crispr arrays primers Search Results


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  • 99
    Integrated DNA Technologies crispr rna oligonucleotides
    Crispr Rna Oligonucleotides, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 99/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore crispr cas9 mediated gprc5a knock out oligonucleotides encoding guide rnas
    Knock-out of <t>GPRC5A</t> results in reduced β1 integrin protein expression. (A) Western blot demonstrating a reduction in the total integrin β1 (ItgB1) protein in GPRC5A knock-out MDA-MB-231 cells grown on Collagen I matrix (0.1 mg/mL) for 48 h. KO#1 and KO#2 refer to 2 independent small guide <t>RNAs</t> used for a <t>CRISPR/Cas9-mediated</t> GPRC5A knockout. (B) Quantification of integrin β1 protein from 3 independent experiments like the one shown in (A). Black lines show mean values. Error bars represent SEM. Statistical significance was evaluated using the Mann-Whitney non-parametric test. (C) RT-qPCR results demonstrate that, in contrast to the protein, the amount of the ItgB1 transcript is increased in GPRC5A knock-out cells. Statistical significance was evaluated using ANOVA with Tukey post-hoc test. *, p
    Crispr Cas9 Mediated Gprc5a Knock Out Oligonucleotides Encoding Guide Rnas, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 11 article reviews
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    84
    biomers.net synthetic crispr repeat oligonucleotides
    Knock-out of <t>GPRC5A</t> results in reduced β1 integrin protein expression. (A) Western blot demonstrating a reduction in the total integrin β1 (ItgB1) protein in GPRC5A knock-out MDA-MB-231 cells grown on Collagen I matrix (0.1 mg/mL) for 48 h. KO#1 and KO#2 refer to 2 independent small guide <t>RNAs</t> used for a <t>CRISPR/Cas9-mediated</t> GPRC5A knockout. (B) Quantification of integrin β1 protein from 3 independent experiments like the one shown in (A). Black lines show mean values. Error bars represent SEM. Statistical significance was evaluated using the Mann-Whitney non-parametric test. (C) RT-qPCR results demonstrate that, in contrast to the protein, the amount of the ItgB1 transcript is increased in GPRC5A knock-out cells. Statistical significance was evaluated using ANOVA with Tukey post-hoc test. *, p
    Synthetic Crispr Repeat Oligonucleotides, supplied by biomers.net, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Elim Bio crispr cas9 mediated gene disruption sgrna oligonucleotides
    Knock-out of <t>GPRC5A</t> results in reduced β1 integrin protein expression. (A) Western blot demonstrating a reduction in the total integrin β1 (ItgB1) protein in GPRC5A knock-out MDA-MB-231 cells grown on Collagen I matrix (0.1 mg/mL) for 48 h. KO#1 and KO#2 refer to 2 independent small guide <t>RNAs</t> used for a <t>CRISPR/Cas9-mediated</t> GPRC5A knockout. (B) Quantification of integrin β1 protein from 3 independent experiments like the one shown in (A). Black lines show mean values. Error bars represent SEM. Statistical significance was evaluated using the Mann-Whitney non-parametric test. (C) RT-qPCR results demonstrate that, in contrast to the protein, the amount of the ItgB1 transcript is increased in GPRC5A knock-out cells. Statistical significance was evaluated using ANOVA with Tukey post-hoc test. *, p
    Crispr Cas9 Mediated Gene Disruption Sgrna Oligonucleotides, supplied by Elim Bio, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GenScript crispr guide library cloninga 69nt guide oligo pool
    Knock-out of <t>GPRC5A</t> results in reduced β1 integrin protein expression. (A) Western blot demonstrating a reduction in the total integrin β1 (ItgB1) protein in GPRC5A knock-out MDA-MB-231 cells grown on Collagen I matrix (0.1 mg/mL) for 48 h. KO#1 and KO#2 refer to 2 independent small guide <t>RNAs</t> used for a <t>CRISPR/Cas9-mediated</t> GPRC5A knockout. (B) Quantification of integrin β1 protein from 3 independent experiments like the one shown in (A). Black lines show mean values. Error bars represent SEM. Statistical significance was evaluated using the Mann-Whitney non-parametric test. (C) RT-qPCR results demonstrate that, in contrast to the protein, the amount of the ItgB1 transcript is increased in GPRC5A knock-out cells. Statistical significance was evaluated using ANOVA with Tukey post-hoc test. *, p
    Crispr Guide Library Cloninga 69nt Guide Oligo Pool, supplied by GenScript, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Addgene inc primers crispr cas9 plasmid
    Knock-out of <t>GPRC5A</t> results in reduced β1 integrin protein expression. (A) Western blot demonstrating a reduction in the total integrin β1 (ItgB1) protein in GPRC5A knock-out MDA-MB-231 cells grown on Collagen I matrix (0.1 mg/mL) for 48 h. KO#1 and KO#2 refer to 2 independent small guide <t>RNAs</t> used for a <t>CRISPR/Cas9-mediated</t> GPRC5A knockout. (B) Quantification of integrin β1 protein from 3 independent experiments like the one shown in (A). Black lines show mean values. Error bars represent SEM. Statistical significance was evaluated using the Mann-Whitney non-parametric test. (C) RT-qPCR results demonstrate that, in contrast to the protein, the amount of the ItgB1 transcript is increased in GPRC5A knock-out cells. Statistical significance was evaluated using ANOVA with Tukey post-hoc test. *, p
    Primers Crispr Cas9 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Knock-out of GPRC5A results in reduced β1 integrin protein expression. (A) Western blot demonstrating a reduction in the total integrin β1 (ItgB1) protein in GPRC5A knock-out MDA-MB-231 cells grown on Collagen I matrix (0.1 mg/mL) for 48 h. KO#1 and KO#2 refer to 2 independent small guide RNAs used for a CRISPR/Cas9-mediated GPRC5A knockout. (B) Quantification of integrin β1 protein from 3 independent experiments like the one shown in (A). Black lines show mean values. Error bars represent SEM. Statistical significance was evaluated using the Mann-Whitney non-parametric test. (C) RT-qPCR results demonstrate that, in contrast to the protein, the amount of the ItgB1 transcript is increased in GPRC5A knock-out cells. Statistical significance was evaluated using ANOVA with Tukey post-hoc test. *, p

    Journal: Cell Adhesion & Migration

    Article Title: Orphan G protein-coupled receptor GPRC5A modulates integrin β1-mediated epithelial cell adhesion

    doi: 10.1080/19336918.2016.1245264

    Figure Lengend Snippet: Knock-out of GPRC5A results in reduced β1 integrin protein expression. (A) Western blot demonstrating a reduction in the total integrin β1 (ItgB1) protein in GPRC5A knock-out MDA-MB-231 cells grown on Collagen I matrix (0.1 mg/mL) for 48 h. KO#1 and KO#2 refer to 2 independent small guide RNAs used for a CRISPR/Cas9-mediated GPRC5A knockout. (B) Quantification of integrin β1 protein from 3 independent experiments like the one shown in (A). Black lines show mean values. Error bars represent SEM. Statistical significance was evaluated using the Mann-Whitney non-parametric test. (C) RT-qPCR results demonstrate that, in contrast to the protein, the amount of the ItgB1 transcript is increased in GPRC5A knock-out cells. Statistical significance was evaluated using ANOVA with Tukey post-hoc test. *, p

    Article Snippet: CRISPR/Cas9-mediated GPRC5A knock-out Oligonucleotides encoding guide RNAs were purchased from SigmaAldrich.

    Techniques: Knock-Out, Expressing, Western Blot, Multiple Displacement Amplification, CRISPR, MANN-WHITNEY, Quantitative RT-PCR