Journal: Journal of Nanobiotechnology

Article Title: TROP2-directed nanobody-drug conjugate elicited potent antitumor effect in pancreatic cancer

doi: 10.1186/s12951-023-02183-9

Figure Lengend Snippet: Endocytosis and in vitro antitumor activity of HuNb TROP2-HSA -MMAE. A The process of HuNb TROP2-HSA -MMAE internalization into BxPC-3 cells. Blue: nuclei stained by Hoechst33342; green: HuNb TROP2-HSA -MMAE labeled with Alexa Fluor™ 488. B HuNb TROP2-HSA -MMAE underwent lysosomal degradation after internalization. Blue: nuclei; green: HuNb TROP2-HSA -MMAE; red: lysosomes. C The expression of TROP2 on the surface of three human pancreatic cell lines was determined by flow cytometry and the cytotoxic effects of HuNb TROP2-HSA -MMAE treatment for 2 or 3 days on these cancer cells were evaluated by MTT assay

Article Snippet: In the drug release assays, 0.5 mg/mL HuNb TROP2-HSA -MMAE was incubated with 5 μg/mL cathepsin B (MCE, HY-P78682) at pH 6.0 for different time.

Techniques: In Vitro, Activity Assay, Staining, Labeling, Expressing, Flow Cytometry, MTT Assay

Journal: Journal of Nanobiotechnology

Article Title: TROP2-directed nanobody-drug conjugate elicited potent antitumor effect in pancreatic cancer

doi: 10.1186/s12951-023-02183-9

Figure Lengend Snippet: Cell apoptosis induced by HuNb TROP2-HSA -MMAE. A The effect of HuNb TROP2-HSA -MMAE on apoptosis of BxPC-3 and PK-59 cells was detected by Annexin V-FITC/PI staining. Annexin V + PI − (Q4) represents early apoptotic cells and Annexin V + PI + (Q2) represents late apoptotic cells. B A statistical analysis of three replicate experiments of ( A ). The proportion of apoptotic cells is the sum of Q2 and Q4. C Western Blot demonstrated the expressions of apoptosis-related proteins in pancreatic cancer cells after 72 h of treatment of HuNb TROP2-HSA -MMAE

Article Snippet: In the drug release assays, 0.5 mg/mL HuNb TROP2-HSA -MMAE was incubated with 5 μg/mL cathepsin B (MCE, HY-P78682) at pH 6.0 for different time.

Techniques: Staining, Western Blot

Journal: Journal of Nanobiotechnology

Article Title: TROP2-directed nanobody-drug conjugate elicited potent antitumor effect in pancreatic cancer

doi: 10.1186/s12951-023-02183-9

Figure Lengend Snippet: In vivo distribution and metabolism of HuNb TROP2-HSA -MMAE. A Living imaging after intravenous injection of 200 μg Cy7-HuNb TROP2-HSA -MMAE for different times. B Fluorescence distribution in major organs at 24 h post-injection. C Quantitative analysis of the fluorescence intensity in ( B )

Article Snippet: In the drug release assays, 0.5 mg/mL HuNb TROP2-HSA -MMAE was incubated with 5 μg/mL cathepsin B (MCE, HY-P78682) at pH 6.0 for different time.

Techniques: In Vivo, Imaging, Injection, Fluorescence

Journal: Journal of Nanobiotechnology

Article Title: TROP2-directed nanobody-drug conjugate elicited potent antitumor effect in pancreatic cancer

doi: 10.1186/s12951-023-02183-9

Figure Lengend Snippet: In vivo antitumor activity of HuNb TROP2-HSA -MMAE. A Tumor volume during 4 weeks of treatment with different doses of HuNb TROP2-HSA -MMAE. B Survival curves of mice receiving various treatments. C Each line represents the tumor volume for an individual mouse. D H&E staining and IHC for Ki-67, cleaved caspase-3 and cleaved PARP of tumor tissues

Article Snippet: In the drug release assays, 0.5 mg/mL HuNb TROP2-HSA -MMAE was incubated with 5 μg/mL cathepsin B (MCE, HY-P78682) at pH 6.0 for different time.

Techniques: In Vivo, Activity Assay, Staining

Journal: Frontiers in Microbiology

Article Title: Akkermansia muciniphila Alters Gut Microbiota and Immune System to Improve Cardiovascular Diseases in Murine Model

doi: 10.3389/fmicb.2022.906920

Figure Lengend Snippet: A. muciniphila regulates the expression of immune factors. (A) The levels of MMP-1, MMP-9, CTSB, and CTSL in abdominal aortic tissue were analyzed by ELISA. (B,C) The expression of IL-33 in abdominal aortic tissue was analyzed by IHC (scale bar = 25 μm; the magnification is 400×). (D) qRT-PCR was used to analyze IL-33 gene expression in abdominal aortic tissue. (E) The expression of IL-33 protein in abdominal aortic tissue was analyzed by Western blot. (F) The levels of IL-4, IL-6, IL-10, IL-17A, IFN-γ, and C-reactive protein (CRP) in peripheral blood were determined by ELISA. (G) The expression of PCNA, OPN, and α-SMA protein in abdominal aortic aneurysm tissue were detected by Western blot. Data were shown as means ± SD and analyzed by two-way ANOVA ( n = 10 mice/group). *Compared with the control group, P < 0.05; # compared with the AAA group, P < 0.05.

Article Snippet: According to the manufacturer’s instructions, the levels of MMP-1 (ml037721, Shanghai Meilian, Shanghai, China), MMP-9 (CSB-E08007m, CUSABIO, Wuhan, China), CTSB (CSB-EL006185MO, CUSABIO, Wuhan, China), CTSL (E-EL-M0251c, Elabscience, Wuhan, China), IL-4 (CSB-E04634m, CUSABIO, Wuhan, China), IL-6 (CSB-E04639m, CUSABIO, Wuhan, China), IL-10 (CSB-E04594m, CUSABIO, Wuhan, China), IL-17A (CSB-E04608m, CUSABIO, Wuhan, China), IFN-γ (CSB-E04578m, CUSABIO, Wuhan, China), and C-reactive protein (CRP, CSB-E07922r, CUSABIO, Wuhan, China) were analyzed by ELISA assay.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Gene Expression, Western Blot, Control