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  • 99
    Bio-Rad clarity western ecl enhanced chemiluminescence substrate
    Antigenic profiles of the human anti-EV-A71 antibodies. (A) Control cell lysates were loaded into SDS-PAGE gel electrophoresis. Recombinant EV-A71-EGFP cell lysates (structural and non-structural proteins) were probed with anti-GFP-HRP, while recombinant EV-A71 2A cell lysates were stained with Coomassie brilliant blue R-250. EV-A71 virion proteins were immunodetected with EV-A71-specific mAb 3323 (Millipore, USA) and mAb 979 (Millipore, USA), followed by secondary anti-mouse IgG-HRP. The expected band for each individual recombinant protein is indicated by red solid arrows and the protein sizes are shown. (B) Acute infection with no neutralization sera (n = 2) and (C) acute infection with high neutralization sera (n = 12) were used for EV-A71-specific IgM antibody detection. (D) Acute infection with high neutralization sera (n = 12) and (E) convalescent sera (n = 5) were used for EV-A71-specific IgG antibody detection. An estimated 20 μg of proteins was loaded for SDS-PAGE gel electrophoresis. The amount of EV-A71 structural and non-structural protein cell lysates was normalized with anti-GFP-HRP since the presence of inhibitory factors affected accurate quantitation of total proteins. The EV-A71 protein cell lysates and EV-A71 proteins were subjected to SDS-PAGE gel electrophoresis and probed with pooled human sera at a dilution of 1:300. The <t>immunoblot</t> was developed with Clarity Western <t>ECL</t> substrate and detected by chemiluminescence. Protein bands were determined using the Precision Plus Protein WesternC Standard (Bio-Rad, USA). The antigens recognized by EV-A71-infected patient sera are indicated by red solid arrows.
    Clarity Western Ecl Enhanced Chemiluminescence Substrate, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 2460 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 2460 article reviews
    Price from $9.99 to $1999.99
    clarity western ecl enhanced chemiluminescence substrate - by Bioz Stars, 2020-08
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    93
    Bio-Rad clarity western ecl substrate reagent
    Antigenic profiles of the human anti-EV-A71 antibodies. (A) Control cell lysates were loaded into SDS-PAGE gel electrophoresis. Recombinant EV-A71-EGFP cell lysates (structural and non-structural proteins) were probed with anti-GFP-HRP, while recombinant EV-A71 2A cell lysates were stained with Coomassie brilliant blue R-250. EV-A71 virion proteins were immunodetected with EV-A71-specific mAb 3323 (Millipore, USA) and mAb 979 (Millipore, USA), followed by secondary anti-mouse IgG-HRP. The expected band for each individual recombinant protein is indicated by red solid arrows and the protein sizes are shown. (B) Acute infection with no neutralization sera (n = 2) and (C) acute infection with high neutralization sera (n = 12) were used for EV-A71-specific IgM antibody detection. (D) Acute infection with high neutralization sera (n = 12) and (E) convalescent sera (n = 5) were used for EV-A71-specific IgG antibody detection. An estimated 20 μg of proteins was loaded for SDS-PAGE gel electrophoresis. The amount of EV-A71 structural and non-structural protein cell lysates was normalized with anti-GFP-HRP since the presence of inhibitory factors affected accurate quantitation of total proteins. The EV-A71 protein cell lysates and EV-A71 proteins were subjected to SDS-PAGE gel electrophoresis and probed with pooled human sera at a dilution of 1:300. The <t>immunoblot</t> was developed with Clarity Western <t>ECL</t> substrate and detected by chemiluminescence. Protein bands were determined using the Precision Plus Protein WesternC Standard (Bio-Rad, USA). The antigens recognized by EV-A71-infected patient sera are indicated by red solid arrows.
    Clarity Western Ecl Substrate Reagent, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/clarity western ecl substrate reagent/product/Bio-Rad
    Average 93 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    clarity western ecl substrate reagent - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    99
    Bio-Rad clarity max western ecl substrate
    Antigenic profiles of the human anti-EV-A71 antibodies. (A) Control cell lysates were loaded into SDS-PAGE gel electrophoresis. Recombinant EV-A71-EGFP cell lysates (structural and non-structural proteins) were probed with anti-GFP-HRP, while recombinant EV-A71 2A cell lysates were stained with Coomassie brilliant blue R-250. EV-A71 virion proteins were immunodetected with EV-A71-specific mAb 3323 (Millipore, USA) and mAb 979 (Millipore, USA), followed by secondary anti-mouse IgG-HRP. The expected band for each individual recombinant protein is indicated by red solid arrows and the protein sizes are shown. (B) Acute infection with no neutralization sera (n = 2) and (C) acute infection with high neutralization sera (n = 12) were used for EV-A71-specific IgM antibody detection. (D) Acute infection with high neutralization sera (n = 12) and (E) convalescent sera (n = 5) were used for EV-A71-specific IgG antibody detection. An estimated 20 μg of proteins was loaded for SDS-PAGE gel electrophoresis. The amount of EV-A71 structural and non-structural protein cell lysates was normalized with anti-GFP-HRP since the presence of inhibitory factors affected accurate quantitation of total proteins. The EV-A71 protein cell lysates and EV-A71 proteins were subjected to SDS-PAGE gel electrophoresis and probed with pooled human sera at a dilution of 1:300. The <t>immunoblot</t> was developed with Clarity Western <t>ECL</t> substrate and detected by chemiluminescence. Protein bands were determined using the Precision Plus Protein WesternC Standard (Bio-Rad, USA). The antigens recognized by EV-A71-infected patient sera are indicated by red solid arrows.
    Clarity Max Western Ecl Substrate, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 245 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/clarity max western ecl substrate/product/Bio-Rad
    Average 99 stars, based on 245 article reviews
    Price from $9.99 to $1999.99
    clarity max western ecl substrate - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    Image Search Results


    Antigenic profiles of the human anti-EV-A71 antibodies. (A) Control cell lysates were loaded into SDS-PAGE gel electrophoresis. Recombinant EV-A71-EGFP cell lysates (structural and non-structural proteins) were probed with anti-GFP-HRP, while recombinant EV-A71 2A cell lysates were stained with Coomassie brilliant blue R-250. EV-A71 virion proteins were immunodetected with EV-A71-specific mAb 3323 (Millipore, USA) and mAb 979 (Millipore, USA), followed by secondary anti-mouse IgG-HRP. The expected band for each individual recombinant protein is indicated by red solid arrows and the protein sizes are shown. (B) Acute infection with no neutralization sera (n = 2) and (C) acute infection with high neutralization sera (n = 12) were used for EV-A71-specific IgM antibody detection. (D) Acute infection with high neutralization sera (n = 12) and (E) convalescent sera (n = 5) were used for EV-A71-specific IgG antibody detection. An estimated 20 μg of proteins was loaded for SDS-PAGE gel electrophoresis. The amount of EV-A71 structural and non-structural protein cell lysates was normalized with anti-GFP-HRP since the presence of inhibitory factors affected accurate quantitation of total proteins. The EV-A71 protein cell lysates and EV-A71 proteins were subjected to SDS-PAGE gel electrophoresis and probed with pooled human sera at a dilution of 1:300. The immunoblot was developed with Clarity Western ECL substrate and detected by chemiluminescence. Protein bands were determined using the Precision Plus Protein WesternC Standard (Bio-Rad, USA). The antigens recognized by EV-A71-infected patient sera are indicated by red solid arrows.

    Journal: PLoS ONE

    Article Title: Immunodominant IgM and IgG Epitopes Recognized by Antibodies Induced in Enterovirus A71-Associated Hand, Foot and Mouth Disease Patients

    doi: 10.1371/journal.pone.0165659

    Figure Lengend Snippet: Antigenic profiles of the human anti-EV-A71 antibodies. (A) Control cell lysates were loaded into SDS-PAGE gel electrophoresis. Recombinant EV-A71-EGFP cell lysates (structural and non-structural proteins) were probed with anti-GFP-HRP, while recombinant EV-A71 2A cell lysates were stained with Coomassie brilliant blue R-250. EV-A71 virion proteins were immunodetected with EV-A71-specific mAb 3323 (Millipore, USA) and mAb 979 (Millipore, USA), followed by secondary anti-mouse IgG-HRP. The expected band for each individual recombinant protein is indicated by red solid arrows and the protein sizes are shown. (B) Acute infection with no neutralization sera (n = 2) and (C) acute infection with high neutralization sera (n = 12) were used for EV-A71-specific IgM antibody detection. (D) Acute infection with high neutralization sera (n = 12) and (E) convalescent sera (n = 5) were used for EV-A71-specific IgG antibody detection. An estimated 20 μg of proteins was loaded for SDS-PAGE gel electrophoresis. The amount of EV-A71 structural and non-structural protein cell lysates was normalized with anti-GFP-HRP since the presence of inhibitory factors affected accurate quantitation of total proteins. The EV-A71 protein cell lysates and EV-A71 proteins were subjected to SDS-PAGE gel electrophoresis and probed with pooled human sera at a dilution of 1:300. The immunoblot was developed with Clarity Western ECL substrate and detected by chemiluminescence. Protein bands were determined using the Precision Plus Protein WesternC Standard (Bio-Rad, USA). The antigens recognized by EV-A71-infected patient sera are indicated by red solid arrows.

    Article Snippet: The immunoblot was developed with Clarity Western ECL Substrate (Bio-Rad, USA) and detected by chemiluminescence.

    Techniques: SDS Page, Nucleic Acid Electrophoresis, Recombinant, Staining, Infection, Neutralization, Quantitation Assay, Western Blot

    Purification of recombinant 6xHis-tagged hTLK1B from E. coli . (A) Schematic overview of the purification process using Ni-NTA IMAC and size exclusion chromatography (SEC). (B) SDS-PAGE (12% Tris-Glycine gel) analysis of recombinant hTLK1B through different purification steps. M, molecular weight marker in kDa; lane 1, uninduced soluble fraction after cell homogenization (control); lane 2, IPTG induced soluble fraction (total cell lysate); lane 3, unbound protein fraction; lane 4-6, after-binding column wash fractions; lane 8, eluted hTLK1B after 200 mM imidazole wash. Arrow indicates the position of recombinant 6xHis-tagged hTLK1B protein (64.9 kDa). (C) Chromatogram of HiLoad 16/60 Superdex 75 size-exclusion column after Ni-NTA IMAC step. (D) Immunoblot showing the purified recombinant 6xHis-tagged hTLK1B protein after SEC. His-Tag (D3I10) XP ® Rabbit Monoclonal Antibody (Cat. 12698) was used as a primary antibody. The protein was detected by using Anti-Rabbit IgG Horseradish Peroxidase-linked Secondary Antibody (Cat. 7074) and ECL as a luminescent substrate. The blot image has been cropped for clarity and conciseness. The full-length image of the blots (with multiple exposures) have been included in the Supplementary Figure, S 5 .

    Journal: Scientific Reports

    Article Title: High yield bacterial expression, purification and characterisation of bioactive Human Tousled-like Kinase 1B involved in cancer

    doi: 10.1038/s41598-018-22744-5

    Figure Lengend Snippet: Purification of recombinant 6xHis-tagged hTLK1B from E. coli . (A) Schematic overview of the purification process using Ni-NTA IMAC and size exclusion chromatography (SEC). (B) SDS-PAGE (12% Tris-Glycine gel) analysis of recombinant hTLK1B through different purification steps. M, molecular weight marker in kDa; lane 1, uninduced soluble fraction after cell homogenization (control); lane 2, IPTG induced soluble fraction (total cell lysate); lane 3, unbound protein fraction; lane 4-6, after-binding column wash fractions; lane 8, eluted hTLK1B after 200 mM imidazole wash. Arrow indicates the position of recombinant 6xHis-tagged hTLK1B protein (64.9 kDa). (C) Chromatogram of HiLoad 16/60 Superdex 75 size-exclusion column after Ni-NTA IMAC step. (D) Immunoblot showing the purified recombinant 6xHis-tagged hTLK1B protein after SEC. His-Tag (D3I10) XP ® Rabbit Monoclonal Antibody (Cat. 12698) was used as a primary antibody. The protein was detected by using Anti-Rabbit IgG Horseradish Peroxidase-linked Secondary Antibody (Cat. 7074) and ECL as a luminescent substrate. The blot image has been cropped for clarity and conciseness. The full-length image of the blots (with multiple exposures) have been included in the Supplementary Figure, S 5 .

    Article Snippet: Nuvia™ Immobilized Metal Affinity Chromatography (IMAC) Resin charged with Ni2+ , Econo-Column® Chromatography Columns and Clarity™ ECL Western Blotting Substrate were purchased from Bio-Rad Laboratories (Hercules, CA, USA).

    Techniques: Purification, Recombinant, Size-exclusion Chromatography, SDS Page, Molecular Weight, Marker, Homogenization, Binding Assay