Journal: Biochemistry and Biophysics Reports
Article Title: Crystallization and initial X-ray diffraction analysis of the multi-domain Brucella blue light-activated histidine kinase LOV-HK in its illuminated state
Figure Lengend Snippet: LOV-HK crystals. (A) Screen hit #1, obtained with 30% ( v/v ) MPD, 5% ( w/v ) PEG 4000, 0.1 M Hepes, pH 7.5 (Jena Bioscience JBScreen Classic 7 Solution C4). (B) Screen hit #2, obtained with 5% ( v/v ) isopropanol, 0.1 M Hepes, pH 7.5 (Jena Bioscience JBScreen Classic 9 Solution A1). (C) Screen hit #3, obtained with 10% ( w/v ) PEG 4000, 20% ( v/v ) isopropanol, 0.1 M Hepes, pH 7.5 (Jena Bioscience JBScreen Classic 3 Solution A5). (D) Improved crystals obtained after optimization of screen hit #1. (E) Mounted crystal at the PROXIMA-2A beamline, ready for diffraction.
Article Snippet: Initial crystallization trials were performed at 5.3 mg ml−1 in 96-well sitting-drop vapour-diffusion Greiner 609120 plates (Monroe, North Carolina, USA) using a Honeybee 963 robot (Digilab, Marlborough, Massachusetts, USA) and the following crystallization kits: Jena Bioscience JBScreen Classic and Pentaerythritol (Jena, Germany), and Hampton Research Crystal Screen, Crystal Screen 2, PEG/Ion, PEG/Ion 2, PEGRx 1 and PEGRx 2 (Aliso Viejo, California, USA).