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    Thermo Fisher competent e coli thermo fisher c404006 chemicals
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    Thermo Fisher one shot top10 escherichia coli
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    Thermo Fisher competent e coli top10
    Overexpression and purification of the 45-kDa recombinant GDH protein. The protein was overexpressed and purified as described in Materials and Methods. An SDS-10% polyacrylamide gel stained with Coomassie brilliant blue R-250 is shown. Lanes: M, rainbow molecular size marker in kilodaltons; 1, whole-cell lysate of pOT411 transformant of E. coli <t>TOP10</t> uninduced; 2, whole-cell lysate of E. coli transformed with pOT411 and induced with arabinose; 3 and 4, different amounts of the recombinant protein purified from pOT411 transformant of E. coli TOP10.
    Competent E Coli Top10, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 431 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/competent e coli top10/product/Thermo Fisher
    Average 99 stars, based on 431 article reviews
    Price from $9.99 to $1999.99
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    Overexpression and purification of the 45-kDa recombinant GDH protein. The protein was overexpressed and purified as described in Materials and Methods. An SDS-10% polyacrylamide gel stained with Coomassie brilliant blue R-250 is shown. Lanes: M, rainbow molecular size marker in kilodaltons; 1, whole-cell lysate of pOT411 transformant of E. coli TOP10 uninduced; 2, whole-cell lysate of E. coli transformed with pOT411 and induced with arabinose; 3 and 4, different amounts of the recombinant protein purified from pOT411 transformant of E. coli TOP10.

    Journal: Clinical and Diagnostic Laboratory Immunology

    Article Title: Cloning and Characterization of the Gene Encoding the Glutamate Dehydrogenase of Streptococcus suis Serotype 2

    doi: 10.1128/CDLI.8.2.251-257.2001

    Figure Lengend Snippet: Overexpression and purification of the 45-kDa recombinant GDH protein. The protein was overexpressed and purified as described in Materials and Methods. An SDS-10% polyacrylamide gel stained with Coomassie brilliant blue R-250 is shown. Lanes: M, rainbow molecular size marker in kilodaltons; 1, whole-cell lysate of pOT411 transformant of E. coli TOP10 uninduced; 2, whole-cell lysate of E. coli transformed with pOT411 and induced with arabinose; 3 and 4, different amounts of the recombinant protein purified from pOT411 transformant of E. coli TOP10.

    Article Snippet: The DNA insert in pOT410 was cloned in frame into a pBAD/ Myc -His version B expression vector to create pOT411. pOT411 was transformed into E. coli TOP10-competent cells and overexpressed by following the manufacturer's protocol (Invitrogen).

    Techniques: Over Expression, Purification, Recombinant, Staining, Marker, Transformation Assay