cosmogel his-accept Nacalai Search Results


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  • 90
    Nacalai cosmogel his accept
    Cosmogel His Accept, supplied by Nacalai, used in various techniques. Bioz Stars score: 90/100, based on 227 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Nacalai cosmogel his accept beads
    Cosmogel His Accept Beads, supplied by Nacalai, used in various techniques. Bioz Stars score: 86/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Nacalai cosmogel his accept resin
    Cosmogel His Accept Resin, supplied by Nacalai, used in various techniques. Bioz Stars score: 86/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Nacalai metal affinity resin cosmogel his accept
    Metal Affinity Resin Cosmogel His Accept, supplied by Nacalai, used in various techniques. Bioz Stars score: 92/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Nacalai cosmogel his accept column
    SDS-PAGE analysis of the protein fractions collected at each step of purification of HBx(101−136)-(GS) 5 -Bcl-x L . Lane 1, molecular weight marker; lane 2, supernatant; lane 3, His 6 -HBx(101−136)-(GS) 5 -Bcl-x L purified by a <t>COSMOGEL</t> His-accept column; lane 4, His 6 -HBx(101−136)-(GS) 5 -Bcl-x L after dialysis; lane 5, HBx(101−136)-(GS) 5 -Bcl-x L after cleavage of the His 6 -tag by thrombin; lane 6, HBx(101−136)-(GS) 5 -Bcl-x L purified by a Resource Q anion exchange column; lanes 7–9, purified HBx(101−136)-(GS) 5 -Bcl-x L (1-, 3- and 5-µg loadings, respectively).
    Cosmogel His Accept Column, supplied by Nacalai, used in various techniques. Bioz Stars score: 90/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Nacalai his tag affinity column
    SDS-PAGE analysis of the protein fractions collected at each step of purification of HBx(101−136)-(GS) 5 -Bcl-x L . Lane 1, molecular weight marker; lane 2, supernatant; lane 3, His 6 -HBx(101−136)-(GS) 5 -Bcl-x L purified by a <t>COSMOGEL</t> His-accept column; lane 4, His 6 -HBx(101−136)-(GS) 5 -Bcl-x L after dialysis; lane 5, HBx(101−136)-(GS) 5 -Bcl-x L after cleavage of the His 6 -tag by thrombin; lane 6, HBx(101−136)-(GS) 5 -Bcl-x L purified by a Resource Q anion exchange column; lanes 7–9, purified HBx(101−136)-(GS) 5 -Bcl-x L (1-, 3- and 5-µg loadings, respectively).
    His Tag Affinity Column, supplied by Nacalai, used in various techniques. Bioz Stars score: 88/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Nacalai his nickel affinity chromatography
    SDS-PAGE analysis of the protein fractions collected at each step of purification of HBx(101−136)-(GS) 5 -Bcl-x L . Lane 1, molecular weight marker; lane 2, supernatant; lane 3, His 6 -HBx(101−136)-(GS) 5 -Bcl-x L purified by a <t>COSMOGEL</t> His-accept column; lane 4, His 6 -HBx(101−136)-(GS) 5 -Bcl-x L after dialysis; lane 5, HBx(101−136)-(GS) 5 -Bcl-x L after cleavage of the His 6 -tag by thrombin; lane 6, HBx(101−136)-(GS) 5 -Bcl-x L purified by a Resource Q anion exchange column; lanes 7–9, purified HBx(101−136)-(GS) 5 -Bcl-x L (1-, 3- and 5-µg loadings, respectively).
    His Nickel Affinity Chromatography, supplied by Nacalai, used in various techniques. Bioz Stars score: 90/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Nacalai ni affinity resin
    SDS-PAGE analysis of the protein fractions collected at each step of purification of HBx(101−136)-(GS) 5 -Bcl-x L . Lane 1, molecular weight marker; lane 2, supernatant; lane 3, His 6 -HBx(101−136)-(GS) 5 -Bcl-x L purified by a <t>COSMOGEL</t> His-accept column; lane 4, His 6 -HBx(101−136)-(GS) 5 -Bcl-x L after dialysis; lane 5, HBx(101−136)-(GS) 5 -Bcl-x L after cleavage of the His 6 -tag by thrombin; lane 6, HBx(101−136)-(GS) 5 -Bcl-x L purified by a Resource Q anion exchange column; lanes 7–9, purified HBx(101−136)-(GS) 5 -Bcl-x L (1-, 3- and 5-µg loadings, respectively).
    Ni Affinity Resin, supplied by Nacalai, used in various techniques. Bioz Stars score: 91/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Nacalai ni2 nta affinity column
    SDS-PAGE analysis of the protein fractions collected at each step of purification of HBx(101−136)-(GS) 5 -Bcl-x L . Lane 1, molecular weight marker; lane 2, supernatant; lane 3, His 6 -HBx(101−136)-(GS) 5 -Bcl-x L purified by a <t>COSMOGEL</t> His-accept column; lane 4, His 6 -HBx(101−136)-(GS) 5 -Bcl-x L after dialysis; lane 5, HBx(101−136)-(GS) 5 -Bcl-x L after cleavage of the His 6 -tag by thrombin; lane 6, HBx(101−136)-(GS) 5 -Bcl-x L purified by a Resource Q anion exchange column; lanes 7–9, purified HBx(101−136)-(GS) 5 -Bcl-x L (1-, 3- and 5-µg loadings, respectively).
    Ni2 Nta Affinity Column, supplied by Nacalai, used in various techniques. Bioz Stars score: 90/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Nacalai ni agarose
    SDS-PAGE analysis of the protein fractions collected at each step of purification of HBx(101−136)-(GS) 5 -Bcl-x L . Lane 1, molecular weight marker; lane 2, supernatant; lane 3, His 6 -HBx(101−136)-(GS) 5 -Bcl-x L purified by a <t>COSMOGEL</t> His-accept column; lane 4, His 6 -HBx(101−136)-(GS) 5 -Bcl-x L after dialysis; lane 5, HBx(101−136)-(GS) 5 -Bcl-x L after cleavage of the His 6 -tag by thrombin; lane 6, HBx(101−136)-(GS) 5 -Bcl-x L purified by a Resource Q anion exchange column; lanes 7–9, purified HBx(101−136)-(GS) 5 -Bcl-x L (1-, 3- and 5-µg loadings, respectively).
    Ni Agarose, supplied by Nacalai, used in various techniques. Bioz Stars score: 91/100, based on 64 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Nacalai ni nta column
    SDS-PAGE analysis of the protein fractions collected at each step of purification of HBx(101−136)-(GS) 5 -Bcl-x L . Lane 1, molecular weight marker; lane 2, supernatant; lane 3, His 6 -HBx(101−136)-(GS) 5 -Bcl-x L purified by a <t>COSMOGEL</t> His-accept column; lane 4, His 6 -HBx(101−136)-(GS) 5 -Bcl-x L after dialysis; lane 5, HBx(101−136)-(GS) 5 -Bcl-x L after cleavage of the His 6 -tag by thrombin; lane 6, HBx(101−136)-(GS) 5 -Bcl-x L purified by a Resource Q anion exchange column; lanes 7–9, purified HBx(101−136)-(GS) 5 -Bcl-x L (1-, 3- and 5-µg loadings, respectively).
    Ni Nta Column, supplied by Nacalai, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Nacalai nitrilotriacetic acid nta affinity column
    SDS-PAGE analysis of the protein fractions collected at each step of purification of HBx(101−136)-(GS) 5 -Bcl-x L . Lane 1, molecular weight marker; lane 2, supernatant; lane 3, His 6 -HBx(101−136)-(GS) 5 -Bcl-x L purified by a <t>COSMOGEL</t> His-accept column; lane 4, His 6 -HBx(101−136)-(GS) 5 -Bcl-x L after dialysis; lane 5, HBx(101−136)-(GS) 5 -Bcl-x L after cleavage of the His 6 -tag by thrombin; lane 6, HBx(101−136)-(GS) 5 -Bcl-x L purified by a Resource Q anion exchange column; lanes 7–9, purified HBx(101−136)-(GS) 5 -Bcl-x L (1-, 3- and 5-µg loadings, respectively).
    Nitrilotriacetic Acid Nta Affinity Column, supplied by Nacalai, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Nacalai ni2 sepharose resin
    SDS-PAGE analysis of the protein fractions collected at each step of purification of HBx(101−136)-(GS) 5 -Bcl-x L . Lane 1, molecular weight marker; lane 2, supernatant; lane 3, His 6 -HBx(101−136)-(GS) 5 -Bcl-x L purified by a <t>COSMOGEL</t> His-accept column; lane 4, His 6 -HBx(101−136)-(GS) 5 -Bcl-x L after dialysis; lane 5, HBx(101−136)-(GS) 5 -Bcl-x L after cleavage of the His 6 -tag by thrombin; lane 6, HBx(101−136)-(GS) 5 -Bcl-x L purified by a Resource Q anion exchange column; lanes 7–9, purified HBx(101−136)-(GS) 5 -Bcl-x L (1-, 3- and 5-µg loadings, respectively).
    Ni2 Sepharose Resin, supplied by Nacalai, used in various techniques. Bioz Stars score: 90/100, based on 74 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Nacalai nitrilotriacetic acid affinity column
    SDS-PAGE analysis of the protein fractions collected at each step of purification of HBx(101−136)-(GS) 5 -Bcl-x L . Lane 1, molecular weight marker; lane 2, supernatant; lane 3, His 6 -HBx(101−136)-(GS) 5 -Bcl-x L purified by a <t>COSMOGEL</t> His-accept column; lane 4, His 6 -HBx(101−136)-(GS) 5 -Bcl-x L after dialysis; lane 5, HBx(101−136)-(GS) 5 -Bcl-x L after cleavage of the His 6 -tag by thrombin; lane 6, HBx(101−136)-(GS) 5 -Bcl-x L purified by a Resource Q anion exchange column; lanes 7–9, purified HBx(101−136)-(GS) 5 -Bcl-x L (1-, 3- and 5-µg loadings, respectively).
    Nitrilotriacetic Acid Affinity Column, supplied by Nacalai, used in various techniques. Bioz Stars score: 92/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Nacalai nickel agarose column
    SDS-PAGE analysis of the protein fractions collected at each step of purification of HBx(101−136)-(GS) 5 -Bcl-x L . Lane 1, molecular weight marker; lane 2, supernatant; lane 3, His 6 -HBx(101−136)-(GS) 5 -Bcl-x L purified by a <t>COSMOGEL</t> His-accept column; lane 4, His 6 -HBx(101−136)-(GS) 5 -Bcl-x L after dialysis; lane 5, HBx(101−136)-(GS) 5 -Bcl-x L after cleavage of the His 6 -tag by thrombin; lane 6, HBx(101−136)-(GS) 5 -Bcl-x L purified by a Resource Q anion exchange column; lanes 7–9, purified HBx(101−136)-(GS) 5 -Bcl-x L (1-, 3- and 5-µg loadings, respectively).
    Nickel Agarose Column, supplied by Nacalai, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    SDS-PAGE analysis of the protein fractions collected at each step of purification of HBx(101−136)-(GS) 5 -Bcl-x L . Lane 1, molecular weight marker; lane 2, supernatant; lane 3, His 6 -HBx(101−136)-(GS) 5 -Bcl-x L purified by a COSMOGEL His-accept column; lane 4, His 6 -HBx(101−136)-(GS) 5 -Bcl-x L after dialysis; lane 5, HBx(101−136)-(GS) 5 -Bcl-x L after cleavage of the His 6 -tag by thrombin; lane 6, HBx(101−136)-(GS) 5 -Bcl-x L purified by a Resource Q anion exchange column; lanes 7–9, purified HBx(101−136)-(GS) 5 -Bcl-x L (1-, 3- and 5-µg loadings, respectively).

    Journal: Biochemistry and Biophysics Reports

    Article Title: Expression, purification and characterization of hepatitis B virus X protein BH3-like motif-linker-Bcl-xL fusion protein for structural studies

    doi: 10.1016/j.bbrep.2016.12.006

    Figure Lengend Snippet: SDS-PAGE analysis of the protein fractions collected at each step of purification of HBx(101−136)-(GS) 5 -Bcl-x L . Lane 1, molecular weight marker; lane 2, supernatant; lane 3, His 6 -HBx(101−136)-(GS) 5 -Bcl-x L purified by a COSMOGEL His-accept column; lane 4, His 6 -HBx(101−136)-(GS) 5 -Bcl-x L after dialysis; lane 5, HBx(101−136)-(GS) 5 -Bcl-x L after cleavage of the His 6 -tag by thrombin; lane 6, HBx(101−136)-(GS) 5 -Bcl-x L purified by a Resource Q anion exchange column; lanes 7–9, purified HBx(101−136)-(GS) 5 -Bcl-x L (1-, 3- and 5-µg loadings, respectively).

    Article Snippet: The supernatant containing the His6 -tagged protein, which was obtained by centrifugation, was loaded onto a COSMOGEL His-accept column (3–4 mL bed volume) (Nacalai Tesque Inc.).

    Techniques: SDS Page, Purification, Molecular Weight, Marker