competent e. coli Search Results


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  • 99
    New England Biolabs competent e coli
    Competent E Coli, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 399 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher competent e coli
    A; Diagram of the construction of the entry and expression clones. The HepG2 cell line was used as a source of FVII cDNA. Blunt-end PCR product was TOPO-cloned into the pENTR TOPO/D vector. The construct was used to transform competent  E. coli  and positive clones were selected on LB medium containing an appropriate antibiotic. This construct is called the entry clone. Next, LR recombinant reaction was carried out between pENTR TOPO/D-FVII and pDEST26 vector to construct the expression clone. The resulting pDEST26-FVII, which is called the expression clone, was transfected into CHO cells. Stable clones expressing recombinant FVII were established in the presence of geneticin. B; pDEST26 vector. C; N-terminal sequence of the fusion protein.
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    New England Biolabs competent e coli bl21
    Recombinant c3B protein expression, purification, and detection. (A) Coomassie blue-stained polyacrylamide gel of recombinant MBP-c3B protein expressed in BL21  E. coli  and affinity purified using amylose resin. Lane M, molecular mass markers (Mark 12;
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    New England Biolabs competent e coli cells
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    New England Biolabs dcm competent e coli
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    New England Biolabs bl21 competent escherichia coli cells
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    Stratagene competent e coli
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    Avantor competent e coli
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    Millipore competent e coli
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    Meridian Life Science competent e coli
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    Bio-Rad competent e coli
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    TransGen biotech co competent e coli
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    New England Biolabs shuffle t7 competent e coli shk
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    New England Biolabs lemo21 de3 competent e coli cells
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    Lemo21 De3 Competent E Coli Cells, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs shuffle express competent e coli cells
    Recombinant c3B protein expression, purification, and detection. (A) Coomassie blue-stained polyacrylamide gel of recombinant MBP-c3B protein expressed in BL21  E. coli  and affinity purified using amylose resin. Lane M, molecular mass markers (Mark 12;
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    Thermo Fisher competent e coli dh5α
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    Millipore competent e coli b834
    Recombinant c3B protein expression, purification, and detection. (A) Coomassie blue-stained polyacrylamide gel of recombinant MBP-c3B protein expressed in BL21  E. coli  and affinity purified using amylose resin. Lane M, molecular mass markers (Mark 12;
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    Euromedex competent e coli bth101
    Recombinant c3B protein expression, purification, and detection. (A) Coomassie blue-stained polyacrylamide gel of recombinant MBP-c3B protein expressed in BL21  E. coli  and affinity purified using amylose resin. Lane M, molecular mass markers (Mark 12;
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    Thermo Fisher pir1 competent e coli
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    Pir1 Competent E Coli, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher competent e coli dh10b
    Recombinant c3B protein expression, purification, and detection. (A) Coomassie blue-stained polyacrylamide gel of recombinant MBP-c3B protein expressed in BL21  E. coli  and affinity purified using amylose resin. Lane M, molecular mass markers (Mark 12;
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    RBC Bioscience competent e coli dh5α
    Recombinant c3B protein expression, purification, and detection. (A) Coomassie blue-stained polyacrylamide gel of recombinant MBP-c3B protein expressed in BL21  E. coli  and affinity purified using amylose resin. Lane M, molecular mass markers (Mark 12;
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    Thermo Fisher competent e coli topo10f
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    Thermo Fisher mc1061 p3 competent e coli
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    Millipore competent e coli rosetta
    Recombinant c3B protein expression, purification, and detection. (A) Coomassie blue-stained polyacrylamide gel of recombinant MBP-c3B protein expressed in BL21  E. coli  and affinity purified using amylose resin. Lane M, molecular mass markers (Mark 12;
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    Promega dh5α competent e coli
    Recombinant c3B protein expression, purification, and detection. (A) Coomassie blue-stained polyacrylamide gel of recombinant MBP-c3B protein expressed in BL21  E. coli  and affinity purified using amylose resin. Lane M, molecular mass markers (Mark 12;
    Dh5α Competent E Coli, supplied by Promega, used in various techniques. Bioz Stars score: 95/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa competent e coli dh5α
    Recombinant c3B protein expression, purification, and detection. (A) Coomassie blue-stained polyacrylamide gel of recombinant MBP-c3B protein expressed in BL21  E. coli  and affinity purified using amylose resin. Lane M, molecular mass markers (Mark 12;
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    Thermo Fisher dh10bac competent e coli
    Recombinant c3B protein expression, purification, and detection. (A) Coomassie blue-stained polyacrylamide gel of recombinant MBP-c3B protein expressed in BL21  E. coli  and affinity purified using amylose resin. Lane M, molecular mass markers (Mark 12;
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    Genlantis solubl21 competent e coli
    Recombinant c3B protein expression, purification, and detection. (A) Coomassie blue-stained polyacrylamide gel of recombinant MBP-c3B protein expressed in BL21  E. coli  and affinity purified using amylose resin. Lane M, molecular mass markers (Mark 12;
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    Millipore competent e coli bl21
    Recombinant c3B protein expression, purification, and detection. (A) Coomassie blue-stained polyacrylamide gel of recombinant MBP-c3B protein expressed in BL21  E. coli  and affinity purified using amylose resin. Lane M, molecular mass markers (Mark 12;
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    ATCC competent e coli mg1655
    Recombinant c3B protein expression, purification, and detection. (A) Coomassie blue-stained polyacrylamide gel of recombinant MBP-c3B protein expressed in BL21  E. coli  and affinity purified using amylose resin. Lane M, molecular mass markers (Mark 12;
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    Image Search Results


    A; Diagram of the construction of the entry and expression clones. The HepG2 cell line was used as a source of FVII cDNA. Blunt-end PCR product was TOPO-cloned into the pENTR TOPO/D vector. The construct was used to transform competent  E. coli  and positive clones were selected on LB medium containing an appropriate antibiotic. This construct is called the entry clone. Next, LR recombinant reaction was carried out between pENTR TOPO/D-FVII and pDEST26 vector to construct the expression clone. The resulting pDEST26-FVII, which is called the expression clone, was transfected into CHO cells. Stable clones expressing recombinant FVII were established in the presence of geneticin. B; pDEST26 vector. C; N-terminal sequence of the fusion protein.

    Journal: Blood Transfusion

    Article Title: Expression and purification of recombinant human coagulation factor VII fused to a histidine tag using Gateway technology

    doi: 10.2450/2009.0081-08

    Figure Lengend Snippet: A; Diagram of the construction of the entry and expression clones. The HepG2 cell line was used as a source of FVII cDNA. Blunt-end PCR product was TOPO-cloned into the pENTR TOPO/D vector. The construct was used to transform competent E. coli and positive clones were selected on LB medium containing an appropriate antibiotic. This construct is called the entry clone. Next, LR recombinant reaction was carried out between pENTR TOPO/D-FVII and pDEST26 vector to construct the expression clone. The resulting pDEST26-FVII, which is called the expression clone, was transfected into CHO cells. Stable clones expressing recombinant FVII were established in the presence of geneticin. B; pDEST26 vector. C; N-terminal sequence of the fusion protein.

    Article Snippet: The reaction was then placed on ice and the pENTR TOPO/D-FVII construct was transformed to competent E. coli according to the manufacturer’s protocol (Invitrogen, USA).

    Techniques: Expressing, Clone Assay, Polymerase Chain Reaction, Plasmid Preparation, Construct, Recombinant, Transfection, Sequencing

    Recombinant c3B protein expression, purification, and detection. (A) Coomassie blue-stained polyacrylamide gel of recombinant MBP-c3B protein expressed in BL21  E. coli  and affinity purified using amylose resin. Lane M, molecular mass markers (Mark 12;

    Journal: Journal of Clinical Microbiology

    Article Title: Pan-Serotype Diagnostic for Foot-and-Mouth Disease Using the Consensus Antigen of Nonstructural Protein 3B

    doi: 10.1128/JCM.03491-14

    Figure Lengend Snippet: Recombinant c3B protein expression, purification, and detection. (A) Coomassie blue-stained polyacrylamide gel of recombinant MBP-c3B protein expressed in BL21 E. coli and affinity purified using amylose resin. Lane M, molecular mass markers (Mark 12;

    Article Snippet: Chemically competent BL21 E. coli (NEB) was transformed with 10 ng pMAL-c5e or pMAL-c3B for overexpression of recombinant MBP or MBP-c3B fusion protein, according to the manufacturer's instructions.

    Techniques: Recombinant, Expressing, Purification, Staining, Affinity Purification