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    Bangs Laboratories concanavalin a coated magnetic beads
    An automated platform for high-throughput in situ profiling of chromatin proteins.  a  AutoCUT  RUN workflow. (1) Cells or tissue are bound to concanavalin A-coated beads, permeabilized with digitonin, and incubated with an antibody targeting a chromatin protein. (2) Samples are arrayed in a 96-well plate and (3) processed on a Biomek robot fitted with a 96-well magnetic plate for magnetic separation during washes (α), and an aluminum chiller block (β) routed to a circulating water bath (γ) for temperature control. (4) AutoCUT  RUN produces in 2 days up to 96 libraries that are ready to be pooled and sequenced.  b  Hierarchically clustered correlation matrix of AutoCUT  RUN profiles of histone-H3 modifications that mark active (pink) and repressed (blue) chromatin in H1 (orange) and K562 (purple) cells. Pearson correlations were calculated using the log 2 -transformed values of read counts split into 500 bp bins across the genome
    Concanavalin A Coated Magnetic Beads, supplied by Bangs Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/concanavalin a coated magnetic beads/product/Bangs Laboratories
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    concanavalin a coated magnetic beads - by Bioz Stars, 2021-04
    86/100 stars
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    99
    Millipore concanavalin a coated magnetic beads
    An automated platform for high-throughput in situ profiling of chromatin proteins.  a  AutoCUT  RUN workflow. (1) Cells or tissue are bound to concanavalin A-coated beads, permeabilized with digitonin, and incubated with an antibody targeting a chromatin protein. (2) Samples are arrayed in a 96-well plate and (3) processed on a Biomek robot fitted with a 96-well magnetic plate for magnetic separation during washes (α), and an aluminum chiller block (β) routed to a circulating water bath (γ) for temperature control. (4) AutoCUT  RUN produces in 2 days up to 96 libraries that are ready to be pooled and sequenced.  b  Hierarchically clustered correlation matrix of AutoCUT  RUN profiles of histone-H3 modifications that mark active (pink) and repressed (blue) chromatin in H1 (orange) and K562 (purple) cells. Pearson correlations were calculated using the log 2 -transformed values of read counts split into 500 bp bins across the genome
    Concanavalin A Coated Magnetic Beads, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/concanavalin a coated magnetic beads/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    concanavalin a coated magnetic beads - by Bioz Stars, 2021-04
    99/100 stars
      Buy from Supplier

    86
    Polysciences inc concanavalin a coated magnetic beads
    An automated platform for high-throughput in situ profiling of chromatin proteins.  a  AutoCUT  RUN workflow. (1) Cells or tissue are bound to concanavalin A-coated beads, permeabilized with digitonin, and incubated with an antibody targeting a chromatin protein. (2) Samples are arrayed in a 96-well plate and (3) processed on a Biomek robot fitted with a 96-well magnetic plate for magnetic separation during washes (α), and an aluminum chiller block (β) routed to a circulating water bath (γ) for temperature control. (4) AutoCUT  RUN produces in 2 days up to 96 libraries that are ready to be pooled and sequenced.  b  Hierarchically clustered correlation matrix of AutoCUT  RUN profiles of histone-H3 modifications that mark active (pink) and repressed (blue) chromatin in H1 (orange) and K562 (purple) cells. Pearson correlations were calculated using the log 2 -transformed values of read counts split into 500 bp bins across the genome
    Concanavalin A Coated Magnetic Beads, supplied by Polysciences inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/concanavalin a coated magnetic beads/product/Polysciences inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    concanavalin a coated magnetic beads - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    86
    Bangs Laboratories biomag plus concanavalin a coated magnetic beads
    An automated platform for high-throughput in situ profiling of chromatin proteins.  a  AutoCUT  RUN workflow. (1) Cells or tissue are bound to concanavalin A-coated beads, permeabilized with digitonin, and incubated with an antibody targeting a chromatin protein. (2) Samples are arrayed in a 96-well plate and (3) processed on a Biomek robot fitted with a 96-well magnetic plate for magnetic separation during washes (α), and an aluminum chiller block (β) routed to a circulating water bath (γ) for temperature control. (4) AutoCUT  RUN produces in 2 days up to 96 libraries that are ready to be pooled and sequenced.  b  Hierarchically clustered correlation matrix of AutoCUT  RUN profiles of histone-H3 modifications that mark active (pink) and repressed (blue) chromatin in H1 (orange) and K562 (purple) cells. Pearson correlations were calculated using the log 2 -transformed values of read counts split into 500 bp bins across the genome
    Biomag Plus Concanavalin A Coated Magnetic Beads, supplied by Bangs Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biomag plus concanavalin a coated magnetic beads/product/Bangs Laboratories
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biomag plus concanavalin a coated magnetic beads - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

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    An automated platform for high-throughput in situ profiling of chromatin proteins.  a  AutoCUT  RUN workflow. (1) Cells or tissue are bound to concanavalin A-coated beads, permeabilized with digitonin, and incubated with an antibody targeting a chromatin protein. (2) Samples are arrayed in a 96-well plate and (3) processed on a Biomek robot fitted with a 96-well magnetic plate for magnetic separation during washes (α), and an aluminum chiller block (β) routed to a circulating water bath (γ) for temperature control. (4) AutoCUT  RUN produces in 2 days up to 96 libraries that are ready to be pooled and sequenced.  b  Hierarchically clustered correlation matrix of AutoCUT  RUN profiles of histone-H3 modifications that mark active (pink) and repressed (blue) chromatin in H1 (orange) and K562 (purple) cells. Pearson correlations were calculated using the log 2 -transformed values of read counts split into 500 bp bins across the genome

    Journal: Epigenetics & Chromatin

    Article Title: Automated in situ chromatin profiling efficiently resolves cell types and gene regulatory programs

    doi: 10.1186/s13072-018-0243-8

    Figure Lengend Snippet: An automated platform for high-throughput in situ profiling of chromatin proteins. a AutoCUT RUN workflow. (1) Cells or tissue are bound to concanavalin A-coated beads, permeabilized with digitonin, and incubated with an antibody targeting a chromatin protein. (2) Samples are arrayed in a 96-well plate and (3) processed on a Biomek robot fitted with a 96-well magnetic plate for magnetic separation during washes (α), and an aluminum chiller block (β) routed to a circulating water bath (γ) for temperature control. (4) AutoCUT RUN produces in 2 days up to 96 libraries that are ready to be pooled and sequenced. b Hierarchically clustered correlation matrix of AutoCUT RUN profiles of histone-H3 modifications that mark active (pink) and repressed (blue) chromatin in H1 (orange) and K562 (purple) cells. Pearson correlations were calculated using the log 2 -transformed values of read counts split into 500 bp bins across the genome

    Article Snippet: Briefly, cells or tissue samples are bound to concanavalin A-coated magnetic beads (Bangs Laboratories, ca. no. BP531), permeabilized with digitonin, and bound with a protein specific antibody as previously described [ ].

    Techniques: High Throughput Screening Assay, In Situ, Incubation, Blocking Assay, Transformation Assay