chromatographic condition chromatography Search Results


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  • 91
    Waters Corporation chromatographic condition chromatography
    Chromatographic Condition Chromatography, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Shimadzu Corporation chromatographic conditions chromatography
    Chromatographic Conditions Chromatography, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies chromatographic conditions chromatography
    Chromatographic Conditions Chromatography, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Waters Corporation liquid chromatographic conditions chromatography
    Liquid Chromatographic Conditions Chromatography, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 86/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Waters Corporation chromatography conditions chromatographic separation
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    Waters Corporation conditions chromatography
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    Agilent technologies liquid chromatographic conditions liquid chromatography
    Liquid Chromatographic Conditions Liquid Chromatography, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies chromatographic conditions uhplc
    The identification of the active binding fraction of RP by CMC. ( a ) The experimental flow of CMC. PC-12 cells were incubated with RP (TEE) for 1 to 6 h. After incubation, chemical components without binding affinity to the cell membrane were washed away by PBS, while those components that bind on cell membrane were retained for analysis. The cells were then disrupted by citric acid buffer with ultrasound sonication. The lysate solution was then centrifuged, dried and re-dissolved in methanol. Cell lysate without RP incubation was collected as control. Finally, all the collected samples were analyzed using <t>UHPLC-TOF-MS.</t> ( b ) The Total Ion Chromatogram (TIC) of the 5 different batches of RP (TEE). ( c ) The TIC of the CMC samples. S1: The final PBS wash solution; S2: PC-12 lysate solution without RP (TEE) treatments; S3: RP (TEE) solution diluted with PBS; S4: PC-12 cell lysate solution collected after RP (TEE) treatments. The cluster of peaks (C5) indicated the chemical components that bind on the cell membrane of PC-12 cells. ( d ) The TIC of the CMC samples collected from 5 different batches of RP (TEE) treatments. A: PC-12 lysate solution without RP (TEE) treatments; B: The final PBS wash solution; C, E, G, I, K: 5 different batches of RP (TEE) solution diluted with PBS; D, F, H, J, L: PC-12 cell lysate solution collected after treatments of 5 different batches of RP (TEE). ( e ) The TIC of PC-12 cell lysate collected after 1 to 6 h of RP (TEE) treatments (500 μg/mL). ( f ) The TIC of PC-12 cell lysate collected after RP (TEE) treatments (125, 250, 500 or 750 μg/mL) for 4 h. All the samples were analyzed by UHPLC-TOF-MS on an <t>Agilent</t> Zorbax Eclipse Plus C-18 50 mm × 2.1 mm column (particle size: 1.8 μm) at a flow rate of 0.35 mL/min. The data was acquired in the scan mode from m/z 100 to 3200 Da with 2.0 spectra/s.
    Chromatographic Conditions Uhplc, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Waters Corporation chromatographic conditions acquity ultra performance liquid chromatography
    The identification of the active binding fraction of RP by CMC. ( a ) The experimental flow of CMC. PC-12 cells were incubated with RP (TEE) for 1 to 6 h. After incubation, chemical components without binding affinity to the cell membrane were washed away by PBS, while those components that bind on cell membrane were retained for analysis. The cells were then disrupted by citric acid buffer with ultrasound sonication. The lysate solution was then centrifuged, dried and re-dissolved in methanol. Cell lysate without RP incubation was collected as control. Finally, all the collected samples were analyzed using <t>UHPLC-TOF-MS.</t> ( b ) The Total Ion Chromatogram (TIC) of the 5 different batches of RP (TEE). ( c ) The TIC of the CMC samples. S1: The final PBS wash solution; S2: PC-12 lysate solution without RP (TEE) treatments; S3: RP (TEE) solution diluted with PBS; S4: PC-12 cell lysate solution collected after RP (TEE) treatments. The cluster of peaks (C5) indicated the chemical components that bind on the cell membrane of PC-12 cells. ( d ) The TIC of the CMC samples collected from 5 different batches of RP (TEE) treatments. A: PC-12 lysate solution without RP (TEE) treatments; B: The final PBS wash solution; C, E, G, I, K: 5 different batches of RP (TEE) solution diluted with PBS; D, F, H, J, L: PC-12 cell lysate solution collected after treatments of 5 different batches of RP (TEE). ( e ) The TIC of PC-12 cell lysate collected after 1 to 6 h of RP (TEE) treatments (500 μg/mL). ( f ) The TIC of PC-12 cell lysate collected after RP (TEE) treatments (125, 250, 500 or 750 μg/mL) for 4 h. All the samples were analyzed by UHPLC-TOF-MS on an <t>Agilent</t> Zorbax Eclipse Plus C-18 50 mm × 2.1 mm column (particle size: 1.8 μm) at a flow rate of 0.35 mL/min. The data was acquired in the scan mode from m/z 100 to 3200 Da with 2.0 spectra/s.
    Chromatographic Conditions Acquity Ultra Performance Liquid Chromatography, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Shimadzu Corporation analytical conditions chromatography analysis
    The identification of the active binding fraction of RP by CMC. ( a ) The experimental flow of CMC. PC-12 cells were incubated with RP (TEE) for 1 to 6 h. After incubation, chemical components without binding affinity to the cell membrane were washed away by PBS, while those components that bind on cell membrane were retained for analysis. The cells were then disrupted by citric acid buffer with ultrasound sonication. The lysate solution was then centrifuged, dried and re-dissolved in methanol. Cell lysate without RP incubation was collected as control. Finally, all the collected samples were analyzed using <t>UHPLC-TOF-MS.</t> ( b ) The Total Ion Chromatogram (TIC) of the 5 different batches of RP (TEE). ( c ) The TIC of the CMC samples. S1: The final PBS wash solution; S2: PC-12 lysate solution without RP (TEE) treatments; S3: RP (TEE) solution diluted with PBS; S4: PC-12 cell lysate solution collected after RP (TEE) treatments. The cluster of peaks (C5) indicated the chemical components that bind on the cell membrane of PC-12 cells. ( d ) The TIC of the CMC samples collected from 5 different batches of RP (TEE) treatments. A: PC-12 lysate solution without RP (TEE) treatments; B: The final PBS wash solution; C, E, G, I, K: 5 different batches of RP (TEE) solution diluted with PBS; D, F, H, J, L: PC-12 cell lysate solution collected after treatments of 5 different batches of RP (TEE). ( e ) The TIC of PC-12 cell lysate collected after 1 to 6 h of RP (TEE) treatments (500 μg/mL). ( f ) The TIC of PC-12 cell lysate collected after RP (TEE) treatments (125, 250, 500 or 750 μg/mL) for 4 h. All the samples were analyzed by UHPLC-TOF-MS on an <t>Agilent</t> Zorbax Eclipse Plus C-18 50 mm × 2.1 mm column (particle size: 1.8 μm) at a flow rate of 0.35 mL/min. The data was acquired in the scan mode from m/z 100 to 3200 Da with 2.0 spectra/s.
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    Merck KGaA chromatographic conditions high performance liquid chromatography grade acetonitrile
    The identification of the active binding fraction of RP by CMC. ( a ) The experimental flow of CMC. PC-12 cells were incubated with RP (TEE) for 1 to 6 h. After incubation, chemical components without binding affinity to the cell membrane were washed away by PBS, while those components that bind on cell membrane were retained for analysis. The cells were then disrupted by citric acid buffer with ultrasound sonication. The lysate solution was then centrifuged, dried and re-dissolved in methanol. Cell lysate without RP incubation was collected as control. Finally, all the collected samples were analyzed using <t>UHPLC-TOF-MS.</t> ( b ) The Total Ion Chromatogram (TIC) of the 5 different batches of RP (TEE). ( c ) The TIC of the CMC samples. S1: The final PBS wash solution; S2: PC-12 lysate solution without RP (TEE) treatments; S3: RP (TEE) solution diluted with PBS; S4: PC-12 cell lysate solution collected after RP (TEE) treatments. The cluster of peaks (C5) indicated the chemical components that bind on the cell membrane of PC-12 cells. ( d ) The TIC of the CMC samples collected from 5 different batches of RP (TEE) treatments. A: PC-12 lysate solution without RP (TEE) treatments; B: The final PBS wash solution; C, E, G, I, K: 5 different batches of RP (TEE) solution diluted with PBS; D, F, H, J, L: PC-12 cell lysate solution collected after treatments of 5 different batches of RP (TEE). ( e ) The TIC of PC-12 cell lysate collected after 1 to 6 h of RP (TEE) treatments (500 μg/mL). ( f ) The TIC of PC-12 cell lysate collected after RP (TEE) treatments (125, 250, 500 or 750 μg/mL) for 4 h. All the samples were analyzed by UHPLC-TOF-MS on an <t>Agilent</t> Zorbax Eclipse Plus C-18 50 mm × 2.1 mm column (particle size: 1.8 μm) at a flow rate of 0.35 mL/min. The data was acquired in the scan mode from m/z 100 to 3200 Da with 2.0 spectra/s.
    Chromatographic Conditions High Performance Liquid Chromatography Grade Acetonitrile, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Waters Corporation chromatography conditions quantitative analysis
    The identification of the active binding fraction of RP by CMC. ( a ) The experimental flow of CMC. PC-12 cells were incubated with RP (TEE) for 1 to 6 h. After incubation, chemical components without binding affinity to the cell membrane were washed away by PBS, while those components that bind on cell membrane were retained for analysis. The cells were then disrupted by citric acid buffer with ultrasound sonication. The lysate solution was then centrifuged, dried and re-dissolved in methanol. Cell lysate without RP incubation was collected as control. Finally, all the collected samples were analyzed using <t>UHPLC-TOF-MS.</t> ( b ) The Total Ion Chromatogram (TIC) of the 5 different batches of RP (TEE). ( c ) The TIC of the CMC samples. S1: The final PBS wash solution; S2: PC-12 lysate solution without RP (TEE) treatments; S3: RP (TEE) solution diluted with PBS; S4: PC-12 cell lysate solution collected after RP (TEE) treatments. The cluster of peaks (C5) indicated the chemical components that bind on the cell membrane of PC-12 cells. ( d ) The TIC of the CMC samples collected from 5 different batches of RP (TEE) treatments. A: PC-12 lysate solution without RP (TEE) treatments; B: The final PBS wash solution; C, E, G, I, K: 5 different batches of RP (TEE) solution diluted with PBS; D, F, H, J, L: PC-12 cell lysate solution collected after treatments of 5 different batches of RP (TEE). ( e ) The TIC of PC-12 cell lysate collected after 1 to 6 h of RP (TEE) treatments (500 μg/mL). ( f ) The TIC of PC-12 cell lysate collected after RP (TEE) treatments (125, 250, 500 or 750 μg/mL) for 4 h. All the samples were analyzed by UHPLC-TOF-MS on an <t>Agilent</t> Zorbax Eclipse Plus C-18 50 mm × 2.1 mm column (particle size: 1.8 μm) at a flow rate of 0.35 mL/min. The data was acquired in the scan mode from m/z 100 to 3200 Da with 2.0 spectra/s.
    Chromatography Conditions Quantitative Analysis, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies chromatography conditions hplc
    The identification of the active binding fraction of RP by CMC. ( a ) The experimental flow of CMC. PC-12 cells were incubated with RP (TEE) for 1 to 6 h. After incubation, chemical components without binding affinity to the cell membrane were washed away by PBS, while those components that bind on cell membrane were retained for analysis. The cells were then disrupted by citric acid buffer with ultrasound sonication. The lysate solution was then centrifuged, dried and re-dissolved in methanol. Cell lysate without RP incubation was collected as control. Finally, all the collected samples were analyzed using <t>UHPLC-TOF-MS.</t> ( b ) The Total Ion Chromatogram (TIC) of the 5 different batches of RP (TEE). ( c ) The TIC of the CMC samples. S1: The final PBS wash solution; S2: PC-12 lysate solution without RP (TEE) treatments; S3: RP (TEE) solution diluted with PBS; S4: PC-12 cell lysate solution collected after RP (TEE) treatments. The cluster of peaks (C5) indicated the chemical components that bind on the cell membrane of PC-12 cells. ( d ) The TIC of the CMC samples collected from 5 different batches of RP (TEE) treatments. A: PC-12 lysate solution without RP (TEE) treatments; B: The final PBS wash solution; C, E, G, I, K: 5 different batches of RP (TEE) solution diluted with PBS; D, F, H, J, L: PC-12 cell lysate solution collected after treatments of 5 different batches of RP (TEE). ( e ) The TIC of PC-12 cell lysate collected after 1 to 6 h of RP (TEE) treatments (500 μg/mL). ( f ) The TIC of PC-12 cell lysate collected after RP (TEE) treatments (125, 250, 500 or 750 μg/mL) for 4 h. All the samples were analyzed by UHPLC-TOF-MS on an <t>Agilent</t> Zorbax Eclipse Plus C-18 50 mm × 2.1 mm column (particle size: 1.8 μm) at a flow rate of 0.35 mL/min. The data was acquired in the scan mode from m/z 100 to 3200 Da with 2.0 spectra/s.
    Chromatography Conditions Hplc, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Waters Corporation high performance liquid chromatography hplc conditions chromatographic analysis
    The identification of the active binding fraction of RP by CMC. ( a ) The experimental flow of CMC. PC-12 cells were incubated with RP (TEE) for 1 to 6 h. After incubation, chemical components without binding affinity to the cell membrane were washed away by PBS, while those components that bind on cell membrane were retained for analysis. The cells were then disrupted by citric acid buffer with ultrasound sonication. The lysate solution was then centrifuged, dried and re-dissolved in methanol. Cell lysate without RP incubation was collected as control. Finally, all the collected samples were analyzed using <t>UHPLC-TOF-MS.</t> ( b ) The Total Ion Chromatogram (TIC) of the 5 different batches of RP (TEE). ( c ) The TIC of the CMC samples. S1: The final PBS wash solution; S2: PC-12 lysate solution without RP (TEE) treatments; S3: RP (TEE) solution diluted with PBS; S4: PC-12 cell lysate solution collected after RP (TEE) treatments. The cluster of peaks (C5) indicated the chemical components that bind on the cell membrane of PC-12 cells. ( d ) The TIC of the CMC samples collected from 5 different batches of RP (TEE) treatments. A: PC-12 lysate solution without RP (TEE) treatments; B: The final PBS wash solution; C, E, G, I, K: 5 different batches of RP (TEE) solution diluted with PBS; D, F, H, J, L: PC-12 cell lysate solution collected after treatments of 5 different batches of RP (TEE). ( e ) The TIC of PC-12 cell lysate collected after 1 to 6 h of RP (TEE) treatments (500 μg/mL). ( f ) The TIC of PC-12 cell lysate collected after RP (TEE) treatments (125, 250, 500 or 750 μg/mL) for 4 h. All the samples were analyzed by UHPLC-TOF-MS on an <t>Agilent</t> Zorbax Eclipse Plus C-18 50 mm × 2.1 mm column (particle size: 1.8 μm) at a flow rate of 0.35 mL/min. The data was acquired in the scan mode from m/z 100 to 3200 Da with 2.0 spectra/s.
    High Performance Liquid Chromatography Hplc Conditions Chromatographic Analysis, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore conditioned sep pak c18 reverse chromatography cartridge
    The identification of the active binding fraction of RP by CMC. ( a ) The experimental flow of CMC. PC-12 cells were incubated with RP (TEE) for 1 to 6 h. After incubation, chemical components without binding affinity to the cell membrane were washed away by PBS, while those components that bind on cell membrane were retained for analysis. The cells were then disrupted by citric acid buffer with ultrasound sonication. The lysate solution was then centrifuged, dried and re-dissolved in methanol. Cell lysate without RP incubation was collected as control. Finally, all the collected samples were analyzed using <t>UHPLC-TOF-MS.</t> ( b ) The Total Ion Chromatogram (TIC) of the 5 different batches of RP (TEE). ( c ) The TIC of the CMC samples. S1: The final PBS wash solution; S2: PC-12 lysate solution without RP (TEE) treatments; S3: RP (TEE) solution diluted with PBS; S4: PC-12 cell lysate solution collected after RP (TEE) treatments. The cluster of peaks (C5) indicated the chemical components that bind on the cell membrane of PC-12 cells. ( d ) The TIC of the CMC samples collected from 5 different batches of RP (TEE) treatments. A: PC-12 lysate solution without RP (TEE) treatments; B: The final PBS wash solution; C, E, G, I, K: 5 different batches of RP (TEE) solution diluted with PBS; D, F, H, J, L: PC-12 cell lysate solution collected after treatments of 5 different batches of RP (TEE). ( e ) The TIC of PC-12 cell lysate collected after 1 to 6 h of RP (TEE) treatments (500 μg/mL). ( f ) The TIC of PC-12 cell lysate collected after RP (TEE) treatments (125, 250, 500 or 750 μg/mL) for 4 h. All the samples were analyzed by UHPLC-TOF-MS on an <t>Agilent</t> Zorbax Eclipse Plus C-18 50 mm × 2.1 mm column (particle size: 1.8 μm) at a flow rate of 0.35 mL/min. The data was acquired in the scan mode from m/z 100 to 3200 Da with 2.0 spectra/s.
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    Waters Corporation uplc qtof ms conditions chromatography
    The identification of the active binding fraction of RP by CMC. ( a ) The experimental flow of CMC. PC-12 cells were incubated with RP (TEE) for 1 to 6 h. After incubation, chemical components without binding affinity to the cell membrane were washed away by PBS, while those components that bind on cell membrane were retained for analysis. The cells were then disrupted by citric acid buffer with ultrasound sonication. The lysate solution was then centrifuged, dried and re-dissolved in methanol. Cell lysate without RP incubation was collected as control. Finally, all the collected samples were analyzed using <t>UHPLC-TOF-MS.</t> ( b ) The Total Ion Chromatogram (TIC) of the 5 different batches of RP (TEE). ( c ) The TIC of the CMC samples. S1: The final PBS wash solution; S2: PC-12 lysate solution without RP (TEE) treatments; S3: RP (TEE) solution diluted with PBS; S4: PC-12 cell lysate solution collected after RP (TEE) treatments. The cluster of peaks (C5) indicated the chemical components that bind on the cell membrane of PC-12 cells. ( d ) The TIC of the CMC samples collected from 5 different batches of RP (TEE) treatments. A: PC-12 lysate solution without RP (TEE) treatments; B: The final PBS wash solution; C, E, G, I, K: 5 different batches of RP (TEE) solution diluted with PBS; D, F, H, J, L: PC-12 cell lysate solution collected after treatments of 5 different batches of RP (TEE). ( e ) The TIC of PC-12 cell lysate collected after 1 to 6 h of RP (TEE) treatments (500 μg/mL). ( f ) The TIC of PC-12 cell lysate collected after RP (TEE) treatments (125, 250, 500 or 750 μg/mL) for 4 h. All the samples were analyzed by UHPLC-TOF-MS on an <t>Agilent</t> Zorbax Eclipse Plus C-18 50 mm × 2.1 mm column (particle size: 1.8 μm) at a flow rate of 0.35 mL/min. The data was acquired in the scan mode from m/z 100 to 3200 Da with 2.0 spectra/s.
    Uplc Qtof Ms Conditions Chromatography, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Shimadzu Corporation chromatography conditions hplc analysis
    The identification of the active binding fraction of RP by CMC. ( a ) The experimental flow of CMC. PC-12 cells were incubated with RP (TEE) for 1 to 6 h. After incubation, chemical components without binding affinity to the cell membrane were washed away by PBS, while those components that bind on cell membrane were retained for analysis. The cells were then disrupted by citric acid buffer with ultrasound sonication. The lysate solution was then centrifuged, dried and re-dissolved in methanol. Cell lysate without RP incubation was collected as control. Finally, all the collected samples were analyzed using <t>UHPLC-TOF-MS.</t> ( b ) The Total Ion Chromatogram (TIC) of the 5 different batches of RP (TEE). ( c ) The TIC of the CMC samples. S1: The final PBS wash solution; S2: PC-12 lysate solution without RP (TEE) treatments; S3: RP (TEE) solution diluted with PBS; S4: PC-12 cell lysate solution collected after RP (TEE) treatments. The cluster of peaks (C5) indicated the chemical components that bind on the cell membrane of PC-12 cells. ( d ) The TIC of the CMC samples collected from 5 different batches of RP (TEE) treatments. A: PC-12 lysate solution without RP (TEE) treatments; B: The final PBS wash solution; C, E, G, I, K: 5 different batches of RP (TEE) solution diluted with PBS; D, F, H, J, L: PC-12 cell lysate solution collected after treatments of 5 different batches of RP (TEE). ( e ) The TIC of PC-12 cell lysate collected after 1 to 6 h of RP (TEE) treatments (500 μg/mL). ( f ) The TIC of PC-12 cell lysate collected after RP (TEE) treatments (125, 250, 500 or 750 μg/mL) for 4 h. All the samples were analyzed by UHPLC-TOF-MS on an <t>Agilent</t> Zorbax Eclipse Plus C-18 50 mm × 2.1 mm column (particle size: 1.8 μm) at a flow rate of 0.35 mL/min. The data was acquired in the scan mode from m/z 100 to 3200 Da with 2.0 spectra/s.
    Chromatography Conditions Hplc Analysis, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 85/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies chromatographic conditions hplc system
    The identification of the active binding fraction of RP by CMC. ( a ) The experimental flow of CMC. PC-12 cells were incubated with RP (TEE) for 1 to 6 h. After incubation, chemical components without binding affinity to the cell membrane were washed away by PBS, while those components that bind on cell membrane were retained for analysis. The cells were then disrupted by citric acid buffer with ultrasound sonication. The lysate solution was then centrifuged, dried and re-dissolved in methanol. Cell lysate without RP incubation was collected as control. Finally, all the collected samples were analyzed using <t>UHPLC-TOF-MS.</t> ( b ) The Total Ion Chromatogram (TIC) of the 5 different batches of RP (TEE). ( c ) The TIC of the CMC samples. S1: The final PBS wash solution; S2: PC-12 lysate solution without RP (TEE) treatments; S3: RP (TEE) solution diluted with PBS; S4: PC-12 cell lysate solution collected after RP (TEE) treatments. The cluster of peaks (C5) indicated the chemical components that bind on the cell membrane of PC-12 cells. ( d ) The TIC of the CMC samples collected from 5 different batches of RP (TEE) treatments. A: PC-12 lysate solution without RP (TEE) treatments; B: The final PBS wash solution; C, E, G, I, K: 5 different batches of RP (TEE) solution diluted with PBS; D, F, H, J, L: PC-12 cell lysate solution collected after treatments of 5 different batches of RP (TEE). ( e ) The TIC of PC-12 cell lysate collected after 1 to 6 h of RP (TEE) treatments (500 μg/mL). ( f ) The TIC of PC-12 cell lysate collected after RP (TEE) treatments (125, 250, 500 or 750 μg/mL) for 4 h. All the samples were analyzed by UHPLC-TOF-MS on an <t>Agilent</t> Zorbax Eclipse Plus C-18 50 mm × 2.1 mm column (particle size: 1.8 μm) at a flow rate of 0.35 mL/min. The data was acquired in the scan mode from m/z 100 to 3200 Da with 2.0 spectra/s.
    Chromatographic Conditions Hplc System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 88/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies mass spectrometry conditions chromatography
    The identification of the active binding fraction of RP by CMC. ( a ) The experimental flow of CMC. PC-12 cells were incubated with RP (TEE) for 1 to 6 h. After incubation, chemical components without binding affinity to the cell membrane were washed away by PBS, while those components that bind on cell membrane were retained for analysis. The cells were then disrupted by citric acid buffer with ultrasound sonication. The lysate solution was then centrifuged, dried and re-dissolved in methanol. Cell lysate without RP incubation was collected as control. Finally, all the collected samples were analyzed using <t>UHPLC-TOF-MS.</t> ( b ) The Total Ion Chromatogram (TIC) of the 5 different batches of RP (TEE). ( c ) The TIC of the CMC samples. S1: The final PBS wash solution; S2: PC-12 lysate solution without RP (TEE) treatments; S3: RP (TEE) solution diluted with PBS; S4: PC-12 cell lysate solution collected after RP (TEE) treatments. The cluster of peaks (C5) indicated the chemical components that bind on the cell membrane of PC-12 cells. ( d ) The TIC of the CMC samples collected from 5 different batches of RP (TEE) treatments. A: PC-12 lysate solution without RP (TEE) treatments; B: The final PBS wash solution; C, E, G, I, K: 5 different batches of RP (TEE) solution diluted with PBS; D, F, H, J, L: PC-12 cell lysate solution collected after treatments of 5 different batches of RP (TEE). ( e ) The TIC of PC-12 cell lysate collected after 1 to 6 h of RP (TEE) treatments (500 μg/mL). ( f ) The TIC of PC-12 cell lysate collected after RP (TEE) treatments (125, 250, 500 or 750 μg/mL) for 4 h. All the samples were analyzed by UHPLC-TOF-MS on an <t>Agilent</t> Zorbax Eclipse Plus C-18 50 mm × 2.1 mm column (particle size: 1.8 μm) at a flow rate of 0.35 mL/min. The data was acquired in the scan mode from m/z 100 to 3200 Da with 2.0 spectra/s.
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    The identification of the active binding fraction of RP by CMC. ( a ) The experimental flow of CMC. PC-12 cells were incubated with RP (TEE) for 1 to 6 h. After incubation, chemical components without binding affinity to the cell membrane were washed away by PBS, while those components that bind on cell membrane were retained for analysis. The cells were then disrupted by citric acid buffer with ultrasound sonication. The lysate solution was then centrifuged, dried and re-dissolved in methanol. Cell lysate without RP incubation was collected as control. Finally, all the collected samples were analyzed using UHPLC-TOF-MS. ( b ) The Total Ion Chromatogram (TIC) of the 5 different batches of RP (TEE). ( c ) The TIC of the CMC samples. S1: The final PBS wash solution; S2: PC-12 lysate solution without RP (TEE) treatments; S3: RP (TEE) solution diluted with PBS; S4: PC-12 cell lysate solution collected after RP (TEE) treatments. The cluster of peaks (C5) indicated the chemical components that bind on the cell membrane of PC-12 cells. ( d ) The TIC of the CMC samples collected from 5 different batches of RP (TEE) treatments. A: PC-12 lysate solution without RP (TEE) treatments; B: The final PBS wash solution; C, E, G, I, K: 5 different batches of RP (TEE) solution diluted with PBS; D, F, H, J, L: PC-12 cell lysate solution collected after treatments of 5 different batches of RP (TEE). ( e ) The TIC of PC-12 cell lysate collected after 1 to 6 h of RP (TEE) treatments (500 μg/mL). ( f ) The TIC of PC-12 cell lysate collected after RP (TEE) treatments (125, 250, 500 or 750 μg/mL) for 4 h. All the samples were analyzed by UHPLC-TOF-MS on an Agilent Zorbax Eclipse Plus C-18 50 mm × 2.1 mm column (particle size: 1.8 μm) at a flow rate of 0.35 mL/min. The data was acquired in the scan mode from m/z 100 to 3200 Da with 2.0 spectra/s.

    Journal: Scientific Reports

    Article Title: Identification of novel autophagic Radix Polygalae fraction by cell membrane chromatography and UHPLC-(Q)TOF-MS for degradation of neurodegenerative disease proteins

    doi: 10.1038/srep17199

    Figure Lengend Snippet: The identification of the active binding fraction of RP by CMC. ( a ) The experimental flow of CMC. PC-12 cells were incubated with RP (TEE) for 1 to 6 h. After incubation, chemical components without binding affinity to the cell membrane were washed away by PBS, while those components that bind on cell membrane were retained for analysis. The cells were then disrupted by citric acid buffer with ultrasound sonication. The lysate solution was then centrifuged, dried and re-dissolved in methanol. Cell lysate without RP incubation was collected as control. Finally, all the collected samples were analyzed using UHPLC-TOF-MS. ( b ) The Total Ion Chromatogram (TIC) of the 5 different batches of RP (TEE). ( c ) The TIC of the CMC samples. S1: The final PBS wash solution; S2: PC-12 lysate solution without RP (TEE) treatments; S3: RP (TEE) solution diluted with PBS; S4: PC-12 cell lysate solution collected after RP (TEE) treatments. The cluster of peaks (C5) indicated the chemical components that bind on the cell membrane of PC-12 cells. ( d ) The TIC of the CMC samples collected from 5 different batches of RP (TEE) treatments. A: PC-12 lysate solution without RP (TEE) treatments; B: The final PBS wash solution; C, E, G, I, K: 5 different batches of RP (TEE) solution diluted with PBS; D, F, H, J, L: PC-12 cell lysate solution collected after treatments of 5 different batches of RP (TEE). ( e ) The TIC of PC-12 cell lysate collected after 1 to 6 h of RP (TEE) treatments (500 μg/mL). ( f ) The TIC of PC-12 cell lysate collected after RP (TEE) treatments (125, 250, 500 or 750 μg/mL) for 4 h. All the samples were analyzed by UHPLC-TOF-MS on an Agilent Zorbax Eclipse Plus C-18 50 mm × 2.1 mm column (particle size: 1.8 μm) at a flow rate of 0.35 mL/min. The data was acquired in the scan mode from m/z 100 to 3200 Da with 2.0 spectra/s.

    Article Snippet: Instruments and chromatographic conditions UHPLC (Agilent Technologies 1290 Series) equipped with the time of flight MS (Agilent Technologies 6230) with a jet stream ion source was operated in negative ion mode during the UHPLC analysis.

    Techniques: Binding Assay, Flow Cytometry, Incubation, Sonication, Mass Spectrometry