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  • 95
    Millipore chondroitinase b
    Average and standard deviation of the response of the creep strain for the <t>chondroitinase</t> treated and control groups (n=6 per group).
    Chondroitinase B, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 106 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chondroitinase b/product/Millipore
    Average 95 stars, based on 106 article reviews
    Price from $9.99 to $1999.99
    chondroitinase b - by Bioz Stars, 2020-10
    95/100 stars
      Buy from Supplier

    89
    Seikagaku chondroitinase b
    DKO mice are DS-free. Two-day-old pups were metabolically labeled with 35 S sulfate. Skin decorin CS/DS was extracted and purified (A-C). A) CS/DS chains were analyzed on a size-permeation Superose 6 column. The HS degraded products, which were proportionally increased in the DKO mice, derive from the HS-PGs which co-eluted with decorin in the size permeation column used for PGs separation (see “ Material and methods ”). B) Split products obtained after <t>chondroitinase</t> B treatment were size separated on a Superdex Peptide column and C) the iduronic acid content was calculated. The iduronic acid analysis was conducted in duplicate samples. D) Skin PGs were extracted from E19.5 embryos of different genotypes. Decorin was stained before and after chondroitinase ABC and B treatment.
    Chondroitinase B, supplied by Seikagaku, used in various techniques. Bioz Stars score: 89/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chondroitinase b/product/Seikagaku
    Average 89 stars, based on 35 article reviews
    Price from $9.99 to $1999.99
    chondroitinase b - by Bioz Stars, 2020-10
    89/100 stars
      Buy from Supplier

    89
    IBEX Technologies chondroitinase b
    Pharmacological knockdown of other glycosaminoglycan (GAG) family members does not affect Aβ 1-40 -mediated ROS production in VSMC. Primary human cerebral VSMC were pre-treated with <t>chondroitinase</t> B (10 -1 IU/mL; selectively degrades dermatin sulfate; panel a ), chondroitinase AC (10 -1 IU/mL; selectively degrades chondroitin sulfate; panel a ), or varying concentrations of the sulfation inhibitor sodium chlorate (5-50 mM; panel b ) for 2 h, washed, loaded with Mitotracker Red CM-H 2 XRos (MTR; 5 μM), and treated with Aβ 1-40 . In some cases, cells were pre-treated with heat-inactivated (HI) enzyme (at the same concentration of active enzyme) and washed prior to MTR loading and Aβ treatment. Fluorescence was measured after 30 minutes. Results are representative of 3 independent experiments performed in triplicate. *p
    Chondroitinase B, supplied by IBEX Technologies, used in various techniques. Bioz Stars score: 89/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chondroitinase b/product/IBEX Technologies
    Average 89 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
    chondroitinase b - by Bioz Stars, 2020-10
    89/100 stars
      Buy from Supplier

    93
    R&D Systems chondroitinase b
    Pharmacological knockdown of other glycosaminoglycan (GAG) family members does not affect Aβ 1-40 -mediated ROS production in VSMC. Primary human cerebral VSMC were pre-treated with <t>chondroitinase</t> B (10 -1 IU/mL; selectively degrades dermatin sulfate; panel a ), chondroitinase AC (10 -1 IU/mL; selectively degrades chondroitin sulfate; panel a ), or varying concentrations of the sulfation inhibitor sodium chlorate (5-50 mM; panel b ) for 2 h, washed, loaded with Mitotracker Red CM-H 2 XRos (MTR; 5 μM), and treated with Aβ 1-40 . In some cases, cells were pre-treated with heat-inactivated (HI) enzyme (at the same concentration of active enzyme) and washed prior to MTR loading and Aβ treatment. Fluorescence was measured after 30 minutes. Results are representative of 3 independent experiments performed in triplicate. *p
    Chondroitinase B, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chondroitinase b/product/R&D Systems
    Average 93 stars, based on 35 article reviews
    Price from $9.99 to $1999.99
    chondroitinase b - by Bioz Stars, 2020-10
    93/100 stars
      Buy from Supplier

    Image Search Results


    Average and standard deviation of the response of the creep strain for the chondroitinase treated and control groups (n=6 per group).

    Journal:

    Article Title: FRICTIONAL RESPONSE OF BOVINE ARTICULAR CARTILAGE UNDER CREEP LOADING FOLLOWING PROTEOGLYCAN DIGESTION WITH CHONDROITINASE ABC

    doi:

    Figure Lengend Snippet: Average and standard deviation of the response of the creep strain for the chondroitinase treated and control groups (n=6 per group).

    Article Snippet: This protocol for chondroitinase digestion was motivated by that of Schmidt et al. [ ].

    Techniques: Standard Deviation

    Average and standard deviation of the time-dependent friction coefficient μ eff for the chondroitinase treated (solid line) and control (dotted line) groups (n=6 per group).

    Journal:

    Article Title: FRICTIONAL RESPONSE OF BOVINE ARTICULAR CARTILAGE UNDER CREEP LOADING FOLLOWING PROTEOGLYCAN DIGESTION WITH CHONDROITINASE ABC

    doi:

    Figure Lengend Snippet: Average and standard deviation of the time-dependent friction coefficient μ eff for the chondroitinase treated (solid line) and control (dotted line) groups (n=6 per group).

    Article Snippet: This protocol for chondroitinase digestion was motivated by that of Schmidt et al. [ ].

    Techniques: Standard Deviation

    DKO mice are DS-free. Two-day-old pups were metabolically labeled with 35 S sulfate. Skin decorin CS/DS was extracted and purified (A-C). A) CS/DS chains were analyzed on a size-permeation Superose 6 column. The HS degraded products, which were proportionally increased in the DKO mice, derive from the HS-PGs which co-eluted with decorin in the size permeation column used for PGs separation (see “ Material and methods ”). B) Split products obtained after chondroitinase B treatment were size separated on a Superdex Peptide column and C) the iduronic acid content was calculated. The iduronic acid analysis was conducted in duplicate samples. D) Skin PGs were extracted from E19.5 embryos of different genotypes. Decorin was stained before and after chondroitinase ABC and B treatment.

    Journal: PLoS ONE

    Article Title: Dermatan Sulfate-Free Mice Display Embryological Defects and Are Neonatal Lethal Despite Normal Lymphoid and Non-Lymphoid Organogenesis

    doi: 10.1371/journal.pone.0140279

    Figure Lengend Snippet: DKO mice are DS-free. Two-day-old pups were metabolically labeled with 35 S sulfate. Skin decorin CS/DS was extracted and purified (A-C). A) CS/DS chains were analyzed on a size-permeation Superose 6 column. The HS degraded products, which were proportionally increased in the DKO mice, derive from the HS-PGs which co-eluted with decorin in the size permeation column used for PGs separation (see “ Material and methods ”). B) Split products obtained after chondroitinase B treatment were size separated on a Superdex Peptide column and C) the iduronic acid content was calculated. The iduronic acid analysis was conducted in duplicate samples. D) Skin PGs were extracted from E19.5 embryos of different genotypes. Decorin was stained before and after chondroitinase ABC and B treatment.

    Article Snippet: Quantification and spatial arrangement of IdoA along the chain was analyzed by cleaving 40,000 dpm of CS/DS with 2 mIU of chondroitinase B (Seikagaku) in 20 mM Hepes, pH 7.2, 50 mM NaCl, 4 mM CaCl2 , and 0.1 mg/ml BSA for 90 min.

    Techniques: Mouse Assay, Metabolic Labelling, Labeling, Purification, Staining

    Pharmacological knockdown of other glycosaminoglycan (GAG) family members does not affect Aβ 1-40 -mediated ROS production in VSMC. Primary human cerebral VSMC were pre-treated with chondroitinase B (10 -1 IU/mL; selectively degrades dermatin sulfate; panel a ), chondroitinase AC (10 -1 IU/mL; selectively degrades chondroitin sulfate; panel a ), or varying concentrations of the sulfation inhibitor sodium chlorate (5-50 mM; panel b ) for 2 h, washed, loaded with Mitotracker Red CM-H 2 XRos (MTR; 5 μM), and treated with Aβ 1-40 . In some cases, cells were pre-treated with heat-inactivated (HI) enzyme (at the same concentration of active enzyme) and washed prior to MTR loading and Aβ treatment. Fluorescence was measured after 30 minutes. Results are representative of 3 independent experiments performed in triplicate. *p

    Journal: Molecular Neurodegeneration

    Article Title: Heparan sulfate proteoglycans mediate Aβ-induced oxidative stress and hypercontractility in cultured vascular smooth muscle cells

    doi: 10.1186/s13024-016-0073-8

    Figure Lengend Snippet: Pharmacological knockdown of other glycosaminoglycan (GAG) family members does not affect Aβ 1-40 -mediated ROS production in VSMC. Primary human cerebral VSMC were pre-treated with chondroitinase B (10 -1 IU/mL; selectively degrades dermatin sulfate; panel a ), chondroitinase AC (10 -1 IU/mL; selectively degrades chondroitin sulfate; panel a ), or varying concentrations of the sulfation inhibitor sodium chlorate (5-50 mM; panel b ) for 2 h, washed, loaded with Mitotracker Red CM-H 2 XRos (MTR; 5 μM), and treated with Aβ 1-40 . In some cases, cells were pre-treated with heat-inactivated (HI) enzyme (at the same concentration of active enzyme) and washed prior to MTR loading and Aβ treatment. Fluorescence was measured after 30 minutes. Results are representative of 3 independent experiments performed in triplicate. *p

    Article Snippet: Chondroitinase B and AC were purchased from IBEX Technologies Inc. (Montreal, Quebec, Canada).

    Techniques: Concentration Assay, Fluorescence