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  • 96
    Carna Inc homo sapiens sik1
    Potency and Selectivity of SIK Inhibitors
    Homo Sapiens Sik1, supplied by Carna Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/homo sapiens sik1/product/Carna Inc
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    homo sapiens sik1 - by Bioz Stars, 2024-06
    96/100 stars
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    93
    CLS Cell Lines Service GmbH u 2 os cells
    Mic60 is a component of the MICOS complex, and is involved in the formation and maintenance of crista junctions that connect the crista membrane with the inner boundary membrane. a , Mic60 in a <t>U-2</t> OS cell, labeled with primary and secondary antibodies. The Mic60 signals appear as structured, punctate clusters. The localizations are color coded according to their z coordinate (identical color scales in a – d ). Scale bar, 200 nm. b , Magnified view of the boxed region in a . Scale bar, 50 nm. c , Mic60 in a COS-7 cell, in which the crista junctions exhibit a linear organization over segments of the inner boundary membrane. Scale bar, 200 nm. d , Magnified view of the boxed region in c . Scale bar, 50 nm. e , f , Unwrapped views of the Mic60 localization density around the surface of the mitochondria, showing the nanoscale distribution of Mic60. In U-2 OS cells, Mic60 appears predominantly punctate, with pairs or clusters of signal density separated by 20–40 nm (Extended Data Fig. and Supplementary Fig. ). In COS-7 cells, Mic60 appears to have a zigzag or double-line arrangement, with a typical width of approximately 25 nm (Extended Data Fig. and Supplementary Fig. ). Dashed lines indicate the extent of the data in f . g , Two-color image of Mic60 (blue) and mitochondrial nucleoids (yellow) in a COS-7 cell, stained with antibodies labeled with Alexa Fluor 647 and Cy5.5, respectively. Scale bar, 1 µm. h , Detailed view of the boxed region in g . Lower density of Mic60 close to the DNA signal, suggesting fewer crista junctions in these regions. i , Cross-section ( x – z ) through the region indicated by the dashed lines in h , showing Mic60 at the inner boundary membrane, and a DNA cluster in the center of the mitochondrion. j , A 3D perspective view of the mitochondrion shown in h and i , where the Mic60 and DNA signals have been rendered as isosurfaces. Scale bars, 250 nm ( h – j ).
    U 2 Os Cells, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/u 2 os cells/product/CLS Cell Lines Service GmbH
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    u 2 os cells - by Bioz Stars, 2024-06
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    93
    CLS Cell Lines Service GmbH nup96 snap tag cells
    a Fusion of 300 STORM <t>Nup96</t> particles and the estimated rotational symmetry axis. b Histogram of the trace( S ij ) reveals the 8-fold rotational symmetry of the Nup96 protein. c Density plot of the estimated axes of rotation on the unit sphere for the Nup96 dataset. d Fusion of 400 3D PAINT DNA-origami tetrahedron particles and the estimated axes of rotation. The white and cyan bars indicate the 3-fold and 2-fold rotational symmetry axes, respectively. e Histogram of the trace( S ij ) reveals the 3- and 2-fold rotational symmetries of the tetrahedron structure. f Density plot of the estimated axes of rotation on the unit sphere. The dense regions on the unit sphere in c , f project the orientation of the estimated axis(es) of rotations.
    Nup96 Snap Tag Cells, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nup96 snap tag cells/product/CLS Cell Lines Service GmbH
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nup96 snap tag cells - by Bioz Stars, 2024-06
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    92
    CLS Cell Lines Service GmbH a498 cells
    a Fusion of 300 STORM <t>Nup96</t> particles and the estimated rotational symmetry axis. b Histogram of the trace( S ij ) reveals the 8-fold rotational symmetry of the Nup96 protein. c Density plot of the estimated axes of rotation on the unit sphere for the Nup96 dataset. d Fusion of 400 3D PAINT DNA-origami tetrahedron particles and the estimated axes of rotation. The white and cyan bars indicate the 3-fold and 2-fold rotational symmetry axes, respectively. e Histogram of the trace( S ij ) reveals the 3- and 2-fold rotational symmetries of the tetrahedron structure. f Density plot of the estimated axes of rotation on the unit sphere. The dense regions on the unit sphere in c , f project the orientation of the estimated axis(es) of rotations.
    A498 Cells, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/a498 cells/product/CLS Cell Lines Service GmbH
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    a498 cells - by Bioz Stars, 2024-06
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    93
    CLS Cell Lines Service GmbH syngeneic colon adenocarcinoma cc531 cells
    a Fusion of 300 STORM <t>Nup96</t> particles and the estimated rotational symmetry axis. b Histogram of the trace( S ij ) reveals the 8-fold rotational symmetry of the Nup96 protein. c Density plot of the estimated axes of rotation on the unit sphere for the Nup96 dataset. d Fusion of 400 3D PAINT DNA-origami tetrahedron particles and the estimated axes of rotation. The white and cyan bars indicate the 3-fold and 2-fold rotational symmetry axes, respectively. e Histogram of the trace( S ij ) reveals the 3- and 2-fold rotational symmetries of the tetrahedron structure. f Density plot of the estimated axes of rotation on the unit sphere. The dense regions on the unit sphere in c , f project the orientation of the estimated axis(es) of rotations.
    Syngeneic Colon Adenocarcinoma Cc531 Cells, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/syngeneic colon adenocarcinoma cc531 cells/product/CLS Cell Lines Service GmbH
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    syngeneic colon adenocarcinoma cc531 cells - by Bioz Stars, 2024-06
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    Image Search Results


    Potency and Selectivity of SIK Inhibitors

    Journal: Journal of Medicinal Chemistry

    Article Title: Optimization of Selectivity and Pharmacokinetic Properties of Salt-Inducible Kinase Inhibitors that Led to the Discovery of Pan-SIK Inhibitor GLPG3312

    doi: 10.1021/acs.jmedchem.3c01428

    Figure Lengend Snippet: Potency and Selectivity of SIK Inhibitors

    Article Snippet: Homo sapiens SIK1 (full length, reference 02-131), ALK5 (catalytic domain aa200-503, reference 09-141), AMPKα1/β2/γ1 (full length, reference 02-147), LynA (full length, reference 08-171), and TGFβR2 (catalytic domain aa194-567, reference 09-142) were purchased from Carna Biosciences, DE.

    Techniques: Inhibition, Binding Assay

    Exploration of Carboxamide Alkylation

    Journal: Journal of Medicinal Chemistry

    Article Title: Optimization of Selectivity and Pharmacokinetic Properties of Salt-Inducible Kinase Inhibitors that Led to the Discovery of Pan-SIK Inhibitor GLPG3312

    doi: 10.1021/acs.jmedchem.3c01428

    Figure Lengend Snippet: Exploration of Carboxamide Alkylation

    Article Snippet: Homo sapiens SIK1 (full length, reference 02-131), ALK5 (catalytic domain aa200-503, reference 09-141), AMPKα1/β2/γ1 (full length, reference 02-147), LynA (full length, reference 08-171), and TGFβR2 (catalytic domain aa194-567, reference 09-142) were purchased from Carna Biosciences, DE.

    Techniques:

    Pharmacokinetics in Mice and the Structure–Property Relationship

    Journal: Journal of Medicinal Chemistry

    Article Title: Optimization of Selectivity and Pharmacokinetic Properties of Salt-Inducible Kinase Inhibitors that Led to the Discovery of Pan-SIK Inhibitor GLPG3312

    doi: 10.1021/acs.jmedchem.3c01428

    Figure Lengend Snippet: Pharmacokinetics in Mice and the Structure–Property Relationship

    Article Snippet: Homo sapiens SIK1 (full length, reference 02-131), ALK5 (catalytic domain aa200-503, reference 09-141), AMPKα1/β2/γ1 (full length, reference 02-147), LynA (full length, reference 08-171), and TGFβR2 (catalytic domain aa194-567, reference 09-142) were purchased from Carna Biosciences, DE.

    Techniques:

    Potency of 28 on Off-Targets from a 380 Kinase Panel Inhibited by 50% or More at 1 μM of 28 and Fold Shift versus Potency on  SIK1,  SIK2, and SIK3

    Journal: Journal of Medicinal Chemistry

    Article Title: Optimization of Selectivity and Pharmacokinetic Properties of Salt-Inducible Kinase Inhibitors that Led to the Discovery of Pan-SIK Inhibitor GLPG3312

    doi: 10.1021/acs.jmedchem.3c01428

    Figure Lengend Snippet: Potency of 28 on Off-Targets from a 380 Kinase Panel Inhibited by 50% or More at 1 μM of 28 and Fold Shift versus Potency on SIK1, SIK2, and SIK3

    Article Snippet: Homo sapiens SIK1 (full length, reference 02-131), ALK5 (catalytic domain aa200-503, reference 09-141), AMPKα1/β2/γ1 (full length, reference 02-147), LynA (full length, reference 08-171), and TGFβR2 (catalytic domain aa194-567, reference 09-142) were purchased from Carna Biosciences, DE.

    Techniques:

    Mic60 is a component of the MICOS complex, and is involved in the formation and maintenance of crista junctions that connect the crista membrane with the inner boundary membrane. a , Mic60 in a U-2 OS cell, labeled with primary and secondary antibodies. The Mic60 signals appear as structured, punctate clusters. The localizations are color coded according to their z coordinate (identical color scales in a – d ). Scale bar, 200 nm. b , Magnified view of the boxed region in a . Scale bar, 50 nm. c , Mic60 in a COS-7 cell, in which the crista junctions exhibit a linear organization over segments of the inner boundary membrane. Scale bar, 200 nm. d , Magnified view of the boxed region in c . Scale bar, 50 nm. e , f , Unwrapped views of the Mic60 localization density around the surface of the mitochondria, showing the nanoscale distribution of Mic60. In U-2 OS cells, Mic60 appears predominantly punctate, with pairs or clusters of signal density separated by 20–40 nm (Extended Data Fig. and Supplementary Fig. ). In COS-7 cells, Mic60 appears to have a zigzag or double-line arrangement, with a typical width of approximately 25 nm (Extended Data Fig. and Supplementary Fig. ). Dashed lines indicate the extent of the data in f . g , Two-color image of Mic60 (blue) and mitochondrial nucleoids (yellow) in a COS-7 cell, stained with antibodies labeled with Alexa Fluor 647 and Cy5.5, respectively. Scale bar, 1 µm. h , Detailed view of the boxed region in g . Lower density of Mic60 close to the DNA signal, suggesting fewer crista junctions in these regions. i , Cross-section ( x – z ) through the region indicated by the dashed lines in h , showing Mic60 at the inner boundary membrane, and a DNA cluster in the center of the mitochondrion. j , A 3D perspective view of the mitochondrion shown in h and i , where the Mic60 and DNA signals have been rendered as isosurfaces. Scale bars, 250 nm ( h – j ).

    Journal: Nature Methods

    Article Title: Optimal precision and accuracy in 4Pi-STORM using dynamic spline PSF models

    doi: 10.1038/s41592-022-01465-8

    Figure Lengend Snippet: Mic60 is a component of the MICOS complex, and is involved in the formation and maintenance of crista junctions that connect the crista membrane with the inner boundary membrane. a , Mic60 in a U-2 OS cell, labeled with primary and secondary antibodies. The Mic60 signals appear as structured, punctate clusters. The localizations are color coded according to their z coordinate (identical color scales in a – d ). Scale bar, 200 nm. b , Magnified view of the boxed region in a . Scale bar, 50 nm. c , Mic60 in a COS-7 cell, in which the crista junctions exhibit a linear organization over segments of the inner boundary membrane. Scale bar, 200 nm. d , Magnified view of the boxed region in c . Scale bar, 50 nm. e , f , Unwrapped views of the Mic60 localization density around the surface of the mitochondria, showing the nanoscale distribution of Mic60. In U-2 OS cells, Mic60 appears predominantly punctate, with pairs or clusters of signal density separated by 20–40 nm (Extended Data Fig. and Supplementary Fig. ). In COS-7 cells, Mic60 appears to have a zigzag or double-line arrangement, with a typical width of approximately 25 nm (Extended Data Fig. and Supplementary Fig. ). Dashed lines indicate the extent of the data in f . g , Two-color image of Mic60 (blue) and mitochondrial nucleoids (yellow) in a COS-7 cell, stained with antibodies labeled with Alexa Fluor 647 and Cy5.5, respectively. Scale bar, 1 µm. h , Detailed view of the boxed region in g . Lower density of Mic60 close to the DNA signal, suggesting fewer crista junctions in these regions. i , Cross-section ( x – z ) through the region indicated by the dashed lines in h , showing Mic60 at the inner boundary membrane, and a DNA cluster in the center of the mitochondrion. j , A 3D perspective view of the mitochondrion shown in h and i , where the Mic60 and DNA signals have been rendered as isosurfaces. Scale bars, 250 nm ( h – j ).

    Article Snippet: Experiments were performed using either standard COS-7 cells or U-2 OS cells obtained from American Type Culture Collection (ATCC), or gene-edited U-2 OS cells expressing a SNAP-tagged version of the nucleoporin Nup107 (CLS Cell Lines Service, U-2OS-ZFN-SNAP-Nup107 clone 294) or Nup96 (CLS Cell Lines Service, U-2OS-CRISPR-NUP96-SNAP clone 33) .

    Techniques: Labeling, Staining

    a Fusion of 300 STORM Nup96 particles and the estimated rotational symmetry axis. b Histogram of the trace( S ij ) reveals the 8-fold rotational symmetry of the Nup96 protein. c Density plot of the estimated axes of rotation on the unit sphere for the Nup96 dataset. d Fusion of 400 3D PAINT DNA-origami tetrahedron particles and the estimated axes of rotation. The white and cyan bars indicate the 3-fold and 2-fold rotational symmetry axes, respectively. e Histogram of the trace( S ij ) reveals the 3- and 2-fold rotational symmetries of the tetrahedron structure. f Density plot of the estimated axes of rotation on the unit sphere. The dense regions on the unit sphere in c , f project the orientation of the estimated axis(es) of rotations.

    Journal: Nature Communications

    Article Title: 3D particle averaging and detection of macromolecular symmetry in localization microscopy

    doi: 10.1038/s41467-021-22006-5

    Figure Lengend Snippet: a Fusion of 300 STORM Nup96 particles and the estimated rotational symmetry axis. b Histogram of the trace( S ij ) reveals the 8-fold rotational symmetry of the Nup96 protein. c Density plot of the estimated axes of rotation on the unit sphere for the Nup96 dataset. d Fusion of 400 3D PAINT DNA-origami tetrahedron particles and the estimated axes of rotation. The white and cyan bars indicate the 3-fold and 2-fold rotational symmetry axes, respectively. e Histogram of the trace( S ij ) reveals the 3- and 2-fold rotational symmetries of the tetrahedron structure. f Density plot of the estimated axes of rotation on the unit sphere. The dense regions on the unit sphere in c , f project the orientation of the estimated axis(es) of rotations.

    Article Snippet: Rinse 2x Coverslips containing Nup96-SNAP-tag cells (catalog no. 300444,CLS Cell Line Service) with warm PBS.

    Techniques: