collagenase d Search Results


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  • 99
    Millipore type iv collangenase
    Cetuximab modulates macrophage polarization in an AOM/DSS mouse model A. Establishment of the AOM/DSS mouse model. AOM was injected intraperitoneally at 12.5 mg/kg body weight. After one week, mice were given drinking water containing 2.5% DSS for 5 days followed by 16 days of regular drinking water. After two cycles of DSS treatment, cetuximab (1 mg/mouse, twice a week) was injected intraperitoneally for a month, and the mice were then sacrificed. B. Representative images of colon tumors in normal (right), AOM/DSS (2AD) (middle), and cetuximab-treated AOM/DSS mice (2AD+cetu) (left). C. Tumor quantification. Cetuximab treatment (2AD + cetu) reduced tumor numbers compared to 2AD mice. D. p-EGFR (Y1068), EGFR, Arg1, and iNOS protein levels in normal mice, 2AD and 2AD+cetu mouse tumors were detected by Western blot. All experiments were repeated three times. E. Representative photomicrographs of immunostaining for p-EGFR (Y1068), PCNA, F4/80, Arg1, and iNOS. Scale bars: 25 μm. F. M1 marker (IL-12, iNOS) and M2 marker (IL-4, IL-10, Arg1) mRNA levels in normal mouse colon tissues, 2AD and 2AD+cetu mouse tumor tissues were evaluated by q-PCR. G. Percentages of CD11b + /F4/80 + and F4/80 + /CD206 + cells in normal, 2AD, and 2AD+cetu mice colon tissues were detected by flow cytometry. Colon tissues were cut into small pieces (1-2 mm) and incubated with <t>collagenase</t> D (1- mg/mL), dispase II (1 mg/mL), and DNase I (100 μg/mL) for 30-45 min in a shaking incubator at 37°C, and single-cell suspensions were then incubated with antibodies. Bars represent means ± SD (n = 3) for each treatment. * p
    Type Iv Collangenase, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 3319 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Worthington Biochemical collagenase d
    Cetuximab modulates macrophage polarization in an AOM/DSS mouse model A. Establishment of the AOM/DSS mouse model. AOM was injected intraperitoneally at 12.5 mg/kg body weight. After one week, mice were given drinking water containing 2.5% DSS for 5 days followed by 16 days of regular drinking water. After two cycles of DSS treatment, cetuximab (1 mg/mouse, twice a week) was injected intraperitoneally for a month, and the mice were then sacrificed. B. Representative images of colon tumors in normal (right), AOM/DSS (2AD) (middle), and cetuximab-treated AOM/DSS mice (2AD+cetu) (left). C. Tumor quantification. Cetuximab treatment (2AD + cetu) reduced tumor numbers compared to 2AD mice. D. p-EGFR (Y1068), EGFR, Arg1, and iNOS protein levels in normal mice, 2AD and 2AD+cetu mouse tumors were detected by Western blot. All experiments were repeated three times. E. Representative photomicrographs of immunostaining for p-EGFR (Y1068), PCNA, F4/80, Arg1, and iNOS. Scale bars: 25 μm. F. M1 marker (IL-12, iNOS) and M2 marker (IL-4, IL-10, Arg1) mRNA levels in normal mouse colon tissues, 2AD and 2AD+cetu mouse tumor tissues were evaluated by q-PCR. G. Percentages of CD11b + /F4/80 + and F4/80 + /CD206 + cells in normal, 2AD, and 2AD+cetu mice colon tissues were detected by flow cytometry. Colon tissues were cut into small pieces (1-2 mm) and incubated with <t>collagenase</t> D (1- mg/mL), dispase II (1 mg/mL), and DNase I (100 μg/mL) for 30-45 min in a shaking incubator at 37°C, and single-cell suspensions were then incubated with antibodies. Bars represent means ± SD (n = 3) for each treatment. * p
    Collagenase D, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 99/100, based on 263 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore collagenase a d
    Cetuximab modulates macrophage polarization in an AOM/DSS mouse model A. Establishment of the AOM/DSS mouse model. AOM was injected intraperitoneally at 12.5 mg/kg body weight. After one week, mice were given drinking water containing 2.5% DSS for 5 days followed by 16 days of regular drinking water. After two cycles of DSS treatment, cetuximab (1 mg/mouse, twice a week) was injected intraperitoneally for a month, and the mice were then sacrificed. B. Representative images of colon tumors in normal (right), AOM/DSS (2AD) (middle), and cetuximab-treated AOM/DSS mice (2AD+cetu) (left). C. Tumor quantification. Cetuximab treatment (2AD + cetu) reduced tumor numbers compared to 2AD mice. D. p-EGFR (Y1068), EGFR, Arg1, and iNOS protein levels in normal mice, 2AD and 2AD+cetu mouse tumors were detected by Western blot. All experiments were repeated three times. E. Representative photomicrographs of immunostaining for p-EGFR (Y1068), PCNA, F4/80, Arg1, and iNOS. Scale bars: 25 μm. F. M1 marker (IL-12, iNOS) and M2 marker (IL-4, IL-10, Arg1) mRNA levels in normal mouse colon tissues, 2AD and 2AD+cetu mouse tumor tissues were evaluated by q-PCR. G. Percentages of CD11b + /F4/80 + and F4/80 + /CD206 + cells in normal, 2AD, and 2AD+cetu mice colon tissues were detected by flow cytometry. Colon tissues were cut into small pieces (1-2 mm) and incubated with <t>collagenase</t> D (1- mg/mL), dispase II (1 mg/mL), and DNase I (100 μg/mL) for 30-45 min in a shaking incubator at 37°C, and single-cell suspensions were then incubated with antibodies. Bars represent means ± SD (n = 3) for each treatment. * p
    Collagenase A D, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Boehringer Mannheim collagenase d
    Cetuximab modulates macrophage polarization in an AOM/DSS mouse model A. Establishment of the AOM/DSS mouse model. AOM was injected intraperitoneally at 12.5 mg/kg body weight. After one week, mice were given drinking water containing 2.5% DSS for 5 days followed by 16 days of regular drinking water. After two cycles of DSS treatment, cetuximab (1 mg/mouse, twice a week) was injected intraperitoneally for a month, and the mice were then sacrificed. B. Representative images of colon tumors in normal (right), AOM/DSS (2AD) (middle), and cetuximab-treated AOM/DSS mice (2AD+cetu) (left). C. Tumor quantification. Cetuximab treatment (2AD + cetu) reduced tumor numbers compared to 2AD mice. D. p-EGFR (Y1068), EGFR, Arg1, and iNOS protein levels in normal mice, 2AD and 2AD+cetu mouse tumors were detected by Western blot. All experiments were repeated three times. E. Representative photomicrographs of immunostaining for p-EGFR (Y1068), PCNA, F4/80, Arg1, and iNOS. Scale bars: 25 μm. F. M1 marker (IL-12, iNOS) and M2 marker (IL-4, IL-10, Arg1) mRNA levels in normal mouse colon tissues, 2AD and 2AD+cetu mouse tumor tissues were evaluated by q-PCR. G. Percentages of CD11b + /F4/80 + and F4/80 + /CD206 + cells in normal, 2AD, and 2AD+cetu mice colon tissues were detected by flow cytometry. Colon tissues were cut into small pieces (1-2 mm) and incubated with <t>collagenase</t> D (1- mg/mL), dispase II (1 mg/mL), and DNase I (100 μg/mL) for 30-45 min in a shaking incubator at 37°C, and single-cell suspensions were then incubated with antibodies. Bars represent means ± SD (n = 3) for each treatment. * p
    Collagenase D, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 427 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Pfizer Inc collagenase d
    Cetuximab modulates macrophage polarization in an AOM/DSS mouse model A. Establishment of the AOM/DSS mouse model. AOM was injected intraperitoneally at 12.5 mg/kg body weight. After one week, mice were given drinking water containing 2.5% DSS for 5 days followed by 16 days of regular drinking water. After two cycles of DSS treatment, cetuximab (1 mg/mouse, twice a week) was injected intraperitoneally for a month, and the mice were then sacrificed. B. Representative images of colon tumors in normal (right), AOM/DSS (2AD) (middle), and cetuximab-treated AOM/DSS mice (2AD+cetu) (left). C. Tumor quantification. Cetuximab treatment (2AD + cetu) reduced tumor numbers compared to 2AD mice. D. p-EGFR (Y1068), EGFR, Arg1, and iNOS protein levels in normal mice, 2AD and 2AD+cetu mouse tumors were detected by Western blot. All experiments were repeated three times. E. Representative photomicrographs of immunostaining for p-EGFR (Y1068), PCNA, F4/80, Arg1, and iNOS. Scale bars: 25 μm. F. M1 marker (IL-12, iNOS) and M2 marker (IL-4, IL-10, Arg1) mRNA levels in normal mouse colon tissues, 2AD and 2AD+cetu mouse tumor tissues were evaluated by q-PCR. G. Percentages of CD11b + /F4/80 + and F4/80 + /CD206 + cells in normal, 2AD, and 2AD+cetu mice colon tissues were detected by flow cytometry. Colon tissues were cut into small pieces (1-2 mm) and incubated with <t>collagenase</t> D (1- mg/mL), dispase II (1 mg/mL), and DNase I (100 μg/mL) for 30-45 min in a shaking incubator at 37°C, and single-cell suspensions were then incubated with antibodies. Bars represent means ± SD (n = 3) for each treatment. * p
    Collagenase D, supplied by Pfizer Inc, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Roche collagenase d
    Enriched pancreatic ducts and pancreatic duct epithelium in culture. A: Enriched pancreatic ducts after partial digestion with <t>collagenase</t> D and filtration through 85-μm nylon mesh. Phase contrast, original magnification, ×100. B: Primary culture of duct cells embedded in rat-tail collagen gel with a nylon mesh. Expanding duct epithelium can be seen in the lattice. Original magnification, ×100. C: Electron microscopy of the cultured pancreatic duct cells. Their epithelial nature was apparent by the prominent microvillus processes along portions of the plasma membrane ( thick white arrows ) and formed tight junctions ( solid black arrows ) between the cohered cells. No secretary granules are seen to suggest acinar cell derivation.
    Collagenase D, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 11504 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson collagenase d
    Enriched pancreatic ducts and pancreatic duct epithelium in culture. A: Enriched pancreatic ducts after partial digestion with <t>collagenase</t> D and filtration through 85-μm nylon mesh. Phase contrast, original magnification, ×100. B: Primary culture of duct cells embedded in rat-tail collagen gel with a nylon mesh. Expanding duct epithelium can be seen in the lattice. Original magnification, ×100. C: Electron microscopy of the cultured pancreatic duct cells. Their epithelial nature was apparent by the prominent microvillus processes along portions of the plasma membrane ( thick white arrows ) and formed tight junctions ( solid black arrows ) between the cohered cells. No secretary granules are seen to suggest acinar cell derivation.
    Collagenase D, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 96/100, based on 114 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Thermo Fisher collagenase d
    Enriched pancreatic ducts and pancreatic duct epithelium in culture. A: Enriched pancreatic ducts after partial digestion with <t>collagenase</t> D and filtration through 85-μm nylon mesh. Phase contrast, original magnification, ×100. B: Primary culture of duct cells embedded in rat-tail collagen gel with a nylon mesh. Expanding duct epithelium can be seen in the lattice. Original magnification, ×100. C: Electron microscopy of the cultured pancreatic duct cells. Their epithelial nature was apparent by the prominent microvillus processes along portions of the plasma membrane ( thick white arrows ) and formed tight junctions ( solid black arrows ) between the cohered cells. No secretary granules are seen to suggest acinar cell derivation.
    Collagenase D, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 239 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    FUJIFILM collagenase d
    Enriched pancreatic ducts and pancreatic duct epithelium in culture. A: Enriched pancreatic ducts after partial digestion with <t>collagenase</t> D and filtration through 85-μm nylon mesh. Phase contrast, original magnification, ×100. B: Primary culture of duct cells embedded in rat-tail collagen gel with a nylon mesh. Expanding duct epithelium can be seen in the lattice. Original magnification, ×100. C: Electron microscopy of the cultured pancreatic duct cells. Their epithelial nature was apparent by the prominent microvillus processes along portions of the plasma membrane ( thick white arrows ) and formed tight junctions ( solid black arrows ) between the cohered cells. No secretary granules are seen to suggest acinar cell derivation.
    Collagenase D, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 94/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare collagenase d
    Enriched pancreatic ducts and pancreatic duct epithelium in culture. A: Enriched pancreatic ducts after partial digestion with <t>collagenase</t> D and filtration through 85-μm nylon mesh. Phase contrast, original magnification, ×100. B: Primary culture of duct cells embedded in rat-tail collagen gel with a nylon mesh. Expanding duct epithelium can be seen in the lattice. Original magnification, ×100. C: Electron microscopy of the cultured pancreatic duct cells. Their epithelial nature was apparent by the prominent microvillus processes along portions of the plasma membrane ( thick white arrows ) and formed tight junctions ( solid black arrows ) between the cohered cells. No secretary granules are seen to suggest acinar cell derivation.
    Collagenase D, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 95/100, based on 82 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Sangon Biotech collagenase d
    Enriched pancreatic ducts and pancreatic duct epithelium in culture. A: Enriched pancreatic ducts after partial digestion with <t>collagenase</t> D and filtration through 85-μm nylon mesh. Phase contrast, original magnification, ×100. B: Primary culture of duct cells embedded in rat-tail collagen gel with a nylon mesh. Expanding duct epithelium can be seen in the lattice. Original magnification, ×100. C: Electron microscopy of the cultured pancreatic duct cells. Their epithelial nature was apparent by the prominent microvillus processes along portions of the plasma membrane ( thick white arrows ) and formed tight junctions ( solid black arrows ) between the cohered cells. No secretary granules are seen to suggest acinar cell derivation.
    Collagenase D, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche collagenase b collagenase d
    Enriched pancreatic ducts and pancreatic duct epithelium in culture. A: Enriched pancreatic ducts after partial digestion with <t>collagenase</t> D and filtration through 85-μm nylon mesh. Phase contrast, original magnification, ×100. B: Primary culture of duct cells embedded in rat-tail collagen gel with a nylon mesh. Expanding duct epithelium can be seen in the lattice. Original magnification, ×100. C: Electron microscopy of the cultured pancreatic duct cells. Their epithelial nature was apparent by the prominent microvillus processes along portions of the plasma membrane ( thick white arrows ) and formed tight junctions ( solid black arrows ) between the cohered cells. No secretary granules are seen to suggest acinar cell derivation.
    Collagenase B Collagenase D, supplied by Roche, used in various techniques. Bioz Stars score: 79/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Fisher Scientific collagenase d
    Enriched pancreatic ducts and pancreatic duct epithelium in culture. A: Enriched pancreatic ducts after partial digestion with <t>collagenase</t> D and filtration through 85-μm nylon mesh. Phase contrast, original magnification, ×100. B: Primary culture of duct cells embedded in rat-tail collagen gel with a nylon mesh. Expanding duct epithelium can be seen in the lattice. Original magnification, ×100. C: Electron microscopy of the cultured pancreatic duct cells. Their epithelial nature was apparent by the prominent microvillus processes along portions of the plasma membrane ( thick white arrows ) and formed tight junctions ( solid black arrows ) between the cohered cells. No secretary granules are seen to suggest acinar cell derivation.
    Collagenase D, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Biochrom collagenase d
    Enriched pancreatic ducts and pancreatic duct epithelium in culture. A: Enriched pancreatic ducts after partial digestion with <t>collagenase</t> D and filtration through 85-μm nylon mesh. Phase contrast, original magnification, ×100. B: Primary culture of duct cells embedded in rat-tail collagen gel with a nylon mesh. Expanding duct epithelium can be seen in the lattice. Original magnification, ×100. C: Electron microscopy of the cultured pancreatic duct cells. Their epithelial nature was apparent by the prominent microvillus processes along portions of the plasma membrane ( thick white arrows ) and formed tight junctions ( solid black arrows ) between the cohered cells. No secretary granules are seen to suggest acinar cell derivation.
    Collagenase D, supplied by Biochrom, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck KGaA collagenase d
    Enriched pancreatic ducts and pancreatic duct epithelium in culture. A: Enriched pancreatic ducts after partial digestion with <t>collagenase</t> D and filtration through 85-μm nylon mesh. Phase contrast, original magnification, ×100. B: Primary culture of duct cells embedded in rat-tail collagen gel with a nylon mesh. Expanding duct epithelium can be seen in the lattice. Original magnification, ×100. C: Electron microscopy of the cultured pancreatic duct cells. Their epithelial nature was apparent by the prominent microvillus processes along portions of the plasma membrane ( thick white arrows ) and formed tight junctions ( solid black arrows ) between the cohered cells. No secretary granules are seen to suggest acinar cell derivation.
    Collagenase D, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Boehringer Mannheim collagenase type d
    Enriched pancreatic ducts and pancreatic duct epithelium in culture. A: Enriched pancreatic ducts after partial digestion with <t>collagenase</t> D and filtration through 85-μm nylon mesh. Phase contrast, original magnification, ×100. B: Primary culture of duct cells embedded in rat-tail collagen gel with a nylon mesh. Expanding duct epithelium can be seen in the lattice. Original magnification, ×100. C: Electron microscopy of the cultured pancreatic duct cells. Their epithelial nature was apparent by the prominent microvillus processes along portions of the plasma membrane ( thick white arrows ) and formed tight junctions ( solid black arrows ) between the cohered cells. No secretary granules are seen to suggest acinar cell derivation.
    Collagenase Type D, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche collagenase type d
    Enriched pancreatic ducts and pancreatic duct epithelium in culture. A: Enriched pancreatic ducts after partial digestion with <t>collagenase</t> D and filtration through 85-μm nylon mesh. Phase contrast, original magnification, ×100. B: Primary culture of duct cells embedded in rat-tail collagen gel with a nylon mesh. Expanding duct epithelium can be seen in the lattice. Original magnification, ×100. C: Electron microscopy of the cultured pancreatic duct cells. Their epithelial nature was apparent by the prominent microvillus processes along portions of the plasma membrane ( thick white arrows ) and formed tight junctions ( solid black arrows ) between the cohered cells. No secretary granules are seen to suggest acinar cell derivation.
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    Millipore 108 collagenase d
    Enriched pancreatic ducts and pancreatic duct epithelium in culture. A: Enriched pancreatic ducts after partial digestion with <t>collagenase</t> D and filtration through 85-μm nylon mesh. Phase contrast, original magnification, ×100. B: Primary culture of duct cells embedded in rat-tail collagen gel with a nylon mesh. Expanding duct epithelium can be seen in the lattice. Original magnification, ×100. C: Electron microscopy of the cultured pancreatic duct cells. Their epithelial nature was apparent by the prominent microvillus processes along portions of the plasma membrane ( thick white arrows ) and formed tight junctions ( solid black arrows ) between the cohered cells. No secretary granules are seen to suggest acinar cell derivation.
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    Thermo Fisher collagenase type d
    Enriched pancreatic ducts and pancreatic duct epithelium in culture. A: Enriched pancreatic ducts after partial digestion with <t>collagenase</t> D and filtration through 85-μm nylon mesh. Phase contrast, original magnification, ×100. B: Primary culture of duct cells embedded in rat-tail collagen gel with a nylon mesh. Expanding duct epithelium can be seen in the lattice. Original magnification, ×100. C: Electron microscopy of the cultured pancreatic duct cells. Their epithelial nature was apparent by the prominent microvillus processes along portions of the plasma membrane ( thick white arrows ) and formed tight junctions ( solid black arrows ) between the cohered cells. No secretary granules are seen to suggest acinar cell derivation.
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    Boehringer Mannheim collagenase a collagenase d
    Enriched pancreatic ducts and pancreatic duct epithelium in culture. A: Enriched pancreatic ducts after partial digestion with <t>collagenase</t> D and filtration through 85-μm nylon mesh. Phase contrast, original magnification, ×100. B: Primary culture of duct cells embedded in rat-tail collagen gel with a nylon mesh. Expanding duct epithelium can be seen in the lattice. Original magnification, ×100. C: Electron microscopy of the cultured pancreatic duct cells. Their epithelial nature was apparent by the prominent microvillus processes along portions of the plasma membrane ( thick white arrows ) and formed tight junctions ( solid black arrows ) between the cohered cells. No secretary granules are seen to suggest acinar cell derivation.
    Collagenase A Collagenase D, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 80/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Cetuximab modulates macrophage polarization in an AOM/DSS mouse model A. Establishment of the AOM/DSS mouse model. AOM was injected intraperitoneally at 12.5 mg/kg body weight. After one week, mice were given drinking water containing 2.5% DSS for 5 days followed by 16 days of regular drinking water. After two cycles of DSS treatment, cetuximab (1 mg/mouse, twice a week) was injected intraperitoneally for a month, and the mice were then sacrificed. B. Representative images of colon tumors in normal (right), AOM/DSS (2AD) (middle), and cetuximab-treated AOM/DSS mice (2AD+cetu) (left). C. Tumor quantification. Cetuximab treatment (2AD + cetu) reduced tumor numbers compared to 2AD mice. D. p-EGFR (Y1068), EGFR, Arg1, and iNOS protein levels in normal mice, 2AD and 2AD+cetu mouse tumors were detected by Western blot. All experiments were repeated three times. E. Representative photomicrographs of immunostaining for p-EGFR (Y1068), PCNA, F4/80, Arg1, and iNOS. Scale bars: 25 μm. F. M1 marker (IL-12, iNOS) and M2 marker (IL-4, IL-10, Arg1) mRNA levels in normal mouse colon tissues, 2AD and 2AD+cetu mouse tumor tissues were evaluated by q-PCR. G. Percentages of CD11b + /F4/80 + and F4/80 + /CD206 + cells in normal, 2AD, and 2AD+cetu mice colon tissues were detected by flow cytometry. Colon tissues were cut into small pieces (1-2 mm) and incubated with collagenase D (1- mg/mL), dispase II (1 mg/mL), and DNase I (100 μg/mL) for 30-45 min in a shaking incubator at 37°C, and single-cell suspensions were then incubated with antibodies. Bars represent means ± SD (n = 3) for each treatment. * p

    Journal: Oncotarget

    Article Title: Polarization of macrophages in the tumor microenvironment is influenced by EGFR signaling within colon cancer cells

    doi: 10.18632/oncotarget.12207

    Figure Lengend Snippet: Cetuximab modulates macrophage polarization in an AOM/DSS mouse model A. Establishment of the AOM/DSS mouse model. AOM was injected intraperitoneally at 12.5 mg/kg body weight. After one week, mice were given drinking water containing 2.5% DSS for 5 days followed by 16 days of regular drinking water. After two cycles of DSS treatment, cetuximab (1 mg/mouse, twice a week) was injected intraperitoneally for a month, and the mice were then sacrificed. B. Representative images of colon tumors in normal (right), AOM/DSS (2AD) (middle), and cetuximab-treated AOM/DSS mice (2AD+cetu) (left). C. Tumor quantification. Cetuximab treatment (2AD + cetu) reduced tumor numbers compared to 2AD mice. D. p-EGFR (Y1068), EGFR, Arg1, and iNOS protein levels in normal mice, 2AD and 2AD+cetu mouse tumors were detected by Western blot. All experiments were repeated three times. E. Representative photomicrographs of immunostaining for p-EGFR (Y1068), PCNA, F4/80, Arg1, and iNOS. Scale bars: 25 μm. F. M1 marker (IL-12, iNOS) and M2 marker (IL-4, IL-10, Arg1) mRNA levels in normal mouse colon tissues, 2AD and 2AD+cetu mouse tumor tissues were evaluated by q-PCR. G. Percentages of CD11b + /F4/80 + and F4/80 + /CD206 + cells in normal, 2AD, and 2AD+cetu mice colon tissues were detected by flow cytometry. Colon tissues were cut into small pieces (1-2 mm) and incubated with collagenase D (1- mg/mL), dispase II (1 mg/mL), and DNase I (100 μg/mL) for 30-45 min in a shaking incubator at 37°C, and single-cell suspensions were then incubated with antibodies. Bars represent means ± SD (n = 3) for each treatment. * p

    Article Snippet: Briefly, colon tissues were cut into small pieces (1-2 mm) and incubated in 10 mL PRMI 1640 medium with 10 mM HEPES and 5% fetal bovine serum containing 10 mg (1 mg/mL) collagenase D (Sigma-Aldrich), 10 mg (1 mg/mL) dispase II (Roche, Germany), and 100 μL 10 mg/ml DNase I (100 μg/mL) (Sigma-Aldrich) for 30-45 min in a shaking incubator at 37°C.

    Techniques: Injection, Mouse Assay, Western Blot, Immunostaining, Marker, Polymerase Chain Reaction, Flow Cytometry, Cytometry, Incubation

    Enriched pancreatic ducts and pancreatic duct epithelium in culture. A: Enriched pancreatic ducts after partial digestion with collagenase D and filtration through 85-μm nylon mesh. Phase contrast, original magnification, ×100. B: Primary culture of duct cells embedded in rat-tail collagen gel with a nylon mesh. Expanding duct epithelium can be seen in the lattice. Original magnification, ×100. C: Electron microscopy of the cultured pancreatic duct cells. Their epithelial nature was apparent by the prominent microvillus processes along portions of the plasma membrane ( thick white arrows ) and formed tight junctions ( solid black arrows ) between the cohered cells. No secretary granules are seen to suggest acinar cell derivation.

    Journal: The American Journal of Pathology

    Article Title: Liver Repopulation and Correction of Metabolic Liver Disease by Transplanted Adult Mouse Pancreatic Cells

    doi:

    Figure Lengend Snippet: Enriched pancreatic ducts and pancreatic duct epithelium in culture. A: Enriched pancreatic ducts after partial digestion with collagenase D and filtration through 85-μm nylon mesh. Phase contrast, original magnification, ×100. B: Primary culture of duct cells embedded in rat-tail collagen gel with a nylon mesh. Expanding duct epithelium can be seen in the lattice. Original magnification, ×100. C: Electron microscopy of the cultured pancreatic duct cells. Their epithelial nature was apparent by the prominent microvillus processes along portions of the plasma membrane ( thick white arrows ) and formed tight junctions ( solid black arrows ) between the cohered cells. No secretary granules are seen to suggest acinar cell derivation.

    Article Snippet: The harvested pancreas was immediately cut into small pieces and digested by collagenase D (Roche, Indianapolis, IN) (2.5 mg/ml, dissolved in Earle’s basic salt solution) for 25 minutes at 37°C with agitation by a magnetic stir bar.

    Techniques: Filtration, Electron Microscopy, Cell Culture