concentrated protein Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95
    Millipore protein expression recombinant kh1
    <t>KH1-KH23</t> ligation. ( a ) HPLC traces showing the formation of the MPAA thioester intermediate and ligated KH123 product (identified by cartoons) over time. Diphenyl column was used to resolve the KH23 and KH123 peaks. The unidentified ‘*’ impurity, which is at constant concentration during the reaction, provides a serendipitous control. Experiments were repeated three times. ( b ) (Left) Expected and measured masses of the reagents and product of the ligation. For the KH3 domain a 98% 15 N labelling has been assumed based on the level of enrichment of the 15 N nitrogen source. (Right) Reconstituted electrospray mass spectrum showing the de-convoluted molecular mass of the ligated KH123. ( c ) Superimposition of the backbone amide of 1 H{ 15 N} correlation experiments recorded on the KH3 and ligated KH3-only 15 N labelled KH123 proteins, indicating that the KH3 domain is correctly folded and assembled in the KH123 construct and highlighting the high quality of the NMR data. ( d ) The values of the 15 N longitudinal relaxation time of backbone amide groups in the ligated KH3 (black dots, this study) and of the isolated domains (orange dots, as previously reported 38 ) plotted along the protein sequence.
    Protein Expression Recombinant Kh1, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protein expression recombinant kh1/product/Millipore
    Average 95 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    protein expression recombinant kh1 - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    90
    Thermo Fisher protein concentrator pes
    <t>KH1-KH23</t> ligation. ( a ) HPLC traces showing the formation of the MPAA thioester intermediate and ligated KH123 product (identified by cartoons) over time. Diphenyl column was used to resolve the KH23 and KH123 peaks. The unidentified ‘*’ impurity, which is at constant concentration during the reaction, provides a serendipitous control. Experiments were repeated three times. ( b ) (Left) Expected and measured masses of the reagents and product of the ligation. For the KH3 domain a 98% 15 N labelling has been assumed based on the level of enrichment of the 15 N nitrogen source. (Right) Reconstituted electrospray mass spectrum showing the de-convoluted molecular mass of the ligated KH123. ( c ) Superimposition of the backbone amide of 1 H{ 15 N} correlation experiments recorded on the KH3 and ligated KH3-only 15 N labelled KH123 proteins, indicating that the KH3 domain is correctly folded and assembled in the KH123 construct and highlighting the high quality of the NMR data. ( d ) The values of the 15 N longitudinal relaxation time of backbone amide groups in the ligated KH3 (black dots, this study) and of the isolated domains (orange dots, as previously reported 38 ) plotted along the protein sequence.
    Protein Concentrator Pes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protein concentrator pes/product/Thermo Fisher
    Average 90 stars, based on 23 article reviews
    Price from $9.99 to $1999.99
    protein concentrator pes - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    79
    Millipore protein concentrator
    <t>KH1-KH23</t> ligation. ( a ) HPLC traces showing the formation of the MPAA thioester intermediate and ligated KH123 product (identified by cartoons) over time. Diphenyl column was used to resolve the KH23 and KH123 peaks. The unidentified ‘*’ impurity, which is at constant concentration during the reaction, provides a serendipitous control. Experiments were repeated three times. ( b ) (Left) Expected and measured masses of the reagents and product of the ligation. For the KH3 domain a 98% 15 N labelling has been assumed based on the level of enrichment of the 15 N nitrogen source. (Right) Reconstituted electrospray mass spectrum showing the de-convoluted molecular mass of the ligated KH123. ( c ) Superimposition of the backbone amide of 1 H{ 15 N} correlation experiments recorded on the KH3 and ligated KH3-only 15 N labelled KH123 proteins, indicating that the KH3 domain is correctly folded and assembled in the KH123 construct and highlighting the high quality of the NMR data. ( d ) The values of the 15 N longitudinal relaxation time of backbone amide groups in the ligated KH3 (black dots, this study) and of the isolated domains (orange dots, as previously reported 38 ) plotted along the protein sequence.
    Protein Concentrator, supplied by Millipore, used in various techniques. Bioz Stars score: 79/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protein concentrator/product/Millipore
    Average 79 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    protein concentrator - by Bioz Stars, 2020-02
    79/100 stars
      Buy from Supplier

    83
    Thermo Fisher protein concentrators
    <t>KH1-KH23</t> ligation. ( a ) HPLC traces showing the formation of the MPAA thioester intermediate and ligated KH123 product (identified by cartoons) over time. Diphenyl column was used to resolve the KH23 and KH123 peaks. The unidentified ‘*’ impurity, which is at constant concentration during the reaction, provides a serendipitous control. Experiments were repeated three times. ( b ) (Left) Expected and measured masses of the reagents and product of the ligation. For the KH3 domain a 98% 15 N labelling has been assumed based on the level of enrichment of the 15 N nitrogen source. (Right) Reconstituted electrospray mass spectrum showing the de-convoluted molecular mass of the ligated KH123. ( c ) Superimposition of the backbone amide of 1 H{ 15 N} correlation experiments recorded on the KH3 and ligated KH3-only 15 N labelled KH123 proteins, indicating that the KH3 domain is correctly folded and assembled in the KH123 construct and highlighting the high quality of the NMR data. ( d ) The values of the 15 N longitudinal relaxation time of backbone amide groups in the ligated KH3 (black dots, this study) and of the isolated domains (orange dots, as previously reported 38 ) plotted along the protein sequence.
    Protein Concentrators, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 83/100, based on 127 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protein concentrators/product/Thermo Fisher
    Average 83 stars, based on 127 article reviews
    Price from $9.99 to $1999.99
    protein concentrators - by Bioz Stars, 2020-02
    83/100 stars
      Buy from Supplier

    90
    Bio-Rad protein assay dye reagent concentrate
    <t>KH1-KH23</t> ligation. ( a ) HPLC traces showing the formation of the MPAA thioester intermediate and ligated KH123 product (identified by cartoons) over time. Diphenyl column was used to resolve the KH23 and KH123 peaks. The unidentified ‘*’ impurity, which is at constant concentration during the reaction, provides a serendipitous control. Experiments were repeated three times. ( b ) (Left) Expected and measured masses of the reagents and product of the ligation. For the KH3 domain a 98% 15 N labelling has been assumed based on the level of enrichment of the 15 N nitrogen source. (Right) Reconstituted electrospray mass spectrum showing the de-convoluted molecular mass of the ligated KH123. ( c ) Superimposition of the backbone amide of 1 H{ 15 N} correlation experiments recorded on the KH3 and ligated KH3-only 15 N labelled KH123 proteins, indicating that the KH3 domain is correctly folded and assembled in the KH123 construct and highlighting the high quality of the NMR data. ( d ) The values of the 15 N longitudinal relaxation time of backbone amide groups in the ligated KH3 (black dots, this study) and of the isolated domains (orange dots, as previously reported 38 ) plotted along the protein sequence.
    Protein Assay Dye Reagent Concentrate, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 167 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protein assay dye reagent concentrate/product/Bio-Rad
    Average 90 stars, based on 167 article reviews
    Price from $9.99 to $1999.99
    protein assay dye reagent concentrate - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    85
    Millipore centricon protein concentrator
    <t>KH1-KH23</t> ligation. ( a ) HPLC traces showing the formation of the MPAA thioester intermediate and ligated KH123 product (identified by cartoons) over time. Diphenyl column was used to resolve the KH23 and KH123 peaks. The unidentified ‘*’ impurity, which is at constant concentration during the reaction, provides a serendipitous control. Experiments were repeated three times. ( b ) (Left) Expected and measured masses of the reagents and product of the ligation. For the KH3 domain a 98% 15 N labelling has been assumed based on the level of enrichment of the 15 N nitrogen source. (Right) Reconstituted electrospray mass spectrum showing the de-convoluted molecular mass of the ligated KH123. ( c ) Superimposition of the backbone amide of 1 H{ 15 N} correlation experiments recorded on the KH3 and ligated KH3-only 15 N labelled KH123 proteins, indicating that the KH3 domain is correctly folded and assembled in the KH123 construct and highlighting the high quality of the NMR data. ( d ) The values of the 15 N longitudinal relaxation time of backbone amide groups in the ligated KH3 (black dots, this study) and of the isolated domains (orange dots, as previously reported 38 ) plotted along the protein sequence.
    Centricon Protein Concentrator, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/centricon protein concentrator/product/Millipore
    Average 85 stars, based on 43 article reviews
    Price from $9.99 to $1999.99
    centricon protein concentrator - by Bioz Stars, 2020-02
    85/100 stars
      Buy from Supplier

    80
    Millipore centrifuge protein concentrator
    <t>KH1-KH23</t> ligation. ( a ) HPLC traces showing the formation of the MPAA thioester intermediate and ligated KH123 product (identified by cartoons) over time. Diphenyl column was used to resolve the KH23 and KH123 peaks. The unidentified ‘*’ impurity, which is at constant concentration during the reaction, provides a serendipitous control. Experiments were repeated three times. ( b ) (Left) Expected and measured masses of the reagents and product of the ligation. For the KH3 domain a 98% 15 N labelling has been assumed based on the level of enrichment of the 15 N nitrogen source. (Right) Reconstituted electrospray mass spectrum showing the de-convoluted molecular mass of the ligated KH123. ( c ) Superimposition of the backbone amide of 1 H{ 15 N} correlation experiments recorded on the KH3 and ligated KH3-only 15 N labelled KH123 proteins, indicating that the KH3 domain is correctly folded and assembled in the KH123 construct and highlighting the high quality of the NMR data. ( d ) The values of the 15 N longitudinal relaxation time of backbone amide groups in the ligated KH3 (black dots, this study) and of the isolated domains (orange dots, as previously reported 38 ) plotted along the protein sequence.
    Centrifuge Protein Concentrator, supplied by Millipore, used in various techniques. Bioz Stars score: 80/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/centrifuge protein concentrator/product/Millipore
    Average 80 stars, based on 21 article reviews
    Price from $9.99 to $1999.99
    centrifuge protein concentrator - by Bioz Stars, 2020-02
    80/100 stars
      Buy from Supplier

    91
    Fisher Scientific pierce protein concentrator
    <t>KH1-KH23</t> ligation. ( a ) HPLC traces showing the formation of the MPAA thioester intermediate and ligated KH123 product (identified by cartoons) over time. Diphenyl column was used to resolve the KH23 and KH123 peaks. The unidentified ‘*’ impurity, which is at constant concentration during the reaction, provides a serendipitous control. Experiments were repeated three times. ( b ) (Left) Expected and measured masses of the reagents and product of the ligation. For the KH3 domain a 98% 15 N labelling has been assumed based on the level of enrichment of the 15 N nitrogen source. (Right) Reconstituted electrospray mass spectrum showing the de-convoluted molecular mass of the ligated KH123. ( c ) Superimposition of the backbone amide of 1 H{ 15 N} correlation experiments recorded on the KH3 and ligated KH3-only 15 N labelled KH123 proteins, indicating that the KH3 domain is correctly folded and assembled in the KH123 construct and highlighting the high quality of the NMR data. ( d ) The values of the 15 N longitudinal relaxation time of backbone amide groups in the ligated KH3 (black dots, this study) and of the isolated domains (orange dots, as previously reported 38 ) plotted along the protein sequence.
    Pierce Protein Concentrator, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 91/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pierce protein concentrator/product/Fisher Scientific
    Average 91 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    pierce protein concentrator - by Bioz Stars, 2020-02
    91/100 stars
      Buy from Supplier

    79
    Thermo Fisher icon protein concentrators
    <t>KH1-KH23</t> ligation. ( a ) HPLC traces showing the formation of the MPAA thioester intermediate and ligated KH123 product (identified by cartoons) over time. Diphenyl column was used to resolve the KH23 and KH123 peaks. The unidentified ‘*’ impurity, which is at constant concentration during the reaction, provides a serendipitous control. Experiments were repeated three times. ( b ) (Left) Expected and measured masses of the reagents and product of the ligation. For the KH3 domain a 98% 15 N labelling has been assumed based on the level of enrichment of the 15 N nitrogen source. (Right) Reconstituted electrospray mass spectrum showing the de-convoluted molecular mass of the ligated KH123. ( c ) Superimposition of the backbone amide of 1 H{ 15 N} correlation experiments recorded on the KH3 and ligated KH3-only 15 N labelled KH123 proteins, indicating that the KH3 domain is correctly folded and assembled in the KH123 construct and highlighting the high quality of the NMR data. ( d ) The values of the 15 N longitudinal relaxation time of backbone amide groups in the ligated KH3 (black dots, this study) and of the isolated domains (orange dots, as previously reported 38 ) plotted along the protein sequence.
    Icon Protein Concentrators, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/icon protein concentrators/product/Thermo Fisher
    Average 79 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    icon protein concentrators - by Bioz Stars, 2020-02
    79/100 stars
      Buy from Supplier

    77
    Thermo Fisher icontm protein concentrators
    <t>KH1-KH23</t> ligation. ( a ) HPLC traces showing the formation of the MPAA thioester intermediate and ligated KH123 product (identified by cartoons) over time. Diphenyl column was used to resolve the KH23 and KH123 peaks. The unidentified ‘*’ impurity, which is at constant concentration during the reaction, provides a serendipitous control. Experiments were repeated three times. ( b ) (Left) Expected and measured masses of the reagents and product of the ligation. For the KH3 domain a 98% 15 N labelling has been assumed based on the level of enrichment of the 15 N nitrogen source. (Right) Reconstituted electrospray mass spectrum showing the de-convoluted molecular mass of the ligated KH123. ( c ) Superimposition of the backbone amide of 1 H{ 15 N} correlation experiments recorded on the KH3 and ligated KH3-only 15 N labelled KH123 proteins, indicating that the KH3 domain is correctly folded and assembled in the KH123 construct and highlighting the high quality of the NMR data. ( d ) The values of the 15 N longitudinal relaxation time of backbone amide groups in the ligated KH3 (black dots, this study) and of the isolated domains (orange dots, as previously reported 38 ) plotted along the protein sequence.
    Icontm Protein Concentrators, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/icontm protein concentrators/product/Thermo Fisher
    Average 77 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    icontm protein concentrators - by Bioz Stars, 2020-02
    77/100 stars
      Buy from Supplier

    97
    Bio-Rad protein concentrations
    <t>KH1-KH23</t> ligation. ( a ) HPLC traces showing the formation of the MPAA thioester intermediate and ligated KH123 product (identified by cartoons) over time. Diphenyl column was used to resolve the KH23 and KH123 peaks. The unidentified ‘*’ impurity, which is at constant concentration during the reaction, provides a serendipitous control. Experiments were repeated three times. ( b ) (Left) Expected and measured masses of the reagents and product of the ligation. For the KH3 domain a 98% 15 N labelling has been assumed based on the level of enrichment of the 15 N nitrogen source. (Right) Reconstituted electrospray mass spectrum showing the de-convoluted molecular mass of the ligated KH123. ( c ) Superimposition of the backbone amide of 1 H{ 15 N} correlation experiments recorded on the KH3 and ligated KH3-only 15 N labelled KH123 proteins, indicating that the KH3 domain is correctly folded and assembled in the KH123 construct and highlighting the high quality of the NMR data. ( d ) The values of the 15 N longitudinal relaxation time of backbone amide groups in the ligated KH3 (black dots, this study) and of the isolated domains (orange dots, as previously reported 38 ) plotted along the protein sequence.
    Protein Concentrations, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 4399 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protein concentrations/product/Bio-Rad
    Average 97 stars, based on 4399 article reviews
    Price from $9.99 to $1999.99
    protein concentrations - by Bioz Stars, 2020-02
    97/100 stars
      Buy from Supplier

    89
    Millipore amicon protein concentrator
    <t>KH1-KH23</t> ligation. ( a ) HPLC traces showing the formation of the MPAA thioester intermediate and ligated KH123 product (identified by cartoons) over time. Diphenyl column was used to resolve the KH23 and KH123 peaks. The unidentified ‘*’ impurity, which is at constant concentration during the reaction, provides a serendipitous control. Experiments were repeated three times. ( b ) (Left) Expected and measured masses of the reagents and product of the ligation. For the KH3 domain a 98% 15 N labelling has been assumed based on the level of enrichment of the 15 N nitrogen source. (Right) Reconstituted electrospray mass spectrum showing the de-convoluted molecular mass of the ligated KH123. ( c ) Superimposition of the backbone amide of 1 H{ 15 N} correlation experiments recorded on the KH3 and ligated KH3-only 15 N labelled KH123 proteins, indicating that the KH3 domain is correctly folded and assembled in the KH123 construct and highlighting the high quality of the NMR data. ( d ) The values of the 15 N longitudinal relaxation time of backbone amide groups in the ligated KH3 (black dots, this study) and of the isolated domains (orange dots, as previously reported 38 ) plotted along the protein sequence.
    Amicon Protein Concentrator, supplied by Millipore, used in various techniques. Bioz Stars score: 89/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amicon protein concentrator/product/Millipore
    Average 89 stars, based on 55 article reviews
    Price from $9.99 to $1999.99
    amicon protein concentrator - by Bioz Stars, 2020-02
    89/100 stars
      Buy from Supplier

    77
    Millipore ultrafree protein concentrator
    <t>KH1-KH23</t> ligation. ( a ) HPLC traces showing the formation of the MPAA thioester intermediate and ligated KH123 product (identified by cartoons) over time. Diphenyl column was used to resolve the KH23 and KH123 peaks. The unidentified ‘*’ impurity, which is at constant concentration during the reaction, provides a serendipitous control. Experiments were repeated three times. ( b ) (Left) Expected and measured masses of the reagents and product of the ligation. For the KH3 domain a 98% 15 N labelling has been assumed based on the level of enrichment of the 15 N nitrogen source. (Right) Reconstituted electrospray mass spectrum showing the de-convoluted molecular mass of the ligated KH123. ( c ) Superimposition of the backbone amide of 1 H{ 15 N} correlation experiments recorded on the KH3 and ligated KH3-only 15 N labelled KH123 proteins, indicating that the KH3 domain is correctly folded and assembled in the KH123 construct and highlighting the high quality of the NMR data. ( d ) The values of the 15 N longitudinal relaxation time of backbone amide groups in the ligated KH3 (black dots, this study) and of the isolated domains (orange dots, as previously reported 38 ) plotted along the protein sequence.
    Ultrafree Protein Concentrator, supplied by Millipore, used in various techniques. Bioz Stars score: 77/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ultrafree protein concentrator/product/Millipore
    Average 77 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    ultrafree protein concentrator - by Bioz Stars, 2020-02
    77/100 stars
      Buy from Supplier

    78
    Sartorius AG vivaspin6 protein concentrators
    <t>KH1-KH23</t> ligation. ( a ) HPLC traces showing the formation of the MPAA thioester intermediate and ligated KH123 product (identified by cartoons) over time. Diphenyl column was used to resolve the KH23 and KH123 peaks. The unidentified ‘*’ impurity, which is at constant concentration during the reaction, provides a serendipitous control. Experiments were repeated three times. ( b ) (Left) Expected and measured masses of the reagents and product of the ligation. For the KH3 domain a 98% 15 N labelling has been assumed based on the level of enrichment of the 15 N nitrogen source. (Right) Reconstituted electrospray mass spectrum showing the de-convoluted molecular mass of the ligated KH123. ( c ) Superimposition of the backbone amide of 1 H{ 15 N} correlation experiments recorded on the KH3 and ligated KH3-only 15 N labelled KH123 proteins, indicating that the KH3 domain is correctly folded and assembled in the KH123 construct and highlighting the high quality of the NMR data. ( d ) The values of the 15 N longitudinal relaxation time of backbone amide groups in the ligated KH3 (black dots, this study) and of the isolated domains (orange dots, as previously reported 38 ) plotted along the protein sequence.
    Vivaspin6 Protein Concentrators, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vivaspin6 protein concentrators/product/Sartorius AG
    Average 78 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    vivaspin6 protein concentrators - by Bioz Stars, 2020-02
    78/100 stars
      Buy from Supplier

    76
    Millipore centriplus protein concentrators
    <t>KH1-KH23</t> ligation. ( a ) HPLC traces showing the formation of the MPAA thioester intermediate and ligated KH123 product (identified by cartoons) over time. Diphenyl column was used to resolve the KH23 and KH123 peaks. The unidentified ‘*’ impurity, which is at constant concentration during the reaction, provides a serendipitous control. Experiments were repeated three times. ( b ) (Left) Expected and measured masses of the reagents and product of the ligation. For the KH3 domain a 98% 15 N labelling has been assumed based on the level of enrichment of the 15 N nitrogen source. (Right) Reconstituted electrospray mass spectrum showing the de-convoluted molecular mass of the ligated KH123. ( c ) Superimposition of the backbone amide of 1 H{ 15 N} correlation experiments recorded on the KH3 and ligated KH3-only 15 N labelled KH123 proteins, indicating that the KH3 domain is correctly folded and assembled in the KH123 construct and highlighting the high quality of the NMR data. ( d ) The values of the 15 N longitudinal relaxation time of backbone amide groups in the ligated KH3 (black dots, this study) and of the isolated domains (orange dots, as previously reported 38 ) plotted along the protein sequence.
    Centriplus Protein Concentrators, supplied by Millipore, used in various techniques. Bioz Stars score: 76/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/centriplus protein concentrators/product/Millipore
    Average 76 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    centriplus protein concentrators - by Bioz Stars, 2020-02
    76/100 stars
      Buy from Supplier

    80
    Sartorius AG vivaspin protein concentrator
    <t>KH1-KH23</t> ligation. ( a ) HPLC traces showing the formation of the MPAA thioester intermediate and ligated KH123 product (identified by cartoons) over time. Diphenyl column was used to resolve the KH23 and KH123 peaks. The unidentified ‘*’ impurity, which is at constant concentration during the reaction, provides a serendipitous control. Experiments were repeated three times. ( b ) (Left) Expected and measured masses of the reagents and product of the ligation. For the KH3 domain a 98% 15 N labelling has been assumed based on the level of enrichment of the 15 N nitrogen source. (Right) Reconstituted electrospray mass spectrum showing the de-convoluted molecular mass of the ligated KH123. ( c ) Superimposition of the backbone amide of 1 H{ 15 N} correlation experiments recorded on the KH3 and ligated KH3-only 15 N labelled KH123 proteins, indicating that the KH3 domain is correctly folded and assembled in the KH123 construct and highlighting the high quality of the NMR data. ( d ) The values of the 15 N longitudinal relaxation time of backbone amide groups in the ligated KH3 (black dots, this study) and of the isolated domains (orange dots, as previously reported 38 ) plotted along the protein sequence.
    Vivaspin Protein Concentrator, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 80/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vivaspin protein concentrator/product/Sartorius AG
    Average 80 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    vivaspin protein concentrator - by Bioz Stars, 2020-02
    80/100 stars
      Buy from Supplier

    90
    GE Healthcare vivaspin protein concentrators
    <t>KH1-KH23</t> ligation. ( a ) HPLC traces showing the formation of the MPAA thioester intermediate and ligated KH123 product (identified by cartoons) over time. Diphenyl column was used to resolve the KH23 and KH123 peaks. The unidentified ‘*’ impurity, which is at constant concentration during the reaction, provides a serendipitous control. Experiments were repeated three times. ( b ) (Left) Expected and measured masses of the reagents and product of the ligation. For the KH3 domain a 98% 15 N labelling has been assumed based on the level of enrichment of the 15 N nitrogen source. (Right) Reconstituted electrospray mass spectrum showing the de-convoluted molecular mass of the ligated KH123. ( c ) Superimposition of the backbone amide of 1 H{ 15 N} correlation experiments recorded on the KH3 and ligated KH3-only 15 N labelled KH123 proteins, indicating that the KH3 domain is correctly folded and assembled in the KH123 construct and highlighting the high quality of the NMR data. ( d ) The values of the 15 N longitudinal relaxation time of backbone amide groups in the ligated KH3 (black dots, this study) and of the isolated domains (orange dots, as previously reported 38 ) plotted along the protein sequence.
    Vivaspin Protein Concentrators, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 90/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vivaspin protein concentrators/product/GE Healthcare
    Average 90 stars, based on 25 article reviews
    Price from $9.99 to $1999.99
    vivaspin protein concentrators - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    77
    Corning Life Sciences 10k protein concentrator
    <t>KH1-KH23</t> ligation. ( a ) HPLC traces showing the formation of the MPAA thioester intermediate and ligated KH123 product (identified by cartoons) over time. Diphenyl column was used to resolve the KH23 and KH123 peaks. The unidentified ‘*’ impurity, which is at constant concentration during the reaction, provides a serendipitous control. Experiments were repeated three times. ( b ) (Left) Expected and measured masses of the reagents and product of the ligation. For the KH3 domain a 98% 15 N labelling has been assumed based on the level of enrichment of the 15 N nitrogen source. (Right) Reconstituted electrospray mass spectrum showing the de-convoluted molecular mass of the ligated KH123. ( c ) Superimposition of the backbone amide of 1 H{ 15 N} correlation experiments recorded on the KH3 and ligated KH3-only 15 N labelled KH123 proteins, indicating that the KH3 domain is correctly folded and assembled in the KH123 construct and highlighting the high quality of the NMR data. ( d ) The values of the 15 N longitudinal relaxation time of backbone amide groups in the ligated KH3 (black dots, this study) and of the isolated domains (orange dots, as previously reported 38 ) plotted along the protein sequence.
    10k Protein Concentrator, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 77/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/10k protein concentrator/product/Corning Life Sciences
    Average 77 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    10k protein concentrator - by Bioz Stars, 2020-02
    77/100 stars
      Buy from Supplier

    76
    Corning Life Sciences mwco protein concentrators
    <t>KH1-KH23</t> ligation. ( a ) HPLC traces showing the formation of the MPAA thioester intermediate and ligated KH123 product (identified by cartoons) over time. Diphenyl column was used to resolve the KH23 and KH123 peaks. The unidentified ‘*’ impurity, which is at constant concentration during the reaction, provides a serendipitous control. Experiments were repeated three times. ( b ) (Left) Expected and measured masses of the reagents and product of the ligation. For the KH3 domain a 98% 15 N labelling has been assumed based on the level of enrichment of the 15 N nitrogen source. (Right) Reconstituted electrospray mass spectrum showing the de-convoluted molecular mass of the ligated KH123. ( c ) Superimposition of the backbone amide of 1 H{ 15 N} correlation experiments recorded on the KH3 and ligated KH3-only 15 N labelled KH123 proteins, indicating that the KH3 domain is correctly folded and assembled in the KH123 construct and highlighting the high quality of the NMR data. ( d ) The values of the 15 N longitudinal relaxation time of backbone amide groups in the ligated KH3 (black dots, this study) and of the isolated domains (orange dots, as previously reported 38 ) plotted along the protein sequence.
    Mwco Protein Concentrators, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 76/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mwco protein concentrators/product/Corning Life Sciences
    Average 76 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    mwco protein concentrators - by Bioz Stars, 2020-02
    76/100 stars
      Buy from Supplier

    90
    Thermo Fisher pierce 9k mwco protein concentrators
    <t>KH1-KH23</t> ligation. ( a ) HPLC traces showing the formation of the MPAA thioester intermediate and ligated KH123 product (identified by cartoons) over time. Diphenyl column was used to resolve the KH23 and KH123 peaks. The unidentified ‘*’ impurity, which is at constant concentration during the reaction, provides a serendipitous control. Experiments were repeated three times. ( b ) (Left) Expected and measured masses of the reagents and product of the ligation. For the KH3 domain a 98% 15 N labelling has been assumed based on the level of enrichment of the 15 N nitrogen source. (Right) Reconstituted electrospray mass spectrum showing the de-convoluted molecular mass of the ligated KH123. ( c ) Superimposition of the backbone amide of 1 H{ 15 N} correlation experiments recorded on the KH3 and ligated KH3-only 15 N labelled KH123 proteins, indicating that the KH3 domain is correctly folded and assembled in the KH123 construct and highlighting the high quality of the NMR data. ( d ) The values of the 15 N longitudinal relaxation time of backbone amide groups in the ligated KH3 (black dots, this study) and of the isolated domains (orange dots, as previously reported 38 ) plotted along the protein sequence.
    Pierce 9k Mwco Protein Concentrators, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pierce 9k mwco protein concentrators/product/Thermo Fisher
    Average 90 stars, based on 66 article reviews
    Price from $9.99 to $1999.99
    pierce 9k mwco protein concentrators - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    86
    Thermo Fisher pierce protein concentrators pes 10k mwco
    <t>KH1-KH23</t> ligation. ( a ) HPLC traces showing the formation of the MPAA thioester intermediate and ligated KH123 product (identified by cartoons) over time. Diphenyl column was used to resolve the KH23 and KH123 peaks. The unidentified ‘*’ impurity, which is at constant concentration during the reaction, provides a serendipitous control. Experiments were repeated three times. ( b ) (Left) Expected and measured masses of the reagents and product of the ligation. For the KH3 domain a 98% 15 N labelling has been assumed based on the level of enrichment of the 15 N nitrogen source. (Right) Reconstituted electrospray mass spectrum showing the de-convoluted molecular mass of the ligated KH123. ( c ) Superimposition of the backbone amide of 1 H{ 15 N} correlation experiments recorded on the KH3 and ligated KH3-only 15 N labelled KH123 proteins, indicating that the KH3 domain is correctly folded and assembled in the KH123 construct and highlighting the high quality of the NMR data. ( d ) The values of the 15 N longitudinal relaxation time of backbone amide groups in the ligated KH3 (black dots, this study) and of the isolated domains (orange dots, as previously reported 38 ) plotted along the protein sequence.
    Pierce Protein Concentrators Pes 10k Mwco, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pierce protein concentrators pes 10k mwco/product/Thermo Fisher
    Average 86 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    pierce protein concentrators pes 10k mwco - by Bioz Stars, 2020-02
    86/100 stars
      Buy from Supplier

    85
    Thermo Fisher pierce 20k mwco protein concentrators
    <t>KH1-KH23</t> ligation. ( a ) HPLC traces showing the formation of the MPAA thioester intermediate and ligated KH123 product (identified by cartoons) over time. Diphenyl column was used to resolve the KH23 and KH123 peaks. The unidentified ‘*’ impurity, which is at constant concentration during the reaction, provides a serendipitous control. Experiments were repeated three times. ( b ) (Left) Expected and measured masses of the reagents and product of the ligation. For the KH3 domain a 98% 15 N labelling has been assumed based on the level of enrichment of the 15 N nitrogen source. (Right) Reconstituted electrospray mass spectrum showing the de-convoluted molecular mass of the ligated KH123. ( c ) Superimposition of the backbone amide of 1 H{ 15 N} correlation experiments recorded on the KH3 and ligated KH3-only 15 N labelled KH123 proteins, indicating that the KH3 domain is correctly folded and assembled in the KH123 construct and highlighting the high quality of the NMR data. ( d ) The values of the 15 N longitudinal relaxation time of backbone amide groups in the ligated KH3 (black dots, this study) and of the isolated domains (orange dots, as previously reported 38 ) plotted along the protein sequence.
    Pierce 20k Mwco Protein Concentrators, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pierce 20k mwco protein concentrators/product/Thermo Fisher
    Average 85 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    pierce 20k mwco protein concentrators - by Bioz Stars, 2020-02
    85/100 stars
      Buy from Supplier

    96
    Millipore amicon ultra protein concentrator
    <t>KH1-KH23</t> ligation. ( a ) HPLC traces showing the formation of the MPAA thioester intermediate and ligated KH123 product (identified by cartoons) over time. Diphenyl column was used to resolve the KH23 and KH123 peaks. The unidentified ‘*’ impurity, which is at constant concentration during the reaction, provides a serendipitous control. Experiments were repeated three times. ( b ) (Left) Expected and measured masses of the reagents and product of the ligation. For the KH3 domain a 98% 15 N labelling has been assumed based on the level of enrichment of the 15 N nitrogen source. (Right) Reconstituted electrospray mass spectrum showing the de-convoluted molecular mass of the ligated KH123. ( c ) Superimposition of the backbone amide of 1 H{ 15 N} correlation experiments recorded on the KH3 and ligated KH3-only 15 N labelled KH123 proteins, indicating that the KH3 domain is correctly folded and assembled in the KH123 construct and highlighting the high quality of the NMR data. ( d ) The values of the 15 N longitudinal relaxation time of backbone amide groups in the ligated KH3 (black dots, this study) and of the isolated domains (orange dots, as previously reported 38 ) plotted along the protein sequence.
    Amicon Ultra Protein Concentrator, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amicon ultra protein concentrator/product/Millipore
    Average 96 stars, based on 45 article reviews
    Price from $9.99 to $1999.99
    amicon ultra protein concentrator - by Bioz Stars, 2020-02
    96/100 stars
      Buy from Supplier

    79
    Thermo Fisher 9000 mwco protein concentrator
    <t>KH1-KH23</t> ligation. ( a ) HPLC traces showing the formation of the MPAA thioester intermediate and ligated KH123 product (identified by cartoons) over time. Diphenyl column was used to resolve the KH23 and KH123 peaks. The unidentified ‘*’ impurity, which is at constant concentration during the reaction, provides a serendipitous control. Experiments were repeated three times. ( b ) (Left) Expected and measured masses of the reagents and product of the ligation. For the KH3 domain a 98% 15 N labelling has been assumed based on the level of enrichment of the 15 N nitrogen source. (Right) Reconstituted electrospray mass spectrum showing the de-convoluted molecular mass of the ligated KH123. ( c ) Superimposition of the backbone amide of 1 H{ 15 N} correlation experiments recorded on the KH3 and ligated KH3-only 15 N labelled KH123 proteins, indicating that the KH3 domain is correctly folded and assembled in the KH123 construct and highlighting the high quality of the NMR data. ( d ) The values of the 15 N longitudinal relaxation time of backbone amide groups in the ligated KH3 (black dots, this study) and of the isolated domains (orange dots, as previously reported 38 ) plotted along the protein sequence.
    9000 Mwco Protein Concentrator, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/9000 mwco protein concentrator/product/Thermo Fisher
    Average 79 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    9000 mwco protein concentrator - by Bioz Stars, 2020-02
    79/100 stars
      Buy from Supplier

    88
    Thermo Fisher 150 kd protein concentrator
    SMPD1 expression levels correlate with total SMase content in supernatant in MM cell lines and their exosomes. ( A ) Basal-dCT values of all four genes coding for SMases (SMPD1–4) measured by qRT-PCR in four human MM cell lines (JJN3, OPM2, LP1, and U266) after 24 h of culture. The housekeeping gene ABL1 was used for data normalization, and differential gene expression was determined using the comparative ΔΔCt method. ( B ) Basal total SMase content determined in supernatans by an AmplexRed SMase assay after 24 h of culture for all four cell lines. The amount of SMase activity measured in these cell lines corresponded best to SMPD1 expression, coding for acid SMase (ASM). ( C ) Cells and exosomes were isolated after 24 h of culture and analyzed for ASM content and exosomal markers, CD81 and CD63. Nanoparticle tracking analysis by Zetaview analysis shows a mean diameter of <t>50–150</t> nm, confirming the expected size of exosomes. Immunoblot can be found in Supplementary Figure S4B . ASM = acid sphingomyelinase, exo = exosomes. In A and B, the averages of n > 3 are shown.
    150 Kd Protein Concentrator, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/150 kd protein concentrator/product/Thermo Fisher
    Average 88 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    150 kd protein concentrator - by Bioz Stars, 2020-02
    88/100 stars
      Buy from Supplier

    79
    Fisher Scientific vivaspin 20 protein concentrator
    SMPD1 expression levels correlate with total SMase content in supernatant in MM cell lines and their exosomes. ( A ) Basal-dCT values of all four genes coding for SMases (SMPD1–4) measured by qRT-PCR in four human MM cell lines (JJN3, OPM2, LP1, and U266) after 24 h of culture. The housekeeping gene ABL1 was used for data normalization, and differential gene expression was determined using the comparative ΔΔCt method. ( B ) Basal total SMase content determined in supernatans by an AmplexRed SMase assay after 24 h of culture for all four cell lines. The amount of SMase activity measured in these cell lines corresponded best to SMPD1 expression, coding for acid SMase (ASM). ( C ) Cells and exosomes were isolated after 24 h of culture and analyzed for ASM content and exosomal markers, CD81 and CD63. Nanoparticle tracking analysis by Zetaview analysis shows a mean diameter of <t>50–150</t> nm, confirming the expected size of exosomes. Immunoblot can be found in Supplementary Figure S4B . ASM = acid sphingomyelinase, exo = exosomes. In A and B, the averages of n > 3 are shown.
    Vivaspin 20 Protein Concentrator, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 79/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vivaspin 20 protein concentrator/product/Fisher Scientific
    Average 79 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    vivaspin 20 protein concentrator - by Bioz Stars, 2020-02
    79/100 stars
      Buy from Supplier

    79
    Bio-Rad proteins concentration
    SMPD1 expression levels correlate with total SMase content in supernatant in MM cell lines and their exosomes. ( A ) Basal-dCT values of all four genes coding for SMases (SMPD1–4) measured by qRT-PCR in four human MM cell lines (JJN3, OPM2, LP1, and U266) after 24 h of culture. The housekeeping gene ABL1 was used for data normalization, and differential gene expression was determined using the comparative ΔΔCt method. ( B ) Basal total SMase content determined in supernatans by an AmplexRed SMase assay after 24 h of culture for all four cell lines. The amount of SMase activity measured in these cell lines corresponded best to SMPD1 expression, coding for acid SMase (ASM). ( C ) Cells and exosomes were isolated after 24 h of culture and analyzed for ASM content and exosomal markers, CD81 and CD63. Nanoparticle tracking analysis by Zetaview analysis shows a mean diameter of <t>50–150</t> nm, confirming the expected size of exosomes. Immunoblot can be found in Supplementary Figure S4B . ASM = acid sphingomyelinase, exo = exosomes. In A and B, the averages of n > 3 are shown.
    Proteins Concentration, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/proteins concentration/product/Bio-Rad
    Average 79 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    proteins concentration - by Bioz Stars, 2020-02
    79/100 stars
      Buy from Supplier

    82
    GE Healthcare kd mwco protein concentrator
    SMPD1 expression levels correlate with total SMase content in supernatant in MM cell lines and their exosomes. ( A ) Basal-dCT values of all four genes coding for SMases (SMPD1–4) measured by qRT-PCR in four human MM cell lines (JJN3, OPM2, LP1, and U266) after 24 h of culture. The housekeeping gene ABL1 was used for data normalization, and differential gene expression was determined using the comparative ΔΔCt method. ( B ) Basal total SMase content determined in supernatans by an AmplexRed SMase assay after 24 h of culture for all four cell lines. The amount of SMase activity measured in these cell lines corresponded best to SMPD1 expression, coding for acid SMase (ASM). ( C ) Cells and exosomes were isolated after 24 h of culture and analyzed for ASM content and exosomal markers, CD81 and CD63. Nanoparticle tracking analysis by Zetaview analysis shows a mean diameter of <t>50–150</t> nm, confirming the expected size of exosomes. Immunoblot can be found in Supplementary Figure S4B . ASM = acid sphingomyelinase, exo = exosomes. In A and B, the averages of n > 3 are shown.
    Kd Mwco Protein Concentrator, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 82/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kd mwco protein concentrator/product/GE Healthcare
    Average 82 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    kd mwco protein concentrator - by Bioz Stars, 2020-02
    82/100 stars
      Buy from Supplier

    84
    Sartorius AG vivaspin 20 protein concentrator
    SMPD1 expression levels correlate with total SMase content in supernatant in MM cell lines and their exosomes. ( A ) Basal-dCT values of all four genes coding for SMases (SMPD1–4) measured by qRT-PCR in four human MM cell lines (JJN3, OPM2, LP1, and U266) after 24 h of culture. The housekeeping gene ABL1 was used for data normalization, and differential gene expression was determined using the comparative ΔΔCt method. ( B ) Basal total SMase content determined in supernatans by an AmplexRed SMase assay after 24 h of culture for all four cell lines. The amount of SMase activity measured in these cell lines corresponded best to SMPD1 expression, coding for acid SMase (ASM). ( C ) Cells and exosomes were isolated after 24 h of culture and analyzed for ASM content and exosomal markers, CD81 and CD63. Nanoparticle tracking analysis by Zetaview analysis shows a mean diameter of <t>50–150</t> nm, confirming the expected size of exosomes. Immunoblot can be found in Supplementary Figure S4B . ASM = acid sphingomyelinase, exo = exosomes. In A and B, the averages of n > 3 are shown.
    Vivaspin 20 Protein Concentrator, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 84/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vivaspin 20 protein concentrator/product/Sartorius AG
    Average 84 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    vivaspin 20 protein concentrator - by Bioz Stars, 2020-02
    84/100 stars
      Buy from Supplier

    78
    Prolactal Gmbh whey protein concentrate
    SMPD1 expression levels correlate with total SMase content in supernatant in MM cell lines and their exosomes. ( A ) Basal-dCT values of all four genes coding for SMases (SMPD1–4) measured by qRT-PCR in four human MM cell lines (JJN3, OPM2, LP1, and U266) after 24 h of culture. The housekeeping gene ABL1 was used for data normalization, and differential gene expression was determined using the comparative ΔΔCt method. ( B ) Basal total SMase content determined in supernatans by an AmplexRed SMase assay after 24 h of culture for all four cell lines. The amount of SMase activity measured in these cell lines corresponded best to SMPD1 expression, coding for acid SMase (ASM). ( C ) Cells and exosomes were isolated after 24 h of culture and analyzed for ASM content and exosomal markers, CD81 and CD63. Nanoparticle tracking analysis by Zetaview analysis shows a mean diameter of <t>50–150</t> nm, confirming the expected size of exosomes. Immunoblot can be found in Supplementary Figure S4B . ASM = acid sphingomyelinase, exo = exosomes. In A and B, the averages of n > 3 are shown.
    Whey Protein Concentrate, supplied by Prolactal Gmbh, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/whey protein concentrate/product/Prolactal Gmbh
    Average 78 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    whey protein concentrate - by Bioz Stars, 2020-02
    78/100 stars
      Buy from Supplier

    Image Search Results


    KH1-KH23 ligation. ( a ) HPLC traces showing the formation of the MPAA thioester intermediate and ligated KH123 product (identified by cartoons) over time. Diphenyl column was used to resolve the KH23 and KH123 peaks. The unidentified ‘*’ impurity, which is at constant concentration during the reaction, provides a serendipitous control. Experiments were repeated three times. ( b ) (Left) Expected and measured masses of the reagents and product of the ligation. For the KH3 domain a 98% 15 N labelling has been assumed based on the level of enrichment of the 15 N nitrogen source. (Right) Reconstituted electrospray mass spectrum showing the de-convoluted molecular mass of the ligated KH123. ( c ) Superimposition of the backbone amide of 1 H{ 15 N} correlation experiments recorded on the KH3 and ligated KH3-only 15 N labelled KH123 proteins, indicating that the KH3 domain is correctly folded and assembled in the KH123 construct and highlighting the high quality of the NMR data. ( d ) The values of the 15 N longitudinal relaxation time of backbone amide groups in the ligated KH3 (black dots, this study) and of the isolated domains (orange dots, as previously reported 38 ) plotted along the protein sequence.

    Journal: Scientific Reports

    Article Title: A method for the unbiased and efficient segmental labelling of RNA-binding proteins for structure and biophysics

    doi: 10.1038/s41598-017-13950-8

    Figure Lengend Snippet: KH1-KH23 ligation. ( a ) HPLC traces showing the formation of the MPAA thioester intermediate and ligated KH123 product (identified by cartoons) over time. Diphenyl column was used to resolve the KH23 and KH123 peaks. The unidentified ‘*’ impurity, which is at constant concentration during the reaction, provides a serendipitous control. Experiments were repeated three times. ( b ) (Left) Expected and measured masses of the reagents and product of the ligation. For the KH3 domain a 98% 15 N labelling has been assumed based on the level of enrichment of the 15 N nitrogen source. (Right) Reconstituted electrospray mass spectrum showing the de-convoluted molecular mass of the ligated KH123. ( c ) Superimposition of the backbone amide of 1 H{ 15 N} correlation experiments recorded on the KH3 and ligated KH3-only 15 N labelled KH123 proteins, indicating that the KH3 domain is correctly folded and assembled in the KH123 construct and highlighting the high quality of the NMR data. ( d ) The values of the 15 N longitudinal relaxation time of backbone amide groups in the ligated KH3 (black dots, this study) and of the isolated domains (orange dots, as previously reported 38 ) plotted along the protein sequence.

    Article Snippet: Protein expression Recombinant KH1 and KH2 fusion proteins were expressed in E. coli BL21(DE3) strain (Novagen, cat. no. 69450) growing in 100 µg/ml ampicillin LB media.

    Techniques: Ligation, High Performance Liquid Chromatography, Concentration Assay, Construct, Nuclear Magnetic Resonance, Isolation, Sequencing

    Workflow of the expressed protein ligation protocol for the KH1, KH2, and KH3 RNA binding domains of the RNA regulator protein KSRP. The domains are expressed as either intein-CBD or SUMO-His fusion proteins and purified by chitin and nickel agarose affinity resins respectively. Intein fusion proteins are released from the chitin affinity resins by the strongly nucleophilic thiol additive benzyl mercaptan, which results in benzyl thioester formation at the C -terminus of the released protein. Instead, proteolytic cleavage catalysed by either SUMO or TEV proteases form N -terminal cysteine species. The ligations are performed at 40 °C in 6 M guanidine, pH 6.5 using TCEP as reducing agent and MPAA as a catalyst.

    Journal: Scientific Reports

    Article Title: A method for the unbiased and efficient segmental labelling of RNA-binding proteins for structure and biophysics

    doi: 10.1038/s41598-017-13950-8

    Figure Lengend Snippet: Workflow of the expressed protein ligation protocol for the KH1, KH2, and KH3 RNA binding domains of the RNA regulator protein KSRP. The domains are expressed as either intein-CBD or SUMO-His fusion proteins and purified by chitin and nickel agarose affinity resins respectively. Intein fusion proteins are released from the chitin affinity resins by the strongly nucleophilic thiol additive benzyl mercaptan, which results in benzyl thioester formation at the C -terminus of the released protein. Instead, proteolytic cleavage catalysed by either SUMO or TEV proteases form N -terminal cysteine species. The ligations are performed at 40 °C in 6 M guanidine, pH 6.5 using TCEP as reducing agent and MPAA as a catalyst.

    Article Snippet: Protein expression Recombinant KH1 and KH2 fusion proteins were expressed in E. coli BL21(DE3) strain (Novagen, cat. no. 69450) growing in 100 µg/ml ampicillin LB media.

    Techniques: Ligation, RNA Binding Assay, Purification

    SMPD1 expression levels correlate with total SMase content in supernatant in MM cell lines and their exosomes. ( A ) Basal-dCT values of all four genes coding for SMases (SMPD1–4) measured by qRT-PCR in four human MM cell lines (JJN3, OPM2, LP1, and U266) after 24 h of culture. The housekeeping gene ABL1 was used for data normalization, and differential gene expression was determined using the comparative ΔΔCt method. ( B ) Basal total SMase content determined in supernatans by an AmplexRed SMase assay after 24 h of culture for all four cell lines. The amount of SMase activity measured in these cell lines corresponded best to SMPD1 expression, coding for acid SMase (ASM). ( C ) Cells and exosomes were isolated after 24 h of culture and analyzed for ASM content and exosomal markers, CD81 and CD63. Nanoparticle tracking analysis by Zetaview analysis shows a mean diameter of 50–150 nm, confirming the expected size of exosomes. Immunoblot can be found in Supplementary Figure S4B . ASM = acid sphingomyelinase, exo = exosomes. In A and B, the averages of n > 3 are shown.

    Journal: Cancers

    Article Title: The Transfer of Sphingomyelinase Contributes to Drug Resistance in Multiple Myeloma

    doi: 10.3390/cancers11121823

    Figure Lengend Snippet: SMPD1 expression levels correlate with total SMase content in supernatant in MM cell lines and their exosomes. ( A ) Basal-dCT values of all four genes coding for SMases (SMPD1–4) measured by qRT-PCR in four human MM cell lines (JJN3, OPM2, LP1, and U266) after 24 h of culture. The housekeeping gene ABL1 was used for data normalization, and differential gene expression was determined using the comparative ΔΔCt method. ( B ) Basal total SMase content determined in supernatans by an AmplexRed SMase assay after 24 h of culture for all four cell lines. The amount of SMase activity measured in these cell lines corresponded best to SMPD1 expression, coding for acid SMase (ASM). ( C ) Cells and exosomes were isolated after 24 h of culture and analyzed for ASM content and exosomal markers, CD81 and CD63. Nanoparticle tracking analysis by Zetaview analysis shows a mean diameter of 50–150 nm, confirming the expected size of exosomes. Immunoblot can be found in Supplementary Figure S4B . ASM = acid sphingomyelinase, exo = exosomes. In A and B, the averages of n > 3 are shown.

    Article Snippet: The filtered medium was concentrated using a 150 kD Protein Concentrator (Thermo Scientific, Waltham, MA, USA) and filtered again with a 0.22 µM pore filter.

    Techniques: Expressing, Quantitative RT-PCR, Activity Assay, Isolation