chromatographic conditions hptlc Search Results


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  • 99
    Millipore thin layer chromatography tlc
    Thin Layer Chromatography Tlc, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 304 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Camag automatic tlc sampler 4
    Automatic Tlc Sampler 4, supplied by Camag, used in various techniques. Bioz Stars score: 89/100, based on 234 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Camag tlc scanner 3
    Tlc Scanner 3, supplied by Camag, used in various techniques. Bioz Stars score: 92/100, based on 906 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Camag camag linomat v
    Camag Linomat V, supplied by Camag, used in various techniques. Bioz Stars score: 89/100, based on 147 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore oleanolic acid
    Fluorescence microscopy of A549 cell line treated with MPE, MPPs and <t>oleanolic</t> acid. (Control–untreated, A –vanillic aldehyde, B –caffeic acid, C –oleanolic acid, D –chlorogenic acid, E –gallic acid, F –procyanidin B 2 , G –5-fluorouracil, MPE–mango peel extract).
    Oleanolic Acid, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 187 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Merck KGaA silica gel 60f254
    Fluorescence microscopy of A549 cell line treated with MPE, MPPs and <t>oleanolic</t> acid. (Control–untreated, A –vanillic aldehyde, B –caffeic acid, C –oleanolic acid, D –chlorogenic acid, E –gallic acid, F –procyanidin B 2 , G –5-fluorouracil, MPE–mango peel extract).
    Silica Gel 60f254, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 91/100, based on 260 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Camag hptlc system
    Fluorescence microscopy of A549 cell line treated with MPE, MPPs and <t>oleanolic</t> acid. (Control–untreated, A –vanillic aldehyde, B –caffeic acid, C –oleanolic acid, D –chlorogenic acid, E –gallic acid, F –procyanidin B 2 , G –5-fluorouracil, MPE–mango peel extract).
    Hptlc System, supplied by Camag, used in various techniques. Bioz Stars score: 90/100, based on 475 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Camag camag tlc scanner 3
    Fluorescence microscopy of A549 cell line treated with MPE, MPPs and <t>oleanolic</t> acid. (Control–untreated, A –vanillic aldehyde, B –caffeic acid, C –oleanolic acid, D –chlorogenic acid, E –gallic acid, F –procyanidin B 2 , G –5-fluorouracil, MPE–mango peel extract).
    Camag Tlc Scanner 3, supplied by Camag, used in various techniques. Bioz Stars score: 90/100, based on 231 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Fuji Pharma chromatorex ods dm1020t
    Fluorescence microscopy of A549 cell line treated with MPE, MPPs and <t>oleanolic</t> acid. (Control–untreated, A –vanillic aldehyde, B –caffeic acid, C –oleanolic acid, D –chlorogenic acid, E –gallic acid, F –procyanidin B 2 , G –5-fluorouracil, MPE–mango peel extract).
    Chromatorex Ods Dm1020t, supplied by Fuji Pharma, used in various techniques. Bioz Stars score: 88/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck & Co silica gel rp 18 wf254s
    Fluorescence microscopy of A549 cell line treated with MPE, MPPs and <t>oleanolic</t> acid. (Control–untreated, A –vanillic aldehyde, B –caffeic acid, C –oleanolic acid, D –chlorogenic acid, E –gallic acid, F –procyanidin B 2 , G –5-fluorouracil, MPE–mango peel extract).
    Silica Gel Rp 18 Wf254s, supplied by Merck & Co, used in various techniques. Bioz Stars score: 88/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    JEOL jnm ecs400
    Fluorescence microscopy of A549 cell line treated with MPE, MPPs and <t>oleanolic</t> acid. (Control–untreated, A –vanillic aldehyde, B –caffeic acid, C –oleanolic acid, D –chlorogenic acid, E –gallic acid, F –procyanidin B 2 , G –5-fluorouracil, MPE–mango peel extract).
    Jnm Ecs400, supplied by JEOL, used in various techniques. Bioz Stars score: 88/100, based on 116 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    JEOL h nmr spectra
    Fluorescence microscopy of A549 cell line treated with MPE, MPPs and <t>oleanolic</t> acid. (Control–untreated, A –vanillic aldehyde, B –caffeic acid, C –oleanolic acid, D –chlorogenic acid, E –gallic acid, F –procyanidin B 2 , G –5-fluorouracil, MPE–mango peel extract).
    H Nmr Spectra, supplied by JEOL, used in various techniques. Bioz Stars score: 92/100, based on 616 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    JEOL c nmr spectra
    Fluorescence microscopy of A549 cell line treated with MPE, MPPs and <t>oleanolic</t> acid. (Control–untreated, A –vanillic aldehyde, B –caffeic acid, C –oleanolic acid, D –chlorogenic acid, E –gallic acid, F –procyanidin B 2 , G –5-fluorouracil, MPE–mango peel extract).
    C Nmr Spectra, supplied by JEOL, used in various techniques. Bioz Stars score: 93/100, based on 1407 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Camag camag microlitre syringe
    Fluorescence microscopy of A549 cell line treated with MPE, MPPs and <t>oleanolic</t> acid. (Control–untreated, A –vanillic aldehyde, B –caffeic acid, C –oleanolic acid, D –chlorogenic acid, E –gallic acid, F –procyanidin B 2 , G –5-fluorouracil, MPE–mango peel extract).
    Camag Microlitre Syringe, supplied by Camag, used in various techniques. Bioz Stars score: 88/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Nacalai cosmosil 5c18 ms ii
    Fluorescence microscopy of A549 cell line treated with MPE, MPPs and <t>oleanolic</t> acid. (Control–untreated, A –vanillic aldehyde, B –caffeic acid, C –oleanolic acid, D –chlorogenic acid, E –gallic acid, F –procyanidin B 2 , G –5-fluorouracil, MPE–mango peel extract).
    Cosmosil 5c18 Ms Ii, supplied by Nacalai, used in various techniques. Bioz Stars score: 88/100, based on 72 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Nacalai hplc column
    Fluorescence microscopy of A549 cell line treated with MPE, MPPs and <t>oleanolic</t> acid. (Control–untreated, A –vanillic aldehyde, B –caffeic acid, C –oleanolic acid, D –chlorogenic acid, E –gallic acid, F –procyanidin B 2 , G –5-fluorouracil, MPE–mango peel extract).
    Hplc Column, supplied by Nacalai, used in various techniques. Bioz Stars score: 92/100, based on 58 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Shimadzu Corporation hplc detector
    Fluorescence microscopy of A549 cell line treated with MPE, MPPs and <t>oleanolic</t> acid. (Control–untreated, A –vanillic aldehyde, B –caffeic acid, C –oleanolic acid, D –chlorogenic acid, E –gallic acid, F –procyanidin B 2 , G –5-fluorouracil, MPE–mango peel extract).
    Hplc Detector, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 88/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore omeprazole
    Effects of <t>omeprazole</t> on HCC cell line viability and on the tumor microenvironment of HCC tissue explants. (A) The protein expression of a1, C1, β-actin and α-tubulin was assessed by western blotting in cell lysates of the C3A, PLC/PRF/5 and SNU-449 cell lines. (B) The HCC cell lines were treated with different doses of omeprazole, and cell viability was evaluated using the MTT assay. The data on the y-axes report the percentage of viable cells of the untreated control and represent the mean of six replicate reactions from 3 independent experiments. The calculated IC 50 values for omeprazole are 100.9, 39.4, and 128.4 μg/ml for C3A, PLC/PRF/5 and SNU-449 respectively. (C) Expression levels of the indicated genes in HCC tissue explants ( MYC, VIM, CDH1, CCL22, IFNG N = 12; TNF , N = 8) cultured ex vivo in the presence of 100 μg/ml omeprazole or the vehicle of the drug for 24 h. The gene expression data are reported as 2 −ΔCt values. The p-values are calculated by the paired t test comparing cells treated with omeprazole or the vehicle.
    Omeprazole, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 468 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck & Co silica gel rp 18 f254s
    Effects of <t>omeprazole</t> on HCC cell line viability and on the tumor microenvironment of HCC tissue explants. (A) The protein expression of a1, C1, β-actin and α-tubulin was assessed by western blotting in cell lysates of the C3A, PLC/PRF/5 and SNU-449 cell lines. (B) The HCC cell lines were treated with different doses of omeprazole, and cell viability was evaluated using the MTT assay. The data on the y-axes report the percentage of viable cells of the untreated control and represent the mean of six replicate reactions from 3 independent experiments. The calculated IC 50 values for omeprazole are 100.9, 39.4, and 128.4 μg/ml for C3A, PLC/PRF/5 and SNU-449 respectively. (C) Expression levels of the indicated genes in HCC tissue explants ( MYC, VIM, CDH1, CCL22, IFNG N = 12; TNF , N = 8) cultured ex vivo in the presence of 100 μg/ml omeprazole or the vehicle of the drug for 24 h. The gene expression data are reported as 2 −ΔCt values. The p-values are calculated by the paired t test comparing cells treated with omeprazole or the vehicle.
    Silica Gel Rp 18 F254s, supplied by Merck & Co, used in various techniques. Bioz Stars score: 88/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck KGaA normal phase tlc
    Effects of <t>omeprazole</t> on HCC cell line viability and on the tumor microenvironment of HCC tissue explants. (A) The protein expression of a1, C1, β-actin and α-tubulin was assessed by western blotting in cell lysates of the C3A, PLC/PRF/5 and SNU-449 cell lines. (B) The HCC cell lines were treated with different doses of omeprazole, and cell viability was evaluated using the MTT assay. The data on the y-axes report the percentage of viable cells of the untreated control and represent the mean of six replicate reactions from 3 independent experiments. The calculated IC 50 values for omeprazole are 100.9, 39.4, and 128.4 μg/ml for C3A, PLC/PRF/5 and SNU-449 respectively. (C) Expression levels of the indicated genes in HCC tissue explants ( MYC, VIM, CDH1, CCL22, IFNG N = 12; TNF , N = 8) cultured ex vivo in the presence of 100 μg/ml omeprazole or the vehicle of the drug for 24 h. The gene expression data are reported as 2 −ΔCt values. The p-values are calculated by the paired t test comparing cells treated with omeprazole or the vehicle.
    Normal Phase Tlc, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 88/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck KGaA pre coated tlc plates
    Effects of <t>omeprazole</t> on HCC cell line viability and on the tumor microenvironment of HCC tissue explants. (A) The protein expression of a1, C1, β-actin and α-tubulin was assessed by western blotting in cell lysates of the C3A, PLC/PRF/5 and SNU-449 cell lines. (B) The HCC cell lines were treated with different doses of omeprazole, and cell viability was evaluated using the MTT assay. The data on the y-axes report the percentage of viable cells of the untreated control and represent the mean of six replicate reactions from 3 independent experiments. The calculated IC 50 values for omeprazole are 100.9, 39.4, and 128.4 μg/ml for C3A, PLC/PRF/5 and SNU-449 respectively. (C) Expression levels of the indicated genes in HCC tissue explants ( MYC, VIM, CDH1, CCL22, IFNG N = 12; TNF , N = 8) cultured ex vivo in the presence of 100 μg/ml omeprazole or the vehicle of the drug for 24 h. The gene expression data are reported as 2 −ΔCt values. The p-values are calculated by the paired t test comparing cells treated with omeprazole or the vehicle.
    Pre Coated Tlc Plates, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 89/100, based on 203 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck & Co reversed phase tlc
    Effects of <t>omeprazole</t> on HCC cell line viability and on the tumor microenvironment of HCC tissue explants. (A) The protein expression of a1, C1, β-actin and α-tubulin was assessed by western blotting in cell lysates of the C3A, PLC/PRF/5 and SNU-449 cell lines. (B) The HCC cell lines were treated with different doses of omeprazole, and cell viability was evaluated using the MTT assay. The data on the y-axes report the percentage of viable cells of the untreated control and represent the mean of six replicate reactions from 3 independent experiments. The calculated IC 50 values for omeprazole are 100.9, 39.4, and 128.4 μg/ml for C3A, PLC/PRF/5 and SNU-449 respectively. (C) Expression levels of the indicated genes in HCC tissue explants ( MYC, VIM, CDH1, CCL22, IFNG N = 12; TNF , N = 8) cultured ex vivo in the presence of 100 μg/ml omeprazole or the vehicle of the drug for 24 h. The gene expression data are reported as 2 −ΔCt values. The p-values are calculated by the paired t test comparing cells treated with omeprazole or the vehicle.
    Reversed Phase Tlc, supplied by Merck & Co, used in various techniques. Bioz Stars score: 88/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Shimadzu Corporation uv spectra
    Effects of <t>omeprazole</t> on HCC cell line viability and on the tumor microenvironment of HCC tissue explants. (A) The protein expression of a1, C1, β-actin and α-tubulin was assessed by western blotting in cell lysates of the C3A, PLC/PRF/5 and SNU-449 cell lines. (B) The HCC cell lines were treated with different doses of omeprazole, and cell viability was evaluated using the MTT assay. The data on the y-axes report the percentage of viable cells of the untreated control and represent the mean of six replicate reactions from 3 independent experiments. The calculated IC 50 values for omeprazole are 100.9, 39.4, and 128.4 μg/ml for C3A, PLC/PRF/5 and SNU-449 respectively. (C) Expression levels of the indicated genes in HCC tissue explants ( MYC, VIM, CDH1, CCL22, IFNG N = 12; TNF , N = 8) cultured ex vivo in the presence of 100 μg/ml omeprazole or the vehicle of the drug for 24 h. The gene expression data are reported as 2 −ΔCt values. The p-values are calculated by the paired t test comparing cells treated with omeprazole or the vehicle.
    Uv Spectra, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 94/100, based on 2575 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Fluorescence microscopy of A549 cell line treated with MPE, MPPs and oleanolic acid. (Control–untreated, A –vanillic aldehyde, B –caffeic acid, C –oleanolic acid, D –chlorogenic acid, E –gallic acid, F –procyanidin B 2 , G –5-fluorouracil, MPE–mango peel extract).

    Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

    Article Title: In Vitro Antioxidant Activities of Phenols and Oleanolic Acid from Mango Peel and Their Cytotoxic Effect on A549 Cell Line

    doi: 10.3390/molecules23061395

    Figure Lengend Snippet: Fluorescence microscopy of A549 cell line treated with MPE, MPPs and oleanolic acid. (Control–untreated, A –vanillic aldehyde, B –caffeic acid, C –oleanolic acid, D –chlorogenic acid, E –gallic acid, F –procyanidin B 2 , G –5-fluorouracil, MPE–mango peel extract).

    Article Snippet: HPLC Analysis of MPPs and Oleanolic Acid Five phenolic compounds, vanillic aldehyde, caffeic acid, chlorogenic acid, gallicacid, procyanidin B2 , and oleanolic acid , and 5-fluorouracil were purchased from Sigma-Aldrich Chemical (St. Louis, MO, USA) and respectively dissolved in methanol to a final concentration of 100 μM.

    Techniques: Fluorescence, Microscopy

    Chemical structures of detected compounds in MPE. ( A –vanillic aldehyde; B –caffeic acid; C –oleanolic acid; D –chlorogenic acid; E –gallic acid; F –procyanidin B 2 ).

    Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

    Article Title: In Vitro Antioxidant Activities of Phenols and Oleanolic Acid from Mango Peel and Their Cytotoxic Effect on A549 Cell Line

    doi: 10.3390/molecules23061395

    Figure Lengend Snippet: Chemical structures of detected compounds in MPE. ( A –vanillic aldehyde; B –caffeic acid; C –oleanolic acid; D –chlorogenic acid; E –gallic acid; F –procyanidin B 2 ).

    Article Snippet: HPLC Analysis of MPPs and Oleanolic Acid Five phenolic compounds, vanillic aldehyde, caffeic acid, chlorogenic acid, gallicacid, procyanidin B2 , and oleanolic acid , and 5-fluorouracil were purchased from Sigma-Aldrich Chemical (St. Louis, MO, USA) and respectively dissolved in methanol to a final concentration of 100 μM.

    Techniques:

    HPLC chromatography profile of mango peel extract (MPE). ( A –vanillic aldehyde; B –caffeic acid; C –oleanolic acid; D –chlorogenic acid; E –gallic acid; F –procyanidin B 2 ).

    Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

    Article Title: In Vitro Antioxidant Activities of Phenols and Oleanolic Acid from Mango Peel and Their Cytotoxic Effect on A549 Cell Line

    doi: 10.3390/molecules23061395

    Figure Lengend Snippet: HPLC chromatography profile of mango peel extract (MPE). ( A –vanillic aldehyde; B –caffeic acid; C –oleanolic acid; D –chlorogenic acid; E –gallic acid; F –procyanidin B 2 ).

    Article Snippet: HPLC Analysis of MPPs and Oleanolic Acid Five phenolic compounds, vanillic aldehyde, caffeic acid, chlorogenic acid, gallicacid, procyanidin B2 , and oleanolic acid , and 5-fluorouracil were purchased from Sigma-Aldrich Chemical (St. Louis, MO, USA) and respectively dissolved in methanol to a final concentration of 100 μM.

    Techniques: High Performance Liquid Chromatography, Chromatography

    Effects of omeprazole on HCC cell line viability and on the tumor microenvironment of HCC tissue explants. (A) The protein expression of a1, C1, β-actin and α-tubulin was assessed by western blotting in cell lysates of the C3A, PLC/PRF/5 and SNU-449 cell lines. (B) The HCC cell lines were treated with different doses of omeprazole, and cell viability was evaluated using the MTT assay. The data on the y-axes report the percentage of viable cells of the untreated control and represent the mean of six replicate reactions from 3 independent experiments. The calculated IC 50 values for omeprazole are 100.9, 39.4, and 128.4 μg/ml for C3A, PLC/PRF/5 and SNU-449 respectively. (C) Expression levels of the indicated genes in HCC tissue explants ( MYC, VIM, CDH1, CCL22, IFNG N = 12; TNF , N = 8) cultured ex vivo in the presence of 100 μg/ml omeprazole or the vehicle of the drug for 24 h. The gene expression data are reported as 2 −ΔCt values. The p-values are calculated by the paired t test comparing cells treated with omeprazole or the vehicle.

    Journal: Oncoimmunology

    Article Title: pH regulators to target the tumor immune microenvironment in human hepatocellular carcinoma

    doi: 10.1080/2162402X.2018.1445452

    Figure Lengend Snippet: Effects of omeprazole on HCC cell line viability and on the tumor microenvironment of HCC tissue explants. (A) The protein expression of a1, C1, β-actin and α-tubulin was assessed by western blotting in cell lysates of the C3A, PLC/PRF/5 and SNU-449 cell lines. (B) The HCC cell lines were treated with different doses of omeprazole, and cell viability was evaluated using the MTT assay. The data on the y-axes report the percentage of viable cells of the untreated control and represent the mean of six replicate reactions from 3 independent experiments. The calculated IC 50 values for omeprazole are 100.9, 39.4, and 128.4 μg/ml for C3A, PLC/PRF/5 and SNU-449 respectively. (C) Expression levels of the indicated genes in HCC tissue explants ( MYC, VIM, CDH1, CCL22, IFNG N = 12; TNF , N = 8) cultured ex vivo in the presence of 100 μg/ml omeprazole or the vehicle of the drug for 24 h. The gene expression data are reported as 2 −ΔCt values. The p-values are calculated by the paired t test comparing cells treated with omeprazole or the vehicle.

    Article Snippet: Fresh HCC tissues (n = 5) were cut into 3 mm3 pieces using a biopsy puncher under sterile conditions and were cultured for 24 h in a 48-well plate (Corning) in the presence of 300 μl RPMI 1640 with 1% FCS (Lonza) and 100 µg/ml omeprazole (Sigma-Aldrich) or drug vehicle (dimethylsulfoxide, DMSO, Sigma-Aldrich).

    Techniques: Expressing, Western Blot, Planar Chromatography, MTT Assay, Cell Culture, Ex Vivo

    Modulation of the cytokine profile in HCC immune-infiltrating cells by omeprazole treatment. (A-D) Multiparametric flow cytometry of live cells in freshly dissociated HCC tumors treated with the drug vehicle (red line) or with omeprazole (100 μg/ml, blue line) for 24 h. IFNγ, TNF and CCL22 production was evaluated by intracellular staining (A) in CD163 + myeloid cells gated inside live CD45 + CD3 − cells and (B) in CD3 + T cells gated inside live CD45 + cells. FMO control for each cytokine (gray line) is reported in the histogram plot. The percentages of IFNγ-, TNF- and CCL22-positive cells in the CD163 + and in the CD3 + cells are shown in C and D respectively. The p-values are calculated by the paired t test comparing cells treated with omeprazole or the vehicle.

    Journal: Oncoimmunology

    Article Title: pH regulators to target the tumor immune microenvironment in human hepatocellular carcinoma

    doi: 10.1080/2162402X.2018.1445452

    Figure Lengend Snippet: Modulation of the cytokine profile in HCC immune-infiltrating cells by omeprazole treatment. (A-D) Multiparametric flow cytometry of live cells in freshly dissociated HCC tumors treated with the drug vehicle (red line) or with omeprazole (100 μg/ml, blue line) for 24 h. IFNγ, TNF and CCL22 production was evaluated by intracellular staining (A) in CD163 + myeloid cells gated inside live CD45 + CD3 − cells and (B) in CD3 + T cells gated inside live CD45 + cells. FMO control for each cytokine (gray line) is reported in the histogram plot. The percentages of IFNγ-, TNF- and CCL22-positive cells in the CD163 + and in the CD3 + cells are shown in C and D respectively. The p-values are calculated by the paired t test comparing cells treated with omeprazole or the vehicle.

    Article Snippet: Fresh HCC tissues (n = 5) were cut into 3 mm3 pieces using a biopsy puncher under sterile conditions and were cultured for 24 h in a 48-well plate (Corning) in the presence of 300 μl RPMI 1640 with 1% FCS (Lonza) and 100 µg/ml omeprazole (Sigma-Aldrich) or drug vehicle (dimethylsulfoxide, DMSO, Sigma-Aldrich).

    Techniques: Flow Cytometry, Cytometry, Staining