Journal: PLoS ONE
Article Title: Neutrophils Increase or Reduce Parasite Burden in Trypanosoma cruzi-Infected Macrophages, Depending on Host Strain: Role of Neutrophil Elastase
Figure Lengend Snippet: Neutrophil elastase potentiates the microbicidal response of T. cruzi in vitro . (A) Parasites released in infected C57BL/6 macrophages cultures alone or in presence of solvent (DMSO), control inhibitor (collagenase inhibitor Z-Pro-D-Leu-AAPV-D-Ala-NHOH; used at 10 µg/ml) or NE inhibitor (MeOSuc-AAPV-cmk; used at 10 µg/ml). (B) Neutrophil elastase (100 ng/ml) was added to infected cultured macrophages in the presence or absence of selective iNOS inhibitor (L-NIL). The number of trypomastigotes was counted in a Neubauer chamber after 7 days of culture. Production of TNF-α and NO released into the supernatant by T. cruzi infected macrophages after 24 h incubation in medium alone or in the presence of neutrophil elastase, at 100 ng/ml. Measurements were performed in triplicates of three different experiments. Statistical significance was determined by ANOVA (A) or t test (B).
Article Snippet: Monolayers were also treated with the specific NE inhibitor methoxysuccinyl-Ala-Ala-Pro-Val-chloromethylketone (MeoSuc-AAPV-CMK; Calbiochen-Novabiochem, La Jolla, CA), control collagenase inhibitor Z-Pro-D-Leu-D-Ala-NHOH (Calbiochem-Novabiochem), both at 10 µg/ml, or equivalent dosage of solvent (DMSO) alone.
Techniques: In Vitro, Infection, Cell Culture, Incubation