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Promega
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Promega
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Promega
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ICN Biomedicals
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Promega
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GenScript corporation
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ACGT Inc
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Promega
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GenScript corporation
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Image Search Results
Journal: PLoS ONE
Article Title: Engineered Production of Short Chain Fatty Acid in Escherichia coli Using Fatty Acid Synthesis Pathway
doi: 10.1371/journal.pone.0160035
Figure Lengend Snippet: Fatty acid synthesis and degradation cycles have been depicted with blue and orange arrows, respectively. Regulatory proteins have been shown in dotted box in green and red color denoting activation and repression, respectively. Genes that have been overexpressed in this study have been denoted with green upward arrow, while genes that have been deleted are shown along with red cross. Abbreviations: TesAT—thioesterase of Anaerococcus tetradius ; TesBT–thioesterase of Bacteroides thetaiotaomicron ; TesBF—thioesterase of Bryantella formatexigens ; Acc*—acetyl-CoA carboxylase of Corynebacterium glutamicum ; FabD–malonyl CoA-ACP transacylase; FabH - β- ketoacyl-ACP synthase III; FabG - β-ketoacyl-ACP reductase; FabA and FabZ - β-hydroxyacyl-ACP dehydratase; FabI—enoyl-ACP reductase; FabB - β-ketoacyl-ACP synthase I; FadD—fatty acyl-CoA synthetase; FadE—acyl CoA dehydrogenase; FabR–transcriptional repressor of FASII; FadR–transcriptional enhancer of FASII and repressor of β-oxidation.
Article Snippet:
Techniques: Activation Assay
Journal: PLoS ONE
Article Title: Engineered Production of Short Chain Fatty Acid in Escherichia coli Using Fatty Acid Synthesis Pathway
doi: 10.1371/journal.pone.0160035
Figure Lengend Snippet: E . coli MG1655 was co-transformed with plasmid containing gene for thioesterase TesBT along with the plasmid containing gene(s) having positive on host fatty acid biosynthesis and analyzed for production of various chain length free fatty acids. Gene function: fabZ - β-hydroxyacyl-ACP dehydratase; fadR –transcriptional enhancer of FASII and repressor of β-oxidation; acc –acetyl-CoA carboxylase of Corynebacterium glutamicum .
Article Snippet:
Techniques: Transformation Assay, Plasmid Preparation