competent e.coli Search Results


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  • 99
    New England Biolabs competent e coli
    Competent E Coli, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 461 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC competent e coli mg1655
    Competent E Coli Mg1655, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC competent e coli k802
    Competent E Coli K802, supplied by ATCC, used in various techniques. Bioz Stars score: 85/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore competent e coli
    Competent E Coli, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 214 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad competent e coli
    Competent E Coli, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Meridian Life Science competent e coli
    Competent E Coli, supplied by Meridian Life Science, used in various techniques. Bioz Stars score: 92/100, based on 87 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher competent e coli
    A; Diagram of the construction of the entry and expression clones. The HepG2 cell line was used as a source of FVII cDNA. Blunt-end PCR product was TOPO-cloned into the pENTR TOPO/D vector. The construct was used to transform competent  E. coli  and positive clones were selected on LB medium containing an appropriate antibiotic. This construct is called the entry clone. Next, LR recombinant reaction was carried out between pENTR TOPO/D-FVII and pDEST26 vector to construct the expression clone. The resulting pDEST26-FVII, which is called the expression clone, was transfected into CHO cells. Stable clones expressing recombinant FVII were established in the presence of geneticin. B; pDEST26 vector. C; N-terminal sequence of the fusion protein.
    Competent E Coli, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5958 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TransGen biotech co competent e coli
    A; Diagram of the construction of the entry and expression clones. The HepG2 cell line was used as a source of FVII cDNA. Blunt-end PCR product was TOPO-cloned into the pENTR TOPO/D vector. The construct was used to transform competent  E. coli  and positive clones were selected on LB medium containing an appropriate antibiotic. This construct is called the entry clone. Next, LR recombinant reaction was carried out between pENTR TOPO/D-FVII and pDEST26 vector to construct the expression clone. The resulting pDEST26-FVII, which is called the expression clone, was transfected into CHO cells. Stable clones expressing recombinant FVII were established in the presence of geneticin. B; pDEST26 vector. C; N-terminal sequence of the fusion protein.
    Competent E Coli, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene competent e coli
    A; Diagram of the construction of the entry and expression clones. The HepG2 cell line was used as a source of FVII cDNA. Blunt-end PCR product was TOPO-cloned into the pENTR TOPO/D vector. The construct was used to transform competent  E. coli  and positive clones were selected on LB medium containing an appropriate antibiotic. This construct is called the entry clone. Next, LR recombinant reaction was carried out between pENTR TOPO/D-FVII and pDEST26 vector to construct the expression clone. The resulting pDEST26-FVII, which is called the expression clone, was transfected into CHO cells. Stable clones expressing recombinant FVII were established in the presence of geneticin. B; pDEST26 vector. C; N-terminal sequence of the fusion protein.
    Competent E Coli, supplied by Stratagene, used in various techniques. Bioz Stars score: 93/100, based on 224 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Avantor competent escherichia coli
    A; Diagram of the construction of the entry and expression clones. The HepG2 cell line was used as a source of FVII cDNA. Blunt-end PCR product was TOPO-cloned into the pENTR TOPO/D vector. The construct was used to transform competent  E. coli  and positive clones were selected on LB medium containing an appropriate antibiotic. This construct is called the entry clone. Next, LR recombinant reaction was carried out between pENTR TOPO/D-FVII and pDEST26 vector to construct the expression clone. The resulting pDEST26-FVII, which is called the expression clone, was transfected into CHO cells. Stable clones expressing recombinant FVII were established in the presence of geneticin. B; pDEST26 vector. C; N-terminal sequence of the fusion protein.
    Competent Escherichia Coli, supplied by Avantor, used in various techniques. Bioz Stars score: 91/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs bl21 competent e coli
    Examples of the differences between the original RefSeq annotation and our reannotation. a In the reannotation, one pseudogene (RS16270) was identified as two genes, ins A and ins B, which show strong homology to the insertion element protein, IS1. b In the reannotation, two pseudogenes were re-identified as two genes ( lac Z1 and lac Z2), whereas the hypothetical protein was reannotated and shown to be highly homologous with the DNA-directed RNA polymerase gene ECBD_2906 from E. coli strain <t>BL21-DE3</t>
    Bl21 Competent E Coli, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 157 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore competent e coli rosetta
    Examples of the differences between the original RefSeq annotation and our reannotation. a In the reannotation, one pseudogene (RS16270) was identified as two genes, ins A and ins B, which show strong homology to the insertion element protein, IS1. b In the reannotation, two pseudogenes were re-identified as two genes ( lac Z1 and lac Z2), whereas the hypothetical protein was reannotated and shown to be highly homologous with the DNA-directed RNA polymerase gene ECBD_2906 from E. coli strain <t>BL21-DE3</t>
    Competent E Coli Rosetta, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher dh10b competent e coli
    Examples of the differences between the original RefSeq annotation and our reannotation. a In the reannotation, one pseudogene (RS16270) was identified as two genes, ins A and ins B, which show strong homology to the insertion element protein, IS1. b In the reannotation, two pseudogenes were re-identified as two genes ( lac Z1 and lac Z2), whereas the hypothetical protein was reannotated and shown to be highly homologous with the DNA-directed RNA polymerase gene ECBD_2906 from E. coli strain <t>BL21-DE3</t>
    Dh10b Competent E Coli, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher competent e coli dh5α
    Examples of the differences between the original RefSeq annotation and our reannotation. a In the reannotation, one pseudogene (RS16270) was identified as two genes, ins A and ins B, which show strong homology to the insertion element protein, IS1. b In the reannotation, two pseudogenes were re-identified as two genes ( lac Z1 and lac Z2), whereas the hypothetical protein was reannotated and shown to be highly homologous with the DNA-directed RNA polymerase gene ECBD_2906 from E. coli strain <t>BL21-DE3</t>
    Competent E Coli Dh5α, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 442 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega dh5α competent e coli
    Examples of the differences between the original RefSeq annotation and our reannotation. a In the reannotation, one pseudogene (RS16270) was identified as two genes, ins A and ins B, which show strong homology to the insertion element protein, IS1. b In the reannotation, two pseudogenes were re-identified as two genes ( lac Z1 and lac Z2), whereas the hypothetical protein was reannotated and shown to be highly homologous with the DNA-directed RNA polymerase gene ECBD_2906 from E. coli strain <t>BL21-DE3</t>
    Dh5α Competent E Coli, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    TaKaRa competent e coli dh5α
    Examples of the differences between the original RefSeq annotation and our reannotation. a In the reannotation, one pseudogene (RS16270) was identified as two genes, ins A and ins B, which show strong homology to the insertion element protein, IS1. b In the reannotation, two pseudogenes were re-identified as two genes ( lac Z1 and lac Z2), whereas the hypothetical protein was reannotated and shown to be highly homologous with the DNA-directed RNA polymerase gene ECBD_2906 from E. coli strain <t>BL21-DE3</t>
    Competent E Coli Dh5α, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 85 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Thermo Fisher dh10bac competent e coli
    Examples of the differences between the original RefSeq annotation and our reannotation. a In the reannotation, one pseudogene (RS16270) was identified as two genes, ins A and ins B, which show strong homology to the insertion element protein, IS1. b In the reannotation, two pseudogenes were re-identified as two genes ( lac Z1 and lac Z2), whereas the hypothetical protein was reannotated and shown to be highly homologous with the DNA-directed RNA polymerase gene ECBD_2906 from E. coli strain <t>BL21-DE3</t>
    Dh10bac Competent E Coli, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Genlantis solubl21 competent escherichia coli
    Examples of the differences between the original RefSeq annotation and our reannotation. a In the reannotation, one pseudogene (RS16270) was identified as two genes, ins A and ins B, which show strong homology to the insertion element protein, IS1. b In the reannotation, two pseudogenes were re-identified as two genes ( lac Z1 and lac Z2), whereas the hypothetical protein was reannotated and shown to be highly homologous with the DNA-directed RNA polymerase gene ECBD_2906 from E. coli strain <t>BL21-DE3</t>
    Solubl21 Competent Escherichia Coli, supplied by Genlantis, used in various techniques. Bioz Stars score: 90/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore competent e coli origami
    Examples of the differences between the original RefSeq annotation and our reannotation. a In the reannotation, one pseudogene (RS16270) was identified as two genes, ins A and ins B, which show strong homology to the insertion element protein, IS1. b In the reannotation, two pseudogenes were re-identified as two genes ( lac Z1 and lac Z2), whereas the hypothetical protein was reannotated and shown to be highly homologous with the DNA-directed RNA polymerase gene ECBD_2906 from E. coli strain <t>BL21-DE3</t>
    Competent E Coli Origami, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher competent e coli mc1061
    Examples of the differences between the original RefSeq annotation and our reannotation. a In the reannotation, one pseudogene (RS16270) was identified as two genes, ins A and ins B, which show strong homology to the insertion element protein, IS1. b In the reannotation, two pseudogenes were re-identified as two genes ( lac Z1 and lac Z2), whereas the hypothetical protein was reannotated and shown to be highly homologous with the DNA-directed RNA polymerase gene ECBD_2906 from E. coli strain <t>BL21-DE3</t>
    Competent E Coli Mc1061, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher invαf escherichia coli
    Examples of the differences between the original RefSeq annotation and our reannotation. a In the reannotation, one pseudogene (RS16270) was identified as two genes, ins A and ins B, which show strong homology to the insertion element protein, IS1. b In the reannotation, two pseudogenes were re-identified as two genes ( lac Z1 and lac Z2), whereas the hypothetical protein was reannotated and shown to be highly homologous with the DNA-directed RNA polymerase gene ECBD_2906 from E. coli strain <t>BL21-DE3</t>
    Invαf Escherichia Coli, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs bl21 t7
    Examples of the differences between the original RefSeq annotation and our reannotation. a In the reannotation, one pseudogene (RS16270) was identified as two genes, ins A and ins B, which show strong homology to the insertion element protein, IS1. b In the reannotation, two pseudogenes were re-identified as two genes ( lac Z1 and lac Z2), whereas the hypothetical protein was reannotated and shown to be highly homologous with the DNA-directed RNA polymerase gene ECBD_2906 from E. coli strain <t>BL21-DE3</t>
    Bl21 T7, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore competent e coli novablue
    Examples of the differences between the original RefSeq annotation and our reannotation. a In the reannotation, one pseudogene (RS16270) was identified as two genes, ins A and ins B, which show strong homology to the insertion element protein, IS1. b In the reannotation, two pseudogenes were re-identified as two genes ( lac Z1 and lac Z2), whereas the hypothetical protein was reannotated and shown to be highly homologous with the DNA-directed RNA polymerase gene ECBD_2906 from E. coli strain <t>BL21-DE3</t>
    Competent E Coli Novablue, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Thermo Fisher mc1061 p3 competent e coli
    Examples of the differences between the original RefSeq annotation and our reannotation. a In the reannotation, one pseudogene (RS16270) was identified as two genes, ins A and ins B, which show strong homology to the insertion element protein, IS1. b In the reannotation, two pseudogenes were re-identified as two genes ( lac Z1 and lac Z2), whereas the hypothetical protein was reannotated and shown to be highly homologous with the DNA-directed RNA polymerase gene ECBD_2906 from E. coli strain <t>BL21-DE3</t>
    Mc1061 P3 Competent E Coli, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    A; Diagram of the construction of the entry and expression clones. The HepG2 cell line was used as a source of FVII cDNA. Blunt-end PCR product was TOPO-cloned into the pENTR TOPO/D vector. The construct was used to transform competent  E. coli  and positive clones were selected on LB medium containing an appropriate antibiotic. This construct is called the entry clone. Next, LR recombinant reaction was carried out between pENTR TOPO/D-FVII and pDEST26 vector to construct the expression clone. The resulting pDEST26-FVII, which is called the expression clone, was transfected into CHO cells. Stable clones expressing recombinant FVII were established in the presence of geneticin. B; pDEST26 vector. C; N-terminal sequence of the fusion protein.

    Journal: Blood Transfusion

    Article Title: Expression and purification of recombinant human coagulation factor VII fused to a histidine tag using Gateway technology

    doi: 10.2450/2009.0081-08

    Figure Lengend Snippet: A; Diagram of the construction of the entry and expression clones. The HepG2 cell line was used as a source of FVII cDNA. Blunt-end PCR product was TOPO-cloned into the pENTR TOPO/D vector. The construct was used to transform competent E. coli and positive clones were selected on LB medium containing an appropriate antibiotic. This construct is called the entry clone. Next, LR recombinant reaction was carried out between pENTR TOPO/D-FVII and pDEST26 vector to construct the expression clone. The resulting pDEST26-FVII, which is called the expression clone, was transfected into CHO cells. Stable clones expressing recombinant FVII were established in the presence of geneticin. B; pDEST26 vector. C; N-terminal sequence of the fusion protein.

    Article Snippet: The reaction was then placed on ice and the pENTR TOPO/D-FVII construct was transformed to competent E. coli according to the manufacturer’s protocol (Invitrogen, USA).

    Techniques: Expressing, Clone Assay, Polymerase Chain Reaction, Plasmid Preparation, Construct, Recombinant, Transfection, Sequencing

    Examples of the differences between the original RefSeq annotation and our reannotation. a In the reannotation, one pseudogene (RS16270) was identified as two genes, ins A and ins B, which show strong homology to the insertion element protein, IS1. b In the reannotation, two pseudogenes were re-identified as two genes ( lac Z1 and lac Z2), whereas the hypothetical protein was reannotated and shown to be highly homologous with the DNA-directed RNA polymerase gene ECBD_2906 from E. coli strain BL21-DE3

    Journal: BMC Genomics

    Article Title: Genome re-sequencing and reannotation of the Escherichia coli ER2566 strain and transcriptome sequencing under overexpression conditions

    doi: 10.1186/s12864-020-06818-1

    Figure Lengend Snippet: Examples of the differences between the original RefSeq annotation and our reannotation. a In the reannotation, one pseudogene (RS16270) was identified as two genes, ins A and ins B, which show strong homology to the insertion element protein, IS1. b In the reannotation, two pseudogenes were re-identified as two genes ( lac Z1 and lac Z2), whereas the hypothetical protein was reannotated and shown to be highly homologous with the DNA-directed RNA polymerase gene ECBD_2906 from E. coli strain BL21-DE3

    Article Snippet: Background The Escherichia coli expression system is one of the most well-characterized classical expression systems for recombinant protein expression in biological science.

    Techniques: