crude oligomers Search Results


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  • 98
    Zymo Research oligo clean concentrator
    Oligo Clean Concentrator, supplied by Zymo Research, used in various techniques. Bioz Stars score: 98/100, based on 92 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 98 stars, based on 92 article reviews
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    99
    New England Biolabs oligo dt cellulose beads
    In vitro selection of RNA elements that mediate cap-independent (CI) translation ( a ) A library of human genomic DNA fragments was inserted into a DNA cassette containing all of the sequence information necessary for <t>mRNA</t> display. For each selection round, the dsDNA pool was in vitro transcribed into ssRNA, conjugated to a DNA-puromycin linker, and translated in vitro . Uncapped mRNA sequences that initiate translation of an intact ORF become covalently linked to a His-6 protein affinity tag encoded in the RNA message. Functional molecules are recovered, reverse transcribed, and amplified by PCR to generate the DNA for the next selection cycle. ( b ) RNA-protein fusion molecules are generated via the natural peptidyl transferase activity of the ribosome. ( c ) Selection progress was monitored by measuring the fraction of S 35 -labeled fusion molecules recovered from the <t>oligo-dT</t> and Ni-NTA affinity columns.
    Oligo Dt Cellulose Beads, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Eurofins crude oligodeoxynucleotides
    In vitro selection of RNA elements that mediate cap-independent (CI) translation ( a ) A library of human genomic DNA fragments was inserted into a DNA cassette containing all of the sequence information necessary for <t>mRNA</t> display. For each selection round, the dsDNA pool was in vitro transcribed into ssRNA, conjugated to a DNA-puromycin linker, and translated in vitro . Uncapped mRNA sequences that initiate translation of an intact ORF become covalently linked to a His-6 protein affinity tag encoded in the RNA message. Functional molecules are recovered, reverse transcribed, and amplified by PCR to generate the DNA for the next selection cycle. ( b ) RNA-protein fusion molecules are generated via the natural peptidyl transferase activity of the ribosome. ( c ) Selection progress was monitored by measuring the fraction of S 35 -labeled fusion molecules recovered from the <t>oligo-dT</t> and Ni-NTA affinity columns.
    Crude Oligodeoxynucleotides, supplied by Eurofins, used in various techniques. Bioz Stars score: 89/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 89 stars, based on 12 article reviews
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    85
    Eppendorf AG poros oligo r2
    In vitro selection of RNA elements that mediate cap-independent (CI) translation ( a ) A library of human genomic DNA fragments was inserted into a DNA cassette containing all of the sequence information necessary for <t>mRNA</t> display. For each selection round, the dsDNA pool was in vitro transcribed into ssRNA, conjugated to a DNA-puromycin linker, and translated in vitro . Uncapped mRNA sequences that initiate translation of an intact ORF become covalently linked to a His-6 protein affinity tag encoded in the RNA message. Functional molecules are recovered, reverse transcribed, and amplified by PCR to generate the DNA for the next selection cycle. ( b ) RNA-protein fusion molecules are generated via the natural peptidyl transferase activity of the ribosome. ( c ) Selection progress was monitored by measuring the fraction of S 35 -labeled fusion molecules recovered from the <t>oligo-dT</t> and Ni-NTA affinity columns.
    Poros Oligo R2, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 85 stars, based on 2 article reviews
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    99
    Millipore high performance liquid chromatography rp hplc
    In vitro selection of RNA elements that mediate cap-independent (CI) translation ( a ) A library of human genomic DNA fragments was inserted into a DNA cassette containing all of the sequence information necessary for <t>mRNA</t> display. For each selection round, the dsDNA pool was in vitro transcribed into ssRNA, conjugated to a DNA-puromycin linker, and translated in vitro . Uncapped mRNA sequences that initiate translation of an intact ORF become covalently linked to a His-6 protein affinity tag encoded in the RNA message. Functional molecules are recovered, reverse transcribed, and amplified by PCR to generate the DNA for the next selection cycle. ( b ) RNA-protein fusion molecules are generated via the natural peptidyl transferase activity of the ribosome. ( c ) Selection progress was monitored by measuring the fraction of S 35 -labeled fusion molecules recovered from the <t>oligo-dT</t> and Ni-NTA affinity columns.
    High Performance Liquid Chromatography Rp Hplc, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Nacalai phase hplc
    In vitro selection of RNA elements that mediate cap-independent (CI) translation ( a ) A library of human genomic DNA fragments was inserted into a DNA cassette containing all of the sequence information necessary for <t>mRNA</t> display. For each selection round, the dsDNA pool was in vitro transcribed into ssRNA, conjugated to a DNA-puromycin linker, and translated in vitro . Uncapped mRNA sequences that initiate translation of an intact ORF become covalently linked to a His-6 protein affinity tag encoded in the RNA message. Functional molecules are recovered, reverse transcribed, and amplified by PCR to generate the DNA for the next selection cycle. ( b ) RNA-protein fusion molecules are generated via the natural peptidyl transferase activity of the ribosome. ( c ) Selection progress was monitored by measuring the fraction of S 35 -labeled fusion molecules recovered from the <t>oligo-dT</t> and Ni-NTA affinity columns.
    Phase Hplc, supplied by Nacalai, used in various techniques. Bioz Stars score: 92/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 25 article reviews
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    91
    BioShop oligo dt cellulose
    The association of importin-α1 with poly(A)-RNA in growing cells is regulated by stress. Crude extracts prepared for controls (ethanol, EtOH) and <t>DEM-treated</t> cells were incubated with <t>oligo-(dT)-cellulose</t> as described in Materials and methods . Aliquots of the start (St, 10%) and eluted (E, 100%) material were analyzed by Western blotting with different antibodies as indicated. The relative binding was calculated for importin-α1 and HuR; results were normalized to control samples. Data for three independent experiments are shown as average +SEM. Student’s t-test was applied to identify significant differences between control and DEM-treated samples; *, p
    Oligo Dt Cellulose, supplied by BioShop, used in various techniques. Bioz Stars score: 91/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    ATUM sperm dna
    The association of importin-α1 with poly(A)-RNA in growing cells is regulated by stress. Crude extracts prepared for controls (ethanol, EtOH) and <t>DEM-treated</t> cells were incubated with <t>oligo-(dT)-cellulose</t> as described in Materials and methods . Aliquots of the start (St, 10%) and eluted (E, 100%) material were analyzed by Western blotting with different antibodies as indicated. The relative binding was calculated for importin-α1 and HuR; results were normalized to control samples. Data for three independent experiments are shown as average +SEM. Student’s t-test was applied to identify significant differences between control and DEM-treated samples; *, p
    Sperm Dna, supplied by ATUM, used in various techniques. Bioz Stars score: 92/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    MACHEREY NAGEL anion exchange hplc
    The association of importin-α1 with poly(A)-RNA in growing cells is regulated by stress. Crude extracts prepared for controls (ethanol, EtOH) and <t>DEM-treated</t> cells were incubated with <t>oligo-(dT)-cellulose</t> as described in Materials and methods . Aliquots of the start (St, 10%) and eluted (E, 100%) material were analyzed by Western blotting with different antibodies as indicated. The relative binding was calculated for importin-α1 and HuR; results were normalized to control samples. Data for three independent experiments are shown as average +SEM. Student’s t-test was applied to identify significant differences between control and DEM-treated samples; *, p
    Anion Exchange Hplc, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    In vitro selection of RNA elements that mediate cap-independent (CI) translation ( a ) A library of human genomic DNA fragments was inserted into a DNA cassette containing all of the sequence information necessary for mRNA display. For each selection round, the dsDNA pool was in vitro transcribed into ssRNA, conjugated to a DNA-puromycin linker, and translated in vitro . Uncapped mRNA sequences that initiate translation of an intact ORF become covalently linked to a His-6 protein affinity tag encoded in the RNA message. Functional molecules are recovered, reverse transcribed, and amplified by PCR to generate the DNA for the next selection cycle. ( b ) RNA-protein fusion molecules are generated via the natural peptidyl transferase activity of the ribosome. ( c ) Selection progress was monitored by measuring the fraction of S 35 -labeled fusion molecules recovered from the oligo-dT and Ni-NTA affinity columns.

    Journal: Nature methods

    Article Title: Genome-Wide Profiling of Cap-Independent Translation Enhancing Elements in the Human Genome

    doi: 10.1038/nmeth.2522

    Figure Lengend Snippet: In vitro selection of RNA elements that mediate cap-independent (CI) translation ( a ) A library of human genomic DNA fragments was inserted into a DNA cassette containing all of the sequence information necessary for mRNA display. For each selection round, the dsDNA pool was in vitro transcribed into ssRNA, conjugated to a DNA-puromycin linker, and translated in vitro . Uncapped mRNA sequences that initiate translation of an intact ORF become covalently linked to a His-6 protein affinity tag encoded in the RNA message. Functional molecules are recovered, reverse transcribed, and amplified by PCR to generate the DNA for the next selection cycle. ( b ) RNA-protein fusion molecules are generated via the natural peptidyl transferase activity of the ribosome. ( c ) Selection progress was monitored by measuring the fraction of S 35 -labeled fusion molecules recovered from the oligo-dT and Ni-NTA affinity columns.

    Article Snippet: The mRNA-peptide fusion molecules were purified from the crude lysate using oligo (dT)-cellulose beads (NEB) and reverse transcribed with SuperScriptII (Invitrogen) by extending the DNA primer (5' TTTTTTTTTTTTTTTATCC ACTTCCATGATGATGGT) with dNTPs.

    Techniques: In Vitro, Selection, Sequencing, Functional Assay, Amplification, Polymerase Chain Reaction, Generated, Activity Assay, Labeling

    The association of importin-α1 with poly(A)-RNA in growing cells is regulated by stress. Crude extracts prepared for controls (ethanol, EtOH) and DEM-treated cells were incubated with oligo-(dT)-cellulose as described in Materials and methods . Aliquots of the start (St, 10%) and eluted (E, 100%) material were analyzed by Western blotting with different antibodies as indicated. The relative binding was calculated for importin-α1 and HuR; results were normalized to control samples. Data for three independent experiments are shown as average +SEM. Student’s t-test was applied to identify significant differences between control and DEM-treated samples; *, p

    Journal: PLoS ONE

    Article Title: Identification of Novel Stress Granule Components That Are Involved in Nuclear Transport

    doi: 10.1371/journal.pone.0068356

    Figure Lengend Snippet: The association of importin-α1 with poly(A)-RNA in growing cells is regulated by stress. Crude extracts prepared for controls (ethanol, EtOH) and DEM-treated cells were incubated with oligo-(dT)-cellulose as described in Materials and methods . Aliquots of the start (St, 10%) and eluted (E, 100%) material were analyzed by Western blotting with different antibodies as indicated. The relative binding was calculated for importin-α1 and HuR; results were normalized to control samples. Data for three independent experiments are shown as average +SEM. Student’s t-test was applied to identify significant differences between control and DEM-treated samples; *, p

    Article Snippet: Crude extracts generated from control and DEM-treated cells were incubated with oligo-(dT) cellulose, and bound material was analyzed by Western blotting.

    Techniques: Incubation, Western Blot, Binding Assay