click-it rna alexa fluor 488 imaging kit Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher click it rna alexa fluor 488 imaging kit
    EVs contain plasmodial RNAs. ( a ) Quantification of P. falciparum RNAs transferred to EVs. Infected RBCs were labeled with Click-It <t>RNA</t> <t>Alexa</t> Fluor 488. After 30 h. of incubation, EVs were collected and EU incorporation was analyzed by Flow Cytometry. ( b ) Distribution of the reads mapped to the P. falciparum genome according to the corresponding chromosomes. ( c ) Distribution of the reads mapped to the P. falciparum according to the size of the chromosome. ( d ) Types of Plasmodium genes in the EVs by RNA-Seq. ( e ) Extracellular vesicles contain a large number of Apicoplast tRNA and Mitochondrial rRNA. ( f ) RNA expression is different in EVs than mixed stage parasite expression pattern. ( g ) EV RNA composition is different that of iRBC. The expression levels shown by RNAseq of EV has no detectable correlation with that of steady state iRBC, showing that EV is not merely a smaller form of iRBC. Neither parametric (Pearson’s R) nor parametric correlations (Kendell’s tau) analysis detected correlation between EV and iRBC at any time points with correlation larger than 0.001. The X-axis shows the average steady state RNA expression levels.
    Click It Rna Alexa Fluor 488 Imaging Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1925 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/click it rna alexa fluor 488 imaging kit/product/Thermo Fisher
    Average 99 stars, based on 1925 article reviews
    Price from $9.99 to $1999.99
    click it rna alexa fluor 488 imaging kit - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    95
    Thermo Fisher click it rna alexa fluor 488 hcs assay
    Protein stability and cell cycle measurements in mouse and human neural progenitors. (A) RT-qPCR analysis of Pax6, Olig2, Nkx2.2 and Isl1 expression in mouse and human differentiations from the Ptchl-mKATE2 targeted stem cell lines. (B) Representative histograms of mKATE2 intensity measurements to estimate protein half-life from mouse (orange) and human (blue) neural progenitors. (C) Representatve histograms of DNA content of mouse and human cells, and dual parameter plots of <t>Alexa</t> Fluor 488-conjugated EdU and DNA indicating the gates to quantify EdU positive cells. (D) Pairwise relationships between proportion of S phase estimations (c 0 ), cell cycle lenght ( T ) and saturation (σ) in mouse and human cells.
    Click It Rna Alexa Fluor 488 Hcs Assay, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/click it rna alexa fluor 488 hcs assay/product/Thermo Fisher
    Average 95 stars, based on 21 article reviews
    Price from $9.99 to $1999.99
    click it rna alexa fluor 488 hcs assay - by Bioz Stars, 2020-09
    95/100 stars
      Buy from Supplier

    99
    Thermo Fisher click it rna alexa fluor 594 imaging kit
    Protein stability and cell cycle measurements in mouse and human neural progenitors. (A) RT-qPCR analysis of Pax6, Olig2, Nkx2.2 and Isl1 expression in mouse and human differentiations from the Ptchl-mKATE2 targeted stem cell lines. (B) Representative histograms of mKATE2 intensity measurements to estimate protein half-life from mouse (orange) and human (blue) neural progenitors. (C) Representatve histograms of DNA content of mouse and human cells, and dual parameter plots of <t>Alexa</t> Fluor 488-conjugated EdU and DNA indicating the gates to quantify EdU positive cells. (D) Pairwise relationships between proportion of S phase estimations (c 0 ), cell cycle lenght ( T ) and saturation (σ) in mouse and human cells.
    Click It Rna Alexa Fluor 594 Imaging Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 202 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/click it rna alexa fluor 594 imaging kit/product/Thermo Fisher
    Average 99 stars, based on 202 article reviews
    Price from $9.99 to $1999.99
    click it rna alexa fluor 594 imaging kit - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher click it rna alexa fluor 488 imaging kit thermo fisher scientific
    Protein stability and cell cycle measurements in mouse and human neural progenitors. (A) RT-qPCR analysis of Pax6, Olig2, Nkx2.2 and Isl1 expression in mouse and human differentiations from the Ptchl-mKATE2 targeted stem cell lines. (B) Representative histograms of mKATE2 intensity measurements to estimate protein half-life from mouse (orange) and human (blue) neural progenitors. (C) Representatve histograms of DNA content of mouse and human cells, and dual parameter plots of <t>Alexa</t> Fluor 488-conjugated EdU and DNA indicating the gates to quantify EdU positive cells. (D) Pairwise relationships between proportion of S phase estimations (c 0 ), cell cycle lenght ( T ) and saturation (σ) in mouse and human cells.
    Click It Rna Alexa Fluor 488 Imaging Kit Thermo Fisher Scientific, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 718 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/click it rna alexa fluor 488 imaging kit thermo fisher scientific/product/Thermo Fisher
    Average 99 stars, based on 718 article reviews
    Price from $9.99 to $1999.99
    click it rna alexa fluor 488 imaging kit thermo fisher scientific - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher click it plus edu flow cytometry assay kit alexa fluor 488 picolyl azide
    Protein stability and cell cycle measurements in mouse and human neural progenitors. (A) RT-qPCR analysis of Pax6, Olig2, Nkx2.2 and Isl1 expression in mouse and human differentiations from the Ptchl-mKATE2 targeted stem cell lines. (B) Representative histograms of mKATE2 intensity measurements to estimate protein half-life from mouse (orange) and human (blue) neural progenitors. (C) Representatve histograms of DNA content of mouse and human cells, and dual parameter plots of <t>Alexa</t> Fluor 488-conjugated EdU and DNA indicating the gates to quantify EdU positive cells. (D) Pairwise relationships between proportion of S phase estimations (c 0 ), cell cycle lenght ( T ) and saturation (σ) in mouse and human cells.
    Click It Plus Edu Flow Cytometry Assay Kit Alexa Fluor 488 Picolyl Azide, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/click it plus edu flow cytometry assay kit alexa fluor 488 picolyl azide/product/Thermo Fisher
    Average 99 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    click it plus edu flow cytometry assay kit alexa fluor 488 picolyl azide - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher click it edu alexa fluor 488 kit
    ASK induces inhibition of proliferation in HeLa cells. a , differential expression of the SncmtRNA ( S; green ) and ASncmtRNAs ( AS; red ) in human keratinocytes and HeLa cells as observed by FISH. b , schematic representation of the AS-1 and -2, indicating the relative position of the ASOs utilized in this work. c , transfection of different tumor cell lines with fluorescently labeled ASO-1537S. The indicated cell lines were seeded at 5 × 10 4 cells/well into 12-well plates and transfected the next day with ASO-1537S coupled to <t>Alexa</t> <t>Fluor</t> 488 (see Table 1 ). At 24 h post-transfection > 90% of cells were fluorescently labeled ( left panel , fluorescence; right panel , phase contrast. Bars = 20 μm). d , proliferation of HeLa cells. Cells (5 × 10 4 cells/well) were seeded into 12-well plates and transfected the next day with ASO-C or ASO-1537S (see “Experimental Procedures”) or left untreated ( NT ). At the indicated times cells were harvested and counted. At 48 h post-transfection in triplicate, ASK with ASO-1537S induces significant inhibition of cell proliferation as compared with controls (data are presented as the mean ± S.E.; * p
    Click It Edu Alexa Fluor 488 Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 197 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/click it edu alexa fluor 488 kit/product/Thermo Fisher
    Average 99 stars, based on 197 article reviews
    Price from $9.99 to $1999.99
    click it edu alexa fluor 488 kit - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    EVs contain plasmodial RNAs. ( a ) Quantification of P. falciparum RNAs transferred to EVs. Infected RBCs were labeled with Click-It RNA Alexa Fluor 488. After 30 h. of incubation, EVs were collected and EU incorporation was analyzed by Flow Cytometry. ( b ) Distribution of the reads mapped to the P. falciparum genome according to the corresponding chromosomes. ( c ) Distribution of the reads mapped to the P. falciparum according to the size of the chromosome. ( d ) Types of Plasmodium genes in the EVs by RNA-Seq. ( e ) Extracellular vesicles contain a large number of Apicoplast tRNA and Mitochondrial rRNA. ( f ) RNA expression is different in EVs than mixed stage parasite expression pattern. ( g ) EV RNA composition is different that of iRBC. The expression levels shown by RNAseq of EV has no detectable correlation with that of steady state iRBC, showing that EV is not merely a smaller form of iRBC. Neither parametric (Pearson’s R) nor parametric correlations (Kendell’s tau) analysis detected correlation between EV and iRBC at any time points with correlation larger than 0.001. The X-axis shows the average steady state RNA expression levels.

    Journal: Scientific Reports

    Article Title: Malaria infected red blood cells release small regulatory RNAs through extracellular vesicles

    doi: 10.1038/s41598-018-19149-9

    Figure Lengend Snippet: EVs contain plasmodial RNAs. ( a ) Quantification of P. falciparum RNAs transferred to EVs. Infected RBCs were labeled with Click-It RNA Alexa Fluor 488. After 30 h. of incubation, EVs were collected and EU incorporation was analyzed by Flow Cytometry. ( b ) Distribution of the reads mapped to the P. falciparum genome according to the corresponding chromosomes. ( c ) Distribution of the reads mapped to the P. falciparum according to the size of the chromosome. ( d ) Types of Plasmodium genes in the EVs by RNA-Seq. ( e ) Extracellular vesicles contain a large number of Apicoplast tRNA and Mitochondrial rRNA. ( f ) RNA expression is different in EVs than mixed stage parasite expression pattern. ( g ) EV RNA composition is different that of iRBC. The expression levels shown by RNAseq of EV has no detectable correlation with that of steady state iRBC, showing that EV is not merely a smaller form of iRBC. Neither parametric (Pearson’s R) nor parametric correlations (Kendell’s tau) analysis detected correlation between EV and iRBC at any time points with correlation larger than 0.001. The X-axis shows the average steady state RNA expression levels.

    Article Snippet: EU incorporated EV RNA was detected using Click chemistry according to the manufacturer’s protocol (Invitrogen, C10329) and nuclei were counterstained using Hoechst 33342 and analyzed with a MACSQuant VYB flow cytometer.

    Techniques: Infection, Labeling, Incubation, Flow Cytometry, Cytometry, RNA Sequencing Assay, RNA Expression, Expressing

    Detection of transcription in the nuclei of P. sativum var. Paloma suspensor. a, c, e DNA stained with DAPI in the polyploid nuclei ( a ), in the polyploid nucleus during the amitotic division ( c ), in the nuclei during classical cell cycle ( e ); mc—metaphase chromosomes. b, d, f The same nuclei as previously after 5-ethynyl uridine – (EU) incorporation stained with Click-iT ® RNA Alexa Fluor ® 488. Scale bar a – d − 50 µm; e, f − 10 µm

    Journal: Plant Cell Reports

    Article Title: Endoreplication and its consequences in the suspensor of Pisum sativum

    doi: 10.1007/s00299-018-2335-0

    Figure Lengend Snippet: Detection of transcription in the nuclei of P. sativum var. Paloma suspensor. a, c, e DNA stained with DAPI in the polyploid nuclei ( a ), in the polyploid nucleus during the amitotic division ( c ), in the nuclei during classical cell cycle ( e ); mc—metaphase chromosomes. b, d, f The same nuclei as previously after 5-ethynyl uridine – (EU) incorporation stained with Click-iT ® RNA Alexa Fluor ® 488. Scale bar a – d − 50 µm; e, f − 10 µm

    Article Snippet: Labeling and detection of RNA transcripts Click-iT® RNA Alexa Fluor® 488 Imaging Kit (Invitrogen) was used for the visualization of RNA transcripts (Jao and Salic ).

    Techniques: Staining

    Protein stability and cell cycle measurements in mouse and human neural progenitors. (A) RT-qPCR analysis of Pax6, Olig2, Nkx2.2 and Isl1 expression in mouse and human differentiations from the Ptchl-mKATE2 targeted stem cell lines. (B) Representative histograms of mKATE2 intensity measurements to estimate protein half-life from mouse (orange) and human (blue) neural progenitors. (C) Representatve histograms of DNA content of mouse and human cells, and dual parameter plots of Alexa Fluor 488-conjugated EdU and DNA indicating the gates to quantify EdU positive cells. (D) Pairwise relationships between proportion of S phase estimations (c 0 ), cell cycle lenght ( T ) and saturation (σ) in mouse and human cells.

    Journal: bioRxiv

    Article Title: Species-specific developmental timing is associated with global differences in protein stability in mouse and human

    doi: 10.1101/2019.12.29.889543

    Figure Lengend Snippet: Protein stability and cell cycle measurements in mouse and human neural progenitors. (A) RT-qPCR analysis of Pax6, Olig2, Nkx2.2 and Isl1 expression in mouse and human differentiations from the Ptchl-mKATE2 targeted stem cell lines. (B) Representative histograms of mKATE2 intensity measurements to estimate protein half-life from mouse (orange) and human (blue) neural progenitors. (C) Representatve histograms of DNA content of mouse and human cells, and dual parameter plots of Alexa Fluor 488-conjugated EdU and DNA indicating the gates to quantify EdU positive cells. (D) Pairwise relationships between proportion of S phase estimations (c 0 ), cell cycle lenght ( T ) and saturation (σ) in mouse and human cells.

    Article Snippet: EU-incorporated RNAs were labelled using Click-iT™ RNA Alexa Fluor 488 HCS Assay (Thermo Fisher Scientific C10327), and AHA-incorporated proteins were labeled using Click-iT™ Cell Reaction Buffer Kit (Thermo Fisher Scientific C10269) on a volume of 150ul per sample reaction.

    Techniques: Quantitative RT-PCR, Expressing

    ASK induces inhibition of proliferation in HeLa cells. a , differential expression of the SncmtRNA ( S; green ) and ASncmtRNAs ( AS; red ) in human keratinocytes and HeLa cells as observed by FISH. b , schematic representation of the AS-1 and -2, indicating the relative position of the ASOs utilized in this work. c , transfection of different tumor cell lines with fluorescently labeled ASO-1537S. The indicated cell lines were seeded at 5 × 10 4 cells/well into 12-well plates and transfected the next day with ASO-1537S coupled to Alexa Fluor 488 (see Table 1 ). At 24 h post-transfection > 90% of cells were fluorescently labeled ( left panel , fluorescence; right panel , phase contrast. Bars = 20 μm). d , proliferation of HeLa cells. Cells (5 × 10 4 cells/well) were seeded into 12-well plates and transfected the next day with ASO-C or ASO-1537S (see “Experimental Procedures”) or left untreated ( NT ). At the indicated times cells were harvested and counted. At 48 h post-transfection in triplicate, ASK with ASO-1537S induces significant inhibition of cell proliferation as compared with controls (data are presented as the mean ± S.E.; * p

    Journal: The Journal of Biological Chemistry

    Article Title: Down-regulation of the Antisense Mitochondrial Non-coding RNAs (ncRNAs) Is a Unique Vulnerability of Cancer Cells and a Potential Target for Cancer Therapy *

    doi: 10.1074/jbc.M114.558841

    Figure Lengend Snippet: ASK induces inhibition of proliferation in HeLa cells. a , differential expression of the SncmtRNA ( S; green ) and ASncmtRNAs ( AS; red ) in human keratinocytes and HeLa cells as observed by FISH. b , schematic representation of the AS-1 and -2, indicating the relative position of the ASOs utilized in this work. c , transfection of different tumor cell lines with fluorescently labeled ASO-1537S. The indicated cell lines were seeded at 5 × 10 4 cells/well into 12-well plates and transfected the next day with ASO-1537S coupled to Alexa Fluor 488 (see Table 1 ). At 24 h post-transfection > 90% of cells were fluorescently labeled ( left panel , fluorescence; right panel , phase contrast. Bars = 20 μm). d , proliferation of HeLa cells. Cells (5 × 10 4 cells/well) were seeded into 12-well plates and transfected the next day with ASO-C or ASO-1537S (see “Experimental Procedures”) or left untreated ( NT ). At the indicated times cells were harvested and counted. At 48 h post-transfection in triplicate, ASK with ASO-1537S induces significant inhibition of cell proliferation as compared with controls (data are presented as the mean ± S.E.; * p

    Article Snippet: Cell proliferation rate was measured with the Click-iT® EdU Alexa Fluor® 488 kit (Invitrogen) according to manufacturer's directions.

    Techniques: Inhibition, Expressing, Fluorescence In Situ Hybridization, Transfection, Labeling, Allele-specific Oligonucleotide, Fluorescence

    ASK does not affect viability of normal cells. a , HFK, HREC, and HnEMs were seeded at 5 × 10 4 cells per well in 12-well plates. On the next day the cells were transfected with ASO-1537S conjugated to Alexa Fluor 488 under the conditions listed in Table 1 . At 24 h post-transfection at least 90% of the cells were fluorescently labeled (fluorescence, left panel ; phase contrast, right panel in each case) ( bars = 20 μm). b , HFK ( colored graph ), HUVEC, HREC, and HnEMs were seeded at 5 × 10 4 cells/well in 12-well plates and transfected the next day with ASO-C or ASO-1537S (see Table 1 ) or left untreated (NT ). Cells treated for 48 h were counted by Tb exclusion. A triplicate analysis showed that viability of these cells was unaffected by ASK. c , HFK transfected as in b for 48 h were harvested, counted, and subjected to RNA extraction followed by RT-PCR amplification. A representative gel shows that ASO-1537S induces a decrease in levels of AS-1 and AS-2. The 18 S rRNA was used as the loading control ( M , 100-bp ladder). d , same as in b , but harvested cells were fixed, stained with PI and analyzed by Flow cytometry. STP was used as a positive cell death control. A triplicate analysis revealed that only STP induced a significant sub-G 1 DNA fraction (60%) in HFKs (*, p

    Journal: The Journal of Biological Chemistry

    Article Title: Down-regulation of the Antisense Mitochondrial Non-coding RNAs (ncRNAs) Is a Unique Vulnerability of Cancer Cells and a Potential Target for Cancer Therapy *

    doi: 10.1074/jbc.M114.558841

    Figure Lengend Snippet: ASK does not affect viability of normal cells. a , HFK, HREC, and HnEMs were seeded at 5 × 10 4 cells per well in 12-well plates. On the next day the cells were transfected with ASO-1537S conjugated to Alexa Fluor 488 under the conditions listed in Table 1 . At 24 h post-transfection at least 90% of the cells were fluorescently labeled (fluorescence, left panel ; phase contrast, right panel in each case) ( bars = 20 μm). b , HFK ( colored graph ), HUVEC, HREC, and HnEMs were seeded at 5 × 10 4 cells/well in 12-well plates and transfected the next day with ASO-C or ASO-1537S (see Table 1 ) or left untreated (NT ). Cells treated for 48 h were counted by Tb exclusion. A triplicate analysis showed that viability of these cells was unaffected by ASK. c , HFK transfected as in b for 48 h were harvested, counted, and subjected to RNA extraction followed by RT-PCR amplification. A representative gel shows that ASO-1537S induces a decrease in levels of AS-1 and AS-2. The 18 S rRNA was used as the loading control ( M , 100-bp ladder). d , same as in b , but harvested cells were fixed, stained with PI and analyzed by Flow cytometry. STP was used as a positive cell death control. A triplicate analysis revealed that only STP induced a significant sub-G 1 DNA fraction (60%) in HFKs (*, p

    Article Snippet: Cell proliferation rate was measured with the Click-iT® EdU Alexa Fluor® 488 kit (Invitrogen) according to manufacturer's directions.

    Techniques: Transfection, Allele-specific Oligonucleotide, Labeling, Fluorescence, RNA Extraction, Reverse Transcription Polymerase Chain Reaction, Amplification, Staining, Flow Cytometry, Cytometry