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  • 86
    Thermo Fisher control sirna
    Control Sirna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/control sirna/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    control sirna - by Bioz Stars, 2021-09
    86/100 stars
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    86
    Santa Cruz Biotechnology control sirna
    Knockdown of <t>TIM-1</t> expression impairs JEV entry and infection. A549 cells were seeded in the 12-well plate, and they were transfected with TIM-1-specific <t>siRNA</t> of different concentrations or control siRNA at 50 nM using lipofectamine RNAiMAX Reagent. At 48 h post-transfection, cells were infected with JEV NJ2008 (MOI = 1) for 24 h. ( A ) Cell lysates were harvested to detect the level of JEV NS1 protein and cellular TIM-1 expression by Western blot analysis, using GAPDH as an internal control. One representative experiment out of three is shown; ( B ) production of progeny virions were determined by titering the supernatants of indicated cells in BHK-21 cells. Data are presented as mean ± SD from three independent experiments; ( C ) cells were transfected with TIM-1 siRNA or control siRNA for 48 h before being challenged with JEV NJ2008 (MOI of 10). At 24 h post-infection, cells were fixed and stained for E glycoprotein (red) and TIM-1 (green), followed by confocal microscopy analysis. Scale bars, 20 µm. A549 cells were transfected with TIM-1 siRNA or control siRNA for 48 h; ( D ) cells were incubated with JEV NJ2008 (MOI of 5) at 4 °C for 30 min and then washed with PBS three times; ( E ) cells were incubated with JEV NJ2008 with a MOI of 5 at 4 °C for 1 h and washed with PBS three times, then shifted to 37 °C for 15 min to allow JEV entry. Cells were treated with proteinase K (1 mg/mL) to remove non-internalized virions. Total RNA was extracted and used for quantification of JEV RNA, the efficiency of TIM-1 silencing and JEV attachment and entry were detected by qRT-PCR. Data are presented as mean ± SD from three independent experiments using t-test. ** p
    Control Sirna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/control sirna/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    control sirna - by Bioz Stars, 2021-09
    86/100 stars
      Buy from Supplier

    94
    Thermo Fisher sirna
    Small interference RNA <t>(siRNA)-mediated</t> depletion of <t>QKI</t> splice variants in astrocytoma (U343) cell lines. A. Exon-intron structure of the quaking gene and the regions targeted by small interfering RNA. siQKI-tot was designed to suppress all QKI splice variants (QKI-tot), siQKI-5 silences only splice variant 5 (QKI-5), and siQKI-7 silences splice variants 7 and 7b (QKI-7). The sequences included in each siRNA cocktail are listed in Table 2 . B. Percentage of remaining messenger RNA expression levels for QKI-tot, QKI-5 and QKI-7, are shown after silencing with siQKI-tot, siQKI-5 or siQKI-7. Asterisk (*) indicates significant deviation in the mRNA levels compared with the untreated (CTL) groups (p-value
    Sirna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sirna/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sirna - by Bioz Stars, 2021-09
    94/100 stars
      Buy from Supplier

    86
    Thermo Fisher negative control sirna
    Small interference RNA <t>(siRNA)-mediated</t> depletion of <t>QKI</t> splice variants in astrocytoma (U343) cell lines. A. Exon-intron structure of the quaking gene and the regions targeted by small interfering RNA. siQKI-tot was designed to suppress all QKI splice variants (QKI-tot), siQKI-5 silences only splice variant 5 (QKI-5), and siQKI-7 silences splice variants 7 and 7b (QKI-7). The sequences included in each siRNA cocktail are listed in Table 2 . B. Percentage of remaining messenger RNA expression levels for QKI-tot, QKI-5 and QKI-7, are shown after silencing with siQKI-tot, siQKI-5 or siQKI-7. Asterisk (*) indicates significant deviation in the mRNA levels compared with the untreated (CTL) groups (p-value
    Negative Control Sirna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/negative control sirna/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    negative control sirna - by Bioz Stars, 2021-09
    86/100 stars
      Buy from Supplier

    Image Search Results


    Knockdown of TIM-1 expression impairs JEV entry and infection. A549 cells were seeded in the 12-well plate, and they were transfected with TIM-1-specific siRNA of different concentrations or control siRNA at 50 nM using lipofectamine RNAiMAX Reagent. At 48 h post-transfection, cells were infected with JEV NJ2008 (MOI = 1) for 24 h. ( A ) Cell lysates were harvested to detect the level of JEV NS1 protein and cellular TIM-1 expression by Western blot analysis, using GAPDH as an internal control. One representative experiment out of three is shown; ( B ) production of progeny virions were determined by titering the supernatants of indicated cells in BHK-21 cells. Data are presented as mean ± SD from three independent experiments; ( C ) cells were transfected with TIM-1 siRNA or control siRNA for 48 h before being challenged with JEV NJ2008 (MOI of 10). At 24 h post-infection, cells were fixed and stained for E glycoprotein (red) and TIM-1 (green), followed by confocal microscopy analysis. Scale bars, 20 µm. A549 cells were transfected with TIM-1 siRNA or control siRNA for 48 h; ( D ) cells were incubated with JEV NJ2008 (MOI of 5) at 4 °C for 30 min and then washed with PBS three times; ( E ) cells were incubated with JEV NJ2008 with a MOI of 5 at 4 °C for 1 h and washed with PBS three times, then shifted to 37 °C for 15 min to allow JEV entry. Cells were treated with proteinase K (1 mg/mL) to remove non-internalized virions. Total RNA was extracted and used for quantification of JEV RNA, the efficiency of TIM-1 silencing and JEV attachment and entry were detected by qRT-PCR. Data are presented as mean ± SD from three independent experiments using t-test. ** p

    Journal: Viruses

    Article Title: TIM-1 Promotes Japanese Encephalitis Virus Entry and Infection

    doi: 10.3390/v10110630

    Figure Lengend Snippet: Knockdown of TIM-1 expression impairs JEV entry and infection. A549 cells were seeded in the 12-well plate, and they were transfected with TIM-1-specific siRNA of different concentrations or control siRNA at 50 nM using lipofectamine RNAiMAX Reagent. At 48 h post-transfection, cells were infected with JEV NJ2008 (MOI = 1) for 24 h. ( A ) Cell lysates were harvested to detect the level of JEV NS1 protein and cellular TIM-1 expression by Western blot analysis, using GAPDH as an internal control. One representative experiment out of three is shown; ( B ) production of progeny virions were determined by titering the supernatants of indicated cells in BHK-21 cells. Data are presented as mean ± SD from three independent experiments; ( C ) cells were transfected with TIM-1 siRNA or control siRNA for 48 h before being challenged with JEV NJ2008 (MOI of 10). At 24 h post-infection, cells were fixed and stained for E glycoprotein (red) and TIM-1 (green), followed by confocal microscopy analysis. Scale bars, 20 µm. A549 cells were transfected with TIM-1 siRNA or control siRNA for 48 h; ( D ) cells were incubated with JEV NJ2008 (MOI of 5) at 4 °C for 30 min and then washed with PBS three times; ( E ) cells were incubated with JEV NJ2008 with a MOI of 5 at 4 °C for 1 h and washed with PBS three times, then shifted to 37 °C for 15 min to allow JEV entry. Cells were treated with proteinase K (1 mg/mL) to remove non-internalized virions. Total RNA was extracted and used for quantification of JEV RNA, the efficiency of TIM-1 silencing and JEV attachment and entry were detected by qRT-PCR. Data are presented as mean ± SD from three independent experiments using t-test. ** p

    Article Snippet: Pools of TIM-1 siRNA (sc-61691) and control siRNA (sc-37007) were purchased from SantaCruz.

    Techniques: Expressing, Infection, Transfection, Western Blot, Staining, Confocal Microscopy, Incubation, Quantitative RT-PCR

    Small interference RNA (siRNA)-mediated depletion of QKI splice variants in astrocytoma (U343) cell lines. A. Exon-intron structure of the quaking gene and the regions targeted by small interfering RNA. siQKI-tot was designed to suppress all QKI splice variants (QKI-tot), siQKI-5 silences only splice variant 5 (QKI-5), and siQKI-7 silences splice variants 7 and 7b (QKI-7). The sequences included in each siRNA cocktail are listed in Table 2 . B. Percentage of remaining messenger RNA expression levels for QKI-tot, QKI-5 and QKI-7, are shown after silencing with siQKI-tot, siQKI-5 or siQKI-7. Asterisk (*) indicates significant deviation in the mRNA levels compared with the untreated (CTL) groups (p-value

    Journal: PLoS ONE

    Article Title: QKI-7 Regulates Expression of Interferon-Related Genes in Human Astrocyte Glioma Cells

    doi: 10.1371/journal.pone.0013079

    Figure Lengend Snippet: Small interference RNA (siRNA)-mediated depletion of QKI splice variants in astrocytoma (U343) cell lines. A. Exon-intron structure of the quaking gene and the regions targeted by small interfering RNA. siQKI-tot was designed to suppress all QKI splice variants (QKI-tot), siQKI-5 silences only splice variant 5 (QKI-5), and siQKI-7 silences splice variants 7 and 7b (QKI-7). The sequences included in each siRNA cocktail are listed in Table 2 . B. Percentage of remaining messenger RNA expression levels for QKI-tot, QKI-5 and QKI-7, are shown after silencing with siQKI-tot, siQKI-5 or siQKI-7. Asterisk (*) indicates significant deviation in the mRNA levels compared with the untreated (CTL) groups (p-value

    Article Snippet: All siRNA sequences are shown in , and the positions of the sequences with respect to the QKI gene structure are shown in . siRNA for GAPDH (Ambion Silencer™ GAPDH siRNA) was used as a positive control for transfection.

    Techniques: Small Interfering RNA, Variant Assay, RNA Expression, CTL Assay, Significance Assay