Journal: Cell Adhesion & Migration
Article Title: Orphan G protein-coupled receptor GPRC5A modulates integrin β1-mediated epithelial cell adhesion
Figure Lengend Snippet: Knock-out of GPRC5A results in reduced β1 integrin protein expression. (A) Western blot demonstrating a reduction in the total integrin β1 (ItgB1) protein in GPRC5A knock-out MDA-MB-231 cells grown on Collagen I matrix (0.1 mg/mL) for 48 h. KO#1 and KO#2 refer to 2 independent small guide RNAs used for a CRISPR/Cas9-mediated GPRC5A knockout. (B) Quantification of integrin β1 protein from 3 independent experiments like the one shown in (A). Black lines show mean values. Error bars represent SEM. Statistical significance was evaluated using the Mann-Whitney non-parametric test. (C) RT-qPCR results demonstrate that, in contrast to the protein, the amount of the ItgB1 transcript is increased in GPRC5A knock-out cells. Statistical significance was evaluated using ANOVA with Tukey post-hoc test. *, p
Article Snippet: CRISPR/Cas9-mediated GPRC5A knock-out Oligonucleotides encoding guide RNAs were purchased from SigmaAldrich.
Techniques: Knock-Out, Expressing, Western Blot, Multiple Displacement Amplification, CRISPR, MANN-WHITNEY, Quantitative RT-PCR