chicken protein tyrosine kinase 2 Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92
    Millipore anti vegfr 2
    VEGF-A was required for CREB phosphorylation and protection induced by 24 h carotid-artery ligation preconditioning. A , Compared with vehicle or scrambled ODN, intracerebroventricular infusions of VEGF-A antisense ODN (VEGF-A AS) markedly reduced VEGF-A, <t>VEGFR-2,</t> and pCREB expression, but it did not affect VEGFR-1 levels of the preconditioned rat pups. # p
    Anti Vegfr 2, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti vegfr 2/product/Millipore
    Average 92 stars, based on 46 article reviews
    Price from $9.99 to $1999.99
    anti vegfr 2 - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    92
    Cell Signaling Technology Inc rabbit antibody against chicken p 44 42 mapk erk1 2 thr202 tyr204
    VEGF-A was required for CREB phosphorylation and protection induced by 24 h carotid-artery ligation preconditioning. A , Compared with vehicle or scrambled ODN, intracerebroventricular infusions of VEGF-A antisense ODN (VEGF-A AS) markedly reduced VEGF-A, <t>VEGFR-2,</t> and pCREB expression, but it did not affect VEGFR-1 levels of the preconditioned rat pups. # p
    Rabbit Antibody Against Chicken P 44 42 Mapk Erk1 2 Thr202 Tyr204, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit antibody against chicken p 44 42 mapk erk1 2 thr202 tyr204/product/Cell Signaling Technology Inc
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    rabbit antibody against chicken p 44 42 mapk erk1 2 thr202 tyr204 - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    94
    Cell Signaling Technology Inc rabbit anti chicken phosphor p44 42 mapk
    VEGF-A was required for CREB phosphorylation and protection induced by 24 h carotid-artery ligation preconditioning. A , Compared with vehicle or scrambled ODN, intracerebroventricular infusions of VEGF-A antisense ODN (VEGF-A AS) markedly reduced VEGF-A, <t>VEGFR-2,</t> and pCREB expression, but it did not affect VEGFR-1 levels of the preconditioned rat pups. # p
    Rabbit Anti Chicken Phosphor P44 42 Mapk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti chicken phosphor p44 42 mapk/product/Cell Signaling Technology Inc
    Average 94 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    rabbit anti chicken phosphor p44 42 mapk - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    Image Search Results


    VEGF-A was required for CREB phosphorylation and protection induced by 24 h carotid-artery ligation preconditioning. A , Compared with vehicle or scrambled ODN, intracerebroventricular infusions of VEGF-A antisense ODN (VEGF-A AS) markedly reduced VEGF-A, VEGFR-2, and pCREB expression, but it did not affect VEGFR-1 levels of the preconditioned rat pups. # p

    Journal: The Journal of Neuroscience

    Article Title: VEGF-A/VEGFR-2 Signaling Leading to cAMP Response Element-Binding Protein Phosphorylation Is a Shared Pathway Underlying the Protective Effect of Preconditioning on Neurons and Endothelial Cells

    doi: 10.1523/JNEUROSCI.5497-08.2009

    Figure Lengend Snippet: VEGF-A was required for CREB phosphorylation and protection induced by 24 h carotid-artery ligation preconditioning. A , Compared with vehicle or scrambled ODN, intracerebroventricular infusions of VEGF-A antisense ODN (VEGF-A AS) markedly reduced VEGF-A, VEGFR-2, and pCREB expression, but it did not affect VEGFR-1 levels of the preconditioned rat pups. # p

    Article Snippet: The following primary antibodies were used: anti-VEGF-A (1:2000; Oncogene), anti-VEGFR-1 (1:400; Santa Cruz Biotechnology), anti-VEGFR-2 (1:1000; Millipore Bioscience Research Reagents), anti-pCREB (1: 1000; Upstate), anti-CREB (1:2000; Upstate), and anti-actin (1:5000; Millipore Bioscience Research Reagents).

    Techniques: Ligation, Expressing

    VEGFR-2, but not VEGFR-1, was required for CREB phosphorylation and the protective effects of 24 h carotid-artery ligation preconditioning. A , Compared with vehicle or scrambled ODN, VEGFR-1 AS significantly reduced VEGFR-1 expression, but it did not alter VEGFR-2 or pCREB expression in the 24 h preconditioned group. B , In contrast, VEGFR-2 AS markedly reduced VEGFR-2 and pCREB, but not VEGFR-1, expression in the preconditioned group. * p

    Journal: The Journal of Neuroscience

    Article Title: VEGF-A/VEGFR-2 Signaling Leading to cAMP Response Element-Binding Protein Phosphorylation Is a Shared Pathway Underlying the Protective Effect of Preconditioning on Neurons and Endothelial Cells

    doi: 10.1523/JNEUROSCI.5497-08.2009

    Figure Lengend Snippet: VEGFR-2, but not VEGFR-1, was required for CREB phosphorylation and the protective effects of 24 h carotid-artery ligation preconditioning. A , Compared with vehicle or scrambled ODN, VEGFR-1 AS significantly reduced VEGFR-1 expression, but it did not alter VEGFR-2 or pCREB expression in the 24 h preconditioned group. B , In contrast, VEGFR-2 AS markedly reduced VEGFR-2 and pCREB, but not VEGFR-1, expression in the preconditioned group. * p

    Article Snippet: The following primary antibodies were used: anti-VEGF-A (1:2000; Oncogene), anti-VEGFR-1 (1:400; Santa Cruz Biotechnology), anti-VEGFR-2 (1:1000; Millipore Bioscience Research Reagents), anti-pCREB (1: 1000; Upstate), anti-CREB (1:2000; Upstate), and anti-actin (1:5000; Millipore Bioscience Research Reagents).

    Techniques: Ligation, Expressing

    Preconditioning induced by 24 h carotid-artery ligation increased VEGFR-2 and pCREB expression in neurons and vessels of the ipsilateral cerebral cortex. A , Immunohistochemistry showed that the 24 h ligation (preconditioned) group had higher VEGFR-2, but not VEGFR-1, expression in neurons (arrowheads, insets) and vessels (arrows) compared with the 1 h ligation (non-preconditioned) group and the nonligation control group (counterstained with hematoxylin). B , Immunofluorescence analysis showed most of the VEGFR-2 + nonvascular cells (arrowheads) of the preconditioned group coexpressed NeuN but not GFAP. These VEGFR-2 + cells also coexpressed VEGF-A. C , The 24 h preconditioned group had higher expression of VEGFR-2 and pCREB in vessels and neurons compared with the control or 1 h non-preconditioned group. The neurons (arrowheads) and vessels (arrows) with higher VEGFR-2 expression in the preconditioned group also coexpressed pCREB.

    Journal: The Journal of Neuroscience

    Article Title: VEGF-A/VEGFR-2 Signaling Leading to cAMP Response Element-Binding Protein Phosphorylation Is a Shared Pathway Underlying the Protective Effect of Preconditioning on Neurons and Endothelial Cells

    doi: 10.1523/JNEUROSCI.5497-08.2009

    Figure Lengend Snippet: Preconditioning induced by 24 h carotid-artery ligation increased VEGFR-2 and pCREB expression in neurons and vessels of the ipsilateral cerebral cortex. A , Immunohistochemistry showed that the 24 h ligation (preconditioned) group had higher VEGFR-2, but not VEGFR-1, expression in neurons (arrowheads, insets) and vessels (arrows) compared with the 1 h ligation (non-preconditioned) group and the nonligation control group (counterstained with hematoxylin). B , Immunofluorescence analysis showed most of the VEGFR-2 + nonvascular cells (arrowheads) of the preconditioned group coexpressed NeuN but not GFAP. These VEGFR-2 + cells also coexpressed VEGF-A. C , The 24 h preconditioned group had higher expression of VEGFR-2 and pCREB in vessels and neurons compared with the control or 1 h non-preconditioned group. The neurons (arrowheads) and vessels (arrows) with higher VEGFR-2 expression in the preconditioned group also coexpressed pCREB.

    Article Snippet: The following primary antibodies were used: anti-VEGF-A (1:2000; Oncogene), anti-VEGFR-1 (1:400; Santa Cruz Biotechnology), anti-VEGFR-2 (1:1000; Millipore Bioscience Research Reagents), anti-pCREB (1: 1000; Upstate), anti-CREB (1:2000; Upstate), and anti-actin (1:5000; Millipore Bioscience Research Reagents).

    Techniques: Ligation, Expressing, Immunohistochemistry, Immunofluorescence

    OGD preconditioning upregulated VEGF-A, VEGFR-2, and pCREB expression in H19-7 cells and b.End3 cells. Western blots and densitometric analysis showed that VEGF-A levels were significantly higher at 1 h and continued to rise for 24 h after OGD preconditioning in the H19-7 cells and b.End3 cells. The VEGFR-2 levels were higher 1 h after OGD preconditioning in the H19-7 cells and b.End3 cells, and the higher levels were sustained for 24 h in H19-7 cells and for 12 h in b.End3 cells after preconditioning. pCREB levels were higher 1 h after preconditioning in H19-7 cells and 3 h after preconditioning in b.End3 cells, and the increased pCREB levels persisted for at least 24 h after preconditioning in both types of cells. * p

    Journal: The Journal of Neuroscience

    Article Title: VEGF-A/VEGFR-2 Signaling Leading to cAMP Response Element-Binding Protein Phosphorylation Is a Shared Pathway Underlying the Protective Effect of Preconditioning on Neurons and Endothelial Cells

    doi: 10.1523/JNEUROSCI.5497-08.2009

    Figure Lengend Snippet: OGD preconditioning upregulated VEGF-A, VEGFR-2, and pCREB expression in H19-7 cells and b.End3 cells. Western blots and densitometric analysis showed that VEGF-A levels were significantly higher at 1 h and continued to rise for 24 h after OGD preconditioning in the H19-7 cells and b.End3 cells. The VEGFR-2 levels were higher 1 h after OGD preconditioning in the H19-7 cells and b.End3 cells, and the higher levels were sustained for 24 h in H19-7 cells and for 12 h in b.End3 cells after preconditioning. pCREB levels were higher 1 h after preconditioning in H19-7 cells and 3 h after preconditioning in b.End3 cells, and the increased pCREB levels persisted for at least 24 h after preconditioning in both types of cells. * p

    Article Snippet: The following primary antibodies were used: anti-VEGF-A (1:2000; Oncogene), anti-VEGFR-1 (1:400; Santa Cruz Biotechnology), anti-VEGFR-2 (1:1000; Millipore Bioscience Research Reagents), anti-pCREB (1: 1000; Upstate), anti-CREB (1:2000; Upstate), and anti-actin (1:5000; Millipore Bioscience Research Reagents).

    Techniques: Expressing, Western Blot

    Immunohistochemical distribution of VEGFR‐2 and B 1 receptors (B1R). In control retina (A), B 1 receptor immunostaining was very weak and the overall B 1 receptor immunofluorescence intensity was higher in STZ‐retina (D, D′), particularly in the GCL of diabetic retina. Compared to control retina (B), VEGFR‐2 immunofluorescence intensity increased in diabetic retina (E) notably in Müller cells process and in blood vessels (E′ arrow). There is no co‐localization between VEGFR‐2 and B 1 receptors in control and STZ‐retina on Müller cells and in GCL layer (C, F), but both co‐localized on blood vessels in STZ‐retina (F′). Bar scale: 75 μm.

    Journal: British Journal of Pharmacology

    Article Title: Expression, distribution and function of kinin B1 receptor in the rat diabetic retina) Expression, distribution and function of kinin B1 receptor in the rat diabetic retina

    doi: 10.1111/bph.14138

    Figure Lengend Snippet: Immunohistochemical distribution of VEGFR‐2 and B 1 receptors (B1R). In control retina (A), B 1 receptor immunostaining was very weak and the overall B 1 receptor immunofluorescence intensity was higher in STZ‐retina (D, D′), particularly in the GCL of diabetic retina. Compared to control retina (B), VEGFR‐2 immunofluorescence intensity increased in diabetic retina (E) notably in Müller cells process and in blood vessels (E′ arrow). There is no co‐localization between VEGFR‐2 and B 1 receptors in control and STZ‐retina on Müller cells and in GCL layer (C, F), but both co‐localized on blood vessels in STZ‐retina (F′). Bar scale: 75 μm.

    Article Snippet: Sections were incubated overnight at RT with the blocking buffer containing one of the following primary antibodies: polyclonal rabbit antiserum to rat B1 receptor 1:150, mouse monoclonal anti‐endothelial cells [rat endothelial cell antigen‐1 (RECA‐1), ab 9774; ABCAM] 1:500, mouse monoclonal anti‐glial fibrillary acid protein (GFAP) (IF03L, Millipore Sigma) 1:500 to label astrocytes, mouse polyclonal anti‐ionized calcium binding adapter molecule 1 (Iba‐1; Wako, Richmond, VA, USA) 1:500 (2 μg·mL−1 ) to label microglia and chicken monoclonal anti‐VEGFR‐2 (GW21181, Sigma‐Aldrich) 1:250.

    Techniques: Immunohistochemistry, Immunostaining, Immunofluorescence

    Relative mRNA expression of kinin receptors and the VEGF system in the retina at different time points of diabetes. mRNA levels of B 1 receptors (B1R; A, A'), B 2 receptors (B2R; B), VEGF‐A (C) and VEGFR‐2 (D) in control (Ctl; fold = 1) and STZ‐retina at 2 weeks (open graphs), 6 weeks (black graphs) and 6 months (A', grey graphs, B1R only) with or without intravitreal administration of the B 1 receptor agonist R‐838. (E) The impact of one intravitreal administration of B 1 receptor siRNA (0.5, 1.0 or 10 nmol) on mRNA levels of B 1 receptors, B 2 receptors, VEGF‐A and VEGFR‐2 in STZ‐retina at 2 weeks in comparison to control (Ctl, fold = 1.0). Data are mean ± SEM of values from five to six rats in each group. Statistical comparison to Ctl‐vehicle (*) and between 2 and 6 weeks (+) or between STZ‐vehicle and STZ‐R‐838 ( ) is indicated by * ,+, P

    Journal: British Journal of Pharmacology

    Article Title: Expression, distribution and function of kinin B1 receptor in the rat diabetic retina) Expression, distribution and function of kinin B1 receptor in the rat diabetic retina

    doi: 10.1111/bph.14138

    Figure Lengend Snippet: Relative mRNA expression of kinin receptors and the VEGF system in the retina at different time points of diabetes. mRNA levels of B 1 receptors (B1R; A, A'), B 2 receptors (B2R; B), VEGF‐A (C) and VEGFR‐2 (D) in control (Ctl; fold = 1) and STZ‐retina at 2 weeks (open graphs), 6 weeks (black graphs) and 6 months (A', grey graphs, B1R only) with or without intravitreal administration of the B 1 receptor agonist R‐838. (E) The impact of one intravitreal administration of B 1 receptor siRNA (0.5, 1.0 or 10 nmol) on mRNA levels of B 1 receptors, B 2 receptors, VEGF‐A and VEGFR‐2 in STZ‐retina at 2 weeks in comparison to control (Ctl, fold = 1.0). Data are mean ± SEM of values from five to six rats in each group. Statistical comparison to Ctl‐vehicle (*) and between 2 and 6 weeks (+) or between STZ‐vehicle and STZ‐R‐838 ( ) is indicated by * ,+, P

    Article Snippet: Sections were incubated overnight at RT with the blocking buffer containing one of the following primary antibodies: polyclonal rabbit antiserum to rat B1 receptor 1:150, mouse monoclonal anti‐endothelial cells [rat endothelial cell antigen‐1 (RECA‐1), ab 9774; ABCAM] 1:500, mouse monoclonal anti‐glial fibrillary acid protein (GFAP) (IF03L, Millipore Sigma) 1:500 to label astrocytes, mouse polyclonal anti‐ionized calcium binding adapter molecule 1 (Iba‐1; Wako, Richmond, VA, USA) 1:500 (2 μg·mL−1 ) to label microglia and chicken monoclonal anti‐VEGFR‐2 (GW21181, Sigma‐Aldrich) 1:250.

    Techniques: Expressing, CTL Assay

    Immunohistochemistry: Overexpressed VEGFR-2 cancer tissues were dyed brown in tumor cells. Notice that these nuclei did not react with A8H1. a human liver cancer tissue, b human prostate cancer tissue, c human colon cancer tissue, d human stomach cancer

    Journal: Cytotechnology

    Article Title: A high-affinity human/mouse cross-reactive monoclonal antibody, specific for VEGFR-2 linear and conformational epitopes

    doi: 10.1007/s10616-010-9262-4

    Figure Lengend Snippet: Immunohistochemistry: Overexpressed VEGFR-2 cancer tissues were dyed brown in tumor cells. Notice that these nuclei did not react with A8H1. a human liver cancer tissue, b human prostate cancer tissue, c human colon cancer tissue, d human stomach cancer

    Article Snippet: After being washed with PBS, the two kinds of cells were added with the purified ascites fluid of anti-VEGFR-2 mAb at 20 μg/ml at 4 °C overnight with SP2/0 blank antibody as the negative control, and incubated with 0.01% Rhodamine-conjugated antibodies against mouse IgG (Sigma–Aldrich) for 2 h at room temperature.

    Techniques: Immunohistochemistry

    SDS–PAGE: Purified recombinant VEGFR-2 proteins of 11 kDa and 37 kDa. M marker, 1 His-VEGFR-2 protein, 2 GST-VEGFR-2 protein

    Journal: Cytotechnology

    Article Title: A high-affinity human/mouse cross-reactive monoclonal antibody, specific for VEGFR-2 linear and conformational epitopes

    doi: 10.1007/s10616-010-9262-4

    Figure Lengend Snippet: SDS–PAGE: Purified recombinant VEGFR-2 proteins of 11 kDa and 37 kDa. M marker, 1 His-VEGFR-2 protein, 2 GST-VEGFR-2 protein

    Article Snippet: After being washed with PBS, the two kinds of cells were added with the purified ascites fluid of anti-VEGFR-2 mAb at 20 μg/ml at 4 °C overnight with SP2/0 blank antibody as the negative control, and incubated with 0.01% Rhodamine-conjugated antibodies against mouse IgG (Sigma–Aldrich) for 2 h at room temperature.

    Techniques: SDS Page, Purification, Recombinant, Marker

    ELISA: The reactivity of A8H1 with recombinant human VEGFR-2/Fc chimera at 2 μg/ml. The A8H1 could detect up to a level of 0.25 ng/ml

    Journal: Cytotechnology

    Article Title: A high-affinity human/mouse cross-reactive monoclonal antibody, specific for VEGFR-2 linear and conformational epitopes

    doi: 10.1007/s10616-010-9262-4

    Figure Lengend Snippet: ELISA: The reactivity of A8H1 with recombinant human VEGFR-2/Fc chimera at 2 μg/ml. The A8H1 could detect up to a level of 0.25 ng/ml

    Article Snippet: After being washed with PBS, the two kinds of cells were added with the purified ascites fluid of anti-VEGFR-2 mAb at 20 μg/ml at 4 °C overnight with SP2/0 blank antibody as the negative control, and incubated with 0.01% Rhodamine-conjugated antibodies against mouse IgG (Sigma–Aldrich) for 2 h at room temperature.

    Techniques: Enzyme-linked Immunosorbent Assay, Recombinant

    Western blot: a With 2 min exposure time, there were two bands of mature VEGFR-2 (230 kDa) and β-actin (43 kDa) visible in HUVEC, and only the β-actin was visible in NIH3T3 total protein samples. b With 10 min

    Journal: Cytotechnology

    Article Title: A high-affinity human/mouse cross-reactive monoclonal antibody, specific for VEGFR-2 linear and conformational epitopes

    doi: 10.1007/s10616-010-9262-4

    Figure Lengend Snippet: Western blot: a With 2 min exposure time, there were two bands of mature VEGFR-2 (230 kDa) and β-actin (43 kDa) visible in HUVEC, and only the β-actin was visible in NIH3T3 total protein samples. b With 10 min

    Article Snippet: After being washed with PBS, the two kinds of cells were added with the purified ascites fluid of anti-VEGFR-2 mAb at 20 μg/ml at 4 °C overnight with SP2/0 blank antibody as the negative control, and incubated with 0.01% Rhodamine-conjugated antibodies against mouse IgG (Sigma–Aldrich) for 2 h at room temperature.

    Techniques: Western Blot