control gfp expression vector Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher lipofectamine 2000
    Lipofectamine 2000, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipofectamine 2000/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lipofectamine 2000 - by Bioz Stars, 2021-07
    99/100 stars
      Buy from Supplier

    86
    Thermo Fisher gfp
    The Ank9 N-terminus is necessary for localization to Golgi- and ER-positive cellular fractions. Lysates of HeLa cells transfected to express <t>GFP-tagged</t> Ank9, Ank9ΔF-box, or Ank9 47–422 for 4 h or 16 h were subjected to density gradient fractionation. Western blots of nine successive fractions were screened with GFP, <t>GM130,</t> and calreticulin antibodies to confirm the Ank9 proteins’ subcellular trafficking patterns. Results are representative of two experiments with similar results.
    Gfp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gfp/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gfp - by Bioz Stars, 2021-07
    86/100 stars
      Buy from Supplier

    pcr  (TaKaRa)
    99
    TaKaRa pcr
    The Ank9 N-terminus is necessary for localization to Golgi- and ER-positive cellular fractions. Lysates of HeLa cells transfected to express <t>GFP-tagged</t> Ank9, Ank9ΔF-box, or Ank9 47–422 for 4 h or 16 h were subjected to density gradient fractionation. Western blots of nine successive fractions were screened with GFP, <t>GM130,</t> and calreticulin antibodies to confirm the Ank9 proteins’ subcellular trafficking patterns. Results are representative of two experiments with similar results.
    Pcr, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr/product/TaKaRa
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pcr - by Bioz Stars, 2021-07
    99/100 stars
      Buy from Supplier

    99
    Addgene inc pspcas9 bb 2a gfp
    The Ank9 N-terminus is necessary for localization to Golgi- and ER-positive cellular fractions. Lysates of HeLa cells transfected to express <t>GFP-tagged</t> Ank9, Ank9ΔF-box, or Ank9 47–422 for 4 h or 16 h were subjected to density gradient fractionation. Western blots of nine successive fractions were screened with GFP, <t>GM130,</t> and calreticulin antibodies to confirm the Ank9 proteins’ subcellular trafficking patterns. Results are representative of two experiments with similar results.
    Pspcas9 Bb 2a Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pspcas9 bb 2a gfp/product/Addgene inc
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pspcas9 bb 2a gfp - by Bioz Stars, 2021-07
    99/100 stars
      Buy from Supplier

    Image Search Results


    The Ank9 N-terminus is necessary for localization to Golgi- and ER-positive cellular fractions. Lysates of HeLa cells transfected to express GFP-tagged Ank9, Ank9ΔF-box, or Ank9 47–422 for 4 h or 16 h were subjected to density gradient fractionation. Western blots of nine successive fractions were screened with GFP, GM130, and calreticulin antibodies to confirm the Ank9 proteins’ subcellular trafficking patterns. Results are representative of two experiments with similar results.

    Journal: Cellular microbiology

    Article Title: Orientia tsutsugamushi Ank9 is a multifunctional effector that utilizes a novel GRIP-like Golgi localization domain for Golgi-to-endoplasmic reticulum trafficking and interacts with host COPB2

    doi: 10.1111/cmi.12727

    Figure Lengend Snippet: The Ank9 N-terminus is necessary for localization to Golgi- and ER-positive cellular fractions. Lysates of HeLa cells transfected to express GFP-tagged Ank9, Ank9ΔF-box, or Ank9 47–422 for 4 h or 16 h were subjected to density gradient fractionation. Western blots of nine successive fractions were screened with GFP, GM130, and calreticulin antibodies to confirm the Ank9 proteins’ subcellular trafficking patterns. Results are representative of two experiments with similar results.

    Article Snippet: Equal volumes of fractions 1 to 9 were immunoblotted using GM130 (1:500, BD Biosciences), calreticulin (1:4000, Sigma Aldrich), and GFP (1:1000, Thermo Fisher) antibodies.

    Techniques: Transfection, Fractionation, Western Blot

    Ectopically expressed Ank9 localizes to and disrupts the morphology of the ER. HeLa cells expressing GFP-Ank9 (A) or GFP (B) were fixed and screened at 16 h post transfection with GFP antibody and antibody against one of the ER lumenal markers, calreticulin or PDI, or ER transmembrane proteins, calnexin or derlin-1, prior to examination by confocal microscopy. Representative fluorescence images of cells viewed for GFP, ER marker, and merged images plus DAPI, which stains the nucleus, are presented. White arrows denote representative points of GFP and ER marker signal colocalization. Because the cell cycles of the HeLa cells were not synchronized, the timing by which GFP-Ank9 localizes to and disrupts the ER could not be discerned. Results are representative of three independent experiments with similar results. Scale bars, 20 μm.

    Journal: Cellular microbiology

    Article Title: Orientia tsutsugamushi Ank9 is a multifunctional effector that utilizes a novel GRIP-like Golgi localization domain for Golgi-to-endoplasmic reticulum trafficking and interacts with host COPB2

    doi: 10.1111/cmi.12727

    Figure Lengend Snippet: Ectopically expressed Ank9 localizes to and disrupts the morphology of the ER. HeLa cells expressing GFP-Ank9 (A) or GFP (B) were fixed and screened at 16 h post transfection with GFP antibody and antibody against one of the ER lumenal markers, calreticulin or PDI, or ER transmembrane proteins, calnexin or derlin-1, prior to examination by confocal microscopy. Representative fluorescence images of cells viewed for GFP, ER marker, and merged images plus DAPI, which stains the nucleus, are presented. White arrows denote representative points of GFP and ER marker signal colocalization. Because the cell cycles of the HeLa cells were not synchronized, the timing by which GFP-Ank9 localizes to and disrupts the ER could not be discerned. Results are representative of three independent experiments with similar results. Scale bars, 20 μm.

    Article Snippet: Equal volumes of fractions 1 to 9 were immunoblotted using GM130 (1:500, BD Biosciences), calreticulin (1:4000, Sigma Aldrich), and GFP (1:1000, Thermo Fisher) antibodies.

    Techniques: Expressing, Transfection, Confocal Microscopy, Fluorescence, Marker

    Ectopically expressed Ank9 exhibits temporal localization phenotypes that are consistent with Golgi-to-ER retrograde trafficking. Serum starved HeLa cells were transfected to express GFP-Ank9 (A and B). At 4, 8, or 12 h post transfection, the cells were fixed and examined by confocal microscopy (A–C). In some cases, fixed cells were screened with antibody against the cis-Golgi marker, GM130, or the ER protein, derlin-1 prior to confocal microscopic analyses (B). Representative fluorescence images of cells viewed for GFP; GM130 or derlin-1; and merged images plus DAPI are presented. White arrows in B denote representative points of GFP and organelle marker signal colocalization. (A) GFP-Ank9 exhibits subcellular localization patterns reminiscent of the Golgi at 4 h and destabilized ER at 8 and 12 h. (B) Immunofluorescent labeling confirms that GFP-Ank9 localizes to the Golgi at 4 h and subsequently traffics to the ER at 8 h and 12 h. Destabilization of GFP-Ank9-positive Golgi and ER is apparent at the 8 and 12 h time points. Representative cells exhibiting GFP-Ank9 Golgi like (Golgi) and ER-like (ER) subcellular localization patterns are denoted. (C and D) GFP-Ank9 does not require an intact Golgi to localize to the Golgi and ER. HeLa cells were treated with brefeldin A (BFA) or vehicle control. At 4 h, the cells were immunolabeled with GM130 antibody, stained with DAPI, and visualized using confocal microscopy to confirm that BFA destabilizes the Golgi (C). (D) Serum starved HeLa cells were treated with BFA- or vehicle control just prior to transfection with GFP-Ank9 plasmid. The cells were examined for GFP-Ank9 subcellular localization at 4, 8, and 16 h. Data are presented as the percentage of cells in which GFP-Ank9 signal was diffuse in the cytosol (Diffuse), localized to and disrupted the Golgi (Golgi), or localized to and altered the morphology of the ER to yield vesicular or ring-like structures (ER vesicles/rings). Results are representative of at least two separate experiments performed in triplicate, each yielding similar results. Scale bars, 20 μm. ns, not significant.

    Journal: Cellular microbiology

    Article Title: Orientia tsutsugamushi Ank9 is a multifunctional effector that utilizes a novel GRIP-like Golgi localization domain for Golgi-to-endoplasmic reticulum trafficking and interacts with host COPB2

    doi: 10.1111/cmi.12727

    Figure Lengend Snippet: Ectopically expressed Ank9 exhibits temporal localization phenotypes that are consistent with Golgi-to-ER retrograde trafficking. Serum starved HeLa cells were transfected to express GFP-Ank9 (A and B). At 4, 8, or 12 h post transfection, the cells were fixed and examined by confocal microscopy (A–C). In some cases, fixed cells were screened with antibody against the cis-Golgi marker, GM130, or the ER protein, derlin-1 prior to confocal microscopic analyses (B). Representative fluorescence images of cells viewed for GFP; GM130 or derlin-1; and merged images plus DAPI are presented. White arrows in B denote representative points of GFP and organelle marker signal colocalization. (A) GFP-Ank9 exhibits subcellular localization patterns reminiscent of the Golgi at 4 h and destabilized ER at 8 and 12 h. (B) Immunofluorescent labeling confirms that GFP-Ank9 localizes to the Golgi at 4 h and subsequently traffics to the ER at 8 h and 12 h. Destabilization of GFP-Ank9-positive Golgi and ER is apparent at the 8 and 12 h time points. Representative cells exhibiting GFP-Ank9 Golgi like (Golgi) and ER-like (ER) subcellular localization patterns are denoted. (C and D) GFP-Ank9 does not require an intact Golgi to localize to the Golgi and ER. HeLa cells were treated with brefeldin A (BFA) or vehicle control. At 4 h, the cells were immunolabeled with GM130 antibody, stained with DAPI, and visualized using confocal microscopy to confirm that BFA destabilizes the Golgi (C). (D) Serum starved HeLa cells were treated with BFA- or vehicle control just prior to transfection with GFP-Ank9 plasmid. The cells were examined for GFP-Ank9 subcellular localization at 4, 8, and 16 h. Data are presented as the percentage of cells in which GFP-Ank9 signal was diffuse in the cytosol (Diffuse), localized to and disrupted the Golgi (Golgi), or localized to and altered the morphology of the ER to yield vesicular or ring-like structures (ER vesicles/rings). Results are representative of at least two separate experiments performed in triplicate, each yielding similar results. Scale bars, 20 μm. ns, not significant.

    Article Snippet: Equal volumes of fractions 1 to 9 were immunoblotted using GM130 (1:500, BD Biosciences), calreticulin (1:4000, Sigma Aldrich), and GFP (1:1000, Thermo Fisher) antibodies.

    Techniques: Transfection, Confocal Microscopy, Marker, Fluorescence, Labeling, Immunolabeling, Staining, Plasmid Preparation

    GFP-Ank9 localization to and perturbation of Golgi and ER morphology is F-box-independent. HeLa cells expressing GFP-Ank9ΔF-box were fixed, screened with GM130, derlin-1, and LAMP-1 antibodies, stained with DAPI, and examined by confocal microscopy. Representative fluorescence images of cells displaying GFP-Ank9ΔF-box Golgi-like (Golgi) and ER-like (ER) subcellular localization patterns viewed for GFP, organelle marker, and merged images plus DAPI are presented. Scale bars, 20 μm. Results shown are representative of three experiments with similar results.

    Journal: Cellular microbiology

    Article Title: Orientia tsutsugamushi Ank9 is a multifunctional effector that utilizes a novel GRIP-like Golgi localization domain for Golgi-to-endoplasmic reticulum trafficking and interacts with host COPB2

    doi: 10.1111/cmi.12727

    Figure Lengend Snippet: GFP-Ank9 localization to and perturbation of Golgi and ER morphology is F-box-independent. HeLa cells expressing GFP-Ank9ΔF-box were fixed, screened with GM130, derlin-1, and LAMP-1 antibodies, stained with DAPI, and examined by confocal microscopy. Representative fluorescence images of cells displaying GFP-Ank9ΔF-box Golgi-like (Golgi) and ER-like (ER) subcellular localization patterns viewed for GFP, organelle marker, and merged images plus DAPI are presented. Scale bars, 20 μm. Results shown are representative of three experiments with similar results.

    Article Snippet: Equal volumes of fractions 1 to 9 were immunoblotted using GM130 (1:500, BD Biosciences), calreticulin (1:4000, Sigma Aldrich), and GFP (1:1000, Thermo Fisher) antibodies.

    Techniques: Expressing, Staining, Confocal Microscopy, Fluorescence, Marker