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    Jena Bioscience jbscreen classic
    LOV-HK crystals. (A) Screen hit #1, obtained with 30% ( v/v ) MPD, 5% ( w/v ) PEG 4000, 0.1 M Hepes, pH 7.5 <t>(Jena</t> Bioscience <t>JBScreen</t> Classic 7 Solution C4). (B) Screen hit #2, obtained with 5% ( v/v ) isopropanol, 0.1 M Hepes, pH 7.5 (Jena Bioscience JBScreen Classic 9 Solution A1). (C) Screen hit #3, obtained with 10% ( w/v ) PEG 4000, 20% ( v/v ) isopropanol, 0.1 M Hepes, pH 7.5 (Jena Bioscience JBScreen Classic 3 Solution A5). (D) Improved crystals obtained after optimization of screen hit #1. (E) Mounted crystal at the PROXIMA-2A beamline, ready for diffraction.
    Jbscreen Classic, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    LOV-HK crystals. (A) Screen hit #1, obtained with 30% ( v/v ) MPD, 5% ( w/v ) PEG 4000, 0.1 M Hepes, pH 7.5 (Jena Bioscience JBScreen Classic 7 Solution C4). (B) Screen hit #2, obtained with 5% ( v/v ) isopropanol, 0.1 M Hepes, pH 7.5 (Jena Bioscience JBScreen Classic 9 Solution A1). (C) Screen hit #3, obtained with 10% ( w/v ) PEG 4000, 20% ( v/v ) isopropanol, 0.1 M Hepes, pH 7.5 (Jena Bioscience JBScreen Classic 3 Solution A5). (D) Improved crystals obtained after optimization of screen hit #1. (E) Mounted crystal at the PROXIMA-2A beamline, ready for diffraction.

    Journal: Biochemistry and Biophysics Reports

    Article Title: Crystallization and initial X-ray diffraction analysis of the multi-domain Brucella blue light-activated histidine kinase LOV-HK in its illuminated state

    doi: 10.1016/j.bbrep.2018.09.005

    Figure Lengend Snippet: LOV-HK crystals. (A) Screen hit #1, obtained with 30% ( v/v ) MPD, 5% ( w/v ) PEG 4000, 0.1 M Hepes, pH 7.5 (Jena Bioscience JBScreen Classic 7 Solution C4). (B) Screen hit #2, obtained with 5% ( v/v ) isopropanol, 0.1 M Hepes, pH 7.5 (Jena Bioscience JBScreen Classic 9 Solution A1). (C) Screen hit #3, obtained with 10% ( w/v ) PEG 4000, 20% ( v/v ) isopropanol, 0.1 M Hepes, pH 7.5 (Jena Bioscience JBScreen Classic 3 Solution A5). (D) Improved crystals obtained after optimization of screen hit #1. (E) Mounted crystal at the PROXIMA-2A beamline, ready for diffraction.

    Article Snippet: Initial crystallization trials were performed at 5.3 mg ml−1 in 96-well sitting-drop vapour-diffusion Greiner 609120 plates (Monroe, North Carolina, USA) using a Honeybee 963 robot (Digilab, Marlborough, Massachusetts, USA) and the following crystallization kits: Jena Bioscience JBScreen Classic and Pentaerythritol (Jena, Germany), and Hampton Research Crystal Screen, Crystal Screen 2, PEG/Ion, PEG/Ion 2, PEGRx 1 and PEGRx 2 (Aliso Viejo, California, USA).

    Techniques: