acid fragments of defined size were purified from a HA digest using size-exclusion chromatography. Equivalent molarities (250 n M ) of HA fragments of defined length were analysed using the HA ELISA-like assay. The apparent concentration of the samples as measured in the ELISA-like assay is presented. The size of the HA oligosaccharides measured is indicated in disaccharides. Buffer served as a control.
    Molecular Weight Cutoff Filter, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 71 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Sartorius AG molecular weight cutoff mwco
    Characterisation of the ultracentrifugal filters and the HA ELISA-like assay used for determining sHA concentrations in TIF. ( A ) A digest of <t>HMW-HA</t> containing a mixture of HA fragments was prepared (HA digest) and centrifuged through a 10 kDa <t>MWCO</t> ultracentrifugal filter (10 kDa). Fluorophore-assisted Carbohydrate electrophoresis analysis was used to detect the HA fragments before and after centrifugation. Purified HA fragments of defined size (13–15 disaccharides) were used as reference standards. The numbers on the right of the figure indicate the length of the indicated HA oligosaccharides. Ds: disaccharides. ( B ) A digest of HMW-HA containing a mixture of HA fragments was prepared and centrifuged through a 10-kDa MWCO ultracentrifugal filter (sHA mix). The HA ELISA-like assay was used to quantify the concentration of the sHA mix in comparison to an equivalent amount of undigested HMW-HA. ( C ) Hyaluronic acid fragments of defined size were purified from a HA digest using size-exclusion chromatography. Equivalent molarities (250 n M ) of HA fragments of defined length were analysed using the HA ELISA-like assay. The apparent concentration of the samples as measured in the ELISA-like assay is presented. The size of the HA oligosaccharides measured is indicated in disaccharides. Buffer served as a control.
    Molecular Weight Cutoff Mwco, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 90/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Characterisation of the ultracentrifugal filters and the HA ELISA-like assay used for determining sHA concentrations in TIF. ( A ) A digest of HMW-HA containing a mixture of HA fragments was prepared (HA digest) and centrifuged through a 10 kDa MWCO ultracentrifugal filter (10 kDa). Fluorophore-assisted Carbohydrate electrophoresis analysis was used to detect the HA fragments before and after centrifugation. Purified HA fragments of defined size (13–15 disaccharides) were used as reference standards. The numbers on the right of the figure indicate the length of the indicated HA oligosaccharides. Ds: disaccharides. ( B ) A digest of HMW-HA containing a mixture of HA fragments was prepared and centrifuged through a 10-kDa MWCO ultracentrifugal filter (sHA mix). The HA ELISA-like assay was used to quantify the concentration of the sHA mix in comparison to an equivalent amount of undigested HMW-HA. ( C ) Hyaluronic acid fragments of defined size were purified from a HA digest using size-exclusion chromatography. Equivalent molarities (250 n M ) of HA fragments of defined length were analysed using the HA ELISA-like assay. The apparent concentration of the samples as measured in the ELISA-like assay is presented. The size of the HA oligosaccharides measured is indicated in disaccharides. Buffer served as a control.

    Journal: British Journal of Cancer

    Article Title: Accumulation of small hyaluronan oligosaccharides in tumour interstitial fluid correlates with lymphatic invasion and lymph node metastasis

    doi: 10.1038/bjc.2014.332

    Figure Lengend Snippet: Characterisation of the ultracentrifugal filters and the HA ELISA-like assay used for determining sHA concentrations in TIF. ( A ) A digest of HMW-HA containing a mixture of HA fragments was prepared (HA digest) and centrifuged through a 10 kDa MWCO ultracentrifugal filter (10 kDa). Fluorophore-assisted Carbohydrate electrophoresis analysis was used to detect the HA fragments before and after centrifugation. Purified HA fragments of defined size (13–15 disaccharides) were used as reference standards. The numbers on the right of the figure indicate the length of the indicated HA oligosaccharides. Ds: disaccharides. ( B ) A digest of HMW-HA containing a mixture of HA fragments was prepared and centrifuged through a 10-kDa MWCO ultracentrifugal filter (sHA mix). The HA ELISA-like assay was used to quantify the concentration of the sHA mix in comparison to an equivalent amount of undigested HMW-HA. ( C ) Hyaluronic acid fragments of defined size were purified from a HA digest using size-exclusion chromatography. Equivalent molarities (250 n M ) of HA fragments of defined length were analysed using the HA ELISA-like assay. The apparent concentration of the samples as measured in the ELISA-like assay is presented. The size of the HA oligosaccharides measured is indicated in disaccharides. Buffer served as a control.

    Article Snippet: To separate sHA from HMW-HA, interstitial fluid was centrifuged through Amicon Ultracentrifugal Filters with a molecular weight cutoff (MWCO) of 10 kDa (Millipore, Billerica, MA, USA).

    Techniques: Enzyme-linked Immunosorbent Assay, Electrophoresis, Centrifugation, Purification, Concentration Assay, Size-exclusion Chromatography

    Assessment of total HA and sHA levels in TIF from syngeneic rat tumours and normal tissues. ( A , B ) TIF was isolated by low-speed centrifugation from the indicated freshly excised rat tumours. For separation of sHA from HMW-HA, TIF was centrifuged through 10-kDa MWCO ultracentrifugal filters. Using the HA ELISA-like assay the concentration of total HA was measured in the TIFs, and the concentration of sHA was measured in the ultracentrifugation filtrates. ( A ) The mean of total HA and sHA concentrations for three independent tumours for each of the indicated tumour models is shown together with the SE. ( B ) sHA concentrations in a panel of rat tumour models. Between 7 and 10 independent tumour-bearing animals were analysed for each tumour model. Each data point represents the sHA concentration in the TIF from one tumour. Bars represent the mean of all data from one group. ( C ) Assessment of physiological total HA and sHA levels in interstitial fluid of rat organs and in rat sera. Interstitial fluid was isolated from the indicated freshly excised rat organs. To separate sHA from HMW-HA, the interstitial fluid or sera were centrifuged through 10-kDa MWCO ultracentrifugal filters. The concentration of total HA in the interstitial fluid or sera, as well as the concentration of sHA in the ultracentrifugation filtrates was measured using the HA ELISA-like assay. The mean of total HA and sHA concentrations for six independent samples for each organ or sera is shown together with the SE.

    Journal: British Journal of Cancer

    Article Title: Accumulation of small hyaluronan oligosaccharides in tumour interstitial fluid correlates with lymphatic invasion and lymph node metastasis

    doi: 10.1038/bjc.2014.332

    Figure Lengend Snippet: Assessment of total HA and sHA levels in TIF from syngeneic rat tumours and normal tissues. ( A , B ) TIF was isolated by low-speed centrifugation from the indicated freshly excised rat tumours. For separation of sHA from HMW-HA, TIF was centrifuged through 10-kDa MWCO ultracentrifugal filters. Using the HA ELISA-like assay the concentration of total HA was measured in the TIFs, and the concentration of sHA was measured in the ultracentrifugation filtrates. ( A ) The mean of total HA and sHA concentrations for three independent tumours for each of the indicated tumour models is shown together with the SE. ( B ) sHA concentrations in a panel of rat tumour models. Between 7 and 10 independent tumour-bearing animals were analysed for each tumour model. Each data point represents the sHA concentration in the TIF from one tumour. Bars represent the mean of all data from one group. ( C ) Assessment of physiological total HA and sHA levels in interstitial fluid of rat organs and in rat sera. Interstitial fluid was isolated from the indicated freshly excised rat organs. To separate sHA from HMW-HA, the interstitial fluid or sera were centrifuged through 10-kDa MWCO ultracentrifugal filters. The concentration of total HA in the interstitial fluid or sera, as well as the concentration of sHA in the ultracentrifugation filtrates was measured using the HA ELISA-like assay. The mean of total HA and sHA concentrations for six independent samples for each organ or sera is shown together with the SE.

    Article Snippet: To separate sHA from HMW-HA, interstitial fluid was centrifuged through Amicon Ultracentrifugal Filters with a molecular weight cutoff (MWCO) of 10 kDa (Millipore, Billerica, MA, USA).

    Techniques: Isolation, Centrifugation, Enzyme-linked Immunosorbent Assay, Concentration Assay