competent e. coli cells Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    New England Biolabs dh5α competent e coli cells
    Dh5α Competent E Coli Cells, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dh5α competent e coli cells/product/New England Biolabs
    Average 99 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    dh5α competent e coli cells - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher electro competent e coli cells
    Electro Competent E Coli Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/electro competent e coli cells/product/Thermo Fisher
    Average 99 stars, based on 42 article reviews
    Price from $9.99 to $1999.99
    electro competent e coli cells - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    92
    TaKaRa competent escherichia coli cells
    Competent Escherichia Coli Cells, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/competent escherichia coli cells/product/TaKaRa
    Average 92 stars, based on 59 article reviews
    Price from $9.99 to $1999.99
    competent escherichia coli cells - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    92
    Agilent technologies competent e coli cells
    Competent E Coli Cells, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/competent e coli cells/product/Agilent technologies
    Average 92 stars, based on 83 article reviews
    Price from $9.99 to $1999.99
    competent e coli cells - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    92
    TransGen biotech co competent e coli cells
    Competent E Coli Cells, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/competent e coli cells/product/TransGen biotech co
    Average 92 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    competent e coli cells - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    92
    Genlantis competent e coli cells
    Competent E Coli Cells, supplied by Genlantis, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/competent e coli cells/product/Genlantis
    Average 92 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    competent e coli cells - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    90
    tiangen biotech co competent e coli cells
    Competent E Coli Cells, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 90/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/competent e coli cells/product/tiangen biotech co
    Average 90 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    competent e coli cells - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    92
    Stratagene competent e coli cells
    Competent E Coli Cells, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/competent e coli cells/product/Stratagene
    Average 92 stars, based on 56 article reviews
    Price from $9.99 to $1999.99
    competent e coli cells - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    93
    Thermo Fisher competent escherichia coli cells
    Competent Escherichia Coli Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1955 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/competent escherichia coli cells/product/Thermo Fisher
    Average 93 stars, based on 1955 article reviews
    Price from $9.99 to $1999.99
    competent escherichia coli cells - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    97
    Millipore competent e coli cells
    Purification of recombinant Wo-ORF1 proteins and serotyping by indirect ELISA. Panels ( a , b ) show, respectively, the purification and characterization of Wo-ORF1-LC and of Wo-ORF1-HC, as recombinant proteins. Full-length Wo-ORF-LC or Wo-ORF1-HC (arrows) were expressed in competent  E. coli  cells. Total lysates “L” were purified with an affinity HisTrap HP column. The eluted fractions “E”, followed by progressive numbers, were pooled and dialysed overnight “D”. SDS-PAGE gel was stained with SimplyBlue™ SafeStain. These images are representative of three independent expression and purifications experiments. ( c ) The seven BoNT serotypes or Wo-ORF1-LC or Wo-ORF1-HC were incubated with BoNT serotype specific polyclonal antisera provided by the CDC, Atlanta. The ELISA test was developed using ABTS. The mean optical density at 405 nm of the average of three independent sets of experiments each one consisting of triplicates. The values obtained with the standard sera (anti serotype A, BoNT/A; anti serotype B, BoNT/B; etc.) were taken as 100% and the values obtained with the  Weissella  proteins expressed as percentages. Bars represent S.D. values.
    Competent E Coli Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 80 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/competent e coli cells/product/Millipore
    Average 97 stars, based on 80 article reviews
    Price from $9.99 to $1999.99
    competent e coli cells - by Bioz Stars, 2020-07
    97/100 stars
      Buy from Supplier

    85
    Millipore novasingles competent e coli cells
    Purification of recombinant Wo-ORF1 proteins and serotyping by indirect ELISA. Panels ( a , b ) show, respectively, the purification and characterization of Wo-ORF1-LC and of Wo-ORF1-HC, as recombinant proteins. Full-length Wo-ORF-LC or Wo-ORF1-HC (arrows) were expressed in competent  E. coli  cells. Total lysates “L” were purified with an affinity HisTrap HP column. The eluted fractions “E”, followed by progressive numbers, were pooled and dialysed overnight “D”. SDS-PAGE gel was stained with SimplyBlue™ SafeStain. These images are representative of three independent expression and purifications experiments. ( c ) The seven BoNT serotypes or Wo-ORF1-LC or Wo-ORF1-HC were incubated with BoNT serotype specific polyclonal antisera provided by the CDC, Atlanta. The ELISA test was developed using ABTS. The mean optical density at 405 nm of the average of three independent sets of experiments each one consisting of triplicates. The values obtained with the standard sera (anti serotype A, BoNT/A; anti serotype B, BoNT/B; etc.) were taken as 100% and the values obtained with the  Weissella  proteins expressed as percentages. Bars represent S.D. values.
    Novasingles Competent E Coli Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/novasingles competent e coli cells/product/Millipore
    Average 85 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    novasingles competent e coli cells - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    88
    Stratagene sure2 competent e coli cells
    Purification of recombinant Wo-ORF1 proteins and serotyping by indirect ELISA. Panels ( a , b ) show, respectively, the purification and characterization of Wo-ORF1-LC and of Wo-ORF1-HC, as recombinant proteins. Full-length Wo-ORF-LC or Wo-ORF1-HC (arrows) were expressed in competent  E. coli  cells. Total lysates “L” were purified with an affinity HisTrap HP column. The eluted fractions “E”, followed by progressive numbers, were pooled and dialysed overnight “D”. SDS-PAGE gel was stained with SimplyBlue™ SafeStain. These images are representative of three independent expression and purifications experiments. ( c ) The seven BoNT serotypes or Wo-ORF1-LC or Wo-ORF1-HC were incubated with BoNT serotype specific polyclonal antisera provided by the CDC, Atlanta. The ELISA test was developed using ABTS. The mean optical density at 405 nm of the average of three independent sets of experiments each one consisting of triplicates. The values obtained with the standard sera (anti serotype A, BoNT/A; anti serotype B, BoNT/B; etc.) were taken as 100% and the values obtained with the  Weissella  proteins expressed as percentages. Bars represent S.D. values.
    Sure2 Competent E Coli Cells, supplied by Stratagene, used in various techniques. Bioz Stars score: 88/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sure2 competent e coli cells/product/Stratagene
    Average 88 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    sure2 competent e coli cells - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    91
    Thermo Fisher pir1 competent e coli cells
    Purification of recombinant Wo-ORF1 proteins and serotyping by indirect ELISA. Panels ( a , b ) show, respectively, the purification and characterization of Wo-ORF1-LC and of Wo-ORF1-HC, as recombinant proteins. Full-length Wo-ORF-LC or Wo-ORF1-HC (arrows) were expressed in competent  E. coli  cells. Total lysates “L” were purified with an affinity HisTrap HP column. The eluted fractions “E”, followed by progressive numbers, were pooled and dialysed overnight “D”. SDS-PAGE gel was stained with SimplyBlue™ SafeStain. These images are representative of three independent expression and purifications experiments. ( c ) The seven BoNT serotypes or Wo-ORF1-LC or Wo-ORF1-HC were incubated with BoNT serotype specific polyclonal antisera provided by the CDC, Atlanta. The ELISA test was developed using ABTS. The mean optical density at 405 nm of the average of three independent sets of experiments each one consisting of triplicates. The values obtained with the standard sera (anti serotype A, BoNT/A; anti serotype B, BoNT/B; etc.) were taken as 100% and the values obtained with the  Weissella  proteins expressed as percentages. Bars represent S.D. values.
    Pir1 Competent E Coli Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pir1 competent e coli cells/product/Thermo Fisher
    Average 91 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    pir1 competent e coli cells - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    88
    Thermo Fisher dh5α competent escherichia coli cells
    Purification of recombinant Wo-ORF1 proteins and serotyping by indirect ELISA. Panels ( a , b ) show, respectively, the purification and characterization of Wo-ORF1-LC and of Wo-ORF1-HC, as recombinant proteins. Full-length Wo-ORF-LC or Wo-ORF1-HC (arrows) were expressed in competent  E. coli  cells. Total lysates “L” were purified with an affinity HisTrap HP column. The eluted fractions “E”, followed by progressive numbers, were pooled and dialysed overnight “D”. SDS-PAGE gel was stained with SimplyBlue™ SafeStain. These images are representative of three independent expression and purifications experiments. ( c ) The seven BoNT serotypes or Wo-ORF1-LC or Wo-ORF1-HC were incubated with BoNT serotype specific polyclonal antisera provided by the CDC, Atlanta. The ELISA test was developed using ABTS. The mean optical density at 405 nm of the average of three independent sets of experiments each one consisting of triplicates. The values obtained with the standard sera (anti serotype A, BoNT/A; anti serotype B, BoNT/B; etc.) were taken as 100% and the values obtained with the  Weissella  proteins expressed as percentages. Bars represent S.D. values.
    Dh5α Competent Escherichia Coli Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 97 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dh5α competent escherichia coli cells/product/Thermo Fisher
    Average 88 stars, based on 97 article reviews
    Price from $9.99 to $1999.99
    dh5α competent escherichia coli cells - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    86
    Stratagene super competent e coli cells
    Purification of recombinant Wo-ORF1 proteins and serotyping by indirect ELISA. Panels ( a , b ) show, respectively, the purification and characterization of Wo-ORF1-LC and of Wo-ORF1-HC, as recombinant proteins. Full-length Wo-ORF-LC or Wo-ORF1-HC (arrows) were expressed in competent  E. coli  cells. Total lysates “L” were purified with an affinity HisTrap HP column. The eluted fractions “E”, followed by progressive numbers, were pooled and dialysed overnight “D”. SDS-PAGE gel was stained with SimplyBlue™ SafeStain. These images are representative of three independent expression and purifications experiments. ( c ) The seven BoNT serotypes or Wo-ORF1-LC or Wo-ORF1-HC were incubated with BoNT serotype specific polyclonal antisera provided by the CDC, Atlanta. The ELISA test was developed using ABTS. The mean optical density at 405 nm of the average of three independent sets of experiments each one consisting of triplicates. The values obtained with the standard sera (anti serotype A, BoNT/A; anti serotype B, BoNT/B; etc.) were taken as 100% and the values obtained with the  Weissella  proteins expressed as percentages. Bars represent S.D. values.
    Super Competent E Coli Cells, supplied by Stratagene, used in various techniques. Bioz Stars score: 86/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/super competent e coli cells/product/Stratagene
    Average 86 stars, based on 21 article reviews
    Price from $9.99 to $1999.99
    super competent e coli cells - by Bioz Stars, 2020-07
    86/100 stars
      Buy from Supplier

    93
    Thermo Fisher dh10bac competent e coli cells
    Purification of recombinant Wo-ORF1 proteins and serotyping by indirect ELISA. Panels ( a , b ) show, respectively, the purification and characterization of Wo-ORF1-LC and of Wo-ORF1-HC, as recombinant proteins. Full-length Wo-ORF-LC or Wo-ORF1-HC (arrows) were expressed in competent  E. coli  cells. Total lysates “L” were purified with an affinity HisTrap HP column. The eluted fractions “E”, followed by progressive numbers, were pooled and dialysed overnight “D”. SDS-PAGE gel was stained with SimplyBlue™ SafeStain. These images are representative of three independent expression and purifications experiments. ( c ) The seven BoNT serotypes or Wo-ORF1-LC or Wo-ORF1-HC were incubated with BoNT serotype specific polyclonal antisera provided by the CDC, Atlanta. The ELISA test was developed using ABTS. The mean optical density at 405 nm of the average of three independent sets of experiments each one consisting of triplicates. The values obtained with the standard sera (anti serotype A, BoNT/A; anti serotype B, BoNT/B; etc.) were taken as 100% and the values obtained with the  Weissella  proteins expressed as percentages. Bars represent S.D. values.
    Dh10bac Competent E Coli Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 82 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dh10bac competent e coli cells/product/Thermo Fisher
    Average 93 stars, based on 82 article reviews
    Price from $9.99 to $1999.99
    dh10bac competent e coli cells - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    92
    Promega krx competent e coli cells
    Purification of recombinant Wo-ORF1 proteins and serotyping by indirect ELISA. Panels ( a , b ) show, respectively, the purification and characterization of Wo-ORF1-LC and of Wo-ORF1-HC, as recombinant proteins. Full-length Wo-ORF-LC or Wo-ORF1-HC (arrows) were expressed in competent  E. coli  cells. Total lysates “L” were purified with an affinity HisTrap HP column. The eluted fractions “E”, followed by progressive numbers, were pooled and dialysed overnight “D”. SDS-PAGE gel was stained with SimplyBlue™ SafeStain. These images are representative of three independent expression and purifications experiments. ( c ) The seven BoNT serotypes or Wo-ORF1-LC or Wo-ORF1-HC were incubated with BoNT serotype specific polyclonal antisera provided by the CDC, Atlanta. The ELISA test was developed using ABTS. The mean optical density at 405 nm of the average of three independent sets of experiments each one consisting of triplicates. The values obtained with the standard sera (anti serotype A, BoNT/A; anti serotype B, BoNT/B; etc.) were taken as 100% and the values obtained with the  Weissella  proteins expressed as percentages. Bars represent S.D. values.
    Krx Competent E Coli Cells, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/krx competent e coli cells/product/Promega
    Average 92 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    krx competent e coli cells - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    92
    Promega dh5α competent e coli cells
    Purification of recombinant Wo-ORF1 proteins and serotyping by indirect ELISA. Panels ( a , b ) show, respectively, the purification and characterization of Wo-ORF1-LC and of Wo-ORF1-HC, as recombinant proteins. Full-length Wo-ORF-LC or Wo-ORF1-HC (arrows) were expressed in competent  E. coli  cells. Total lysates “L” were purified with an affinity HisTrap HP column. The eluted fractions “E”, followed by progressive numbers, were pooled and dialysed overnight “D”. SDS-PAGE gel was stained with SimplyBlue™ SafeStain. These images are representative of three independent expression and purifications experiments. ( c ) The seven BoNT serotypes or Wo-ORF1-LC or Wo-ORF1-HC were incubated with BoNT serotype specific polyclonal antisera provided by the CDC, Atlanta. The ELISA test was developed using ABTS. The mean optical density at 405 nm of the average of three independent sets of experiments each one consisting of triplicates. The values obtained with the standard sera (anti serotype A, BoNT/A; anti serotype B, BoNT/B; etc.) were taken as 100% and the values obtained with the  Weissella  proteins expressed as percentages. Bars represent S.D. values.
    Dh5α Competent E Coli Cells, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dh5α competent e coli cells/product/Promega
    Average 92 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    dh5α competent e coli cells - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    92
    Stratagene de3 competent e coli cells
    Purification of recombinant Wo-ORF1 proteins and serotyping by indirect ELISA. Panels ( a , b ) show, respectively, the purification and characterization of Wo-ORF1-LC and of Wo-ORF1-HC, as recombinant proteins. Full-length Wo-ORF-LC or Wo-ORF1-HC (arrows) were expressed in competent  E. coli  cells. Total lysates “L” were purified with an affinity HisTrap HP column. The eluted fractions “E”, followed by progressive numbers, were pooled and dialysed overnight “D”. SDS-PAGE gel was stained with SimplyBlue™ SafeStain. These images are representative of three independent expression and purifications experiments. ( c ) The seven BoNT serotypes or Wo-ORF1-LC or Wo-ORF1-HC were incubated with BoNT serotype specific polyclonal antisera provided by the CDC, Atlanta. The ELISA test was developed using ABTS. The mean optical density at 405 nm of the average of three independent sets of experiments each one consisting of triplicates. The values obtained with the standard sera (anti serotype A, BoNT/A; anti serotype B, BoNT/B; etc.) were taken as 100% and the values obtained with the  Weissella  proteins expressed as percentages. Bars represent S.D. values.
    De3 Competent E Coli Cells, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/de3 competent e coli cells/product/Stratagene
    Average 92 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    de3 competent e coli cells - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    90
    Lucigen Corp 10g competent e coli cells
    Purification of recombinant Wo-ORF1 proteins and serotyping by indirect ELISA. Panels ( a , b ) show, respectively, the purification and characterization of Wo-ORF1-LC and of Wo-ORF1-HC, as recombinant proteins. Full-length Wo-ORF-LC or Wo-ORF1-HC (arrows) were expressed in competent  E. coli  cells. Total lysates “L” were purified with an affinity HisTrap HP column. The eluted fractions “E”, followed by progressive numbers, were pooled and dialysed overnight “D”. SDS-PAGE gel was stained with SimplyBlue™ SafeStain. These images are representative of three independent expression and purifications experiments. ( c ) The seven BoNT serotypes or Wo-ORF1-LC or Wo-ORF1-HC were incubated with BoNT serotype specific polyclonal antisera provided by the CDC, Atlanta. The ELISA test was developed using ABTS. The mean optical density at 405 nm of the average of three independent sets of experiments each one consisting of triplicates. The values obtained with the standard sera (anti serotype A, BoNT/A; anti serotype B, BoNT/B; etc.) were taken as 100% and the values obtained with the  Weissella  proteins expressed as percentages. Bars represent S.D. values.
    10g Competent E Coli Cells, supplied by Lucigen Corp, used in various techniques. Bioz Stars score: 90/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/10g competent e coli cells/product/Lucigen Corp
    Average 90 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    10g competent e coli cells - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    85
    Thermo Fisher invαf competent e coli cells
    Purification of recombinant Wo-ORF1 proteins and serotyping by indirect ELISA. Panels ( a , b ) show, respectively, the purification and characterization of Wo-ORF1-LC and of Wo-ORF1-HC, as recombinant proteins. Full-length Wo-ORF-LC or Wo-ORF1-HC (arrows) were expressed in competent  E. coli  cells. Total lysates “L” were purified with an affinity HisTrap HP column. The eluted fractions “E”, followed by progressive numbers, were pooled and dialysed overnight “D”. SDS-PAGE gel was stained with SimplyBlue™ SafeStain. These images are representative of three independent expression and purifications experiments. ( c ) The seven BoNT serotypes or Wo-ORF1-LC or Wo-ORF1-HC were incubated with BoNT serotype specific polyclonal antisera provided by the CDC, Atlanta. The ELISA test was developed using ABTS. The mean optical density at 405 nm of the average of three independent sets of experiments each one consisting of triplicates. The values obtained with the standard sera (anti serotype A, BoNT/A; anti serotype B, BoNT/B; etc.) were taken as 100% and the values obtained with the  Weissella  proteins expressed as percentages. Bars represent S.D. values.
    Invαf Competent E Coli Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/invαf competent e coli cells/product/Thermo Fisher
    Average 85 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    invαf competent e coli cells - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    93
    tiangen biotech co top10 competent e coli cells
    Purification of recombinant Wo-ORF1 proteins and serotyping by indirect ELISA. Panels ( a , b ) show, respectively, the purification and characterization of Wo-ORF1-LC and of Wo-ORF1-HC, as recombinant proteins. Full-length Wo-ORF-LC or Wo-ORF1-HC (arrows) were expressed in competent  E. coli  cells. Total lysates “L” were purified with an affinity HisTrap HP column. The eluted fractions “E”, followed by progressive numbers, were pooled and dialysed overnight “D”. SDS-PAGE gel was stained with SimplyBlue™ SafeStain. These images are representative of three independent expression and purifications experiments. ( c ) The seven BoNT serotypes or Wo-ORF1-LC or Wo-ORF1-HC were incubated with BoNT serotype specific polyclonal antisera provided by the CDC, Atlanta. The ELISA test was developed using ABTS. The mean optical density at 405 nm of the average of three independent sets of experiments each one consisting of triplicates. The values obtained with the standard sera (anti serotype A, BoNT/A; anti serotype B, BoNT/B; etc.) were taken as 100% and the values obtained with the  Weissella  proteins expressed as percentages. Bars represent S.D. values.
    Top10 Competent E Coli Cells, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 93/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/top10 competent e coli cells/product/tiangen biotech co
    Average 93 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    top10 competent e coli cells - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    88
    Thermo Fisher bl 21 competent e coli cells
    Purification of recombinant Wo-ORF1 proteins and serotyping by indirect ELISA. Panels ( a , b ) show, respectively, the purification and characterization of Wo-ORF1-LC and of Wo-ORF1-HC, as recombinant proteins. Full-length Wo-ORF-LC or Wo-ORF1-HC (arrows) were expressed in competent  E. coli  cells. Total lysates “L” were purified with an affinity HisTrap HP column. The eluted fractions “E”, followed by progressive numbers, were pooled and dialysed overnight “D”. SDS-PAGE gel was stained with SimplyBlue™ SafeStain. These images are representative of three independent expression and purifications experiments. ( c ) The seven BoNT serotypes or Wo-ORF1-LC or Wo-ORF1-HC were incubated with BoNT serotype specific polyclonal antisera provided by the CDC, Atlanta. The ELISA test was developed using ABTS. The mean optical density at 405 nm of the average of three independent sets of experiments each one consisting of triplicates. The values obtained with the standard sera (anti serotype A, BoNT/A; anti serotype B, BoNT/B; etc.) were taken as 100% and the values obtained with the  Weissella  proteins expressed as percentages. Bars represent S.D. values.
    Bl 21 Competent E Coli Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bl 21 competent e coli cells/product/Thermo Fisher
    Average 88 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    bl 21 competent e coli cells - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    88
    Agilent technologies xl1 red competent e coli cells
    Purification of recombinant Wo-ORF1 proteins and serotyping by indirect ELISA. Panels ( a , b ) show, respectively, the purification and characterization of Wo-ORF1-LC and of Wo-ORF1-HC, as recombinant proteins. Full-length Wo-ORF-LC or Wo-ORF1-HC (arrows) were expressed in competent  E. coli  cells. Total lysates “L” were purified with an affinity HisTrap HP column. The eluted fractions “E”, followed by progressive numbers, were pooled and dialysed overnight “D”. SDS-PAGE gel was stained with SimplyBlue™ SafeStain. These images are representative of three independent expression and purifications experiments. ( c ) The seven BoNT serotypes or Wo-ORF1-LC or Wo-ORF1-HC were incubated with BoNT serotype specific polyclonal antisera provided by the CDC, Atlanta. The ELISA test was developed using ABTS. The mean optical density at 405 nm of the average of three independent sets of experiments each one consisting of triplicates. The values obtained with the standard sera (anti serotype A, BoNT/A; anti serotype B, BoNT/B; etc.) were taken as 100% and the values obtained with the  Weissella  proteins expressed as percentages. Bars represent S.D. values.
    Xl1 Red Competent E Coli Cells, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xl1 red competent e coli cells/product/Agilent technologies
    Average 88 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    xl1 red competent e coli cells - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    88
    Agilent technologies bl21 de3 competent e coli cells
    Purification of recombinant Wo-ORF1 proteins and serotyping by indirect ELISA. Panels ( a , b ) show, respectively, the purification and characterization of Wo-ORF1-LC and of Wo-ORF1-HC, as recombinant proteins. Full-length Wo-ORF-LC or Wo-ORF1-HC (arrows) were expressed in competent  E. coli  cells. Total lysates “L” were purified with an affinity HisTrap HP column. The eluted fractions “E”, followed by progressive numbers, were pooled and dialysed overnight “D”. SDS-PAGE gel was stained with SimplyBlue™ SafeStain. These images are representative of three independent expression and purifications experiments. ( c ) The seven BoNT serotypes or Wo-ORF1-LC or Wo-ORF1-HC were incubated with BoNT serotype specific polyclonal antisera provided by the CDC, Atlanta. The ELISA test was developed using ABTS. The mean optical density at 405 nm of the average of three independent sets of experiments each one consisting of triplicates. The values obtained with the standard sera (anti serotype A, BoNT/A; anti serotype B, BoNT/B; etc.) were taken as 100% and the values obtained with the  Weissella  proteins expressed as percentages. Bars represent S.D. values.
    Bl21 De3 Competent E Coli Cells, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 88/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bl21 de3 competent e coli cells/product/Agilent technologies
    Average 88 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    bl21 de3 competent e coli cells - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    Image Search Results


    Purification of recombinant Wo-ORF1 proteins and serotyping by indirect ELISA. Panels ( a , b ) show, respectively, the purification and characterization of Wo-ORF1-LC and of Wo-ORF1-HC, as recombinant proteins. Full-length Wo-ORF-LC or Wo-ORF1-HC (arrows) were expressed in competent  E. coli  cells. Total lysates “L” were purified with an affinity HisTrap HP column. The eluted fractions “E”, followed by progressive numbers, were pooled and dialysed overnight “D”. SDS-PAGE gel was stained with SimplyBlue™ SafeStain. These images are representative of three independent expression and purifications experiments. ( c ) The seven BoNT serotypes or Wo-ORF1-LC or Wo-ORF1-HC were incubated with BoNT serotype specific polyclonal antisera provided by the CDC, Atlanta. The ELISA test was developed using ABTS. The mean optical density at 405 nm of the average of three independent sets of experiments each one consisting of triplicates. The values obtained with the standard sera (anti serotype A, BoNT/A; anti serotype B, BoNT/B; etc.) were taken as 100% and the values obtained with the  Weissella  proteins expressed as percentages. Bars represent S.D. values.

    Journal: Scientific Reports

    Article Title: The first non Clostridial botulinum-like toxin cleaves VAMP within the juxtamembrane domain

    doi: 10.1038/srep30257

    Figure Lengend Snippet: Purification of recombinant Wo-ORF1 proteins and serotyping by indirect ELISA. Panels ( a , b ) show, respectively, the purification and characterization of Wo-ORF1-LC and of Wo-ORF1-HC, as recombinant proteins. Full-length Wo-ORF-LC or Wo-ORF1-HC (arrows) were expressed in competent E. coli cells. Total lysates “L” were purified with an affinity HisTrap HP column. The eluted fractions “E”, followed by progressive numbers, were pooled and dialysed overnight “D”. SDS-PAGE gel was stained with SimplyBlue™ SafeStain. These images are representative of three independent expression and purifications experiments. ( c ) The seven BoNT serotypes or Wo-ORF1-LC or Wo-ORF1-HC were incubated with BoNT serotype specific polyclonal antisera provided by the CDC, Atlanta. The ELISA test was developed using ABTS. The mean optical density at 405 nm of the average of three independent sets of experiments each one consisting of triplicates. The values obtained with the standard sera (anti serotype A, BoNT/A; anti serotype B, BoNT/B; etc.) were taken as 100% and the values obtained with the Weissella proteins expressed as percentages. Bars represent S.D. values.

    Article Snippet: They were transformed in competent E. coli cells (EMD Millipore), and bacteria were grown for 4 hours in the presence of 1 mM IPTG.

    Techniques: Purification, Recombinant, Indirect ELISA, SDS Page, Staining, Expressing, Incubation, Enzyme-linked Immunosorbent Assay